LOCUS       NZ_JADBDR010000004     12542 bp    DNA     linear   CON 15-NOV-2023
DEFINITION  Paenibacillus sp. OAS669 Ga0395962_04, whole genome shotgun
            sequence.
ACCESSION   NZ_JADBDR010000004
VERSION     NZ_JADBDR010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus sp. OAS669
  ORGANISM  Paenibacillus sp. OAS669
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 451782)
  AUTHORS   Northen,T.
  TITLE     Enriched cells from switchgrass and soil in EcoFAB chamber, Walnut
            Creek, California, United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 451782)
  AUTHORS   Northen,T., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Pennacchio,C., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 196.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/15/2023 05:10:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,886
            CDSs (total)                      :: 6,766
            Genes (coding)                    :: 6,693
            CDSs (with protein)               :: 6,693
            Genes (RNA)                       :: 120
            rRNAs                             :: 11, 8, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 9, 8, 4 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 27 of 73
            Pseudo Genes (incomplete)         :: 56 of 73
            Pseudo Genes (internal stop)      :: 13 of 73
            Pseudo Genes (multiple problems)  :: 21 of 73
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:30:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 12542
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADBDR010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12542
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..12542
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12542
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1865:2542](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1866..2542
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            376..615
                     /locus_tag="GGC63_RS10495"
                     /old_locus_tag="GGC63_002174"
     CDS             376..615
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754944.1"
                     /locus_tag="GGC63_RS10495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002174"
                     /product="hypothetical protein"
                     /protein_id="WP_192702281.1"
                     /transl_table=11
                     /translation="MSQTGDDYGVLGSKIEADSDVNEDRHREAQLDDSFEAWYENIGDQ
                     VKQKLDLTNVPQDNVFESASDLDKDPLTDGNNLQ"
     gene            complement(665..829)
                     /locus_tag="GGC63_RS10500"
                     /old_locus_tag="GGC63_002175"
     CDS             complement(665..829)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013098.3"
                     /locus_tag="GGC63_RS10500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002175"
                     /product="VOC family protein"
                     /protein_id="WP_318781023.1"
                     /transl_table=11
                     /translation="MTMIELLTNDLLALHERIKQAGDPIDKEPVFTKDFGYFTFYDPDG
                     HYFRAVEEA"
     misc_feature    1067..1081
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 21.34"
                     /tool="antismash"
     gene            1075..1869
                     /locus_tag="GGC63_RS10505"
                     /old_locus_tag="GGC63_002176"
     CDS             1075..1869
