LOCUS       NZ_AP023440            11314 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces aurantiacus strain JCM 4677 chromosome.
ACCESSION   NZ_AP023440
VERSION     NZ_AP023440.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces aurantiacus
  ORGANISM  Streptomyces aurantiacus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 9428393)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye
            Genome Coverage                   :: 62X
            Sequencing Technology :: Illumina HiSeq X Ten; PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014701115.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 05:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,211
            CDSs (total)                      :: 8,122
            Genes (coding)                    :: 7,928
            CDSs (with protein)               :: 7,928
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 69 of 194
            Pseudo Genes (incomplete)         :: 161 of 194
            Pseudo Genes (internal stop)      :: 17 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:48:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2138956
            Orig. end                         :: 2150270
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023440.1.
            Genome sequencing and analysis were conducted under the Global
            Catalogue of Microorganisms (GCM) 10K type strain sequencing
            project (http://gctype.wdcm.org). The genomic data are also
            available at http://gctype.wdcm.org/sequencing/GCM10017557
            Annotated by DFAST v.1.2.7 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11314
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="15"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11314
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11314
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6314](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6314
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(775..1230)
                     /locus_tag="IGS71_RS08745"
                     /old_locus_tag="GCM10017557_18020"
     CDS             complement(775..1230)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987569.1"
                     /locus_tag="IGS71_RS08745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_18020"
                     /product="hypothetical protein"
                     /protein_id="WP_190849893.1"
                     /transl_table=11
                     /translation="MPDPNHAVYEACFTRSLLQLLSGAGRRRLVAFRVDAEGVLLGGAP
                     ARYKAQTAFVPWEDITSVVVWQQRMAGPSMDYIGVQRRTGAPALPGMNAGLRPDQTAEL
                     APHVEHDLFLASRPVNRWRLDPEGLQAAVEAFAPGVSVLVHEQRNAK"
     misc_feature    1305..1319
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.83"
                     /tool="antismash"
     gene            1339..2160
                     /locus_tag="IGS71_RS08750"
                     /old_locus_tag="GCM10017557_18030"
     CDS             1339..2160
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657146.1"
                     /locus_tag="IGS71_RS08750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_18030"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_190849894.1"
                     /transl_table=11
