LOCUS       NZ_AP023440            10789 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces aurantiacus strain JCM 4677 chromosome.
ACCESSION   NZ_AP023440
VERSION     NZ_AP023440.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces aurantiacus
  ORGANISM  Streptomyces aurantiacus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 9428393)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye
            Genome Coverage                   :: 62X
            Sequencing Technology :: Illumina HiSeq X Ten; PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014701115.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 05:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,211
            CDSs (total)                      :: 8,122
            Genes (coding)                    :: 7,928
            CDSs (with protein)               :: 7,928
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 69 of 194
            Pseudo Genes (incomplete)         :: 161 of 194
            Pseudo Genes (internal stop)      :: 17 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:48:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1117858
            Orig. end                         :: 1128647
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023440.1.
            Genome sequencing and analysis were conducted under the Global
            Catalogue of Microorganisms (GCM) 10K type strain sequencing
            project (http://gctype.wdcm.org). The genomic data are also
            available at http://gctype.wdcm.org/sequencing/GCM10017557
            Annotated by DFAST v.1.2.7 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10789
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="6"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10789
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10789
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5789](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5789)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(596..1567)
                     /locus_tag="IGS71_RS04780"
                     /old_locus_tag="GCM10017557_09830"
     CDS             complement(596..1567)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093175.1"
                     /locus_tag="IGS71_RS04780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_09830"
                     /product="hypothetical protein"
                     /protein_id="WP_190849489.1"
                     /transl_table=11
                     /translation="MTVTGASHPVARESRSAAASARRLRAFFAERAPARIYVVYAVLWV
                     LALQGTLALLSPERDWKLGGSLVAEIATIYLALLFIRVVDEQKDLEYDRLHSPDRPLPR
                     GAIDVRELRIAMALIVVAELALTGWRSPLLVGWLLLNLSYICLLVGLERWSATVRDRIF
                     VNLLVSYPAQAMLSVHIWLSFREATGAGPGSHAVLGALLCAGVFMHYEFARKTGATVVP
                     GATLYSNVVGLRGSVALTVGCALTGMALVLGVIRPWELHGTVTLAGWLPLTALGFLWGG
                     AERFTSSNTPTWPPGPAMGYLAWLYLGVFVAALAVVRPGIGG"
     gene            complement(1564..4290)
                     /locus_tag="IGS71_RS04785"
