LOCUS       NZ_AP023438            14870 bp    DNA     circular CON 15-DEC-2024
DEFINITION  Micromonospora sagamiensis strain JCM 3310 chromosome, complete
            genome.
ACCESSION   NZ_AP023438
VERSION     NZ_AP023438.1
KEYWORDS    RefSeq.
SOURCE      Micromonospora sagamiensis
  ORGANISM  Micromonospora sagamiensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Micromonosporales; Micromonosporaceae; Micromonospora.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 6930736)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu
            Genome Coverage                   :: 62X
            Sequencing Technology :: Illumina HiSeq X Ten; PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014680085.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 15:12:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,941
            CDSs (total)                      :: 5,859
            Genes (coding)                    :: 5,751
            CDSs (with protein)               :: 5,751
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 30 of 108
            Pseudo Genes (incomplete)         :: 88 of 108
            Pseudo Genes (internal stop)      :: 5 of 108
            Pseudo Genes (multiple problems)  :: 15 of 108
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:39:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3937973
            Orig. end                         :: 3952843
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP023438.1.
            Genome sequencing and analysis were conducted under the Global
            Catalogue of Microorganisms (GCM) 10K type strain sequencing
            project (http://gctype.wdcm.org). The genomic data are also
            available at http://gctype.wdcm.org/sequencing/GCM10017556
            Annotated by DFAST v.1.2.7 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..14870
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="NAGGN"
                     /region_number="20"
                     /rules="((TIGR03104 or cds(GATase_7 and Asn_synthase)) and
                     TIGR03103 and (TIGR03106 or Peptidase_M42))"
                     /tool="antismash"
     cand_cluster    1..14870
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((TIGR03104 or cds(GATase_7 and
                     Asn_synthase)) and TIGR03103 and (TIGR03106 or
                     Peptidase_M42))"
                     /kind="single"
                     /product="NAGGN"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..14870
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:9870](-)"
                     /cutoff="10000"
                     /detection_rule="((TIGR03104 or cds(GATase_7 and
                     Asn_synthase)) and TIGR03103 and (TIGR03106 or
                     Peptidase_M42))"
                     /neighbourhood="5000"
                     /product="NAGGN"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..9870)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="((TIGR03104 or cds(GATase_7 and
                     Asn_synthase)) and TIGR03103 and (TIGR03106 or
                     Peptidase_M42))"
                     /neighbourhood="5000"
                     /product="NAGGN"
                     /protocluster_number="1"
     gene            118..474
                     /locus_tag="IGA62_RS17420"
                     /old_locus_tag="GCM10017556_34710"
     CDS             118..474
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074683.1"
                     /locus_tag="IGA62_RS17420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34710"
                     /product="YkvA family protein"
                     /protein_id="WP_145819879.1"
                     /transl_table=11
                     /translation="MARTLKRSAAFAALARALMAGARGGPSLGARLAALPRMIKATARG
                     EYDGGLRLALMTAAAAYVVSPVDLVPEMFLAVLGLVDDAVMVTWLAGSVLAETERFLEW
                     EARRASVVPGSVLS"
     gene            549..1919
                     /locus_tag="IGA62_RS17425"
                     /old_locus_tag="GCM10017556_34720"
     CDS             549..1919
                     /EC_number="4.2.1.22"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742048.1"
                     /locus_tag="IGA62_RS17425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34720"
                     /product="cystathionine beta-synthase"
                     /protein_id="WP_145819877.1"
                     /sec_met_domain="PALP (E-value: 5.7e-71, bitscore: 237.7,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQYYDNVVELIGNTPLVKLRNVTAGIQATVLAKVEYLNPGGSVKD
                     RIAMRMVEDAEAAGILKPGGTIVEPTSGNTGVGLALVAQLKGYRCVFVCPDKVSQDKQD
                     VLRAYGAEVVVCPTAVAPEDPRSYYNVSDRLAREIPGAWKPNQYSNPANPRSHYETTGP
                     ELWKQTGGGLTHFVAGVGTGGTISGIGRYLKEASEGRVKVIGADPEGSVYSGGTGRPYL
                     VEGVGEDFWPETYDKSVADQIIEVSDKASFEMTRRLAREEGLLVGGSCGMAVVAALEVA
