LOCUS       NZ_BNBR01000010        12937 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces griseosporeus strain JCM 4766 sequence10, whole genome
            shotgun sequence.
ACCESSION   NZ_BNBR01000010
VERSION     NZ_BNBR01000010.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces griseosporeus
  ORGANISM  Streptomyces griseosporeus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 255716)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 112X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014656055.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 05:04:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,365
            CDSs (total)                      :: 7,282
            Genes (coding)                    :: 7,186
            CDSs (with protein)               :: 7,186
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 61 of 96
            Pseudo Genes (internal stop)      :: 11 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:46:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 57457
            Orig. end                         :: 70394
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNBR01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12937
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="2"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12937
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12937
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7937](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7937
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(494..970)
                     /locus_tag="IE308_RS35450"
                     /old_locus_tag="GCM10018783_71710"
     CDS             complement(494..970)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1298: putative carboxymuconolactone decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139751.1"
                     /locus_tag="IE308_RS35450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_71710"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_110627792.1"
                     /transl_table=11
                     /translation="MDARLNLHENAIAAKFFKRLNAAAAVVAEAGLPERTGNLVVLRAS
                     QINGCGFCTDMHTKDALAAGEDSTRLALVAAWREATLFTDAERAALELTEEATRIADAA
                     GGVSDEVWERAARHYDEDQLVALICLIGVINAYNRANVVVRTPAGWYEPGMFAA"
     misc_feature    1049..1064
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     gene            complement(1060..2460)
                     /locus_tag="IE308_RS35455"
                     /old_locus_tag="GCM10018783_71720"
     CDS             complement(1060..2460)
                     /EC_number="3.4.11.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1172: methionine aminopeptidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743074.1"
                     /locus_tag="IE308_RS35455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_71720"
                     /product="aminopeptidase P family protein"
                     /protein_id="WP_190221028.1"
                     /transl_table=11
                     /translation="MSDTAPPLRTGSHDLSVSTELARFMGRDWAPTPLPQDARVAARAV
                     TPARRARLSARFPGERLVIPAGELKVRSNDCDHRFRPYSAYAWLTGLTGEDQPGHVLVL
                     EPSGPHDHEAVLYIRPRSPRTGRAERPADAFYRDRRHGEFWVGRRPDLAETERLTGIRC
                     ADLDEPGGTLPPGREPSTDPELAAVLSELRLIKDDWEIDQLQQAVDHTTAGFEDVVRSL
                     PRALAHPRGERWIEGVFGLRARAEGNGLGYETIAASGAHACVLHWTRNDGALNPADLLL
                     LDAGVETDTLYTADITRTLPLSGRFSPVQRQVYELVLAAQEAGIAALRPGALFRDFHRA
