LOCUS       NZ_AP023440            12910 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces aurantiacus strain JCM 4677 chromosome.
ACCESSION   NZ_AP023440
VERSION     NZ_AP023440.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces aurantiacus
  ORGANISM  Streptomyces aurantiacus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 9428393)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye
            Genome Coverage                   :: 62X
            Sequencing Technology :: Illumina HiSeq X Ten; PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014701115.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 05:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,211
            CDSs (total)                      :: 8,122
            Genes (coding)                    :: 7,928
            CDSs (with protein)               :: 7,928
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 69 of 194
            Pseudo Genes (incomplete)         :: 161 of 194
            Pseudo Genes (internal stop)      :: 17 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:48:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1678252
            Orig. end                         :: 1691162
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023440.1.
            Genome sequencing and analysis were conducted under the Global
            Catalogue of Microorganisms (GCM) 10K type strain sequencing
            project (http://gctype.wdcm.org). The genomic data are also
            available at http://gctype.wdcm.org/sequencing/GCM10017557
            Annotated by DFAST v.1.2.7 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12910
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="10"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12910
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12910
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7910](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7910)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(1251..2087)
                     /locus_tag="IGS71_RS06830"
                     /old_locus_tag="GCM10017557_14080"
     CDS             complement(1251..2087)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071330.1"
                     /locus_tag="IGS71_RS06830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14080"
                     /product="M55 family metallopeptidase"
                     /protein_id="WP_055513006.1"
                     /transl_table=11
                     /translation="MVKILISADMEGATGVTWPADVLPGTPQWERCRSMFTSDVNAAAL
                     GFFDGGADEVLVNEAHSTMRNLLLEQLDERVQMITGRHKALSMVEGVQHGDVDGIAFVG
                     YHAGAGMEGVLAHTYLANSITGVWVDGERASEGLLNARVVAEYGVPVVLVTGDDVACED
                     ALGYAPGALKVAVKDHVSRYAAVCRTPARTAADIRSAAKEAARLAVRHEPERAGPFTVA
                     LEFDAEHLSMAATVVPGVERTGERKVAYTSPTMYEGIRTFKAVTTIVDAAVEETYG"
     gene            complement(2159..2869)
                     /locus_tag="IGS71_RS06835"
                     /old_locus_tag="GCM10017557_14090"
     CDS             complement(2159..2869)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118951.1"
                     /locus_tag="IGS71_RS06835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14090"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_190849706.1"
                     /transl_table=11
                     /translation="MAAQRTSAPALPTLGGKKPSYRESVADALRAALIAGELRPGEVHS
                     APALATRFGVSATPVREALLDLAKEGLVDTVPNKGFRVTAVSEKQLDEYTHIRSLIEIP
                     TTVGLATSADPAALEALRPVAQEIVTAAAAGDLIAYVEADIRFHLGLLGLAGNAHLVEV