                     RDAGPDDVVVVLLPDSGKGYLSKIFNDAWMARYGFLTASGDEPTVADALAAKPSRLPEL
                     VHVHPTETVRDAIDYMREYGVSQLPVLKAEPPVVTGEVAGSIAEKDLLDALFTGQAHLH
                     DTIERHMGEPLPMVGGGQPVSEAVALLEKSDAALVLIDGKPKGVLTRQDLLAHLGAR"
     gene            complement(2064..>2297)
                     /locus_tag="IGA62_RS17430"
                     /pseudo=""
     CDS             complement(2064..>2297)
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184427.1"
                     /locus_tag="IGA62_RS17430"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="HIT family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="PVHIVVAPKRHVPSLIDLGGATEDLLHRVLEVVRHAAARVEREQG
                     ACRVPTNLGRYQHSEHLHFHVCSGQPSRPDPA"
     misc_feature    complement(2371..2384)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.28"
                     /tool="antismash"
     misc_feature    complement(2421..2434)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.1"
                     /tool="antismash"
     misc_feature    2459..2473
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     gene            2462..2746
                     /locus_tag="IGA62_RS17435"
                     /old_locus_tag="GCM10017556_34730"
     CDS             2462..2746
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018725755.1"
                     /locus_tag="IGA62_RS17435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34730"
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_145819873.1"
                     /transl_table=11
                     /translation="MRIVPYSEVRQNLAKMLDHVVDDAEEIVVTRSGREAAVIISLREY
                     ESLKETAYLMASPANARRLNEAVEELRNGGGEMHDLIDPNAGSGEASAA"
     gene            2743..2997
                     /locus_tag="IGA62_RS17440"
                     /old_locus_tag="GCM10017556_34740"
     CDS             2743..2997
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018829754.1"
                     /locus_tag="IGA62_RS17440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34740"
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_145819871.1"
                     /transl_table=11
                     /translation="MKVQFASRGWEDYLTWQRDRQMLKRVNALIEDIRRNGHQGIGKPE
                     QLRGNWSGFWSRRIDQEHRLVYRIVDDTVQIAQCRYHYE"
     gene            complement(3235..3753)
                     /locus_tag="IGA62_RS17445"
                     /old_locus_tag="GCM10017556_34750"
     CDS             complement(3235..3753)
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742049.1"
                     /locus_tag="IGA62_RS17445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34750"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_145819869.1"
                     /transl_table=11
                     /translation="MTLTLRHAVADDLMAVGSLHQRSRVAAYSPFLPPEALAAPTPEGM
                     GLYWVERWSYERDDHLMTVAERDGRLVGFTYVGPDELDGVVDPQAAMLRAIHLEPTEQG
                     RGTGRTLMRDALRTMRSWERSRATLWVLRDNAHARRFYERGGWAPDGRERDERFGPVVT
                     AQVRYARPL"
     gene            complement(3750..4682)
                     /locus_tag="IGA62_RS17450"
                     /old_locus_tag="GCM10017556_34760"
     CDS             complement(3750..4682)
                     /EC_number="3.1.26.11"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018586560.1"
                     /locus_tag="IGA62_RS17450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34760"
                     /product="ribonuclease Z"
                     /protein_id="WP_145822163.1"
                     /transl_table=11
                     /translation="MSMRELTVLGTASQTPTRQRNHNGYVLRWDDEVILFDPGEGSQRQ
                     LLHTTVTATDLTRICVTHFHGDHSLGLPGIIQRLSLDRVPHPVAVHFPAGGAEYFARLR
                     YATSFHETAELACEPVAADGQRIALRGGHTLTARRLRHPIETYGYQLVEPDGCRMLPER
                     LAAYGIGGPAVGELIRVGHLDVGGRRVHRDEVAVPRPGQRFAFVMDTGLCDAVYALAEH
                     ADLLVIESTFLDSEAALAAEVGHLTAAQAARVATESGVRRLVLTHFSQRYADPARFAAE
                     AREHFTGDLVIAEDLATVAVPPRRVASPG"
     gene            complement(5001..6206)
                     /locus_tag="IGA62_RS17455"
                     /old_locus_tag="GCM10017556_34770"
     CDS             complement(5001..6206)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     TIGR03106"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     Peptidase_M42"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904826.1"
                     /locus_tag="IGA62_RS17455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34770"
                     /product="osmoprotectant NAGGN system M42 family peptidase"
                     /protein_id="WP_145819867.1"
                     /sec_met_domain="TIGR03106 (E-value: 4.3e-145, bitscore:
                     481.3, seeds: 7, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_M42 (E-value: 9.1e-55, bitscore:
