LOCUS       NZ_AP023440            10471 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces aurantiacus strain JCM 4677 chromosome.
ACCESSION   NZ_AP023440
VERSION     NZ_AP023440.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces aurantiacus
  ORGANISM  Streptomyces aurantiacus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 9428393)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye
            Genome Coverage                   :: 62X
            Sequencing Technology :: Illumina HiSeq X Ten; PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014701115.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 05:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,211
            CDSs (total)                      :: 8,122
            Genes (coding)                    :: 7,928
            CDSs (with protein)               :: 7,928
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 69 of 194
            Pseudo Genes (incomplete)         :: 161 of 194
            Pseudo Genes (internal stop)      :: 17 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:48:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6191876
            Orig. end                         :: 6202347
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023440.1.
            Genome sequencing and analysis were conducted under the Global
            Catalogue of Microorganisms (GCM) 10K type strain sequencing
            project (http://gctype.wdcm.org). The genomic data are also
            available at http://gctype.wdcm.org/sequencing/GCM10017557
            Annotated by DFAST v.1.2.7 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10471
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="22"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10471
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10471
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5471](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5471
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            1618..1857
                     /gene="chpG"
                     /locus_tag="IGS71_RS26410"
                     /old_locus_tag="GCM10017557_54000"
     CDS             1618..1857
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="IGS71_RS26410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54000"
                     /product="chaplin ChpG"
                     /protein_id="WP_055513340.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSVAAAAMAGTAGIASADSGAQGAAAHSPGVLSGN
                     VVQVPVHVPVNLCGNTIDIIALLNPAFGNECEND"
     misc_feature    complement(2025..2039)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     gene            2144..2911
                     /locus_tag="IGS71_RS26415"
                     /old_locus_tag="GCM10017557_54010"
     CDS             2144..2911
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644660.1"
                     /locus_tag="IGS71_RS26415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54010"
                     /product="hypothetical protein"
                     /protein_id="WP_190852452.1"
                     /transl_table=11
                     /translation="MHALPVRRIATSALCATFLLGMAGPVAVAADHDSAHATRATTAQA
