LOCUS       NZ_AP023440            22084 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces aurantiacus strain JCM 4677 chromosome.
ACCESSION   NZ_AP023440
VERSION     NZ_AP023440.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces aurantiacus
  ORGANISM  Streptomyces aurantiacus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 9428393)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye
            Genome Coverage                   :: 62X
            Sequencing Technology :: Illumina HiSeq X Ten; PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014701115.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 05:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,211
            CDSs (total)                      :: 8,122
            Genes (coding)                    :: 7,928
            CDSs (with protein)               :: 7,928
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 69 of 194
            Pseudo Genes (incomplete)         :: 161 of 194
            Pseudo Genes (internal stop)      :: 17 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:48:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1561427
            Orig. end                         :: 1583511
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023440.1.
            Genome sequencing and analysis were conducted under the Global
            Catalogue of Microorganisms (GCM) 10K type strain sequencing
            project (http://gctype.wdcm.org). The genomic data are also
            available at http://gctype.wdcm.org/sequencing/GCM10017557
            Annotated by DFAST v.1.2.7 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22084
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="9"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22084
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22084
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12084](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12084
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     gene            189..1052
                     /locus_tag="IGS71_RS06315"
                     /old_locus_tag="GCM10017557_13030"
     CDS             189..1052
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018524949.1"
                     /locus_tag="IGS71_RS06315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13030"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_190849658.1"
                     /transl_table=11
                     /translation="MTGRPEPSTDGTDGTEEVSDLFRAVGRQIKTARERAGLSQKELGR
                     RVGYGEEQISSVERGRRTPQPEFLAAVDELLGCDGLLAVTAEDVERAKARRRVRHPEWF
                     RDYARLEKEAVELHDYSSLVLPGLLQTEAHARVVFGTRQPLLDEVTVEQRVAARLDRQG
                     IFERWPPPFTTFTIEESVLRRPLGGWDVHHEQLEALLRFGTLRSVELQVLPMEREEHPS
                     LGGPFIMLTPRDKPQVAYLEVQHISRLITDPTDVRILAARYGSIRAQALTPGESRTLIK
                     KMLGDQ"
     gene            1049..1252
                     /locus_tag="IGS71_RS06320"
                     /old_locus_tag="GCM10017557_13040"
     CDS             1049..1252
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004980021.1"
                     /locus_tag="IGS71_RS06320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13040"
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_190849659.1"
                     /transl_table=11
                     /translation="MNSEQLARLDWFKSSYSSGEGGACVEVAADRAAIRVRDSKKHTGP
                     QLAFTRAAWAGFVTDLGRDPSH"
     gene            1312..2427
                     /locus_tag="IGS71_RS06325"
                     /old_locus_tag="GCM10017557_13050"
     CDS             1312..2427
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656984.1"
                     /locus_tag="IGS71_RS06325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13050"
                     /product="DUF5954 family protein"
                     /protein_id="WP_220450578.1"
                     /transl_table=11
                     /translation="MSDDWKRRMERAAAELLRQDDPVALVREADALDASTRCPYVWPRG
                     PVFGVAALGPEPGARWRLVSEATDGTPQEARDALNSLLWLRAKDEAETVAERRELLAAV
                     RRLECERIDELTVLGSRYRVVRGDEYARCSLTDGPEPPRPSDPEPADLSWGHQPGRDRD
                     LPDIGHLLDTRPDDPAAPFGVMAEGNRQALREFTYEGEHVPPAMRAESQQAVHRYPELI
                     LLPVGFGVIKRAGAGWTPASRLQPTPHDARRVVHNGLSWLWAEMYELDTATREAYTRAA
                     EDLEATPHANEVTVLGDTYRICRVERMLRTGPDGPEPPRSSDPENTEPMRIRPPMDEHG
                     NIQPESEPEPESESDSMSAGVGDGPDGGRSH"
     gene            complement(2503..4188)
                     /locus_tag="IGS71_RS06330"
                     /old_locus_tag="GCM10017557_13060"
     CDS             complement(2503..4188)
                     /EC_number="1.1.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671047.1"
                     /locus_tag="IGS71_RS06330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13060"
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="WP_055512010.1"
                     /transl_table=11
                     /translation="MTVEYVDAGEAIDHGALSAASGTAPAVANNVNYKRLLDARSEPEN
                     WLTYYGDYDGKRYSLLDQINTENVKRIGPAWIFQAGSSGQIAGASTYAFEACPIVVDGV
                     MYVTGWDGWFWALDAKTGEQLWRYKHAVPFDVSLCCGNVNRGCAVANGKVYFVTPNAHL
                     LALDATNGKKVWEKTTGDVRAGESATLAPLVVKDTLITGSSGGEFGVRGHIDCWDLETG
                     EHRWRCYTVPKPGEPGSETWPADGEAWARGGANHWVTGSYDPETNLYYAGTGNPAPDFD
                     GEVREGDNLYTDSVVAVDVDSGRIVWHYQFTPHDLWDYDSTMEMILFERDGKKLLAHFD
                     KNGYFFVLDRTNGQLQHVTPFVDRIDWGTITRDGRVTARKYPDKEGEPVHFYPGPAGAK
                     EWTHAAYSPRTDMFYVPVADVGATATRRRREFKEGMPYWGAAVQVDIDNMAGSVSAFDS
                     HGEEKWRWRNPHPMASSVLATAGDLVFSGTPTGEFIALDARTGDELWKFQCGSGHHGSA
                     MTYSVDGKQYVAVPVGWGGWLEGIIPGMLGAGHGDALIVFALPE"
     gene            complement(4219..4698)
                     /locus_tag="IGS71_RS06335"
                     /old_locus_tag="GCM10017557_13070"
     CDS             complement(4219..4698)
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:1901661 - quinone metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527357.1"
                     /locus_tag="IGS71_RS06335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13070"
                     /product="MSMEG_3727 family PQQ-associated protein"
                     /protein_id="WP_347342129.1"
                     /transl_table=11
                     /translation="MRRWSPMATVQERADLLNKLGLDAQNQAAIGRVLGTGSIARATER
                     ADGTMEATIRIKEDEIAWDPAILVLPHGGDVELTIINDDKNTHACLLPSNGDKKFIWLL
                     NHSKGRARLNLDGPGYYWYSSPGGNDEGRGLTAAIVVLGEVPPEARLDRPDQPRR"
