LOCUS NZ_BNBR01000010 12937 bp DNA linear CON 02-SEP-2024 DEFINITION Streptomyces griseosporeus strain JCM 4766 sequence10, whole genome shotgun sequence. ACCESSION NZ_BNBR01000010 VERSION NZ_BNBR01000010.1 KEYWORDS WGS; RefSeq; STANDARD_DRAFT. SOURCE Streptomyces griseosporeus ORGANISM Streptomyces griseosporeus Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales; Streptomycetaceae; Streptomyces. REFERENCE 1 AUTHORS Wu,L. and Ma,J. TITLE The Global Catalogue of Microorganisms (GCM) 10K type strain sequencing project: providing services to taxonomists for standard genome sequencing and annotation JOURNAL Int J Syst Evol Microbiol 69 (4), 895-898 (2019) PUBMED 30832757 REFERENCE 2 (bases 1 to 255716) AUTHORS Sun,Q. and Ohkuma,M. TITLE Direct Submission JOURNAL Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL :http://www.wdcm.org/ COMMENT ##Genome-Assembly-Data-START## Assembly Method :: gcType assembly pipeline Genome Coverage :: 112X Sequencing Technology :: Illumina HiSeq X Ten ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_014656055.1-RS_2024_09_02 Annotation Date :: 09/02/2024 05:04:52 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.8 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 7,365 CDSs (total) :: 7,282 Genes (coding) :: 7,186 CDSs (with protein) :: 7,186 Genes (RNA) :: 83 rRNAs :: 2, 4, 2 (5S, 16S, 23S) complete rRNAs :: 2 (5S) partial rRNAs :: 4, 2 (16S, 23S) tRNAs :: 72 ncRNAs :: 3 Pseudo Genes (total) :: 96 CDSs (without protein) :: 96 Pseudo Genes (ambiguous residues) :: 0 of 96 Pseudo Genes (frameshifted) :: 41 of 96 Pseudo Genes (incomplete) :: 61 of 96 Pseudo Genes (internal stop) :: 11 of 96 Pseudo Genes (multiple problems) :: 15 of 96 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 17:46:46 NOTE :: This is a single region extracted from a larger record! Orig. start :: 57457 Orig. end :: 70394 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to BNBR01000010.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ FEATURES Location/Qualifiers region 1..12937 /candidate_cluster_numbers="1" /contig_edge="False" /product="hydrogen-cyanide" /region_number="2" /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)" /tool="antismash" cand_cluster 1..12937 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)" /kind="single" /product="hydrogen-cyanide" /protoclusters="1" /tool="antismash" protocluster 1..12937 /aStool="rule-based-clusters" /category="other" /contig_edge="False" /core_location="[5000:7937](+)" /cutoff="5000" /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)" /neighbourhood="5000" /product="hydrogen-cyanide" /protocluster_number="1" /tool="antismash" proto_core 5001..7937 /aStool="rule-based-clusters" /tool="antismash" /cutoff="5000" /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)" /neighbourhood="5000" /product="hydrogen-cyanide" /protocluster_number="1" gene complement(494..970) /locus_tag="IE308_RS35450" /old_locus_tag="GCM10018783_71710" CDS complement(494..970) /codon_start=1 /gene_functions="biosynthetic-additional (smcogs) SMCOG1298: putative carboxymuconolactone decarboxylase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014139751.1" /locus_tag="IE308_RS35450" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="GCM10018783_71710" /product="carboxymuconolactone decarboxylase family protein" /protein_id="WP_110627792.1" /transl_table=11 /translation="MDARLNLHENAIAAKFFKRLNAAAAVVAEAGLPERTGNLVVLRAS QINGCGFCTDMHTKDALAAGEDSTRLALVAAWREATLFTDAERAALELTEEATRIADAA GGVSDEVWERAARHYDEDQLVALICLIGVINAYNRANVVVRTPAGWYEPGMFAA" misc_feature 1049..1064 /note="TFBS match to CRP, Cyclic AMP receptor protein, confidence: medium, score: 17.4" /tool="antismash" gene complement(1060..2460) /locus_tag="IE308_RS35455" /old_locus_tag="GCM10018783_71720" CDS complement(1060..2460) /EC_number="3.4.11.