                     AMRVIAEGLAEWGVLKDAEGDLHRRYTLCSSGHMLGLDVHDCAQARAGAYLDGVLEEGQ
                     VLTVEPGLYLQPDDLTLPPELRGIGVRIEDDLVVTSDGARLLSSALPRTPDAVEEWMGT
                     LLDSTTTQ"
     misc_feature    2495..2511
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.29"
                     /tool="antismash"
     misc_feature    2499..2509
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    2503..2516
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.09"
                     /tool="antismash"
     gene            2611..4932
                     /locus_tag="IE308_RS35460"
                     /old_locus_tag="GCM10018783_71730"
     CDS             2611..4932
                     /EC_number="3.4.24.3"
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890144.1"
                     /locus_tag="IE308_RS35460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_71730"
                     /product="collagenase"
                     /protein_id="WP_373314676.1"
                     /transl_table=11
                     /translation="MFHQRTALLAAAVITATATLLGASGPSAAHAVGNGLGPALSVPAG
                     RQPGDDVDHLGKAPARVAPTAQAPGAHRADGRVPGAAAPRTAGARTAAAPRTAVAPCTL
                     DGVTGLAPEPFADFLAAPDVTADGCLRDILWTWDARLAPVMDDAHVQAVARRATALAAR
                     HDGRDSSHLLEMFTYLHAVAYHDFARDEVDISDAPTLEAVRRAVYYFGTAPRTFDATRA
                     NAETLREALYTGSAPGLRQSQLDLIRKVLATLDRHHTSTYADPSWGGAALAALSVNYLG
                     LYEGNHDTAFRTLAARNDAYRDAFRRFAEYTHLKGTPNEWAVRDALSEYARFGTVASLR
                     GRIVADLGALLAPVTDDWGRGSAPWAKVVSWLIHYEACAPYAVCADDIERQLFPQTYRY
                     DNGALTVRTALDRATVDQLYYASKQVKAQFHRVLGARAPLAGDTNTVLTVVLYASRADY
                     ENYHPLLTGMGTNNGGIYIERGATFYTYQRRVPQDSRLTLEELFRHEYVHYLNGRWAVP
                     GYFGEGPWYQGDRTTAMDEGTAEFFAGSTRADGVAVRRSLVEGIIRDTANGGPRMTVDR
                     LLHATYDGDGFRFYDYAGTFFDYLWTEHPSLLREMYRYLRADDPAAFDAWRDRAGRDAA
                     LQQGYDAFLDRQIARVTDLYVPRTSFTPNDGLREATADAVRSAFAAATSADPACAADGD
                     PGMPRFACTGRITARLTDAGSADLVFRDMTETVDAYLLDRAAPASPNLADMTCSFGEVT
                     IWSDRTGGTADYTCEGPLRR"
     gene            5001..6233
                     /locus_tag="IE308_RS35465"
                     /old_locus_tag="GCM10018783_71740"
     CDS             5001..6233
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850110.1"
                     /locus_tag="IE308_RS35465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_71740"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_190221145.1"
                     /sec_met_domain="DAO (E-value: 2.7e-72, bitscore: 238.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPSLDAVIVGAGVVGAACAYYATRAGLSVAVVDRGPVAGGTTG
                     AGEGNLLVSDKEPGPELELALLSTRLWRELAAALPPEDAVEYEPKGGLVLAPDETALTA
                     LRAFAAGQCAAGVEAREVDEHELAHLEPELTKGLAGGFHYPQDAQVQPARAAAALLRAA
                     RSRGALVRLGEEVTAVLTGPHGAVRGVRTPRGELHAPAVVNATGTWGGRLAALAGTRLP
                     VLPRRGFVLVTEPLPPGLIRHKVYAADYIADVASDSAALQSSAVVEGTPAGPVLIGATR
                     ERVGFDRTLSTEALRRLAGQATALFPRLADVHAIRTYCGFRPYLPDHLPAIGPDPRVPG
                     LHHACGHEGAGIGLAPATGLLVTAALTGDEPPVGAWPFRPERFDSRGPADDFSDPFDRP
                     DGPPPSPGART"
     gene            6230..6541
                     /locus_tag="IE308_RS35470"
                     /old_locus_tag="GCM10018783_71750"
     CDS             6230..6541
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038322.1"