                     PVPDAEALLAQTKGLGDIADVVKPVTDLLEDVLKADNGQLPAADATKLADAVKEAVAKA
                     TAAAPASPQTPPSTTPTEPKAPNQPDTPNQPDTPNTPNTPNTPNTPVDPPAVLPALPDT
                     SAVPQTDGRVAAPDVRADALKALQNSVDSLVKAATGGDTTAVKAASTTVVSGLVNVVAA
                     ISVGGGLPAANLEGLSKQPTDAAQPSASGLLPS"
     gene            3224..3520
                     /locus_tag="IGS71_RS26420"
                     /old_locus_tag="GCM10017557_54020"
     CDS             3224..3520
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672804.1"
                     /locus_tag="IGS71_RS26420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54020"
                     /product="hypothetical protein"
                     /protein_id="WP_055513344.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSMVVAGVAAPAFATDVMPPSSLNGGLESAVTQL
                     TTEPVDVMPLQSQSRALDTENEDSALSTVTAATETLNSASGPTGLLGGLPLQK"
     gene            3697..4212
                     /locus_tag="IGS71_RS26425"
                     /old_locus_tag="GCM10017557_54030"
     CDS             3697..4212
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065676.1"
                     /locus_tag="IGS71_RS26425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54030"
                     /product="DUF5949 family protein"
                     /protein_id="WP_190852453.1"
                     /transl_table=11
                     /translation="MTSTSISTSPSSGTHPSSAANLGTLVVMAWSGAHPDGDMPYLLAY
                     SLGDGQGGPEGSAVAVEQLLSANGLPLGDTVVDATRQPSFPVSLLVEAGQAVVSMTELK
                     ARCPVPPEWLAAVGERGYAYLLFTTRPWPEAEPGKPVEPAALAAFAGDEETLKNAAHAL
                     VPAGSPCG"
     misc_feature    3703..3717
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.6"
                     /tool="antismash"
     misc_feature    4302..4322
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.29"
                     /tool="antismash"
     gene            4416..4925
                     /locus_tag="IGS71_RS26430"
                     /old_locus_tag="GCM10017557_54040"
     CDS             4416..4925
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="IGS71_RS26430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54040"
                     /product="hypothetical protein"
                     /protein_id="WP_055513348.1"
                     /transl_table=11
                     /translation="MKSTTRGTFAAVVTCVAAAVATPAVAADGIPVAVPLEGAEHALDM
                     ELPSVSGTVPLLKPGSPDGPRYVEGRLLPDRALPQLPVTSDLPSADLRTPVPRVLGDTF
                     DHLAVGAPTSSGLRARTPGASLDGPLTAPRPDRLGLPAPKLPEAGLLAPVLQAAPGATL
                     GLAPGL"
     gene            5001..5471
                     /locus_tag="IGS71_RS26435"
                     /old_locus_tag="GCM10017557_54050"
     CDS             5001..5471
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003508.1"
                     /locus_tag="IGS71_RS26435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54050"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_190855643.1"
                     /sec_met_domain="melC (E-value: 8.3e-35, bitscore: 118.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVALTPLVTASRRARAEPYTAGTPIGFEAESEAESESESESEAR
                     FAAGFDEMYGGSRIRGFKDTAGVCSDGVARAATAGPWRVTVDDRPLHLMRRADGGYLTM