                     184.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTERRPAPLPLDLDYLRQVLLELLEIPSPSGRTDHVQQYVGERLS
                     ALGITSTLTRRGALSACLPGPRSTGADRAIVVHTDTIGGMVKRLKENGRLELKSIGTHS
                     ARFAEGAHVRIFTDDLDRVITGQVLPLKASGHRYNEAVDLQGVGWEQVEVRVDEPVEDI
                     AGLRALGIDAGDFVAFLPNPTITPSGFVKSRHLDDKAGVAAVLTALKAMVDAGVQPRVS
                     AHLLVTVTEEIGHGASHGLDPDVAEIVSVDAAVVAPGQQSRETAATLAMGDGVGPFDYH
                     LTRNLASIAAEHGVDLVRDVFDYYRSDVAAAVEAGAHARVALLGFGVDATHGHERTHLD
                     GLRHLTQLLCLYLQSDLVFPEWDAEPEGDLADFPSLAVQPASEEGPREGPIGIADPAPH
                     PS"
     gene            complement(6203..8083)
                     /gene="ngg"
                     /locus_tag="IGA62_RS17460"
                     /old_locus_tag="GCM10017556_34780"
     CDS             complement(6203..8083)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ngg"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     TIGR03103"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018810760.1"
                     /locus_tag="IGA62_RS17460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34780"
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="WP_145819865.1"
                     /sec_met_domain="TIGR03103 (E-value: 2.5e-233, bitscore:
                     773.9, seeds: 7, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 6.8e-06, bitscore: 24.2,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 5.7e-06,
                     bitscore: 24.4, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTETLATGAALTEGQRARMARRRERVGPGGDPVAPGEPAGRSTDP
                     DAVPPAGRTDVVLDCGWGRLVFGQTFRDPADVADVLRSEAAGSRDICIYLRDPHVLVSR
                     LHDELFIDPSLTYRLPLSPDAAPGPGPIGGEPAADGGADLPGLRVRQLRDAADADAVNR
                     IYASNGMVTAPVDVLVANAATPSFLHLVAESATGEIVGTITGVDHVEVFDDPDNGASLW
                     CLTVDFNHAPPGTGQALLTELAARLTDRGRAFVDLSVLAENDGAIRLYERLGFYRTATL
                     CVKRKNPINERLFLPSLPAGYDELNPYARIVADEAMRRGIRVEVTDPAWGELRLTSGGR
                     TILTRESLSELTSAVAMSRCDDKRVTRRILTEAGLSVPRGRTATGDDADEAFLAEIGAV
                     VVKPARGEQGNGITVGVRAPEALRTAVELARRYCPDVLIEELRAGADLRVVVIDHEVVA
                     AAVRQPASITGDGVHDVTELIERQSRRRAAATGGESRIPIDDMTREVVAEAGYGMHDVL
                     PEGRMIAVRRTANLHTGGTIHDVTGDLHPEIAEACVAATRALDIPVTGLDLLVTAPDQP
                     EHVFIEANERPGLANHEPQPTAERFVDLLFPGTRAPHRLWSPAGTASSTA"
     gene            complement(8086..9870)
                     /locus_tag="IGA62_RS17465"
                     /old_locus_tag="GCM10017556_34790"
     CDS             complement(8086..9870)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     GATase_7"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     TIGR03104"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904828.1"
                     /locus_tag="IGA62_RS17465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34790"
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="WP_145819863.1"
                     /sec_met_domain="TIGR03104 (E-value: 1.4e-289, bitscore:
                     959.7, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="GATase_7 (E-value: 1.4e-41, bitscore:
                     139.6, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 2.5e-85, bitscore:
                     285.6, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCGLAGELRRDGSRADVTAVERMAATMHDRGPDDSGVWSQGPVAL
                     GHRRLKIIDLSAASGQPLVDSAAGLTGVFNGCVYNYRELRAELQAKGHVFFSSGDTEVV
                     LKAYAEWGDDFVDHLVGMFAVAITERATGRLVLARDRLGIKPLYLAETPDRVRFASTLP
                     ALLAGGGIDTTVDPVALAHYLSFHSIVPPPRTILRGVAKLPPATVRVYEPDGRVTERVY
                     WDPSFTRHPDRAGWSERDWQDALHESLTTAVRRRMVADVPVGVLLSGGLDSSLVVALLA
                     EQGQRGLATFSIGFEAVGGREGDEFRYSDLIAKTFDTDHHQIRVATNDLVPPLEAAVAA
                     MSEPMVSHDCVAFYLLSETVARHVKVVQSGQGADEILGGYHWYPPLAGVDRAAALDTYA
                     KAFFDRDAAGLARVVNPDRLADGDPAREFVAAHLGRVGADTAVDAGLRIDTQIMLTDDP
                     VKRVDNMTMAHGLEARVPFLDHEFVELAASCPPELKLAQGGKGVLKEIGRRVLPWEVID
                     RPKGYFPVPGLTHLEGKVLDRVRDALHAPEARRRDLFRADYVDALLTDPNAELTPLNGN
                     KLWQLGLLEMWLQSHGIA"
     misc_feature    9902..9919
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 21.71"
                     /tool="antismash"
     misc_feature    complement(10006..10021)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     misc_feature    complement(10033..10048)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.29"
                     /tool="antismash"
     gene            complement(10179..10841)
                     /gene="msrA"
                     /locus_tag="IGA62_RS17470"
                     /old_locus_tag="GCM10017556_34800"
     CDS             complement(10179..10841)
                     /EC_number="1.8.4.11"
                     /GO_function="GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_function="GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215983.1"
