LOCUS       NZ_JACSPO010000008     10390 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Oceanitalea stevensii strain Sa1BUA1
            NODE_8_length_121011_cov_23.275489, whole genome shotgun sequence.
ACCESSION   NZ_JACSPO010000008
VERSION     NZ_JACSPO010000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Oceanitalea stevensii
  ORGANISM  Oceanitalea stevensii
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Bogoriellaceae; Georgenia.
REFERENCE   1  (bases 1 to 121011)
  AUTHORS   Gilroy,R., Ravi,A., Getino,M., Pursley,I., Horton,D.L.,
            Alikhan,N.-F., Baker,D., Gharbi,K., Hall,N., Watson,M.,
            Adriaenssens,E.M., Foster-Nyarko,E., Jarju,S., Secka,A., Antonio,M.,
            Oren,A., Chaudhuri,R., La Ragione,R.M., Hildebrand,F. and
            Pallen,M.J.
  TITLE     A Genomic Blueprint of the Chicken Gut Microbiome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 121011)
  AUTHORS   Gilroy,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) Microbes in the Food Chain, Quadram
            Institute BioScience, Norwich Research Park, Norwich NR4 7UQ, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 221x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014837105.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 01:02:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,251
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,182
            CDSs (with protein)               :: 3,182
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 0 of 16
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:07:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 38433
            Orig. end                         :: 48823
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACSPO010000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5390)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(985..1000)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.19"
                     /tool="antismash"
     gene            1081..2187
                     /gene="dapE"
                     /locus_tag="H9624_RS12695"
                     /old_locus_tag="H9624_12615"
     CDS             1081..2187
                     /EC_number="3.5.1.18"
                     /GO_function="GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA]"
                     /GO_process="GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /gene="dapE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1247: acetylornithine deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116238.1"
                     /locus_tag="H9624_RS12695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9624_12615"
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="WP_251840260.1"
                     /transl_table=11
                     /translation="MTAPALPDLRTADVVDLTRALCDIPSVSGEEGPLADAIEAALAGL
                     PHLEVLRDGDAVMARTRLGRERRAVVAGHIDTVPVAGNLPSELRTVDGEEVLWGRGSVD
                     MLGGVAVALHLAARLTAPAWDVTWVFYDNEEVEAERNGLGRLARHHPQWLAADFAVLGE
                     PSNGGIEGGCNGTLRVSVTTAGRAAHSARAWRGVNAIHLAAPILTRLAEYEPRSVEVDG
                     LVYREGLSAVGISGGIAGNVVPDSCEVLVNYRFAPSSSLAEAEAHVREVLAGYDVTVVD
