LOCUS       NZ_AP023440            10405 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces aurantiacus strain JCM 4677 chromosome.
ACCESSION   NZ_AP023440
VERSION     NZ_AP023440.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces aurantiacus
  ORGANISM  Streptomyces aurantiacus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 9428393)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye
            Genome Coverage                   :: 62X
            Sequencing Technology :: Illumina HiSeq X Ten; PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014701115.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 05:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,211
            CDSs (total)                      :: 8,122
            Genes (coding)                    :: 7,928
            CDSs (with protein)               :: 7,928
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 69 of 194
            Pseudo Genes (incomplete)         :: 161 of 194
            Pseudo Genes (internal stop)      :: 17 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:48:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7298127
            Orig. end                         :: 7308532
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023440.1.
            Genome sequencing and analysis were conducted under the Global
            Catalogue of Microorganisms (GCM) 10K type strain sequencing
            project (http://gctype.wdcm.org). The genomic data are also
            available at http://gctype.wdcm.org/sequencing/GCM10017557
            Annotated by DFAST v.1.2.7 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="25"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(401..418)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.09"
                     /tool="antismash"
     gene            434..1963
                     /locus_tag="IGS71_RS31300"
                     /old_locus_tag="GCM10017557_63870"
     CDS             434..1963
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187946.1"
                     /locus_tag="IGS71_RS31300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63870"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_190853353.1"
                     /sec_met_domain="Aldedh (E-value: 5.1e-43, bitscore: 146.1,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAAPVWSVDPRTGKQREQVAVEATAQEVDEAVRAAHAARGALAD
                     RTVRAAFLRTAADQLEDAKEPLVEAADAETALGPVRLTGELARTCYQLRAFADIVDEGE
                     FLGVVINHPDATATPPIPDLRRYKVPLGVVAVYSASNFPFAFSVAGGDTASALAAGCPV
                     VVKAHPDHPGLSELVASVLRRAAGLHGIPDGVLGLVHGFEAGVELVKHPLVSAAGFTGS
                     VRGGRALFDAAAARPAPIPFHGELGSLNPVVITEAAAAERAEAIGTGLAGSVTLGVGQF
                     CVKPGLVLAPAGAAGDRLLKSLTDAVSDTDAGVLLDHRMRDNFVAGVAERAGLPDVDAP
                     VTPGAGGEHTVSPGFLTVPAGRLATESEYDLLLEECFGPVTVVARYADEAEANAVLARL
                     PGNLTATVHLSAEEEAGGGRGAEILAELTPLAGRVVVNAWPTGVAVAPAQQHGGPYPAT
                     TSTSTSVGGTAIERWLRPVAYQNAAEGLLPAELRDGNPLGLPRRYDGRLER"
     gene            2109..2717
                     /locus_tag="IGS71_RS31305"
                     /old_locus_tag="GCM10017557_63880"
     CDS             2109..2717
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="IGS71_RS31305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63880"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_190853354.1"
                     /transl_table=11
                     /translation="MDLELPELPFSLRSYGPDGHWSYEGGVLTGWAGARQDRFVPPTDE
                     GLAPASDAPRLLGAPEGDFQLIARVTVGFAGAFDAGVLYIHVGERAWAKLCLENSPDVP
                     TVCTVVTRGHSDDANSFTVDGSSVWLRISRTGRAFAFHASKDGERWTFVRLFTLADEKE
                     TGAALVGFMTQSPMGEGCVVTYDHIAFRPDWPADLRDGS"
     misc_feature    complement(2827..2845)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.25"
                     /tool="antismash"
     misc_feature    2946..2961
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3114..3653
                     /gene="ectA"
                     /locus_tag="IGS71_RS31310"
                     /old_locus_tag="GCM10017557_63890"
     CDS             3114..3653
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002246.1"
                     /locus_tag="IGS71_RS31310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63890"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_055511172.1"
                     /transl_table=11
                     /translation="MTAAQADLQAEFLEMPEGLRIDRPQVVDGAALWRIAKDSGTLDLN
                     SSYSYLLWCRDFAGTSAVARTADGEPVGFVTGYLRPERPQALLVWQVAVDAAQRGRGLA
                     AALLDGLTLRVAGQSELTSVETTITPGNTASERLFTSYAARHGATVEREVLFDTGHFPD
                     GPHDPEVLYRIGPLSL"
     gene            3678..4949
                     /gene="ectB"
                     /locus_tag="IGS71_RS31315"
                     /old_locus_tag="GCM10017557_63900"
     CDS             3678..4949
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 9.9e-72.