                     /translation="MNTFVRSVIAACVVGAGVLTACGAPEAPRAATVVESATGVASRPP
                     TLAPGPGGLTPVFERASRARGKTVALTFDADMTADQGPRAAAGERFDNPRLIRTLRRLN
                     VPATVFMTGRWAEEYPRQARAIGRDPLFEVANHSYSHHAFTEDCYGLPTVPVTGMRADV
                     ERAFTAFRKAGIAHPMPYFRFPGGCYDHRALTSLSTTGVTAVQWDVVSGDAFATDAGAV
                     AREVLDGVRPGSVVVMHCTRSAAPATEEALRTIVPELRERGYRFVKVSELI"
     gene            2338..3198
                     /locus_tag="IGS71_RS08755"
                     /old_locus_tag="GCM10017557_18040"
     CDS             2338..3198
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134699.1"
                     /locus_tag="IGS71_RS08755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_18040"
                     /product="aldo/keto reductase"
                     /protein_id="WP_055518384.1"
                     /transl_table=11
                     /translation="MQTRAIGDVRVGAIGLGGMPMSIEGRPDEARSISAVHAALDAGVT
                     LIDTADAYHRDANEVGHNESLIARAVAEYGGDTSDVLIATKGGHLRPGDGSWTLNGSPE
                     YLKKACDASLKRLGVEAIGLYQFHRPDPKVPYGESVGAIRDLLDAGKIRFAGISNADPD
                     RIRQANEILGGRLVSVQNQFSPAFRSSEPELELCAELGIAFLPWSPLGGISKAGELGSR
                     FAVFADVAGEHGVSPQQVSLAWTLAKAPVVIPIPGSTRPETIRDSVAAAELKLSAEELT
                     RLDAA"
     gene            3282..4067
                     /locus_tag="IGS71_RS08760"
                     /old_locus_tag="GCM10017557_18050"
     CDS             3282..4067
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981772.1"
                     /locus_tag="IGS71_RS08760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_18050"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_190849895.1"
                     /transl_table=11
                     /translation="MSSDETTTAAGAGAQDSPFRHERTARDEAPQFVLPLVVRIERNEP
                     PARTDALETAARAVLVLLSDERSLGEGEGEGETEGEGEWARAVRDWQDSRIRKVVRRAR
                     GAEWRRAEALPGITVTGKSAEVRVFPPIPLDGWPKDLARLQVSGTDLDEPEPPADADTA
                     APVLWLSPSLDMSAGKAMAQAGHGAQLAWWELSDEDRTAWREAGFPLAVRTARPAQWER
                     LITSKLPLVRDAGFTEIAPGLTVAVEGVDRVHALPRREH"
     misc_feature    4275..4288
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.13"
                     /tool="antismash"
     gene            4275..4877
                     /locus_tag="IGS71_RS08765"
                     /old_locus_tag="GCM10017557_18060"
     CDS             4275..4877
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481898.1"
                     /locus_tag="IGS71_RS08765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_18060"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_190849896.1"
                     /transl_table=11
                     /translation="MRRINGTALVVVALLGTLDALPFPVWSYTGRAGPGPADLAAGTVP
                     TRWGLLTASDRDLLVRLRLAGLWQLPAARQALERSPGGALKEAADQLITGHTGLDRRVR
                     VVARDLGVELPGRPTAQQRGRLRQLTAASDEDYEKVWADLLRSAHGTLFPAIGAVRNET
                     RNSLVRQLASAAHQVVLDHITVLETCVGRSSEPQMLL"
     misc_feature    complement(4974..4989)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.15"
                     /tool="antismash"
     gene            5001..6314
                     /locus_tag="IGS71_RS08770"
                     /old_locus_tag="GCM10017557_18070"