                     LDHYQSYPTPLAAARAAVDELDGTLRLREATTGAEQGEGHGGPGGYGDGVPA"
     gene            5455..6321
                     /locus_tag="IGS71_RS26440"
                     /old_locus_tag="GCM10017557_54060"
     CDS             5455..6321
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140533.1"
                     /locus_tag="IGS71_RS26440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54060"
                     /product="tyrosinase family protein"
                     /protein_id="WP_190852455.1"
                     /transl_table=11
                     /translation="MAYPRKNVSALTSTEKRRLVDAFLEIKRTGEYDEFVRMHIDHYVS
                     DGEGGLRAAHMAPSFLPWHRKFLLDLERALRRTDSGVSVPYWDWTKDRTPAAPPWGEDL
                     LGGNGRRSDHQVTTGPFARRHKKWVVKESMTDGDYLTRDFGRPRDPLALPTADDLAKAL
                     ADPVYDTEPWDSTSGRGFRNGLEGWGTGRGNDAWRNHNRVHRWVGGHMLGGGSVNDPVF
                     WLHHSFVDLLWTRWQKRHQGARYLPKSPPGLGDRQRGRIVARHEAMPPWGVTPDEMEDH
                     SRIYRYV"
     gene            complement(6441..6707)
                     /locus_tag="IGS71_RS26445"
                     /old_locus_tag="GCM10017557_54070"
     CDS             complement(6441..6707)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642287.1"
                     /locus_tag="IGS71_RS26445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54070"
                     /product="chaplin"
                     /protein_id="WP_055513353.1"
                     /transl_table=11
                     /translation="MSRIAKAAGVALGTGAVVLSGASLAMADADAAGKAVGSPGVASGN
                     VIQVPLHIPVNLCGNTVDIIALLNPTFGNECANIDDTPHGYGH"
     gene            complement(6877..7593)
                     /locus_tag="IGS71_RS26450"
                     /old_locus_tag="GCM10017557_54080"
     CDS             complement(6877..7593)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573669.1"
                     /locus_tag="IGS71_RS26450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54080"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_055513414.1"
                     /transl_table=11
                     /translation="MNDRTALVTGANKGIGKHIAAQLAAEGFTVYVGSRDVGRGQRAAE
                     EIVGDARPLVLDVTDPRSVAEAAAQVDRLDVLVNNAGISVSLTPPTEAGVEEFRRTYET
                     NVFAVVEVTNAFLPALRRSPRPRIVNVSSGTGSLTWSTNPNPQFAPGNGAAAAYRSSKT
                     ALNALTVFYAQTLAEEGFKVNALAPGLRATDLNTRAAASGGDPAEAAQEAVRLALLPDE
                     GPTGAFFSWDGTSVPW"
     gene            7670..8551
                     /locus_tag="IGS71_RS26455"
                     /old_locus_tag="GCM10017557_54090"
     CDS             7670..8551
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573668.1"
                     /locus_tag="IGS71_RS26455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54090"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_079103560.1"
                     /transl_table=11
                     /translation="MAREELARFLRDRREGLCPHELGLPTSTPRRTPGLRREEVAELAH
                     MSVDYYTRLEQARGPRPSPRILDALAQALRLVPAERSHLFRLAGSSLPPGTHAVQRVRP
                     QVAGMLRRLPETGAIVTDAAYGVVAWNPLAQALLGDDLGRGTTNLARRRFLGQGRSYES
                     SSAERFGHIVVARLRRAADRYPHDARLAGLLSELRSGSEEFRQIWETRPVHAPGHRTKV
                     LDHPTAGRLRLNCDVLLVPEDDQEVVLMTADPGSPSARTLRGLAARLDRVAVDAAELGS