                     GASGARPGLDAPGVGDFLAAVGRVTGGGPAPKYGWTDVARFAELGVPAVNFGPGDPSLA
                     HADDEHCPTAQIRDCATALEAWLTEGAR"
     gene            2184..3041
                     /locus_tag="H9624_RS12700"
                     /old_locus_tag="H9624_12620"
     CDS             2184..3041
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lysine_decarbox"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773817.1"
                     /locus_tag="H9624_RS12700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9624_12620"
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="WP_251840261.1"
                     /sec_met_domain="Lysine_decarbox (E-value: 7.7e-36,
                     bitscore: 116.0, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRSYNRNGSAGASREPERPSVRDSRDADVYRKGPVTLRGAQIPA
                     ETSDERLLAKDGDADWVHSDPWRVMRIQSEFVEGFGALAELGPAISVFGSARTGRDSAE
                     YRMGEEVGRLLVEAGYAVITGGGPGVMEAANKGACEAGGVSVGLGIELPFEQGMNEWVD
                     LGVNFRYFFARKTMFVKYALGFIVLPGGFGTMDELWEALTLVQTGKVNSFPIVLVGSEY
                     WGGLLAWARDTMVRAGTIAEADLDLIQVVDTAEEAVDRVRDRLALMRDEAESQARTAEE
                     ASTS"
     gene            3182..3352
                     /locus_tag="H9624_RS12705"
                     /old_locus_tag="H9624_12625"
     CDS             3182..3352
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883410.1"
                     /locus_tag="H9624_RS12705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9624_12625"
                     /product="DUF3117 domain-containing protein"
                     /protein_id="WP_110852098.1"
                     /transl_table=11
                     /translation="MAAMKPRTGDGPMEVTKEGRGIVMRVPLEGGGRLVVELTPAEAGE
                     LSEALSAVAPA"
     gene            3356..4912
                     /locus_tag="H9624_RS12710"
                     /old_locus_tag="H9624_12630"
     CDS             3356..4912
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883409.1"
                     /locus_tag="H9624_RS12710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9624_12630"
                     /product="M17 family metallopeptidase"
                     /protein_id="WP_251840262.1"
                     /transl_table=11
                     /translation="MTSLSELGLAELPEVVGEAGSLDGADLTQAGAVVVAVAPAGAGEE
                     GVEPRPGTADAAARYGIDLVDLAARHGLTGRAGESAVLDLPRALPGRDFGWNELPARLV
                     LLGVGDAGPDAMRKVGSALCRTTTGLGTVVTPIGALAGTTGLAALLEGFWLTAYRGPRM
                     AQSPRPGKPAASRLVLLGNDADTAVIAAARTAATATVRARFLAATPSNVKNPQWLAEQA
                     QALAAGVPGVSVEVHDEAWIRAQGMGALLAVGSGSATPPRLVTVTYSPRRRGGRTVALV
                     GKGITFDTGGISIKPRESMVGMKTDMAGAAAVLATVLAAAELQVPHRVVGVLALAENAF
                     GASSYRPGDVVRAYDGTSIEVSNTDAEGRMVLADAIAWVRDTARPDVLVDVATLTGAVT
                     LGLGRTHAGLYATDESLAAALQAAGEVSGERTWRLPLEDSYRHALDSAVADISHQARDP
                     HVSGGSITAALFLQHFAGDTPWAHLDIAGAGRATKARDEIPEGPTGYGARLLLRWLQDL
                     A"
     gene            complement(5001..5390)
                     /locus_tag="H9624_RS12715"