     CDS             5001..6314
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122140.1"
                     /locus_tag="IGS71_RS08770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_18070"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_190849897.1"
                     /sec_met_domain="DUF692 (E-value: 8.2e-96, bitscore: 319.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKHLGTGIGWRPEIADAVERMPGIDWVEAVAENVCPGHLPESLVR
                     LRERGVTVVPHGVSLGLGGAERPDEARLADLAARAEALGSPLVTEHIAFVRAGGALTAS
                     PLLEAGHLLPVPRTRDALDVLCANIRIAQDALPVPLAVENIAALISWPGEEMTEGQFLY
                     DLVDRTGVRLLIDVANLHTNHVNRGEDPARALDELPVEAIAYVHVAGGFERDGVWHDSH
                     AHPVPDVVLGVLAELASRVRPPGVLLERDENFPEPAELERELTAIRETVEKGAMTAGAA
                     ATAKTGGTPPETGDAPETGPARQRLALTQAALLSALVAGTPVPEGFDRTRLAVQARALA
                     GKRADVVGKVAPELPEILAGSYRKTFLAYAHSHPMTDGYRRDALDFAEYVLFGGHLDDP
                     DVRRGLSDWWLERSGPAPLSRRPAARLARATRRVLLRR"
     gene            6496..7323
                     /locus_tag="IGS71_RS08775"
                     /old_locus_tag="GCM10017557_18080"
     CDS             6496..7323
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983659.1"
                     /locus_tag="IGS71_RS08775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_18080"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_190849898.1"
                     /transl_table=11
                     /translation="MRSINGRGLFSGTGLIITGLAVTLVALVFPIWSYADRSGTGVDTL
                     NATTVSTRFGPLSALDREFVTKVRLAGLWELPAGRQAAERGTTKAVRTAGEHLVEGHAF
                     LDERVRDVAAQLGLELPNQPSEQQRAWLATLSSAHGRQYDTQFANILRGAHGKVFGLVA
                     QVRANTRNSLVRDLADDANTTVLDHITVLEATGLVDFDALARDAASASAPPLTVSPAPP
                     GPEDSPSPPVPATPSPAPSYSLPPAATGPPQEDDRREGGPKGADGPKGASSRS"
     gene            7446..9032
                     /locus_tag="IGS71_RS08780"
                     /old_locus_tag="GCM10017557_18090"
     CDS             7446..9032
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (113-480). E-value:
                     2.9e-21. Score: 68.1. Matches aSDomain:
                     nrpspksdomains_IGS71_RS08780_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432060.1"
                     /locus_tag="IGS71_RS08780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_18090"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_190849899.1"
                     /sec_met_domain="PF00561 (E-value: 1.3e-24, bitscore: 86.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVLLYGTAGSLVLTAVVAAPAGSAQTSSGRAERHGTAVAAERA
                     AKAGIRFGTCSEDQDGPGTLQCGTVTVPLDYAHPDGKKIKLTVSRVRATGKDSKGGKDG
                     KGSVPRQGSLVYNPGGPGASGMYFPLIGMVPEWKRIASAYDLVGYAPRGVGRSAPLSCQ
                     DPKTLRKSPTQSPTHPSESYKKERIARAKAYAQGCVQRSGRSIRHFSSVNNARDLDVLR
                     AALGERKLTFMGASYGTYFGTLYAELFPSHVRRMVLDSAVNPDPAKVWYRNNLDQSLAF
                     EGRWADFRAWIAKHHKVYGLGRTPQEVQRSYERARARLAAKPAGGKVGPGQLRGAFLQA
                     GYYDDYWPQRAAALSAYLKGDPKPLIRQAGPHRDGVKEQENSKAVYTAVECNDAPWPRS
                     WHTWDRDNTRLAHAAPFETWDNVWTNLPCAYWPLPRTRPLDVRTSPGELPPTLILAAER
                     DAATPYAGALETHRRLSGSTLVTERASGSHGIGGGANACVNAHLDAYLLRGRLPAPEAT