                     /locus_tag="IE308_RS35470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_71750"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_190221030.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.1e-26, bitscore: 86.6,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQTPLGLVRAAPAAPYTFTFDGRILTALPGQTLAAALWAAGIMS
                     WRTTRGGGAPRGVFCGIGVCHDCLATVNGRPNQRACLVAVQPGDVVTVQEGTGHDATR"
     gene            6525..7937
                     /locus_tag="IE308_RS35475"
                     /old_locus_tag="GCM10018783_71760"
     CDS             6525..7937
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237884.1"
                     /locus_tag="IE308_RS35475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_71760"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_190221031.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.8e-27, bitscore:
                     89.1, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 1.3e-12, bitscore:
                     41.8, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPPVDLAVVGAGPAGLAAAVRAAGLGLSVALLDAGERPGGQFHR
                     YPAPGLRAARPEALHHGWRHFARRLRQLETGGVTYLPRHHVWTVARGDGCWLLHAVVGP
                     DETPATVRARAVLLATGAYERQLPFPGWTLPGVVGAGGAQAMLKSGLVLPGRRVVVAGS
                     GPLLLAVAASLAAAGARVPAVVEAADYRAYGRSLPAVLRTPAKLAEGARYGGGLLRRST
                     RLLTRHAVTAAHGTARVEAVTVTRLDDHWRPVPGTGRRVPCDALAVGHGLVPQLDLAVG
                     LGCATRRTADDTHALVVDADQATTVPGIWAAGESCGIGGADLALTEGEIAADAVARTLH
                     DVPADPRRHAALTRRRTRLRAFADAMAAAHRPGPGWADWLDDATEVCRCEEVTAGRVRE
                     AVAGYGAHDTRTVKLLTRAGMGWCQGRMCGQAVARLAGGEGAAAVESRPLSCPVPLRPL
                     AQLPPEAEEFRA"
     gene            7934..8878
                     /locus_tag="IE308_RS35480"
                     /old_locus_tag="GCM10018783_71770"
     CDS             7934..8878
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543026.1"
                     /locus_tag="IE308_RS35480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_71770"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_190221032.1"
                     /transl_table=11
                     /translation="MTVPDTRPRPWHGVMVATALPLRPDLGVDYDAYAAHVARLVDAGC
                     DGVVPNGSLGEYQTLTDDERARVVRVAVEAAGDGARVMPGVSAYGGAEARRWAEQAAEA
                     GCGSVLLLPPNAYRADERAVRAHYAEVARAGLPVVAYNNPYDTKVDLTPELLARLHGDG
                     HIVAVKEFSGDVRRAYEIAELAPELDLLIGADDVLLELALAGAVGWIAGYPNALPHACV
                     DLYRAAVARDLERALPLYRALHPLLRWDSKTEFVQAIKLSMDLAGLPGGPTRPPRGPLG
                     EAEAAAVRAATEKVLAEGFGEASGLSGLSGEVR"
     gene            8878..9882
                     /locus_tag="IE308_RS35485"
                     /old_locus_tag="GCM10018783_71780"
     CDS             8878..9882
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="IE308_RS35485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_71780"
                     /product="proline racemase family protein"
                     /protein_id="WP_190221033.1"
                     /transl_table=11
                     /translation="MRTRHVYHAVDSHTEGMPTRVVTGGVGVLPGATMAERRRYLVEHL
                     DHVRTLLMYEPRGHAAMSGAILQPPTRPDADWGVLFIEVSGALPMCGHGTIGVATVLVE
                     TGMVPVVEPVTTVRLDTPAGLVSVDVRVADGVAESVTLTNVPAYCAGLGRTVEVPGYGT
                     VAYDLAYGGNFYAFVELDALGLPYERERKGELLAAGLAIMDAVNGGADRPVHPENPDIA
                     GLKHVYLIAPGSDAKHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLGADFVNE
                     SFIGTRFVGRLIGDAAVGGVPAVVPAVTGRAWITGTAQYFLDPEDPFPAGFLL"
     gene            9964..10632
                     /locus_tag="IE308_RS35490"
                     /old_locus_tag="GCM10018783_71790"
     CDS             9964..10632
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938955.1"
                     /locus_tag="IE308_RS35490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_71790"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_190221034.1"
                     /transl_table=11
                     /translation="MGGHLKQANLITAQERLRDQVAHALRAALISGELQPGRVYSAPAL
                     ASDFGISATPVREAMLDLVREGLVEPVRNKGFRVTEVSEKDLDQYSELRALIEVPTVGA
                     VTRSASREQLEALRPVAEEIVATAREHDLIGYLDADRRFHLSLLALHGNDRLVETVGDL
                     RKRSRLYGLTALDERGLLVASAEEHLELLERMLAGDGEGAEAVMRRHLGHVRALWAAES
                     "
     misc_feature    10789..10805
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.88"
                     /tool="antismash"
     misc_feature    complement(11221..11234)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.39"
                     /tool="antismash"
ORIGIN
        1 tccgtggcgc gcctgcggta ctcctggacc gcccagccgt tgccgtcggg gtccttgaag
       61 tacaggaagg tggcgccgtc gtccggggcg taactcaccg gctcggagac gtccaggccc
      121 cgctcggtca gctccgcgtg ggcggccttg atgtcggcca cgcacagctg gaggccctgg
      181 taggagccgg gggccgggcg cgggccgccg gccatctccc agatgctgtc gccgagcgcg
      241 atcgaacagc cggagcccgg gggcgtcagc tggacgatcc gcatccccgg catgacctcc
      301 gtgtcgatgt cgacgtggaa gccgaccttg tcgcggtaga agtcccgggc ccggtcgatg
      361 tcgctcaccg gcagggggat cacttcgagg gtgaagtcca tcgttccgcc tcctggaggc
      421 tcgtggtggt ccccttcacc gaagcggaaa accgggcccg gcgccaccgc atcggcctgc
      481 cggccgcgtg gatctacgcc gcgaacatgc ccggctcgta ccagccggcc ggtgtgcgca
      541 cgacgacgtt cgcccggttg taggcgttga tcacgccgat caggcagatc agcgccacca
      601 gctggtcctc gtcgtagtgc cgggcggccc gctcccagac ctcgtcggac accccgcccg
      661 ccgcgtcggc gatccgggtg gcctcctcgg tcagctccag ggccgcgcgc tcggcgtcgg
      721 tgaacagcgt cgcctcccgc caggccgcga ccagggcgag gcgggtggag tcctcgccgg
      781 ccgcgagcgc gtccttggtg tgcatgtcgg tgcagaaacc gcagccgttg atctggctgg
      841 cgcggagcac caccaggttc ccggtgcgct ccggcaggcc ggcctcggcg accaccgccg
      901 cggcggcgtt gaggcgcttg aagaacttgg cggcgatcgc gttctcgtgc aggttcagtc
      961 gggcgtccat cgcggtgttc ctccgtcgtc gtgcgagtgg gtacgccggt gtgacggagg
     1021 aacccgacgc gatgtgacgg ccgagcgatg tgacctgggt cactgtgtgg tggtgctgtc
     1081 gaggagcgtc cccatccact cctccaccgc gtccggtgtg cgcggcagcg ccgaggacag
     1141 cagccgtgcg ccgtcggagg tgacgaccag gtcgtcctcg atccgcacgc cgatgccgcg
     1201 cagctcgggc ggcagggtga ggtcgtcggg ctggaggtag aggccgggtt cgacggtgag
     1261 cacctggccc tcctccagga cgccgtccag ataggcgccg gcccgcgcct gggcgcagtc
     1321 gtgcacgtcc aggccgagca tgtgcccgct gctgcacagg gtgtagcgcc ggtgcagatc
     1381 gccctcggcg tccttcagga cgccccactc ggccagcccc tccgcgatga cccgcatcgc
     1441 ggcccggtgg aagtcgcgga agagcgcgcc gggccgcagc gccgcgatac cggcctcctg
     1501 cgcggcgagg accagctcgt acacctggcg ctggacgggg gagaagcggc cggagagggg
     1561 gagcgtgcgc gtgatgtcgg cggtgtagag ggtgtcggtc tccacgcccg cgtcgagcag
     1621 cagcaggtcg gccgggttca gcgcgccgtc gttgcgggtc cagtgcagca cgcacgcgtg
     1681 ggcgccggac gccgcgatcg tctcgtaccc gaggccgttg ccctcggcgc gggcgcgcag
     1741 cccgaagacg ccctcgatcc agcgttcgcc gcgcgggtgg gcgagggcgc gcggcaggga
     1801 ccgtacgacg tcctcgaagc cggccgtcgt gtggtcgacg gcctgctgca gctggtcgat
