LOCUS       NZ_AP023438            20726 bp    DNA     circular CON 15-DEC-2024
DEFINITION  Micromonospora sagamiensis strain JCM 3310 chromosome, complete
            genome.
ACCESSION   NZ_AP023438
VERSION     NZ_AP023438.1
KEYWORDS    RefSeq.
SOURCE      Micromonospora sagamiensis
  ORGANISM  Micromonospora sagamiensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Micromonosporales; Micromonosporaceae; Micromonospora.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 6930736)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu
            Genome Coverage                   :: 62X
            Sequencing Technology :: Illumina HiSeq X Ten; PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014680085.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 15:12:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,941
            CDSs (total)                      :: 5,859
            Genes (coding)                    :: 5,751
            CDSs (with protein)               :: 5,751
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 30 of 108
            Pseudo Genes (incomplete)         :: 88 of 108
            Pseudo Genes (internal stop)      :: 5 of 108
            Pseudo Genes (multiple problems)  :: 15 of 108
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:39:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4337496
            Orig. end                         :: 4358222
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023438.1.
            Genome sequencing and analysis were conducted under the Global
            Catalogue of Microorganisms (GCM) 10K type strain sequencing
            project (http://gctype.wdcm.org). The genomic data are also
            available at http://gctype.wdcm.org/sequencing/GCM10017556
            Annotated by DFAST v.1.2.7 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20726
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="21"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20726
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20726
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10726](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10726)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            76..270
                     /locus_tag="IGA62_RS19040"
                     /old_locus_tag="GCM10017556_37970"
     CDS             76..270
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018722689.1"
                     /locus_tag="IGA62_RS19040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_37970"
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_145819499.1"
                     /transl_table=11
                     /translation="MKALDSQWRTSSRSGGNGACVEARYADHATQVRDSKDRQGPVLVF
                     GPGQWIEFIKGVRAGAVGA"
     gene            complement(293..1456)
                     /locus_tag="IGA62_RS19045"
     CDS             complement(293..1456)
                     /EC_number="3.1.21.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013579.5"
                     /locus_tag="IGA62_RS19045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="restriction endonuclease subunit S"
                     /protein_id="WP_186500006.1"
                     /transl_table=11
                     /translation="MVRLGNIGAGHFKDSDSAYIPHEYFRELQRHEIKTGDLLIAGLGD
                     ERNPVGRACIAPTNLGPAIVKADCFRARLDQSRMTHRFAAWALSSSFITDQVATLSRGS
                     TRTRINLDIARHIQIPVPDIEEQRRIADFLDAATSRLDRLARRRVYQAALLSAYHRSWL
                     SEEWESLVKEFGKARLRHLVTSLEQGWSPQCEERVVEDDGWGVVKAGCVNTGVFDPNQH
                     KALPANMEPRREYKLEVGDLLVSRASGSPDLIGSTAVVDQLTGKLLLCDKIYRLKVDKT
                     KALPWFVAFMLRSQAAREHIKRGISGAEGMANNLPTSIVKDCQIPNAPLRKQDHVVRKA
                     TEHRDSIEKTRSALQEASRLISERRQALITAAVTGQIDVSTASGQAE"
     misc_feature    complement(1235..1237)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1576..3627)
                     /locus_tag="IGA62_RS19050"
                     /old_locus_tag="GCM10017556_37990"
     CDS             complement(1576..3627)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018732251.1"
                     /locus_tag="IGA62_RS19050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_37990"
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="WP_145819497.1"
                     /transl_table=11
                     /translation="MSSTSKHTELANHAWSVADLLRGDYKQSDYGKVILPFTVLRRLEC
                     VLAPTREQVRQTYEKHRGQDVDVSRFLRRAAGHSFYNVSGYTLKSVAADPSQTAKHLLA
                     YIGAFSDNAQEVLHRYEFPQQIRRLDAANLLYRVVARFADLDLDPVRRDPQTGEVRLDE
                     RGQPVVNVSNHQMGYVFEELIRRFAEQSNETAGEHFTPREVIELMVNLLVAPDHDTLSV
                     PGMSLSVMDPACGTGGMLSAAEEHITALNPNATVTVFGQELNPESWAICRSDMLIKGQD
                     PENIKFGNSFSDDGHRGAHFDYLLANPPFGVEWKKVKDEVEAEHEKLGDSGRFGAGLPR
                     INDGSLLFLQHMISKMKPVTANGRGGSRVAIVFNGSPLFTGAAESGESRIRQWILEADL
                     LEGIVALPDQLFYNTGISTYFWILTNRKLPDHKGKVILLDAREHFAKMRKSLGDKRKYL
                     TEDQITDITQLYGEALHVAADPEHPLHAKVKVFANEDFGYRRITVERPLKLRFEVTEET
                     LTAVRESKPIGKALDVTAFETALKPLVGRSWDTKRAAWDAVRTAMAEAGVLWPSGAPFQ
                     KALRDVLGVRDPDGEVQKVKGEPEPDTDLRDYENVPLHEDVEEYLRREVLPHVPDAWID
                     HTKTKIGYEIPFTRHFYVYKPPRPLAEIDAELKALEAEIQALLGEVTK"
     gene            complement(3624..5756)
                     /locus_tag="IGA62_RS19055"
                     /old_locus_tag="GCM10017556_38000"
     CDS             complement(3624..5756)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458730.1"
                     /locus_tag="IGA62_RS19055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_38000"
                     /product="N-6 DNA methylase"
                     /protein_id="WP_186500004.1"
                     /transl_table=11