                     VGDLRKRSRLYGLHALVEAGRLEASAEEHLEILDALVAGDEEAVRAVMTRHLGHVRGLW
                     AAEDSAAGTPPTTT"
     misc_feature    2896..2910
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.54"
                     /tool="antismash"
     misc_feature    2903..2912
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     gene            complement(2988..3989)
                     /locus_tag="IGS71_RS06840"
                     /old_locus_tag="GCM10017557_14100"
     CDS             complement(2988..3989)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358096.1"
                     /locus_tag="IGS71_RS06840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14100"
                     /product="proline racemase family protein"
                     /protein_id="WP_055513009.1"
                     /transl_table=11
                     /translation="MRSKLVLHAVDSHTEGMPTRVITGGIGTVPGATMNERRLYFREHR
                     DDIKQLLMNEPRGHAAMSGAILQPSTRPDCDYGVIYIEVSGYLPMCGHGTIGVATVLVE
                     TGMVEVVEPVTTIRLDTPAGLVVAEVEVRDGAAGPVTLRNVPSFSVALDRKIELADGRT
                     VTYDLAYGGNFYAILPLDQFGLPFERARKDDILAAGLSLMDAINAEGEPVHPEDPSISG
                     CHHVHLIAPGSTARHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLNTEFLNES
                     FIGTHFTGRLHGTTEVAGIPAVLPSFTGRAWVTGTAQYLLDPSDPFPAGFVL"
     misc_feature    4049..4064
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.55"
                     /tool="antismash"
     gene            complement(4061..4966)
                     /locus_tag="IGS71_RS06845"
                     /old_locus_tag="GCM10017557_14110"
     CDS             complement(4061..4966)
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525029.1"
                     /locus_tag="IGS71_RS06845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14110"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_055513046.1"
                     /transl_table=11
                     /translation="MTDNRPWRGVLVATALPLKDDLSVDHDAYADHCAWLVANGCDGVV
                     PNGSLGEYQVLTPEERAKVVETAVAAIGGSRVMPGVAAYGSAEARRWAEQARDAGCAAV
                     MLLPPNAYRADERSVLAHYAEVAEAGLPIVAYNNPIDTKVDLVPELLAKLHGEGYIQGV
                     KEFSGDVRRAYRIAELAPDLDLLIGADDVLLELAVAGAKGWVAGYPNALPAASVELFRA
                     AVRGDLDTALPLYRQLHPLLRWDSQVEFVQAIKLSMDIVGRYGGPVRPPRVPLRPDQEA
                     TVRAATEKAVAAGLAAGDVV"
     misc_feature    complement(4993..5003)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            complement(5001..6434)
                     /locus_tag="IGS71_RS06850"
                     /old_locus_tag="GCM10017557_14120"
     CDS             complement(5001..6434)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656536.1"
                     /locus_tag="IGS71_RS06850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14120"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_190849707.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5.7e-27, bitscore:
                     93.6, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 3.3e-11, bitscore:
                     42.3, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDDRRGTVEPRGTTDRPRLAVIGAGPAGLAAALAAAARGVQVTL
                     VDAAPEAGGQFYRRPAAGLGARRPRALHHQWRTWERLRDGLAAHIEAGRVTHLTDHHVW
                     CVERESGVFVVHALLGPEQEEPVAVRADAVVLATGGYEKVLPFPGWTLPGVVTAGGAQA
                     MLKSGLVLPGRTAVVAGTGPLLMPVAVGLAAAGAKVAALVESADPRAFVRHARVLAAHP
                     GKVAEGALYAAGLARHRIGVHVRHAVVEAHGTDRLEAVTVAALDTDGRVRDGSEWRVPC
                     DSLAVGHGMLPHTDLAETLGCRLDGVNVAVDDEQRTDVPGVWAAGETTGIGGAVLSLAE