     1861 ctcccagtcg tccttgatga ggcgcaactc cgacagtacg gcggccagtt cggggtccgt
     1921 ggacggttcg cggcccggcg gcagggtgcc gcccggctcg tcgaggtcgg cgcagcggat
     1981 accggtcagg cgctcggtct cggccaggtc ggggcggcgg ccgacccaga actcgccgtg
     2041 gcggcggtcc cggtagaagg cgtcggcggg ccgctcggcc cggccggtgc gcggcgagcg
     2101 ggggcgtatg tacagcaccg cctcgtggtc gtgcgggccc gacggctcca ggaccaggac
     2161 gtggccgggc tggtcctcgc cggtgaggcc ggtcagccag gcgtaggcgc tgtacggccg
     2221 gaagcggtgg tcgcagtcgt tggagcggac cttcaactcg ccggcgggga tcaccagtcg
     2281 ctcgccgggg aaccgcgccg acagccgggc ccggcgggcc ggggtgaccg cgcgggcggc
     2341 gacgcgggcg tcctgcggca gcggtgtcgg cgcccagtcg cggcccatga aacgggccaa
     2401 ctccgtggac acggacaggt cgtggctgcc ggtgcgcagg ggcggggcgg tgtcggacat
     2461 gacggctcct gaagcgagga ctcctgaggg gttgtcagtg caatttcaca ttactatgtt
     2521 acagctcaca tgacagtggc atggacgcat caagctgatc gctgtcggtc cgtgccctgg
     2581 tcgagccact ccccacacga ggagccgctg gtgtttcatc aacgcaccgc cctgctcgcc
     2641 gcggcggtca tcaccgcgac cgcgaccctg ctcggcgcgt cgggcccgtc cgccgcgcac
     2701 gccgtcggca acggcctcgg tcctgccctc tccgtccccg ccggccgcca accgggcgac
     2761 gacgtcgacc acttgggcaa ggcgccggcc cgcgtcgccc ccaccgccca ggcccccggc
     2821 gcacaccgcg ccgacggccg cgtccccggc gccgccgccc cgcggaccgc cggtgcccgc
     2881 acggccgccg ccccgcggac agccgtcgcc ccctgcaccc tcgatggcgt caccggtctc
     2941 gcccccgagc cgttcgccga cttcctggcc gccccggacg tcaccgccga cggttgcctg
     3001 cgcgacatcc tgtggacctg ggacgcgcgc ctcgcgcccg tcatggacga cgcccacgtg
     3061 caggccgtcg cccgccgcgc caccgcgctc gccgcccgcc acgacggccg cgactccagc
     3121 cacctactgg agatgttcac ctacctgcac gccgtcgcct accacgactt cgcgcgcgac
     3181 gaggtcgaca tcagcgacgc gcccaccctg gaggccgtgc gccgcgccgt gtactacttc
     3241 ggcaccgcgc cccgcacctt cgacgccacc cgcgccaacg ccgagaccct ccgcgaggcc
     3301 ctgtacaccg gcagcgcgcc cggcctgcgc cagtcccagc tcgacctgat ccgcaaggtg
     3361 ctggccaccc tcgaccgcca ccacacgagc acctacgccg acccgtcctg gggcggcgcc
     3421 gcgctcgccg cgctcagcgt caactacctc ggcttgtacg agggcaacca cgacaccgcc
     3481 ttccgcaccc tcgccgcccg caacgacgcc taccgcgacg ccttccgccg cttcgccgag
     3541 tacacccacc tcaagggcac gcccaacgag tgggccgtcc gcgacgccct gtccgagtac
     3601 gcccgcttcg gcaccgtcgc ctccctgcgc ggccggatcg tcgccgacct cggcgccctc
     3661 ctcgccccgg ttacggacga ctggggccgg ggcagcgccc cctgggccaa ggtcgtctcc
     3721 tggctgatcc actacgaggc ctgcgcgccg tacgccgtct gcgcggacga catcgagcgg
     3781 cagctgttcc cgcagaccta ccgctacgac aacggcgccc tcaccgtccg caccgccctc
     3841 gaccgcgcca ccgtcgacca gctctactac gcgagcaagc aggtcaaggc gcagttccac
     3901 cgcgtcctcg gcgcccgggc acccctggcc ggcgacacca acaccgtgct caccgtcgtg
     3961 ctgtacgcct cccgcgccga ctacgagaac taccacccgc tgctcaccgg catgggcacg
     4021 aacaacggcg gcatctacat cgagcgcggc gccaccttct acacctacca gcggcgcgtc
     4081 ccgcaggact cccggctgac gctggaggag ctgttccgcc acgagtacgt gcactacctc
     4141 aacggccgct gggccgtgcc cggttacttc ggcgagggcc cctggtacca gggcgaccgc
     4201 accaccgcca tggacgaggg caccgcggag ttcttcgccg gctccacccg cgccgacggc
     4261 gtcgccgtcc gcagatccct ggtggagggg atcatccggg acaccgccaa cggcggaccg
     4321 cgcatgaccg tcgaccggct cctgcacgcc acctacgacg gcgacggctt ccgcttctac
     4381 gactacgccg gcacgttctt cgattacctc tggaccgaac acccctccct gctgcgggag
     4441 atgtaccgct acctgcgggc ggacgacccc gccgccttcg acgcctggcg ggaccgcgcg
     4501 ggccgcgacg ccgccctcca gcagggctac gacgccttcc tggaccggca gatcgcccgc
     4561 gtcaccgacc tgtacgtgcc ccgcacgtcc ttcaccccga acgacgggct gcgcgaggcc