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GGC63_RS10505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GGC63_002176"
                     /product="hypothetical protein"
                     /protein_id="WP_192702283.1"
                     /transl_table=11
                     /translation="MKKDYTNKQKYVNSYLITSNNNTTREIGAFVVAVSRILLYILEYG
                     ATGLFTFSIFRLLFGRNLYKILSITTIMSLLSYYLSEVAAMKGITIFFLPTFLIISCTV
                     LFNLPIAYSFLISIIYTIVGFLAEYLIASIAQLIGLTTYMGISNSLTQMSIIQISTSIL
                     LLLLTALFNYRKFGFMFLARQLYGRKALKPLNVIFGVLLILIVCAIQASRLSMYTATHS
                     MIHPIIFFLIALLLIIVLIIAWRRNKNAVDKKYLNTTGVGKK"
     gene            1866..2414
                     /locus_tag="GGC63_RS10510"
                     /old_locus_tag="GGC63_002177"
     CDS             1866..2414
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016526.3"
                     /locus_tag="GGC63_RS10510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002177"
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_192702284.1"
                     /sec_met_domain="AgrB (E-value: 1.8e-20, bitscore: 67.6,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPIDELASKIADFIRQNHPKASSKELLTYSLIITINTLTALLVS
                     FVICAVAGNLERWLVVFLILTVLRYFTGGAHLNSSLSCCIFTIIVFLLLVFVKYPYDYL
                     GLTFDIISLFVFAIYAPKGIDVIRKVSLRKKWIIKICSLLFIFSNFYLQNSLLSSIIIV
                     QAFSLTPWFEKIFKLERSY"
     gene            2417..2542
                     /locus_tag="GGC63_RS10515"
                     /old_locus_tag="GGC63_002178"
     CDS             2417..2542
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /locus_tag="GGC63_RS10515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002178"
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_192702285.1"
                     /sec_met_domain="TIGR04223 (E-value: 1.2e-08, bitscore:
                     29.5, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKKGIAKILNTMLVGVAFITVPTSFSFEWHRPEIPEELKK"
     gene            complement(2794..3573)
                     /locus_tag="GGC63_RS10520"
                     /old_locus_tag="GGC63_002180"
     CDS             complement(2794..3573)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594629.1"
                     /locus_tag="GGC63_RS10520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002180"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_192702286.1"
                     /sec_met_domain="PF07366 (E-value: 4.1e-06, bitscore: 21.2,
                     seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTFNHQHELVLKAIAVLESLESGNPEAITAYVHPEHYIQHNQALP
                     NGREAMLGALGHLKEIGTKVSIKRTLVDGDYVALHSLYDFHGPKAAFDIFRFENGLIVE
                     HWDNLQEMEGKTPSNHTMIDGPITIKDIDKTDANKLLVRSYVENILLGKNRDLLAAYFD
                     GDNYIQHSPHIADGLSGLHAALQALEEKHIEFQYTHVHQVIGQGDFVLTMSEGHFDRQP
                     TAFYDLFRVENGKIAEHWDVIEPILPAEKRKNSNPRF"
     gene            3766..4389
                     /locus_tag="GGC63_RS10525"
                     /old_locus_tag="GGC63_002181"
     CDS             3766..4389
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751913.1"
                     /locus_tag="GGC63_RS10525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002181"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_192702287.1"
                     /transl_table=11
                     /translation="MSKKTDLRILRTKQSIRKAFYELIQEKGYEAITVQDIADRAMINR