                     Score: 233.7. Matches aSDomain:
                     nrpspksdomains_IGS71_RS31315_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="IGS71_RS31315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63900"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_055511171.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.2e-76, bitscore:
                     255.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRSWPTVFDRAQGSRMFDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRSFLESFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALAGLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALETYWSDGPAMEKQTRARGEQIEQSL
                     ISVTEENLADVKEYRGRGLVWGMEFHDKARAGRVAHRAFELGLLIETSGPEGEVVKLLP
                     ALTITPDELDEGLRTLARAVRETA"
     aSDomain        3762..4775
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IGS71_RS31315_Aminotran_3.1"
                     /evalue="9.90E-72"
                     /label="IGS71_RS31315_Aminotran_3.1"
                     /locus_tag="IGS71_RS31315"
                     /protein_end="366"
                     /protein_start="28"
                     /score="233.7"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRSFLESFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALAGLCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALETYWSDGPAMEKQTRARGEQIEQSLISVTEENLADVKEYRGRGLVWGMEFH"
     gene            5001..5405
                     /locus_tag="IGS71_RS31320"
                     /old_locus_tag="GCM10017557_63910"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="IGS71_RS31320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63910"
                     /product="ectoine synthase"
                     /protein_id="WP_190853356.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.6e-54, bitscore:
                     179.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEIEGTDRHVKSASGTWESKRIVLAKEKVGFSVHETILY
                     AGTETSMWYANHIEAVVCVAGEAELTDDETGKKYTITPGTMYLLDGHERHRLRVKENFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5410..6297
                     /gene="thpD"
                     /locus_tag="IGS71_RS31325"
                     /old_locus_tag="GCM10017557_63920"
     CDS             5410..6297
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="IGS71_RS31325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63920"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_190853358.1"
                     /sec_met_domain="PhyH (E-value: 5.5e-38, bitscore: 130.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVTDLYPSRGASEVSTPRQDPVVWSEPGTPGPIPGADLQSYER
                     DGFLSVGQLIDDDEVAVYRAELERLVTDPAVRADERSIVEPQSQEIRSVFEVHRTSEVF
                     ARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSIALTENFDTNGGLMIMPGSHHTFLGCSGATPKDNYKKSLQMQDAGTPSDEALTKY
                     ASQHGIKLFTGRAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPFAAPVR
                     RPEFIGARDFTPVK"
     gene            complement(6373..7434)
                     /locus_tag="IGS71_RS31330"
                     /old_locus_tag="GCM10017557_63930"
     CDS             complement(6373..7434)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947424.1"
                     /locus_tag="IGS71_RS31330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63930"
                     /product="alkene reductase"
                     /protein_id="WP_055511209.1"
                     /transl_table=11
                     /translation="MTTAHDPINLSGTPLTNRIVMAPMTRSRAGDGGAPTDLNAEYYAQ
                     RASAGLIVTEGIQPSAVGQGYPDTPGLHSAEQIAAWRKVTDAVHAAGGRIFAQLMHAGR
                     IGHPTLLPDGLVNVGASPVPAQGQVYTHEGPLDYITPRELTGDEVRATVGEFAAAAKGA
                     IEAGFDGVELHGANGYLIHQFLAPGSNHRTDEWGGSVENRIRFAVETVKAVVAEIGAGR
                     TGIRLSPGNPFNDIQEPEPEPTYTALVAALEPLGLAYLHITEVGEVRELTLALRKRFSG
                     TFILNPATEGPTGPESLALIEDGAADLVAFGRLFLANPDLPARLRAGGPFNEADAATLY
                     GGDAKGYTDYPAL"
     misc_feature    complement(7559..7574)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.33"
                     /tool="antismash"
     gene            7607..8161
                     /locus_tag="IGS71_RS31335"
                     /old_locus_tag="GCM10017557_63940"
     CDS             7607..8161
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643608.1"
                     /locus_tag="IGS71_RS31335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63940"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_190853360.1"
                     /transl_table=11
                     /translation="MSATHEAEAAQDDPACTAHLSSAACGGPVSHAISRVARLHRTTAG
                     RLLRGAGLYPGQELVMMFLWDAGAVRQAELIKAVELDPSTVTKMLQRLEQAGHVRRRPD
                     PRDGRAVLVEATEQSSGLLDDVTRTWAGLEEHTLAGLSPAERAQLMHLLARVEANLCTD
                     TADCPAPPSTEPSTGPSTDRA"
     gene            complement(8204..9253)
                     /locus_tag="IGS71_RS31340"
                     /old_locus_tag="GCM10017557_63950"
     CDS             complement(8204..9253)
                     /NRPS_PKS="Domain: Aminotran_5 (16-343). E-value: 1.2e-24.