                     /old_locus_tag="GCM10017557_09840"
     CDS             complement(1564..4290)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PPDK_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712904.1"
                     /locus_tag="IGS71_RS04785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_09840"
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="WP_190849490.1"
                     /sec_met_domain="PPDK_N (E-value: 2.3e-55, bitscore: 187.0,
                     seeds: 105, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNARRALRIPTFSSHRRKGPVMRGRALLNEAEVGPKFSRQARMAT
                     EGFPVPRFFCVSAATFTEVLRPVRAELDALLAGLDTADAETLEKRASALRETVLAASVP
                     AAVARQLYDAFDEVFGPDAVVSVRSSVVGTGADVGEDSAHDAFAGISDSFLYVTREELL
                     DRVRQCWASGFGTQALLYRAARGGTQHGFAVAVGVQRMEFGRRSFVLFTCDPLTGARDR
                     VLAAGLGIGEGVVQEKVPVDHYFVSRRDNAVRSVLAHKTQLMEENPGRLADGPRTVPAP
                     EELWDAPALTDEQIQQVVMVGDRIEELFGCPQDIEGTFTADGRLNILQARPVVLELDRQ
                     RLWSNANITESYPDVTTALTYSFAQRFYREDFHDFYRLLGVPRKVLDRHEGELRGMIGL
                     LRGRVYYSLSVWYALHGLSRTFPLWRRSFERMMGMSPSVTHVRPTPPLTDPALLPATAA
                     ATARLAWRLVTHGAAVKDFETWWERILTAHREAAKTADPLQLAQLMRSLWTEVGDRWGI
                     TLVNDLLLQLHETLAMKMLGRWVPQADAGLHSDLLCGGEENRSVIILMSIVEIAERIRA
                     TPGVLDALAVKPAEEVWDEIDRDAYGSDLADRMRTHNHLHGDRGLQELKLEVPVPRDQP
                     AQLLRIAAEYARGDLTRALLREREAQARRAGERRLAELLAGRPLAGVALRAALAGQRRY
                     INMRENTRYGRSELFGFGRQVYRRLGADLAARGLLEKAEDVMHLTEEEILGTYDGTAVT
                     DDLRGLAAVRRAEFHARGAELPMDFVTMGPVRDALPDPNRDTAASGTLRGLGSSGGIVR
                     GTARVVIDPHDLGDPSEDMILIARETDPGWLFLMLSSRGIVVERGTLLSHTAISGRKFG
                     IPTIVALPHATTRIPDGARVEMDGAAGTVKILEEDEA"
     gene            complement(4299..4874)
                     /locus_tag="IGS71_RS04790"
                     /old_locus_tag="GCM10017557_09850"
     CDS             complement(4299..4874)
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1150: isoprenylcysteine carboxyl methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009999317.1"
                     /locus_tag="IGS71_RS04790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_09850"
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="WP_246596016.1"
                     /transl_table=11
                     /translation="MVRGLLDLSAPWQRVAVALAGLHLMWILLETWTTVRSSTSVEMAT
                     DRGTIYVYATARAATVAAACLPAADDQTYHPWMLALPVVFAAAVGFRLWGIRTLGRFYS
                     HRVRVLGDHQVVQSGPYRWVRHPAYLGMALAHVVFVLFFLNTYSAAALMVLLPTLVVRI
                     LVEERTLLTLPGYSDYAATHRRVIPWIW"
     gene            complement(5001..5789)
                     /locus_tag="IGS71_RS04795"
                     /old_locus_tag="GCM10017557_09860"
     CDS             complement(5001..5789)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093172.1"
                     /locus_tag="IGS71_RS04795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_09860"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_055509470.1"