                     NTFYLHYQNKPDLLDTCMNDLLSELKDAIVLCPISTNPYSISVLELVMQTILEHISRNT
                     TFYYAMLVGENRIYPFRAKMENIIKDKLNEGWNPAKGNSPSSISKELLLEYLVSAFMGI
                     VIWWVKNDNALPADEVASQFSKIIAYGHLKAAGITVEETHPFPK"
     gene            complement(4453..5931)
                     /locus_tag="GGC63_RS10530"
                     /old_locus_tag="GGC63_002182"
     CDS             complement(4453..5931)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133547.1"
                     /locus_tag="GGC63_RS10530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002182"
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="WP_192702288.1"
                     /transl_table=11
                     /translation="MSMIQVQNLTFSYPSSFDNIFDDVSFQLDTDWKLGFIGRNGRGKT
                     TFFNLLLGKYEYSGKIISSVEFNYFPYPVSDKSRLTYEILEEVCPQAEDWEFLREISYL
                     DVDAEVMYRPFETLSNGEQTKVLLAALFLNEGQFLLIDEPTNHLDTDARKRVSNYLKRK
                     KGFILISHDRNFLDGCVDHILSINRANIEVQSGNYSSWRLNFDRQQEHEEAANERLQKD
                     IGRLQQSAKRSANWSDQVEASKNGTTNSGSKLDKGFVGHKAAKMMKRAKNIESRQQKAI
                     EEKSKLLKNVEKTESLKLEPLEYKSEELIRLTDVSVIYDGETVNKPISFHVKQGDRVVL
                     DGKNGSGKSSILKLILGEPLQHTGTLNVSSSLVISYVQQDTSHLKGMLSDFIEEHQIDE
                     PLFKSVLRKMDFERIQFEKDISHYSGGQKKKLLIAKSLCEKAHLYIWDEPLNFIDIYTR
                     MQIEELIQGFNPTMVFVEHDQAFQEQVATKTVAL"
     gene            complement(6103..7137)
                     /locus_tag="GGC63_RS10535"
                     /old_locus_tag="GGC63_002183"
     CDS             complement(6103..7137)
                     /EC_number="2.1.1.224"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735625.1"
                     /locus_tag="GGC63_RS10535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002183"
                     /product="Cfr family 23S rRNA
                     (adenine(2503)-C(8))-methyltransferase"
                     /protein_id="WP_079915025.1"
                     /sec_met_domain="PF04055 (E-value: 3.7e-16, bitscore: 54.6,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSTSKYEMIQHILTEWKQPAYRFQQITDAIFKQRIGEYDRMTVL
                     PKRIRNDFIMELGEHVLNLTPVMQKTSSQVNKVLFAIEGGERVEAVQLSYERGWKSYCI
                     SSQCGCGYGCRFCATGTIGLKRNLTADEITDQLLYFHLNGQALDSVSFMGMGEALANPN
                     IFDALKLLTDSRIFGLGHRRITVSTIGIMPGIDRLTQEFPQVNLTFSLHSPFDEQRSEL
                     MPINHRFPLHDVMNKLDQHIRTTGRKVYIAYILLRGENDSTEHAEAIAALLKNRGSWEH
                     LYHVNLIPYNATDVTPESFHPSDKDRVQRFVKILKSRGIHVTVRTQFGTDIQAACGQLY
                     GHNK"
     misc_feature    complement(7374..7383)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.04"
                     /tool="antismash"
     gene            7793..9199
                     /locus_tag="GGC63_RS10540"
                     /old_locus_tag="GGC63_002185"
     CDS             7793..9199
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673258.1"
                     /locus_tag="GGC63_RS10540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002185"
                     /product="hypothetical protein"
                     /protein_id="WP_192702289.1"
                     /transl_table=11
                     /translation="MFKTISVPNGLLTAALSASLLFTVPAHAAFGAEAPAEASAPIQTA
                     AVQTQQASSEYGVFLQEKFQVSLSETPTRGEFIAAVTRSLNFTPDSTTTVAFSDLSASS
                     PYYASAAALYQRGILTSTSVSADQPLSALQAAFIAVKAAGLTELAYSYPQSKVSQSLAP
                     LALNVDDFVSLSAAQEIAAAKDAGLVDPELYGDLKLGANASKRLTEQLLGHLLETQGQY