                     CEQEPVPHRRRR"
     gene            complement(8556..9389)
                     /locus_tag="IGS71_RS26460"
                     /old_locus_tag="GCM10017557_54100"
     CDS             complement(8556..9389)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152052.1"
                     /locus_tag="IGS71_RS26460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54100"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_055513355.1"
                     /transl_table=11
                     /translation="MTADQDANRLGAYLRARRGLVTPEQAGIPAGGIRRVPGLRREELA
                     ILAGISADYYLRLERGRDTHPSAQVLDALARVLRLDEVEAEYLHSLATPRPRQRDRDRQ
                     RSRRRRAAEHVPARLHQLLAAVGVPAFVEGRAFDVLAANELATAFSPRLRPGENRLRSL
                     LLDPEEQSFHADWTASSEWFIAALRESIGDDVDNPRFVELIGELSLTSGRFRELWARHD
                     VRALEGGTTTVNHPVVGRLHLHRDKLPVNGLLLVLYYADAGSDSAEKLSLLASLV"
     gene            complement(9399..10193)
                     /locus_tag="IGS71_RS26465"
                     /old_locus_tag="GCM10017557_54110"
     CDS             complement(9399..10193)
                     /NRPS_PKS="Domain: PKS_KR (6-172). E-value: 5.2e-09. Score:
                     28.2. Matches aSDomain:
                     nrpspksdomains_IGS71_RS26465_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978630.1"
                     /locus_tag="IGS71_RS26465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_54110"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_190852457.1"
                     /transl_table=11
                     /translation="MDITSRTVFIAGATSGIGLELARRFRAAGSTVVVGGRRTDTFTRL
                     ADEGLVPVEIDVTDAGSVIRARDEVLRTHPGLDTVITMAGTGIPEDLRDPTHFGAAETT
                     VAVNVLGTIRVIDAFTPHLLARGNGGNSNSTIITVSSGIGFLPFPLMPTYGASKAAVHA
                     YTEALRAQLAGTGVEVAELVPPAVATPLGSGNPHALALDAYGAEVMDLLAQDPTPPEIL
                     VQGVLMHRWAERDGTYAELVAQRSQALSMLPGRSKSSGRSGS"
     aSDomain        complement(9678..10175)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IGS71_RS26465_PKS_KR.1"
                     /evalue="5.20E-09"
                     /label="IGS71_RS26465_PKS_KR.1"
                     /locus_tag="IGS71_RS26465"
                     /protein_end="172"
                     /protein_start="6"
                     /score="28.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TVFIAGATSGIGLELARRFRAAGSTVVVGGRRTDTFTRLADEGLV
                     PVEIDVTDAGSVIRARDEVLRTHPGLDTVITMAGTGIPEDLRDPTHFGAAETTVAVNVL
                     GTIRVIDAFTPHLLARGNGGNSNSTIITVSSGIGFLPFPLMPTYGASKAAVHAYTEALR
                     AQL"
     CDS_motif       complement(10113..10175)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IGS71_RS26465_0001"
                     /evalue="1.40E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="IGS71_RS26465"
                     /protein_end="27"
                     /protein_start="6"
                     /score="11.3"
                     /tool="antismash"
                     /translation="TVFIAGATSGIGLELARRFRA"
ORIGIN
        1 gcagggggtc gaggagaagc tcggtgacgg ccgcggccag cgcgtccggc cgctcgggcg
       61 gcaccagggc gcgccgccgg cccggcggca ggctttcgcg cgccccgtcc acatcggtga
      121 tcacgacggg ccgcccgcag gccatggcct ccagcggcgc cagcgccatg ccctcccaac