                     /locus_tag="IGA62_RS17470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34800"
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="WP_145819861.1"
                     /transl_table=11
                     /translation="MFLRRMKAEMITPDQALPGRPLAMPVSDRHEVLGTPLKGPFPEGA
                     QVAVFGMGCFWGAERLFWGLPGVITTSVGYAGGYTPNPTYEEVCSGRTGHAEVVQVVYD
                     PTTIAYEDLLKVFWENHDPTQGMRQGNDVGTQYRSTIYTTSDEQAATAKASRDAFAPIV
                     ARAGRGEITTEIDQLGDYFFAEDYHQQYLAPTKNPNGYCNHGPNGMSCPVGVARTSG"
     gene            11134..11709
                     /locus_tag="IGA62_RS17475"
                     /old_locus_tag="GCM10017556_34810"
     CDS             11134..11709
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.5"
                     /locus_tag="IGA62_RS17475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34810"
                     /product="N-acetyltransferase"
                     /protein_id="WP_186500037.1"
                     /transl_table=11
                     /translation="MEIREMEPGTDEAVARSLLRVQRAAYAVEAVLIGDDRIPTLRETL
                     DELRAAPLRWLVAFADDAATEDEVADRVATDHTAADDAAAGGSGGGRLVGAVAWTEDAA
                     TVDIDRLVVDPAVHRRGVGRMLVGEVLTRAGGRTVVVATGRANRPARALYESFGFVVLG
                     DAEPVPGLWITRYTRPGPEERAAATRSA"
     gene            complement(11658..12089)
                     /locus_tag="IGA62_RS17480"
                     /old_locus_tag="GCM10017556_34820"
     CDS             complement(11658..12089)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904830.1"
                     /locus_tag="IGA62_RS17480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34820"
                     /product="HIT family protein"
                     /protein_id="WP_145819857.1"
                     /transl_table=11
                     /translation="MSGCVFCGIVAGTVPAFRVTDEADGVAFLDTRPVFRGHVLVAPRA
                     HLVTLADLPAESIAGYFGLVRRLAVAVEAGLGADGTFVAMNNRVSQSVPHLHTHVVPRT
                     KGDGLRGFFWPRTRYTDDAEAQSYADRVAAALSSGPGRV"
     gene            12182..13324
                     /locus_tag="IGA62_RS17485"
                     /old_locus_tag="GCM10017556_34830"
     CDS             12182..13324
                     /EC_number="2.5.1.48"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0019346 - transsulfuration [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (49-186). E-value:
                     1.1e-08. Score: 26.5. Matches aSDomain:
                     nrpspksdomains_IGA62_RS17485_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1168: O-succinylhomoserine sulfhydrylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904831.1"
                     /locus_tag="IGA62_RS17485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34830"
                     /product="cystathionine gamma-synthase"
                     /protein_id="WP_145819855.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.1e-06, bitscore:
                     26.5, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 5.3e-07,
                     bitscore: 27.6, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNHGFETLAIHAGQDPEARTGAVIPPIYQTSTYAQDAVGAPRLGY
                     EYSRSGNPTRDALQECLAALEGGPVGLAFASGLAAEDTVLRTVCKPGDHVVIPDDAYGG
                     TYRLFAKVAERWGLAYTPAKVSDPDAVRAAIRPGSTKIVWVETPTNPLLGIADIAALAG
                     VAHDAGALLVVDNTFASPYLQQPIAHGADVVVHSTTKYVGGHSDVVGGALVAADRELGE
                     QLRYHQNAMGAVNGPFDAWLTLRGVKTLGVRMDRHCDNAERIAAYLDGHAKVAEVIYPG
                     LPSHPGHEVAAKQMRRFGGMISFRATGGEEHAVEICNRARLFVLAESLGGVESLIEHPG
                     RMTHASAAGSPLEVPGDLVRLSVGIETVDDLLADLEQALG"
     misc_feature    complement(12192..12210)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.13"
                     /tool="antismash"
     aSDomain        12329..12739
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IGA62_RS17485_Aminotran_1_2.1"
                     /evalue="1.10E-08"
                     /label="IGA62_RS17485_Aminotran_1_2.1"
                     /locus_tag="IGA62_RS17485"
                     /protein_end="186"
                     /protein_start="49"
                     /score="26.5"
                     /tool="antismash"
                     /translation="SGNPTRDALQECLAALEGGPVGLAFASGLAAEDTVLRTVCKPGDH
                     VVIPDDAYGGTYRLFAKVAERWGLAYTPAKVSDPDAVRAAIRPGSTKIVWVETPTNPLL
                     GIADIAALAGVAHDAGALLVVDNTFASPYLQQP"
     gene            13341..14750
                     /locus_tag="IGA62_RS17490"
                     /old_locus_tag="GCM10017556_34840"
     CDS             13341..14750
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1105: amidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904832.1"
                     /locus_tag="IGA62_RS17490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017556_34840"
                     /product="amidase"