     4621 accgccgacg ccgtgcgctc cgccttcgcc gccgccacct ccgccgaccc cgcgtgcgcg
     4681 gccgacggcg accccggcat gccccgcttc gcgtgcaccg gtcgcatcac cgcccggctc
     4741 accgacgcgg gcagcgccga cctggtgttc cgggacatga cggagaccgt cgacgcctac
     4801 ctgctcgacc gggccgcgcc cgcctcgccc aacctcgccg acatgacctg ctccttcggg
     4861 gaggtgacga tctggtccga ccgcacgggc gggacggccg actacacctg cgaggggccg
     4921 ctgcggcggt gaccgggacg cgggcacggg ctcttgtgag gcacagtgcc atgtgaaata
     4981 ttactgacgt gcccacgaag atgtccgcac cctctctcga cgccgtgatc gtcggcgccg
     5041 gagtggtcgg cgcggcgtgc gcgtactacg ccacccgcgc cggcctctcc gtcgcggtcg
     5101 tcgaccgcgg ccccgtcgcc ggcggcacca ccggagccgg cgagggaaac ctcctcgtct
     5161 ccgacaagga gccgggaccc gaactcgaac tcgccctgct gtccacgcgg ttgtggcggg
     5221 aactggccgc ggccctcccg cccgaggacg ccgtcgagta cgaacccaag ggcggcctcg
     5281 tcctcgcccc cgacgagacc gccctgaccg cgctccgcgc cttcgcggcc ggacaatgcg
     5341 ccgccggagt cgaggcgcgg gaagtcgacg agcacgaact ggcccacctc gaaccggagt
     5401 tgaccaaggg actcgccggc ggcttccact acccgcagga cgcccaggtc cagcccgccc
     5461 gggccgccgc cgccctgctg cgcgccgccc ggagccgcgg cgccctcgtc cggctcggcg
     5521 aggaggtcac cgccgtcctc accggcccgc acggcgccgt ccgcggcgtg cgcaccccgc
     5581 gcggcgagct gcacgccccc gccgtcgtca acgccaccgg cacctggggc ggccggctcg
     5641 ccgccctggc cggcacccgc ctgccggtcc tgccgcggcg cggcttcgtc ctcgtcaccg
     5701 aacccctgcc gcccggactg atccggcaca aggtgtacgc cgccgactac atcgcggacg
     5761 tcgccagcga ctccgccgct ctccagtcct ccgccgtcgt cgagggcacc cccgccggac
     5821 ccgtcctcat cggcgccacc cgcgaacgcg tcggcttcga ccgcaccctg tccaccgagg
     5881 ccctgcgccg cctcgccggc caggccaccg ccctcttccc ccgcctcgcc gacgtccacg
     5941 cgatccgcac gtactgcggc ttccgcccct atctgcccga ccacctgccc gcgatcgggc
     6001 ccgacccccg cgtccccggc ctccaccacg cctgcggcca cgaaggcgcc ggcatcggcc
     6061 tcgccccggc cacgggactg ctggtgacag cggccctgac cggggacgaa ccgccggtcg
     6121 gggcctggcc gttccggcct gagcgattcg acagccgggg ccccgccgac gacttctccg
     6181 accccttcga ccgtcccgac ggccccccgc cctccccagg agcccgcaca tgagccagac
     6241 cccgctcggc ctggtgcgcg ccgcgccggc agcgccgtac accttcacct tcgacggacg
     6301 catcctcacc gccctgcccg gccagaccct ggccgccgcg ctgtgggccg ccgggatcat
     6361 gtcctggcgc accacccgcg gcggcggcgc cccgcgcggg gtgttctgcg ggatcggcgt
     6421 ctgccacgac tgcctcgcca ccgtcaacgg ccgccccaac cagcgcgcct gcctggtcgc
     6481 cgtgcagccc ggggacgtcg tcaccgtgca ggaggggacc gggcatgacg ccacccgttg
     6541 acctcgccgt cgtcggcgcc ggaccggccg gcctcgccgc cgccgtccgc gccgccggcc
     6601 tcgggctgag cgtggcgctg ctggacgcgg gggagcggcc cggcggccag ttccaccggt
     6661 accccgcccc cggcctgcgc gccgctcggc ccgaggccct gcaccacggc tggcggcact
     6721 tcgcccggcg gctgcggcaa ctggagacgg gtggtgtgac gtacctgccc cgccaccacg
     6781 tgtggaccgt cgcccgcggg gacggctgct ggctgctgca cgccgtcgtc ggccccgacg
     6841 agacgcccgc cacggtccgc gcccgtgccg tcctcctcgc caccggcgcc tacgagcgcc
     6901 agctcccctt ccccggctgg accctgcccg gagtcgtcgg cgcgggcggc gcccaggcca
     6961 tgctcaagtc cgggctcgtg ctgcccggcc ggcgggtcgt cgtcgccggc agcggcccgc
     7021 tcctgctcgc cgtcgccgcg tcgctcgccg ccgccggggc ccgcgtcccc gccgtcgtcg
     7081 aggccgccga ctaccgcgcg tacggccgca gcctgccggc cgtgctgcgc acccccgcca
     7141 aactcgccga gggcgcccgc tacggcggcg gtctcctgcg ccgctccacc cggctgctca
     7201 cccggcacgc cgtcaccgcc gcccacggca ccgcccgcgt cgaggccgtc accgtcaccc
     7261 gcctcgacga ccactggcgg cccgtgcccg gcaccggccg ccgcgtgccc tgcgacgcgc
     7321 tcgccgtcgg ccacggcctc gtcccgcagc tcgacctcgc cgtcggcctc ggctgcgcca
     7381 cccgccgcac cgccgacgac acccacgccc tcgtcgtcga cgccgaccag gcgaccaccg