                     Score: 78.9. Matches aSDomain:
                     nrpspksdomains_IGS71_RS31340_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477739.1"
                     /locus_tag="IGS71_RS31340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63950"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_190853362.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.5e-18, bitscore:
                     64.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFAPKNTYLNTASTGLLPARAVAAVRAGVESVAAG
                     QPQDMFADVEATRAAFARLLGVPDSRVAAGASVAVYTGLIAASLPAGAEVLTAEGDFTS
                     VVNPFHMRRDLKVRTVPLERIAESVRPGTALVAVSAAQSADGRIADLPALREAAREHGA
                     RTYIDASQAAGWLAVDADAYDYVSSVAFKWLVCPRGVAFLVVPEDFGGLDPVFAGWVAG
                     ENPWDSCYGPVEELAHSARRFDESPSLFSYAGARHSLELMEELGVDTVRAHDLGLADRF
                     RAGLAALGQEPVPAPGSAIVSVPGLGHRQPELSRAGIEVSDRAGNLRAAFHLYNTPADV
                     DRLLDVLSG"
     aSDomain        complement(8225..9205)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IGS71_RS31340_Aminotran_5.1"
                     /evalue="1.20E-24"
                     /label="IGS71_RS31340_Aminotran_5.1"
                     /locus_tag="IGS71_RS31340"
                     /protein_end="343"
                     /protein_start="16"
                     /score="78.9"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARAVAAVRAGVESVAAGQPQDMFADVEATRAAF
                     ARLLGVPDSRVAAGASVAVYTGLIAASLPAGAEVLTAEGDFTSVVNPFHMRRDLKVRTV
                     PLERIAESVRPGTALVAVSAAQSADGRIADLPALREAAREHGARTYIDASQAAGWLAVD
                     ADAYDYVSSVAFKWLVCPRGVAFLVVPEDFGGLDPVFAGWVAGENPWDSCYGPVEELAH
                     SARRFDESPSLFSYAGARHSLELMEELGVDTVRAHDLGLADRFRAGLAALGQEPVPAPG
                     SAIVSVPGLGHRQPELSRAGIEVSDRAGNLRAAFHLYNTPADVDRL"
     gene            complement(9349..10065)
                     /locus_tag="IGS71_RS31345"
                     /old_locus_tag="GCM10017557_63960"
     CDS             complement(9349..10065)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140791.1"
                     /locus_tag="IGS71_RS31345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_63960"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_190853364.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALDAFPHRDGVEVVHRSFELDP
                     GRAKGDIQAVIPMLTEKYGMSEAQAQAGEENLGTLAAAEGLAYRAQGRDTGNTFDMHRL
                     LHLAKERGRQDELMQAFYEANFTEERSVFGDDERLVELAAGAGLGEDEVRKVLADPAAY
                     ADDVRADEREAAELGANGVPFFVLDRKYGVSGAQPAEVFTQALEQAWASHSPLKLIQQD
                     DAEACGPDGCAVPQQH"
ORIGIN