      181 gggacggcag gacgaccaga tcggcggcct ggtaccaggc cgccgagtcg gtgacggccc
      241 cggcgaacag cacggacgcg ggcgcccggg cccgcagcgc ccgcgcgtcc ggcccgtcgc
      301 cgaccagtac gaggcgcgcg tcgggcacgc gccgggtcac ctcacgccac gcctccagga
      361 ggacgtcctg tcccttctgc cggcacagcc gcccgacgca gaccgcgagc ggcgccgccg
      421 gatcgacccc ttcgagcagt gcgatcccgg cccgtacggt gtccaccggc gccgggtgga
      481 atccctccag gtcgatgccg ttggggatga cgctccagcg ccccctgatc cccgaccgct
      541 gcccggtccg ccgctcggcc tcgctgacac acacgacccg ggacgcccaa cgcacgccga
      601 atcgctccca gttgagcgcg agcagtccgg tggctccgcc gaccgcccgg aacgaccagg
      661 cgtgcggctg gaacacggtc ggcacccgcc cgcgcacggc cagccgtgcc gccagtccgg
      721 ccttggcgct gtgcgcgtgc accacgtcgg ggcgcacctc ccgtacgacc gcacgcagac
      781 tccgtacctc ctgggcgagc cacggacccg gggagcgggt cgcgggccag tgccgtacgg
      841 cggagcccag ggcctcggcc gccgccgcga ggccgccggt cgcagggcag gcgacggtga
      901 cttccgcgcc ggcggccagc tgagcccgca ccaggtcggc gaccacgcgg gccaccccgc
      961 cgtcgacggg ctgggagacg tgcaggactc gtggccgagg gccggtcggt gacagtcgca
     1021 tgcgcgcttt cctcgctcac gggtggcgct cgcagcgagc gcggtggaac tcggacagtc
     1081 gaagcagaac agccagggga gagcggccag gggagagcgg ccaggaggcg gaacctcgtt
     1141 ggcgactgtc aggtccgcgt gggtggcggg gcggtggcgg gggcagggag agccgatgcc
     1201 ggtccgcccg gcgcccggag cgaggcgagg gaaaaggcac ccaccattcc gcggcgcggc
     1261 agaagaccca gggaattgcg catgaccggc gttgcccttt cgggggaatt tcgatgggag
     1321 aggtactgac ccatacgggt ggacgagttg tggacatcac gtgattgccg tcatgactcc
     1381 ccgtctgttt caagcgatcc gaggagaaac gcaactgtag ccgctatccg tatttatcgg
     1441 tgaaacccgt caagtgtgtc ggaatcgtgt gcatgccccg ggacaagtca ggtgcacatc
     1501 tggtaatgcc tcgaagcgtc actcttttgg aggcacaacc cgcgcctgcg ccgcgcgttg
     1561 attccagagc cgggcacccc gcacggctgt tgtttcaatt ccaaggagca cctctccatg
     1621 tcgcgtatcg cgaagggcct ggtccttacc tccgttgccg ccgccgccat ggcgggcacc
     1681 gccggcatcg cctctgccga cagtggcgcg cagggcgccg ccgcccattc ccccggcgtc
     1741 ctgtcgggca acgtggtgca ggtccccgtc catgtgccgg tcaacctgtg cggcaacacc
     1801 atcgacatca tcgcgctgct gaacccggcg ttcggcaacg agtgcgagaa cgactgacgc
     1861 agcggctcag cccttgatcg gccgccctcc ccgtccggga gggcggccga tccgcgtttg
     1921 ccccgatccg tgtttgcccc gaatggcgtt tgccccgaat ggagggaggg gtggcaagag
     1981 cccctcgcgg gcccgtgcac ggcgcctcac cagggacaac ggccatcgac aacggtcatg
     2041 gcggcgagcg cgggccacta cggtgcgcgg tcgatcggtt gcacgcggcg gtcaccgctt
     2101 tcacggtgag agcaagttcc gacacaccgc gaaaggacct tccatgcatg ctctgcccgt
     2161 acggcgtatc gcgacctccg cgctctgcgc cacgtttctg ctggggatgg ccgggccggt
     2221 cgccgtggcc gccgaccacg actcggcgca cgccactcgt gccacgacgg ctcaggcgcc
     2281 ggtgcccgac gcggaagcgc tgctggcgca gaccaagggc ctcggcgaca tcgccgacgt
     2341 agtgaagccg gtcaccgacc tgctcgaaga cgtgctcaag gccgacaacg ggcagctccc
     2401 cgccgccgac gccaccaagc tcgccgacgc ggtgaaggag gccgtcgcca aggccaccgc
     2461 ggcggccccg gccagtccgc agaccccgcc gtcgacgacg ccgaccgagc cgaaggcgcc
     2521 caaccagccc gacacgccca accaacccga cacgcccaac actccgaaca cgcccaacac
     2581 tccgaacacg ccggtcgatc cgccggcggt cctgccggcg ctgcccgaca cctccgcggt
     2641 gccgcagacc gacgggcggg tggccgcgcc cgacgtcagg gccgacgcgc tcaaggccct
     2701 gcagaactcg gtcgattccc tggtcaaggc ggccaccggt ggcgacacca ccgctgtgaa