-" /GO_component="GO:0016020 - membrane [Evidence IEA]" /GO_function="GO:0004177 - aminopeptidase activity [Evidence IEA]" /GO_function="GO:0046872 - metal ion binding [Evidence IEA]" /GO_function="GO:0070006 - metalloaminopeptidase activity [Evidence IEA]" /GO_process="GO:0006508 - proteolysis [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (smcogs) SMCOG1172: methionine aminopeptidase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008743074.1" /locus_tag="IE308_RS35455" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="GCM10018783_71720" /product="aminopeptidase P family protein" /protein_id="WP_190221028.1" /transl_table=11 /translation="MSDTAPPLRTGSHDLSVSTELARFMGRDWAPTPLPQDARVAARAV TPARRARLSARFPGERLVIPAGELKVRSNDCDHRFRPYSAYAWLTGLTGEDQPGHVLVL EPSGPHDHEAVLYIRPRSPRTGRAERPADAFYRDRRHGEFWVGRRPDLAETERLTGIRC ADLDEPGGTLPPGREPSTDPELAAVLSELRLIKDDWEIDQLQQAVDHTTAGFEDVVRSL PRALAHPRGERWIEGVFGLRARAEGNGLGYETIAASGAHACVLHWTRNDGALNPADLLL LDAGVETDTLYTADITRTLPLSGRFSPVQRQVYELVLAAQEAGIAALRPGALFRDFHRA AMRVIAEGLAEWGVLKDAEGDLHRRYTLCSSGHMLGLDVHDCAQARAGAYLDGVLEEGQ VLTVEPGLYLQPDDLTLPPELRGIGVRIEDDLVVTSDGARLLSSALPRTPDAVEEWMGT LLDSTTTQ" misc_feature 2495..2511 /note="TFBS match to ZuR_variant_2, Zinc-responsive repressor, confidence: weak, score: 15.29" /tool="antismash" misc_feature 2499..2509 /note="TFBS match to HypR, L-hydroxyproline utilization repressor, confidence: weak, score: 18.94" /tool="antismash" misc_feature 2503..2516 /note="TFBS match to MogR, Repressor of flagellar motility genes, confidence: weak, score: 12.09" /tool="antismash" gene 2611..4932 /locus_tag="IE308_RS35460" /old_locus_tag="GCM10018783_71730" CDS 2611..4932 /EC_number="3.4.24.3" /GO_component="GO:0005576 - extracellular region [Evidence IEA]" /GO_function="GO:0004222 - metalloendopeptidase activity [Evidence IEA]" /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]" /GO_process="GO:0006508 - proteolysis [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019890144.1" /locus_tag="IE308_RS35460" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="GCM10018783_71730" /product="collagenase" /protein_id="WP_373314676.1" /transl_table=11 /translation="MFHQRTALLAAAVITATATLLGASGPSAAHAVGNGLGPALSVPAG RQPGDDVDHLGKAPARVAPTAQAPGAHRADGRVPGAAAPRTAGARTAAAPRTAVAPCTL DGVTGLAPEPFADFLAAPDVTADGCLRDILWTWDARLAPVMDDAHVQAVARRATALAAR HDGRDSSHLLEMFTYLHAVAYHDFARDEVDISDAPTLEAVRRAVYYFGTAPRTFDATRA NAETLREALYTGSAPGLRQSQLDLIRKVLATLDRHHTSTYADPSWGGAALAALSVNYLG LYEGNHDTAFRTLAARNDAYRDAFRRFAEYTHLKGTPNEWAVRDALSEYARFGTVASLR GRIVADLGALLAPVTDDWGRGSAPWAKVVSWLIHYEACAPYAVCADDIERQLFPQTYRY DNGALTVRTALDRATVDQLYYASKQVKAQFHRVLGARAPLAGDTNTVLTVVLYASRADY ENYHPLLTGMGTNNGGIYIERGATFYTYQRRVPQDSRLTLEELFRHEYVHYLNGRWAVP GYFGEGPWYQGDRTTAMDEGTAEFFAGSTRADGVAVRRSLVEGIIRDTANGGPRMTVDR LLHATYDGDGFRFYDYAGTFFDYLWTEHPSLLREMYRYLRADDPAAFDAWRDRAGRDAA LQQGYDAFLDRQIARVTDLYVPRTSFTPNDGLREATADAVRSAFAAATSADPACAADGD PGMPRFACTGRITARLTDAGSADLVFRDMTETVDAYLLDRAAPASPNLADMTCSFGEVT IWSDRTGGTADYTCEGPLRR" gene 5001..6233 /locus_tag="IE308_RS35465" /old_locus_tag="GCM10018783_71740" CDS 5001..6233 /EC_number="1.-.-.