                     /translation="MPQSALVTAAEISRLAGVTRATVSNWRRRHPDFPAPTGGTEASPA
                     YDLAAVRTWLANRGQLPDSSPADELRTALRAAEADHAARTRLLPLVLAASRLPEEARRT
                     LAELPDHRLPAGAREAARPCAGQLPDIGEPTYRADEVPLLRALLRCVAQAGALPTLDVL
                     AEGTVDDTSTSGAYLTPAPVAELMAGLLAEPGEAYPEQVLDPACGAGGLLAAAATRGAR
                     ELSGQDLAPAQAAQAAVRLLLLAGDATVRVRVGDSLRDDGYPSVTADAVLCAPPYGQRD
                     WGHDDLAYDSRWVFGLPPKGESELAWLQHCLARLAPGRRAVLLLPPATAERPSGRRIRA
                     EMLRHGALRAVVALPAGVAPPLHVGLHVWLLQRPHPQAATPGHVLFVDTAAPPEPHRAG
                     EPESRRPTVSDWSAVRATALAAWADFDRHPDAFDPIPGTARAVAVIDLLDEAVDLTPAR
                     HVRTPVPVRPDELAEAAYGLRRRLRRAVSGLSALSGGGDWPPAGATARTWRTATVADLL
                     RGEALTLLRAPAATRGPQPADRDTATPRTLTARDVLAHRPASGAADDDPAGAEVPIEVG
                     DVVLPELLHHGGGTARVADARDAGLFLGRHLYLLRPDPARLDPWFLAGFLAAEDNLTAA
                     SSGTSVIRVDPRRLRVPLLPLAEQQRYGRAFRQLNAVRVAADLANRLAGETARTLAAGL
                     TGGALLPPDSPVEGTR"
     misc_feature    5813..5824
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.44"
                     /tool="antismash"
     gene            5946..8417
                     /locus_tag="IGA62_RS19060"
                     /old_locus_tag="GCM10017556_38010"
     CDS             5946..8417
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014516.5"
                     /locus_tag="IGA62_RS19060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_38010"
                     /product="AAA family ATPase"
                     /protein_id="WP_186499999.1"
                     /transl_table=11
                     /translation="MSDPAVHDPDIVGQLVLDQLADAALPETAENLIVAALLGESYLAD
                     QLGGAAPPQRPRPTPTDPDDSGPVGTYLADIEVTGFRGIGTTATLNLTARPGLTIVTGR
                     NGSGKSSFAEAAEFALTGDNKRWSERSTVWKKGWRNLHTEGGSCIRVRLNVDGHRKPAT
                     VECRWTAGADLGDHTDSLQFLGEQRTSVAELGWSRHLELYRPFLSYSELGGLINGKPSE
                     MHDSLDGILGLKRVAEIEKLLKAARKDTDQRRRRAAEELPALRALLTEHPDPRARRAEE
                     ALAGRTADLDTLDEVATSGAPDDDATVVPLRQLAVLDLPNRDDLAARADRLRAALRHVG
                     ELAGTPAEQARALAGLLRAALAHHDNHPDQPCPVCGGRTLDEAWAVQARTQVRELTLRA
                     EQLDAAHRAGHEARHALRQAVPSRPPVLTVDPGPDGIDLTELRKAWQRWHDLTADTDPA
                     MLVELAPTTFDDLAAALAPVRAAAREALQRRRQDWQPVADRIRAWIDIERASRQAAIVL
                     PDLRKAIEALKTIGATIRAERLRPIAAEATAIWNTLRQDSNVELDAIRLAGSGPARRVD
                     LDVRVDGVPDAALSVMSQGELHSLALALFLPRATTAESPFRFLVIDDPVQSMDPAKVHG
                     LAQVLHRVAATRQVVVFTHDDRLPAALRHLRLDARILTVSRRDHSQVTVDTGKDPAERY
                     LKDAQGIADDEDMSEQTRITVACNLIRDALEYVCHERIRIAGLRAGTPFADIEDTIAEA
                     QSDGLRPLLALALLGDHRRTDEVDVELRKVDSAAVRLVREVNRGAHGNAVPNLHTLVAD
                     GRRVAGRLSRP"
     gene            8414..8767
                     /locus_tag="IGA62_RS19065"
                     /old_locus_tag="GCM10017556_38020"
     CDS             8414..8767
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IGA62_RS19065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10017556_38020"
                     /product="hypothetical protein"
                     /protein_id="WP_145819495.1"
                     /transl_table=11
                     /translation="MTPQHLLDQAADLLVNPRPSMRRCWQRGSACLTRVALEQGMRDYW
                     STIDVNVGRCRMSRQLLALRAFAGDETALIARTAWHGLSRAMHHHTYELAPTLAELRGW
                     HRDVTDLLPRLKP"
     gene            8879..9910
                     /locus_tag="IGA62_RS19070"
                     /old_locus_tag="GCM10017556_38030"
     CDS             8879..9910
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IGA62_RS19070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10017556_38030"
                     /product="hypothetical protein"
                     /protein_id="WP_145819494.1"
                     /transl_table=11
                     /translation="MKLRARLAAALAVTLTAGVVAVPSPALAWAQLNNDPDFIQVYVNN
                     IENLETVDAVNCPGDWEDLIYYMKTYETSPDLFLVQQLAGPGELNTLLDRMNTLLAGEF
                     AGILAEPDPEQMQSPCGAEKDHQTNAIIWRIGRFDMVAGSQLTWQSLAEDASGTCAPNH
                     QDRTINVAARLYDKIAGRYVSAGSIHWPTGNSDGPACAPENAARTVEKVESIGGALRVW
                     GGDANVSDQANGDWRSWYATTNGQLGGRHNYRDAAFDDCSERVDGGTALRQCLRDEHWT
                     VGGDNRIDVLFARRGDGSMPMTGAEHTVTFAEGDQADIAVTGSDRQDRSYSDHRAIRAR
                     VHY"
     gene            complement(10001..10726)
                     /locus_tag="IGA62_RS19075"
                     /old_locus_tag="GCM10017556_38040"
     CDS             complement(10001..10726)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073312.1"
                     /locus_tag="IGA62_RS19075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_38040"
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_145822131.1"
                     /sec_met_domain="CDPS (E-value: 9.1e-72, bitscore: 239.7,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAEGATNQDGNTPIRQGLFVVEPFTDDCRLIWEQRQHAVLGVS
                     PGNSYFNVARLTDLLGWLCAEFARVDVVIPDSALKHTYLALGYEPDRADKKARGETNVL
                     RNRVVRAWESSGGPRATDGLNRMSDLVSNEVYRRKLAECEQTLNEDGILWETCAEMSRD
                     VLAARGYDGPLDTERIERAMRYLVAELPFFLASADIFDASSSLNFYHQRLPLAELIFSG
                     KSLLQASPRQAYATIHPVP"
     gene            complement(10726..12267)
                     /locus_tag="IGA62_RS19080"
                     /old_locus_tag="GCM10017556_38050"
     CDS             complement(10726..12267)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (22-410). E-value: 6.4e-55.
                     Score: 178.3. Matches aSDomain:
                     nrpspksdomains_IGA62_RS19080_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.5"
                     /locus_tag="IGA62_RS19080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_38050"