                     CAPHKEPTPAP"
     aSDomain        7785..8885
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IGS71_RS08780_Abhydrolase_1.1"
                     /evalue="2.90E-21"
                     /label="IGS71_RS08780_Abhydrolase_1.1"
                     /locus_tag="IGS71_RS08780"
                     /protein_end="480"
                     /protein_start="113"
                     /score="68.1"
                     /tool="antismash"
                     /translation="LVYNPGGPGASGMYFPLIGMVPEWKRIASAYDLVGYAPRGVGRSA
                     PLSCQDPKTLRKSPTQSPTHPSESYKKERIARAKAYAQGCVQRSGRSIRHFSSVNNARD
                     LDVLRAALGERKLTFMGASYGTYFGTLYAELFPSHVRRMVLDSAVNPDPAKVWYRNNLD
                     QSLAFEGRWADFRAWIAKHHKVYGLGRTPQEVQRSYERARARLAAKPAGGKVGPGQLRG
                     AFLQAGYYDDYWPQRAAALSAYLKGDPKPLIRQAGPHRDGVKEQENSKAVYTAVECNDA
                     PWPRSWHTWDRDNTRLAHAAPFETWDNVWTNLPCAYWPLPRTRPLDVRTSPGELPPTLI
                     LAAERDAATPYAGALETHRRLSGSTLV"
     gene            complement(9137..9883)
                     /gene="hemQ"
                     /locus_tag="IGS71_RS08785"
                     /old_locus_tag="GCM10017557_18100"
     CDS             complement(9137..9883)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675654.1"
                     /locus_tag="IGS71_RS08785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_18100"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_055518839.1"
                     /transl_table=11
                     /translation="MSDDAPITGQADRTKTPNAGKKAKDLNEVIRYTLWSVFKLRDVLP
                     DGAARAGYADEVQELFDQLTAKDVTIRGTYDVSGLRADADLMIWWHAETSDQLQEAYNL
                     FRRTRLGRALEPVWSNMALHRPAEFNRSHVPAFLADETPRNYISVYPFVRSYDWYLLPD
                     EDRRRMLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELDRIVDLMRHLRASEA
                     RMHVREEVPFYTGRRKSVTELVEGLA"
     misc_feature    complement(10891..10906)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.58"
                     /tool="antismash"
ORIGIN
        1 gccgtcgtcc ggggtggtga cttccttcca gccggtgccg tcgcgccact gggtgcgcag
       61 tgacgtgatc ccctcctggc tcggcgattc ggggcaggca tcctggtcag cgggactgtc
      121 cgagtccgcc gcgcaggcgc tgtactccgc ctggacctga gccttcgggt acgcgctgtg
      181 cgagtgattg gtgaccgtgt acaccacctc ggtccagcgg ccttcgcgca cggtcgcggg
      241 gcccttgggg gagacggtgc cgagcgacag ggtggccagc ggcgcggtcc ggtgctcccg
      301 tgcgagggtg cgtgagccgt ccgtcatcgt gacggccacc ggaaggccgt cgccttcgac
      361 cctggggccg acgtgcggag cgatacgcag gaacacgaga gagctgcccc gcggcaggtc
      421 catacggaag gtgccggtcg ccccctgttc gtccggggtc agttcgacct tcctccagcc
      481 gccgtcgagc ttgcgttcca ggcccggccg gggatcgccg gggtggccga gtccgatgcg
      541 gacccgtacg gcgacatcgg cccggaaacg ctcctcgttg gagaggtcga gcgccaccac
      601 gggccgggtg tccccctgag cgtcgacggt tttcatggag agccacaccg gcgatgacgc
      661 cgacgcactc ggctttggac cagtcgcgtt ccctccgccg cacgccgcca cagcggtgag
      721 tacgacaagg acgcccacga gccgtacgta cctcaactgt ccctcagatg cctgctactt
      781 ggcatttcgt tgctcgtgga cgaggaccga caccccggga gcgaacgctt cgacggcggc
      841 ctgcagaccc tcggggtcga ggcgccaacg attgacgggg cggctggcga ggaagaggtc
      901 gtgctcgacg tgaggagcga gttcggccgt ctgatccggc cggagtccag cgttcatgcc