     7441 tgccgggcat ctgggccgcc ggcgagtcct gcggcatcgg cggcgccgac ctcgcgctca
     7501 ccgagggcga gatcgccgcc gacgccgtcg cccgcaccct gcacgacgtc ccggccgacc
     7561 cgcgccgcca cgccgccctc acccgccgcc ggacccggct gcgcgccttc gccgacgcca
     7621 tggccgccgc ccaccggccc ggccccggct gggccgactg gctcgacgac gccaccgagg
     7681 tgtgccgctg cgaggaggtc accgccggac gcgtccggga ggcggtcgcc gggtacggcg
     7741 cccacgacac ccgcaccgtc aagctgctca cccgcgccgg catgggctgg tgccagggcc
     7801 gcatgtgcgg ccaggccgtc gcccggctcg ccgggggaga gggggccgcc gccgtggaga
     7861 gccggccgct gtcctgtccc gtaccgctgc gccccctcgc ccaactgccg cccgaagccg
     7921 aggagttccg agcatgaccg ttcccgacac ccgcccccgc ccctggcacg gcgtcatggt
     7981 cgccaccgcc ctgccgctgc gccccgacct cggcgtcgac tacgacgcct acgccgccca
     8041 cgtcgcccgc ctcgtcgacg ccggctgcga cggcgtcgtc cccaacggct ccctcggcga
     8101 gtaccagacc ctcaccgacg acgaacgcgc ccgcgtcgtg cgcgtcgccg tcgaggccgc
     8161 aggcgacggc gcccgcgtca tgcccggcgt gtccgcgtac ggcggcgccg aggcccgccg
     8221 ctgggccgag caggccgccg aggccggctg cgggtccgtg ctgctgctgc cgcccaacgc
     8281 ctaccgggcc gacgagaggg cggtacgggc ccactacgcc gaggtggcgc gggccggcct
     8341 gccggtcgtc gcgtacaaca acccgtacga caccaaggtc gacctcacgc cggagctgct
     8401 cgcccggctg cacggcgacg ggcacatcgt cgccgtcaag gagttcagcg gcgacgtccg
     8461 ccgcgcctat gagatcgccg aactcgcgcc cgagctcgac ctgttgatcg gcgccgacga
     8521 cgtgctgctg gaactcgccc tcgccggggc cgtcggctgg atcgccggct accccaacgc
     8581 gctcccgcac gcgtgcgtgg acctctaccg ggctgcggtc gcccgcgacc tggagcgggc
     8641 gctgccgctg taccgggcgc tgcacccgct gctgcgctgg gactcgaaga ccgagttcgt
     8701 gcaggccatc aagttgtcca tggacctcgc cgggctgccc ggcggaccca cccggccgcc
     8761 gcggggcccg ctgggggagg cggaggcggc ggctgtgcgc gccgccacgg agaaggtgct
     8821 ggcggagggg ttcggtgagg cttccggcct ctcgggtctc tcgggggagg tgcggtaatg
     8881 cggacccggc acgtctacca cgctgtcgac tcgcacaccg agggcatgcc cacgcgggtc
     8941 gtcaccggag gagtcggggt gctgcccggg gcgactatgg ccgagcgccg gcggtacctg
     9001 gtcgagcatc tcgaccacgt ccgcaccctg ttgatgtacg agccgcgcgg ccacgccgcc
     9061 atgagcggcg cgatcctcca gccgccgacc cggcccgacg ccgactgggg cgtcctgttc
     9121 atcgaggtgt ccggcgcgct gccgatgtgc gggcacggca cgatcggcgt cgccaccgtg
     9181 ctcgtggaga ccgggatggt gcccgtcgtc gaaccggtca ccacggtacg tctggacacc
     9241 ccggccgggc tggtctcggt ggacgtccgg gtcgcggacg gcgtggccga gtcggtcacg
     9301 ctgacgaacg tgccggcgta ctgcgccggg ctggggcgga cggtggaggt gccggggtac
     9361 ggcaccgtcg cctacgacct cgcctacggc gggaatttct acgccttcgt ggaactggac
     9421 gcgctcgggc tgccgtacga gcgggagcgc aagggggagc tgctggcggc cgggcttgcc
     9481 atcatggacg cggtgaacgg cggggccgac cggcccgtcc acccggagaa cccggacatc
     9541 gccgggctca agcacgtgta cctgatcgcg cccggctccg acgccaagca ctcccggcac
     9601 gccatggcga tccaccccgg gtggttcgac cggtcgccgt gcggcacggg gacgtcggcg
     9661 cggatggcgc agctgcacgc gcgcggtgag ctgccgctcg gggcggactt cgtgaacgag
     9721 tcgttcatcg gtacgcggtt cgtggggcgg ttgatcggcg acgcggcggt cgggggtgtg
     9781 ccggcggtgg tgccggcggt caccgggcgg gcctggatca cggggaccgc gcagtacttc
     9841 ctggacccgg aggatccttt cccggcgggt ttcctactct gaggtgcccg agagcccgag
     9901 agcccgagag cccgcgagac ccgtaccgag ccgagacctg caaacccccc ggaggcagac
     9961 accatgggtg gccacctgaa gcaagccaac ctcatcaccg cgcaggagcg gctgcgcgac
    10021 caggtggccc atgccctgcg cgccgcgctg atctccgggg agctgcagcc ggggcgggtg
    10081 tactcggcgc cggcgctcgc ctcggacttc ggcatctcgg cgacgcccgt gcgggaggcg
    10141 atgctcgacc tggtgcggga ggggctggtc gagcccgtcc ggaacaaggg gttccgggtg