                     /old_locus_tag="H9624_12635"
     CDS             complement(5001..5390)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156041.1"
                     /locus_tag="H9624_RS12715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9624_12635"
                     /product="ectoine synthase"
                     /protein_id="WP_251840263.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.2e-56, bitscore:
                     182.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFHDLDDTEFDIQTPNWRSKRIILARDKVGFSVHETTLYAG
                     TENEFWYANHIEAVMIVEGEGTITDLGTGETHELRPGSMYLLNEHDKHVVRPRTEIRTI
                     CVFNPPVTGREVHDENGVYPLITEN"
     gene            complement(5393..6673)
                     /gene="ectB"
                     /locus_tag="H9624_RS12720"
                     /old_locus_tag="H9624_12640"
     CDS             complement(5393..6673)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 1.8e-69.
                     Score: 226.3. Matches aSDomain:
                     nrpspksdomains_H9624_RS12720_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512924.1"
                     /locus_tag="H9624_RS12720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9624_12640"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_251840264.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.1e-75, bitscore:
                     246.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITSPTTTTTELESEVRSYSRSWPVTFDRAVGATMYDTDGNPYI
                     DFFAGAGSLNYGHNNPALKKVLIDYLARDGVVHSLDMFTAARDEFLTTFADTILRPRGL
                     DHKVVFPGPGGANAVEAALKLARKVTGRESVISFTNAFHGMTLGALSVTGNAMKRGGAG
                     VPLVHATPMPYDDYFDGDVQDFLYLERLLTDSGGGLNQPAAVIVETIQGEGGINAARAE
                     WLRNLAGLCKRHGILLIVDDIQMGCGRTGGFFSFEEADIVPDIITLSKSISGYGLPMAL
                     TLVRPELDIWEPGEHNGTFRGFNPAFVTATEAIRRYWSDDALERSTISRGMAIESRFNA
                     LVARYESHGLVAKGRGLARGLQFPSGELAGRVTQEAFANGLLVETSGPGDEVVKLLPPL
                     TISPAEFEAGMRILEQAVVTVLENQEN"
     aSDomain        complement(5585..6589)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H9624_RS12720_Aminotran_3.1"
                     /evalue="1.80E-69"
                     /label="H9624_RS12720_Aminotran_3.1"
                     /locus_tag="H9624_RS12720"
                     /protein_end="363"
                     /protein_start="28"
                     /score="226.3"
                     /tool="antismash"
                     /translation="DRAVGATMYDTDGNPYIDFFAGAGSLNYGHNNPALKKVLIDYLAR
                     DGVVHSLDMFTAARDEFLTTFADTILRPRGLDHKVVFPGPGGANAVEAALKLARKVTGR
                     ESVISFTNAFHGMTLGALSVTGNAMKRGGAGVPLVHATPMPYDDYFDGDVQDFLYLERL
                     LTDSGGGLNQPAAVIVETIQGEGGINAARAEWLRNLAGLCKRHGILLIVDDIQMGCGRT
                     GGFFSFEEADIVPDIITLSKSISGYGLPMALTLVRPELDIWEPGEHNGTFRGFNPAFVT
                     ATEAIRRYWSDDALERSTISRGMAIESRFNALVARYESHGLVAKGRGLARGLQF"
     gene            complement(6721..7194)
                     /gene="ectA"
                     /locus_tag="H9624_RS12725"
                     /old_locus_tag="H9624_12645"