-" /GO_function="GO:0016491 - oxidoreductase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) hydrogen-cyanide: DAO" /gene_functions="biosynthetic-additional (smcogs) SMCOG1103: FAD dependent oxidoreductase" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018850110.1" /locus_tag="IE308_RS35465" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="GCM10018783_71740" /product="FAD-binding oxidoreductase" /protein_id="WP_190221145.1" /sec_met_domain="DAO (E-value: 2.7e-72, bitscore: 238.1, seeds: 521, tool: rule-based-clusters)" /transl_table=11 /translation="MSAPSLDAVIVGAGVVGAACAYYATRAGLSVAVVDRGPVAGGTTG AGEGNLLVSDKEPGPELELALLSTRLWRELAAALPPEDAVEYEPKGGLVLAPDETALTA LRAFAAGQCAAGVEAREVDEHELAHLEPELTKGLAGGFHYPQDAQVQPARAAAALLRAA RSRGALVRLGEEVTAVLTGPHGAVRGVRTPRGELHAPAVVNATGTWGGRLAALAGTRLP VLPRRGFVLVTEPLPPGLIRHKVYAADYIADVASDSAALQSSAVVEGTPAGPVLIGATR ERVGFDRTLSTEALRRLAGQATALFPRLADVHAIRTYCGFRPYLPDHLPAIGPDPRVPG LHHACGHEGAGIGLAPATGLLVTAALTGDEPPVGAWPFRPERFDSRGPADDFSDPFDRP DGPPPSPGART" gene 6230..6541 /locus_tag="IE308_RS35470" /old_locus_tag="GCM10018783_71750" CDS 6230..6541 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) hydrogen-cyanide: Fer2_4" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015038322.1" /locus_tag="IE308_RS35470" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="GCM10018783_71750" /product="(2Fe-2S)-binding protein" /protein_id="WP_190221030.1" /sec_met_domain="Fer2_4 (E-value: 1.1e-26, bitscore: 86.6, seeds: 114, tool: rule-based-clusters)" /transl_table=11 /translation="MSQTPLGLVRAAPAAPYTFTFDGRILTALPGQTLAAALWAAGIMS WRTTRGGGAPRGVFCGIGVCHDCLATVNGRPNQRACLVAVQPGDVVTVQEGTGHDATR" gene 6525..7937 /locus_tag="IE308_RS35475" /old_locus_tag="GCM10018783_71760" CDS 6525..7937 /GO_function="GO:0016491 - oxidoreductase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) hydrogen-cyanide: Pyr_redox_2" /gene_functions="biosynthetic (rule-based-clusters) hydrogen-cyanide: Fer2_BFD" /gene_functions="biosynthetic-additional (smcogs) SMCOG1176: alkyl hydroperoxide reductase subunit" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017237884.1" /locus_tag="IE308_RS35475" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="GCM10018783_71760" /product="NAD(P)/FAD-dependent oxidoreductase" /protein_id="WP_190221031.1" /sec_met_domain="Pyr_redox_2 (E-value: 3.8e-27, bitscore: 89.1, seeds: 64, tool: rule-based-clusters)" /sec_met_domain="Fer2_BFD (E-value: 1.3e-12, bitscore: 41.8, seeds: 211, tool: rule-based-clusters)" /transl_table=11 /translation="MTPPVDLAVVGAGPAGLAAAVRAAGLGLSVALLDAGERPGGQFHR YPAPGLRAARPEALHHGWRHFARRLRQLETGGVTYLPRHHVWTVARGDGCWLLHAVVGP DETPATVRARAVLLATGAYERQLPFPGWTLPGVVGAGGAQAMLKSGLVLPGRRVVVAGS GPLLLAVAASLAAAGARVPAVVEAADYRAYGRSLPAVLRTPAKLAEGARYGGGLLRRST RLLTRHAVTAAHGTARVEAVTVTRLDDHWRPVPGTGRRVPCDALAVGHGLVPQLDLAVG LGCATRRTADDTHALVVDADQATTVPGIWAAGESCGIGGADLALTEGEIAADAVARTLH DVPADPRRHAALTRRRTRLRAFADAMAAAHRPGPGWADWLDDATEVCRCEEVTAGRVRE AVAGYGAHDTRTVKLLTRAGMGWCQGRMCGQAVARLAGGEGAAAVESRPLSCPVPLRPL AQLPPEAEEFRA" gene 7934..8878 /locus_tag="IE308_RS35480" /old_locus_tag="GCM10018783_71770" CDS 7934..8878 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018543026.1" /locus_tag="IE308_RS35480" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="GCM10018783_71770" /product="dihydrodipicolinate synthase family protein" /protein_id="WP_190221032.1" /transl_table=11 /translation="MTVPDTRPRPWHGVMVATALPLRPDLGVDYDAYAAHVARLVDAGC DGVVPNGSLGEYQTLTDDERARVVRVAVEAAGDGARVMPGVSAYGGAEARRWAEQAAEA