    10201 accgaggtga gcgagaagga cctcgaccag tacagcgagc tgcgggcgct gatcgaggtg
    10261 ccgacggtgg gggcggtcac gcggtcggcg agccgggagc agctggaggc gctgcggccg
    10321 gtggcggagg agatcgtggc gacggcgcgg gagcacgacc tgatcgggta cctggacgcc
    10381 gaccgccgct tccacctgtc cctgctggcg ctgcacggca acgaccggct ggtggagacg
    10441 gtgggggacc tgcgcaagcg gtcccggctg tacgggctga cggcgctgga cgagcggggg
    10501 ttgctggtcg cgtccgcgga ggagcacctg gagctgctgg agcggatgct ggcgggggac
    10561 ggggaggggg cggaggcggt catgcgacgc catctggggc atgtgcgggc gctgtgggcg
    10621 gcggagtcgt gaggcgtctg ggggtgggtg gttgctcgcg cagttccccg cgcccctgaa
    10681 tgcctgcggc ggcccgtttc tgtccgtggg tggttgctgt agcgcctgcg gctggtggtg
    10741 gggtcggggc cgcgccgggg ggtatccgtc ctcggtgcgc cgctgcgtcg gtcaaattct
    10801 taccgccact ttccgccgcc cgctgcgggc ggacaccccc cgacacgtcc cctgctcgcc
    10861 gtacgcgtct accggccgcc gtgactacgg cagcgccgcc ccgcttgggt agcgggtagc
    10921 cggcgccgcc tcgcgacttg gctgcgggca gccgccccgc tgggccggcg cccaccccgc
    10981 ggcggcgggc cgcacgccga cctagctgcg ggcggccgca cgcatggacg cccctccgac
    11041 gtagctgcgg gcagtcgtgc cgctggggcg gcacggtggg cgcagcggca ccccgtcgcg
    11101 ccgggttgcg ccacccaccc ccggcccgca ccagcgctgg gtgcccccgc agtgccggtc
    11161 cacgtgccgc cccggccccc ggcctccgcg tcccacccgc ccaccgtccc cgccctccgg
    11221 ttaggctctg gccaggacga cctgagggaa agggaggccg gacatgacgg cgccggggcg
    11281 caggagcagt accttctctc ggctgctgcg gcacggcttc accgacgcct ccgccgccga
    11341 acgcctcctc gacgcacccg aactcgcccc cgtccgcaac gaccccgtcc tgctggaagc
    11401 actgggcgcc accgccgacc ccgacctcgc cctccgcggc ctcgtcggcc tgctggaggc
    11461 ccagtccgcc cccgcggccc gcagggaact gctcgacacg gtgatagccg ccaagcccct
    11521 gcgcgaccgc ctcctcggcg tcctcggcgc ctccgtcgcg ctcgccgacc acctcgcccg
    11581 ccacccgcag gactggcggg cgctcgtgat gtacgagccg caggatctgc acccgggggt
    11641 ggaggagttc gagcgggggc tcgccgaggc cggcgacccc gtcgccctgc gcgtcgccta
    11701 ccgccgctgc ctgctctccg tcgccgcacg ggacgtctgc ggcaccaccg acgtcgccca
    11761 gaccgccgcc gagctggccg acctcgccac cgccaccctg cgcgccgccc tcgccctggc
    11821 gaaggccgcc gcgcccgagg acgcggcgct gtgccggctc gcggtcatcg cgatgggcaa
    11881 gtgcggcggg cacgagctga actacgtctc cgacgtcgac gtcatcttcg tcggcgaggc
    11941 cgtcgacggc gccgacgagg ggaaggcgct gcgggccgcc acccggctcg cctcgcacat
    12001 gatgcggatc tgttccgaga cgaccgtcga gggctccatc tggcccgtcg acgcgaatct
    12061 gcggcccgag gggcgcaacg ggcccctggt gcggacgctg agcagtcatc tcgcctacta
    12121 ccagcgctgg gccaagacct gggagttcca ggcgctgctc aaggcccggc ccgtcgccgg
    12181 cgacctggag ctgggcgagg agtacgtcgc cgcggtcgaa ccgctggtgt ggaaggccgc
    12241 cgagcgggag aacttcgtcg ccgacgtgca gaagatgcgg cgccgggtcg tggagaacat
    12301 tcccgtcgcc gaggtggagc gggagctgaa gctcgggccc ggcggactgc gggacgtcga
    12361 gttcgccgtg cagctgctgc agctcgtgca cggccgggcc gacgcctcgc tgcgcagtgg
    12421 caagacgctc gacgccttgc aggcgctcgc cgccggcggg tacgtcggcc gcgccgacgc
    12481 cacccagctc gacgaggcct accgcttcct gcgctccatg gagcaccgca tccagctgca
    12541 ccggctgcgc cgcacccacc tcgtccccga ggacgacgcc gacctgcgcc gtatcggacg
    12601 ctcgctcggg ctgcgtaccg acccggtgac cgagctgacc cgcgagtgga agcggcacac
    12661 cagcggcgta cggcggctgc acgagaagct gttctaccgg ccgctgctcg acgccgtggc
    12721 gcagctgccc tccggcgagg tccggctcag cccggaggcg gcacgggagc ggctcgtcgc
    12781 gctcgggtac gccgacccgg cctccgcgct gcgccacctg gaggccctgg cgtccggcgt
    12841 cacccgcaag gccgccatcc agcgcaccct gctgccggtg ctgctcggct ggttcgccga
    12901 ctccgccgac ccggacgccg gactgctcaa cttccgc
//