                     KHYIGQTSDPNIYAKLTDAYNTSDIIEAPELEKVVNTALEQGLVTGYNLKDSRFSSNFV
                     ESLSLTYGHSDLKHAIQLIGLLRSEGIEAKVQFEPKTSAFVHLIEWGQPTPSSTTRYQK
                     LANGNYIALAKEYDIAFEFTSTADKAKFQPIVLKYAKKNSEDTSGLIAGSWWQPLYYSL
                     TELNDYDVITNNKITTAAGPYYAQSFSLNDKSDGIVAGFKQIDPKVTVSTYKFWVDRPF
                     HNYLTGDDFK"
     gene            9391..9855
                     /locus_tag="GGC63_RS10545"
                     /old_locus_tag="GGC63_002186"
     CDS             9391..9855
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430571.1"
                     /locus_tag="GGC63_RS10545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GGC63_002186"
                     /product="phage tail protein"
                     /protein_id="WP_079915060.1"
                     /transl_table=11
                     /translation="MSYIVDFKNVSTVGLESSPVAEALAGLRANEARYFMNKYKHEFTV
                     VPAGESQEILDYVNRILKEERDIAFAAKPLETSRFQVENILFTYVFYEDGLGLNVMYTV
                     DDPKKRAVGFKLSEGMDVPKELEGKFKFARQKSKLAGTIRGSFFVIKGQY"
     misc_feature    complement(10823..10836)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     misc_feature    complement(11475..11489)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.07"
                     /tool="antismash"
     misc_feature    12434..12447
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.17"
                     /tool="antismash"
ORIGIN
        1 agccaccgaa aatcgtaata attcaggagg aaatacgcca aaagtaaagt acggctaggg
       61 aatttaatcc ctagccgtac tttgtcaaca gtcccgttcg gcggtttctt actcctttat
      121 ttcttcgtct tgccattgga tccggctacc tcagagtcct ggttatttcc aggctgagat
      181 tttttgccgt tattgttcgc attattgcga gacatccttc caacctcctt ttgctgagat
      241 ggcaacacga taaaagtatg ccactccttg gattccatat tcatctccga aatcttgcag
      301 aacaagtcca tgtataaaat ggattctttt gggagatttt aattagaccc aacaacgaag
      361 gagttgaagc actcaatgag ccaaactggg gatgattatg gggtacttgg cagtaaaatc
      421 gaagctgact cggatgtgaa tgaggatcgc catcgtgagg cgcaattaga tgactccttt
      481 gaagcctggt atgaaaacat cggcgatcag gtgaagcaaa agcttgatct tacgaatgtt
      541 ccgcaagaca atgtgtttga atctgcaagc gatttagaca aggacccttt aacggatggg
      601 aacaatttac aataaggaac aagccctcga gtctatcata agatactcaa gggctttact
      661 attgttatgc ttcttcgacc gctcgaaaat aatgcccgtc cggatcataa aaggtgaagt
      721 aaccgaaatc tttagtaaac acaggctctt tatcaatagg atcgccagct tgtttaatcc
      781 gttcatgcaa agcaagcaaa tcattcgtta atagctcaat catcgtcacg tgcccaaccg
      841 atcacatccg caccctttgt ttttttgttc aacataggcc acattctcct ttttatgttc
      901 tcagccagct ccacaataaa tccctcaggc ccacgaacgt aatcatccgc aagaacacat
      961 aatatgccgt tttaacgtca ttaagcccaa ctatccgatc aatcttataa ttgcaatttc
     1021 ttcctgtcaa taatttgttg ccaggcacaa agcgggcatt atttagttca cattgtgaaa
     1081 aaagactaca caaataaaca aaaatatgtt aattcttatt taataactag taacaataat
     1141 acaacaagag agattggtgc ctttgtggtt gctgtaagtc ggatattact atacatactg
     1201 gaatatggtg caacaggtct cttcaccttt tctatttttc gccttttgtt cggccgtaat
     1261 ctttataaaa tattatcaat tactactata atgagtctgc tctcctatta tttatcagaa
     1321 gtagcggcta tgaaagggat caccatcttc ttcctgccga catttttgat tatatcttgc
     1381 acagtgctgt ttaacctgcc gattgcatat tcattcctaa tttccatcat ttatacgatt
     1441 gtaggatttc tagcggaata cttaattgct tccattgcac agcttatagg tttaaccact
     1501 tatatgggta ttagtaattc attaactcaa atgtccatta ttcaaataag tactagtata