        1 gagagccggt ccagcgtggg ccgtgccgcg gccacgacct cgtcgccgtc gatgtacgac
       61 gtgccgacga gcagtgcccg tacgccgatg ccgtaccgcg tgcccgtcgc gtcggtctcg
      121 acccagccga gttcgacaag cgtgcgcagc aacatgtaaa gactggactt ggggtacccg
      181 acggcttcct gaacaccggc cagggagtgc ataccggggc gtccggcgaa gtactcaagc
      241 aattcaaccg tccgcaccgc ggacttgacc tgcgctccgc cgcccgtctc gcctgccgac
      301 atcggccttg accccctagt tcgccgggaa atagtctcga cagcatattc accaccagag
      361 acagcgttca gcatatcgaa cgccttcggt gagtggcaca tgaactgcgg cattacatct
      421 ggagggaccc gcggtggcag cagcaccagt ctggagtgtc gacccccgaa ccgggaagca
      481 gcgcgagcag gttgcggtgg aagccacagc ccaggaggtg gacgaggccg tccgcgccgc
      541 gcacgccgcc cggggggctc tcgccgaccg cacggtccgc gcggccttcc tgcgcaccgc
      601 ggccgaccag ctcgaagacg ccaaggaacc cctcgtggag gcggccgacg cggagaccgc
      661 gctcggcccg gtccggctca ccggcgaact cgcccgtacc tgctaccagc tgcgggcctt
      721 cgcggacatc gtcgacgagg gcgagttcct cggcgtcgtg atcaaccacc ccgacgccac
      781 ggcgaccccg ccgatcccgg acctgcgccg ttacaaggtg ccgctgggcg tcgtcgccgt
      841 ctactcggcc tcgaacttcc ccttcgcctt ctcggtggcc ggcggcgaca ccgccagcgc
      901 gctcgccgcg ggctgcccgg tcgtcgtcaa ggcgcatccc gaccacccgg gcctgtccga
      961 gctggtcgcc tccgtcctgc gtcgggccgc cggcctgcac ggcatcccgg acggcgtcct
     1021 cggcctggtc cacggcttcg aggcgggcgt cgagctggtc aagcacccgc tggtctcggc
     1081 cgccggtttc accggctcgg tacgcggcgg acgcgccctc ttcgacgcgg cggcggcgcg
     1141 gcccgcgccg atcccgttcc acggcgagct gggctcgctc aacccggtcg tgatcaccga
     1201 ggccgcggcg gccgagcgcg ccgaggcgat cggtacgggg ctcgcgggct ccgtgacgct
     1261 gggcgtcggc cagttctgtg tgaagccggg cctggtgctg gcgccggccg gcgcggcggg
     1321 ggaccgcctg ctgaagtccc tgacggacgc ggtgagcgac accgacgcgg gcgtcctgct
     1381 cgaccaccgc atgcgcgaca acttcgtcgc gggggtcgcc gagcgcgccg ggctgcccga
     1441 cgtggacgcc ccggtgacgc cgggagcggg cggtgagcac acggtgagcc ccgggttcct
     1501 gacggtgccc gcggggcggc tggccaccga gagcgagtac gacctgctgc tcgaagagtg
     1561 cttcggtcct gtgaccgtgg tcgcgcgcta cgcggacgag gccgaggcga acgcggtgct
     1621 cgcgcggctg ccgggcaacc tcaccgcgac ggtccacctg tcggcggagg aggaggccgg
     1681 cggggggcgt ggcgcggaga tcctcgccga gctcacgccg ctcgcggggc gcgtggtcgt
     1741 gaacgcgtgg ccgacggggg tcgccgtggc gccggcccag cagcacggcg ggccgtatcc
     1801 cgcgacgacg tccacgtcca cctccgtcgg cgggacggcc atcgagcggt ggctgcggcc
     1861 ggtcgcgtac cagaacgcgg cggaggggtt gttgccggcc gagttgcggg acgggaatcc
     1921 gttggggttg cctcggcggt atgacgggcg cctggagcgg tagcgctccg ctttttgtgc
     1981 ggttgggtcc gtggccgtca gtagtcggct gcgggcccgt tgtggcttgt cgcgcagttc
     2041 cccgcgcccc tgaagacaga agatcgcgca gttccccgcg cccctgacgg ggctgggatg