      961 cggcagggcc ggtgctccgg tccgtcgctg gacaccgatg tagtccatcg acggaccggc
     1021 catccgctgc tgccacacca cgaccgaggt gatgtcctcc cacggcacga aggccgtttg
     1081 cgccttgtat cgggccggag caccaccgag cagcacgccc tccgcgtcga cgcggaacgc
     1141 gaccagacgc ctccgccctg cccccgacag cagttggagg agggagcggg tgaagcaggc
     1201 ctcgtacacg gcgtgattcg gatcaggcat ggccagaggt tatcgccggc gacagcgccc
     1261 acccgggacg gggaggtggt tgcgacaccg gaagcgatat aagctgtttt tcgttttcgt
     1321 ccggttaatg tcggctgcgt gaacaccttc gtacggagtg tcatcgcggc ctgtgtggtg
     1381 ggtgccggcg ttctcacggc gtgtggcgct cccgaggccc cccgtgccgc cacagtggtc
     1441 gagtccgcca ccggcgtggc ctcccggccg cccaccctcg cgcccggtcc cggtgggctg
     1501 acgcccgtct tcgagcgtgc gagccgggcg cgcggcaaga ccgtcgccct gaccttcgac
     1561 gccgacatga cggcggatca gggaccgcgg gccgccgccg gggagcgttt cgacaacccc
     1621 cggctgatcc ggacgctgcg gcggctgaac gtgccggcga ccgtgttcat gacggggcgc
     1681 tgggccgagg agtacccccg gcaggcgcgg gccatcgggc gtgacccgct cttcgaggtc
     1741 gcgaaccact cctacagtca ccacgccttc accgaggact gttacgggct gccgaccgtc
     1801 cccgtgacag ggatgcgggc ggacgtggag cgggcgttca ccgcgttccg caaggccggg
     1861 atcgcgcatc cgatgccgta cttccggttc cccggcgggt gttacgacca ccgggcgctc
     1921 acgtcactga gcaccaccgg tgtgacggcc gtgcagtggg acgtggtgag cggggacgcg
     1981 ttcgcgaccg acgcgggggc cgtggcacgg gaggtgctgg acggcgtgcg gcccggttcc
     2041 gtggtcgtca tgcactgcac gcgcagcgcg gcacccgcga ccgaggaggc cctgcgcacg
     2101 atcgttcccg agcttcgcga gcgcgggtac cggttcgtga aggtgtccga actcatctga
     2161 tccgctccca gccgatccga ttcatcacct gcgctgctca cagcgatcgg attctgttgt
     2221 tggacggcta aaacgtcaca gtggaggctg gtaaccagcc aagcaccgca ctccccaagt
     2281 ccccaagtcc cgaagtattg aagtttctaa gttccgaact cccaggagga gacccccatg
     2341 cagacacgcg ccatcggtga cgtccgggtc ggagcgatcg gtctgggcgg gatgccgatg
     2401 tccatcgagg ggcgcccgga cgaagcgcgc tcgatctcgg ccgtgcacgc ggcgctggac
     2461 gcgggcgtca cgctcatcga caccgcggac gcctatcacc gggacgccaa cgaggtcggt
     2521 cacaacgagt ccctgatcgc ccgcgcggtg gccgagtacg gcggcgacac ctcggacgtg
     2581 ctgatcgcga cgaagggcgg tcatcttcgt ccgggcgacg gctcctggac cctgaacggc
     2641 tctccggagt acctgaagaa ggcctgcgac gcctcgctca agcggctcgg tgtcgaggcc
     2701 atcgggctct accagttcca ccggccggac ccgaaggtcc cctacgggga gtcggtcggc
     2761 gcgatccgcg atctgctgga cgccggcaag atccgcttcg cgggcatctc caacgccgac
     2821 cccgaccgga tccggcaggc caacgagatc ctcggcggcc ggctggtgag cgtgcagaac
     2881 cagttctcgc ccgccttccg ctccagcgaa ccggagctgg agctgtgcgc ggaactcggc
     2941 atcgcgttcc tgccctggag cccgctgggc ggtatctcga aggcgggcga gctgggctcg
     3001 cgtttcgccg tgttcgcgga cgtggcgggc gaacacgggg tgagcccgca gcaggtgtcc
     3061 ctggcgtgga ccctcgcgaa ggccccggtg gtgatcccga tcccgggctc cacacgcccc
     3121 gagaccatcc gcgactcggt cgccgcggcg gagctgaagc tgtccgccga ggagctgacc
     3181 cgcctcgacg cggcgtgacg gtgaacggtg ggcgatgaac ggcaggcgac gggcggtgga
     3241 cgcgcgatcg atccaccgcc cggtcctggc agactgggcg cgtgagcagc gacgagacaa
     3301 cgaccgcggc gggcgccgga gcccaggaca gccccttccg gcatgagcgg accgcccgcg
     3361 acgaggcacc gcagttcgtg ctgccgctgg tcgtccgtat cgaacggaac gaaccgccgg
     3421 cgcgtacgga cgcgctggag accgcggccc gtgccgtcct ggtcctcctc agcgacgaac
     3481 gctcgctcgg cgagggcgag ggcgagggcg agaccgaggg cgagggcgag tgggcgcggg