     1561 ttgcttctac tattaacagc tttgttcaat tacaggaagt ttggattcat gttcctcgca
     1621 agacaacttt acggtcgtaa agccttgaaa ccgctcaatg ttatttttgg cgttctactc
     1681 atattaattg tttgtgcaat tcaagcaagc aggctgtcta tgtacacggc tacacacagc
     1741 atgatccatc cgattatatt ttttttaatc gcattattac ttatcatcgt tttgataatt
     1801 gcctggagaa gaaacaaaaa cgcggtagat aaaaagtact taaacactac tggagtgggt
     1861 aaaaaatgaa tccaatcgat gagttagcct caaaaatagc tgactttatc aggcaaaatc
     1921 atcctaaagc ttcctctaag gagctgttaa cctattcgct aattattact attaataccc
     1981 ttaccgcact cttggtatca ttcgttattt gcgccgttgc cgggaacctg gaacgttggc
     2041 tcgtagtatt cttgatatta accgtattaa ggtactttac aggcggagcg catctaaatt
     2101 cttcgttatc ctgttgtatc tttaccatta tcgtattttt gcttcttgtt ttcgtaaagt
     2161 atccttatga ttacttagga ctcacttttg atattatttc tctatttgtt tttgcaatct
     2221 atgcaccaaa aggaattgat gtaataagga aggtctcttt acgtaaaaaa tggatcataa
     2281 aaatttgcag tttattattt attttttcca atttctactt acaaaattct ctattatcct
     2341 ctattataat tgtacaagca ttttcattaa caccttggtt tgaaaaaata tttaaacttg
     2401 agaggagtta ttgactatgg taaaaaaagg tatcgcgaaa attctgaata ccatgctagt
     2461 tggtgttgct tttattactg tacctacttc gtttagcttt gaatggcatc gtcctgagat
     2521 ccctgaagag cttaaaaagt aattcttcaa atgagtagaa ataggattgt aaaacaaggc
     2581 ctactgcagc aagcagtagg cctatgctta ttgtactcac gcattccatt caggaccaca
     2641 atcccttccg tacggcgcct cctgttcttt ttccgctcct tttttgcttg tggatcgcgg
     2701 acattttttt cgctttctcg accttcgtca tggtcgctct tgcgcggctc ccgtcgtcac
     2761 aaggaatcgt cgcgatgcag ttgcaaaatt tcctcaaaat ctagggttcg agttctttcg
     2821 tttttctgcc ggcagaatgg gctcgatgac atcccaatgc tcggcgatct ttccattctc
     2881 tacgcggaac aaatcgtaga aagcggtagg ctgacggtcg aagtgacctt cgctcattgt
     2941 aagcacaaaa tcgccttgtc ctatgacttg atggacatga gtatattgaa actcaatatg
     3001 cttctcctcc agcgcctgca atgcagcatg aagtccggaa aggccgtccg caatatgtgg
     3061 actatgctga atgtagttat ctccgtcaaa ataagcggca agcaggtcgc ggttcttccc
     3121 aagcaagatg ttctcgacgt agcttctgac caatagtttg ttggcatccg tcttgtcgat
     3181 atctttaatg gtgatcggtc catcaatcat cgtatgattg cttggtgtct tgccctccat
     3241 ctcttgtaaa ttatcccaat gctcgacgat gagtccattc tcgaaacgga aaatatcaaa
     3301 agcggcttta ggtccgtgaa aatcatataa agaatgaaga gcgacataat ctccgtctac
     3361 taatgtacgc ttaatgctta ccttcgtacc tatttccttt aaatgaccga gtgcgccaag
     3421 catggcttca cgaccattgg gtaaggcctg attgtgctga atataatgtt ccggatgaac
     3481 gtaagccgta atcgcttcgg ggttcccgct ttctaaactt tccagaacgg cgattgcttt
     3541 cagtacaagc tcatgttgat ggttgaatgt cattggaata tcctccttag agtaattggt
     3601 catttgataa ataatgaaca tcatgttgtt tatttatctg tcgctcatta taattgaggt
     3661 tattatcacg ttcaatattc aatatttttc gttttgaagg ataacagaca tcttataaaa
     3721 gtttgtcgct aaacggacta aatccatttt gggggaacga aaaccatgag caagaaaacg
     3781 gatctaagaa tactgcgcac gaaacaatcg attcgcaagg cgttttatga gcttattcag
     3841 gaaaaaggtt acgaagcgat tacggtacag gatattgccg atcgggcaat gattaatcgc
     3901 aacacctttt atctccatta ccaaaataag cccgatttat tagatacatg catgaatgat
     3961 ttgttgagcg aattaaagga tgccatcgtt ctttgtccca tcagtacgaa tccttacagt
     4021 atttctgtac tcgaactagt catgcaaact atactcgaac atatttcgcg taacactacc
     4081 ttttactatg ctatgttagt tggcgagaac agaatctatc cgtttcgagc gaaaatggag
     4141 aatatcatta aagataaact gaatgaagga tggaatcctg ctaagggaaa ttccccttcg
     4201 tcgatatcca aggaattgct gctcgaatac ctcgtatcag cttttatggg gattgtaatt
     4261 tggtgggtta aaaacgataa cgcccttcct gcagatgaag ttgcatccca gtttagcaaa