     2101 atccgggtat ggatctcgaa cttcccgaac ttccgttttc tcttcgtagt tatgggcccg
     2161 acgggcactg gtcgtacgag ggtggggtgc tcaccgggtg ggcgggggcg cggcaggacc
     2221 ggttcgtgcc gcccaccgat gaggggctgg cccccgcgtc cgacgcgccc cggctgctgg
     2281 gggcgcccga aggggacttc cagctgatcg ctcgcgtcac ggtcggcttc gccggggcct
     2341 tcgacgcggg tgtgctctac atacacgtgg gcgaacgggc ctgggcgaag ctctgcctcg
     2401 aaaactcccc ggacgtgccc accgtctgca cggtcgtcac cagagggcac tcggacgacg
     2461 ccaactcctt cacggtcgac ggcagctccg tctggctccg gatcagccgc accggccggg
     2521 ccttcgcctt ccacgcctcc aaggacggtg agcgctggac cttcgtccgc ctcttcaccc
     2581 tggcggacga gaaggagacc ggcgcggccc tggtcggctt catgacccag tccccgatgg
     2641 gcgagggctg cgtggtcacg tacgaccaca tcgcgttccg cccggactgg ccggcggacc
     2701 tgcgggacgg ctcctgaacc tcgcgcacgg cggcgctcca gcggggcgcc gccacggccg
     2761 tgggcggctg tcggcgtcgg gtcgcggagt cgcggaaatt ctgatctgat ttaatcaggc
     2821 actcatactg tctttattta ttgcagcttc ccataagctt ctgcccgctt tcttgggcct
     2881 taaacgccac gatttttcga acatcggcct cgtgctacaa gcggatctcg tggcggtttc
     2941 gccgctgtga cgcaaaccac agactgtctg ttctgtccga tatttcccgg acaaagtggc
     3001 ggtttcaacc aggccgacga ggggcccgcg cccgcgattt aacacacggc ccccgtttgc
     3061 gtaaccccat ccagcgatgc gatttcgact tttgctgggt aaatttaata gacatgaccg
     3121 ccgcacaagc agacctgcaa gcggaattcc tggaaatgcc agaagggctg cggatcgacc
     3181 gtccgcaggt ggtggacgga gccgcactct ggcgcattgc caaggactcc ggaaccctcg
     3241 acctgaactc ctcctacagc tacctgctgt ggtgccgtga cttcgccggc acctcagcgg
     3301 tggcgcgtac ggccgacggc gagcccgtcg gcttcgtcac cggttacctc cggcccgagc
     3361 gcccccaggc gcttctcgtg tggcaggtgg ccgtcgacgc ggcacagcgt ggacgtggtc
     3421 tcgccgcggc gctcctcgac gggctgaccc tccgggtcgc cggccagagc gagctgacct
     3481 ccgtggagac caccatcacc ccgggcaaca ccgcatccga gcggctgttc acttcgtatg
     3541 ccgcgcgcca cggcgcgacc gtcgagcggg aggtcctgtt cgacacaggg cacttccccg
     3601 acggcccgca cgaccccgaa gtgctgtacc gcatcggtcc cctgtcgctc tgacccccac
     3661 gcctgaggag tatcgccgtg accatcaccc agcccgacct gagcgtcttc gaaaccctgg
     3721 agtcggaggt gcgcagctac tgccgcagct ggcccaccgt cttcgaccgc gcgcagggca
     3781 gccgcatgtt cgacgaggac ggccatgaat acctcgactt cttcgcgggc gccggctcac
     3841 tcaactacgg acacaacaac cccgtactga aacgcgccct gatcgactac ctggaacgcg
     3901 acggcgtcac ccacggtctc gacatgtcga cgaccgccaa gcgcagcttc ctggagtcgt
     3961 tccagaacct ggtgctgcgc ccccgcgacc tgccgtacaa ggtcatgttc cccggcccga
     4021 cgggaaccaa cgccgtcgag tccgcgctga agctggcccg caaggtgaag gggagggagt
     4081 ccatcgtctc cttcaccaac gccttccacg gcatgtcgct cggctcgctc gccgtgaccg
     4141 gcaacgcctt caagcgggcc ggcgccggta tcccgctcgt gcacggcacg ccgatgccct