     3541 ccgtccgcga ctggcaggac tcccggatcc gcaaggtcgt ccggcgggcg cgcggcgccg
     3601 agtggcgtcg ggccgaggca ctgcccggca tcaccgtcac cggcaagtcg gccgaggtcc
     3661 gcgtcttccc gccgatcccg ttggacgggt ggcccaagga cctggcccgc ctccaggtct
     3721 ccggcaccga cctcgacgaa ccggagccgc cggccgacgc cgacacggcc gcacccgtgc
     3781 tgtggctgag cccgagcctg gacatgtcgg ccggcaaggc gatggcccag gcgggtcacg
     3841 gcgcccagct ggcctggtgg gagctgtcgg acgaggaccg tacggcctgg cgtgaggcgg
     3901 gcttcccgct cgccgtccgc acggcccgcc cggcgcagtg ggagcggctc atcacgagca
     3961 agctgccgtt ggtgcgtgac gcgggcttca cggagatcgc acccgggctg actgtcgcgg
     4021 tcgaaggagt cgaccgcgtc cacgctctgc cgcgccggga gcactgagac ctcattccgg
     4081 tcctgggcga ggccccccgc gtcgaacggc cccggcgttg aacggccctg ccgtcccgta
     4141 cgtaccttcc ggcgtcggcc gagtgtcggt ccaagcgcgc gtatcagtcc aagcgctggt
     4201 ccaggtgccg gcccgaagta ctggcccaag tgctggttcc caacgaccgg ttggcgtgtc
     4261 ccgggaggca ccttttgcgg cgtatcaacg gtacggccct cgtcgtcgtg gcgctcctcg
     4321 gcacgctcga cgcgctcccg ttccccgtgt ggtcgtacac cggccgcgcc ggcccgggtc
     4381 ctgccgacct cgccgccggg accgtaccca cgcgttgggg tctgctgacc gcctccgacc
     4441 gcgacctgct cgtccggttg cggctggcgg ggctgtggca actgcccgcc gctcggcagg
     4501 cacttgagcg ttcccccggc ggcgcgctga aggaggccgc cgaccagctg atcaccggcc
     4561 acaccggcct cgacaggcgg gtacgggtgg tcgcgcggga cctcggcgtc gaactgccgg
     4621 gccggccgac cgcacagcag cggggccggc tgcggcagct gaccgccgcg agcgacgagg
     4681 actacgagaa ggtgtgggcg gacctgctgc gctccgcgca cggcacgctc ttcccggcga
     4741 tcggggcggt acggaacgag acgcgcaact cgctggtacg gcagctcgcc tcggctgccc
     4801 accaggtcgt gctcgaccac atcacggtgc ttgagacgtg cgtgggtcgc tcgtccgaac
     4861 ctcaaatgtt gctctgaacg tccccgtcga cgggttcgtc cccggtttcc ggggccatca
     4921 ctccggcacg ccggaacgac aggttcgtcg aaggagacgc cgcacaggtc ctggtgaaac
     4981 cgtggtgaag gagggggacc atgaagcact tgggtacggg aatcggctgg cggccggaga
     5041 tcgccgacgc cgtggaacgc atgcccggca tcgactgggt cgaggccgtc gccgagaacg
     5101 tctgtccggg acatctcccc gaatcgctgg tacggctgcg cgagcgcggc gtcaccgtgg
     5161 ttccgcacgg ggtgtcgctc gggctcggcg gcgcggaacg ccccgacgag gcgaggctcg
     5221 ccgacctggc ggcccgtgcc gaggccctcg gctccccgct ggtcaccgag cacatcgcgt
     5281 tcgtccgggc gggcggggcg ctcaccgcct cgcctctgct cgaagccgga catctgctgc
     5341 ccgtcccccg cacccgggac gccctcgacg tcctgtgcgc gaacatccgc atcgcccagg
     5401 acgcgctgcc cgtgccgctc gcggtggaga acatcgcggc gctgatctcg tggcccggcg
     5461 aggagatgac cgaggggcag ttcctgtacg acctggtcga ccgcaccggc gtacgcctgc
     5521 tcatcgacgt ggccaacctc cacaccaacc acgtcaaccg cggcgaggac cccgcgcggg
     5581 ccctcgacga actgcccgtc gaggccatcg cgtacgtcca tgtcgccggt ggtttcgagc
     5641 gcgacggcgt ctggcacgac agccacgcgc accccgtgcc cgatgtcgtc ctcggcgttc
     5701 tcgccgaact ggcctcccgg gtccgcccgc cgggcgtgct cctggagcgc gacgaaaact
     5761 tccccgagcc ggccgaactg gagcgcgagc tcacggcgat acgggagacg gtggagaagg
     5821 gggccatgac cgcaggggcc gccgcgaccg cgaagaccgg tggtacgccg ccggagaccg
     5881 gtgacgcgcc ggagaccggc ccggcccgcc agcgcctcgc cctgacgcag gccgccctgc
     5941 tgtccgcact cgtcgccggg actcccgtgc ccgaggggtt cgaccggacg aggctggccg
     6001 tgcaggcgcg ggctctggcg gggaagcggg ccgacgtcgt gggcaaggtc gctccggagc
     6061 tgccggagat tctggcgggc tcctaccgga agaccttcct ggcgtacgcg cacagccacc
     6121 cgatgaccga cggctaccgg cgggacgcgc tggacttcgc cgagtacgtg ctgttcggcg