     4321 atcattgcct acgggcattt gaaagcggcc ggtatcacgg tcgaggaaac tcatccattt
     4381 cccaaataaa aaacaaaaat aaacccgctc cggaattatg gaacgggttt acttctcagt
     4441 actgaaggtt cattataggg ctaccgtttt cgtagccact tgttcttgga atgcctggtc
     4501 atgctctaca aacaccatgg ttggattgaa gccttgaata agctcttcaa tctgcatgcg
     4561 tgtataaata tcaatgaagt ttaacggctc atcccaaatg tataaatgag ctttttcaca
     4621 taagcttttg gctataagca gctttttctt ttgcccgccg gaataatggg atatatcctt
     4681 ttcaaactgg atacgttcaa agtccatttt tcgcagaacg gatttaaata acggttcatc
     4741 aatttgatgt tcttcaataa agtcggataa cattcccttt aaatgagaag tatcctgctg
     4801 tacataagaa atgacaagac ttgaactaac gttcaaagtg cctgtatgct gtaagggctc
     4861 ccctaggatg agctttaaaa tactgctttt tccactgcca ttctttccgt caagcacaac
     4921 ccggtctcct tgcttaacat gaaaacttat gggcttattc acggtttcac catcgtatat
     4981 aacggataca tccgttaaac gaatcagctc ttcggatttg tattccaaag gttcgagctt
     5041 taatgactct gttttttcca catttttgag cagctttgac ttttcttcaa tggctttctg
     5101 ctgtctggat tcaatattct ttgcacgttt catcattttg gcagccttgt gtcccacaaa
     5161 ccccttatcc aatttagaac cggaattcgt tgtgccattt ttggaagctt ctacttgatc
     5221 tgaccaattt gctgaacgtt tggcagattg ctgcagcctt ccaatgtctt tttgcaaacg
     5281 ctcgtttgct gcttcctcat gctcctgctg tctgtcgaaa ttgagcctcc aggaagaata
     5341 attaccgctt tgaacttcga tattcgcccg gttgatggac aaaatgtggt caacacagcc
     5401 atccaaaaag tttcgatcat gcgaaattaa aatgaaccct ttttttcttt ttaaataatt
     5461 ggaaaccctc tttcgtgcat cggtatccag gtgattggtg ggctcatcaa ttaataaaaa
     5521 ctgcccttca ttcaaaaaaa gcgcggcaag caaaaccttc gtttgttccc cattggataa
     5581 ggtttcaaag ggccggtaca tcacttcggc atcgacatcc aaataagaga tttcccgaag
     5641 aaactcccaa tcctccgcct gcggacatac ctcttccaat atttcatagg tgagtctgct
     5701 tttatccgag acagggtagg gaaaataatt aaactcgacc gaagaaataa ttttcccgct
     5761 atattcatat ttgcccagca acagattgaa aaacgttgtt ttaccccgtc cgttgcggcc
     5821 gataaatccg agcttccaat ccgtatcgag ttgaaagcta acatcgtcga aaatattatc
     5881 aaagctgctc ggataagaaa acgtcaggtt ttgtacttgt atcattgaca tgttgaaagt
     5941 tcctcctttt tataaccaca ctgcttcttt ataaaaagtc ggtacatatc catccagtct
     6001 cattttactc cgtataagtt taatggatat tatcgactta tacggagtaa atcatgcgta
     6061 accacggtgt ccgtactcat gttatccctc ctttagcttt ttttacttgt tatgtccata
     6121 taattggccg catgctgctt ggatatccgt tccgaattgg gttcgcacgg tgacatgaat
     6181 tcctctggac tttaaaatct taacaaaccg ttgaacccgg tctttgtccg atggatggaa
     6241 gctttcgggc gtcacatcgg tcgcattgta ggggatcaaa ttgacgtgat ataaatgttc
     6301 ccaagagcct ctattcttca acaaggcggc aatcgcctct gcgtgttccg ttgaatcgtt
     6361 ttctcctcga agcaagatat acgcaatata tacttttctg cctgtagtgc gaatatgctg
     6421 gtccagctta ttcattacat catgaagcgg gaagcgatgg ttaatgggca ttagctcgct
     6481 ccgctgttcg tcgaacggag aatgcaggga aaaggtcaga tttacctgcg gaaattcctg
     6541 cgtcagacga tcgatccccg gcataatacc gatagtggaa acggttattc ttcgatgacc
     6601 caagccgaag attcgggaat cggttagaag cttaagggcg tcgaagatat taggattggc
     6661 cagcgcctct cccatcccca taaacgaaac actatccaag gcttgaccgt ttaaatgaaa
     6721 atataacaat tgatcggtaa tttcatcggc agtcagattc cgtttcaggc cgattgtgcc
     6781 tgtagcgcaa aagcggcagc catatccgca tccgcactgg ctggaaatgc agtaggattt
     6841 ccacccgcgc tcatagctca gctgaacggc ttccacccgt tcgccgccct caatggcaaa
     6901 gagcacttta ttcacttgac tggatgtctt ttgcataaca ggggttaagt tcaaaacatg
     6961 ttcgccaagc tccatgatga aatcgtttct tatcctcttg ggcagtacgg tcattcggtc
     7021 atactccccg attctttgct taaagatcgc atccgtaatt tgctggaacc gataggcggg