                     /sec_met_domain="AfsA (E-value: 4e-13, bitscore: 48.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEKFVFVVGDNLACRSNSEDLVPLSQLDLLLEEGLSSDPPLVIV
                     PGQGIEKDERDIVRAKLRRRGLPESMLMDLPLPTLLHRHEVHKHNPENVLIAGLRKVDD
                     QLFRATLRISDRQEMVLDHATGSHVTGMVIQEAVRQMTLAVGERHLLAPLDTARRFVIS
                     SLQTTFHKFLLPLPTQVEYTVEELKRKGPNRLRFRGRCDLLQADVLAASGSMDVMVIDG
                     QRADQIEADHIRVTTRALAELYAEKQGVSEHLTLGQGAAS"
     misc_feature    complement(5715..5717)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6253..6273
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.78"
                     /tool="antismash"
     tRNA            complement(6359..6432)
                     /anticodon=(pos:complement(1124254..1124256),aa:Pro,
                     seq:ggg)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="IGS71_RS04800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="GCM10017557_t00040"
                     /product="tRNA-Pro"
     gene            complement(6359..6432)
                     /locus_tag="IGS71_RS04800"
                     /old_locus_tag="GCM10017557_t00040"
     misc_feature    complement(6465..6483)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.84"
                     /tool="antismash"
     gene            6626..6916
                     /locus_tag="IGS71_RS04805"
     CDS             6626..6916
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IGS71_RS04805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_190849492.1"
                     /transl_table=11
                     /translation="MGALTVSKTRPVPTAAGSSMSLWAATGAYVPSAIVTTVSPASRAW
                     SSASRSSTGWRGSAKHRSALAPAWRAASIRSASDDPPSSSRRTSGCTFAAK"
     gene            complement(7533..8477)
                     /locus_tag="IGS71_RS04815"
                     /old_locus_tag="GCM10017557_09890"
     CDS             complement(7533..8477)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123745.1"
                     /locus_tag="IGS71_RS04815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_09890"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_190849493.1"
                     /transl_table=11
                     /translation="MNKATYRAAALGVVAALSLTATACGGSDDSDDNPLTGGSADSGGG
                     KAIVVGSANFPENQLLAEIYAQALEDKGLKVTRKFDIGAREVFYDQVVKGGIGVIPEYN
                     GALLSVAVDKKSTATSTEEINAELTKKLPKSVGILDSAAAEDKDSVTVTAETADKYNLK
                     TLADLKPVAKDLAIGAGSEFKTRTQGGVGLKTVYGVEFGKFQPLDAGAQSTLLKLLKDD
                     KVQAANLYTTDPAIVEDKLVVLEDPKNLFSSQNVTPLIYKSAVNDKAKSALNDLSAKLT
                     TEDLLDMMKKLVNDKEDASDVAKEWLTSAGLTS"
     gene            complement(8593..9282)
                     /locus_tag="IGS71_RS04820"
                     /old_locus_tag="GCM10017557_09900"
     CDS             complement(8593..9282)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005474951.1"
                     /locus_tag="IGS71_RS04820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_09900"