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="WP_145819493.1"
                     /sec_met_domain="AMP-binding (E-value: 1.8e-48, bitscore:
                     163.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFRPNRPLDIDPSGQAVLDIEAIAQCVRKTAGALSAAGVRPGDR
                     FAVYKDNHFDCLLLAAAGIRIGAIPVMLSGLLPTDSTRALLQRVEPRLLVSNRQLLENR
                     GSDGSSLTSFAERTLCLDEGVPDTLAFADLYGSTDPSPVARRDDELMMLTHTSGTTGIP
                     KLIMYTPAKLQGQMARLECRHLPPITFHRNDTVAMFTPYVHARAFTWIYSVLTLSPTKV
                     LVMSDSDPEVVGPLFQQHRPTVVEALPIDYFRMEGLARDPRNDAFSSVRMFINNFDAVR
                     WATIRPYLNASRHPFPLWREGYGQSETGGMGMTFVTRRTANIRRDQRPGPRKVGRPMPG
                     FVQLKVVDPKTFEPVPDGQPGLVLARTRARCAGYYQEPERWNEKAVGEWWNTGDIGIKT
                     RTGALKLIDREVNYAPGLNCLEQEDVIVDNLPAGHDVALLPVSDGPPVPIVATPDGQLD
                     GEQWRNAVRKLPELAEPIVIAFKDMPRTGTGKIIREELRRRYLNGAAHPGTGRWT"
     aSDomain        complement(11038..12201)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IGA62_RS19080_AMP-binding.1"
                     /evalue="6.40E-55"
                     /label="IGA62_RS19080_AMP-binding.1"
                     /locus_tag="IGA62_RS19080"
                     /protein_end="410"
                     /protein_start="22"
                     /score="178.3"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="AIAQCVRKTAGALSAAGVRPGDRFAVYKDNHFDCLLLAAAGIRIG
                     AIPVMLSGLLPTDSTRALLQRVEPRLLVSNRQLLENRGSDGSSLTSFAERTLCLDEGVP
                     DTLAFADLYGSTDPSPVARRDDELMMLTHTSGTTGIPKLIMYTPAKLQGQMARLECRHL
                     PPITFHRNDTVAMFTPYVHARAFTWIYSVLTLSPTKVLVMSDSDPEVVGPLFQQHRPTV
                     VEALPIDYFRMEGLARDPRNDAFSSVRMFINNFDAVRWATIRPYLNASRHPFPLWREGY
                     GQSETGGMGMTFVTRRTANIRRDQRPGPRKVGRPMPGFVQLKVVDPKTFEPVPDGQPGL
                     VLARTRARCAGYYQEPERWNEKAVGEWWNTGDIGIKTRTGALKLIDRE"
     CDS_motif       complement(11764..11814)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IGA62_RS19080_0001"
                     /evalue="2.00E-03"
                     /label="NRPS-A_a3"
                     /locus_tag="IGA62_RS19080"
                     /protein_end="168"
                     /protein_start="151"
                     /score="11.2"
                     /tool="antismash"
                     /translation="MLTHTSGTTGIPKLIMY"
     misc_feature    12383..12403
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.78"
                     /tool="antismash"
     gene            complement(12627..13571)
                     /locus_tag="IGA62_RS19085"
                     /old_locus_tag="GCM10017556_38060"
     CDS             complement(12627..13571)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464995.1"
                     /locus_tag="IGA62_RS19085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_38060"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_145819492.1"
                     /transl_table=11
                     /translation="MMDDSAEFGRRTFLLAAAAVTVAGPAGAAVPAGATGPGRGSRASG
                     ARSVGAYEVIPLRDATGTFFLDRQAAFPAATSDDWELARAVDPGAFGPGDAWQLDFRCY
                     AVRGPRGRLTLVDTGVGPDGSPAEGWAPTPGRLPAELDRAGIDPADVDTVVLTHLHEDH
                     FGWSVGLDGTPAFPNARYVVQRREVATLPDGDAARTYVVEPLRATGQLHEVDGASRLHS
                     ARGRGGCVRVVPTPGHTPGHQSVVVEDGHDQVIVTGDVLVHAVQLVDPAVGYRYEADPE
                     AARETRQRLLDEARARRAVLATAHLNQPWTALG"
     gene            13808..14791
                     /locus_tag="IGA62_RS19090"
                     /old_locus_tag="GCM10017556_38070"
     CDS             13808..14791
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014068.5"
                     /locus_tag="IGA62_RS19090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_38070"
                     /product="GlxA family transcriptional regulator"
                     /protein_id="WP_145819491.1"
                     /transl_table=11
                     /translation="MPLDVAMLAYPGGGSFDYVTVNEVFGVDYSHRFGSWYRTRACGLT
                     PGPVELDGGVIVQAGCGLDEAERADLVVVPGWTREHEVPPRELLATLRGAYRRGARIMS
                     ICTGAFVLGHAGLLDGRRVTTHWAMAPLLASLFPAATVEPRALFVDDRRVLTAAGMSAG
                     MDLALHVVRGDFGADAAAALARRLVLAGYRSGGQAQFVDAPLGPEPDDPLPDLLDWLRQ
                     HLDQRWSLGSLGARVHMSPRTLSRRFHRLTGMSPHQWLLRERILAAQRLLESGDEPVAQ
                     IAVRTGMGSAGNLRHSFRRELGVSPQAYRTSFRAGGPRAGARSSGP"
     gene            complement(14881..15891)
                     /locus_tag="IGA62_RS19095"
                     /old_locus_tag="GCM10017556_38080"
     CDS             complement(14881..15891)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621327.1"
                     /locus_tag="IGA62_RS19095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_38080"
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="WP_145819490.1"
                     /transl_table=11
                     /translation="MNHSSITPRRRGRLRLLLGALCAAVLAVTGTVVAAPHAFAEADRY
                     LSSNTTGTHNGYYFSFWKDSGSASMTLRENGRYTSSWDRSTNNWVGGKGWATGTRRTVS
                     YSGTYNPGNNNTYLALYGWTRNPLIEYYVVENFGSYNPSSGAQRLGTVTTDGGTYDILR
                     SQRVNAPCIDGNNCTFYQYWSVRQQKRSSGTITTANHFDAWARAGLNLGTHSYQIMATE
                     GYQSSGSSDITVWEGGGTPTTGPTSPGGNCTAVLSAGERWGDRFNLNVAVSGTNNWVVT
                     LNLNGGQWIQNSWNASVSGTSGTITATPNGNGNNFGITIMANGNWTWPTVSCRTA"
     misc_feature    16065..16079
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     misc_feature    16125..16139
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.95"
                     /tool="antismash"
     misc_feature    16126..16140
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.01"
                     /tool="antismash"
     misc_feature    complement(16258..16272)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.6"