GCGSVLLLPPNAYRADERAVRAHYAEVARAGLPVVAYNNPYDTKVDLTPELLARLHGDG HIVAVKEFSGDVRRAYEIAELAPELDLLIGADDVLLELALAGAVGWIAGYPNALPHACV DLYRAAVARDLERALPLYRALHPLLRWDSKTEFVQAIKLSMDLAGLPGGPTRPPRGPLG EAEAAAVRAATEKVLAEGFGEASGLSGLSGEVR" gene 8878..9882 /locus_tag="IE308_RS35485" /old_locus_tag="GCM10018783_71780" CDS 8878..9882 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019891275.1" /locus_tag="IE308_RS35485" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="GCM10018783_71780" /product="proline racemase family protein" /protein_id="WP_190221033.1" /transl_table=11 /translation="MRTRHVYHAVDSHTEGMPTRVVTGGVGVLPGATMAERRRYLVEHL DHVRTLLMYEPRGHAAMSGAILQPPTRPDADWGVLFIEVSGALPMCGHGTIGVATVLVE TGMVPVVEPVTTVRLDTPAGLVSVDVRVADGVAESVTLTNVPAYCAGLGRTVEVPGYGT VAYDLAYGGNFYAFVELDALGLPYERERKGELLAAGLAIMDAVNGGADRPVHPENPDIA GLKHVYLIAPGSDAKHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLGADFVNE SFIGTRFVGRLIGDAAVGGVPAVVPAVTGRAWITGTAQYFLDPEDPFPAGFLL" gene 9964..10632 /locus_tag="IE308_RS35490" /old_locus_tag="GCM10018783_71790" CDS 9964..10632 /GO_function="GO:0003700 - DNA-binding transcription factor activity [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1071: GntR family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004938955.1" /locus_tag="IE308_RS35490" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="GCM10018783_71790" /product="GntR family transcriptional regulator" /protein_id="WP_190221034.1" /transl_table=11 /translation="MGGHLKQANLITAQERLRDQVAHALRAALISGELQPGRVYSAPAL ASDFGISATPVREAMLDLVREGLVEPVRNKGFRVTEVSEKDLDQYSELRALIEVPTVGA VTRSASREQLEALRPVAEEIVATAREHDLIGYLDADRRFHLSLLALHGNDRLVETVGDL RKRSRLYGLTALDERGLLVASAEEHLELLERMLAGDGEGAEAVMRRHLGHVRALWAAES " misc_feature 10789..10805 /note="TFBS match to HexR, Glucose-responsive regulator, confidence: weak, score: 15.88" /tool="antismash" misc_feature complement(11221..11234) /note="TFBS match to CatR, H2O2-responsive repressor, confidence: medium, score: 18.39" /tool="antismash" ORIGIN 1 tccgtggcgc gcctgcggta ctcctggacc gcccagccgt tgccgtcggg gtccttgaag 61 tacaggaagg tggcgccgtc gtccggggcg taactcaccg gctcggagac gtccaggccc 121 cgctcggtca gctccgcgtg ggcggccttg atgtcggcca cgcacagctg gaggccctgg 181 taggagccgg gggccgggcg cgggccgccg gccatctccc agatgctgtc gccgagcgcg 241 atcgaacagc cggagcccgg gggcgtcagc tggacgatcc gcatccccgg catgacctcc 301 gtgtcgatgt cgacgtggaa gccgaccttg tcgcggtaga agtcccgggc ccggtcgatg 361 tcgctcaccg gcagggggat cacttcgagg gtgaagtcca tcgttccgcc tcctggaggc 421 tcgtggtggt ccccttcacc gaagcggaaa accgggcccg gcgccaccgc atcggcctgc 481 cggccgcgtg gatctacgcc gcgaacatgc ccggctcgta ccagccggcc ggtgtgcgca 541 cgacgacgtt cgcccggttg taggcgttga tcacgccgat caggcagatc agcgccacca 601 gctggtcctc gtcgtagtgc cgggcggccc gctcccagac ctcgtcggac accccgcccg 661 ccgcgtcggc gatccgggtg gcctcctcgg tcagctccag ggccgcgcgc tcggcgtcgg 721 tgaacagcgt cgcctcccgc caggccgcga ccagggcgag gcgggtggag tcctcgccgg 781 ccgcgagcgc gtccttggtg tgcatgtcgg tgcagaaacc gcagccgttg atctggctgg 841 cgcggagcac caccaggttc ccggtgcgct ccggcaggcc ggcctcggcg accaccgccg 901 cggcggcgtt gaggcgcttg aagaacttgg cggcgatcgc gttctcgtgc aggttcagtc 961 gggcgtccat cgcggtgttc ctccgtcgtc gtgcgagtgg gtacgccggt gtgacggagg 1021 aacccgacgc gatgtgacgg ccgagcgatg tgacctgggt cactgtgtgg tggtgctgtc 1081 gaggagcgtc cccatccact cctccaccgc gtccggtgtg cgcggcagcg ccgaggacag 1141 cagccgtgcg ccgtcggagg tgacgaccag gtcgtcctcg atccgcacgc cgatgccgcg 1201 cagctcgggc ggcagggtga ggtcgtcggg ctggaggtag aggccgggtt cgacggtgag 1261 cacctggccc tcctccagga cgccgtccag ataggcgccg