     4201 tcgacaacta cttcgacggg caggtcccgg acttcctctg gttcgagcgg ctcctggagg
     4261 accagggctc aggcctcaac aagcccgccg ccgtgatcgt cgagacggtg cagggcgagg
     4321 gcggcatcaa cgtcgcccgt gccgagtggc tgcgcgccct cgccggcctg tgcgagcgcc
     4381 aggacatgct gctgatcgtc gacgacatcc agatgggctg cggccgcacg ggcgccttct
     4441 tctccttcga ggaggcgggg atcgtgcccg acatcgtgac cgtctccaag tccatcagcg
     4501 gctacgggct gcccatgtcc ctgtgcctgt tcaagcccga gctggacatc tgggagccgg
     4561 gcgagcacaa cggcacgttc cgcggaaaca accccgcctt cgtgacggcc gccgccgccc
     4621 tggagacgta ctggtccgac gggcccgcca tggagaagca gacccgcgcc cgcggcgagc
     4681 agatcgagca gtcgctgatc tccgtcaccg aggagaacct cgccgacgtg aaggagtacc
     4741 gcggccgcgg cctggtctgg ggcatggagt tccacgacaa ggcgcgcgcg ggccgcgtgg
     4801 cccaccgggc cttcgaactc ggcctgctga tcgagacgtc gggcccggag ggcgaggtcg
     4861 tgaaactcct cccggcgctc accatcacgc ccgacgagct ggacgagggc cttcgtaccc
     4921 tcgcccgtgc cgtccgagag acggcctgaa ccgcaccccc ccgcagagca agaacagcct
     4981 gaaggaggct tcgtagcacc gtgatagtcc gttcgttcaa ggagatcgaa ggcaccgacc
     5041 gacacgtgaa atcggcgtcg ggcacctggg agagcaagcg catcgtcctc gccaaggaga
     5101 aggtcggctt ctccgtgcac gagacgatcc tgtacgcggg cacggagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtcgtctgcg tggcgggtga ggccgagctc accgacgacg
     5221 agaccggcaa gaagtacacg atcacgccgg gaaccatgta cctcctcgac gggcacgaga
     5281 ggcaccggct gcgggtcaag gagaacttcc gctgcatctg tgtcttcaat ccgccggtga
     5341 ccggacggga ggaccacgac gagaacggcg tctaccccct gctgaccgag cccgaggagg
     5401 tctgaaccga tgacgaccgt caccgacctg tatcccagcc gcggcgccag tgaggtgtcc
     5461 acgccccgac aggacccggt tgtgtggagc gagcccggta cgccgggccc catcccgggc
     5521 gccgacctgc agtcgtacga gcgggacggc ttcctctccg tcgggcagct catcgacgac
     5581 gacgaggtcg ccgtctaccg ggccgagctg gagcgcctgg tcaccgaccc ggcggtccgc
     5641 gccgacgagc gctcgatcgt cgagccgcag tcgcaggaga tccgctcggt cttcgaggtc
     5701 caccgcacca gcgaggtctt cgcccggctg gtgcgcgacg agcgtgtggt gggccgcgcc
     5761 cggcagatcc tcggctccga cgtgtacgtg catcagtcac ggatcaacgt caagcccggt
     5821 ttcggcgcca gcggcttcta ctggcactcg gacttcgaga cctggcacgc cgaggacggc
     5881 ctgccgaaca tgcggaccgt gtccgtctcg atcgcgctga ccgagaactt cgacaccaac
     5941 ggcggcctca tgatcatgcc ggggtcgcac cacacgttcc tcggctgctc gggggccacg
     6001 ccgaaggaca actacaagaa gtcgctgcag atgcaggacg cgggcacgcc gtccgacgag
     6061 gcgctcacca agtacgcgtc ccagcacggc atcaagctct tcaccggccg ggccggctcg
     6121 gcgacctggt tcgactgcaa ctgcatgcac ggctcgggcg acaacatcac gccgttcccg
     6181 cgctcgaacg tcttcatcgt gttcaacagc gtggagaacg cggcggtgga gccgttcgcg