                     /tool="antismash"
     gene            complement(16347..19280)
                     /locus_tag="IGA62_RS19100"
                     /old_locus_tag="GCM10017556_38090"
     CDS             complement(16347..19280)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013124.5"
                     /locus_tag="IGA62_RS19100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_38090"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_145819489.1"
                     /transl_table=11
                     /translation="MAGRLTFGLLGPVQIRSDGQPVPAGPPRQRAVLAALLLHVNRITP
                     VDDIVDLLWGDEPPRTARKNVQLHVWRLRQLVGDRLASDPPGYRITVDPDDLDVSRFDI
                     LLESARAAVRAGPTGRAATLYRSALDLWRGAPLADVAGLGGFGGSVAWLDRRRLSAYEE
                     YVDVELDLGRHDEMLAVLDQLVARYPLHERFAEQQVMTLHHLGRRSEAVDAYRRYRRAL
                     ADELTMEPGPVLRRLAELARQPTPPAAVPARPPATAVRELPHTAPVLVGRDRPLAALTA
                     ALTTGPRHRMPLYVVTGPAGVGKSALAVQAAHRVATAFPDGQLYVDLRGATAGVCPLPP
                     HSALGRFLRVLGVAETAIPTDVAEASAALRSLLAGRRVLLVLDNAHDAGQVRPLIPAGD
                     GCAVLVTSRRKLTDVDGATHLELDILAEADAVDLLRRLAGDARVDAEADTAARLVRRLG
                     GLPLAVRIAGARLAARPAWSLATLDDRLRDEHRRLHELRQGDIAVRTSFLVSYQHLTHP
                     LAPRLFRLVGAVDSVDVTGEVAAALLDVDLPPALDALDELVDARLLDETTAGRYAMHDL
                     IRLFARERAAEEPEPVPTAAVHRMLAHHLERTRQALGLLRPGTRVPTVAGPSAFDDAPA
                     ALAWLSAERPNLVAGVVQAARAEATARAGVDLADSLFLFFEMRGHVDDWITVDRAGVAA
                     AERLGDPVAQARLLHDLAVAHFHRNRTDEATHLLRRSLDLSDAAGDDEGQARALNQLGV
                     LHGMAGAYDESAACFKRSLRLRRRLGDLRSCAATTGNIGMAYRLAGRYEESIRFCRTAV
                     RLARRSNAPEIEANALGNLGEVQCLLGRHHSALRYLRRGLAVAGVPVNERNRGSMMGTL
                     ADAYLGAGQVDSAIRYAELAVAAMRQAEFRHGEAVALRRLGLMLCRHGDVDRGVRQLRL
                     ALAVFTDVGSPEADEVRAELRSRFGLRNDDVPTPSPGERVNRGPTPS"
     gene            19370..20095
                     /locus_tag="IGA62_RS19105"
                     /old_locus_tag="GCM10017556_38110"
     CDS             19370..20095
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IGA62_RS19105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10017556_38110"
                     /product="hypothetical protein"
                     /protein_id="WP_145819488.1"
                     /transl_table=11
                     /translation="MSDRTTGERLPAWVPAMWAAVLFAALLTPAGPAHASHPNPSSTRH
                     LHNMHYASSGTSAGANDEQFCVESTDTAKVSHSSARTFVKQTLTEMGAGKVWDGTGNWR
                     IDLWATNSNCTAYPASTRSTIEIEVHYGWDWSRFCGAPTGYYNCVVHDNPVWDAAHGHY
                     DSQWAYVYLVFSSGGRLDNVGRAFINHEFGHVFGLADDNGVCNPPSLMHSSIVGYSCTN
                     WPYWYPSPSDFQSVVNVMA"
ORIGIN
        1 cagccttcga gccgcagccc tgtctcctac ggcctcgact gcgatgatca agaggattcg
       61 ggacgaggag actccatgaa ggcgctcgac agccagtggc gcaccagcag ccgcagcggc
      121 ggcaacggtg cgtgcgtgga ggcccggtac gccgaccacg ccacccaggt ccgggacagc
      181 aaggaccgac aggggccggt gctggtgttt ggccccggcc agtggatcga gttcatcaag
      241 ggtgtccggg ccggtgccgt cggggcctga acagcgaagc agaaggccgg ctctactctg
      301 cctgcccgct cgccgtcgat acgtcgatct gcccggtcac tgctgctgtg attagcgcct
      361 gccggcgctc cgaaatcagg cgacttgcct cttgaagagc tgagcgagtc ttctcgatgc
      421 tatcccgatg ttctgtggct ttccggacta cgtgatcctg tttgcggagt ggggcgttgg
      481 ggatctggca gtccttaacg atggacgtag gaagattgtt ggccattcct tctgctcctg
      541 atatccccct tttgatgtgc tctcgggcag cctggctgcg tagcatgaac gcgacgaacc
      601 agggtagagc tttcgtcttg tcgactttca aacggtaaat tttgtcgcat agtagcaatt
      661 ttcccgtgag ttgatcgact actgcggtgc ttccgataag gtcgggagac ccactcgcgc
      721 gcgataccaa caagtctccg acctcaagtt tgtattcacg ccgaggctcc atgttcgctg
      781 gtagtgcctt gtgctggttg ggatcaaaga cgccggtgtt aacgcagcca gccttgacca
      841 cgccccaccc gtcatcctcg acaactcgtt cctcgcactg gggggaccag ccttgctcga
      901 gggaagtcac gagatgtcga agtcgcgctt taccgaattc cttgacaagg ctctcccatt
      961 cttctgagag ccagcttcga tgatatgcgc tcaacagtgc ggcttggtag acccttcttc
     1021 gtgccaagcg gtcgagtcgg ctggttgcgg catcgaggaa gtcggcgata cgccgctgct
     1081 cctctatatc ggggactgga atctgtatgt ggcgtgctat gtcgagattg atgcgggtgc
     1141 gcgtggatcc acgggatagt gtggcaacct gatccgtgat gaaagaggag ctaagtgccc
     1201 aagcggcaaa gcgatgcgtc attcggcttt gatctaaacg cgctcgaaag cagtcggcct
     1261 tgacgattgc tggaccaagg tttgttggtg cgatgcatgc tcggccgaca gggttccgct
     1321 cgtcacccag gcccgctatc aggagatctc ccgtctttat ctcatgtcgc tgcagctctc
     1381 gaaaatattc gtgcgggatg taggctgagt cggagtcctt gaagtgccca gcgccgatgt
     1441 ttccgagcct gaccacgcga gcgccactgt ccgaatagtg gctactggtc aatgcgcttc
     1501 cgaatgggcc atctgctatc tcgagtatga atcgcctgat cggaaagagt tgaaaaggga
     1561 tcgagcttga ggggttcact tcgtcacctc gcccagcagt gcctggatct cggcttcgag
     1621 ggccttgagt tcggcgtcga tttccgccag gggcctcggg ggcttgtaga cgtagaagtg
     1681 gcgggtgaag gggatctcgt acccgatctt ggttttggtg tggtcgatcc aggcgtccgg
     1741 gacgtgcggg agcacctcgc ggcgtaggta ttcctcgacg tcctcgtgca gcggcacgtt
     1801 ctcgtagtcg cgcaggtcgg tgtccggctc gggttcgccc ttgaccttct gcacctcgcc
     1861 gtccgggtcg cggacgccaa ggacgtcgcg gagcgccttc tggaagggtg cgccgctggg
     1921 ccagagcacg ccggcctcag ccatcgccgt gcggacggcg tcccaggcgg cccgtttggt
     1981 gtcccacgag cggcccacca gcggcttcaa cgccgtctcg aaggcggtga cgtccagcgc
     2041 cttgccgatc ggcttcgact cgcgtacggc ggtcagggtt tcctcggtga cctcgaagcg
     2101 gagcttgagc gggcgttcga cggtgatccg gcggtagccg aagtcctcgt tggcgaagac
     2161 cttcaccttg gcgtgcagcg ggtgctccgg gtcggcggcg acgtgcagcg cctcgccgta
     2221 gagctgggtg atatcggtga tctggtcctc ggtgaggtac ttgcgcttgt cgccgaggga
     2281 cttgcgcatc ttggcgaagt gctcccgggc gtccagcagg atgaccttgc ccttgtggtc
     2341 gggcagcttg cggttggtga ggatccagaa gtacgtggag atgccggtgt tgtagaagag
     2401 ctggtcgggc agcgcgacga tgccttccag caggtctgcc tccaggatcc actggcggat