     misc_feature    complement(4702..4722)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.11"
                     /tool="antismash"
     misc_feature    complement(4997..5012)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.89"
                     /tool="antismash"
     gene            complement(5009..7204)
                     /gene="katG"
                     /locus_tag="IGS71_RS06340"
                     /old_locus_tag="GCM10017557_13080"
     CDS             complement(5009..7204)
                     /EC_number="1.11.1.21"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]"
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="katG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527359.1"
                     /locus_tag="IGS71_RS06340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13080"
                     /product="catalase/peroxidase HPI"
                     /protein_id="WP_245702277.1"
                     /transl_table=11
                     /translation="MLGTRRAADRRGHPAAGGDNRDWWPNRLNLKVLTKHQPATGAMGE
                     EFDYAAEFRTIDLDALAHDIDEVLTTSQDWWPADFGHYGPLVLRMAWHCAGTYRVGDGR
                     GGASRGMQRFAPTNSWPDNRNLDKARRLLWPVKRKYGRKISWADLMVFAGNRALETMGF
                     DTFGFAGGREDVWESDEDVYWGPERTWLGDERHTGVRDLEEPLAADEMGLIYVDPQGPG
                     TLPDPVAAARAIRTTFGRMAMNDEETVALIVGGHTFGKTHGVADPGRHLAAEPEGAPIE
                     EQGLGWQNSYGTGKGPDTISSGLEGIWTPTPTTWDNSFLKTLFAYQWDVELSPAGLWQW
                     IPGDGQGRGTVPDPHDPSKTHTPRMLTTDLALREDPVYGPIARHFLENPDRLADAFARA
                     WFKLTHIDMGPVQRYLGPLVPSERLIWQDPVPEVAGELVDAADVAELKSRILVSGLSVS
                     QLVATAWASASTFRNSDKRGGANGARIRLEPQRNWDANGPETLTTVLSTLEGIQEQFNA
                     EQRTDKRVSLADLIVLGGCAAVEHAAGRAGHAVQVPFTPGRTDTTQEWTDVESFAVLEP
                     AADGFRNHVGKGNRLRAEYLLVERANQLTLSAPEMTVLVGGLRVLGANHGQSPLGVFTA
                     TPGSLTNDFFVNLLDMNTEWVANSANDAVTKTFEGRDRRSGEIRWTASRVDLVFGSNSE
                     LRALAEVYASEDAGEKFVRDFVAAWDKVMNLARFDLC"
     gene            complement(7380..8033)
                     /locus_tag="IGS71_RS06345"
                     /old_locus_tag="GCM10017557_13090"
     CDS             complement(7380..8033)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942056.1"
                     /locus_tag="IGS71_RS06345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13090"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_055512016.1"
                     /transl_table=11
                     /translation="MSRREVHSHEPAVQRVVTSGTFSLDGGVWEVSNNVWIVGDDVEVL
                     VIDAAHDADAIAAAVGDRRVVAVVCTHAHDDHVNQAPALADRYGAPIVLHPAEAPLWEM
                     TWQERTPDRAMADGDVLRAGGVELRALHTPGHSPGSTCLYAPELGTLFSGDTLFFGGPG
                     ATGRSFSSFDTIIESVRDRLLTLPPEVEVRTGHGEATWIGAEAPHLLDWVARGH"
     misc_feature    8318..8332
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.31"
                     /tool="antismash"
     gene            9073..9966
                     /gene="pqqB"
                     /locus_tag="IGS71_RS06355"
                     /old_locus_tag="GCM10017557_13110"
     CDS             9073..9966
                     /codon_start=1
                     /gene="pqqB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559515.1"
                     /locus_tag="IGS71_RS06355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13110"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="WP_190849660.1"
                     /transl_table=11
                     /translation="MWLRVLGSAAGGGFPQWNCGCPGCRAVRDGSRPCRPRTQSSIAVS
                     PDHQRWFLLNASPDIHAHVESLSAPQSSKGRTTPLAAVLLTDAELDHTLGLLLLREGRA
                     LEVHATQAAHEALYDGTSLLRTLERYCAVEWRPVVPGADVSLGDGLSYRAFDVPTTKQA
                     RFGPTSGQGRVVGYRLTDERSGRSAVYLPGLQQLTPAIREQLDDCACLFVDGTCWHDDE
                     LIRLGLAGKTAREMGHLPIDGPDGSLEQLSPLPVERKIYVHINNTNPILLEDAPERRLV
                     EKQGMEVAMDGLEIRV"
     misc_feature    9754..9756
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            10001..10693
                     /gene="pqqC"
                     /locus_tag="IGS71_RS06360"
                     /old_locus_tag="GCM10017557_13120"
     CDS             10001..10693
                     /EC_number="1.3.3.11"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR02111"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559516.1"
                     /locus_tag="IGS71_RS06360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13120"
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="WP_246596038.1"
                     /sec_met_domain="TIGR02111 (E-value: 3e-95, bitscore:
                     317.0, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHDLVEALDLVEALRAQSRRYHHQHPFHLRMNEGRLSRHQIQGW
                     VANRYYYQVNIPRKDAAILANCPDIDVRRRWIRRIHDHDGTRSGEGGIEAWLRLGDAVG
                     LTREEVQDERHVAPGVRAAVDDYVTFARTRPWPEAVASSLTELFAPDLMAQRIVALERF
                     YPWIDRDGLAYFRTRLDQAPRDAEHALEVVTAHCRSAEAQQRAVAALTFKCDLLWRMLD
                     AIGETYPE"
     gene            10665..10994
                     /gene="pqqD"
                     /locus_tag="IGS71_RS06365"
                     /old_locus_tag="GCM10017557_13130"
     CDS             10665..10994
                     /GO_function="GO:0048038 - quinone binding [Evidence IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PqqD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /locus_tag="IGS71_RS06365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13130"
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="WP_055512026.1"
                     /sec_met_domain="PqqD_RRE (E-value: 3.9e-24, bitscore:
                     83.1, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 9.4e-15, bitscore: 53.6,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 6.7e-07,
                     bitscore: 28.1, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSVKPTRSDRRAADRRRTATTSSSRPKLTSHVRLRFDAAREQHV
                     LLGPESVMVLNPTGADILSLCDGRHTVAEIVATLQDRYDGVVDDEVRRFLDRLATKRCL
                     EMSDG"
     aSDomain        10722..10982
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IGS71_RS06365_PqqD_RRE.1"
                     /evalue="1.30E-26"
                     /identifier="RREFam006.1"
                     /label="IGS71_RS06365"
                     /locus_tag="IGS71_RS06365"
                     /protein_end="106"
                     /protein_start="19"
                     /score="83.1"
                     /tool="antismash"
                     /translation="ATTSSSRPKLTSHVRLRFDAAREQHVLLGPESVMVLNPTGADILS
                     LCDGRHTVAEIVATLQDRYDGVVDDEVRRFLDRLATKRCLEM"