gcccgcgcct gggcgcagtc 1321 gtgcacgtcc aggccgagca tgtgcccgct gctgcacagg gtgtagcgcc ggtgcagatc 1381 gccctcggcg tccttcagga cgccccactc ggccagcccc tccgcgatga cccgcatcgc 1441 ggcccggtgg aagtcgcgga agagcgcgcc gggccgcagc gccgcgatac cggcctcctg 1501 cgcggcgagg accagctcgt acacctggcg ctggacgggg gagaagcggc cggagagggg 1561 gagcgtgcgc gtgatgtcgg cggtgtagag ggtgtcggtc tccacgcccg cgtcgagcag 1621 cagcaggtcg gccgggttca gcgcgccgtc gttgcgggtc cagtgcagca cgcacgcgtg 1681 ggcgccggac gccgcgatcg tctcgtaccc gaggccgttg ccctcggcgc gggcgcgcag 1741 cccgaagacg ccctcgatcc agcgttcgcc gcgcgggtgg gcgagggcgc gcggcaggga 1801 ccgtacgacg tcctcgaagc cggccgtcgt gtggtcgacg gcctgctgca gctggtcgat 1861 ctcccagtcg tccttgatga ggcgcaactc cgacagtacg gcggccagtt cggggtccgt 1921 ggacggttcg cggcccggcg gcagggtgcc gcccggctcg tcgaggtcgg cgcagcggat 1981 accggtcagg cgctcggtct cggccaggtc ggggcggcgg ccgacccaga actcgccgtg 2041 gcggcggtcc cggtagaagg cgtcggcggg ccgctcggcc cggccggtgc gcggcgagcg 2101 ggggcgtatg tacagcaccg cctcgtggtc gtgcgggccc gacggctcca ggaccaggac 2161 gtggccgggc tggtcctcgc cggtgaggcc ggtcagccag gcgtaggcgc tgtacggccg 2221 gaagcggtgg tcgcagtcgt tggagcggac cttcaactcg ccggcgggga tcaccagtcg 2281 ctcgccgggg aaccgcgccg acagccgggc ccggcgggcc ggggtgaccg cgcgggcggc 2341 gacgcgggcg tcctgcggca gcggtgtcgg cgcccagtcg cggcccatga aacgggccaa 2401 ctccgtggac acggacaggt cgtggctgcc ggtgcgcagg ggcggggcgg tgtcggacat 2461 gacggctcct gaagcgagga ctcctgaggg gttgtcagtg caatttcaca ttactatgtt 2521 acagctcaca tgacagtggc atggacgcat caagctgatc gctgtcggtc cgtgccctgg 2581 tcgagccact ccccacacga ggagccgctg gtgtttcatc aacgcaccgc cctgctcgcc 2641 gcggcggtca tcaccgcgac cgcgaccctg ctcggcgcgt cgggcccgtc cgccgcgcac 2701 gccgtcggca acggcctcgg tcctgccctc tccgtccccg ccggccgcca accgggcgac 2761 gacgtcgacc acttgggcaa ggcgccggcc cgcgtcgccc ccaccgccca ggcccccggc 2821 gcacaccgcg ccgacggccg cgtccccggc gccgccgccc cgcggaccgc cggtgcccgc 2881 acggccgccg ccccgcggac agccgtcgcc ccctgcaccc tcgatggcgt caccggtctc 2941 gcccccgagc cgttcgccga cttcctggcc gccccggacg tcaccgccga cggttgcctg 3001 cgcgacatcc tgtggacctg ggacgcgcgc ctcgcgcccg tcatggacga cgcccacgtg 3061 caggccgtcg cccgccgcgc caccgcgctc gccgcccgcc acgacggccg cgactccagc 3121 cacctactgg agatgttcac ctacctgcac gccgtcgcct accacgactt cgcgcgcgac 3181 gaggtcgaca tcagcgacgc gcccaccctg gaggccgtgc gccgcgccgt gtactacttc 3241 ggcaccgcgc cccgcacctt cgacgccacc cgcgccaacg ccgagaccct ccgcgaggcc 3301 ctgtacaccg gcagcgcgcc cggcctgcgc cagtcccagc tcgacctgat ccgcaaggtg 3361 ctggccaccc tcgaccgcca ccacacgagc acctacgccg acccgtcctg gggcggcgcc 3421 gcgctcgccg cgctcagcgt caactacctc ggcttgtacg agggcaacca cgacaccgcc 3481 ttccgcaccc tcgccgcccg caacgacgcc taccgcgacg ccttccgccg cttcgccgag 3541 tacacccacc tcaagggcac gcccaacgag tgggccgtcc gcgacgccct gtccgagtac 3601 gcccgcttcg gcaccgtcgc ctccctgcgc ggccggatcg tcgccgacct cggcgccctc 3661 ctcgccccgg ttacggacga ctggggccgg ggcagcgccc cctgggccaa ggtcgtctcc 3721 tggctgatcc actacgaggc ctgcgcgccg tacgccgtct gcgcggacga catcgagcgg 3781 cagctgttcc cgcagaccta ccgctacgac aacggcgccc tcaccgtccg caccgccctc 3841 gaccgcgcca ccgtcgacca gctctactac gcgagcaagc aggtcaaggc gcagttccac 3901 cgcgtcctcg gcgcccgggc acccctggcc ggcgacacca acaccgtgct caccgtcgtg 3961 ctgtacgcct cccgcgccga ctacgagaac taccacccgc tgctcaccgg catgggcacg 4021 aacaacggcg gcatctacat cgagcgcggc gccaccttct acacctacca gcggcgcgtc 4081 ccgcaggact cccggctgac gctggaggag ctgttccgcc acgagtacgt gcactacctc 4141 aacggccgct gggccgtgcc cggttacttc ggcgagggcc cctggtacca gggcgaccgc 4201 accaccgcca tggacgaggg