     2761 agcggcctcg acgacggtcg tgtcgggcct cgtcaacgtc gtcgccgcga tctcggtggg
     2821 cggcggcctg cccgcagcga acctggaggg tctgtccaag cagcccacgg acgcggccca
     2881 gccgtcggcg agcggcctgc tgccctcctg agccggcgtc cgaccgccgc cccaggtacg
     2941 gggacggcgg ctgaggcgaa gcaaccgccg tcccggccgt cgggccggcc cgtcacccgg
     3001 gccggcccga cggccgtcca cgggcttctc atacgagagg ccaagaggcc aagaggccaa
     3061 gaggccaaga ggctttgttc ggtggtgtgc cgcactcttc aatcgggtgg gttccaggaa
     3121 tttacggcgt acggggtttc cctcccgacc ggaggtcgtt gggcaggaaa ccgaacaccg
     3181 agaattccct ccggtgacgt gagtcaccga agaaaggaac acgatgaagt ccctgaaggc
     3241 cgccgctgtc gttgccgggt ccatggtcgt cgctggtgtc gccgcgcccg cctttgccac
     3301 cgacgtcatg cccccctcca gcctcaacgg cggcctggaa tcggccgtca cccagctcac
     3361 caccgagccg gtggacgtca tgcccctgca gtcgcagtcc agggcgctcg acacggagaa
     3421 cgaggactcc gcgctcagca ccgtcacggc cgcgacggag accctgaact cggcgagcgg
     3481 gcccacgggc ctcctgggcg gactccccct ccagaagtga cgtcggcagt cggcagccgc
     3541 cgagaggacg acgcggggcc ggtcgcgcac tgcgcggacc ggcccttcgt gtctgttcga
     3601 cccccgtccc tcgttcgtgt ggagagctcc acggaagtcc cccgagcgag tgagaaaaga
     3661 agcggcggca ttccgacacg ccactacggt tccgcgatga cctcaacctc gatttcaacc
     3721 tcaccctcaa gcggaacgca cccttcctcc gccgcgaacc tcggcacgct cgtcgtcatg
     3781 gcatggagcg gtgcacaccc cgacggtgac atgccctacc tcctggccta ctccctcggc
     3841 gacggccagg gcggacccga gggctccgcg gtcgccgtgg agcagttgct gagcgccaac
     3901 ggactgcccc tcggcgacac ggtcgtcgac gccacccggc agccgagctt cccggtcagc
     3961 ctcctcgtcg aggcaggtca ggccgtcgtc agcatgaccg agctcaaagc ccggtgcccg
     4021 gtgccgccgg agtggctcgc ggccgtcggc gaacgcggtt acgcctacct cctgttcacc
     4081 acccgcccct ggcccgaggc cgaaccgggc aagcccgtcg agccggcggc cctggcggcc
     4141 ttcgccggtg acgaggagac cctgaagaac gcggcgcacg ccctcgtccc ggcaggcagc
     4201 ccgtgcggct gaccgtatgg ccgtatggcc ggtcccgcac ttggcgggga catcagaatg
     4261 ctcgcgttgc cgcgatggtg ggatttctga acgaactgaa gatctggacg aactgaagat
     4321 tgcgccgacc ggaggaatta tgacctgatc ctgtacgccg ggtcgttacc cgttccggac
     4381 gcaccattcc gaattccgaa aggcccgtac ctgagatgaa gtcgaccacg cgaggtactt
     4441 ttgccgccgt cgtcacctgt gtggcggccg ccgtcgcgac gcccgccgtc gcggccgacg
     4501 ggattccggt cgccgtgccc ctggagggtg cggagcacgc cctggacatg gagctgccca
     4561 gcgtcagcgg caccgtgcca ctgctcaagc cgggcagccc cgacgggccg cggtacgtcg
     4621 agggccggct gctgccggac cgggccctcc cccaactgcc tgtcaccagc gatctgccct
     4681 cagcggatct gcgtacgccg gtgccgcgcg tgctcggcga caccttcgac cacctcgccg
     4741 tcggcgcacc cacctcctcc ggcctgcgcg cccggacgcc cggtgcctcg ctggacggcc
     4801 cgctgaccgc gccgcgcccg gaccggctcg gcctcccggc cccgaaactg cccgaggccg
     4861 gcctgctcgc cccggtcctg caagcggcgc cgggggccac cctgggcctc gcgcccggac
     4921 tgtaggcgca ccgaacagtg cgggtgggag cggggaaccg tacgcggcgg gacgtcctga
     4981 gaggggtgct cgccgcggcc atggccgtcg cgctgactcc gctggtcacg gcgtcgaggc
     5041 gagcacgcgc cgagccgtac accgccggca cgccgatcgg gttcgaggcg gagtccgagg
     5101 ctgagtccga gtctgagtct gagtccgagg cgcggttcgc cgccgggttc gacgagatgt
     5161 acggcggctc ccggatccgc gggttcaagg acacggcggg cgtgtgctcg gacggggtcg
     5221 cgcgtgcggc caccgccggg ccgtggcggg tcacggtgga cgaccggcca ctgcatctca
     5281 tgcggcgggc cgacggcggc tatctgacga tgctcgacca ctaccagtcg tacccgaccc