     aSDomain        10824..10973
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IGS71_RS06365_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="2.30E-09"
                     /identifier="RREFam002.1"
                     /label="IGS71_RS06365"
                     /locus_tag="IGS71_RS06365"
                     /protein_end="103"
                     /protein_start="53"
                     /score="28.1"
                     /tool="antismash"
                     /translation="VLNPTGADILSLCDGRHTVAEIVATLQDRYDGVVDDEVRRFLDRL
                     ATKRC"
     gene            10987..12084
                     /gene="pqqE"
                     /locus_tag="IGS71_RS06370"
                     /old_locus_tag="GCM10017557_13140"
     CDS             10987..12084
                     /GO_process="GO:0018149 - peptide cross-linking [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671044.1"
                     /locus_tag="IGS71_RS06370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13140"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="WP_190849662.1"
                     /sec_met_domain="PTHR43273 (E-value: 1.5e-29, bitscore:
                     102.0, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.5e-20, bitscore: 73.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 5.3e-06, bitscore: 25.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 6.1e-10, bitscore: 38.3,
                     seeds: 166, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 2.3e-09, bitscore:
                     36.3, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDSPFGLLAELTYRCPLSCAYCSNPVNLGDYHDELTTAQWQRVLI
                     EAQELGVLQLHLSGGEPLLRRDLVDLVTSARYLGLYTNLITSAVGLSRRRAEQLRAAGL
                     DHVQISIQADEATLSDGIAGTPSYAGKIEAARLVKELGWPLTLNVVLHRRNMDRVAAVL
                     DLAEELAADHLELANTQYYGWAWRNRDALLPSRAQLDRAEAVVRAARKRLKGRMDIVYV
                     LPDYYSQYPKPCMGGWGKRQLTVTPNGDVLPCPAAHALPLPRASVHEDSLARIWARSPL
                     FQSFRGTDWMPEPCRSCERREIDFGGCRCQAFQLTGDASRTDPVCHLSPDHGIVAERVR
                     AANEENRLGDSVLVPRPHRGRPGRR"
     misc_feature    11005..11007
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            12183..12731
                     /locus_tag="IGS71_RS06375"
                     /old_locus_tag="GCM10017557_13150"
     CDS             12183..12731
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671043.1"
                     /locus_tag="IGS71_RS06375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13150"
                     /product="manganese efflux pump"
                     /protein_id="WP_055512031.1"
                     /transl_table=11
                     /translation="MHSIWLLLGAGFVLGLDNFRVAIVLGTVPFSLRRAFQVALIFGVW
                     DGVMPLMGLLLGRYAGQAIGPISTIAASIALGGYGLYLLIQALRNPESPDEIDNPWVTL
                     FGIPLSLSLDNLVGGASLGLLGFAPWVTAIVFGAATAVMTLIGLHMGRFAAHLIRIRSD
                     LLSGVALMLAAVTLPLLPN"
     gene            complement(12818..13993)
                     /locus_tag="IGS71_RS06380"
                     /old_locus_tag="GCM10017557_13160"
     CDS             complement(12818..13993)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493195.1"
                     /locus_tag="IGS71_RS06380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13160"
                     /product="DNA-binding protein"
                     /protein_id="WP_246596044.1"
                     /transl_table=11
                     /translation="MIFTELGGLAASVAHEVDGLNGIVTVADAAGRILASWGDRATLVR
                     ASDANLAPWGCWSECAVGTNGMGTALEARGPVLIRGAEHWCRAFHDWVCAGVAVRHVVT
                     GEPIAVMNISCWRTQLPEAATRWLGNAATTAQGTLNRRARDSGAELAAAFTQAKALSGT
                     GVAAVDTAGKVVIADEPASLLMGVRPYVSAVDPDQRWDSGLPDLTRIVRYASKTAAHTP
                     DWVGSTQIYAHLRDEPTPITIRPVFLRGGLVGHLIWLGGASEGAQTLPATGPGHPWMKP
                     RRLVAVREDRTVLLRLPEVCFAESDGNNVWLFTDQGRFRSASQGLDKLDEELTDAGFLR
                     VHRRYVVNLSRVREIERGFKGELFLVMDNRKDDMVPVSRRHASAVRRALGL"
     misc_feature    14323..14336
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.3"
                     /tool="antismash"
     misc_feature    14566..14584
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.92"
                     /tool="antismash"
     misc_feature    complement(14701..14713)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.32"
                     /tool="antismash"
     tRNA            complement(14746..14830)
                     /anticodon=(pos:complement(1576222..1576224),aa:Leu,
                     seq:gag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="IGS71_RS06385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="GCM10017557_t00070"
                     /product="tRNA-Leu"
     gene            complement(14746..14830)
                     /locus_tag="IGS71_RS06385"
                     /old_locus_tag="GCM10017557_t00070"
     gene            complement(15057..16646)
                     /locus_tag="IGS71_RS06390"
                     /old_locus_tag="GCM10017557_13180"
     CDS             complement(15057..16646)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090540.1"
                     /locus_tag="IGS71_RS06390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13180"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_190849664.1"
                     /sec_met_domain="Aldedh (E-value: 7.7e-99, bitscore: 330.2,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPTPTPTSTPTPTPTPTPTLTLKSHTSWADAWQRCLAVAPEAFR
                     DDRVLNLWGAAWQADGRALPATSPVDGSPIAGPPRLDGATAQQAVRACLDHHRAWRHVP
                     LEERRARVAATLDALTQHRELLALLLVWEIGKPWRLAQADVDRAIDGVRWYVDGIEPMV
                     ADRTPLDGPVSNIASWNYPMSVLVHAMLVQALAGNAVIAKTPTDGGVACLTLACALAAR
                     EGIPVTLVSGSGGELSEALVRAPEIGCVSFVGGRDTGAAVATAVADLGKRHVLEQEGLN
                     TWGIWNHTDWDALTTVIPKLFDYGKQRCTAYPRFVVQRELFDEFLTAYLPAVDTLRVGH
                     PLAVEQPGDPYPRLDFGPVINAAKAKELHDQVAEAIDRGAVPLHRGKLADARFLPGQDT
                     SAYVQPVTILNPPPSSPLHHAEPFGPVDTIVLVDTEAELLAAMNASNGALVATLSTDDE
                     ATYSRLAPQIRAFKVGHGKPRSRGDRDELFGGFGASWRGAFVGGELLVRAVTRGPAGER
                     LPGNFPEYHLMP"
     gene            complement(16735..17634)
                     /gene="sucD"
                     /locus_tag="IGS71_RS06395"
                     /old_locus_tag="GCM10017557_13190"
     CDS             complement(16735..17634)
                     /EC_number="6.2.1.5"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="sucD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983256.1"