                     /product="ABC transporter permease"
                     /protein_id="WP_055509464.1"
                     /transl_table=11
                     /translation="MNDLLNQIRLVGDWLTSSQQWHGDDGIPHRLAEHLTYSGLSLLFA
                     TLIGLAFGLLVGHTGRGAFAVASVANLARAIPTFGLVVLVVTLAGLSTTPVLVALVALA
                     VPPILINTFEGVRGVDPDARDAAKGMGMTGWEVLLKVEVPMALPLILLGLRVAAIQVVA
                     TATVAAYPGLGGLGRFIVDGLSRNDYELVIGGSAVVVALALVVQAVFTALRRAIVSPGL
                     RPAATKS"
     gene            complement(9279..9944)
                     /locus_tag="IGS71_RS04825"
                     /old_locus_tag="GCM10017557_09910"
     CDS             complement(9279..9944)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139255.1"
                     /locus_tag="IGS71_RS04825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017557_09910"
                     /product="ABC transporter permease subunit"
                     /protein_id="WP_055509462.1"
                     /transl_table=11
                     /translation="MRDGEPLIRWVWIGDHVGELAHYTGVHLRLGLLPVLFGLIISVPL
                     GILCHRWRWIYPPTLTAANVLYSVPSLALFMIFVRYTGLTERTVMIPLTLYTLSVLIPN
                     VVDGLASVPDPVRQAATAMGFSTVRRVIQVELPIAVPVVVAGVRVAAVSSISLVAVGQL
                     IGQGGLGYYIIRGLQLDFPTPIITATVLIMLLALVTDALLVLAQRLLTPWARGKGAKA"
     misc_feature    10474..10488
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    complement(10695..10709)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.19"
                     /tool="antismash"
ORIGIN
        1 aagcccaact cgcgccgcag acggtcggcc ttccagcgtg atccggtcgt gccgatcagc
       61 cgccccgccc cgcgcagccg cgcgaactgc cccgcgatcg acccgacccc gcccgccgct
      121 ccggtcacca ggacggtgtc accggtgcgc accgtgcccc cttgggagac agccagccag
      181 ccggcgaacc cctgggacag agaggccgcc gggtcgggcc agcggccggc gtcgacccgc
      241 cgtaccgagt tcgccgggag caccgcgtac tcctgccagc cggagtcgtg ccggatcagc
      301 tcgccggggc acagccccgt ccccggggcc tccatcacct cgccgagggc tgtgccgcgc
      361 agagcccggc ctggctggta ggggcgcatg ggcaggacgt ccacgtcggc catcagggtg
      421 cgggtggccg cgccgatgcg cagcaatacg ttgcgtacca gcacctcgcc cgtcgccgga
      481 cggcgcagcg ggacccgcgc cgttgcgaag tgctcgggtc ccagctgcgc gtccggacgc
      541 gcagccagcc tgacctcgcg gtggacggcg ggcggcgcga ccctgtgcac cggcgtcatc
      601 ccccgatccc cggccggacc acggccagcg ccgcgacgaa caccccgaga tacagccagg
      661 ccaaatagcc catcgccggt ccgggaggcc aggtcggcgt gttcgatgac gtgaaccgct
      721 ccgccccgcc ccacaggaag cccagcgcgg tcagcggcag ccagcctgcc agcgtgaccg
      781 tcccgtgcag ttcccagggg cggatcacgc cgagcaccag ggccatcccg gtcagcgcgc
      841 agcccacggt cagcgcgact gaaccgcgca gcccgacgac gttcgagtac agcgtggcgc
      901 cgggcaccac cgtggcaccg gtcttgcgcg cgaactcgta gtgcatgaag acacctgcgc
      961 acagcaaggc acccagcacc gcgtgcgatc ccggcccagc gccggtcgct tcccggaatg
     1021 acaaccagat gtgcacgctg agcattgcct gcgccggata gctcaccagc aggttgacga
     1081 agatgcggtc ccgaaccgtc gccgaccagc gttccagccc caccaggagg cagatgtaac