     5341 cgctggccgc cgcccgcgct gccgtggacg aactcgacgg cacactgcgg ctgcgcgagg
     5401 ccaccacggg ggcggaacag ggcgaaggac acggaggacc cggaggatac ggagatggcg
     5461 tacccgcgta agaacgtgag cgcgctgacg agcacggaga agcgcaggct cgtcgacgcg
     5521 ttcctggaga tcaaacgcac cggcgagtac gacgagttcg tccgtatgca catcgaccac
     5581 tacgtgtcgg acggcgaggg cggactgcgc gcggcccata tggcgccgtc cttcctgccc
     5641 tggcaccgga agttcctgct ggacctggag cgggcgctgc gccggacgga ctccggggtg
     5701 tccgtgccgt actgggactg gacgaaggac cgcacccccg ccgccccgcc gtggggggag
     5761 gacctcctcg gaggcaacgg gcgtcggtcc gaccaccagg tgacgaccgg cccgttcgcc
     5821 cgccggcaca agaagtgggt cgtcaaggag tcgatgacgg acggcgacta cctcacacgc
     5881 gatttcggca ggccccgcga tccgctcgcc ctgcccaccg cggacgatct cgcgaaggcc
     5941 ctcgccgatc ccgtgtacga cacggagccc tgggactcca cctccggccg cggcttccgg
     6001 aacggtcttg aggggtgggg gaccgggcgg ggcaacgacg cctggcgcaa ccacaaccgc
     6061 gtccaccgct gggtcggcgg gcacatgctg ggcgggggct ccgtcaacga ccccgtcttc
     6121 tggctgcacc actccttcgt tgacctgctc tggacgcgct ggcagaagcg gcatcagggc
     6181 gcgcggtatc tgcccaagtc gccgccgggg ctcggggacc ggcagcgcgg acggatcgtg
     6241 gcacggcacg aggcgatgcc gccgtggggc gtgacaccgg acgagatgga ggaccacagc
     6301 cggatctacc ggtacgtgtg aggcggcgac gcggaacacg gcgtacggcg aacactgcgc
     6361 aaggcaggcg aacactgcgc aaggcagaag gaagcccccg gcgggcggtg ccgtcggggg
     6421 cttcgcgtcg gtggggtggc tcagtggccg tagccgtggg gggtgtcgtc gatgttcgcg
     6481 cactcgttgc cgaacgtcgg gttcagcagg gcgatgatgt ccacggtgtt gccgcagagg
     6541 ttgaccggga tgtggagcgg aacctggatg acgttgcccg acgcgacgcc gggcgagccg
     6601 acggccttgc ccgcggcgtc cgcgtccgcc atggccagag aggcaccact gagcacaacg
     6661 gcgcccgtgc cgagcgcgac accggctgcc ttcgcgatgc gagacatcac gtttctcctt
     6721 cgaattcggt gagcacggca gcacgaacgc gaccgcactg tcccttcaac gcgaggattg
     6781 acagcgggta acggcaccca gcatgacaac atccagcaaa atgccccaaa cgggtcgcct
     6841 gcgggtgagg gtcgcgcctc ccgagtgtcc gcgtgctcac cagggcacgg aggttccgtc
     6901 ccaggagaag aacgcgccgg tggggccctc gtccggcagg agggccagtc ggacggcttc
     6961 ctgggcggcc tcggccgggt ctccgccgga ggcggccgcc cgggtgttga ggtcggtggc
     7021 ccgcaggccg ggggcgagcg cgttgacctt gaagccctcc tcggccagcg tctgggcgta
     7081 gaagacggtc agggcgttga gggcggtctt cgacgatcga taggcggcgg cggcaccgtt
     7141 tccgggggcg aactgcgggt tggggttcgt gctccaggtc agcgatccgg tgccacttga
     7201 aacgttgacg atgcgggggc ggggcgagcg gcgcagggcg ggcaggaagg cgttggtgac
     7261 ctcgacgacc gcgaacacat tggtctcgta cgtacgccgg aactcctcga cgccggcctc
     7321 ggtcggcggg gtgagcgaca cggagatgcc cgcgttgttg accaggacgt ccaggcggtc
     7381 cacctgcgcc gcggcctcgg cgacgctccg ggggtccgtc acgtcgagga ccagcgggcg
     7441 ggcgtcgccg acgatctcct cggcggcccg ctgcccgcgt ccgacgtcgc gggaacccac
     7501 gtacacggtg aagccctccg cggcgagttg ggcggcgatg tgcttgccaa tacctttgtt
     7561 ggcaccggtg accagtgctg tgcgatcgtt catgacgccc atggttctgc ggctgtggcc
     7621 gtgctgccag ggacaacctg taccaggcgg tggtgggagg aggcacagga tggcgcggga
     7681 ggaactggcc cgttttctgc gggaccgtcg tgaggggctg tgtccgcacg agctcggtct
     7741 gccgacgagt actcctcgcc gtacaccggg tctgcgacgc gaggaggtgg cggagctggc
     7801 ccacatgtcg gtcgactact acacacggct ggagcaggcc cgcgggcccc ggccgtcgcc
     7861 ccggatcctg gacgcgctgg cccaggccct gcggctcgtt ccggcggaac gcagccacct