                     /locus_tag="IGS71_RS06395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13190"
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="WP_055512035.1"
                     /transl_table=11
                     /translation="MAIYLTKESKVLVQGMTGGEGMKHTRRMLAAGTDVVGGVNPRKAG
                     RSVDFDDRAVPVFGSVREGVEATGADVSVVFVPPAFAKAAVVEAADAGIGLAVVITEGI
                     PVHDSVAFTTYARAKGTRIVGPNCPGLITPGQSNAGIIPADITKSGRVGLVSKSGTLTY
                     QLMYELRDIGFSTCVGIGGDPVVGTTHIDCLAAFEDDPDTELIVLIGEIGGDAEERAAA
                     YVRDHVTKPVIGYIAGFTAPEGKTMGHAGAIVSGSSGTARAKKEALEAAGVQVGDTPTE
                     TARLVLARLEAARDVTHG"
     gene            complement(17646..18776)
                     /gene="sucC"
                     /locus_tag="IGS71_RS06400"
                     /old_locus_tag="GCM10017557_13200"
     CDS             complement(17646..18776)
                     /EC_number="6.2.1.5"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sucC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983257.1"
                     /locus_tag="IGS71_RS06400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13200"
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="WP_190849665.1"
                     /transl_table=11
                     /translation="MDLFEYQARELFEEHGIPVPRAEITDSSKETREIARGLGGRVVVK
                     AQVKTGGRGKAGGVKLAADPAATELTARQILGMDIKGHTVRKVMLAQPVDIDSEFYVSY
                     VLDRAAGRFLAIASAQGGMDIEEVAATRPDAVARIHIDPAVGVTSAKATEIAEAAGLPP
                     QTVDTLVRLWGVLVREDAVLVEVNPLVRTAQGQILALDGKVTLDDNARFRQARWGGQEV
                     AHDDPLEAAAAAKGLNYVKLDGEVGIIGNGAGLVMSTLDVVAGCGARPANFLDIGGGAS
                     AQIMADGLSVILSDPAVKSVFVNVFGGITACDAVADGIVRALDTVQLTKPLVVRLDGNN
                     AVRGRTILDDRAHPLVQQATTMDGAARRAAQLANAG"
     misc_feature    18821..18835
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.19"
                     /tool="antismash"
     misc_feature    18878..18896
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 23.03"
                     /tool="antismash"
     misc_feature    18891..18909
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 20.09"
                     /tool="antismash"
     misc_feature    complement(18895..18913)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 23.37"
                     /tool="antismash"
     gene            19003..20688
                     /locus_tag="IGS71_RS06405"
                     /old_locus_tag="GCM10017557_13210"
     CDS             19003..20688
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130645.1"
                     /locus_tag="IGS71_RS06405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13210"
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="WP_190849666.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 2.3e-51, bitscore:
                     172.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 9e-29, bitscore:
                     99.1, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDDNSQNLISGGHLVAKALKAEGVEVIYTLCGGHIIDIYDGCVD
                     EGIEVVDVRHEQVAAHAADGYARITGKPGCAVVTAGPGTTDAVTGVANAFRAESPMLLI
                     GGQGAHTQHKMGSLQDLPHVDMMTPITKFAATVPDTARAADMVSMAFRECYHGAPGPSF
                     LEIPRDVLDAKVPVDKARIPAAGHYRASTRSAGDPEAVEKLADLLVHAEKPAILLGSQV
                     WTTRGTESAIDLVRTLNIPAYMNGAGRGTLPPGDPHHFQLSRRYAFSNADVIVIVGTPF
                     DFRMGYGKRLSPDATVVQIDLDYRTVGKNRDIDLGIVGDAGLVLKSVTEAASGRLNGGA
                     SKRKEWLDELRAAEQTAIEKRLPSLKSDASPIHPYRLVSEINDFLTEDSIYIGDGGDIV
                     TFSGQVVQPKSPGHWMDPGPLGTLGVGVPFVLAAKKARPDKEVVALFGDGAFSLTGWDF
                     ETLVRYDLPFVGIVGNNSSMNQIRYGQAAKYGPERERVGNTLGDVHYDKFAQMLGGYGE
                     EVRDPADIGPALQRARESGKPSLINVWVDPDAYAPGTMNQTMYK"
     gene            20699..21970
                     /gene="frc"
                     /locus_tag="IGS71_RS06410"
                     /old_locus_tag="GCM10017557_13220"
     CDS             20699..21970
                     /EC_number="2.8.3.16"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_function="GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /GO_process="GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="frc"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994198.1"
                     /locus_tag="IGS71_RS06410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_13220"
                     /product="formyl-CoA transferase"
                     /protein_id="WP_190849667.1"
                     /transl_table=11
                     /translation="MTAVTTATASTPATATKALEGVRVLDMTHVQSGPSATQLLAWLGA
                     DVVKLEAPTGDITRKQLRDLPDVDSLYFTMLNCNKRSITLNTKTERGKEILTELIRRSD
                     VMVENFGPGAVDRMGFTWDRIQEINPRIVYASIKGFGDGPYTKFKAYEVVAQAMGGSMS
                     TTGFEDGPPLATGAQIGDSGTGIHAVAAILAALFQRENTGRGQRVNVAMQHAVLNLCRV
                     KLRDQQRLGHGPLAEYPNDDFGTEVPRSGNASGGGQPGWAVKCAPGGPNDYVYVIVQPV
                     GWKPLTELIGRPELAADPEWATPEARLPQLTKMFQLIEEWSATLPKWQVLEKLNAHNIP
                     CGPILSTKEIIEDESLVANEMVVTVPHPERGDFVTVGSPLKLSDSPVDVTSSPLLGEHN
                     EEVYVGELGLGDEELRLLKSNGVI"
ORIGIN
        1 agagcgtggc cgacccgacc tactctcgtg agtgacggtg cgcgtacgga gggtcactgt
       61 ccggccgtac aagtcaggct gtaccggtgg ccgacgcggt tgtacgggcg tacggccgtg
      121 gcctcgggca cccgcccatc ggtgaactcc gggcggagcg gtgaccagga cgagaacggt
      181 ggtgcggcat gacgggacga ccggagccga gcacggacgg tacggacggt acggaggagg
      241 tgtcggacct cttccgcgcg gtcggacggc agatcaagac cgcccgggag cgggccgggc
      301 tcagtcagaa ggaactgggc cgccgggtcg gctacggcga ggaacagatc tcgtcggtcg
      361 agcgggggcg ccggacaccg caaccggagt ttctggcggc agtggatgaa ctgctggggt
      421 gcgatgggct gttggcggtg acggcggagg acgtggagcg ggcgaaggcg cggaggcggg
      481 tgcggcatcc ggagtggttt cgggattatg cgcgactgga aaaggaggcc gtcgaactgc
      541 acgactacag ctccttggtg cttcctggac tcctccagac cgaggctcac gcccgcgtgg
      601 tgttcggcac acggcaaccg ctgctggacg aggtgaccgt cgagcaacga gtggctgctc
      661 gactggaccg gcaaggcatc ttcgagcgct ggcccccacc gttcacgacg tttaccatcg
      721 aagaatccgt attgcgccgc cccctcggcg ggtgggacgt ccaccatgag cagttggaag
      781 cgttgctcag gttcggcaca ctgcgcagtg tcgaactcca ggtactcccg atggaacgcg
      841 aggaacaccc cagtctggga gggccgttca tcatgctgac cccgagggac aagccgcaag
      901 tggcatatct ggaagtccag cacatcagcc gcctgatcac cgacccaaca gacgtccgta
      961 tccttgccgc acgctacggc agcatcaggg cacaggcgct cactcccggt gagtcccgaa
     1021 ctctgatcaa gaagatgctg ggagatcaat gaacagcgaa cagctcgcac ggctggactg
     1081 gttcaagagc agttacagca gtggcgaggg gggcgcgtgc gtcgaagtcg ctgccgaccg
     1141 ggccgcaatc cgcgtacggg actccaagaa gcacaccggc ccgcagctcg cgttcacccg