     1141 tcaggttcag cagcagccag ccgaccagca gcggcgagcg ccagccggtg agcgcgagtt
     1201 ccgcgacgac gatcagcgcc atcgcgatcc gcagctcccg tacgtcgatc gcgccgcgtg
     1261 gcagtggccg gtcagggctg tgcaggcggt cgtactccag gtccttctgc tcgtcgacga
     1321 cccggatgaa cagcagcgca agatagatgg tggcgatctc ggcgacgagg gagccgccga
     1381 gcttccagtc ccgctccggc gagagcagcg cgagggtgcc ctgaagggcg aggacccaca
     1441 ggaccgcgta gacgacatag atgcgggcgg gagcccgttc ggcgaagaag gcacgcagac
     1501 ggcgggcgct ggcggcggcg gaacgggact cgcgagccac cgggtgggaa gcgcctgtga
     1561 cggtcatgcc tcgtcctcct caaggatctt cacggtgccg gccgcgccgt ccatctcgac
     1621 acgggcgccg tcggggatac gggtggtcgc gtgaggaagc gcaactatgg tcgggatgcc
     1681 gaatttgcgc ccggagatgg cggtgtgcga cagcagggtg ccgcgctcga cgacgatgcc
     1741 gcgcgaggac agcatcagga acagccagcc gggatcggtc tcgcgggcga tcaggatcat
     1801 gtcctcggag gggtcgccga ggtcgtgcgg gtcgatgacg acgcgggcgg tgcctcgtac
     1861 gatgcctccg ctggagccca agccgcgcag ggtgcccgag gcggccgtgt cccggttcgg
     1921 gtccggcagc gcgtcccgta cggggcccat ggtcacgaag tccatgggca gttcggcgcc
     1981 ccgcgcgtgg aactcggccc ggcgtacggc ggccaggccc cgcaggtcgt cggtgacggc
     2041 cgtgccgtcg tatgtgccga ggatctcctc ctcggtcagg tgcatcacgt cctcggcctt
     2101 ctccagcaga ccgcgtgcgg ccaggtcggc gccgagcctg cggtatacct ggcggccgaa
     2161 tccgaacagc tcgctgcgac cgtagcgggt gttctcccgc atgttgatgt accggcgctg
     2221 cccggccagc gccgcgcgca gcgcgacgcc ggccagcggg cgccccgcga gcagctcggc
     2281 cagccgccgc tcgcccgccc ggcgggcctg ggcctcccgt tcgcgcaaca gggcgcgtgt
     2341 gaggtcgccg cgcgcgtact cggcggcgat gcgcagcagc tgggcgggct ggtcgcgggg
     2401 gaccggcacc tccagcttca gttcctgcag gccccggtcg ccgtggaggt ggttgtgggt
     2461 gcgcatccgg tcggcgaggt cactgccgta ggcgtcccgg tcgatctcgt cccacacctc
     2521 ctcggcgggc ttcaccgcga gcgcgtcgag gacgcccggc gtggcgcgta tccgttcggc
     2581 gatctcgacg atcgacatca ggatgatgac gctgcggttc tcctcgccgc cgcacagcag
     2641 atcgctgtgc agtccggcgt cggcctgcgg cacccagcgg ccgagcatct tcatggccag
     2701 cgtctcgtgc agttgcagca ggaggtcgtt gaccagcgtg atgccccagc ggtcaccgac
     2761 ctcggtccac aacgaccgca tcagctgcgc gagttgcagc ggatcggcgg tcttcgcggc
     2821 ctcccggtgc gcggtgagga tcctctccca ccaggtctcg aagtccttca ccgccgcgcc
     2881 gtgcgtcacc agccgccatg ccaggcgcgc cgtggccgcc gccgtcgcgg gcaacagggc
     2941 ggggtcggtg agcggcgggg tgggccggac gtgggtgacc gacggggaca tgcccatcat
     3001 ccgctcgaag cttctccgcc acagcgggaa ggtccggctc agtccgtgca aggcgtacca
     3061 gacgctcagc gagtagtaga cccgtccacg caacaggccg atcatgccgc gcagttcacc
     3121 ctcgtgccgg tcgaggacct tgcggggcac gccgagcagc cggtagaagt cgtggaagtc
     3181 ctcacggtag aagcgctggg cgaaggagta ggtcagtgcc gtggtgacgt ccgggtagga
     3241 ctcggtgatg ttggcgttgc tccacagccg ctgccggtcc agctccagca ccaccgggcg
     3301 ggcctggagg atgttcagcc ggccgtccgc ggtgaaggtg ccctcgatgt cctgcgggca
     3361 cccgaagagc tcctcgatcc ggtcgccgac catcacgacc tgttggatct gctcgtccgt
     3421 cagcgccggt gcgtcccaca gctcctcggg cgcgggcacc gtacgcgggc cgtcggcgag
     3481 ccggccgggg ttctcctcca tcagctgtgt cttgtgggcg agcacggacc gcaccgcgtt
     3541 gtcgcgccgg ctgacgaagt agtggtcgac cgggaccttc tcctggacga cgccctcacc