     7921 gttccgcctg gccggctcct ccctgccgcc cggcacccac gccgtgcagc gggtgcgccc
     7981 gcaggtggcc gggatgctgc ggcgactgcc ggagaccggc gcgatcgtca ccgacgcggc
     8041 ctatggcgtc gtcgcctgga accccctcgc gcaggctctg ctgggcgatg acctgggacg
     8101 cgggacgacg aacctggccc ggcgccgctt cctcggccag gggcggagtt acgagagctc
     8161 cagcgcggag aggttcgggc acatcgtggt ggcgcggctg cgccgggccg cggaccgcta
     8221 cccgcacgac gcgcggctcg ccggtctgct gagcgaactg cgttccggca gcgaggagtt
     8281 ccggcagatc tgggaaaccc gcccggtcca cgctcccggg caccgcacca aggtcctcga
     8341 ccacccgacg gcaggcagac tgcggttgaa ctgcgacgta ctgctcgtcc ccgaggacga
     8401 ccaggaagtc gtcctgatga cggcggatcc cgggtcgccg tccgcgcgga ccctgcgcgg
     8461 gctcgccgca cgacttgacc gagtggccgt tgacgccgcc gagctcggtt cctgcgagca
     8521 ggaacctgtt ccgcaccgtc gccgtcggtg acccgtcaga cgagggaagc cagcagcgag
     8581 agtttctcgg cactgtcgct gcctgcgtcg gcgtagtaga gcaccaggag gagtccgttc
     8641 acgggcagct tgtcgcggtg caggtgcaac cggccgacga ccggatggtt cacggtcgtg
     8701 gtaccgccct caagtgcccg gacgtcgtgc cgggcccaca gttcgcggaa ccggccgctc
     8761 gtgagggaca gctcgccgat gagctccacg aaccgcgggt tgtcgacatc gtcgccgatc
     8821 gactctcgca acgcggcgat gaaccactcc gaggacgccg tccagtccgc gtggaacgac
     8881 tgttcctccg ggtccagcag cagcgagcgc aggcggttct cgcccggccg caggcggggt
     8941 gagaacgccg tcgccagctc gttggccgcg aggacgtcga aggcacgccc ctcgacgaac
     9001 gccggcactc ccacggccgc gagcaactgg tgcagccggg ccggtacgtg ctcggccgca
     9061 cggcgtcgac gactccgctg ccgatcccga tcccgctgtc gcggccgtgg agtggccagg
     9121 ctgtgcaggt actcggcctc gacctcgtcg agccgcagta cgcgagcgag cgcgtcgagc
     9181 acctgggccg acgggtgggt gtcgcggccg cgttcgagac gcaggtagta gtcggcactg
     9241 atgccggcga ggatcgcgag ctcctcgcga cgcaggccgg gcacccggcg gatgccgccc
     9301 gccggaatac cggcctgttc cggcgtgacg agtccgcgac gggcgcgcag gtaggcgccg
     9361 agccggttgg cgtcctggtc cgctgtcatg cgaacacgct agcttccgga ccggccggag
     9421 cttttggacc tgcccggaag catcgagagt gcctgtgagc gctgggccac gagttcggcg
     9481 taggtaccgt cgcgttctgc ccaacggtgc atgagcacgc cctgtaccag gatctcgggc
     9541 ggtgtcgggt cctgcgcgag caggtccatc acttccgcgc cgtaggcgtc gagggcgaga
     9601 gcgtgcgggt tgccgctccc gagcggggtc gcgacggccg gcgggacgag ttcggcgacc
     9661 tcgacaccgg tcccggccag ctgcgctcgc agcgcctccg tgtaggcgtg cacggcggcc
     9721 ttcgaggcgc cgtaggtggg catcagcggg aaggggagaa aaccgatccc ggagctgaca
     9781 gtgatgatcg tgctgttgct gttgccgccg ttgccgcgag cgaggaggtg cggggtgaac
     9841 gcgtcgatga cccggatggt gccgagtacg ttcacggcga ccgtcgtttc ggcggcaccg
     9901 aagtgggtcg ggtcgcggag gtcctcgggg atgccggtgc ccgccatggt gatgacggtg
     9961 tcgaggccgg gatgcgttcg cagcacctcg tcgcgcgccc ggatcaccga gccggcgtcg
    10021 gtgacgtcga tctcgacggg gacgagtccc tcgtcggcga gccgcgtgaa cgtatcggtg
    10081 cgacggccgc cgacgacgac ggtactgccg gcggcgcgga accggcgggc cagctcaaga
    10141 ccgatgcccg aggtggcacc ggcgatgaaa acggtgcggg aggtgatgtc catggctcga
    10201 cggtaggccg gatctccgtg ggctcagggg gccctgtcag gaccccggaa cgccacaccc
    10261 tcgtacgccg cacctctggc ggccatcgcg tgtgggcgta cgtacgctga tcggccgccg
    10321 gctcgtcggc acgtgcgctg accagccggc ggaccacgac ggcggaccac gacggaggag
    10381 cgcgacggct ggctgtcggc gtgctggacc aggctgacct cgaccggatc ggcgtccgcc
    10441 agtggtaccc ggaccgggcc ggtcacggac t
//