     2461 gcgggactcg cccgactcgg cggccccggt gaacagcggc gagccgttga agacgatggc
     2521 gacccggctg ccgcccctgc cgttggcggt gaccggcttc atcttcgaga tcatgtgctg
     2581 gaggaagagc agcgagccgt cgttgatccg gggcaggccg gcaccgaacc gtccgctgtc
     2641 gccgagcttc tcgtgctcgg cctcgacctc gtctttgacc ttcttccact ccaccccgaa
     2701 cggcgggttc gccagcaggt agtcgaagtg cgcgccccgg tggccgtcgt cgctgaacga
     2761 gttgccgaac ttgatgttct ccgggtcctg gcccttgatc agcatgtcgg accggcagat
     2821 cgcccaggac tccgggttca gctcctgccc gaagaccgtc accgtcgcgt tcgggttgag
     2881 cgcggtgatg tgctcctcgg cggcgctgag catgccgccc gtgccgcagg ccgggtccat
     2941 gaccgacagg gacatgccgg ggacgctcag cgtgtcgtgg tctggggcga ccagcaggtt
     3001 caccatcagc tcgatgacct cgcgcggggt gaagtgctca ccggccgtct cgttggactg
     3061 ctcggcgaac cgccggatca gctcctcgaa gacgtacccc atctggtggt tggagacgtt
     3121 gaccaccggc tggccgcgct cgtcgagcct gacctcaccg gtctgcgggt cgcgcctgac
     3181 cgggtccagg tccaggtccg cgaagcgggc gaccacccgg tagagcaggt tcgcggcgtc
     3241 caggcggcgg atctgctggg ggaactcgta gcggtgcagc acctcctgcg cgttgtcgga
     3301 gaacgcgccg atgtaggcga gcaggtgctt ggcggtctga ctcgggtcgg ccgccaccga
     3361 cttcagggtg tagccgctga cgttgtagaa gctgtgcccg gcggcgcggc gcaggaaacg
     3421 gctcacgtcg acgtcctggc cccggtgctt ctcgtatgtc tggcgcacct gctcccgggt
     3481 cggcgcgagg acacactcca ggcggcgcag caccgtgaac ggcaggatca ccttgccgta
     3541 gtcggactgc ttgtagtcgc cgcgcaggag atcggcgacc gaccaggcgt ggttggccag
     3601 ttcggtgtgt ttgctggtgc tgctcaacgg gttccttcca caggggagtc cggggggaga
     3661 agcgcgccgc cggtgaggcc cgcagccagg gtacgggccg tctcgccggc aagtcggttg
     3721 gccaggtccg ccgccacgcg tacggcgttg agctgccgga acgcccggcc gtagcgctgc
     3781 tgctcggcca gcggcagcag cggcacccgc agccggcgag ggtccacccg gatcaccgac
     3841 gtgcccgacg acgcggcggt caggttgtcc tccgcggcga ggaaaccggc caggaaccac
     3901 ggatccagcc gggccgggtc gggacgcagc agatagaggt gccggccgag gaacagaccg
     3961 gcgtcccggg cgtccgccac ccgcgcggtg ccgccgccgt ggtgcagcaa ctcgggcagg
     4021 acgacgtccc ccacctcgat cgggacctcc gcgccggccg ggtcgtcgtc ggcggccccg
     4081 gaggcgggac ggtgggcgag cacgtcccgc gcggtcaggg tgcgcggggt cgcggtgtct
     4141 cggtcggccg gctggggacc gcgggtggcg gccggcgcgc ggagcagcgt cagcgcctcg
     4201 ccgcgcagca ggtcggccac ggtcgcggta cgccaggtgc gggcggtcgc cccggccggc
     4261 ggccagtccc cgccgccgct gagcgccgac aggccggaca ccgcccgccg cagccggcga
     4321 cgcagtccgt acgcggcctc ggccagctcg tccgggcgga ccggcaccgg ggtccggacg
     4381 tgccgggccg gggtcaggtc caccgcctcg tcgagcaggt cgatgaccgc gaccgcccga
     4441 gccgtgccgg ggatcgggtc gaaggcgtcc ggatgccggt cgaaatcggc ccacgccgcc
     4501 agggcggtgg cgcgcaccgc cgaccagtcc gagaccgtgg gacggcggct ctccggctcg
     4561 ccggcgcggt gcggctccgg tggggcggca gtgtccacga acagcacgtg gcccggtgtg
     4621 gcggcctgcg ggtgcgggcg ttgcagcagc catacgtgca ggccgacgtg cagtggtggc
     4681 gccacccccg ccggcagggc gacgacggcg cgcagcgccc cgtgccgcag catctccgcc
     4741 cggatccgtc ggccggacgg gcgctcggcg gtggccgggg gcagcagcag gacggcccgt
     4801 cgaccgggag ccaaccgggc caggcagtgc tggagccagg ccagctccga ctcgcccttc
     4861 ggaggcaggc cgaacaccca ccgggagtcg tacgccaggt cgtcgtgccc ccagtcccgc
     4921 tgcccgtacg gcggtgcgca gagcaccgcg tcggcggtga cggacgggta cccgtcgtcg
     4981 cggagggagt cgccgacgcg tacccggacg gtggcgtccc cggccaggag gaggaggcgt
     5041 accgctgcct gggcggcctg ggccggcgcg agatcctgcc cggacagctc ccgcgcgcca
     5101 cgcgtggcgg cggcggcgag caggccgccg gccccgcacg ccgggtcgag cacctgctcc
     5161 ggatacgcct caccgggctc ggcgagcagg ccggccatca gctccgccac cggggccggg
     5221 gtcaggtacg cgccgctggt gctggtgtcg tcgacggtgc cctcggcgag cacgtccagg
     5281 gtgggcaggg cacccgcctg ggcgacgcag cgcagcagcg cccgaagcag gggcacctcg
     5341 tcggcgcggt aggtcggctc gccgatgtcg gggagctgcc cggcgcacgg ccgggcggcc
     5401 tcgcgtgccc cggcggggag ccggtggtcg ggcagctcgg ccagcgtgcg gcgggcctcc
     5461 tcgggcagcc ggctggcggc gaggaccagg ggaagtagcc gggtccgggc ggcgtggtcc
     5521 gcctcggcgg cgcgcagggc ggtacgtagt tcgtcggcgg gggagctgtc cggcagttgc
     5581 ccccggttgg ccagccaggt ccgcacggcc gccaggtcgt acgccgggct ggcctcggtg
     5641 ccgccggtgg gggcggggaa gtccgggtgc cgccgccgcc agttgctcac cgtggcgcgc
     5701 gtgacgccgg ccagccggga gatctcggcg gcggtcacca gggctgactg cggcatcggc
     5761 gtcctgtcga ctcgggtacg gggacggtgg cactgtacac gagtcgatcc gcgtaaacga
     5821 tttgacagtg ctttcagtgc agaggtttgc tagtgtcgct ttcaccgttg gtcgagggtg
     5881 cccgacggtg cactccctcc ccaccggccc gtcgaacggg cccgtcatcc ccaccggagg
     5941 accccgtgtc cgatcccgcc gtccacgacc ccgacatcgt cgggcagctc gtcctcgacc
     6001 aactggccga cgccgcgctg cccgaaaccg ccgagaacct gatcgtcgcc gccctgctcg
     6061 gcgagtccta cctggccgac cagctcggcg gggccgcacc cccgcagcgg ccccgaccca
     6121 cgcccaccga ccccgacgac tccgggccgg tcggcaccta cctcgccgac atcgaggtca
     6181 ccggcttccg gggcatcggc accaccgcca ccctcaacct gaccgcccgc cccgggctga
     6241 ccatcgtcac cggccgcaac ggctccggga agtccagctt cgccgaggcc gccgagttcg
     6301 ccctcaccgg cgacaacaag cgctggtccg agcggagcac ggtgtggaag aaaggctggc
     6361 gcaacctgca caccgagggc gggtcgtgca tccgggtccg gctcaacgtc gacggtcacc
     6421 gcaagcccgc gaccgtggaa tgccgctgga cggccggcgc ggacctcggc gaccacacgg
     6481 actcgctcca gttcctcggc gagcagcgca cctccgtcgc cgaactcggc tggagccggc
     6541 acctggagct gtaccggccg ttcctgtcct actccgaact cggcggcctg atcaacggca
     6601 agcccagcga gatgcacgac tccctcgacg gcatcctcgg cctcaagcgg gtggccgaga
     6661 tcgagaagct gctgaaggcc gcccggaagg acaccgacca gcggcgcagg cgggccgccg
     6721 aggaactccc cgcgctgcgt gcactgctca ccgaacaccc cgacccgcgc gcccgccgcg
     6781 ccgaggaggc cctcgccggc cgcaccgccg atctcgacac gctcgacgag gtggccacca
     6841 gcggcgctcc ggacgacgac gcgaccgtcg taccgctgcg tcagctcgcc gtgctcgacc
     6901 tgccgaaccg ggacgacctc gccgcccggg ccgaccggct ccgtgccgcg ctgcgccacg
     6961 tcggcgaact ggccggaacc ccggccgaac aggcacgggc gctcgccggg ctgctccgcg