                     GRIAGLSVAARLDSRAPEPSSYARPANTRAGLREFFAAVDNVCTPPAGWAERIGDDILV
                     CRCEEVPASAVREAVDELGAGDVRTVKLLTRAGMGWCQGRMCGPAVAGLAGCPTTPSRR
                     PFARPVPLGVLAREPGESP"
     gene            complement(6427..6735)
                     /locus_tag="IGS71_RS06855"
                     /old_locus_tag="GCM10017557_14130"
     CDS             complement(6427..6735)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069841.1"
                     /locus_tag="IGS71_RS06855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14130"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_055513014.1"
                     /sec_met_domain="Fer2_4 (E-value: 4.5e-25, bitscore: 86.4,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPRTPMRLVRARPEPPFTVTFDDREIAVLPGQTIAAALWSVGVM
                     SWRTTRGSGERRGVFCGIGVCFDCLLTVNGRPNQRACLVRAEPGDVIRTQEGTGHHD"
     gene            complement(6732..7910)
                     /locus_tag="IGS71_RS06860"
                     /old_locus_tag="GCM10017557_14140"
     CDS             complement(6732..7910)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996369.1"
                     /locus_tag="IGS71_RS06860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14140"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_190849708.1"
                     /sec_met_domain="DAO (E-value: 7.8e-61, bitscore: 205.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNSLTCDVVIVGAGMVGAASALYAARAGLSAVLVDRGPVAGGTT
                     GAGEGNLLVSDKEPGPELDLALLSGRLWAGLAAEPGVAGSVEYEAKGGLVVAAGPEGLT
                     ALEGFAAAQRSAGVEAESVAAGQLHDLEPYVARGLAGGVLYPQDAQVMPTLAAARLVRL
                     ARTAGASLRTGWTVTEVLRTASGAVRGVRTPHGDIHAPAVVNAAGTWGGELAALAGVSL
                     PVLPRRGFVLVTEPLPRRVRHKVYAADYVADVASDSAALQTSPVVEGTAAGPVLIGASR
                     ERVGFDRSFSLPVTRELAAGATRLFPFLAEVRAMRAYLGFRPYLPDHLPAVGPDPRVPG
                     LFHACGHEGAGIGLATGTGHLIAQVLTDRTPDLDLAPFRPDRFPDPSPEEGS"
     misc_feature    7912..7928
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.15"
                     /tool="antismash"
     misc_feature    7916..7926
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 17.67"
                     /tool="antismash"
     gene            8097..8900
                     /locus_tag="IGS71_RS06865"
                     /old_locus_tag="GCM10017557_14150"
     CDS             8097..8900
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1292: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056299.1"
                     /locus_tag="IGS71_RS06865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14150"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_079103516.1"
                     /transl_table=11
                     /translation="MTDAGLDAHGIDTSKAQSARMYDYFLGGKDNYPVDWEAAEKVISF
                     FPAVQEMARVNRDFMHRAARLLAERGVRQFLDIGTGIPTAPNLHQIVQDITPDARVVYA
                     DNDPIVLRHAEALLHSRPEGRTTYIQADVRDPGSILAAAREVLDFEQPVALSLVALLHL
                     VADDDEPHRIMSELRGSLAAGSHVALSHATGDFDPETWERVVEVYRKGGTPAQVRSRGE
                     FSRFFEGLELLAPGIELAEHWHPELGEQRGPGERIPLYVGVARVP"
     misc_feature    8295..8297
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8944..10848)
                     /locus_tag="IGS71_RS06870"
                     /old_locus_tag="GCM10017557_14160"
     CDS             complement(8944..10848)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056484.1"
                     /locus_tag="IGS71_RS06870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14160"
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="WP_190849709.1"
                     /transl_table=11