     1201 ggccgcgtgg gccgggttcg tcaccgacct cggccgcgac ccgtcccact gaccgcggcg
     1261 atctccggga accgtgcgac accgcacacc actcgcggcc acaatgtgtg catgagtgat
     1321 gactggaagc gacgaatgga gcgggccgcg gccgaactgc tgcggcagga cgacccggtg
     1381 gccctcgtcc gcgaggctga cgcactcgac gcctcgaccc ggtgccctta tgtgtggccg
     1441 cgcggcccgg tgttcggcgt ggccgcactc gggccggagc ccggtgcccg ctggcggttg
     1501 gtgtccgagg ccaccgacgg caccccgcaa gaggcccggg acgccctcaa ctcgttgttg
     1561 tggttgcggg cgaaggacga ggccgagacc gtcgccgagc gccgcgaact cctcgcggcc
     1621 gtgcgccgcc tagagtgcga gcggatcgac gagctcacgg tgctgggcag ccgctaccgc
     1681 gtcgtacgcg gcgacgagta cgcacgctgc agcttgaccg acggcccgga gccgccccgc
     1741 ccgtccgacc ccgaaccggc cgacctctcc tggggccacc agccgggccg cgaccgggac
     1801 ctgccggaca tcggccatct gctcgacacc cggccggacg atcccgcggc gccgttcggt
     1861 gtcatggctg aaggcaaccg ccaagcgctt cgggaattca cctacgaggg cgagcacgtc
     1921 ccgcccgcca tgcgtgccga atcccagcag gcggtccacc gttaccccga actcatcctg
     1981 ctcccggtcg gattcggcgt catcaagcgc gccggggccg gctggacccc ggccagccgc
     2041 ctccagccga ccccgcacga cgctcgccgc gtcgtacaca acggcctgtc ctggctgtgg
     2101 gccgagatgt acgagttgga caccgcgacc cgggaggcgt acacgcgcgc cgccgaagac
     2161 ctggaagcca cgccgcacgc gaacgaggtg acggtcctgg gcgacaccta ccgcatctgc
     2221 cgcgtcgaac gcatgctgcg caccggcccg gacggccccg aaccgccccg cagctccgac
     2281 ccggagaaca ccgagccgat gcgtatccgc ccgcccatgg acgaacacgg caacatccaa
     2341 cccgagtcag agcccgagcc cgagtccgag tccgacagca tgagcgcggg cgtcggcgac
     2401 gggccggacg gcgggcggtc gcactgagca gacagcaaaa gcacccgatc tcggcctgag
     2461 actgagatcg ggtgctgtca tgttgcagtc atgcggtcct gatcactccg gcagagcgaa
     2521 cacgatcaac gcgtctccgt ggcccgcgcc gagcatgccg gggatgatgc cctccagcca
     2581 gccaccccag ccgaccggca cggcgacgta ctgcttgccg tcgacgctgt aggtcatcgc
     2641 gctgccgtgg tggccgctac cgcactggaa cttccacagc tcgtcgccgg tgcgggcgtc
     2701 gagcgcgatg aactcgcccg tcggagtgcc ggagaacacc aggtcccccg cggtggcgag
     2761 caccgacgag gccatcgggt gcgggtttcg ccagcgccac ttctcctcac cgtgggagtc
     2821 gaacgcgctg accgacccgg ccatgttgtc gatgtccacc tgcacggcag caccccagta
     2881 cggcatgcct tccttgaact cacggcgccg ccgggtggcg gtggcgccca cgtcggcgac
     2941 cggcacgtag aacatgtcgg tacgcgggct gtacgccgcg tgcgtccact ccttcgcgcc
     3001 cgccgggccg gggtagaagt ggacgggctc gccctccttg tccgggtact tccgggccgt
     3061 aaccctgccg tcacgggtga tggtgcccca gtcgatccgg tcgacgaacg gggtgacgtg
     3121 ctgcagctgc ccgttggtgc ggtccagcac gaagaagtag ccgttcttgt cgaagtgagc
     3181 gagcagtttc ttcccgtccc gctcgaacag gatcatctcc atcgtggagt cgtagtccca
     3241 caggtcgtgc ggggtgaact ggtagtgcca cacgatccga ccggaatcca cgtccaccgc
     3301 gacgacgctg tcggtgtaga ggttgtcgcc ctcacggacc tcaccgtcga agtcgggcgc
     3361 cgggttgccg gtgcccgcgt agtacaggtt cgtctccggg tcgtagctcc cggtgaccca
     3421 gtggttcgcg ccgccccgcg cccaggcctc accgtcggcg ggccaggtct cggagcccgg
     3481 ctcgccgggc ttgggcacgg tgtagcagcg ccagcggtgc tcgccggtct ccaggtccca
     3541 gcagtcgatg tggccacgca cgccgaactc gccgccggag ctgccggtga tgagcgtgtc
     3601 cttgacgacc agcggagcga gtgtggcact ctcgcccgcg cgcacgtccc cggtggtctt
     3661 ctcccagacc ttcttgccgt tggtggcgtc cagcgccagc aggtgggcgt tcggcgtgac
     3721 gaagtagacc ttgccgttcg ccaccgcgca gccgcggttg acgttcccac agcagagcga
     3781 gacgtcgaac ggcaccgcgt gcttgtagcg ccagagctgc tcgccggtct tcgcgtccag
     3841 agcccagaac cagccgtccc agccggtgac gtacatgacg ccgtccacga ctatggggca
     3901 ggcctcgaac gcgtaggtcg aagcacccgc gatctggccg gaggagcctg cctggaagat
     3961 ccaggcaggc ccgatgcgct tgacgttctc cgtgttgatc tggtccagca ggctgtaccg
     4021 cttgccgtcg tagtcgccgt agtaggtgag ccagttctcc ggttccgaac gcgcgtcgag
     4081 gaggcgcttg tagttgacgt tgttggccac tgcgggcgcg gtgccggagg cggcgctcag
     4141 cgctccgtgg tcgatcgcct cacccgcgtc cacgtactcg actgtcatgg gttcgttcct
     4201 ctctaccggt ctaccggtct accggcgcgg ctggtcggga cggtcgagac gggcttccgg
     4261 cggcacctct ccgaggacga cgatggcggc ggtcaggcca cggccctcgt cgttgccgcc
     4321 cggcgagctg taccagtagt agccagggcc gtccaggttg agcctggccc ggcccttcga
     4381 gtggttcagc agccagatga acttcttgtc gccgttgctc ggcagcaggc aggcatgcgt
     4441 gttcttgtcg tcgttgatga tcgtcagttc gacgtcacca ccgtgcggca ggaccaggat
     4501 cgcgggatcc caggcgatct cgtcctcctt gatccggatc gtcgcctcca tggtgccgtc
     4561 ggctcgctcc gtggccctcg cgatgcttcc ggttccaagt acgcgaccga tcgctgcctg
     4621 gttctgtgcg tccaagccga gtttgttcag caaatcagca cgttcctgaa ctgtcgccat
     4681 tggactccac ctcctcattg cgaatccaca tgcggacgaa aatccggcgt aaccgggaaa
     4741 acgcgctgaa taccttgccg ccccatcagg cggcggcgga gcgatgcaac acacaagtcc
     4801 gagtcaatac acacatccga gccgatggcg cttcccgaat tccgatgaac cgagcaccga
     4861 tcaatgtccc gcaacgagac cggactctac gctcaccgcg gccaccccga aagggatgac
     4921 cgccggtccg gcaggcgaca ccgttcgcac ggtatgacct accgctcgga ccgatatcgg
     4981 gcgggtcggg tcgagtcgag ccagatggtc aacacaggtc gaaccgggcg aggttcatga
     5041 ccttgtccca cgcggccacg aagtcgcgta cgaacttctc tccggcgtcc tcgctcgcat
     5101 agacctccgc cagcgcacgc agttcggaat tagacccgaa gacgaggtcg acccggctgg
     5161 cggtccacct gatctcaccg cttcgccggt cacggccctc gaaggtcttc gtgaccgcgt
     5221 cgttcgccga attcgccacc cactccgtat tcatgtcgag caggttgacg aagaagtcat
     5281 tggtcaacga ccccggcgtc gcggtgaaca cgcccagcgg agactgcccg tggttggcgc
     5341 ccaggacgcg tagcccgccg acgaggacgg tcatctcggg agcgctcaac gtcagttggt
     5401 tggcgcgctc gaccagcagg tactccgccc gcagccggtt tccctttccg acgtggttgc
     5461 ggaacccgtc cgcggccggc tcaagcaccg cgaacgactc cacatccgtc cattcctgcg
     5521 tggtgtcggt acggcccggg gtgaaaggca cctggaccgc gtgcccggct ctgcctgcgg
     5581 cgtgttccac ggcagcacat ccgccgagca cgatgagatc ggccagcgag accctcttgt
     5641 ccgtgcgctg ctcggcgttg aactgctcct gtatcccttc cagggtgctc aacacggtcg
     5701 tgagcgtttc cgggccgttg gcgtcccagt tgcgctgtgg ttcgagccgg atacgcgctc
     5761 cgttcgcccc gccacgcttg tcgctgttgc ggaacgtcga ggcggacgcc caggcggtgg
     5821 ccaccagttg ggacaccgac agtcccgaga cgaggatccg gctcttgagt tcggcgacgt
     5881 cggcggcgtc gacgagctcg ccggccacct cgggcaccgg gtcctgccag atcagccgct
     5941 cggaggggac cagcggcccc aggtagcgct ggaccgggcc catgtcgatg tgcgtgagct
     6001 tgaaccaggc gcgggcgaac gcgtccgcca gccggtccgg gttctccagg aagtgtcggg
     6061 cgatgggccc gtagacaggg tcctcccgca gcgcgaggtc cgtcgtgagc atcctcgggg
     6121 tgtgggtctt cgacgggtca tggggatccg gcacagtgcc tcgcccttgg ccgtcgccgg
     6181 gaatccactg ccacaatccg gcggggctga gctcgacgtc ccactggtag gcgaacaggg
     6241 tcttgagaaa gctgttgtcc cacgtggtgg gggtgggagt ccagatgccc tccagcccgc
     6301 tggagatcgt gtcggggccc ttgccggtgc cgtagctgtt ctgccagccc aatccctgct
     6361 cctcgatcgg ggcgccttcg ggctcggcgg cgaggtggcg gcccgggtcg gccacgccat