     6241 gcaccggtgc gccggcccga gttcatcggg gcgagagact tcactccggt gaagtgacgt
     6301 ccgcccgaag gcgatcggcc cggcgcccga ctcacgcgag tcgggcgccg ggccgagtcc
     6361 gatccaactg ccttacagcg ccgggtagtc cgtgtagccc ttcgcgtccc cgccgtagag
     6421 cgtcgccgcg tcggcctcgt tgaacggacc gccggccctc agccgggcgg gcaggtcggg
     6481 attggccagg aacagccggc cgaaggcgac gagatcggcg gcgccgtcct cgatgagcgc
     6541 cagggactcc ggcccggtcg gcccctcggt cgccgggttc aggatgaagg tgccgctgaa
     6601 ccgcttgcgc agcgcgagcg tcagctcacg gacctcgccc acctcggtga tgtgcagata
     6661 cgcgaggccc agcggctcca gcgcggccac gagggcggtg tacgtcggct ccggctcggg
     6721 ctcctggatg tcgttgaacg ggttgccggg tgagagccgg atccccgtac gaccggcgcc
     6781 gatctccgcg acgacggcct tgacggtctc gaccgcgaac cggatgcggt tctcgaccga
     6841 gccgccccac tcgtcggtgc ggtggttgga gcccggggcg aggaactggt ggatcagata
     6901 gccgttggcg ccgtgcagtt cgaccccgtc gaagcccgcc tcgatcgcgc cctttgcggc
     6961 tgccgcgaac tcgcccacgg tggcgcggac ctcgtcgccg gtcagctcgc gcggggtgat
     7021 gtagtcgagg gggccctcgt gggtgtagac ctgcccctgg gccggcacgg gcgacgcgcc
     7081 gacgttgacg agcccgtcgg gcagcagcgt cgggtgaccg atccggcccg cgtgcatgag
     7141 ctgcgcgaag atccggccgc ccgcggcgtg cacggcgtcg gtcaccttcc gccaggccgc
     7201 gatctgctcg gcactgtgca ggcccggcgt gtccggatag ccctggccca ccgcggacgg
     7261 ctggatgccc tcggtgacga tcaggccggc ggacgcgcgc tgggcgtagt actcggcatt
     7321 gagatcggtc ggcgctccgc cgtcgccggc ccggctgcgc gtcatcggcg ccatcacgat
     7381 gcggttcgtg agcggcgtgc cggacaggtt gatcgggtcg tgtgcggtgg tcaccatggc
     7441 tcctcggagg gtatgtgtgg tcggccaact acccgaacgg gatgcccccg cgattaattc
     7501 cttggtcggc caaaatgatg tgtcgcctcc cgaggtaatc tcggttgggt gcttggctga
     7561 gacggacatg tgacgtgcgc gggaaccgcg ggagcaagga gtcgggatga gtgccaccca
     7621 cgaggccgag gccgcacagg acgaccccgc ctgtaccgcc cacctgtcct ccgcggcgtg
     7681 cggcggcccg gtcagtcatg ccatctccag ggtggcccgg ctgcaccgca ccacggcggg
     7741 caggctgctg cgcggggccg ggctctaccc cggtcaggag ctcgtgatga tgttcctgtg
     7801 ggacgcgggc gcggtgcgtc aggcggagct gatcaaggcg gtcgagcttg atccgtcgac
     7861 ggtcacgaag atgctccagc gcctggaaca ggcaggacac gtacgccgcc gccccgaccc
     7921 gagggacggc cgcgctgtcc tcgtcgaggc gacggagcag agcagcgggc tcctcgacga
     7981 cgtcacgcgc acctgggccg gactggagga gcacaccctc gccgggctga gccccgccga
     8041 gcgcgcccag ctcatgcacc tcctcgcgcg cgtcgaggcg aacctgtgca cggacacggc
     8101 cgactgcccg gccccgccga gcaccgagcc gagcaccggg ccgagtaccg accgggccta
     8161 gagccaggcc ggcgccgccg cccgttcgtg gtccgcgcgg cagtcagccg gacaggacgt
     8221 ccagcaggcg atcgacgtca gcgggcgtgt tgtacagatg gaaggcggcc cgcagattgc
     8281 ccgcgcggtc ggagacctcg atgcccgccc ggctcagctc cggctgccgg tgaccgagtc