                     /protein_id="WP_145819853.1"
                     /transl_table=11
                     /translation="MAVQDIMPTWVGATAKQIARGVRRGDVSATQVVADHLDHIARADA
                     DLAAFRTVRGGEAITEAEKVDEQEDLANLPLAGVPVAVKENTPVAGLPTWRGSAAVRTP
                     VAESDHEVVRRLRGAGAVILGVTRMPELGLWAVTDDETAVTRNPWDPGRTPGGSSGGAA
                     AAVAAGLVPIAHANDGLGSIRIPAACCGLVGLKPGRGVVPWQLGAEDWFGLAEHGMLTT
                     TVADAAVGFQVLAGRPQEKLVPPQRLRVGVSLRSPVRGIAADHPNRDAVAAAGRLLAAA
                     GHDTVPADPVYPTALGLKGVATWLAAAAVDVRAGGLERRHLQRRSRRHVALGEWAQRRG
                     YVRQADRDAWRERSIGFFTDHSVDLLLTPALASAPPAAQTWSGRSWRSNMSTSIRYAPY
                     AAPWNIAGLPALVVPVGRRPDGLPVAVQLVGPPGSELLLLGVAGQFEMQAPWARHAPGY
                     PRVGTGSPAAA"
ORIGIN
        1 gcctggcagc gggacattct gggtacgtca tccatcgacc gccggccgtc cgggccggtc
       61 gtcgcggcgg cggttgcgcc ggcccggcac gctggtcgag gagagagggg cactgacatg
      121 gcgaggacgt tgaagcggag cgcggcgttc gccgcgctgg cccgggcgct gatggcgggg
      181 gcgcggggtg gcccgtccct gggtgcccgg ctggcggcgc tgccccggat gatcaaggcc
      241 accgcgcgtg gcgagtacga cggcggcctc cggctggcgc tgatgacggc ggcggcggcg
      301 tacgtcgtct ccccggtcga cctggtaccg gagatgttcc tggccgtcct cggcctggtc
      361 gacgacgcgg tgatggtcac ctggctggcc ggcagcgtgc tggccgagac ggagcggttc
      421 ctggagtggg aggcccgccg cgcgtccgtg gtccctggga gtgtcctgtc ctgacgccac
      481 gtaccctggg cgtcgaccaa ccgtcgcccg gcgccgctgc cggcaccgga ggagaagggc
      541 acaacgaggt gcagtactac gacaacgtcg tcgagctgat cggcaacact ccgctggtga
      601 agctccgcaa cgtcaccgcc ggcatccagg ccaccgtgtt ggcgaaggtg gagtacctca
      661 accccggcgg gtcggtgaag gaccggatcg cgatgcggat ggtggaggat gccgaggccg
      721 ccgggatcct gaagccgggt ggcaccatcg tcgagccgac cagcggcaac accggcgtcg
      781 ggctggccct cgtggcgcag ctcaagggct accggtgcgt cttcgtctgc ccggacaagg
      841 tcagccagga caagcaggac gtgctgcggg cgtacggggc cgaggtggtg gtctgcccga
      901 cggcggtcgc cccggaggac ccccgctcct actacaacgt ctccgaccgg ctggcccggg
      961 agatccccgg cgcgtggaag cccaaccagt acagcaaccc ggccaacccg cgttcgcact
     1021 acgagaccac cggcccggag ctgtggaagc agaccggcgg cgggctgacc cacttcgtgg
     1081 ccggcgtcgg caccggcggc accatctccg gtatcgggcg ctacctgaag gaggcgtccg
     1141 aggggcgggt gaaggtgatc ggcgcggacc cggagggctc ggtctactcc ggcggcaccg
     1201 gccggccgta cctggtcgag ggggtcggtg aggacttctg gccggagacg tacgacaagt
     1261 ccgtcgccga ccagatcatc gaggtctccg acaaggcgtc cttcgagatg acccggcggc
     1321 tggcccggga ggaggggctg ctggtcggcg gctcctgcgg tatggccgtg gtggccgccc
     1381 tggaggtggc ccgcgacgcc ggcccggacg acgtggtcgt ggtgctcctg ccggacagcg
     1441 gcaagggtta cctgtcgaag atcttcaacg acgcctggat ggcccggtac ggcttcctga
     1501 cggccagcgg tgacgagccg acggtcgccg acgcgctcgc cgccaagccg agcaggctcc
     1561 ccgagctggt gcacgtgcac cccaccgaga cggtccggga cgcgatcgac tacatgcgcg
     1621 agtacggcgt ctcccagctg ccggtgctca aggccgagcc gccggtggtg accggcgagg
     1681 tggccggttc gatcgccgag aaggacctgc tcgacgcgct cttcaccggc caggcccacc
     1741 tgcacgacac catcgagcgg cacatgggtg agccgctgcc gatggtcggc ggcgggcagc
     1801 cggtcagcga ggcggtggcc ctgctggaga agtccgacgc cgcgctggtg ctgatcgacg
     1861 gcaagcccaa gggcgtgctc acccggcagg acctgctggc ccacctcggc gcccgctgag
     1921 catggcaacc ggcctgtccc agggcaggcc ggttgccacc cagttcaggc cgtgctgcgt
     1981 ctccgggcac gaaaccgacg ttgagacgca cgtccaaagc cgctgccaag cgctctggat
     2041 gcagaccgct cgtggcccac gctctatgca ggatcgggcc gggagggctg cccgctacag
     2101 acgtggaagt gcagatgttc ggagtgttgg tatcggccga ggttggtcgg cactcggcag
     2161 gcaccctgct cccgctcgac gcgggcggcg gcgtgacgga ccacctctag aacgcgatgc
     2221 aagagatcct cagtggcacc gccgaggtca atgagagatg gcacatgacg cttcggcgcc
     2281 acgacgatgt gcaccggcgc ggcatcacca ggcgaacccc gcgaccatca ccggcgagcc
     2341 actcccccta cacccgcttc gctccagcgc ttgatcggca cccaaggcca gacgtggggg
     2401 ctcacctgcc agggaaccta ttgggttgta caatttcttg tacgtcacgt tgggaggcat
     2461 catgcgcatc gttccctact cagaagtccg gcagaacctg gcaaagatgc ttgaccatgt
     2521 cgtggacgat gccgaggaaa tcgtggtcac ccgctccggc cgtgaggccg ccgtcatcat
     2581 ctcgctacgt gagtacgagt cgctgaagga gacggcatac ctgatggcca gccccgccaa
     2641 cgcgcgccgt ctgaacgaag ccgtcgaaga gttgcgcaac gggggtgggg agatgcacga
     2701 cctgatcgat cccaacgccg gctcgggcga ggcgtcggca gcgtgaaggt ccagttcgcc
     2761 agccggggct gggaggacta tctgacctgg caacgggatc gccagatgct caagcgggtg
     2821 aacgccctga tcgaggacat tcgacgcaac ggccaccagg gcatcggcaa accggagcaa
     2881 ctccgcggca actggtcggg attctggtcg agacgaatcg accaggaaca ccgcctggtg
     2941 taccggatcg tcgatgacac ggtgcagata gcgcagtgcc gctatcacta cgaatgagca
     3001 cgaatcagcc ggcccggcca gggctcacgt cacccgagcg cgcaagggac gcctgatgcc
     3061 caccctcacc gccggtcgac ctgtcgggtg gccctggtgt cgacacgcca cggtgccggt
     3121 acggttcgcg caatggtcca tcagccatct agaaaccgca ggtcattgca ctggtggggc
     3181 gtgaacccgc agccttggga ttagaagtcc ctgctccggg ctcccaccgc acagtcagag