                     /translation="MAARHRSLLGRPPAPFLVPFPVPFLALVLALFLALTGLVALPAPA
                     HAATPVCALVCDTLDPSKAQQDTFPVPNRTINGRILRLHVSDPDNTAWASIDNGVAGDA
                     VWLDRSWDRGATWDGLLGKASVPGSWTGTRTLMYNITDPVAHRRGWIRACGDAAAVTCT
                     DWVYPKVCDGASCDGADPATAAGDDTPVPATTLFGRTISLHVDQRGGMAWGVIAGGGAG
                     DEVWLDRSWDSGASQPEGSSLGRKSVPSGATTTRTVMFATRDPRGLLYGGAVRVCGREA
                     SHQEGSCTAWARPAPTRARGAADALMASYRTDEGWWRSSWWNSAVALTSLIDFAEQSGR
                     HDYDWAIARTFEQNRGVFPAGGRSSDPIEGHFISRAIDDAGWWAMAWLKAYDYTGDRRY
                     LEEAVTITTYVHQYWDTGTCGGGVWWDRERTYKNAVTNGQYLWLTTALHQRISGDTVWG
                     ARATTAANWYLASGMINSSGLVNDGITTGCANNGQTVWSYNQGLAIGAFTQLWRSTGNS
                     RYLDTAERLADSAISSPALTRGGVLTESCDISTATCDDNQKQFKGIFIRHFADLADATA
                     STTYKAYVTKQADSIWTTDRDPLNRLGARWSGTSPNTSDWRTQASALEALTAAGRLLD"
     gene            complement(11057..11779)
                     /locus_tag="IGS71_RS06875"
                     /old_locus_tag="GCM10017557_14170"
     CDS             complement(11057..11779)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431731.1"
                     /locus_tag="IGS71_RS06875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_14170"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_190849710.1"
                     /transl_table=11
                     /translation="MSVTTRYREAWDGFWSEASAEQGAVFWDADPALTAGVHLALFEPY
                     LAAPGLPLVDLGCGNGTQTRFLADRYPRVVGVDLSAAALDHARLADLAGQASYRLFDAA
                     EKAGAERLHAELADANVYMRGVLHQCEPADRQPLVDGLAALVGDRGRVFLVEPAEAAGA
                     VLRALARSPGGPPAKLASVLRHGIAPGEVADAAVPEYLRAAGLTVLASGELPLVTSERR
                     ADGSRIELPSLWVVAGRG"
ORIGIN
        1 cgtagccggt gatgcccagg cgcgagaact gcttggcgtc cgtgccgccc gacatgcagt
       61 agggcacgac gttcccctcg ggcgcgaact cctcgacggc ggcccgcatc cgggcgtacg
      121 tcgtcgagtc cagcggcgcc tggagggcga cctcgcggtg ggcgaactcc cactccacgt
      181 ccggcccggt gagttcgtcg agggtggccc ggaactcgtc ctcggcaccg gccaggaagc
      241 gtccgtcgat gtgggcgacg gcctccccgg gaatcacgtt gaccttgtaa ccggcgtcga
      301 gcatggtcgg gttggcgctg ttgcggaccg tggcctcgac gagggaggcg gcgggaccga
      361 gcttcgccat cagcgcgtcc acggcgtcga gagcaccgag gccaccgaga ccaccgagtt
      421 cgctggggcc gtcggggtcg ccgaggtcgg gctcgatgcc gtacagcagg gcgagttcgg
      481 tgagcgccgc gcggaccgtc ggggtgagcc gtacgggcca ccggtgggcg ccgatccgcg
      541 cgacggcggc ggccagccgg gtcaccgcgt tggaccggtt caccttggag ccgtggcccg
      601 cccggccgcg ggcggtgagc ttcagccagc cggtgccgcg ctcgccggcc gcgatcgggt
      661 acagctcgcg gccaccgccg tcgtggaagg tgaaggcccc ggactcgctg atgccctcgg
      721 tgcacccctc gaagagcccc gcgtgccggt cggcgaggaa gcccgagccg tcctcggcgc
      781 tcgcctcctc gtccgcggtg aacgcgatca ccaggtcccg acgggggcgt acaccggagc
      841 gcgcccactg ccggacgacc gcgaggatca tcgcgtccat gttcttcatg tcgacggcgc
      901 cccgccccca gacgatcccg tcacggacct cgccggagaa cggatcgacg ctccagtcct
      961 cggcctgcgc gggcacgacg tccaggtgac cgtggacgag cagcgcgtcg gcggacgggt
     1021 cggtgccctc caggcgcgcg acgacgttcg tacgcccctt ggtgcgctcc agaagggtcg
     1081 gctgcaggcc ggcctcggcg agccgtgcgg cggcgtactc ggcggccggc cgctcctggc
     1141 agtcgccgcc accgcggttg gtggtgtcga tccggatgag atcggaggtg aaccggacga
     1201 cctcgtccag cgcctcctcg tcgacggccg cctgctcgat cacgctcatg tcagccatag
     1261 gtctcctcca cggcagcatc gacgatcgtg gtgaccgcct tgaaggtccg gatgccctcg
     1321 tacatggtgg ggctggtgta cgccaccttc cgttccccgg tgcgttcgac gcccgggacg
     1381 acggtggccg ccatcgagag gtgctcggcg tcgaactcca gggccacggt gaaggggccc
     1441 gcccgctcgg gctcgtgccg caccgcgagc cgcgccgcct ccttggccgc cgagcggatg
