LOCUS       NZ_BNBR01000009        10405 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces griseosporeus strain JCM 4766 sequence09, whole genome
            shotgun sequence.
ACCESSION   NZ_BNBR01000009
VERSION     NZ_BNBR01000009.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces griseosporeus
  ORGANISM  Streptomyces griseosporeus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 394651)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 112X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014656055.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 05:04:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,365
            CDSs (total)                      :: 7,282
            Genes (coding)                    :: 7,186
            CDSs (with protein)               :: 7,186
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 61 of 96
            Pseudo Genes (internal stop)      :: 11 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:46:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 84678
            Orig. end                         :: 95083
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNBR01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    86..100
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.71"
                     /tool="antismash"
     misc_feature    complement(200..214)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 20.18"
                     /tool="antismash"
     gene            complement(584..1444)
                     /locus_tag="IE308_RS33780"
                     /old_locus_tag="GCM10018783_68360"
     CDS             complement(584..1444)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130459.1"
                     /locus_tag="IE308_RS33780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68360"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_110627281.1"
                     /transl_table=11
                     /translation="MNTHPGRRTRRLLAAGTAAAALALVAACTSTDDGGSGSKTAAGGV
                     ELVKAGQLTTCTHLPYPPFQSEIDGKVQGFDVALIDLVAKNLGVKQKIVDTPFENFKTG
                     AFLNSGQCDLAAAGMTITEERKKNVDFSDPYFDATQAVLVDKKSGVSSLADVKSKGVKL
                     GAQAQTTGEDYAKKQGFDPVSFESSDAVLNGLRTGQVKAVIIDYPVVQGWLKDKANADA
                     FKVVDNLNTGEQYGFTVKKGNTKLREAINKAIADAKADGTYKKLYEKWIGPYDASAASP
                     SASAS"
     gene            1673..2773
                     /locus_tag="IE308_RS33785"
                     /old_locus_tag="GCM10018783_68370"
     CDS             1673..2773
                     /NRPS_PKS="Domain: Aminotran_5 (74-311). E-value: 5.3e-30.
                     Score: 96.5. Matches aSDomain:
                     nrpspksdomains_IE308_RS33785_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="IE308_RS33785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68370"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_190220809.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.2e-24, bitscore:
                     80.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAARFASIEDRVARLLHTGQDVLIMQGEALLPLE
                     GAIRAAAGPGTTALNVITGPYGQTFGNWLRDCGAEVIDLAVPFHTAVTAEQIRAALAER
                     PEIDFVSLVHAEAATGNTNPVAEIGPVVREHGALFYLDAVASVGAEPVLVDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPRAPRRSYLSLLDWKERWIDAGRTALPHAP
                     AQLEMLALEACLERIEAQGLDAVMARHARAAAATRAGALALGGGLEPYVHDAADAAPVA
                     TTLRAPAGTDAVELVARALESDPALPVSAGGGALAKEMIRVNHYGPDATAATVRGALVA
                     LGQALAERGLSVDVAAALGAADAAGR"
     aSDomain        1895..2605
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE308_RS33785_Aminotran_5.1"
                     /evalue="5.30E-30"
                     /label="IE308_RS33785_Aminotran_5.1"
                     /locus_tag="IE308_RS33785"
                     /protein_end="311"
                     /protein_start="74"
                     /score="96.5"
                     /tool="antismash"
                     /translation="RDCGAEVIDLAVPFHTAVTAEQIRAALAERPEIDFVSLVHAEAAT
                     GNTNPVAEIGPVVREHGALFYLDAVASVGAEPVLVDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWARMAANPRAPRRSYLSLLDWKERWIDAGRTALPHAPAQLEMLALEACLERI
                     EAQGLDAVMARHARAAAATRAGALALGGGLEPYVHDAADAAPVATTLRAPAGTDAVELV
                     ARALESDPALPVSAG"
     misc_feature    2986..3001
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    3121..3133
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     gene            3158..3664
                     /gene="ectA"
                     /locus_tag="IE308_RS33790"
                     /old_locus_tag="GCM10018783_68380"
     CDS             3158..3664
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002246.1"
                     /locus_tag="IE308_RS33790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68380"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_110627283.1"
                     /transl_table=11
                     /translation="MTAAPADLLIDRPSVADGSALWRIARDSEALDLNSSYSYLLWCRD
                     FAGTSAVARREDGQPIGFVTGYVRPERPHTLLVWQVAVDSAYRGRGLAAALLDGLTARL
                     AAERAITEVETTITPGNTASERLFTSFAARHGATVEREVLFDAGLFPDGPHDPEVLYRI
                     GPLSL"
     gene            3708..4979
                     /gene="ectB"
                     /locus_tag="IE308_RS33795"
                     /old_locus_tag="GCM10018783_68390"
     CDS             3708..4979
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.5e-74.