     7021 ccgcgctggc ccaccacgac aaccaccccg accagccctg cccggtgtgc gggggtcgca
     7081 ccctggacga ggcgtgggcc gtgcaggccc gcacgcaggt acgggagctg accctgcgcg
     7141 cggagcagct cgacgccgcc caccgcgccg gacacgaagc ccggcacgcc ctacgccagg
     7201 ccgtgcccag ccggccgccg gtgctcaccg tcgaccccgg accggacggg atcgacctca
     7261 ccgaactgcg gaaggcatgg cagcgttggc atgacctgac cgccgacacc gacccggcca
     7321 tgctcgtcga actggcaccc accaccttcg acgacctcgc cgccgcgctg gccccggtcc
     7381 gggccgccgc ccgggaagcc cttcaacgcc gccggcagga ctggcagccc gtcgccgacc
     7441 ggatccgtgc ctggatcgac atcgagcgcg ccagccggca ggccgccatc gtgcttcccg
     7501 acctgaggaa ggcgatcgag gcgctgaaga ccatcggtgc gacgatccgc gccgaacgcc
     7561 tccggccgat cgcggccgag gccaccgcca tctggaacac cctgcgccag gacagcaatg
     7621 tcgaactcga cgcgatcagg ctggccggca gcggccccgc ccgccgggtc gacctcgacg
     7681 tacgggtcga cggagtgccc gacgccgccc tcagcgtgat gagtcagggt gaactgcact
     7741 ccctcgcgct ggcgctgttc ctgccccgcg ccaccaccgc ggagagcccc ttccggttcc
     7801 tggtcatcga cgacccggtg cagtccatgg acccggcgaa ggtccacggg ctcgcccagg
     7861 tgctccaccg ggtcgccgcg acccggcagg tcgtcgtctt cacccacgac gaccggctgc
     7921 ccgccgccct gcggcacctg cgcctcgacg cccggatcct caccgtgagc cggcgcgacc
     7981 actcccaggt caccgtcgac accggcaagg accccgccga gcgctacctc aaggacgccc
     8041 agggcatcgc cgacgacgag gacatgtcgg agcagacccg gatcaccgtg gcctgcaacc
     8101 tgatccgcga cgccctggag tacgtctgcc acgagcggat ccggatcgcc ggcctgcgcg
     8161 ccggcacccc cttcgccgac atcgaggaca ccatcgccga ggcgcagtcc gatggcctgc
     8221 ggcccctgct cgcgctggct ctgctcggcg accaccggcg caccgacgag gtggacgtgg
     8281 agctgcgcaa ggtcgactcc gcagcggttc ggctggtgcg ggaggtcaac cggggcgccc
     8341 acggcaacgc cgtaccgaac ctgcacacgc tcgtcgccga cgggcgtcgg gtggcgggaa
     8401 ggttgagccg gccgtgaccc cacaacacct tctcgaccag gccgccgacc tgctggtcaa
     8461 cccccgcccg agcatgcgtc gctgctggca gcgcggcagt gcctgcctca cccgggtcgc
     8521 cctcgaacag gggatgcgcg actactggag caccatcgac gtcaacgtcg ggcgctgccg
     8581 gatgagccgt cagctcctgg ccctgcgggc cttcgccggc gacgagacgg cgctgatcgc
     8641 ccggaccgcc tggcacgggc tctcccgtgc catgcaccac cacacgtacg agttggcccc
     8701 gacgctggcc gagctgcgcg gctggcaccg ggatgtcacg gatctgctgc cccgactcaa
     8761 gccctgagaa tcggctcctg ttgcgcctcg ggacggcatc attgacttct gtcgaagtca
     8821 ttcgccgggt ccgccggttc gtcatcggcg gcccggcgac acgtccccga ggaggaccgt
     8881 gaaactccgc gcccgtctcg ccgccgccct cgccgtgacc ctgaccgccg gcgtcgtcgc
     8941 cgttccgtcc ccggccctgg cctgggcaca gctcaacaac gaccccgact tcatccaggt
     9001 ctacgtcaac aacatcgaga acctcgagac ggtcgacgcg gtgaactgcc ccggcgactg
     9061 ggaggatctc atctactaca tgaagaccta cgagaccagc ccggacctgt tcctcgtgca
     9121 gcagctcgcc gggccgggtg agctgaacac cctcctcgac cgcatgaaca ccctgctggc
     9181 cggggagttc gccggcatcc tcgccgagcc ggacccggag cagatgcagt cgccctgcgg
     9241 cgcggagaag gaccaccaga cgaacgcgat catctggcgg atcggtcggt tcgacatggt
     9301 cgcgggttcg cagctcacct ggcagtcgtt ggccgaggac gccagcggca cctgtgctcc
     9361 caaccaccag gaccgcacca tcaacgtggc cgcccgcctc tacgacaaga tcgcggggag
     9421 gtacgtctcc gccggcagca tccactggcc gaccggcaac agcgacggcc cggcctgtgc
     9481 cccggagaac gccgcccgga cggtggagaa ggtcgagagc atcggcgggg ccctgcgcgt
     9541 ctggggcggc gacgccaacg tctccgacca ggccaacggt gactggcgga gctggtacgc
     9601 caccaccaac ggacaactgg gcgggcggca caactatcgg gacgccgcct tcgacgactg
     9661 ctcggaacgg gtcgacggcg ggacggcgct gcgtcagtgc ctgcgcgacg agcactggac
     9721 cgtcggcggc gacaaccgca tcgacgtcct cttcgcccgt cggggcgacg gcagcatgcc
     9781 catgaccggc gccgagcaca ccgtcacctt cgccgaaggc gaccaggccg acatcgcggt
     9841 gaccggctcc gaccggcagg accgcagcta ctccgaccac cgggcgatcc gggcccgcgt
     9901 ccactactga cctgtcccgc acggccggtc cgaggcgcgc acctcggacc ggcattccgg
     9961 gctccaccgt ccacggtgtc tgccttcggg tgttccgtcg tcacggaacg gggtggatgg
    10021 tggcgtacgc ctgccgcggt gaggcctgga gcagggactt accggagaag atcagttctg
    10081 ccaacggaag ccgctggtga tagaagttca gcgacgagga agcgtcgaag atgtcggccg
    10141 aggccaggaa gaacggaagt tcggcgacga gataccgcat ggcacgttcg atgcgttccg
    10201 tgtcgagcgg cccgtcgtac ccccgggcgg cgaggacgtc cctgctcatc tccgcgcagg
    10261 tctcccacag gatgccgtcc tcgttcaggg tttgttcgca ctccgcgagc ttcctgcggt
    10321 agacctcgtt cgacaccagg tccgacatac ggttcaagcc gtcggtcgcc cgtggtccac
    10381 cactgctttc ccaggctcgt acgacccggt tgcgcaggac gttggtctcg ccgcgcgcct
    10441 tcttgtccgc acggtccggt tcgtagccga gcgcgaggta cgtgtgcttc agggccgagt
    10501 ccgggatgac gacatccacg cgggcgaact cggcacagag ccagcccagg aggtcggtca
    10561 gccgggcgac gttgaagtaa ctgtttccgg ggctgacccc gaggaccgcg tgctgtcgct
    10621 gctcccagat cagacgacag tcgtcggtga agggttcgac gacgaagagc ccttgccgga
    10681 tcggtgtgtt tccgtcctgg ttggtcgcgc cttcggctgt cgtcatcagg tccaccgacc
    10741 ggttcccggg tgcgcggcgc cgttcagata ccgtcggcga agttcctccc ggatgatttt
    10801 ccccgttccg gtgcggggca tgtccttgaa agcaatcacg atcggctcgg cgagctcggg
    10861 cagtttacgt accgcattcc gccactgctc tccgtccagt tggccatcgg gcgtggcgac
    10921 gatcggcacg ggcggtccgt cggacaccgg cagcagcgcc acgtcatgcc ctgccggcag
    10981 attgtcgacg atgacatctt cctgttcgag gcagttcaac cccggcgcgt agttgacctc
    11041 acgatcgatg agcttcaggg cgccggtcct ggtcttgata ccgatgtcac cggtgttcca
    11101 ccactcaccc acggcctttt cgttccagcg ttccggttcc tggtagtacc cggcgcaccg
    11161 tgccctggtg cgtgccaaca ccagtccggg ttgaccgtcc ggcaccggct cgaacgtctt
    11221 cgggtccacg accttgagct ggacgaaccc cggcatcggc cgacccacct tgcggggacc
    11281 cggtcgctgg tcacgccgta tgttcgccgt tcgccgggtg acgaatgtca tgcccatgcc
    11341 gccggtctcg gactgcccgt acccctcccg ccagagcggg aacgggtgcc gggaggcgtt
    11401 caggtacggc cggatcgtcg cccaccgcac cgcgtcgaag ttgttgatga acatgcgtac
    11461 cgaggagaag gcgtcattgc gtgggtcccg ggcgagcccc tccatccgga aatagtcgat
    11521 cggcagcgcc tcgaccaccg tgggtcggtg ctgctggaac aacgggccga cgacctcggg