     6421 gcgtcttgcc gaacgtgtgc ccgcccacga tcagcgcgac ggtctcctcg tcgttcatcg
     6481 ccatgcgccc gaacgtggtc cggatggcgc gggcagccgc tacggggtcc ggcagggtgc
     6541 ccgggccctg cgggtcgacg tagatgaggc ccatctcatc ggcggccagc ggttcctcca
     6601 ggtcccggac accggtgtgc cgctcgtcgc cgagccaggt acgttccgga ccccagtaga
     6661 cgtcctcgtc ggactcccag acatcctccc ggccgcctgc gaagccgaag gtgtcgaagc
     6721 ccatcgtctc cagcgcgcgg ttgcccgcga acaccatcag gtcggcccag gagatcttgc
     6781 ggccgtactt ccgcttcacc ggccacagca atcggcgcgc cttgtcgagg ttgcggttgt
     6841 ccggccagct gttggtgggt gcgaagcgct gcatacccct gctcgcaccg ccacggccgt
     6901 cgccgacgcg gtacgtgccg gcgcagtgcc acgccatccg gagcacgagc ggtccgtagt
     6961 ggccgaagtc ggcgggccac cagtcctgcg aggtcgtcag cacctcgtcg atgtcgtgtg
     7021 ccagagcgtc caggtcgatg gtccggaatt cggcggcgta gtcgaattcc tcgcccatgg
     7081 cgcccgttgc gggctgatgc ttggtcagca ccttcaggtt gagccggttg ggccaccagt
     7141 cacgattgtc cccaccggca gccgggtgac cacggcggtc ggccgcacgg cgtgtgccca
     7201 ggatctggtc gtacgtcctg ctgatgacga cggcgtcagg gttctccggc acggcagctc
     7261 cttccggggc ctcgaagccc ctcagatatt tccaggaggt ggtcgccggt gtgcacgaaa
     7321 cggccggtca ccctctacta cggcgtcgcg ccgacgcatg gccggcacgg ccacatgttt
     7381 cagtgccccc gggccaccca atccagcagg tgtggtgcct cggcgccgat ccaggtcgcc
     7441 tcgccgtgcc cggtgcgcac ctcgacctcc ggcggcaggg tgagcagccg gtcccggacc
     7501 gactcgatga tcgtgtcgaa cgaggagaac gaccgtccgg tagcgccggg gccgccgaag
     7561 aagagggtgt ccccgctgaa gagtgtgccc agctcagggg cgtacagaca ggtcgatccc
     7621 ggcgagtgcc ccggagtgtg cagcgcgcgc agctcgactc cgcctgcgcg tagcacgtcg
     7681 ccgtcggcca tagcccggtc gggtgtgcgc tcctgccagg tcatctccca cagcggcgcc
     7741 tcggccgggt gcagcacgat cggcgcgccg tatcgatcgg ccagcgcggg tgcctggttg
     7801 acgtggtcgt cgtgtgcgtg ggtgcacacc accgcgacca cccgccggtc ccccaccgcc
     7861 gccgcgatcg cgtcggcgtc gtgcgcagcg tcgatcacca gcacttcgac gtcgtcaccg
     7921 acgatccaga cgttgttgct cacctcccag accccgccgt ccagcgagaa cgtcccggag
     7981 gtcacgacgc gttggaccgc cggctcgtgg ctgtggacct cgcgccggct cacagcacca
     8041 ccaccgagcg cagtacctcg cccggtcccg ggtccggaac cgtgatcgtc tccaccgaca
     8101 ccggcttgcc cctgctgtgc gaaaccactc cgcttacctg ctgcgccatt tcccactccc
     8161 ttgtcgatcg ggtccgttcg atccgtcaga aacagctccc gaaccaccgc cgcgacatcc
     8221 gtacgtcatg tcgtccgcct cgttggcacc ttgggtgctg agtgccactt cgacaaggca
     8281 atcgtcgatc gcagagaaag gggcaaggat ttattcggac ttgcagaaaa tctcgccggc
     8341 ccctttcttc gcctaccttg ccggaacgta aatcagcgag tcggatcgat tcgattcggc
     8401 cctggaggac agcgccacga cgggcgtgcg ccgcacgggc gtttcccggc cgagcacccc
     8461 ctcggccacg tgcgccgagg cggcagagcc ggacgaatcg agcccagata ctcgtgcgcg
     8521 tatcacgtga tccgcaacgg ctcagccgcc aacggcgtta atcggggctt gaacggtagc
     8581 cattggcgaa cgaacggcag aggccgggaa gaaatgaacg ggcacccttg tgggtgcgtt
     8641 atcggctgcg tacagtacga gcccaatagc gcccctggcc actcgcctcg accgcccggc
     8701 accaccggat ctcggccgac ggctaaaagc tctccgaatc cggtgccatc accgggtgcg
     8761 cgccagggac gggcctgagc ctgcccatcc ctccgtcatg agatcgaccg agaggagata
     8821 agcaaatggc atcgtgggaa acgcccgaat tcgaagagat cggctgcgga gccgaagtca
     8881 ccatgtatta cttcgccgag ctggacgact agtcggttag tcggtccggc cggtcggacg
     8941 cacagctggt cggtcggtgc ggtgccggat ccacgtgttc ggcgcccccg gcctccagca
     9001 ccgggcgaca ccccgtcgaa gtcgacctcg aagtcgaccg cgaagtcgac caggagcgga
     9061 agaaggacca ggatgtggct gcgggtgctg ggttcggcgg cgggcggcgg atttccgcag
     9121 tggaattgcg gctgcccggg atgccgcgcg gtacgagacg gttctcggcc ctgccggccc
     9181 cgcacccagt cctccatcgc cgtgagcccc gatcaccagc ggtggttcct cctcaatgcc
     9241 tcccccgaca tccacgctca cgtcgagtcc ctgtcggccc cgcagtcgag caagggccgg
     9301 acgacgccgc tggcagcggt actgctcacc gacgccgagc tcgatcacac actcggcctg
     9361 ctgctcctgc gcgagggccg ggccctcgaa gtgcacgcca ctcaagcggc gcacgaggcg
     9421 ctgtacgacg ggacatcgct gctccgaacc ttggagcgct actgcgcggt cgagtggcga
     9481 ccggtcgttc caggagccga cgtgtccctg ggggacggcc tcagctaccg ggccttcgac
     9541 gtacccacga ccaagcaggc gcgcttcggg cccaccagcg ggcagggccg ggtggtgggt
     9601 taccgcctga cggatgagcg cagcggccgg tcagcggtgt acctcccggg gctgcagcag
     9661 ctcactccgg cgatacgcga gcagttggac gactgcgcgt gtctgttcgt cgacggaacc
     9721 tgttggcacg acgacgagct gatccggctc ggcttagccg gcaagacggc acgagagatg
     9781 ggccacctac cgatcgacgg tccggacggc agcctcgaac agctttcacc tcttcccgtc
     9841 gagcgcaaga tctacgtgca catcaacaac accaatccca ttctcctgga ggacgcgcct
     9901 gaacgacgtc tggtcgagaa gcagggcatg gaagtggcca tggacgggct agagatacgg
     9961 gtgtagagct gcgatgacgt caaccgaggt cgaaacagaa gtgacccacg acctggtgga
    10021 ggcgcttgat ctggtggagg cgctgcgcgc ccagtcgcgc cgataccacc atcagcaccc
    10081 cttccacctc aggatgaacg agggtcggct gagccggcac cagatccagg gctgggtggc
    10141 gaaccgctat tactaccagg tgaacatccc ccgcaaggat gccgcgatcc tcgccaactg
    10201 ccccgacatc gacgtgcgcc gtcggtggat ccgtcgtatc cacgaccacg acggcacccg
    10261 aagcggcgag ggcgggatcg aagcatggct gcgtctgggt gacgcggtgg ggctgacccg
    10321 cgaagaagtg caggacgaac gccatgtcgc ccccggggtc agggccgccg tcgacgacta
    10381 cgtcaccttc gcccgcaccc ggccctggcc cgaggcggtg gcgtcctcgt tgaccgaact
    10441 cttcgccccg gacctgatgg cccagcgcat cgtcgcgttg gagcgcttct acccctggat
    10501 cgaccgggac ggcctggcct acttccgaac tcggctggat caggcgcccc gcgacgccga
    10561 acacgctctg gaggtcgtga ccgcgcactg tcgatcggct gaggcacagc aacgcgcagt
    10621 ggcagcactc acgttcaagt gcgatctgct ctggaggatg ctcgatgcca tcggtgaaac
    10681 ctacccggag tgaccggcgc gcggccgacc gacgccgtac cgcaacgacg tcgtcgagcc
    10741 gtccgaaact gacgtcccac gttcgcctgc ggttcgacgc ggcgcgggaa caacacgtcc
    10801 tgctggggcc ggagtcggtc atggtcctca acccgacggg cgcggacatc ctcagcctgt
    10861 gcgacggtcg ccacacggtg gcggagatcg tggcgacact ccaggaccgg tatgacgggg
    10921 tggtcgacga cgaggtgcgg cgcttcctcg accggctggc caccaagcgg tgcctggaga
    10981 tgagcgatgg atagcccctt cggtttattg gccgaactca cctaccgctg cccgttgtcg
    11041 tgtgcgtact gctccaaccc ggtcaacctc ggcgactacc acgacgaact gaccacggcg
    11101 cagtggcagc gcgtgctgat cgaggcccag gagctgggcg tgctgcaact ccacctgtcg
    11161 ggcggagagc cgctgctccg tcgtgacctg gtcgacctgg tgaccagcgc gcggtacctg
    11221 ggcctgtaca ccaacctcat caccagtgcc gtcggcctgt cccggcgccg cgcggagcag
    11281 ctcagagcgg ccggtctcga tcacgtgcag atcagcatcc aggccgacga ggcgaccctg
    11341 tccgacggga tcgccggtac tccgtcctac gcgggcaaga tcgaggcggc tcgcctggtg
    11401 aaggagctgg gttggcccct caccttgaac gtggttctgc atcgccggaa catggaccgg
    11461 gtcgccgccg tgctggatct ggccgaggaa ctggcggccg accacctcga attggccaac
    11521 acccagtact acggctgggc gtggcgcaac cgtgatgcgc tgttgcccag cagggcgcag
    11581 ctcgatcgag cggaagcagt ggtgcgtgcc gcgcggaagc ggctcaaggg acggatggac