                     Score: 243.0. Matches aSDomain:
                     nrpspksdomains_IE308_RS33795_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="IE308_RS33795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68390"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_110627284.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.5e-79, bitscore:
                     260.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLMRDGVTHGLDMSTTAKRAFLESFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGKVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALKELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATAALETYWTDGSAMEKQTRTRGEQVEQGL
                     IAICEENLPDVKEYRGRGLVWGMEFHDKARAGRIARRAFELGLLVETSGPESEVVKLLP
                     ALTITPDELDEGLKILARAVRETA"
     aSDomain        3792..4805
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE308_RS33795_Aminotran_3.1"
                     /evalue="1.50E-74"
                     /label="IE308_RS33795_Aminotran_3.1"
                     /locus_tag="IE308_RS33795"
                     /protein_end="366"
                     /protein_start="28"
                     /score="243.0"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLM
                     RDGVTHGLDMSTTAKRAFLESFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGKVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALKELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALETYWTDGSAMEKQTRTRGEQVEQGLIAICEENLPDVKEYRGRGLVWGMEFH"
     gene            5001..5405
                     /locus_tag="IE308_RS33800"
                     /old_locus_tag="GCM10018783_68400"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="IE308_RS33800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68400"
                     /product="ectoine synthase"
                     /protein_id="WP_110627285.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.1e-54, bitscore:
                     178.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAASGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVVCTKGEAELTDHEAGKTYTITPGTMYLLDGHERHTLRVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5411..6298
                     /gene="thpD"
                     /locus_tag="IE308_RS33805"
                     /old_locus_tag="GCM10018783_68410"
     CDS             5411..6298
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436210.1"
                     /locus_tag="IE308_RS33805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68410"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_190220810.1"
                     /sec_met_domain="PhyH (E-value: 9.5e-40, bitscore: 131.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVTDLYPTRGTSEVTVPRQDPVVWGSPDTPGPIATADLQAYER
                     DGFLAVDQLITEDEVAVYKAELDRLVNDPAIRADERSIVEPKSKEIRSVFEVHRISEVF
                     ANLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSIALTENFATNGGLMIMPGSHRTFLGCAGATPEDNYKKSLQMQDAGTPSDEALTEL
                     ASEHGIRLFTGKAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENTAVEPFAAPVR
                     RPEFIGARDFTPVR"
     gene            complement(6395..7447)
                     /locus_tag="IE308_RS33810"
                     /old_locus_tag="GCM10018783_68420"
     CDS             complement(6395..7447)
                     /NRPS_PKS="Domain: Aminotran_5 (48-344). E-value: 5.3e-27.
                     Score: 86.6. Matches aSDomain:
                     nrpspksdomains_IE308_RS33810_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989745.1"
                     /locus_tag="IE308_RS33810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68420"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_190220811.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.2e-22, bitscore:
                     74.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFAPENTYLNTASNGLLPARTVAAVHRAVRMRAEG
                     RPLTPLYGDVEAARASFARIAGVPVERVATGASVAEYGALIAASLPAGAEVLTAEADFA