     1501 tccgccgcgg tacgggccgg agtgcggcac acggccgcgt accgcgagac atggtccttg
     1561 acggcgacct tcagcgcccc gggcgcgtag ccgagcgcgt cctcgcaggc cacgtcgtcc
     1621 ccggtgacca ggacgacggg gaccccgtac tcggcgacga cgcgcgcgtt gagcagtccc
     1681 tcactggcgc gctcgccgtc gacccacaca ccggtgatcg agttggcgag gtaggtgtgg
     1741 gcgaggacgc cctccatgcc cgctcccgcg tggtagccga cgaaggcgat gccgtcgacg
     1801 tccccgtgct gcacgccctc caccatggag agcgccttgt gccgcccggt gatcatctgc
     1861 acgcgttcgt ccagttgttc caggagcagg ttgcgcatgg tcgagtgggc ctcgttgacg
     1921 aggacttcgt cggccccgcc gtcgaagaag ccgagggccg cggcgttcac gtccgaggtg
     1981 aacatcgacc ggcaccgctc ccactgcggg gtgcccggca gcacgtcggc cggccaggtc
     2041 accccggtgg cgccctccat gtcggcgctg atcaggatct ttaccatgct ccagttgtat
     2101 cgcagtcgca cggcgcccca cagggccagg ggtcagctgg acgaaccggc gtaaaccttc
     2161 atgtggtcgt cggaggcgtg ccggcggcgg aatcctcggc cgcccacagt ccccgtacgt
     2221 gccccagatg ccgtgtcatg acggcgcgta ccgcctcctc gtcgccggcc acgagtgcgt
     2281 ccaggatctc caggtgctcc tcggcggacg cctccaggcg gcccgcctcg accagggcgt
     2341 gcaggccgta gaggcgggaa cgcttgcgca ggtcgccgac gacctcgacc aggtgcgcgt
     2401 tgcccgcgag gccgagcagg ccgaggtgga agcggatgtc cgcctcgacg tacgcgatga
     2461 ggtcgcccgc cgccgcggcc gtgacgatct cctgggcgac gggtcgcagc gcctccaggg
     2521 cggcggggtc ggcggaggtg gcgaggccga cggtggtggg gatctcgatg agcgagcgga
     2581 tgtgcgtgta ttcgtcgagc tgcttctcgg agaccgcggt gacccggaag cccttgttgg
     2641 gcacggtgtc gaccaggccc tccttggcga ggtcgagcag ggcctcgcgg accggggtgg
     2701 cggagacgcc gaagcgggtg gcgagggccg gtgcggagtg cacctcgccg gggcgcagct
     2761 ctcccgcgat gagggcggcc cgcagcgcgt ccgcgacact ctcgcggtag ctgggcttct
     2821 tgccgcccag cgtgggcagg gcgggggcgc tggtgcgctg ggcggccatc ggtcggtctc
     2881 ctcggagggg gtggctgggc gtcatttttt gggggcgcgg ggaactgcgc aatccttgag
     2941 ccgcgcggga caggcgggac aggcggacag gcgggacaca caggggacta caggacgaag
     3001 cccgccggga acgggtcgct cgggtcgagc aggtactggg cggtgccggt cacccacgcg
     3061 cgcccggtga agctgggcag cacggccggg attccggcga cctcggtggt gccgtggagg
     3121 cgccctgtga agtgggtgcc gatgaatgac tcgttcaaaa actcggtgtt caggggcagt
     3181 tcaccgcgcg cgtgcagttg cgccatgcgc gcgctggtgc ccgtgccgca gggcgagcgg
     3241 tcgaaccagc ccgggtggat ggccatcgca tgccgcgagt gccgggccgt cgaacccgga
     3301 gcgatcaggt gcacatggtg gcagccactg atggaggggt cctccggatg gacgggctcc
     3361 ccttcggcgt tgatggcgtc catcagtgac agcccggcgg cgaggatgtc gtccttgcgg
     3421 gcccgctcga agggcagccc gaactggtcg agcggcagga tcgcgtagaa gttcccgccg
     3481 tacgcgaggt cgtaggtcac cgtccgcccg tcggcgagct cgatcttgcg gtccagggcg
     3541 acggagaagg acggcacgtt cctgagcgtg accggcccgg ccgccccgtc ccgcacctcg
     3601 acctcggcga cgacgagccc ggccggggtg tcgagccgga tggtcgtcac cggctcgacg
     3661 acctcgacca tgccggtctc cacgaggacg gtcgcgaccc cgatggtccc gtgcccgcac
     3721 atcgggagat agcccgacac ctcgatgtag atgacgccgt agtcgcagtc ggggcgggtc
     3781 gacggctgga ggatcgcgcc gctcatcgcc gcgtggccgc gcggctcgtt catcagcaac
     3841 tgcttgatgt cgtcgcggtg ttcacggaag tacagtcgcc gctcgttcat ggtcgcgccg
     3901 gggaccgtgc cgatcccgcc ggtgatgacc cgggtcggca tgccctcggt gtgcgagtcg
     3961 acggcgtgca ggacgagttt gctgcgcatg actcagtctc cctgtctcca tgagtctccc
     4021 tgtctccctg tctctgcatc tcctcgtctt cacatctctt tcagacgacg tctcctgcag
     4081 cgagtcccgc cgccacggcc ttctccgtgg cggcccggac ggtggcctcc tggtcgggtc
     4141 gcagcggcac gcgcggcggg cgcaccggac cgccgtaccg gccgacgatg tccatcgaca
     4201 gcttgatggc ctggacgaac tccacctggg agtcccagcg cagcagcggg tgcagctgcc
     4261 ggtagagggg cagcgcggtg tcgaggtcgc cccggacggc ggcgcggaac agttcgacgg
     4321 acgcggcggg cagcgcgttc gggtagccgg cgacccagcc cttggcgccc gcgaccgcca