     8341 cgggcacgga gacgatggcc gagcccggcg ccgggaccgg ctcctgcccc agagcggcga
     8401 ggcccgctcg gaaccggtcg gcgaggccca ggtcgtgggc gcgtacggtg tccacgccca
     8461 gctcctccat cagttcgagg gagtggcggg caccggcgta cgagaagagg ctggggcttt
     8521 cgtcgaaccg ccgtgcgctg tgggcgagtt cctcgaccgg cccgtagcag ctgtcccagg
     8581 ggttctctcc cgcgacccag cccgcgaaga ccgggtcgag ccctccgaag tcctccggaa
     8641 cgaccaggaa ggccacgccc cgcggacaga ccagccactt gaaggccacg gaggagacgt
     8701 agtcgtacgc gtccgcgtcg accgccagcc atccggcggc ctgcgacgcg tcgatgtacg
     8761 tacgggcgcc gtgttcgcgc gcggcctcgc gcagcgccgg caggtcggcg atccggccgt
     8821 ccgcggactg tgccgcgctc accgcgacga gcgcggtgcc ggggcgcacc gactcggcga
     8881 tccgctccag cggcaccgta cgcaccttga ggtcgcggcg catatggaag gggttcacca
     8941 cggaggtgaa gtcgccctcg gcggtgagga cttcggcacc cgcgggcagc gaggcggcga
     9001 tcagtccggt gtagacggcg accgaggccc cggccgccac acggctgtcc gggaccccga
     9061 ggagccgggc gaaggcggcg cgggtggcct ccacgtcggc gaacatgtcc tgcggttgtc
     9121 cggcagccac ggactcgacg ccggcccgca ccgcggcgac cgcgcgggcc gggagcagcc
     9181 cggtactggc ggtgttcagg taggtgttct tcggggcgaa ctcggcacgg acgaggctct
     9241 cgaaggtctc catggaaacc actgtgcggc cccggggacc tgtcgtccat tgccgatttt
     9301 tacgtggttt cccttaggaa cgcttatagg tccgcctcga cctgcgcttc agtgctgctg
     9361 gggcaccgcg cagccgtccg ggccgcacgc ctcggcgtcg tcctgctgga tcagcttgag
     9421 cggggagtgg ctcgcccacg cctgctccag ggcctgcgtg aagacctcgg cgggctgggc
     9481 gccggagacg ccgtacttgc ggtccaggac gaagaacggc acgccgttcg cacccagctc
     9541 ggcggcctcg cgctcgtcgg cgcggacgtc gtcggcgtac gcggccgggt cggcgagcac
     9601 cttgcggacc tcgtcctcgc cgagcccggc gccggccgcc agttccacga gccgctcgtc
     9661 gtcgccgaag acggaacgct cctcggtgaa gttcgcctcg tagaaggcct gcatcagctc
     9721 gtcctggcgg ccccgctcct tggcgaggtg cagcagccgg tgcatgtcga aggtgttgcc
     9781 ggtgtcgcgg ccctgggcgc ggtaggccag gccctccgcg gcggccagcg tgccgaggtt
     9841 ctcctcgccg gcctgagcct gcgcctcgct catgccgtac ttctcggtga gcatcgggat
     9901 gaccgcctgg atgtcgccct tcgcgcgacc ggggtcgagc tcgaaggaac ggtgcaccac
     9961 ctcgacgccg tcgcggtgcg ggaaggcgtc gagcgccttc tcgaagcggg ccttgcccac
    10021 gtagcaccag gggcaggcga tgtcgctcca gatctcgacg cgcatgtctc cgactctttc
    10081 ccgctcgaac ggacagcggg tgcgtcccgc tggatgcgtg aacgttcaat ggctgacgtt
    10141 cattcccggc tgcgggcccg gtgggggttg ctcgcgcagt tccccgcgcc ccttaaaagc
    10201 agggctgcgc cccagcaaac caaggggcgc ggggaactgc gcgccgagcc cccaccggcc
    10261 cgcaggttct gcttttcagg ggcgcgggga actgcgcgcc gagcccccac cgggccgcag
    10321 gtggaactcg gtgctcaggc cggtgctcag gccggtgctc aggcggccag cgccgcgccc
    10381 agcgcggtga ggcaccgccc gacca
//