     CDS             complement(6721..7194)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779904.1"
                     /locus_tag="H9624_RS12725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9624_12645"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_251840265.1"
                     /transl_table=11
                     /translation="MTVRAPEPAEGADMWRLARDSGALDLNTSYAYLLLARDFARTCRV
                     AVVDGEVVGFLLGYRRPEEPSTLFVWQVAVDPAQRGRRLAARLLADAAAGAGFVEASIT
                     ADNTASQRLFESFAAERGAPLVRSELFAAADFPDAGHETEGLVRIGPLEPIES"
     gene            complement(7703..9004)
                     /locus_tag="H9624_RS12730"
                     /old_locus_tag="H9624_12650"
     CDS             complement(7703..9004)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779979.1"
                     /locus_tag="H9624_RS12730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9624_12650"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_251840266.1"
                     /transl_table=11
                     /translation="MASITLDNVVKTYPDGFTAVKGVSLDIADGEFVILVGPSGCGKST
                     LLRMIVGLEDISDGELRIADEVVNDKAPRDRDLAMVFQNYALYPHLTVYENIAFPLRLA
                     KGEYTDEQIDERVREASRLLELDEHLERKPANLSGGQRQRVAMGRAIVRRAKAFLFDEP
                     LSNLDAKLRGQMRTEIARLQRNLATTTVYVTHDQTEAMTLGDRVAVLRRGELQQVASPR
                     GLYDQPINLFVAGFIGSPPMNFLPGIVEGDVLRLPIVDVPMDERMRAAVGDREVVIVGI
                     RPDAFEDVDTMEPDRADAGVRLEVDVDVTEWLGEVLYAYIPFETHEAVQAQLEELDRDL
                     DGEGMRTQLIISLDAASTVRAGDPAELWFDPASLHLFDPESGDNLTRSEERAAELEDQA
                     RAMRERALERAKRRAEKDGQQDGERATQTARTRR"
     gene            complement(9030..9878)
                     /locus_tag="H9624_RS12735"
                     /old_locus_tag="H9624_12655"
     CDS             complement(9030..9878)
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1069: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881597.1"
                     /locus_tag="H9624_RS12735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H9624_12655"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_251840267.1"
                     /transl_table=11
                     /translation="MRGLSTKQKIGWVAITLVVLVWSLFPVVSIAMTSFKTQAGLFSGT
                     FLPEEWTLENYKTILAPGGSAQDLFLPSLRNSIGISLIATFIAVFLATLCAYAIARLDF
                     RGKRVILTTALAVSVFPVVSIVTPLFNLWRSIGLYDTWAGLVIPYLSLTLPISIWTLAA
                     FFRQIPWELEQAAQVDGATPFQAFRKAVVPLAAPGVFTTAIIAFFIAWNDFVYGISLTS
                     TSAARPVPAALSFFTGASQFEEPTGSISAAAIVVTVPVVIVVLLFQRRIVSGLTQGAVK
                     G"
ORIGIN
        1 tcatcgacgg caccggccgg ctggccggcc acctcggcag caccggcacc cggcccgtcc
       61 acgtgacgac cgccgacggc gaggagatcg agctcgtcgc cgagatcgtc ctcgtcgcca
      121 cgggcgccac cccccgcgtc ctgccggacg cgcagccgga cggcgagcgc atcctcacct
      181 gggcgcagat gtacaacctc accgagctgc ccgagcacct cgtcgtcgtc ggctccggcg
      241 tcaccggcgc cgagttcgcc ggcgcctaca acggcctcgg cgtgcccgtc accctcgtct
      301 ccagccgcga ccgggtgctg cccggggagg acgaggacgc cgccgagctc atcgagaacg
      361 tcttccgcag ccgcggcatg accgtcctgt cccgctcccg cgccgccgcc gtccgccgcg
      421 tcggggacgg cgtcgaggtc gagctcaccg acggccgcgt cgtcaccgcc tcccactgcc