     4381 gctccagcag gacgtcgtcg gcgccgatca acaggtcgag gtccggggcg agttcggcta
     4441 tgcggtaggc ccggcgcaca tcaccggaga actccttgac gccctggatg tatccctcgc
     4501 cgtgcagctt ggcgagcagt tcgggcacca ggtcgacctt ggtgtcgatg ggattgttgt
     4561 acgccacgat cggcaggccc gcctcggcga cctcggcgta gtgggcgagg accgaacgct
     4621 cgtcggcccg gtaggcgttc ggaggcagca gcatgacggc ggcgcagccg gcgtcgcgtg
     4681 cctgctcggc ccagcggcgg gcctcggcgg agccgtacgc ggcgacgccg ggcatgacgc
     4741 gcgagccgcc gatcgccgcc acggccgtct ccacgacctt ggcgcgctcc tcgggggtca
     4801 gcacctggta ctcgccgagc gagccgttgg gtacgacgcc gtcgcagccg ttcgcgacga
     4861 gccacgcgca gtggtcggcg taggcgtcgt ggtcgacgga gaggtcgtcc ttgagcggga
     4921 gggcggtggc gacgaggacg ccgcgccagg ggcggttgtc ggtcatgagg tttcccatcc
     4981 ggtccgtagg gcgtgacatg tcagggggat tcgccgggct cgcgtgcgag cacgccgagg
     5041 ggtacggggc gggcgaacgg ccgtcgtgag ggcgtcgtcg ggcagcccgc gagccccgcc
     5101 accgccgggc cgcacatccg cccctggcac cagcccattc cggcgcgggt cagcagtttc
     5161 acggtgcgta cgtccccggc gccgagttcg tcgacagcct cccggacagc ggaggcgggc
     5221 acctcctcgc agcggcacac gaggatgtcg tcgccgatcc gttcggccca accggcgggc
     5281 ggcgtacaga cgttgtcgac ggcggcgaaa aactcccgca gtccggcacg cgtgttggcg
     5341 gggcgcgcgt aggaactcgg ctcgggcgcc cgtgagtcga gccgtgccgc gacggacagc
     5401 cccgcgatcc gcccctcggc cagtgagagc accgcgccgc cgatcccggt ggtctccccg
     5461 gcggcccaga cgcccggtac gtcggtgcgc tgctcgtcgt ccacggcgac gttcacgccg
     5521 tcgaggcggc agccgagcgt ctcggcgagg tcggtgtgcg ggagcatgcc gtggccgacg
     5581 gcgaggctgt cgcaggggac gcgccactcg ctgccgtccc tgacgcgtcc gtcggtgtcg
     5641 agcgcggcca cggtcacggc ttcgagccgg tcggtgccgt gcgcctcgac gacggcgtgg
     5701 cgcacgtgga ccccgatccg gtgccgcgcc aacccggccg cgtacagggc gccttcggcg
     5761 accttgccgg ggtgggccgc gaggacccgg gcgtgccgta cgaacgcccg ggggtcggcg
     5821 gactccacga gggcggccac cttcgcgccg gccgcggcca gcccgacggc cacgggcatc
     5881 agcagcggcc cggtcccggc gaccacggcg gtacggcccg gcaggacgag cccgctcttg
     5941 agcatggcct gcgccccgcc cgcggtgacg actccgggga gggtccagcc ggggaacggc
     6001 agcaccttct cgtacccgcc ggtcgcgagg accacggcgt cggcgcggac cgcgaccggt
     6061 tcctcctgct caggcccgag cagggcgtgc acgacgaaga cgccggactc ccgctccacg
     6121 caccacacat gatggtcggt caggtgcgtg acgcgccctg cctcgatatg ggccgccagt
     6181 ccgtcacgga gtcgttccca ggtacgccac tggtggtgca gggcgcgggg gcggcgggca
     6241 ccgagcccgg cggcgggccg gcggtagaac tgcccgcccg cctccggggc ggcgtccacg
     6301 agggtcacct gtacgccacg cgcggcggcg gcgagggccg cggcgagccc ggcggggccc
     6361 gcgccgatca cggcgaggcg gggtcggtca gtcgtgccgc gtggttcgac tgttccgcgt
     6421 cggtcgtcag tcatggtggc cggtcccttc ctgcgtgcgg atcacgtcac cgggctccgc
     6481 acgcaccagg caagcccgtt ggttgggccg cccgttgacg gtcagcagac agtcgaagca
     6541 caccccgata ccgcagaaga caccgcgtcg ctcgccgctc ccccgggtcg tacgccagga
     6601 catgacaccc acggaccaca gggccgccgc gatcgtctgt ccggggagaa cggcgatctc
     6661 gcggtcgtcg aacgtgaccg tgaacggcgg ctccggccgg gcccggacca gcctcatggg
     6721 cgtacgcggc gtcatgagcc ctcctcgggc gacggatcgg gaaaccggtc ggggcggaac
     6781 ggtgcgaggt ccaggtcggg cgtccggtcc gtcagtacct gggcgatcaa gtggcctgtc
     6841 cccgtggcga gtccgatgcc cgcgccctcg tgcccgcacg cgtggaagag accgggcacc
     6901 cgcgggtcgg gcccgacggc ggggaggtgg tcgggcaggt acgggcggaa gccgaggtag
     6961 gcgcgcatgg cccgcacctc cgccaggaag gggaacagcc gggtcgcccc cgccgcgagt
     7021 tcccgggtga cgggcaggga gaaggagcgg tcgaagccga cccgttcccg gctggccccg
     7081 atcagtaccg gtcccgccgc cgtgccctcg accaccgggg aggtctggag ggccgccgag
     7141 tcgctggcca cgtcggccac atagtccgcc gcgtacacct tgtggcgcac cctgcgcggc