    11641 atcgtctatg tccttcccga ctattacagc cagtacccca agccctgcat gggtgggtgg
    11701 gggaagcggc agctgactgt caccccgaac ggcgacgtcc tgccctgccc ggccgcgcac
    11761 gcgctgcctt tgccgcgcgc gagcgtgcac gaggactccc tggcacggat atgggcccga
    11821 tctccgctgt tccagagttt ccgcggtacg gactggatgc cggagccgtg ccgaagctgc
    11881 gagcgccgcg agatcgactt cggcggatgc cgctgccagg ccttccagct caccggcgac
    11941 gcgtcgcgca ccgatccggt ctgccacctc tcccccgacc acgggatcgt ggccgagagg
    12001 gtgcgggccg ccaacgagga gaacaggctc ggcgacagcg tactcgtccc ccggccacac
    12061 cgcggccgcc cgggccggcg gtagtgcggc ggttcccggt gccagtggct ccacgaccgc
    12121 catcgttgca acccaccgcg gctaccgacc acagcgggtc cagacgcagg aggccctgac
    12181 acatgcactc gatctggttg ctcctcggcg ccggcttcgt cctcggcttg gacaatttcc
    12241 gggtggcgat cgtgctcggc acggttccgt tcagcctccg ccgtgcattc caagtcgctc
    12301 tgatattcgg cgtgtgggac ggggtgatgc cgctgatggg cctgctgctc ggtcgttatg
    12361 ccggccaggc gatcggcccg atctccacca ttgcggcctc gatcgcgctg ggcggttacg
    12421 gcctgtacct gctcatccag gccttgcgga acccggagtc ccccgacgag atcgacaacc
    12481 cgtgggtgac gctcttcggc atcccgctct ccttgagcct ggacaacctg gtcggcggcg
    12541 cgagtctcgg actgctcgga ttcgccccct gggtcacggc catcgtgttc ggtgccgcta
    12601 ccgcggtgat gacgttgatc gggctgcaca tggggaggtt cgccgcacac ctcatacgta
    12661 tccgctccga cctgctcagc ggtgtcgcgc tcatgctcgc ggccgtcacc ctgcccctct
    12721 tgcccaactg acgcgacggc gccgcgcccc acagcacttg gcggaagagc gcctcgatgt
    12781 cacctgtgga cacggccccg ggcggtccag ccccgattca caggcccagt gcccgacgca
    12841 cggctgacgc atgccgccgt gagaccggca ccatgtcgtc cttccggttg tccatcacca
    12901 ggaacagttc ccccttgaac ccgcgctcga tctcccggac ccggctgagg ttgaccacgt
    12961 accggcggtg gacccgcagg aagccggcat cggtcagctc ctcgtcaagc ttgtccaagc
    13021 cctgggaggc ggaacgaaac cgcccctggt cggtgaagag ccacacgttg ttgccgtccg
    13081 actcggcgaa gcacacctcc ggcagccgta gcagcaccgt ccggtcctcg cgcaccgcca
    13141 ccagccggcg cggcttcatc cacggatggc cgggccccgt cgctgggagg gtttgcgccc
    13201 cctcggaggc gccgccgagc cagatcaggt gcccgacgag gccaccgcgg aggaacacgg
    13261 gccggatcgt gatcggcgtc ggttcgtcgc ggaggtgggc gtagatctgt gtcgaaccga
    13321 cccagtccgg ggtgtgggcg gccgtcttac tggcgtaacg cacgatgcgg gtgaggtcag
    13381 gcagcccgga gtcccatcgc tggtccgggt cgactgccga gacgtagggg cgaacgccca
    13441 tcagaaggct cgccggctcg tcagcgatca ccaccttgcc cgccgtgtcc accgcggcta
    13501 ctccggtgcc ggagagggcc ttggcctggg tgaatgcggc ggccaactcc gcgccgctgt
    13561 cacgagcgcg ccggttcagc gtcccctgcg ccgtggtggc cgcgttaccc agccaccggg
    13621 tcgcggcctc cggaagttgg gtgcgccagc aggagatgtt catcaccgcg atcggctcgc
    13681 cggtcaccac gtggcgcacc gccacgccgg cacagaccca gtcgtggaag gccctgcacc
    13741 agtgctccgc acccctgatc agtacggggc cgcgcgcctc aagagccgtg cccatcccgt
    13801 tcgtgccgac tgcgcactcg ctccagcagc cccaaggcgc cagattggcg tcggaggctc
    13861 tgacgagagt ggcacggtca ccccaggagg ccaggatccg gccggcggcg tcggcgacgg
    13921 tgacgatgcc gttgagcccg tccacctcgt gcgccacgga cgcggcgaga ccacccagtt
    13981 cagtgaagat cacgtcatgc tcaagcgtat ggtcgacgct tccgagagct gagggagctt
    14041 ccgtgagatg tgggtcgacc cggtactgct cccggcagcg atgccaggag atcgccacct
    14101 cgggccggac accgcggaca tcgtcctcgc cctgcatgaa cctttcccat gcggccagca
    14161 ccgtgcctct gccgacggtt acggacgtgg gctcgggccg acgcagatcc ttcacgagga
    14221 gaaccgccct cggtccatcc ataaccaacg ccgcgtcgcc tgcgctcggt acacgccagt
    14281 ggccggaccg ctggtcacag ccaacagcaa acgggacatg ctgtcacgca ctgtgattga
    14341 gctcaacaac atcaccacca ccctgtccgt ctctacagcc gaaagacatc tcggtcaggt
    14401 cccggggacc cggtaggcga gcagcacgtt cgaccggtcg aaaaatgacc gatcccactc
    14461 acggcaccct cgaacgtgtt tcacgccagg gtcacccagg ccgggtgata gccagtcaag
    14521 tgcgcaaact gaccctgccc catgggtagt tgcggtgcgg tgtgtgttgt gtagtcagaa
    14581 tacggtcgac aggcgaacag ggtcaacggt cgcagccggg cgcactccac ggcgttgcgg
    14641 acgctgcggg cactggcgga acctcggctg cgtccgctgt ggccacgcga tcggcacttc
    14701 atgcaaaatg cctgctcagc gcgctgagca ggcattgcca aaaagtgtcc gaggggggac
    14761 ttgaaccccc acgcccgata aagggcacta gcacctcaag ctagcgcgtc tgccattccg
    14821 ccacccggac caggtgtctg ccgcctggac ggaggtgctc cccgcggcga catggacaac
    14881 aataccaagc tttcggagtg gctttcacct gcgtttcctc gccccgacgc gtgcctggcg
    14941 gagcacggag agcacgacca gaactcacca tgagtgaccg tacgatcacg agacgagcgg
    15001 cgccgatcac gcggcgtcgc ccgcctcgcc gtacggcatc acgtcacggc atcacgtcac
    15061 ggcatcaagt ggtactccgg gaagttcccc ggcagccgtt cccccgccgg cccccgcgtg
    15121 accgcgcgca ccagcagttc gccgcccacg aacgcgccgc gccaggacgc gccgaagccg
    15181 ccgaacagtt cgtcgcggtc gccgcgtgag cggggctttc cgtggccgac cttgaaggcg
    15241 cggatctgcg gggccaggcg gctgtacgtg gcctcgtcgt ccgtggacag ggtcgcgacc
    15301 agcgcgccgt tcgacgcgtt catggcagcg agcagttccg cctccgtgtc gaccaggacg
    15361 atggtgtcga ccggaccgaa gggttccgcg tggtggagcg gggaggacgg gggcgggttg
    15421 aggatcgtga ccggctggac gtacgccgag gtgtcctggc ccggcaggaa gcgggcgtcg
    15481 gcgagcttgc cgcggtgcag ggggacggcg ccccggtcga tcgcctcggc gacctggtcg
    15541 tgcagctcct tcgccttggc cgcgttgatc accggcccga agtccagccg tgggtacgga
    15601 tcgcccggct gctcgacggc cagggggtgg ccgacgcgga gggtgtcgac cgcggggagg
    15661 tacgccgtca ggaactcgtc gaacagctca cgctggacga cgaagcgcgg gtatgccgtg
    15721 cagcgctgct tgccgtagtc gaagagcttc gggatgacgg tcgtcagcgc gtcccagtcc
    15781 gtgtggttcc agatgcccca ggtgttcagg ccctcctgtt ccagtacgtg ccgcttgccg
    15841 aggtcggcga cggccgtggc caccgcggcg cccgtgtcac ggccgccgac gaaggagacg
    15901 cagccgatct cgggcgcgcg cacgagcgcc tccgacagct cgcctccgct gccgctgacg
    15961 agggtgacgg gtattccctc gcgggcggcg agtgcgcagg ccagagtgag gcacgcgacg
    16021 ccgccgtcgg tcggggtctt ggcgatgacc gcgttgcctg ccaatgcctg taccagcatt
    16081 gcgtgaacga gcacgctcat cgggtagttc cagctcgcga tgttggacac gggaccgtcc
    16141 agcggggtcc ggtccgcgac catgggttcg attccgtcga cgtaccagcg cacgccgtcg
    16201 atggcacggt cgacgtcggc ctgcgcgagg cgccagggct tgccgatctc ccagacgagg
    16261 aggagggcga gcagttcgcg gtgctgggtg agggcgtcga gggtggccgc gacgcgggcc
    16321 cggcgttctt cgaggggtac gtgccgccag gcgcggtggt ggtcgaggca ggcgcggacg
    16381 gcctgctggg ccgtggcgcc gtccagtcgc ggcggcccgg cgatggggct cccgtcgacg
    16441 gggctggtgg cgggcagtgc ccggccgtcc gcctgccagg cggcgcccca gagattgagg
    16501 acacggtcgt cccggaaggc ctcgggcgcc acggccagac agcgctgcca ggcgtcggcc
    16561 cacgaggtgt gggacttgag ggtgagggtg ggggtgggag tgggggtggg ggtgggagtg
    16621 gatgtcggtg ttggtgtcgg tgccattcgg ttgctccgct ctcggtgcac agtcgggggc
    16681 ggtcgtcgta cgggacgtca gcaggactcg gcggcacccg caagtagcca ggcgtcaacc
    16741 atgagtgaca tcacgtgccg cttcgagcct tgccagtacc agtcgggccg tctcggtcgg
    16801 ggtgtcgccc acctggacgc cggccgcttc cagggcttcc tttttcgccc gggccgtgcc