    11581 atcgctgtcg ctcatcacga gaaccttggt gggggacagg gtcagcaccg agtagatcca
    11641 cgtgaaggcg cgagcgtgga cgtacggcgt gaacatcgcc acggtgtcgt tccgatggaa
    11701 ggtgatcggt ggcaggtgac ggcattccag cctggccatc tggccctgga gcttggccgg
    11761 ggtgtacatg atgagcttcg gaatgccggt ggtgccggag gtgtgggtga gcatcatcag
    11821 ctcgtcgtca cgccgcgcca cgggggacgg gtcggtgctg ccgtagaggt ccgcgaaggc
    11881 cagcgtgtcc ggtacgccct cgtccaggca cagggtccgt tccgcgaacg aggtgagtga
    11941 gcttccgtcg cttcctctgt tttccaggag ttgtctgttg ctgaccagca gccgtggttc
    12001 gacgcgctgg agtagtgctc gcgtcgagtc ggtgggcagc aaaccggaga gcatcaccgg
    12061 aatagcgccg atccggatgc cggcggcggc gagcagaagg cagtcgaagt ggttgtcctt
    12121 gtagacggcg aatcggtcac cgggacggac ccctgccgca ctgagcgctc ctgcggtttt
    12181 ccgcacgcac tgggcgatcg cctctatgtc gaggacggcc tggcctgatg gatcgatatc
    12241 caacgggcgg ttgggacgga agatcattgg cttccccgct tcggccctac ggtcgaagag
    12301 gagacctacg ctggacttcc acaacctaag attgggcacc cgttcccccg tatttacgcg
    12361 gctccgtcag gtgatcacag cgatcaatac cttaggcttc gttaaggtga tccgtcaagc
    12421 cttccgtcga ttcctgatag tgtgcatgag gaaccctgtc gagggcggtt cggtggcgcg
    12481 acatcagcgc ctgggccgtt ccggtttccc gtggccgaat ctccccgctg acctgcgatt
    12541 cgggcagagg gcgcgggtat ttacggcgac gaaaagaagg ggagtcgtgg cggcccgccg
    12601 ggcgtgggga gcccggcggg gccggttcac ccgagtgcgg tccagggctg gttcaggtgc
    12661 gcggtggcca gcacggcgcg gcgggcccga gcctcgtcga gcagccgctg gcgggtctcc
    12721 cgggcggcct ccgggtcggc ctcgtaccgg tagcccacgg cggggtcgac cagctggacg
    12781 gcgtggacca ggacgtcacc ggtgacgatc acctggtcgt ggccgtcctc gacgacgacc
    12841 gactggtggc ccggggtgtg ccccggagtg ggcacgaccc ggacgcagcc gccgcgcccc
    12901 cgggcggagt gcagccgact ggccccgtcc acctcgtgca gctggccggt ggcccgtagc
    12961 ggctcgacca cgtacgtccg ggccgcgtca ccgtcgggca gggtggccac ctcgcggcgc
    13021 tggacgacgt accgggcgtt cgggaacgcc ggcgtgccgt ccaacccgac cgaccagccg
    13081 aagtggtcct cgtgcaggtg ggtcagcacc accgtgtcga cgtccgccgg gtcgatgccg
    13141 gcccggtcca gctcggccgg cagtcggccc ggagtcggcg cccagccctc ggccgggctg
    13201 ccgtccggcc ccaccccggt gtcgacaagc gtcagccgac ctcgcggccc gcgaacggcg
    13261 tagcaccgga agtcgagctg ccaggcgtcc cccgggccga acgcgccggg gtcgacggcc
    13321 cgggccagct cccagtcgtc cgacgtggcc gccgggaacg ccgcctgacg gtcgaggaag
    13381 aacgtcccgg tcgcgtcccg caacgggatc acctcgtacg cgccgaccga ccgcgcgccc
    13441 gacgcccgcg atcccctccc cggcccggtc gcgcccgccg ggacggctgc gcccgccggc
    13501 ccggcgaccg tcacggctgc ggcggcgagc aggaacgtcc gtcgtccgaa ctcggcggaa
    13561 tcgtccatca tggtggcacc tttcgtccgc aggtgccggg tgaggcgacc cacccggcgt
    13621 ctcggcagcg acgctagggc cggtaccggt acgcgggccg ccccgcgacg ggcggtcgac
    13681 ctgatcctgt cgatacctgg ccggctttca ccgccggacc cacccgcacg gcttcttagg
    13741 atggggtgcc gggagtccgc gcccgccggc aggcgggtgg ccgaggtgtg aggtgtggga
    13801 ggccccgatg ccgctcgatg tcgccatgct ggcgtacccc ggcgggggca gcttcgacta
    13861 cgtgaccgtc aacgaggtct tcggcgtgga ctactcccac cggttcggct cctggtaccg
    13921 gacgcgcgcg tgcgggctga cgccggggcc ggtcgagctg gacggcgggg tcatcgtcca
    13981 ggccgggtgc gggctcgacg aggcggagcg ggccgacctg gtggtggtgc cggggtggac
    14041 gagggaacac gaggtcccgc cgcgggagct gctggccacc ctgcggggcg cgtaccggcg
    14101 tggggcccgg atcatgtcga tctgcaccgg cgcgttcgtg ctcgggcacg ccggcctgct
    14161 cgacggccgt cgggtcacca cgcactgggc gatggcaccg ctgctcgcct cgctcttccc
    14221 ggccgcgacc gtggagccgc gcgccctgtt cgtcgacgac cgtcgcgtcc tcaccgccgc
    14281 cggcatgtcg gccgggatgg acctcgcgct gcacgtcgta cgcggggact tcggtgcgga
    14341 cgccgccgcc gcgctcgccc gccggctggt gctggccggc tatcgcagcg gcggccaggc
    14401 gcagttcgtc gacgcgccgc tcggcccgga accggatgat ccgctgccgg acctgctcga
    14461 ctggctgcgg cagcacctgg accagcggtg gtcgttgggc tcgttgggcg cccgggtgca
    14521 catgagcccc cgcaccctgt cccggcggtt ccaccggctc accgggatga gcccgcacca
    14581 gtggctgctg cgggagcgga tcctggcggc gcagcggctg ctggagtccg gggacgagcc
    14641 ggtcgcgcag atcgccgtcc gcaccgggat gggctcggcc ggcaacctgc ggcacagctt
    14701 ccgccgggag ttgggcgtct cgccccaggc gtaccggacg agtttccgtg ccggcggccc
    14761 ccgggcgggc gcgaggagca gcggtccgtg acgaaccgcc gcgcgtgacg tgcgacgtgc
    14821 gcagggcgaa ggtggcgcgg cggccggagc cgccgcgcca ccgttacgga gctggtcggg
    14881 tcaggctgtt cggcagctga ccgtcggcca ggtccagttg ccgttcgcca tgatcgtgat
    14941 tccgaagttg ttgccgttgc cgttcggtgt ggcggtgatg gttccgctgg tgccgctgac
    15001 cgaggcgttc caactgttct ggatccactg gccgccgttc aggttgaggg tgaccaccca
    15061 gttgttggtg ccgctgaccg cgacgttcag gttgaaccgg tcgccccacc gctcgccggc
    15121 ggacagcacc gcggtgcagt tgccgcccgg actggtcggg ccggtggtgg gggtgccgcc
    15181 gccctcccag acggtgatgt cggagctgcc gctgctctgg tagccctcgg tcgccatgat
    15241 ctggtagctg tgggtgccga gattcaggcc ggcgcgggcc caggcgtcga agtggttggc
    15301 ggtggtgatg gtgccgctgc tccgcttctg ctggcgcacg ctccagtact ggtagaacgt
    15361 gcagttgttg ccgtcgatgc agggcgcgtt gacccgctgg ctgcggagga tgtcgtaggt
    15421 gccgccgtcg gtggtgaccg tgcccagccg ctgggcgccg ctgctcgggt tgtagctgcc
    15481 gaagttctcc acgacgtagt actcgatgag cgggttccgc gtccatccgt acagggcgag
    15541 gtaggtgttg ttgttgcccg ggttgtaggt gcccgagtag ctgaccgtcc ggcgggtgcc
    15601 ggtggcccag cccttgccgc cgacccagtt gttggtgctc ctgtcccagc tgctggtgta
    15661 ccggccgttc tcgcgcagcg tcatgctggc gctgccactg tccttccaga acgagaagta
    15721 gtagccgttg tgcgtgccgg tggtgttcga gctgaggtac cggtcggcct cggcgaacgc
    15781 gtggggggcg gccaccaccg tgccggtgac ggcgagcacg gcggcgcaca gggcgccgag
    15841 gagcagccgg agacgcccgc gcctcctcgg tgtgatcgaa ctgtggttca tgtacgtctt
    15901 ccctcctcgt gacagctggc gggaggccag cggcgcggtg cgacccggcc ggctcggtga
    15961 ccaggtacgg gcagccggac actcggctta ccaacaggtg gtggggtgtg ccggtcgtcc
    16021 cgcaggaccg gagggtgggt cgcgggtggt ggcgtcgaca gtgattcagc gctgtcgatg
    16081 gcgacgctat tggctggatc cgtggccagt caacaacttc cggaaatgtt acggaaattt