     6181 ggcacctcga cgacccggac gtacggcggg gtctgagcga ctggtggctg gagcgctcgg
     6241 ggcccgcgcc actgtcccgg cgcccggcgg cccgcctggc ccgcgccacg cgacgggtgc
     6301 tgctgaggcg ctgagggcct gagtcgctga gaggctgagg tgctgagaac gggtcgatga
     6361 ctggcgtgga ccgggagcgt agaggatccc tcgtacccct cgccgcaccc gtatcgcggt
     6421 tggcgtactc ggcgaagtaa tatgccaacc cataccagaa gcgcactaac gtgcggtgcc
     6481 gcatcaggag gcaccatgcg atccatcaac ggccggggac tgttcagcgg caccgggctc
     6541 atcatcacgg gtctggcggt gaccctcgtg gcgctggtct tcccgatctg gtcgtacgcc
     6601 gaccggtcgg gcacgggcgt ggacacgctg aacgccacga ccgtgtcgac gaggttcggc
     6661 ccgctgtccg cgctggaccg cgagttcgtc acgaaggtcc ggctcgccgg cctgtgggag
     6721 ctgccggccg gccggcaggc cgcggagcgc ggcaccacca aggccgtacg gacagcgggc
     6781 gagcacctcg tcgagggcca cgcgttcctg gacgagcgcg tacgcgacgt ggccgcccaa
     6841 ctcggcctcg aactgcccaa ccagccgagc gaacagcagc gggcctggct cgcgacgctg
     6901 agctcggcgc acggcaggca gtacgacacg cagttcgcga acatcctccg cggcgcgcac
     6961 ggcaaggtgt tcggcctggt cgcccaggtc cgtgcgaaca cccgcaactc actcgtccgc
     7021 gacctcgccg acgacgcgaa caccacggtg ctcgaccaca tcacggtcct ggaggccacc
     7081 ggcctcgtcg acttcgacgc cctggcccgt gacgcggcct ccgcgagcgc gcccccgctc
     7141 accgtctccc ccgcgccgcc cggcccggag gactcgccca gcccgcccgt cccggccacg
     7201 ccctcccccg caccgtccta ctccctgcca ccggccgcga ccggcccacc gcaagaagac
     7261 gaccggcggg agggcggacc gaaaggggct gacgggccga aaggggcttc gtcgcgctcc
     7321 tgaacgcgtg ccgggcgcgc atcggggtca ttgggccgac acctgcgagg cggtccttta
     7381 cttcgtcaag tcccgcacga aacatccctt ttgttgctgc cgtgcatggc actgaaggga
     7441 aagcgatgag agctgtcctc ctctacggaa cggccgggtc cctggtcctg accgctgtcg
     7501 tcgccgcccc cgcgggcagc gcgcagacct cgtccggccg ggccgagcgg cacggcacgg
     7561 cggtcgccgc cgagcgcgcc gccaaggccg gcatccgctt cgggacgtgt tccgaggacc
     7621 aggacggccc cggcactctg cagtgcggca cggtgacggt cccgctcgac tacgcgcacc
     7681 ccgacggcaa gaagatcaag ctgaccgtca gccgggtgcg ggccaccggc aaggacagca
     7741 agggcggaaa ggacggcaag gggtccgtgc cccggcaggg ctcgctcgtc tacaacccgg
     7801 gcggccccgg cgcgtccggc atgtacttcc cgctgatcgg catggtcccg gagtggaagc
     7861 gcatcgcgtc ggcgtacgac ctcgtcggtt acgccccgcg cggggtcggc cgctccgcgc
     7921 cgctgtcctg ccaggacccg aaaacgcttc ggaagagtcc cacacagtcg ccgacgcacc
     7981 cctcggagtc gtacaagaag gagcgcatcg cacgggcgaa ggcgtacgcg caaggctgtg
     8041 tccagcggtc gggccgctcg atcaggcact tctcgtcggt caacaacgcc cgtgacctgg
     8101 acgtcctgcg cgccgccctg ggcgagcgga agctgacctt catgggggcg tcctacggca
     8161 cgtacttcgg cacgctctac gcagagctgt tcccgtcgca cgtacgccgg atggtcctcg
     8221 actcggccgt gaacccggac ccggcgaagg tctggtaccg caacaacctc gaccagtccc
     8281 tcgccttcga gggccgctgg gccgacttcc gcgcctggat cgccaagcac cacaaggtgt
     8341 acgggctggg ccgcacgccc caggaggtgc agcgcagtta cgagcgggcg cgggcacgac
     8401 tggcggcgaa gccggcgggt ggcaaggtcg ggcccgggca gttgcggggc gcgttcttgc
     8461 aggccgggta ctacgacgac tactggccgc agcgcgcggc ggcgctctcc gcgtatctga
     8521 agggcgatcc gaagccgctg atccggcagg cggggccgca tcgggacggg gtgaaagagc
     8581 aggagaacag caaggccgtc tacacggccg tcgagtgcaa cgacgcgccc tggccgcggt
     8641 cctggcacac ctgggaccgc gacaacaccc gtctcgcgca cgcggcgccc ttcgagacct
     8701 gggacaacgt ctggacgaac ctgccgtgcg cctactggcc gctgccgcgc acgcgcccgc