     7081 ttgtttccat tctgtcaata tatgttgaat catttcgtat ttcgaagttg atttcatgtt
     7141 ttttctcctc ttgagccgga gaaaaagcct ttacaccctt ttgttggtgt ctaacaagca
     7201 cattaaccaa ccctatgaaa gcccacgaag cttgcagcta gaatcgctac gtgcatctat
     7261 cgatagatgg ataatgctcc ggcatccgtt ttatttgatc gtgaaccgta ccaacggaca
     7321 atgaacggct cacatgatca tttacaactt caaacatcga ttttcaacct ccttaaaaaa
     7381 ataaaaagag ctgcaagaaa gttccctttc ttgtagctca aataaatgaa tgctgaaatc
     7441 taacccacct cgtgggacag ataaatttat ttgagtgaag agaataagta actttcttgc
     7501 tcaacaaaaa ataaaacaga tattgtttta tcattcgtaa aatagcaaga aaacttacat
     7561 tattctcatc atctcccctc gaatactaaa agtgattgta gcataataac tttcacattt
     7621 caactttgcg tcatgcattc aattcgcgga tcggaacaga ttctctgact agatctcggc
     7681 tggttttcta ctattgacag gttttctgac gaatgataga atgcatagga aaatcctaca
     7741 taattactcg gatttatatt ttccatttta actatggttg gaggaatgca tcatgttcaa
     7801 gacgatttcc gtaccgaatg gactgctcac agcagcccta tccgcctccc tgctatttac
     7861 ggttccagcg cacgcggcat ttggcgctga agctccagcg gaagcttccg ctcccattca
     7921 gaccgccgct gtacaaactc agcaggcatc gagcgaatac ggcgtattcc tgcaggagaa
     7981 attccaagtg tcgctgtcgg aaacaccgac gcgcggggaa tttatcgctg ctgtcacccg
     8041 ctcgctgaac tttacgccgg atagtacaac gaccgtcgct ttcagcgatc tatcggcatc
     8101 cagtccgtac tacgcctcgg ccgcagcact gtatcagaga ggaattctga cctcgacgtc
     8161 cgtatcagcg gatcagccgc tctcagcgct gcaagccgcc ttcatcgcag taaaagcagc
     8221 gggcctcacc gagctggcct attcctatcc gcagtcgaaa gtgtcccaat cgctcgcccc
     8281 gctggcgctc aacgtcgatg atttcgtctc gctgtcggct gcccaggaga tcgctgcagc
     8341 taaggatgcc ggactcgtcg acccggaatt gtacggcgat ctgaagctgg gcgcgaacgc
     8401 ctccaaacgg ctaacggagc agcttctcgg acacttgctg gaaactcagg gccagtataa
     8461 acactacatc ggccaaacct ccgatcccaa tatctatgcg aagctgaccg atgcctacaa
     8521 tacatcggat atcatcgagg cgcctgaact ggagaaagtt gtcaacaccg ctttggagca
     8581 agggctggtc accggttata atttgaagga ctcgcgattc agctccaact tcgtcgaatc
     8641 gttatcccta acgtacggcc attccgatct aaagcacgcg attcagctga tcggcttgct
     8701 gcgcagcgaa ggtattgaag ccaaagtgca gtttgagccg aagacatccg ctttcgtaca
     8761 tctgatcgag tggggtcagc cgacaccgtc cagcacgacg cgctatcaaa agctggccaa
     8821 cggcaactat atcgccttgg cgaaggagta cgacatcgcc ttcgaattca cgagtacggc
     8881 ggacaaggcc aaattccagc cgatcgtact gaaatacgcc aagaaaaaca gcgaagacac
     8941 atccggcctg atcgctggtt cctggtggca gccgctctat tattccttga ccgaactgaa
     9001 cgattacgat gtgattacca ataataaaat caccactgca gctggtcctt attatgcgca
     9061 atcgttctcg ctgaacgaca agtcggacgg catcgtcgcc ggtttcaaac aaatcgaccc
     9121 gaaggtcacc gtcagcacgt ataagttctg ggtagaccgt ccgttccaca actatttgac
     9181 gggcgacgac tttaaatgag aacaatgctt atcgatatat tttcatagat gatgcagcga
     9241 aggtcacgat attgatttgg catggcttcc attcctcgaa ataaagggtt ttgacgtagc
     9301 aatgtagtat atttaaagtg tcgtttgtca ataccaatct atgtaaatat ctaagaggat
     9361 tcgggaataa agagaaaatg agggatggac atgtcgtata tcgttgattt taaaaatgtg
     9421 tctacggtag gtttggaatc ttcacctgta gcggaagcgc tcgctggttt acgtgctaac
     9481 gaagcccgtt actttatgaa caaatacaag catgaattta cggttgtgcc agctggcgaa
     9541 agtcaggaga tccttgatta tgtgaaccga atcttgaagg aggaacggga tattgcgttt
     9601 gcggccaagc ctttggaaac atcgcgcttt caagtcgaaa atatcctgtt cacctacgtt
     9661 ttttatgagg atggtcttgg gcttaatgtc atgtatacgg ttgacgatcc caagaagcgg
     9721 gctgttggct ttaagctttc tgagggaatg gacgttccca aggagttgga aggcaagttt
     9781 aagtttgcca ggcagaaatc caaattggcc ggaacgattc gaggctcgtt ttttgtaatt