     3241 ggggcgggcg tagcggactt gggcggtgac gacggggccg aatcgctcgt cgcgttcccg
     3301 gccgtccggc gcccagccgc cgcgctcgta gaagcggcgg gcgtgcgcgt tgtcccggag
     3361 cacccagagg gtggcccggc tccgctccca cgaccgcatc gtgcgcagag cgtcgcgcat
     3421 cagcgtccga ccggtgcccc ggccctgttc ggtgggctcc aggtggatgg cccggagcat
     3481 cgccgcctgc gggtcgacca cgccgtccag ctcgtccggg ccgacgtagg tgaagccgac
     3541 cagccggccg tcccgctcgg cgacggtcat caggtggtcg tcccgctcgt acgaccagcg
     3601 ctccacccag tagagcccca ttccctccgg cgtcggggcg gccagcgcct ccggcggcag
     3661 gaacggggag taggcggcga cccgggaacg ctggtgcagg ctgccgaccg ccatcaggtc
     3721 gtcggcgaca gcgtggcgga gagtgagcgt caccccggcg aggctacccg ccggggcggc
     3781 accgcgaccg tcgccaggtc ctcggcgatc accaggtccc cggtgaagtg ctcgcgggcc
     3841 tcggcggcga accgggccgg gtcggcgtac cgctgggaga agtgggtgag cacgagccgg
     3901 cgtacccccg actcggtcgc gacccgcgcg gcctgggcgg cggtgaggtg cccgacctcg
     3961 gcggcgagcg cggcctccga gtcgaggaag gtcgactcga tcaccagcag gtcggcgtgc
     4021 tcggcgagcg cgtacaccgc gtcgcagagc ccggtgtcca tcacgaaggc gaaccgctgg
     4081 ccgggccggg gcacggcgac ctcgtcgcgg tgcacgcggc gtccgccgac gtccaggtgg
     4141 ccgacgcgga tcagctcccc gacggccggg cccccgatgc cgtacgcggc cagccgctcc
     4201 gggagcatcc ggcagccgtc cggttcgacc agctggtagc cgtacgtctc gatcgggtgg
     4261 cgcagccggc gggcggtcag ggtgtggcca ccgcgcagcg cgatccgctg cccgtcggcg
     4321 gcgaccggct cgcaggccag ctcggcggtc tcgtggaagc tggtggcgta ccggagccgg
     4381 gcgaagtact ccgcgccgcc ggccgggaag tgcaccgcca ccgggtgcgg cacccggtcc
     4441 agggagaggc gctggatgat gccgggtagc ccgagactgt ggtcgccgtg gaagtgggtg
     4501 acgcagatcc gggtcaggtc ggtggcggtg accgtggtgt gcaggagctg ccgctggctc
     4561 ccctcgcccg ggtcgaagag gatcacctcg tcgtcccagc gcagcacgta cccgttgtgg
     4621 ttgcgctgcc gcgtcggcgt ctggctggcg gtgccgagca ccgtcagctc gcgcatcgac
     4681 accgtgggcc tccctgcgga catgaagcga cccccggggc ttccgcgctg gcgcgcggac
     4741 cggctggact gggccggcac cccgggggtc gggtggctgt tgtggtttcg ccgcaccgct
     4801 gccacacggt ctcgacgatc ttgcacgccc gccccggagg gcggacagtc gactgtcaag
     4861 gacagcggct agcggacagc cacctcacga gtccgataac tacacaaagc ttcggatcac
     4921 ctcccttccc gtgtaccgcc acgctatcca cttcccgacg ccggcggcaa cggttttacg
     4981 tcacctgctg ccgacgcagg tcaggacggg tgcggggcag ggtcggcgat gccgatcggc
     5041 ccctcgcgcg gcccctcctc ggacgccggc tgcaccgcca gcgacgggaa gtcggccagg
     5101 tcgccctcgg gctcggcgtc ccactccggg aagaccaggt cgctctggag gtagaggcag
     5161 agcagctggg tcaggtggcg caggccgtcc aggtgggtgc gctcgtggcc gtgggtggcg
     5221 tccaccccga agccgagcag cgccacccgg gcgtgcgcgc ccgcctcgac cgccgccgcc
     5281 acgtccgagc ggtagtagtc gaagacgtcc cggaccaggt cgacgccgtg ctcggcggcg
     5341 atcgaggcga ggttgcgggt caggtggtag tcgaacgggc cgaccccgtc gcccatcgcc
     5401 agggtcgccg cggtctcccg ggactgctgc cccggggcga ccaccgccgc gtccaccgag
     5461 acgatctccg cgacatccgg gtcgaggccg tggctcgccc cgtgcccgat ctcctcggtg
     5521 acggtgacca gcaggtgcgc ggagacccgg ggctggacgc ccgcgtcgac catcgccttg
     5581 agcgcggtca gcaccgccgc cacgccggcc ttgtcgtcca ggtggcggga cttgacgaag
     5641 ccactcgggg tgatcgtcgg gttgggcagg aacgccacga agtcgcccgc gtcgatgccg
     5701 agcgcccgca gcccggcgat gtcctcgacc ggctcgtcga cccggacctc cacctgctcc
     5761 cagccgacgc cctggaggtc gaccgcctcg ttgtagcggt ggccgctcgc cttcagcggc
     5821 agcacctggc cggtgatcac ccggtccagg tcgtcggtga agatccgtac gtgggcgccc
     5881 tcggcgaagc gggcgctgtg cgtaccgatc gacttcagct ccagccggcc gttctccttc
     5941 agccgcttca ccatcccgcc gatcgtgtcg gtgtgcacca cgatcgcccg gtccgccccg
     6001 gtggagcggg ggccgggcag gcaggcgctc agtgcgcccc gccgggtcag cgtggacgtg
     6061 atgccgagcg cggagagccg ctcgccgacg tactgctgca cgtggtcggt acgccccgac
     6121 gggctgggga tctccaggag ttccagcagc acctgccgca ggtagtccag gtcgagcgga
     6181 agcggggcgg gacgacgttc ggtcatgccg tcgaggatgc cgtacccgcc ggggaccaga
     6241 gccggtgcgg tgcccgggtg cccgggaaca gcaggtcgac gaagcgctcg gcggtcggtt
     6301 ggggctcgtg gttggccagt cccggccgct cgttcgcctc gatgaacacg tgttccggct
     6361 ggtccggggc ggtgaccagc aggtccagcc cggtgaccgg gatgtccagc gcccgggtgg
     6421 cggccacgca cgcctcggcg atctccgggt gcaggtcgcc ggtcacgtcg tggatggtgc
     6481 cgccggtgtg caggttggcg gtccgccgga cggcgatcat cctgccctcg gggagcacgt
     6541 cgtgcatgcc gtacccggcc tcggcgacca cctcgcgggt catgtcgtcg atcgggatgc
     6601 gggactcgcc gccggtggcg gcggcccggc ggcggctctg ccgctcgatc agctcggtga
     6661 cgtcgtgcac cccgtcgccg gtgatcgagg cgggctggcg gacggccgcc gcgaccacct
     6721 cgtggtcgat caccaccacc cgcaggtccg caccggcgcg cagttcctcg atcagcacgt
     6781 ccgggcagta ccgccgggcc agttcgacgg ccgtgcgcag cgcctccggg gcccgcaccc
     6841 cgacggtgat gccgttgccc tgctcacccc gggccggttt gaccaccacc gcgccgatct
     6901 cggccaggaa cgcctcgtcc gcgtcgtccc cggtggccgt ccggccacgc ggcacgctca
     6961 gtccggcctc ggtgaggatc cgccgggtca cccgcttgtc gtcgcagcgg ctcatcgcca
     7021 ccgccgaggt cagctcggag agcgactcgc gggtcaggat ggtccgcccg ccgctggtca
     7081 gccgcagctc gccccaggcc gggtcggtca cctccacccg gattccccgg cgcatcgcct