      481 tcgtcgccgt cggcggcgtg ccgagcaccg cgggcctggg cctggaggag gccggtgtcg
      541 tgctccgcga cagcgggcac atccaggtcg accgcgtctc ccgcacgacg ggggtgcgcg
      601 tctacgccgc cggcgactgc accggcgtgc tgcccctcgc ctccgtggcg gcgatgcagg
      661 gccgcatcgc catgtggcac gcgctgggtg acgccgtctc cccgctcgac gtcgacgccg
      721 tcaccgccaa catcttcacc gcccccgaga tcgcgaccat cggcgtcggt gagcgggagg
      781 tgcacgacgg cgaggtcgac gccgtcatca cccagctgcc gctcgcccgc aacccgcggg
      841 cgaagatgct cggcatccgc gagggcttcg tcaaggtcat ggccgaccgg cactccgggg
      901 cggtgctcgg cgcggtcgtc gtcgcgccgc gcgccagcga gctcatcttc ccgctcgccg
      961 tcgccgtcac gcagcggatc acggtggacc agctcgccaa cgtctacacg atctacccct
     1021 cgctcagcgg gtcgatcgcg gaggccgccc gcacgctcca ccagacgggg gagtgagcgc
     1081 atgaccgccc ccgcgctccc ggacctgcgc acggccgacg tcgtcgacct cacccgcgcc
     1141 ctgtgcgaca tcccctccgt ctcgggcgag gaggggccgc tcgccgacgc gatcgaggcc
     1201 gctctcgccg gcctgccgca cctggaggtg ctgcgcgacg gtgacgccgt catggcgcgc
     1261 acccggctcg gccgcgagcg tcgggcggtc gtcgccggcc acatcgacac cgtgccggtc
     1321 gccggcaacc tgccgagcga gctgcgcacc gtcgacggcg aggaggtgct gtggggccgc
     1381 gggagcgtcg acatgctcgg cggcgtcgcc gtcgccctcc acctcgcggc gcggctcacc
     1441 gcacccgcgt gggacgtcac gtgggtgttc tacgacaacg aggaggtcga ggccgagcgc
     1501 aacgggctcg gccgcctcgc ccgccaccac ccgcagtggc tggccgccga cttcgccgtc
     1561 ctcggggagc cgagcaacgg cggcatcgag ggtggctgca acggcacgct gcgcgtgtcc
     1621 gtcacgacgg cgggacgcgc cgcccactcc gcccgcgcct ggcgcggggt caacgccatc
     1681 cacctcgccg cgcccatcct cacgcgcctg gccgagtacg agccgcgctc cgtcgaggtc
     1741 gacggcctcg tctaccgcga gggcctgtcc gcggtcggca tcagcggcgg catcgccggc
     1801 aacgtcgtgc ccgactcctg cgaggtgctc gtcaactacc gcttcgcgcc gtcgtcctcg
     1861 ctcgccgagg ccgaggcgca cgtgcgcgag gtgctcgccg gctacgacgt caccgtcgtc
     1921 gacggtgcct ccggggcccg ccccggcctc gacgcccccg gcgtgggcga cttcctcgcc
     1981 gccgtcgggc gcgtgacggg cggcggcccc gcccccaagt acgggtggac cgacgtcgcc
     2041 cgcttcgccg agctcggggt gccggccgtg aacttcgggc ccggtgaccc gtccctggcc
     2101 cacgccgacg acgagcactg ccccaccgcc cagatccgtg actgtgccac cgccctggag
     2161 gcgtggctga ccgaaggagc acgatgagcc ggtcctacaa ccgcaacggt tccgcaggcg
     2221 cgagccgcga acccgagcgc ccttccgtcc gcgacagccg tgacgccgac gtctaccgca
     2281 aggggcccgt gacgctgcgc ggcgcgcaga tccccgccga gacctccgac gagcgcctgc
     2341 tcgccaagga cggcgacgcc gactgggtcc actccgaccc gtggcgcgtc atgcggatcc
     2401 agagcgagtt cgtcgagggc ttcggcgcgc tcgccgagct cggccccgcc atcagcgtct
     2461 tcggctcggc gcggaccggt cgcgactccg cggagtaccg gatgggcgag gaggtcgggc
     2521 gcctgctcgt cgaggccggc tacgccgtca tcaccggagg tggccccggc gtcatggagg
     2581 ccgcgaacaa gggtgcctgc gaggccggcg gggtgtcggt gggcctgggc atcgagctgc
     2641 ccttcgagca gggcatgaac gagtgggtcg acctcggggt caacttccgc tacttcttcg
     2701 cccgcaagac gatgttcgtc aagtacgccc tcggcttcat cgtcctgccc ggcggcttcg
     2761 gcaccatgga cgagctgtgg gaggccctca ccctcgtcca gaccggcaag gtgaactcct
     2821 tcccgatcgt gctcgtcggc tctgagtact ggggcgggct gctggcctgg gcacgcgaca
     2881 ccatggtgcg ggcgggcacg atcgccgagg cggacctcga cctcatccag gtcgtcgaca