    16861 ggacgagccg gacacgatgg cgcccgcgtg gcccatcgtc ttgccctcgg gggcggtgaa
    16921 gccggcgatg tagccgatga cgggtttggt gacgtggtcg cggacgtacg cggccgcgcg
    16981 ttcctccgcg tcgccgccga tctccccgat gaggacgatg agttcggtgt cggggtcgtc
    17041 ctcgaaggcg gccaggcagt cgatgtgggt ggtgcccacg acggggtcgc caccgatgcc
    17101 gacgcaggtc gagaaaccga tgtcccgcag ctcgtacatg agctggtagg tgagtgtgcc
    17161 ggacttggag accagtccta cgcggccgga cttggtgatg tcggcgggga tgatgcccgc
    17221 gttggactgg cccggggtga tcagtccggg gcagttgggg ccgacgatcc gggtgccctt
    17281 cgcccgcgcg tacgtggtga aggcgaccga gtcgtggacc gggatgccct ccgtaatgac
    17341 gacggccaga ccgatgccgg cgtcggcggc ctccacgact gccgccttgg cgaaggcggg
    17401 cgggacgaag acgacgctga cgtcggcgcc ggtcgcctcg acgccttcgc gtaccgagcc
    17461 gaagacgggg acggcacggt cgtcgaagtc gacgctgcgg cccgccttgc gcgggttgac
    17521 gccgccgacg acgtcggtgc ccgcggcgag catgcgccgg gtgtgcttca tgccctcgcc
    17581 gccggtcatg ccctggacga gtaccttgct ctctttggtg aggtagatgg ccatgtcccg
    17641 ctccttcagc ctgcgttggc gagttgtgcg gcgcggcgcg cggcgccgtc catggtggtg
    17701 gcctgctgga ccagggggtg cgcgcggtcg tcgaggatcg tgcggccgcg tacggcgttg
    17761 ttgccgtcga ggcggaccac cagcggtttg gtcaactgga cggtgtccag cgcccgtacg
    17821 atgccgtcgg cgaccgcgtc gcaggcggtg atgccgccga agacattgac gaagaccgac
    17881 ttgacggccg ggtcggagag gatgacggag agcccgtcgg ccatgatctg ggcggaggcg
    17941 ccaccgccga tgtcgaggaa gttggcgggg cgggcgccgc agccggcgac cacgtcgagg
    18001 gtcgacatga cgaggcccgc gccgttgccg atgatgccga cctcgccgtc cagcttcacg
    18061 tagttgagac ccttggccgc ggcggccgcc tccagcgggt cgtcgtgcgc cacctcctgg
    18121 ccgccccacc gtgcctgccg gaagcgggcg ttgtcgtcga gggtgacctt gccgtcgagg
    18181 gcgaggatct gtccctgggc cgtacggacg agggggttga cctcgacaag gacggcgtcc
    18241 tcgcggacga gtacgcccca gagtctgacc agggtgtcga cggtctgcgg gggcaggcct
    18301 gcggcctcgg cgatctcggt ggccttcgcc gaggtgacgc ccacggccgg gtcgatgtgg
    18361 atgcgcgcca ccgcgtccgg ccgggtggcg gcgacctcct cgatgtccat gccgccctgg
    18421 gccgaggcga tcgcgaggaa ccggccggcc gcgcggtcga gtacgtaact gacgtagaac
    18481 tcgctgtcga tgtcgaccgg ttgggccagc atcaccttgc ggaccgtgtg gcccttgatg
    18541 tccatgccga ggatctgacg tgcggtcagt tcggtggcgg ccgggtcggc ggcgagtttc
    18601 acgccgcccg ccttgccccg gccgccggtc ttgacctgcg ccttgacgac gacgcggccg
    18661 ccgagcccgc gggcgatctc gcgcgtctcc ttggacgagt cggtgatctc cgccctcggc
    18721 accggaatgc cgtgttcttc gaagagttcc cttgcctggt actcgaacag gtccatctgg
    18781 gctccctctg atcccttggg tccctctgcg gatccctttg atctctctga aaagtgccgc
    18841 acgccccctg gacaccaccc accggatgcg ggataacaag cttcatacag tattcgtcga
    18901 ctgtatgcaa tgtaccgcga cggcatccac gaacagtgcg cgcaccacgt atgagctgca
    18961 cttccgcgcg cccaatcccc tacgaaggga caggacttcg ccatgcccga cgacaacagc
    19021 cagaacctca tctccggtgg gcacctggtc gccaaggcac tcaaagcgga gggtgtggag
    19081 gtcatctaca cgctctgcgg cggccacatc atcgacatct acgacggctg cgtcgacgaa
    19141 ggcatagagg tggtcgacgt acgccacgag caggtcgccg cccacgccgc cgacggatac
    19201 gcgcgcatca ccggcaagcc cggctgcgcc gtcgtcaccg cgggtcccgg cacgaccgac
    19261 gccgtgacgg gtgtcgccaa cgccttccgc gcggagtccc cgatgctgct gatcggcggc
    19321 caaggggccc atacgcagca caagatgggg tccctccagg acctgccgca cgtcgacatg
    19381 atgacgccga tcaccaagtt cgccgcgacc gtgccggaca ccgcgcgggc cgccgacatg
    19441 gtgtccatgg cgttccgcga gtgctaccac ggggcgccgg gaccgtcctt cctggaaatt
    19501 ccgcgcgatg tccttgacgc caaggtgccg gtggacaagg cacggatccc cgccgccggc
    19561 cactaccggg cctccacccg ctccgccggt gaccccgagg ccgtcgagaa gctcgccgac
    19621 ctgctcgtgc acgccgagaa gccggcgatc ctgctgggca gccaggtgtg gacgacccgc
    19681 ggcaccgagt ccgccatcga cctcgtacgc accctcaaca ttcccgccta catgaacggc
    19741 gccggccgcg gcacgctgcc gcccggggac ccgcaccact tccagctctc gcgccggtac
    19801 gccttctcca acgccgacgt catcgtcatc gtcggtacgc ccttcgactt ccgcatgggc
    19861 tacggcaagc ggttgtcgcc cgacgcgacc gtggtgcaga tcgacctcga ctaccggacc
    19921 gtcggcaaga accgggacat cgacctcggc atcgtcgggg acgcggggct cgtcctgaag
    19981 tcggtcaccg aggccgcctc cggacgcctc aacgggggtg cgtcgaagcg caaggagtgg
    20041 ctcgacgaac tgcgcgccgc cgagcagacc gccatcgaga agcggctgcc gagcctgaag
    20101 tcggacgcct caccgatcca cccgtaccgg ctggtcagcg agatcaacga cttcctgacc
    20161 gaggactcca tctacatcgg tgacggcggc gacatcgtca ccttctccgg gcaggtggtg
    20221 cagcccaagt cacccgggca ctggatggac ccgggaccgc tcggcacgct cggcgtcggc
    20281 gtccccttcg tgctcgccgc caagaaggca cggcccgaca aggaggtcgt cgccctcttc
    20341 ggggacggcg ccttctcact gaccggctgg gacttcgaga ccctcgtccg ttacgacctc
    20401 cccttcgtcg gcatcgtcgg caacaactcc tccatgaacc agatccgcta cggccaggcc
    20461 gccaagtacg gcccggagcg cgagcgggtc ggcaacaccc tcggcgacgt ccactacgac
    20521 aagttcgcgc agatgctggg cggttacggc gaggaggtcc gcgaccccgc cgacatcggc
    20581 ccggcgctcc agcgcgcccg tgagtcgggc aagccgtcgc tcatcaacgt ctgggtcgac
    20641 ccggacgcgt acgcccccgg aaccatgaac cagacgatgt acaagtgagg tgtccccgat
    20701 gacagcagtg acgaccgcca cggcatcgac gcccgccacc gcgaccaaag ccctcgaagg
    20761 cgtccgcgtc ctcgacatga cgcacgtcca gtccggtccc tccgccaccc agctgctcgc
    20821 ctggctcggc gcggacgtcg tcaaactgga ggcgccgacc ggagacatca cgcgcaagca
    20881 gctgcgcgac ctcccggacg tcgactccct ctacttcacg atgctcaact gcaacaagcg
    20941 gagcatcacc ctcaacacca agaccgagcg cggcaaggag atcctcaccg agctgatccg
    21001 gcgctccgac gtcatggtcg agaacttcgg accgggcgcg gtcgaccgca tgggcttcac
    21061 ctgggaccgc atccaggaga tcaatccgcg gatcgtctat gcctccatca aggggttcgg
    21121 ggacggcccg tacaccaaat tcaaggcgta cgaggtcgtc gctcaggcca tgggcgggtc
    21181 gatgtcgacc accggcttcg aggacggacc gccgctggcg acgggggccc agatcggtga
    21241 ctcgggcacg ggcatccacg cggtggccgc gatcctcgcc gccctgttcc agcgggagaa
    21301 cacggggcgc gggcagcgcg tgaacgtggc gatgcagcac gcggtgctga atctgtgccg
    21361 ggtgaaactg cgggaccagc agcgcctcgg gcatgggccg ctggctgaat atcccaacga
    21421 cgacttcggc acggaagttc ccaggtccgg aaacgcgtcc gggggcggcc agcccggctg
    21481 ggccgtcaag tgcgcgccgg gcggccccaa cgactacgtg tacgtcatcg tgcagcccgt
    21541 cggctggaag ccgctcaccg agctgatcgg caggcccgag ctggcggccg accccgaatg
    21601 ggcgaccccg gaggcgcgcc tgccgcagct caccaagatg ttccagctga tcgaggagtg
    21661 gtcggccacg ctccccaagt ggcaggtgct ggagaagctc aacgcccaca acatcccgtg
    21721 cggcccgatc ctctccacca aggagatcat cgaggacgag tcgctggtcg ccaacgagat
    21781 ggtcgtgacg gtgccgcacc ccgagcgggg cgacttcgtg accgtgggca gcccgctgaa
    21841 gctctccgac tcccccgtgg acgtgaccag ttcgccgctg ctcggcgagc acaacgaaga
    21901 ggtgtacgtc ggcgagctcg gtctcggcga cgaggagctg cgcctgctca agtcgaacgg
    21961 agtgatctga ttgatggccg aagaccgggt gctgagggtc cgcgcactcc tcgactccgt
    22021 gcgggccgag gggcggacgg cgctgaccgc gcccgagggc aaggtggtcg ccgacgcgta
    22081 cgcg
//