    16141 gctcgcggcc tcggggttct cgcagggagc gttcgacctg gtcggcagtc ctgtccggga
    16201 acctggtcgg gggcagcggc ccttccgtcg ttaccgaagg agtggagagc cgagccaact
    16261 gatctgaaac tttcggaacc cggatcggtg gtcagggtcg gggcccgacc gtgggcctgt
    16321 cgagatcttg gacagtaacc gtcctgtcag gacggagtcg gtccacgatt gactcgttcg
    16381 ccgggggacg gggtggggac gtcgtcgttg cggaggccga agcgggagcg gagttcggcg
    16441 cggacctcgt cggcctcggg gctgcccacg tcggtgaaga cggccagcgc cagccgcaac
    16501 tgccgtaccc cacggtcgac gtcaccatgt cggcagagca tcaggccgag ccggcgcagt
    16561 gccaccgcct ccccgtgccg gaactcggcc tggcgcatcg ccgccacggc cagctcggcg
    16621 tagcggatcg ccgagtcgac ctggccggcg ccgaggtagg cgtcggccag ggtgcccatc
    16681 atgctgcccc ggttgcgttc gttgaccggc accccggcga cggccaggcc gcgtcgcagg
    16741 tagcgcagcg cggagtggtg gcgaccgagc aggcactgca cctcacccag gttgcccaac
    16801 gcgttggcct cgatctccgg ggcgttgctg cggcgggcca ggcggaccgc cgtacggcag
    16861 aaccggatcg actcctcgta gcgtccggcg agccggtacg ccatgccgat gttgccggtc
    16921 gtcgcggcgc aggagcgcag gtcgccgagt cgtcgacgca gccgcaggct ccgcttgaag
    16981 caggcggccg actcgtcgta cgcgccggcc atgccgtgca ggacgccgag ctggttgagc
    17041 gcgcgggcct gcccctcgtc gtcgccggcc gcgtcgctga ggtccaggct gcgccggagc
    17101 aggtgcgtcg cctcgtctgt gcggttgcgg tggaagtggg ccaccgccag gtcgtgcagc
    17161 agcctggcct gcgccaccgg gtcgccgagc cgctcggcgg cggccacccc ggcccggtcc
    17221 acggtgatcc agtcgtccac gtggccacgc atctcgaaga agaggaagag actgtcggcc
    17281 aggtcgacgc cggcccgcgc ggtggcctcc gcccgggcgg cctggacgac ccccgccacc
    17341 aggttcggtc gctcggcgga caaccaggcc agggccgccg gggcgtcgtc gaaggcggac
    17401 gggccggcga ccgtcggcac ccgggtgccc ggacgcagca ggcccaacgc ctggcgggtc
    17461 cgctccaggt ggtgggcgag catccggtgc accgccgccg tcggtaccgg ctccggctcc
    17521 tcggcggccc gttcgcgggc gaagagccgg atcaggtcgt gcatggcgta ccgtccggcg
    17581 gtggtctcgt cgagcaggcg cgcgtcgaca agttcgtcga gcgcgtcgag cgccgggggc
    17641 aggtcgacgt cgagcagcgc ggcggccacc tcgccggtga cgtccacgct gtcgacggca
    17701 ccgacgagcc ggaacagccg gggcgccagc ggatgcgtca ggtgctggta gctgaccagg
    17761 aagctggtgc gtaccgcgat gtcgccctgg cgcagctcgt gcagccggcg gtgctcgtcg
    17821 cgcaggcggt cgtcgagcgt cgcgaggctc caggccggtc gggcggccag ccgggccccg
    17881 gcgatccgga cggccagggg caacccgccg aggcggcgga ccagccgggc cgcggtgtcg
    17941 gcctcggcgt ccacccgcgc gtcaccggcg agccggcgga gcaggtcgac ggcgtcggcc
    18001 tcggcgagga tgtcgagttc caggtgggtg gccccgtcca cgtcggtgag cttccgccgg
    18061 gaggtcacca ggaccgcgca gccgtcaccg gccgggatca acggccgtac ctggccggcg
    18121 tcgtgcgcgt tgtcgagcac cagcagcacc cgccgcccgg ccagcaggga acgcagtgcg
    18181 gccgacgcct cggccacgtc ggtggggatc gccgtttcgg ccaccccgag cacccggagg
    18241 aaccggccca gcgcgctgtg cggcggcagc gggcacacgc cggccgtcgc gccccgcagg
    18301 tcgacgtaga gctgcccgtc cgggaaggcg gtggccaccc ggtgcgccgc ctgcacggcc
    18361 agcgccgact tgccgacccc ggccgggccg gtgaccacgt acaacggcat ccggtggcgc
    18421 ggcccggtgg tcagcgcggc ggtcagcgcg gcgaggggcc ggtcgcgtcc gacgagcacc
    18481 ggggcggtgt ggggtagttc ccggacggca gtcgcggggg gccgggccgg gacggcggcg
    18541 ggcggtgtcg gctggcgggc cagctcggcg agccggcgca gcaccggacc gggttccatc
    18601 gtgagttcgt cggccagcgc ccgccggtaa cgccggtacg cgtcgacggc ctcgctgcgc
    18661 cgaccgaggt ggtgcagcgt catgacctgc tgttcggcga agcgttcgtg cagcgggtac
    18721 cgggcgacga gttggtccag caccgccagc atctcgtcgt gccgccccag gtcgagttcg
    18781 acgtcgacgt actcctcgta cgccgacagc cgccgccggt ccagccaggc gacgctgcca
    18841 ccgaagccgc ccagcccggc cacgtcggcc aggggagcgc cccgccacag gtcgagcgcg
    18901 gaccggtaga gcgtggcggc ccgtccggtc ggaccggcgc ggaccgccgc gcgggcggac
    18961 tccagcagga tgtcgaaccg ggacacgtcg aggtcgtccg ggtcgacggt gatgcggtag
    19021 ccgggcgggt cggacgccaa ccggtcaccc accagctgcc gcagccgcca cacgtggagc
    19081 tggacgttct tgcgggcggt gcgcggcggc tcgtcgcccc agagcaggtc gacgatgtcg
    19141 tcgaccgggg tgatccggtt gacgtgcagg agcagggcgg cgagcaccgc ccgctgtcgg
    19201 ggcggcccgg ccggaaccgg ctggccgtcc gaccggatct gcaccggtcc gagcagcccg
    19261 aaggtcagtc gaccagccat gtcatcgcag cgtaccgggg cgcatatacc aaagatagat
    19321 cgacggttgg gaatcttcgc ggcactcgat cagggaaagg agatctcccg tgtccgatcg
    19381 aaccaccggg gaacggctgc cggcctgggt gcccgcgatg tgggccgccg tcctcttcgc
    19441 cgcgctgctg acacccgccg gcccggccca cgcctcccac ccgaacccct cctccacccg
    19501 ccacctgcac aacatgcact acgcctcgtc cggcacctcc gcgggcgcca acgacgagca
    19561 gttctgcgtc gagtccaccg acacggccaa ggtgagccac agcagtgccc gcaccttcgt
    19621 caagcagacc ctgaccgaga tgggcgccgg gaaggtctgg gacggcacgg gcaactggcg
    19681 catcgacctg tgggcgacca acagcaactg caccgcgtac ccggcctcga cccggagcac
    19741 gatcgagatc gaggtccact acggctggga ctggagccgc ttctgcggcg cgcccaccgg
    19801 ctactacaac tgcgtcgtac acgacaaccc ggtgtgggac gcggcccacg gccactacga
    19861 cagccagtgg gcctacgtct acctggtctt cagctccggc ggacgcctgg acaacgtggg
    19921 gcgggccttc atcaaccacg agttcgggca cgtcttcggc ctcgccgacg acaacggcgt
    19981 ctgcaacccg ccgagcctca tgcacagctc gatcgtcggc tacagctgca ccaactggcc
    20041 ctactggtac cccagcccgt ccgacttcca gtccgtcgtc aacgtgatgg cctgacggtc
    20101 cgtagaagga ggcctgagat ggcacgacgt tccgtgcgta cccgcctggt gaccacgctg
    20161 acggcctgcc tcgcgtcggc gttggccgcc ggagtgatcg tgacggccgt gctcgaccgc
    20221 tccggcggcc acgaccacgc cgaccacgcc catctcgaca ccgccagcgg actgctcgtc
    20281 accagccggt cggccctgcg gatgtgcgtc gaggccggca ccggcaccgg gcaggatgcc
    20341 atccgggccg acctgctcgc gggcctggac cgggcccgcg agcaccccaa ctgggccgcc
    20401 agctacggca gggtccgcct caccccggag accgcgctgg acttcggctg cccggccgcc
    20461 cgcctgccgg accggatcga ccgtacggcg ctcgccgggc ccggcatcac cgacgacccg
    20521 agcgcgtacc gcgtctgggt ctacgtcctc gacgaaccga ccgccgaccg gatcctcggc
    20581 gtcggccagc cggccgacgt cgcgaccgcc gaactcatgc gggaggggca gagcctctgg
    20641 ccggtctcca cggcgctgct gatccggcag agccggttgg gtgacccggc caccgtgggg
    20701 cggagcctgc ggaccgcgtt gggcct
//