caccgcggag ttcttcgccg gctccacccg cgccgacggc 4261 gtcgccgtcc gcagatccct ggtggagggg atcatccggg acaccgccaa cggcggaccg 4321 cgcatgaccg tcgaccggct cctgcacgcc acctacgacg gcgacggctt ccgcttctac 4381 gactacgccg gcacgttctt cgattacctc tggaccgaac acccctccct gctgcgggag 4441 atgtaccgct acctgcgggc ggacgacccc gccgccttcg acgcctggcg ggaccgcgcg 4501 ggccgcgacg ccgccctcca gcagggctac gacgccttcc tggaccggca gatcgcccgc 4561 gtcaccgacc tgtacgtgcc ccgcacgtcc ttcaccccga acgacgggct gcgcgaggcc 4621 accgccgacg ccgtgcgctc cgccttcgcc gccgccacct ccgccgaccc cgcgtgcgcg 4681 gccgacggcg accccggcat gccccgcttc gcgtgcaccg gtcgcatcac cgcccggctc 4741 accgacgcgg gcagcgccga cctggtgttc cgggacatga cggagaccgt cgacgcctac 4801 ctgctcgacc gggccgcgcc cgcctcgccc aacctcgccg acatgacctg ctccttcggg 4861 gaggtgacga tctggtccga ccgcacgggc gggacggccg actacacctg cgaggggccg 4921 ctgcggcggt gaccgggacg cgggcacggg ctcttgtgag gcacagtgcc atgtgaaata 4981 ttactgacgt gcccacgaag atgtccgcac cctctctcga cgccgtgatc gtcggcgccg 5041 gagtggtcgg cgcggcgtgc gcgtactacg ccacccgcgc cggcctctcc gtcgcggtcg 5101 tcgaccgcgg ccccgtcgcc ggcggcacca ccggagccgg cgagggaaac ctcctcgtct 5161 ccgacaagga gccgggaccc gaactcgaac tcgccctgct gtccacgcgg ttgtggcggg 5221 aactggccgc ggccctcccg cccgaggacg ccgtcgagta cgaacccaag ggcggcctcg 5281 tcctcgcccc cgacgagacc gccctgaccg cgctccgcgc cttcgcggcc ggacaatgcg 5341 ccgccggagt cgaggcgcgg gaagtcgacg agcacgaact ggcccacctc gaaccggagt 5401 tgaccaaggg actcgccggc ggcttccact acccgcagga cgcccaggtc cagcccgccc 5461 gggccgccgc cgccctgctg cgcgccgccc ggagccgcgg cgccctcgtc cggctcggcg 5521 aggaggtcac cgccgtcctc accggcccgc acggcgccgt ccgcggcgtg cgcaccccgc 5581 gcggcgagct gcacgccccc gccgtcgtca acgccaccgg cacctggggc ggccggctcg 5641 ccgccctggc cggcacccgc ctgccggtcc tgccgcggcg cggcttcgtc ctcgtcaccg 5701 aacccctgcc gcccggactg atccggcaca aggtgtacgc cgccgactac atcgcggacg 5761 tcgccagcga ctccgccgct ctccagtcct ccgccgtcgt cgagggcacc cccgccggac 5821 ccgtcctcat cggcgccacc cgcgaacgcg tcggcttcga ccgcaccctg tccaccgagg 5881 ccctgcgccg cctcgccggc caggccaccg ccctcttccc ccgcctcgcc gacgtccacg 5941 cgatccgcac gtactgcggc ttccgcccct atctgcccga ccacctgccc gcgatcgggc 6001 ccgacccccg cgtccccggc ctccaccacg cctgcggcca cgaaggcgcc ggcatcggcc 6061 tcgccccggc cacgggactg ctggtgacag cggccctgac cggggacgaa ccgccggtcg 6121 gggcctggcc gttccggcct gagcgattcg acagccgggg ccccgccgac gacttctccg 6181 accccttcga ccgtcccgac ggccccccgc cctccccagg agcccgcaca tgagccagac 6241 cccgctcggc ctggtgcgcg ccgcgccggc agcgccgtac accttcacct tcgacggacg 6301 catcctcacc gccctgcccg gccagaccct ggccgccgcg ctgtgggccg ccgggatcat 6361 gtcctggcgc accacccgcg gcggcggcgc cccgcgcggg gtgttctgcg ggatcggcgt 6421 ctgccacgac tgcctcgcca ccgtcaacgg ccgccccaac cagcgcgcct gcctggtcgc 6481 cgtgcagccc ggggacgtcg tcaccgtgca ggaggggacc gggcatgacg ccacccgttg 6541 acctcgccgt cgtcggcgcc ggaccggccg gcctcgccgc cgccgtccgc gccgccggcc 6601 tcgggctgag cgtggcgctg ctggacgcgg gggagcggcc cggcggccag ttccaccggt 6661 accccgcccc cggcctgcgc gccgctcggc ccgaggccct gcaccacggc tggcggcact 6721 tcgcccggcg gctgcggcaa ctggagacgg gtggtgtgac gtacctgccc cgccaccacg 6781 tgtggaccgt cgcccgcggg gacggctgct ggctgctgca cgccgtcgtc ggccccgacg 6841 agacgcccgc cacggtccgc gcccgtgccg tcctcctcgc caccggcgcc tacgagcgcc 6901 agctcccctt ccccggctgg accctgcccg gagtcgtcgg cgcgggcggc gcccaggcca 6961 tgctcaagtc cgggctcgtg ctgcccggcc ggcgggtcgt cgtcgccggc agcggcccgc 7021 tcctgctcgc cgtcgccgcg tcgctcgccg ccgccggggc ccgcgtcccc gccgtcgtcg 7081 aggccgccga ctaccgcgcg tacggccgca gcctgccggc cgtgctgcgc acccccgcca 