     7141 cgtcggcgac gatccgggcg tacgggttca gctcgtcgta gccggcgggc agggacggca
     7201 ggaaaagtcg ctcgttgatc gggttcttcc gcttcacgca gagcgtggcg gtgcggtaga
     7261 aacccagccg ctcgtagagg cggatcgccc cgtcgttctc cgccagcacc gacaggtcga
     7321 cgaaggcccg accccggtcg gtgagccggg cggccagctc ggtcaggagg gcctggccgg
     7381 taccgggcgg ggcgtggttg aagtccacgg tcaggcacca caggctcgcg ccgttgtccg
     7441 ggtcgtcgaa gacctcgacg tggtcgacgc cggtgatcgt gccgacgatc tccccggtgg
     7501 ccgactcggc gaccaggtgc aggaaggacg gggtggcggc gttggcgacc agcacgtcca
     7561 ccggggcggt gaccatcccg ttgctggcgt agatccggtt caccgcgtcg gcgtccgccg
     7621 cgtcgcggag ctggcggaca cgcagccccg gcaggtccgc gccaccgtcg gcggccggct
     7681 cgccaccgat cgggccggga ccgggggccg cgtccgggct cagcggcagc cggtaggtca
     7741 gcgacgggtc gatgaacagc tcgtcgtgca gccgggagac cagcacgtgc gggtcgcgca
     7801 ggtagatgca gatgtcccgg gaaccggcgg cctccgagcg gagcacgtcg gcgacgtcgg
     7861 ccgggtcgcg gaacgtctgg ccgaagacca gccgccccca gccgcagtcg agcaccacgt
     7921 cggtacgacc ggcgggcggg acagcatccg ggtcggtcga ccggccggcg ggctcgccgg
     7981 gggcgaccgg gtcaccgccc ggcccgaccc gctcccgtcg ccgggccatc cgggcccgct
     8041 gcccctcggt cagggccgcc cccgtcgcca gggtctcggt cacggtcagg cgattccgtg
     8101 gctctggagc cacatttcca ggagtccgag ctgccacagc ttgtttccgt tcaacggggt
     8161 gagttcggcg ttcgggtcgg tgaggagggc gtcgacgtag tcggcgcgga acaggtcgcg
     8221 gcggcgagcc tccggggcgt gcagcgcgtc gcggacccgg tcgaggacct tgccctcgag
     8281 gtgggtgagg ccgggcaccg ggaagtagcc cttcgggcgg tcgatgacct cccagggcag
     8341 cacccgacgg ccgatctcct tgagcacgcc cttgccgccc tgggcgagct tcagctcggg
     8401 tgggcagctc gcggcgagct cgacgaactc gtggtccagg aaggggaccc gggcctccag
     8461 gccgtgcgcc atcgtcatgt tgtcgacccg cttcaccgga tcgtcggtga gcatgatctg
     8521 ggtgtcgatc cgcaggcccg cgtcgaccgc ggtgtccgcg cccacccggc ccaggtgcgc
     8581 ggccacgaac tcgcgcgccg ggtcaccgtc ggccaaccgg tccgggttga ccacccgggc
     8641 cagtccggcc gcgtcccggt cgaagaacgc cttcgcgtac gtgtcgagag cggccgcccg
     8701 gtcgacgccg gccagcggcg ggtaccagtg gtagccgccg aggatctcgt ccgcgccctg
     8761 cccggactgg accaccttga cgtgccgggc gacggtctcg ctgagcaggt agaacgccac
     8821 gcagtcgtgg ctgaccatcg gctcgctcat cgccgccacg gccgcctcca gcggcgggac
     8881 gaggtcgttc gtcgcgaccc ggatctggtg gtggtcggtg tcgaaggtct tcgcgatcag
     8941 gtcggagtag cggaactcgt cgccctcccg gccgccgacc gcctcgaagc cgatggagaa
     9001 ggtggccagg ccgcgctgcc cctgctcggc gaggagcgcc accaccaggc tggagtccag
     9061 gccgccggag aggagcaccc ccaccggcac gtcggcgacc atgcgtcgcc ggacggcggt
     9121 ggtgagcgac tcgtggaggg cgtcctgcca gtcccgctcg gaccagccgg cccggtccgg
     9181 gtggcgggtg aacgacggat cccagtagac ccgctcggtg acccggccgt ccggctcgta
     9241 cacccggacg gtggccgggg gcagcttggc cacgccgcgc aggatggtcc ggggcggtgg
     9301 cacgatgctg tggaagctca ggtagtgcgc gagcgcgacc gggtcgaccg tggtgtcgat
     9361 cccgccaccg gccagcaggg cgggcagggt gctggcgaac cggacccggt cgggggtctc
     9421 ggccaggtag agcggcttga tgccgagccg gtcccgggcc agcaccaggc gaccggtggc
     9481 ccgttcggtg atcgccacgg cgaacatgcc gaccaggtgg tcgacgaagt cgtcgcccca
     9541 ctcggcgtac gccttgagca ccacctcggt gtcgcccgag gagaagaaga cgtgtccctt
     9601 ggcctggagc tcggcgcgca gctcccggta gttgtagacg cagccgttga agacgccggt
     9661 cagcccggcg gccgagtcga ccagcggctg cccgctcgcc gccgacaggt cgatgatctt
     9721 gaggcgacgg tggcccaggg cgaccggccc ctgcgaccag acgccgctgt cgtcggggcc
     9781 ccggtcgtgc atggtcgccg ccatccgctc caccgccgtg acgtcggcac gtgagccgtc
     9841 ccgacgaagc tccccagcca gtccgcacat gccaaccgat ggtgccagag gtggcagcgc
     9901 atcgcctgtt gagctgatag cccttcgggt ggccagttgc tagaatgccg cgccccgtgc
     9961 gggctggagt ggatctacgg agtttccgtg tcccgcaggg gaggagtgac ccttttgtgc
    10021 ccgcgcactg ttgtgaacaa gacctcagcc tgcgtccggc ggtcgacagg tgcccgctga
    10081 acgtcgagga gcggtcgccg ggacgggcac gacgaccagc tgggcgtgaa ccacgccggt
    10141 cgccgtaccc ctcccgtacg tcgtgggcag ggctgcggtc agccgctggt gcgggccacg
    10201 ccgaccgggc agctcatccc gttggggccg tggttgcagt agccgttcgg gttcttcgtc
    10261 ggggcgaggt actgctggtg gtagtcctca gcaaagaaat agtcgccgag ctggtcgatc
    10321 tccgtggtga tctcaccccg gccggcacgg gccacgatcg gggcgaacgc gtcccgggag
    10381 gccttcgcgg tggcggcctg ctcgtcgctg gtggtgtaga tggtcgaccg gtactgggtg
    10441 ccgacgtcgt tgccctggcg catgccctgg gtcgggtcgt ggttctccca gaagaccttg
    10501 agcaggtcct cgtacgcgat cgtcgtgggg tcgtagacca cctggaccac ctcggcgtgc
    10561 ccggtacgac cggagcagac ctcctcgtac gtcgggttcg gggtgtagcc gcccgcgtac
    10621 ccgacggagg tggtgatcac gccggggagg ccccagaaca ggcgctccgc gccccagaag
    10681 cagcccatcc cgaagaccgc cacctgcgcg ccctcgggga acgggccctt cagcggggtg
    10741 ccgagcacct cgtgccggtc gctgaccggc atcgccaggg ggcggccggg cagggcctgg
    10801 tcgggcgtga tcatttcggc cttcatgcgg cgcaggaaca cgatgactcc tctcgtatct
    10861 gtctatcctg caacgccggc gggtcccccg tccttacccg ttcagccgcc ggtcaggcga
    10921 tcacgggcgg tccaggcgac gaaccgctca ccgcgtcggc gtcgggcggg gggaacattc