     3601 gatgccgagg ccggcagcga gcactcggtc gcgggcgccg gtcagcggat cgcaggtgaa
     3661 cagtacgaac gagcggcggc cgaactccat gcgctggacg ccgacggcca ccgcgaagcc
     3721 gtgctgtgtg ccgccccggg cggcccggta cagcagcgcc tgcgtcccga acccggaggc
     3781 ccagcactgc cgtacccggt ccaggagctc ctcgcgggtg acgtacagga agctgtcgct
     3841 gattccggcg aacgcgtcgt gcgccgagtc ctcgccgacg tccgcgcctg taccgacgac
     3901 cgaggagcgc accgagacca cggcatccgg cccgaagacc tcgtcgaagg cgtcgtacag
     3961 ctgccgtgcg actgcggccg gtacggaggc ggcgagcacc gtctcgcgca gcgcggacgc
     4021 ccttttctcc agagtctcgg cgtccgctgt gtccaagccg gcgagaaggg cgtccagctc
     4081 ggcgcgcaca gggcgcagca cttcggtgaa cgtggctgcc gacacgcaga agaagcgggg
     4141 gaccgggaag ccttccgttg ccatccgcgc ctgccgggag aacttcggac cgacctccgc
     4201 ctcgttcagc agggcccgcc cacgcatgac cggccccttc ctgcggtgcg acgagaacgt
     4261 ggggatgcgc agtgcgcggc gtgcgttcat cgagccgatc accatatcca ggggatgaca
     4321 cggcggtggg tcgccgcata gtcggagtag ccgggaagtg tgagcagggt ccgctcctcg
     4381 acgaggatgc gcacgaccag cgtcggcagc aggaccatga gggcggcggc actgtaggtg
     4441 ttgaggaaga acagcacgaa gacgacgtgg gcgagggcca tcccgagata ggcgggatgc
     4501 cgcacccacc tgtaggggcc gctctggacg acctgatggt cgccgagcac ccggacgcgg
     4561 tgcgaataga accggccaag ggtccggatg ccccacagcc ggaagccgac tgccgccgcg
     4621 aagaccacgg gcagcgcgag catccagggg tggtaggtct ggtcgtcggc cgcaggcaga
     4681 caggccgcgg cgacggtggc ggcacgggcg gtggcgtaga cgtagatggt gccgcggtcg
     4741 gtagccatct cgaccgaggt cgagctgcgg acggtcgtcc aagtttccag cagtatccac
     4801 atcagatgga gcccggcgag cgccaccgcc acccgctgcc agggagcgga gaggtcgagc
     4861 agtccgcgga ccacggccgt gccggtcgcc gcgacacata caccaaggat gaaaagggga
     4921 tatttacgca gtaaccacat tcgggggacc tccacggggc ggcgggggga tggccgacgg
     4981 actggaccgg ccgccggtga tcaggacgcg gcaccctggc cgagggtcag gtgctcgctc
     5041 acgccctgct tctccgcgta gagctccgcc agcgcgcggg tcgtcacgcg aatgtggtcc
     5101 gcctcgatct gatcggcccg ctgcccgtcg atcaccatca cgtccatcga gcccgacgcg
     5161 gcgaggacat ccgcctgcag caggtcgcac cggccccgga accgcagccg gttggggcct
     5221 ttgcgcttga gttcctccac cgtgtactcc acctgagtgg gcagcgggag caggaacttg
     5281 tggaacgtgg tctggaggga gctgatgacg aaccgacggg ccgtgtcgag cggtgccagc
     5341 agatgtcgct ccccgacggc gagcgtcatc tgccgtacgg cctcctggat gaccatgccg
     5401 gtcacatggg agccggtggc gtggtcgagg accatttcct ggcggtcgga gatgcgcagc
     5461 gtcgcccgga acaactggtc gtcgaccttg cgcagcccgg cgatgaggac gttctccgga
     5521 ttgtgtttgt gcacctcgtg acggtgcagc agcgtcggca gaggcaggtc catgagcatc
     5581 gactccggca ggccacgccg gcgcagtttg gcgcggacga tgtctcgctc gtccttctcg
     5641 atgccctgcc ccggcacgat caccagtggg ggatcggacg acagcccctc ttcaagaagt
     5701 aggtccaact gggataaggg caccagatct tctgaattcg acctgcaggc gagattgtcg
     5761 cctacgacga acacaaactt ctcggacatg accctccgcc ttcatattca cgtagttcgt
     5821 gagacgttta ggagggggtg atcttttcgg gcccgtattg tcgttcgttg gacgtggggc
     5881 ttgtggcagg tgcgacccca gggccccggc gtctgcctgg ctcgcccgac cacctcgacg
     5941 gtgcggacgc ctcagcttcc cagccgccga gctcgaccct gtcggggatc tcagcgcgcg