     9841 aaaggacaat attaagcagg aaatagcgaa tcactaaaag agaagttcta tcaaaataag
     9901 ccactccatt gtgcggagtg gcttgttgtt tatgtgttga cttaattgga aggaaaacca
     9961 ttcgaactaa aggctacctc cttgcagata aatctactcc aagatcacct tattcgataa
    10021 cggattcggt aaggttccct ctatagaaga atcgagtttg tcgacgataa acacatgaac
    10081 ggtataggtg cccgcatccg catccgcatc cggcacatcg aaatgtcctg tcaggctgcc
    10141 accattcgtg agctcacttt ccagcgcaac atgggatacg ggagtgctgc ccttgagaag
    10201 ctgaaagtag acgacttctg tacccgcatg atctaacgac cctgctgtac gggctacgtc
    10261 aaccctagcg ctcaaaccgt tcgtccggtt cagcccgccg tttgcaatga cagtaaacgg
    10321 cttatccccc gcagcggtta cctttacagt gcgtactacc gggtccgccg cattttgtgc
    10381 gttgtccatt acggtataga ccagcgtgta cgttcctggc gtcgacgtgt ccacattgcc
    10441 gctcacttga atgcggcttg tcagatcgcc gtccacatca tccgccgccg tcgctcctgc
    10501 atccgtatac gtggtgccag ccaatacttc gatcactgcc ggcccgttca gggttatcac
    10561 cggcttcacc gaatccgcgg gtatggcggc aagatcggac acctgaaagt catcgatgcg
    10621 ggctgcctgg tttgccaccc ggatccccgc cttgccttgc atcagtgcgg gaggattcgt
    10681 cgggaataac gatccgttat ctacttcgtc atagatcagg ctatcgtcca catagccttt
    10741 gatccgattg ccgtccacga caagacgaag ctgataccag ttgccgaccg catacgggta
    10801 tgggattttt ttgctgtcca gctcacgcag cgtcccgttc acgacacgac caaggatgac
    10861 ataccccgtg cttccgccgg gcagtccatt cgtgccgtta tgaatgcgca gcatataaaa
    10921 gtttttggca tcgtcggatg cgcgcgcaat aatgcctggc agtgacgggc tggcctgcgc
    10981 tttaactctg gcccttacat cgtaatctgt ccaaccgctt tgaccgaccg taaaggccat
    11041 gctggtcgtc gtatacttct ccagcacctt gccctgatcc tcatccacaa tgcgtagatg
    11101 tgaagctgca ccattcggta cggcaccgtt attccaaccg gtcgctgcgt cctgattgta
    11161 agctccgctc tcaaaatttt cttcgtatag caccgtcccg gcagccgcct ccggattcgc
    11221 ccggatattc agtatatatt ccgccgtcgt tccggcttcg gaggttactt ttaccgttgc
    11281 ggtacccgag ccgttcacca aagctgcgtt agtgatttga actgaagcca aattgcttgc
    11341 cgtcgggatc gcctctacca ccttgacata agccgtacgg gaaggaacgt gaatcgtgta
    11401 tttcgtttga cccggaacca gagcaacact cgattcgggt aattgttggc ccggggctgt
    11461 cagagggtcc gtaatggccg acagcctcag gctgctaagc gatgtgtcgg cgcttgccgt
    11521 ccgcaagttt atcttgtaat aattcgcccc attgccttca gtcgacagcg cagctattgt
    11581 gtacgacctc tcttggccaa ccgcgtcgaa ggccacctgc ccggaggccg gggacgccat
    11641 ggtattggcc gtaccgtaga cggttgccgt cacgattacc gcaaagctcg tcgatgggac
    11701 aagcacagtg tagttgttca aatgcggatg gaagccctca atggaaacgc cgttcatctt
    11761 aatatcgcca agcagcggaa cgatcggata cactttgaca tcgtcgaagc ctacgatgga
    11821 ttcatggctt cgcagcccga atttaccgga agcccggctg gcatccgtaa gctcgataac
    11881 ctgaacgccg tcaatagaag ctcgcagcgt tgttccgaca gcttccaaat tcagcctata
    11941 ccagcggttc gactgaatcg gatcagccaa atccaccgac ttaagcacgg ttgtcgttcc
    12001 attgacgcgc ttctccagca ctgcctgatg cgcggagctg tttcggtaca gcttgaagta
    12061 atacaagtta ttgtaatcgg tcgctcgggc aataagcccc atttgcacgt ttccactgtc
    12121 cgtagttctg acccgcgctt ccactccata atccttaaga ctgtcaccaa aaatggcgaa
    12181 actaggtgtc gtgctgtata ggtccatcgg ttgcacgaat gtgttagggt aaacactgtc
    12241 cgtcacggtg gtccatgggg tggatttcga tccaaagcca accgtcgtcc atgccgccat
    12301 tgcattctta tcctcggtag tgtcgaaccc gtttttgtac agcgcttcgg caaaaacctt
    12361 tacatcgtta atcgatagcg ggccatcgaa ggtggagacc gtcaccgtca gcttcacata
    12421 ccgcgccttg gtttggaacc agtccaaact gttccctctt gggactgcgg aagccgggtc
    12481 ggtttgatcc gctaccgtat ggtacgacgt gccatccaag ctcgtcgcta ttgcgtagct
    12541 ga
//