     8761 tcgacgtccg tacgtcaccc ggcgagctgc cgccgacact gatcctggcc gccgaacggg
     8821 acgcggcgac gccgtacgcc ggcgcgctgg agacgcaccg gcgactgtcc ggctcgacgt
     8881 tggtcaccga gcgggcgtcg ggctcccacg gcatcggcgg cggcgcgaac gcgtgcgtca
     8941 acgcccacct ggacgcgtac ttgctacggg gccggctccc cgcacccgag gcaacctgcg
     9001 ccccgcacaa ggagccgacc ccggccccct aggggcgccc cgtcaggggc gcggggaact
     9061 gcgcgatcag cccccaccgg cgggcggcca acacacaacc gcccgcaccc ctgaaagaac
     9121 caaaaaacca aaaaacctac gcgaggccct cgaccagttc ggtcacggac ttgcgacggc
     9181 ccgtgtagaa cgggacctcc tcgcggacgt gcatgcgggc ctcggacgcg cgcagatgcc
     9241 gcatgaggtc cacgatgcgg tccagttcgt ccgcctcgaa cgcgagaatc cactcgtagt
     9301 cgccgagtga gaacgaggcg accgtgttcg cccgcacgtc cgggaatccg cgagccatct
     9361 tgccgtggtc ggcgagcatc cggcgccggt cctcgtccgg caacaggtac cagtcgtagg
     9421 accgcacgaa cggatacacg ctgatgtagt tccgcggtgt ctcgtccgcg aggaacgccg
     9481 gaacgtgcga gcggttgaac tcggccggcc ggtgcagcgc catgttcgac cagaccggct
     9541 ccagggcgcg gcccagcctc gtacggcgga agaggttgta cgcctcctgg agctggtcgg
     9601 aggtctcggc gtgccaccag atcatgaggt cggcgtcggc gcgcagcccc gagacgtcgt
     9661 acgtgccgcg gatcgtgacg tccttggcgg tgagctggtc gaacagctcc tggacctcgt
     9721 ccgcgtagcc ggcgcgcgcg gcgccgtcgg gaagcacgtc gcgcagcttg aacacggacc
     9781 agagggtgta gcggatgacc tcgttgaggt ccttggcctt cttgccggcg ttcggagtct
     9841 tggtcctgtc ggcctggccg gtgatggggg cgtcgtcgct catgaggcta ttctcccgct
     9901 ccgccgtgca ggctccgcac cgggtgggcg ccgagcgcgt ccgacgaccg gtcagcgtcc
     9961 gccgaccggt cgtcgccccg cagtccgtcc acggcggcgt tcgcgctcgc gacgcaggcc
    10021 gggatgccca caccgtcgta cgccgccccg cacacggcga gccccggcag cctgccgacg
    10081 tgttcgcgga tgcgggccac gcgcgcatgg tgcccgaccg ggtactgggg cagtccgtcg
    10141 ttccagcggg tcacccgggt ctccagaggt tccgcggtga ggccggtggc cgcacgcagg
    10201 tcgtgccgtg acacgtcgac gaggtccgcg tcccggcggc cgaggatctc cgtctccccg
    10261 tgccggccga cggacgtccg cagaaccgcc acgtccgggt tctcctcggc gatccagccc
    10321 catttccggg acgcgaacgt ggacgccttg atggtgcgcc cgtcgacggg cggcaccagg
    10381 aaaccgctgc cttcggggag tgtgatctcg ctgcggcggt aggcgagggt gatcagtgcc
    10441 atggacgcgt actcgatcgc cgcgagctcg gccgcggcgg cgggggcctc ggcggtgagc
    10501 agcgcggcag cggcggcagc gggcaccgcc acgacgacgg cgtcggcccg cagttcacgg
    10561 ccgcccgcga cgagccgcca gcccgcgcgc cgctcggcac cggccgcgtc gcccgcgacc
    10621 cggcgcagct ccgtgaccgg cgctcccgtg acaatctcgc cgccccgggc acgtacggac
    10681 tcggcgacgg cgagcggcag ttggccgaca ccgccctcga tgcccatgaa aaccggcccc
    10741 ggctgctgat tacgggtggt cttcgcctgg atctcgcgga ccgcctccgt gagggaggtg
    10801 tgcgtacggg ccgcctcgaa gagctgggga acggccgagc gcatcgagat gcggtacgcg
    10861 tctcccgcgt agacgccacc gagcaggggc tcgacgagac ggtcgacgac ctcacggccc
    10921 aggcgctccg cgacgaaccc gcccaccgac acgtcgtcgc cgatctgcgt gcggggcagg
    10981 tcgcggtcgc gctcgatccg ccgcaggcct tcgtcggaga ggacgccgga gagggccgcc
    11041 gcgtcccccg gaacccccat gacgtgcccc ttgggcatgg gcctgagtgc cccgcgcgtc
    11101 cagatcgagg cgcccgccgt cgccggcgac cggagccggt cggcgaggcc cacctcatgg
    11161 gcgagcgcca ccgcctcggg ccgtcgcgcg agcatcgact cggcgccgag gtcgacacgg
    11221 gcgcccgcga tcgagcccgg cagcagcttg ccgccgagcc ggtccgaggc ctccaggacg
    11281 gtcacgcgcg cgccaccgtt caggagccgg tggg
//