                     SLVNPFHMRGDLKVRAVPLDRIADAVRPDTALVAVSTAQSADGRVADLAAVRDAARAHG
                     ARIYLDASQSAGWLPIDPGTYDFLASVGFKWLLGPHGAAFFVGPEDPGSLAPLLAGWVA
                     GEKPWDSCYGPVTELARSARRFDISPSLFSWAGLRASLELIEELGVEAVHAHDTALADR
                     FRTGLAALGHEPVAAPGSAIVSVPGLGHRQPELSAAGIEVSDRAGNLRASFHLYNTPQD
                     VDRLLDVLSG"
     aSDomain        complement(6416..7303)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE308_RS33810_Aminotran_5.1"
                     /evalue="5.30E-27"
                     /label="IE308_RS33810_Aminotran_5.1"
                     /locus_tag="IE308_RS33810"
                     /protein_end="344"
                     /protein_start="48"
                     /score="86.6"
                     /tool="antismash"
                     /translation="TPLYGDVEAARASFARIAGVPVERVATGASVAEYGALIAASLPAG
                     AEVLTAEADFASLVNPFHMRGDLKVRAVPLDRIADAVRPDTALVAVSTAQSADGRVADL
                     AAVRDAARAHGARIYLDASQSAGWLPIDPGTYDFLASVGFKWLLGPHGAAFFVGPEDPG
                     SLAPLLAGWVAGEKPWDSCYGPVTELARSARRFDISPSLFSWAGLRASLELIEELGVEA
                     VHAHDTALADRFRTGLAALGHEPVAAPGSAIVSVPGLGHRQPELSAAGIEVSDRAGNLR
                     ASFHLYNTPQDVDRL"
     gene            complement(7536..8258)
                     /locus_tag="IE308_RS33815"
                     /old_locus_tag="GCM10018783_68430"
     CDS             complement(7536..8258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661357.1"
                     /locus_tag="IE308_RS33815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68430"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_190220982.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDDVEVVHRSFELDP
                     GRAKGDAGPVIPMLAKKYGMSEAQAQAAEDNVGAQAAAEGLEYRTRGRDHGNTFDMHRL
                     LHFAKEQGRQDQLVQIFYRANFAEERSVFTEGDERLVELAVEAGLDADAARKVLADPDA
                     YAAEVRADEREAAQLGASGVPFFVLDRKYGVSGAQPAEVFAQALAQAWGERSPLTLIDQ
                     GRGDADACGPDGCAVPQH"
     gene            complement(8346..8846)
                     /locus_tag="IE308_RS33820"
                     /old_locus_tag="GCM10018783_68440"
     CDS             complement(8346..8846)
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130468.1"
                     /locus_tag="IE308_RS33820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68440"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_190220812.1"
                     /transl_table=11
                     /translation="MISRRVIRTVLPAEAGAVAALHTRARSTYYPDGLPDDGTDWLAAW
                     RTAVERPDGQVLGVVEDGVLTGIASFRVPEGGPAHTVKLYQFHVEPGRWRTGVGTALHA
                     ACVEQWRADRRRAAVLDVHVDNARARAFYARQGWTADPDRPYAPGDHHLGLRLSLAVPR
                     PGE"
     gene            complement(8894..9502)
                     /locus_tag="IE308_RS33825"
                     /old_locus_tag="GCM10018783_68450"
     CDS             complement(8894..9502)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="IE308_RS33825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_68450"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_110627289.1"
                     /transl_table=11
                     /translation="MDAELPELPFRLRTYGPDGNWSYEGGVLTGWAGARQDRFVPPTGE
                     AVEPAADAPRLLGAPEGDFQLIARVTVGFGAAFDAGALYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFTVTGSSVWLRVSRTGRAFAFHASRDGERWTFVRLFTLGEEKE
                     TGAALVGFMTQSPLGEGCAVTFDHLEFRPAWPDDLRDGS"
ORIGIN
        1 atggagacca tcgcgcgggc cggggagaag cccagcgaac gggccgcctc catctgcccc
       61 ttgggcacgg cctggatgcc cgcgcggatc gtctccgcca tgtacgcggc ggccaccagg
      121 ccgagcgcga gcgccacctt gccgtaggtg ccgccggcga tctccgtgcc ggggaaggcg
      181 agcggcaccg ccaccccgat gaagacgaag atcagcaggg cgggcaggcc gcggaagatc
      241 tcgatgtaga gaccggcgac ccaccggtag ggccggacgg acgacagccg catcagcgcg
      301 atgaccatgc cgagcgccag cccgacggcg aagccggaca ccgtgtagag cacggtgttc
      361 ttcagcgcca tggtgatgac gtcggggaac atctgccgcg cgatgtcctt ctgcgcgaac