     6001 gcgtcggatg cggtgcatcg caaggcgccg ggtcgccctc ataccgggtg tattcgggcg
     6061 atccggcaac gccgcgaggc gccgtagctg tcgtcgtgcg cccgccggga gttacgggac
     6121 agcccttagg cgcgacgcgc acccgtgttg cgtacaggcg gatgaccgcc tcgtcccgca
     6181 ggaaccccgc ccaggtcttc gacagccccg cagtgatctt gttcaagtcc aggagcggca
     6241 acttcgcgag ccttcgagta aaggtgccga tgagcggcat gctcaacctc gaagaccgga
     6301 aacgacccac acgaaaaggg tccccgcagg ctctcgcctg cgaagaccct tcgctctgtc
     6361 gggacgacag gatttgaacc tgcgacccct tgacccccag tcaagtgcgc taccaagctg
     6421 cgccacgtcc cgatgcgcct cgctgcggtg aaccgcgtga agacgcaggt gaaccttacc
     6481 gcacctggac gggcggccgt cctcgccagg aggtccgtcg acgaacaggc cccgtgccgc
     6541 ggccctcgcg tcgaactcct ccaggtgggc ctgggcgtcg ggcatgcggt cgcacatcgc
     6601 ctccaggagg acctgcccca ggagcatggg cgcgctcacc gtgtcgaaga ccaggccggt
     6661 gcccacggcg gccgggagca gcatgtcgct gtgggccgcc accggggcgt acgtgccgtc
     6721 ggcgatcgtc accaccgtca gcccggcgtc ccgggcgtgg tcgagcgcgt ccaggagctc
     6781 gaccgggtgg cgcggcagcg cgaagcacag gagcgcgctc gcgcccgcgt ggcgggccgc
     6841 gtcgatccgg tcggcgagcg acgacccgcc ctcgtccagc aggcgcacgt cggggtgcac
     6901 cttcgccgcg aagtaggcga agccgcacgc ctgggcggcc gaggcgcgca acccgagcac
     6961 gggcagtgga cgggaggcgg cgagcagccg gcccgcggtt tccaccggtc cggggtcggc
     7021 gagcagggac gccagttgaa gcaggttgtc gatctcggcc cgtaccgcct gctggtaggg
     7081 gttggccggc cgtgcgtcca cggccggccc ggccggtacg acgtcccgca gatgtctgcg
     7141 cagcgccgga tatccgtcga agccgagggc gaccgcgaac cgggtcaccg acggctggct
     7201 caccccggcc agctgggcca gttccacgct cgaaaggaac ggcgcggccg ccgtccgccg
     7261 gaccaggcag tgggcgatac ggcgttgggt gggcgtcagt cggcgtccct cgaacagctg
     7321 gtacagccgc gagggagcga cagcggcccc gtgggacaca tcgacgccgg cgccttctcc
     7381 ggcgccctcc cctgctctct ccgtaccggc tctctcccta ccggctgcct ccgtgtcgct
     7441 caccctcgtc cccccagtcc gtcgtacgcg acccccgctc gcccgctcgt ccctgacacg
     7501 tgtggtccga ggtgcccgga cgacggcgcg ggtcagcttg tgaggccggc cgaggtgagc
     7561 cactccttcg ccacgtcgga ggcgtcctcc ttgtcgttga cgagcttctt catcatgtcc
     7621 agcaggtcct cggtggtgag cttggccgag aggtcgttga gcgccgactt ggccttgtcg
     7681 ttgaccgcgg acttgtagat gaggggcgtg acgttctggg aggagaagag gttcttcggg
     7741 tcctccagga ccaccagctt gtcctcgacg atggcggggt cggtcgtgta gaggttggcg
     7801 gcctggacct tgtcgtcctt cagcagcttc agcagggtgc tctgggcgcc cgcgtccagc
     7861 ggctggaact tgccgaactc gacgccgtag acggtcttca ggccgacccc gccctgggtg
     7921 cgcgtcttga actccgagcc tgcgccgatg gccaggtcct tcgcgaccgg cttgaggtcg
     7981 gccagggtct tgaggttgta cttgtcggcg gtctcggcgg tgaccgtgac cgagtccttg
     8041 tcctcggccg cggcggagtc gagtatcccc accgacttcg ggagcttctt cgtcagctcg
     8101 gcgttgatct cctccgtgct ggtggcggtg ctcttcttgt cgaccgccac ggacagcagc
     8161 gccccgttgt actcggggat gaccccgatg ccgcccttga cgacctggtc gtagaagacc
     8221 tcgcgggcgc cgatgtcgaa cttgcgggtc accttcaggc ccttgtcctc cagggcctgg
     8281 gcgtagatct cggcgagcag ctggttctcg gggaagttgg ccgagccgac gacgatggcc
     8341 ttgccgcctc cgctgtcggc gctgccgcct gtcagcgggt tgtcgtcact gtcgtcgctg