     7201 agtggctcgg tgacgaggac gaagccgcgc cggggcagca ccggcagcga caccccggcc
     7261 agcgcggcca gttccccgcc ccaggtcccg gccgcgttga cgaccgcggg cgcgtggatg
     7321 tcgccgtgcg gggtacggac accgcgcacc gcccccgacg ccgtacgcag cacctccgtc
     7381 accgtccagc cggtgcgcag cgaggcaccc gccgtgcggg ccagccgcac gagccgggcc
     7441 gccgcgaggg tcggcatcac ctgggcgtcc tgcggataga gcacgccacc cgcgagcccc
     7501 cgcgccacgt acggttcgag gtcgtggagt tggcccgcgg cgacggactc ggcctcgact
     7561 ccggccgagc gctgtgcggc ggcgaacccc tccagcgccg tcagcccctc gggcccggcg
     7621 gcgacgacga ggccgccctt ggcctcgtac tcgaccgacc ctgccacgcc gggctcggcg
     7681 gcgaggccgg cccacagccg tccggagagc agcgcgaggt cgagttcggg gcccggctcc
     7741 ttgtcggaga cgagcaggtt gccctcgccc gcgccggtcg tgccgccggc caccggaccc
     7801 cggtccacca ggacggcact cagccccgcc cgagccgcgt acagggcgct cgccgccccc
     7861 accattccgg ccccgacgat cacgacatcg caggtcagcg agttgctcac gtcagtacta
     7921 tgtcacatac ctctgcgatc cacacccccc tgcaccgcgc acgcaagcag gcatacccct
     7981 ctgtccgcgc cacagaggcc gtgacaaact tctcggccat cgaccgtcgg ccgtcactca
     8041 tcgaccgtcg gccatcagcc gttcgagcac tttcgcagca gcatcggagg accgacgtga
     8101 cagatgcagg actcgacgca cacggcatcg acaccagcaa ggcccagtcg gcccgtatgt
     8161 acgactactt cctcggcggt aaggacaact accccgtcga ctgggaagcc gccgaaaaag
     8221 tgatctcctt cttccccgcc gtacaggaaa tggcgcgggt caaccgtgat ttcatgcacc
     8281 gggccgcccg gctgttagcc gagcgaggag ttcgtcaatt cctcgacatc ggcacgggca
     8341 ttcccacggc tccgaacctt catcagatcg tccaggacat cacgcccgac gctcgcgtgg
     8401 tgtacgcgga caacgatccg atcgtcctca ggcatgccga ggcacttctc cacagcaggc
     8461 ccgaaggacg taccacctat atccaggccg atgtgagaga tccgggaagc atcctcgcgg
     8521 cggcgcggga ggtgctggat ttcgagcagc ccgtcgccct ctccctggtg gccctgctgc
     8581 acctggtggc cgacgacgac gaaccccacc ggatcatgag tgagctgcgc ggctccctgg
     8641 cggccggaag ccatgtggcg ctctcccacg ccacagggga tttcgaccct gagacatggg
     8701 agcgcgtcgt cgaggtctac cgcaagggcg gaacccccgc ccaagtgcgg tcacgtggtg
     8761 aattctcacg gttcttcgag ggacttgagc tgctcgcccc cggaatagag ctggcggagc
     8821 actggcaccc ggagctcggg gaacagcgcg gcccgggcga gcggatcccg ttgtacgtgg
     8881 gcgtggcccg cgtgccctga tatccgatat ccgccccgaa acccacgtac cgggtgcccc
     8941 ttctcagtcc aggagtcggc cggcggccgt cagcgcctcc agcgcactgg cctgggtgcg
     9001 ccagtccgac gtgttggggc tggtgccgga ccagcgtgcg ccgaggcggt tgagcgggtc
     9061 ccggtcggtc gtccagatcg agtcggcctg tttcgtgacg tacgccttgt aggtggtcga
     9121 ggccgtggcg tccgccaggt cggcgaagtg ccgtatgaag atgcccttga actgcttctg
     9181 gttgtcgtcg caggtggccg tggagatgtc gcaggactcg gtcagtacgc cgccacgggt
     9241 cagcgcgggt gaggagatcg cgctgtcggc caggcgctcg gccgtgtcga ggtaccggct
     9301 gttcccggtc gaccgccaca gctgggtgaa ggcgccgatg gccaggccct ggttgtaact
     9361 ccacaccgtc tggccgttgt tggcgcagcc ggtggtgatc ccgtcgttga cgagccccga
     9421 ggagttgatc atcccgctgg cgaggtacca gttcgccgcc gtggtcgcgc gggcacccca
     9481 cacggtgtcg ccgctgatcc gctggtgcag tgcggtggtg agccacaggt actgcccgtt
     9541 ggtcacggcg ttcttgtagg tgcgctcccg gtcccaccac acaccgccgc cgcaggtacc
     9601 ggtgtcccag tactggtgga cgtaagtggt gatcgtgacc gcttcctcca gatagcgccg
     9661 gtcgcccgtg tagtcgtacg ccttcagcca ggccatcgcc caccagcccg cgtcgtcgat
     9721 ggcgcggctg atgaagtggc cctcgatcgg gtccgaactg cgcccgcccg ccgggaagac
     9781 gccccggttc tgttcgaagg tgcgggcgat cgcccagtcg tagtcgtgcc ggccgctctg
     9841 ctcggcgaag tcgatgagcg aggtgagggc gacggcggag ttccaccaac tgctgcgcca
     9901 ccagccctcg tcggtgcggt acgacgccat cagcgcgtcc gccgccccgc gcgcccgggt
     9961 cggagcgggc cgggcccacg ccgtgcaact gccctcctgg tgcgatgcct cacgtccgca
    10021 gacccggacc gctccgccgt acagcaggcc gcgtgggtcc cgggtggcga acatgacggt