7141 aactcgccga gggcgcccgc tacggcggcg gtctcctgcg ccgctccacc cggctgctca 7201 cccggcacgc cgtcaccgcc gcccacggca ccgcccgcgt cgaggccgtc accgtcaccc 7261 gcctcgacga ccactggcgg cccgtgcccg gcaccggccg ccgcgtgccc tgcgacgcgc 7321 tcgccgtcgg ccacggcctc gtcccgcagc tcgacctcgc cgtcggcctc ggctgcgcca 7381 cccgccgcac cgccgacgac acccacgccc tcgtcgtcga cgccgaccag gcgaccaccg 7441 tgccgggcat ctgggccgcc ggcgagtcct gcggcatcgg cggcgccgac ctcgcgctca 7501 ccgagggcga gatcgccgcc gacgccgtcg cccgcaccct gcacgacgtc ccggccgacc 7561 cgcgccgcca cgccgccctc acccgccgcc ggacccggct gcgcgccttc gccgacgcca 7621 tggccgccgc ccaccggccc ggccccggct gggccgactg gctcgacgac gccaccgagg 7681 tgtgccgctg cgaggaggtc accgccggac gcgtccggga ggcggtcgcc gggtacggcg 7741 cccacgacac ccgcaccgtc aagctgctca cccgcgccgg catgggctgg tgccagggcc 7801 gcatgtgcgg ccaggccgtc gcccggctcg ccgggggaga gggggccgcc gccgtggaga 7861 gccggccgct gtcctgtccc gtaccgctgc gccccctcgc ccaactgccg cccgaagccg 7921 aggagttccg agcatgaccg ttcccgacac ccgcccccgc ccctggcacg gcgtcatggt 7981 cgccaccgcc ctgccgctgc gccccgacct cggcgtcgac tacgacgcct acgccgccca 8041 cgtcgcccgc ctcgtcgacg ccggctgcga cggcgtcgtc cccaacggct ccctcggcga 8101 gtaccagacc ctcaccgacg acgaacgcgc ccgcgtcgtg cgcgtcgccg tcgaggccgc 8161 aggcgacggc gcccgcgtca tgcccggcgt gtccgcgtac ggcggcgccg aggcccgccg 8221 ctgggccgag caggccgccg aggccggctg cgggtccgtg ctgctgctgc cgcccaacgc 8281 ctaccgggcc gacgagaggg cggtacgggc ccactacgcc gaggtggcgc gggccggcct 8341 gccggtcgtc gcgtacaaca acccgtacga caccaaggtc gacctcacgc cggagctgct 8401 cgcccggctg cacggcgacg ggcacatcgt cgccgtcaag gagttcagcg gcgacgtccg 8461 ccgcgcctat gagatcgccg aactcgcgcc cgagctcgac ctgttgatcg gcgccgacga 8521 cgtgctgctg gaactcgccc tcgccggggc cgtcggctgg atcgccggct accccaacgc 8581 gctcccgcac gcgtgcgtgg acctctaccg ggctgcggtc gcccgcgacc tggagcgggc 8641 gctgccgctg taccgggcgc tgcacccgct gctgcgctgg gactcgaaga ccgagttcgt 8701 gcaggccatc aagttgtcca tggacctcgc cgggctgccc ggcggaccca cccggccgcc 8761 gcggggcccg ctgggggagg cggaggcggc ggctgtgcgc gccgccacgg agaaggtgct 8821 ggcggagggg ttcggtgagg cttccggcct ctcgggtctc tcgggggagg tgcggtaatg 8881 cggacccggc acgtctacca cgctgtcgac tcgcacaccg agggcatgcc cacgcgggtc 8941 gtcaccggag gagtcggggt gctgcccggg gcgactatgg ccgagcgccg gcggtacctg 9001 gtcgagcatc tcgaccacgt ccgcaccctg ttgatgtacg agccgcgcgg ccacgccgcc 9061 atgagcggcg cgatcctcca gccgccgacc cggcccgacg ccgactgggg cgtcctgttc 9121 atcgaggtgt ccggcgcgct gccgatgtgc gggcacggca cgatcggcgt cgccaccgtg 9181 ctcgtggaga ccgggatggt gcccgtcgtc gaaccggtca ccacggtacg tctggacacc 9241 ccggccgggc tggtctcggt ggacgtccgg gtcgcggacg gcgtggccga gtcggtcacg 9301 ctgacgaacg tgccggcgta ctgcgccggg ctggggcgga cggtggaggt gccggggtac 9361 ggcaccgtcg cctacgacct cgcctacggc gggaatttct acgccttcgt ggaactggac 9421 gcgctcgggc tgccgtacga gcgggagcgc aagggggagc tgctggcggc cgggcttgcc 9481 atcatggacg cggtgaacgg cggggccgac cggcccgtcc acccggagaa cccggacatc 9541 gccgggctca agcacgtgta cctgatcgcg cccggctccg acgccaagca ctcccggcac 9601 gccatggcga tccaccccgg gtggttcgac cggtcgccgt gcggcacggg gacgtcggcg 9661 cggatggcgc agctgcacgc gcgcggtgag ctgccgctcg gggcggactt cgtgaacgag 9721 tcgttcatcg gtacgcggtt cgtggggcgg ttgatcggcg acgcggcggt cgggggtgtg 9781 ccggcggtgg tgccggcggt caccgggcgg gcctggatca cggggaccgc gcagtacttc 9841 ctggacccgg aggatccttt cccggcgggt ttcctactct gaggtgcccg agagcccgag 9901 agcccgagag cccgcgagac ccgtaccgag ccgagacctg caaacccccc ggaggcagac 9961 accatgggtg gccacctgaa gcaagccaac ctcatcaccg cgcaggagcg gctgcgcgac 10021 caggtggccc atgccctgcg cgccgcgctg atctccgggg