     2941 ccgccgagga ggccgtggac cgcgtccgtg accggctcgc gctcatgcgg gacgaggcgg
     3001 agtcgcaggc ccgcaccgcg gaggaggcct cgacctcctg agcgtcccgg gcgcccgtcg
     3061 cagcgccccg acctgcaccg ttgccccgag cgcacaccgc cgcgggggag gggtggacta
     3121 gactggtccc ggtgcgccgc agaccctgcg gcacgacacg tcgatcgaat aggggtgcca
     3181 gatggccgcg atgaagccga ggaccgggga cgggccgatg gaggtcacga aggagggacg
     3241 cggcatcgtc atgcgcgttc ccctcgaggg tggcggtcgc ctcgtcgtcg agctcacgcc
     3301 cgcggaggca ggcgagctca gcgaggccct cagcgccgtc gcccccgcct gaccgttgac
     3361 ctccctgagc gagctcggcc tcgccgagct tcccgaggtc gtcggtgagg ccggttcgct
     3421 cgacggcgcc gacctcaccc aggccggcgc cgtcgtcgtc gccgtcgcgc ccgccggcgc
     3481 cggcgaggag ggcgtcgagc cccgccccgg cacggcggac gccgccgcgc gctacggcat
     3541 cgacctcgtc gacctcgccg cgcgccacgg cctgaccgga cgggcggggg agtcggcggt
     3601 cctcgacctg ccgcgtgccc tgcccggccg cgacttcggc tggaacgagc tgccggcccg
     3661 cctggtgctc ctcggcgtcg gcgacgccgg cccggacgcc atgcgcaagg tcggctcggc
     3721 cctgtgccgc acgaccaccg ggctgggcac ggtcgtcacg cccatcggcg cgctcgccgg
     3781 caccaccggc ctggccgccc tgctcgaggg cttctggctc accgcctacc gcggcccgcg
     3841 catggcgcag tccccgcgac cgggcaagcc tgccgcctcc cggctggtcc tcctcggcaa
     3901 cgacgcggac accgccgtca tcgcggcggc gcgcacggcg gccaccgcca cggtgcgggc
     3961 ccgcttcctc gccgcgacgc cgtccaacgt caagaacccg cagtggctgg ccgagcaggc
     4021 ccaggcgctc gccgcgggtg tccccggcgt gagcgtcgag gtccacgacg aggcctggat
     4081 ccgggcgcag gggatgggcg cgctgctcgc cgtcggctcc ggctccgcga ccccgccgcg
     4141 gctcgtcacg gtcacctaca gcccgcgccg gcgcggcggg cggaccgtcg cgctcgtggg
     4201 caagggcatc accttcgaca ccggcggcat ctcgatcaag ccccgcgagt cgatggtcgg
     4261 catgaagacc gacatggccg gcgccgccgc ggtgctcgcg accgtcctcg ccgccgccga
     4321 gctgcaggtg ccccaccggg tggtgggcgt gctggccctg gcggagaacg ccttcggcgc
     4381 ctcctcctac cggcccggcg acgtcgtgcg cgcctacgac ggcacgtcga tcgaggtctc
     4441 caacaccgac gccgagggcc gcatggtcct ggccgacgcg atcgcctggg tgcgtgacac
     4501 cgcacggccc gacgtcctcg tcgacgtcgc cacgctcacc ggcgcggtga cgctgggcct
     4561 gggacggacc cacgccggcc tctacgcgac cgacgagtcc ctggccgccg ccctccaggc
     4621 ggcgggcgag gtgagcggcg agcggacgtg gcgcctgccc ctggaggact cctaccggca
     4681 cgccctggac tccgccgtcg ccgacatctc ccaccaggcg cgcgacccgc acgtctcggg
     4741 aggctcgatc accgcggccc tgttcctcca gcacttcgcg ggggacaccc cgtgggcgca
     4801 cctggacatc gccggcgccg gtcgcgcgac caaggcccgc gacgagatcc ccgagggccc
     4861 cacgggctac ggcgcccgcc tcctgctccg ctggctgcag gacctcgcct aacccccaac
     4921 ccagcgcata tgcgcgaaat ccgcggctcc ggagactccg gagccgcgga ttttccgcat
     4981 atgaggtgga gaggggggcg tcagttttcc gtgatcaagg ggtagacgcc gttctcgtcg
     5041 tggacctcgc ggccggtgac gggcgggttg aagacgcaga tcgtgcggat ctcggtacgc
     5101 ggccggacga cgtgcttgtc gtgctcgttg aggaggtaca tcgagcccgg acgcagctcg
     5161 tgcgtctcgc cggtgccgag gtcggtgatc gtgccctcgc cctcgacgat catcacggcc
     5221 tcgatgtggt tggcgtacca gaactcgttc tcggtgcccg cgtacagcgt ggtctcgtgc
     5281 accgagaagc ccaccttgtc ccgggcgagg atgatgcgct tcgagcgcca gttgggggtc
     5341 tggatgtcga actcggtgtc gtcgaggtcg tggaagctac gaacgatcat ggtcagttct
     5401 cctggttctc gaggacggtg acgacggcct gctcgaggat gcgcatgccg gcctcgaact