    10081 gcgggtggtg gtggctccgg agggcacact tttacggccg agggaggagc cctccggctg
    10141 gctcgcgccg ctgtcccagg agcggtccag ccagacctcg tcgcccgcgc cgccgcccgc
    10201 gatgacgccc caggccatac cgccccgctg gtcgacgtgc aggctgatgg tgcgtccgaa
    10261 gagggtggtg gcgggcaccg gggtgtcgtc accggccgcg gtggcggggt cggcgccgtc
    10321 gcaggacgcg ccgtcgcaca ccttcgggta cacccagtcg gtgcaggtca ccgcggcggc
    10381 gtcgccacag gcgcggatcc agccacgccg gtgcgcgacc gggtcggtga tgttgtacat
    10441 cagggtgcgg gtgccggtcc acgagccggg gacgctcgct ttgccgagca ggccgtccca
    10501 ggtggcgccc cggtcccagg agcggtccag ccagaccgcg tcaccggcca cgccgttgtc
    10561 gatgctcgcc caggccgtgt tgtccgggtc ggagacgtgc agccgcagga ttctgccgtt
    10621 gatggtcctg ttcggcaccg ggaacgtgtc ctgctgggcc ttcgaggggt cgagggtgtc
    10681 gcacaccagg gcgcagaccg gcgtcgcggc atgggcaggg gcgggaaggg cgacgaggcc
    10741 ggtcagcgcc aggaacaggg ccagaaccag cgcgaggaac gggacaggga acgggacaag
    10801 gaacggggcc ggcggtctgc cgaggaggga gcggtgccgt gctgccatga cgtatcgcct
    10861 ctctggagaa gccgtgcacg gaagggcagc ggcggccggc cgagacgggc agccaccacg
    10921 atgagcaccc gcgagcccag ggtgcagcga tcgcccaggt gatgtctttc cccaatcaag
    10981 acgtgcccat gtcaagaacg tcccgcgtcg aggacctggg acgtattcat gccgcgaacg
    11041 tccggcgtcg acggcctcag ccgcgccccg ccaccaccca cagcgacggc aactcgatcc
    11101 gtgagccgtc ggcacgacgc tccgaggtga cgagcggcag ctccccgctc gccaggaccg
    11161 tcagccctgc cgcacgcaga tactccggta cggccgcgtc ggccacctca cccggtgcga
    11221 tgccgtgccg caggacggac gcgagtttcg ccggcgggcc accggggctc cgggcgagag
    11281 cgcgcagcac ggcgccggcc gcctcggcgg gctcgacgag gaacacgcgg ccccggtccc
    11341 cgacgagcgc ggccaggccg tcgacgagcg gctgccggtc ggccggctcg cactggtgca
    11401 ggacgccccg catatagacg ttggcgtcgg cgagttcggc gtgcagcctc tcggccccgg
    11461 ccttctccgc cgcgtcgaac agccggtagg acgcctggcc cgccaggtcc gcgagccggg
    11521 cgtggtcgag ggccgcggcc gacagatcga cgccgacgac ccgcggatag cggtcggcga
    11581 ggaaccgggt ctgggtgccg ttgccgcagc cgaggtccac gaggggcagc ccgggggcgg
    11641 ccagatacgg ttcgaacagg gcgagatgca cgcccgcggt gagcgccgga tcggcgtccc
    11701 agaagaccgc cccctgctcg gcggaagcct cgctccagaa gccgtcccag gcctctctgt
    11761 accgagtggt gacgctcata ccggctcccc aggatgcgac cgcgaaccgc cgaaaagtga
    11821 cggacagtcc ggtctaccgc gcccgaagca caccgacaag aacacggcac acaccttcaa
    11881 gccccacagg ggggcgcagc cccccaaggg gcgcggggaa cggcgcgccc agcccccacc
    11941 gaacccgcag ccaatcaacc acggacccag cggagcgcta ccgcatcgcc agctcgaacc
    12001 acaccgtctt gcccgagttc gtccggctgg tcccccactc ccgcgccagc ctgctgacca
    12061 cgcgcatccc ccgcccggcc tcgtcgagcg ggcccgcgct gagcagcgtc ggcagggtgt
    12121 ggtcgtcgtc gtccacctcg cacagcagcg tttcgccccg gaccagcctg aggtcgatcg
    12181 gccggctgtg ggagtgccgt acggcgttgg tgacgagctc gctcaccagc gactcggtga
    12241 tgtcggcgag ggaggtgagg ccccacacgt ggagctgttc gcggaccacc gcacgcgccc
    12301 ggccgacctc ctgcgggtcg tgccctatcc gccactgggc gacgtcctcg ggctcgatgc
    12361 cgttgagcct ggccagcagg agcgccacgt cgtccttgcg tccgccgcgc cccgcttcgg
    12421 agaacgtcac agccagggcg cggatgatgg tgtcgcaggc gtcgtccatg gacgcggccg
    12481 ggtgggccgc ggactcgcac agggtcgcga ggccgacacc gatgtcctca ccgcgcacct
    12541 cgacaagacc gtcggtgcac atcacgagcc ggtcgccggg cgccacccgt acgcgtacgg
    12601 actcgaaggg caccccgccg acgccgatgg gcgcgcccgt cggcaggtcg agcagatcac
    12661 tccggccgcc ctcggcgcgg accagcacgg gcgggatgtg gcccgcgttg gcgatctgca
    12721 gctcgctcgc gatggggtcg tagatcgcgt acagacaggt cgcgaggtag tgctcgccca
    12781 gtcgctgtgc caggtcgtcg aggttgcgca gaagttgggc gggcggcagg tcgagggccg
    12841 ccatggtctg tacggcggtg cgcaactgcc ccatcatcgc ggccgagttg agaccgtgcc
    12901 ccatgacgtc
//