    10981 gggtccgacc tcctgtcgag ctgccccgtc cgtgcgcccg cccggccgga gggcgggaca
    11041 cctgtgcgcc cgccccggcc agggctgccc ccgctgaccc cggcgggaaa aaccgctgcc
    11101 ccgaccagcc gatcgctgcc accatctccg ctgatggaga tcagggagat ggagccgggc
    11161 acggacgagg cggtggcgcg gtcgctgctg cgggtgcagc gggccgcgta cgcggtggag
    11221 gcggtgctga tcggcgacga ccgcatcccc acgctgaggg agaccctcga cgagcttcgc
    11281 gccgcgcccc tgcgctggct cgtcgccttc gccgatgacg cggcgaccga agacgaggtg
    11341 gccgaccgcg tggcaaccga tcacacggcg gccgacgacg cggcggccgg cgggtccggt
    11401 ggaggtcgcc tggtcggcgc ggtggcctgg acggaggacg cggcgacggt cgacatcgac
    11461 cggctcgtgg tcgacccggc cgtccaccgg cgcggcgtcg gccggatgct ggtcggtgag
    11521 gtgctgaccc gggccggcgg ccgtaccgtg gtcgtcgcca ccggccgggc gaaccggccg
    11581 gcgcgggcgc tctacgagtc gttcggcttc gtggtgctcg gtgacgccga acccgtcccc
    11641 ggcctctgga tcacccgcta cacccgacca gggccggagg agagagccgc cgcgacgcgg
    11701 tcggcgtagg actgcgcctc ggcgtcgtcg gtgtaacgcg tccggggcca gaagaagccg
    11761 cgcaacccgt cccccttcgt ccggggcacc acgtgggtgt gcaggtgcgg caccgactgg
    11821 gagacccggt tgttcatcgc cacgaaggtg ccgtccgcgc ccagcccggc ctcgaccgcc
    11881 acggcgagcc ggcggaccag cccgaagtac ccggcgatgg actccgccgg caggtcggcc
    11941 agggtcacca ggtgggcccg gggcgccacc agcacgtgcc cccggaacac cggccgggtg
    12001 tccaggaacg ccaccccgtc cgcctcgtcg gtgacccgga acgccggtac cgtaccggcc
    12061 acgatgccgc agaacacgca cccgctcatc cgggcaggct aatgcctcac gtggccgccg
    12121 ggccgggccg cgcggcgcac ttcacggatc gcgtatcggc cggggcacta gcctgtcggg
    12181 aatgaaccac ggtttcgaga cgctcgcgat ccacgccggc caggacccgg aggcccgcac
    12241 cggcgcggtg atcccaccga tctatcagac cagcacctac gcccaggacg ccgtcggtgc
    12301 gccccggctg ggttacgagt acagccggtc cggcaacccg acccgcgacg ccctccagga
    12361 gtgcctcgcc gcgctcgagg gcggcccggt cggcctcgcc ttcgccagcg gcctggctgc
    12421 cgaggacacc gtgctgcgga ccgtctgcaa gccgggcgac cacgtggtca tcccggacga
    12481 cgcgtacggc ggcacgtacc ggctcttcgc gaaggtcgcc gagcgctggg ggctggcgta
    12541 caccccggcg aaggtctccg acccggacgc cgtacgggcg gcgatccgcc ccggcagcac
    12601 gaagatcgtc tgggtggaga cgccgaccaa cccgctgctc ggcatcgccg acatcgccgc
    12661 gctggccggg gtcgcgcacg acgccggggc gctgctggtg gtcgacaaca ccttcgcctc
    12721 gccgtacctc cagcagccca tcgcgcacgg cgcggacgtg gtggtgcact ccaccaccaa
    12781 gtacgtcggc ggtcactccg acgtggtcgg tggcgccctc gtcgcggccg accgggagct
    12841 gggcgagcag ctgcgctacc accagaacgc gatgggcgcg gtcaacggcc cgttcgacgc
    12901 ctggctcacc ctgcgcggcg tcaagaccct cggcgtccgg atggaccggc actgcgacaa
    12961 cgccgagcgg atcgccgcct atctggacgg gcacgcgaag gtggccgagg tgatctaccc
    13021 cggcctgccg tcgcaccccg gccacgaggt ggccgccaag cagatgcgcc ggttcggcgg
    13081 catgatctcc ttccgggcca ccggtggcga ggagcacgcg gtcgagatct gcaaccgcgc
    13141 ccggctcttc gtcctcgccg agtccctcgg cggggtggaa tccctgatcg agcacccggg
    13201 ccggatgaca cacgcaagtg ccgccggctc gccgcttgaa gttcccggcg atctcgtgcg
    13261 actgtctgtc ggcatcgaga cggtcgacga cctgctcgcc gatctggagc aggcgctggg
    13321 ctgaccgctg gctggggagg gtggccgtgc aggacatcat gccgacctgg gtcggggcga
    13381 ccgcgaagca gatcgcccgg ggcgtacgcc ggggggacgt ctcggcgacc caggtggtcg
    13441 ccgaccacct cgaccacatc gcccgggccg acgccgacct cgccgcgttc cgcacggtac
    13501 gcggcgggga ggcgatcacc gaggcggaga aggtcgacga gcaggaggac ctggcgaacc
    13561 tgccgctggc cggggtgccg gtcgcggtca aggagaacac gccggtcgcc ggcctgccca
    13621 cctggcgggg ctcggccgcc gtgcgtaccc cggtcgcgga gagcgaccac gaggtggtcc
    13681 gccggctgcg cggcgcgggc gcggtcatcc tcggcgtcac ccggatgccg gaactcgggt
    13741 tgtgggcggt gaccgacgac gagacggccg tcacccgcaa cccgtgggac ccgggccgga
    13801 cgccgggtgg ctcgtccggc ggtgccgccg cggcggtggc cgccggactg gtgccgatcg
    13861 cgcacgccaa cgacggtctc ggctcgatcc gcatcccggc ggcctgctgc ggcctggtcg
    13921 ggctcaagcc cggtcggggt gtggtgccct ggcagctcgg cgcggaggac tggttcgggc
    13981 tggccgagca cggcatgctg accaccacgg tcgccgacgc ggcggtgggc ttccaggtgc
    14041 tcgccggccg tccccaggag aagctggtgc ccccgcagcg gctgcgggtg ggtgtctcgc
    14101 tgcgctcgcc ggtgcgcggt atcgccgccg accaccccaa ccgggacgcg gtggccgccg
    14161 ccggccggct gctcgccgcc gccggccacg acaccgtgcc ggccgacccg gtgtacccga
    14221 ccgcgctcgg gctcaagggc gtcgcgacct ggctcgccgc cgccgccgtc gacgtccggg
    14281 ccggtggcct ggaacgccgg cacctccagc ggcgcagccg gcggcacgtc gccctggggg
    14341 agtgggcgca gcgccgggga tacgtccgcc aggccgaccg ggacgcctgg cgcgaacggt
    14401 cgatcggctt cttcaccgac cactcggtcg acctgctgct cacccccgcg ctggccagcg
    14461 cgccgccggc agcgcagacc tggtcgggcc ggtcctggcg gtcgaacatg agcaccagca
    14521 tccggtacgc cccgtacgcc gcgccgtgga acatcgccgg cctgccggcg ctggtggtgc
    14581 cggtcgggcg gcgtcccgac ggcctgccgg tggcggtgca gctggtcggc ccgcccggct
    14641 ccgagctgct gctgctcggt gtggccgggc agttcgagat gcaggcgccc tgggcgcggc
    14701 atgcccccgg gtacccgcgc gtcgggacgg gatcgccggc cgccgcgtag tcgtccagtg
    14761 tgggccggcc ccgacaacgg gccggggcca ccgtccgcga tgccgtcccc aggagcaggt
    14821 ggcaccatca gggcatgacg gagctggtcg gcctcgccga cgtccaggca
//