     8401 cctccgcagg cggtggcggt cagcgagagc gcggcgacga cgccgagggc ggcggcgcgg
     8461 tacgtggcct tgttcatggt gttccctttc tcaggtacgg ctctggtctc ggggcgggct
     8521 caggtctcgg ggcgggctct ggtgcgactt ccgcgcgcat cggatgcatc gggtgcgcgg
     8581 gagtccggtg gctcatgact tggtcgccgc gggccgcagc cccggcgaga cgatggcgcg
     8641 ccgcagcgcg gtgaagacgg cctggacgac gagcgccagt gccacgacga cggcggagcc
     8701 gccgatgacc agttcgtagt cgttgcggga gagcccgtcg acgatgaagc ggcccagacc
     8761 gccgagaccg ggatacgcgg cgacggtcgc cgtggccacc acctggatcg cggcgacccg
     8821 caggccgagc aggatcagcg gcagcgccat cggcacctcg accttcagca ggacctccca
     8881 gccggtcatg cccattccct tcgcggcgtc ccgggcgtcg gggtcgacgc cgcggacccc
     8941 ctcgaaggtg ttgatgagga tcggcgggac cgcgagcgcc accagggcga ccagcacggg
     9001 agtggtactg agcccggcga gggtcacgac gagtacgacc aggccgaagg tggggatcgc
     9061 ccgcgcgagg ttcgcgacgc tcgcgacggc gaacgcgccg cggccggtgt gcccgaccag
     9121 cagcccgaac gccagcccga tcagcgtcgc gaacagcagc gacagaccgc tgtaggtcag
     9181 gtgctccgcg agccggtgcg ggatgccgtc gtccccgtgc cactgctggg acgacgtcag
     9241 ccagtcgccc acgagccgga tctggttcag gagatcgttc acgccttcgc ccccttgccc
     9301 cgcgcccacg gtgtgagcag ccgctgggcc agtacgagca gcgcgtcggt gacgagggcc
     9361 agcagcatga tcagtacggt cgcggtgatg atcggcgtcg ggaagtcgag ctggaggccc
     9421 cggatgatgt agtagccgag gccgccctgg ccgatcagct gtcccaccgc gacgaggctg
     9481 atggaggaga ccgcggcgac ccgtaccccg gcgacgacga cgggtacggc gatcggcagt
     9541 tcgacctgga tgacccggcg gaccgtactg aagcccatcg cggtggccgc ctgccgtacc
     9601 ggatcgggca ccgaggccag tccgtccacg acgttgggta tcaggaccga cagcgtgtag
     9661 agggtcagcg ggatcatgac cgtgcgttcg gtcagccccg tgtagcggac gaagatcatg
     9721 aacagggcca gcgacgggac ggagtacagg acgttggccg cggtcagggt cggcgggtag
     9781 atccatcgcc agcggtggca gaggatgccc agcggcaccg agatgatcag cccgaacagc
     9841 accggcagga ggccgaggcg cagatggact ccggtgtagt gggccagttc accgacgtga
     9901 tcgccgatcc acacccaccg tatgagcggc tcaccgtctc tcatcggtcc ccaccgcctg
     9961 tcttgcctgt gttgctgccg gaaccgtcgg cgtcctccgc ggcggccggg tccgcggcgc
    10021 ctttgacgag ctgaggcccg gccgcctcgg cggtctcggc cacgccggcc gcttcgggcg
    10081 ccgcgccgag ggacccgccg gtaccggcga tcgccgccga cagctcgtac gcgtccgcga
    10141 ctcccgcgac cgcgccctgc gcgtccactc ccaccgccag ccgggagggt gacaggagcg
    10201 ccgcgtcgag ggcggcccgc gccgagtcgc cgaccaggct gaacgtgtgg ccgagcggtg
    10261 cgagcgggat gtccgccagc gtgccggcgt ccgcgaggcc ggcggcctcg gcccagccga
    10321 gcggccggcg ggcgtcgtcg acgacgagca cccagggctc gtccagggca cgggcctcgg
    10381 ccacggaggc ggtcaccggc agcgccgggc cctcgcgcag cggcagaccg gccgcctgga
    10441 cgaaggagag ccggcggata ccccggtcct gcccgacgaa gtcggccacg aagtcgtcgg
    10501 cggggtgcgc gagcaaccgc tccggggtgt cgaactgcgc gagcttgccg cccgtccgga
    10561 acaccgcgat gttgtcgccg agcttgatcg cctcgtcgat gtcatgggtg acgaacacga
    10621 tcgtcttgtg cagttccttc tgcagccgga ggaactccgc ctgcagttcc ccccggacga
    10681 tggggtcgac cgcgctgaac ggctcgtcca tgagcagcac gggcgggtcc gcgcccagcg
    10741 ccctggccac cccgacgcgc tgctgctgcc cgccggagag ctggtacgg
//