agctgcagcc ggggcgggtg 10081 tactcggcgc cggcgctcgc ctcggacttc ggcatctcgg cgacgcccgt gcgggaggcg 10141 atgctcgacc tggtgcggga ggggctggtc gagcccgtcc ggaacaaggg gttccgggtg 10201 accgaggtga gcgagaagga cctcgaccag tacagcgagc tgcgggcgct gatcgaggtg 10261 ccgacggtgg gggcggtcac gcggtcggcg agccgggagc agctggaggc gctgcggccg 10321 gtggcggagg agatcgtggc gacggcgcgg gagcacgacc tgatcgggta cctggacgcc 10381 gaccgccgct tccacctgtc cctgctggcg ctgcacggca acgaccggct ggtggagacg 10441 gtgggggacc tgcgcaagcg gtcccggctg tacgggctga cggcgctgga cgagcggggg 10501 ttgctggtcg cgtccgcgga ggagcacctg gagctgctgg agcggatgct ggcgggggac 10561 ggggaggggg cggaggcggt catgcgacgc catctggggc atgtgcgggc gctgtgggcg 10621 gcggagtcgt gaggcgtctg ggggtgggtg gttgctcgcg cagttccccg cgcccctgaa 10681 tgcctgcggc ggcccgtttc tgtccgtggg tggttgctgt agcgcctgcg gctggtggtg 10741 gggtcggggc cgcgccgggg ggtatccgtc ctcggtgcgc cgctgcgtcg gtcaaattct 10801 taccgccact ttccgccgcc cgctgcgggc ggacaccccc cgacacgtcc cctgctcgcc 10861 gtacgcgtct accggccgcc gtgactacgg cagcgccgcc ccgcttgggt agcgggtagc 10921 cggcgccgcc tcgcgacttg gctgcgggca gccgccccgc tgggccggcg cccaccccgc 10981 ggcggcgggc cgcacgccga cctagctgcg ggcggccgca cgcatggacg cccctccgac 11041 gtagctgcgg gcagtcgtgc cgctggggcg gcacggtggg cgcagcggca ccccgtcgcg 11101 ccgggttgcg ccacccaccc ccggcccgca ccagcgctgg gtgcccccgc agtgccggtc 11161 cacgtgccgc cccggccccc ggcctccgcg tcccacccgc ccaccgtccc cgccctccgg 11221 ttaggctctg gccaggacga cctgagggaa agggaggccg gacatgacgg cgccggggcg 11281 caggagcagt accttctctc ggctgctgcg gcacggcttc accgacgcct ccgccgccga 11341 acgcctcctc gacgcacccg aactcgcccc cgtccgcaac gaccccgtcc tgctggaagc 11401 actgggcgcc accgccgacc ccgacctcgc cctccgcggc ctcgtcggcc tgctggaggc 11461 ccagtccgcc cccgcggccc gcagggaact gctcgacacg gtgatagccg ccaagcccct 11521 gcgcgaccgc ctcctcggcg tcctcggcgc ctccgtcgcg ctcgccgacc acctcgcccg 11581 ccacccgcag gactggcggg cgctcgtgat gtacgagccg caggatctgc acccgggggt 11641 ggaggagttc gagcgggggc tcgccgaggc cggcgacccc gtcgccctgc gcgtcgccta 11701 ccgccgctgc ctgctctccg tcgccgcacg ggacgtctgc ggcaccaccg acgtcgccca 11761 gaccgccgcc gagctggccg acctcgccac cgccaccctg cgcgccgccc tcgccctggc 11821 gaaggccgcc gcgcccgagg acgcggcgct gtgccggctc gcggtcatcg cgatgggcaa 11881 gtgcggcggg cacgagctga actacgtctc cgacgtcgac gtcatcttcg tcggcgaggc 11941 cgtcgacggc gccgacgagg ggaaggcgct gcgggccgcc acccggctcg cctcgcacat 12001 gatgcggatc tgttccgaga cgaccgtcga gggctccatc tggcccgtcg acgcgaatct 12061 gcggcccgag gggcgcaacg ggcccctggt gcggacgctg agcagtcatc tcgcctacta 12121 ccagcgctgg gccaagacct gggagttcca ggcgctgctc aaggcccggc ccgtcgccgg 12181 cgacctggag ctgggcgagg agtacgtcgc cgcggtcgaa ccgctggtgt ggaaggccgc 12241 cgagcgggag aacttcgtcg ccgacgtgca gaagatgcgg cgccgggtcg tggagaacat 12301 tcccgtcgcc gaggtggagc gggagctgaa gctcgggccc ggcggactgc gggacgtcga 12361 gttcgccgtg cagctgctgc agctcgtgca cggccgggcc gacgcctcgc tgcgcagtgg 12421 caagacgctc gacgccttgc aggcgctcgc cgccggcggg tacgtcggcc gcgccgacgc 12481 cacccagctc gacgaggcct accgcttcct gcgctccatg gagcaccgca tccagctgca 12541 ccggctgcgc cgcacccacc tcgtccccga ggacgacgcc gacctgcgcc gtatcggacg 12601 ctcgctcggg ctgcgtaccg acccggtgac cgagctgacc cgcgagtgga agcggcacac 12661 cagcggcgta cggcggctgc acgagaagct gttctaccgg ccgctgctcg acgccgtggc 12721 gcagctgccc tccggcgagg tccggctcag cccggaggcg gcacgggagc ggctcgtcgc 12781 gctcgggtac gccgacccgg cctccgcgct gcgccacctg gaggccctgg cgtccggcgt 12841 cacccgcaag gccgccatcc agcgcaccct gctgccggtg ctgctcggct ggttcgccga 12901 ctccgccgac ccggacgccg gactgctcaa cttccgc //