     5461 ccgccgggct gatggtgagc gggggcagca gcttgacgac ctcgtcgccc ggtccggacg
     5521 tctccacgag caggccgttc gcgaaggcct cctgggtgac ccggccggcg agctcgccgg
     5581 aggggaactg cagcccgcgg gccaggccgc ggcccttggc cacgaggccg tggctctcgt
     5641 agcgggcgac gagcgcgttg aagcgcgact cgatcgccat gccgcgggag atcgtgctgc
     5701 gctcgagcgc gtcgtcggac cagtagcgac ggatcgcctc ggtcgcggtg acgaaggcgg
     5761 ggttgaagcc tcggaaggtg ccgttgtgct cacccggctc ccagatgtcg agctcggggc
     5821 gcacgagggt cagcgccatc ggcaggccgt agccgctgat cgacttcgac agcgtgatga
     5881 tgtcggggac gatgtcggcc tcctcgaagc tgaagaagcc tccggtgcgg ccgcagccca
     5941 tctggatgtc gtcgacgatg agcaggatcc cgtgccgctt gcacaggccg gcgaggttgc
     6001 gcagccactc ggcgcgggcg gcgttgatcc cgccctcgcc ctggatggtc tcgacgatga
     6061 cggcggccgg ctggttgagg ccgccgcccg agtcggtgag cagccgctcg aggtagagga
     6121 agtcctggac gtcgccgtcg aagtagtcgt cgtagggcat cggtgtcgcg tggacgaggg
     6181 ggacgcccgc cccgccgcgc ttcatcgcgt tgccggtcac cgacagcgcg cccagggtca
     6241 tgccgtggaa ggcgttggtg aagctgatga ccgactcgcg gccggtgacc ttgcgggcga
     6301 gcttgagcgc cgcctcgacg gcgttggcgc cgcccggccc ggggaagacg accttgtggt
     6361 ccagtccgcg tgggcgcagg atcgtgtcgg cgaaggtcgt gaggaactcg tcgcgtgccg
     6421 cggtgaacat gtcgagcgag tgcacgacgc cgtcacgcgc gaggtagtcg atgagcacct
     6481 tcttcagggc cgggttgttg tgcccgtagt tgagcgagcc ggcgccggcg aagaagtcga
     6541 tgtaggggtt gccgtcggtg tcgtacatcg tcgcgccgac ggcgcggtcg aaggtgaccg
     6601 gccaggagcg ggagtagctg cggacctcgg actcgagctc ggtcgtggtc gtggtcgggc
     6661 tggtgatcgt catcggggag gctgcctttc gtggcggtac ggacgcgtgc gggtgtgggt
     6721 tcagctctcg atgggctcca gggggccgat cctcaccagc ccttcggtct cgtgtccggc
     6781 gtcggggaag tccgcggcgg cgaagagctc gctgcggacc agaggggcgc cgcgctcggc
     6841 ggcgaagctc tcgaagaggc gctgggaggc ggtgttgtcc gcggtgatcg aggcctccac
     6901 gaagccggcg ccggcggcgg cgtcggccag gagccgggcg gcgagccggc ggccgcgctg
     6961 ggccgggtcg acggcgacct gccagacgaa gagcgtgctc ggctcctcgg ggcggcggta
     7021 gccgaggagg aagcccacga cctcaccgtc gaccacggcc acccggcagg tgcgcgcgaa
     7081 gtcgcgcgcc agcaggaggt aggcgtagga ggtgttgagg tccagggcac cggagtcgcg
     7141 ggcgaggcgc cacatgtcgg cgccctcggc gggctccggg gcccggacgg tcacgggcgg
     7201 gtggccgctg cctgtgtcgg gtgcggcggc ggacgggaca cggggtgctg ctggtgtggc
     7261 ggggggcata cggccaacca cgttaacgag gtcccgcgcc gtccgacagc cgacgggagc
     7321 ggttcgtcgg cggccggggt cgtccgccgc gcgccgccgc accggccgta tcgcgtggca
     7381 ccgtgcggga aggtccgccc gtgctcgaga cgctttcggg aggggaaaat tgcgtccaca
     7441 tggtgagaaa tttcctcaag tgtgacctgg accatgcgca ccacccgccc gcggcgcccc
     7501 cgccggagtc agctcaccga gacgagacga gacgcggcga ggtgcggcgg agcgcggcga
     7561 gtggagcgag ccgcgccgat gactaccgcc cacccacccg ccccccacgt gccccacccc
     7621 acgtgcgccg acaactggat cccgcccggt gcgggatcca gctgtcggtg gcgagggggt
     7681 cggagcgggg cgggcgggtg ggtcagcggc gtgtgcgggc ggtctgcgtc gcgcgctcgc
     7741 cgtcctgctg gccgtccttc tccgcccgcc gcttggcccg ctcgagggcg cgctcgcgca
     7801 tcgcccgggc ctggtcctcg agctcggccg cgcgctcctc gctgcgggtg aggttgtcac
     7861 cggactccgg gtcgaagagg tggagggagg cggggtcgaa ccacagctcg gccgggtcgc
     7921 cggcgcggac ggtgctcgcc gcgtcgaggg agatgatgag ctgggtgcgc atgccctcgc
     7981 cgtcgaggtc ccggtccagc tcctcgagct gcgcctggac ggcctcgtgg gtctcgaagg
     8041 ggatgtaggc gtagagcacc tcaccgagcc actcggtgac gtcgacgtcg acctcgaggc
     8101 gcaccccggc gtcggcgcgg tcgggctcca tcgtgtcgac gtcctcgaag gcgtccgggc
     8161 ggataccgac gatgacgacc tcacggtcgc cgacggcggc gcgcatccgc tcgtccatgg
     8221 ggacgtcgac gatgggcagg cgcaggacgt cgccctcgac gatccccggc aggaagttca
     8281 tcggcgggga gccgatgaag ccggcgacga agaggttgat cggctggtcg tagagcccgc
     8341 gcgggctggc cacctgctgc agctcgccgc gccggagcac ggccacccgg tccccgaggg
     8401 tcatcgcctc ggtctggtcg tgggtgacgt agaccgtggt cgtggcgagg ttgcgctgca
     8461 ggcgggcgat ctcggtgcgc atctgcccgc ggagcttggc gtcgaggttg gacagcggct
     8521 cgtcgaagag gaaggccttg gcccgccgga cgatcgcgcg gcccatggcg acgcgctggc
     8581 gctgaccgcc ggagaggttg gcgggcttgc gctcgaggtg ctcgtcgagc tcgaggagcc
     8641 gggaggcctc ccggactcgc tcgtcgatct gctcgtcggt gtactcaccc ttggccaggc
     8701 gcagcgggaa ggcgatgttc tcgtagaccg tgaggtgggg gtacagcgcg tagttctgga
     8761 agaccatcgc gaggtcgcgg tcgcgcggcg ccttgtcgtt gacgacctcg tcggcgatgc
     8821 gcagctcgcc gtcgctgatg tcctccagcc cgacgatcat ccgcagcagg gtcgacttcc
     8881 cgcagccgga cgggcccacg aggatgacga actcgccgtc ggcgatgtcg aggctcacgc
     8941 ccttgaccgc ggtgaagccg tccgggtagg tcttgacgac gttgtcgagg gtgatcgatg
     9001 ccatggctgg actccttctc gttcgggcct cagcccttga ctgcgccttg ggtgaggccg
     9061 gacacgatgc gccgctggaa gagcaggacg acgatgacga ccggcacggt gacgacgatg
     9121 gccgccgccg agatcgagcc ggtgggctcc tcgaactgcg aggcgccggt gaagaaggac
     9181 agggccgccg gcacgggccg ggcggcgctc gtcgaggtga gcgagatgcc gtagacgaag
     9241 tcgttccacg cgatgaagaa ggcgatgatc gccgtcgtga acacgccggg ggcggcgagc
     9301 gggacgacgg ccttgcggaa ggcctggaac ggggtggcgc cgtcgacctg ggccgcctgc
     9361 tccagctccc aggggatctg ccggaagaag gccgccaggg tccagatgga gatcggcagg
     9421 gtgagggaca ggtaggggat gacgaggccg gcccaggtgt cgtacaggcc gatcgagcgc
     9481 cagaggttga acagcggcgt gacgatcgag acgacgggga agaccgacac ggccagggcg
     9541 gtggtgagga taacccgctt gccccggaag tccagccgcg cgatggcgta ggcgcacagc
     9601 gtcgcgagga agacggcgat gaaggtggcg atgagcgaga tgccgatgga gttgcgcagc
     9661 gagggcagga agaggtcctg ggcgctgccg ccgggcgcga ggatcgtctt gtagttctcc
     9721 agcgtccact cctcgggcag gaaggtcccg gagaagaggc cggcctgcgt cttgaacgag
     9781 gtcatggcga tggacacgac ggggaacagc gaccacacga ggacgacgag cgtgatcgcg
     9841 acccacccga tcttctgctt cgtggacagg cccctcatca cttgcctccc gtcgagccgg
     9901 cgagatcgac cttgaagccc ttgatcgcga tgcccgcgat gatgagcacg cagaggaaga
     9961 gcagcaccga gatcgccgag ccgaggccga tctcgagccg ccctatcgag gtgttgtagg
    10021 ccagcagcga gagcacctcg gtgtcgttcg cgccgttggt catgatgtag acgttgtcga
    10081 agatccggaa ggcctccagc gcccggaaga gcacggcgac catgatcgcg gccttcatgt
    10141 tcggcaggat gacccgcttg aggacctgcc agcccgtggc gccgtcgacc ttcgccgcct
    10201 cctcgaggtc cccgggcacc tgcgcgaggc cggacaggag gagcagggag atgaacgggg
    10261 tcgtcttcca cacctcggag gcgatgatga cgaacagcga cgtgccctgc gaggagaacc
    10321 agttgaggtc ctcggtgatg cccggcaccc agtcgaacca gttgttgacg tagccgccgg
    10381 agttgatgtc
//