      421 tggttctgca gccggtccca gtcggccgtg accgcgaggg cgaccacggc ggcggcgaag
      481 acggcgtact gcacaccgcg tgacaggccg cgcttctggc gccgggtcag ccccttcttg
      541 cggggcggca cccgcaggtc tgtgtctgtg tcggtcatgg agttcaggac gcggaggcgg
      601 acggcgaggc ggcggacgcg tcgtacgggc cgatccactt ctcgtacagc ttcttgtacg
      661 tgccgtcggc cttggcgtcg gcgatcgcct tgttgatggc ctcgcgcagc ttggtgttgc
      721 ccttcttcac cgtgaagccg tactgctcgc cggtgttgag gttgtcgacg accttgaagg
      781 cgtcggcgtt ggccttgtcc ttcagccagc cctggacgac cgggtagtcg atgatgacgg
      841 ccttgacctg gccggtgcgc aggccgttga gcacggcgtc ggaggactcg aaggagaccg
      901 ggtcgaagcc ctgcttcttg gcgtagtcct cgccggtggt ctgcgcctgg gcgcccagct
      961 tcacgccctt ggacttcacg tcggcgagcg aggagacacc gctcttcttg tcgacgagga
     1021 cggcctgggt ggcgtcgaag tacgggtcgg agaagtcgac gttcttcttg cgctcctcgg
     1081 tgatcgtcat gccggcggcg gcgaggtcgc actggccgga gttgaggaac gcgccggtct
     1141 tgaagttctc gaacggcgtg tccacgatct tctgcttcac gccgaggttc ttggcgacga
     1201 ggtcgatgag ggcgacgtcg aagccctgca ccttgccgtc gatctccgac tggaacggcg
     1261 ggtacggcag gtgggtgcac gtggtcagct gacccgcctt gaccagctcg acaccgccgg
     1321 cggcggtctt cgagccgctg ccgccgtcgt cggtggaggt gcaggcggcc acgagcgcca
     1381 gcgcggccgc ggcggtgccg gcggccagga ggcggcgggt ccggcgcccg gggtgcgtgt
     1441 tcacggggag cctctcggta agggaacagg gagttccgat tataaggaga agtttgggtc
     1501 cctcaaatca aacccatggc tatcgggccg tacgacctaa gaagtcggcg gccagggtcc
     1561 tgtggacgag cggcgaacgg gcggccggac ggtgccggtt accctcgatc ttgccgggcc
     1621 ccgcggggtc cgcccccctg agttcggccc cttgagtgaa ggaagcaccg ccgtgacgca
     1681 cccgttcctg gacctggccc cgctcagcgc ggcgcgcttc gcgtcgatcg aggaccgtgt
     1741 ggcgcggctc ctgcacaccg ggcaggacgt gctgatcatg cagggcgagg cgctgctgcc
     1801 gctggagggg gcgatccgcg cggcggccgg tccgggcacg accgcgctga acgtgatcac
     1861 cgggccgtac gggcagacgt tcgggaactg gctgcgcgac tgcggggccg aggtgatcga
     1921 cctggcggtg ccgttccaca cggcggtcac cgcggagcag atccgggcgg ccctcgccga
     1981 gcgcccggag atcgacttcg tgtccctggt gcacgcggag gcggccaccg gcaacaccaa
     2041 cccggtcgcg gagatcgggc cggtggtgcg cgagcacggt gcactgttct acctggacgc
     2101 ggtggcgtcg gtcggcgcgg agccggtgct ggtggacgcg tggggcgtgg acctgtgcgt
     2161 gatcggcgcg cagaaggcga tgggcggacc ggcgggtgtg tcggcggtgt cggtcagcga
     2221 gcgggcctgg gcccggatgg cggcgaaccc gcgggcgccg cgccgctcct acctgtccct
     2281 gctggactgg aaggagcgct ggatcgacgc cggccgtacg gcgctgccgc acgctccggc
     2341 ccagctggag atgctcgcgc tggaggcgtg cctggagcgg atcgaggcgc agggcctgga
     2401 cgcggtgatg gcccggcacg cgcgcgcggc ggccgcgacc cgggcgggcg cgctcgcgct
     2461 gggcggcggc ctggagccgt acgtccatga cgcggcggac gccgcgccgg tcgccacgac
     2521 gctgcgggcg ccggccggga ccgacgcggt ggagctggtg gcgcgggcgc tggagagcga
     2581 cccggcgctg ccggtgagcg cgggcggcgg cgcgctggcc aaggagatga tccgggtcaa
     2641 ccactacggg cccgacgcca ccgcggcgac ggtccgcggt gccctcgtcg cgctgggcca
     2701 ggcgctcgcg gagcggggcc tgagcgtcga cgtggccgcc gcgctcggtg cggcggacgc
     2761 ggccgggcgg tagcggcggg cggccgggcg gcagctgccg ggcgccctcg aagggcgccc
     2821 tcccggaatt cctttcccgc attccccgtg tattccgcat tcctcgcgca tttccgaacg
     2881 atgaggttcc cgtcctcttc tgcccgcttc tcgcgaccct aaacgcaaag atttcgcgaa
     2941 cgcttcgcga ggtaattcag ggagattccg tgaggaccgg acggctgtga cacgttccac
     3001 atgacgcggc gatttgcgaa gttttccccg tgcaaatcgg tacaacgttc accaagctcg
     3061 cgcacctgtc cgcgcccgcg cgataacaca acggtcggcc gcagcaaagc ccatccggct
     3121 atgcattttt gaattgctct cggtaaattc aatccgcatg actgccgcac cagcagacct
     3181 gctcatcgac cgtccgtcgg tggccgacgg atccgcgctc tggcgtatcg cccgggactc
     3241 cgaggccctc gacctgaact cctcctacag ctatctgctg tggtgccgtg acttcgccgg
     3301 cacctccgcc gtggcgcgcc gggaggacgg gcagccgatc gggttcgtca ccgggtacgt
     3361 acggccggag cggccgcaca ccctgctcgt ctggcaggtg gccgtcgact cggcgtaccg
     3421 cgggcggggg ctggccgccg ccctgctcga cgggctgacc gcccggctcg ccgccgagcg
     3481 cgcgatcacc gaggtcgaga ccaccatcac ccccggcaac accgcctccg agcggctgtt
     3541 cacctccttc gcggcacgcc acggcgccac cgtcgagcgg gaggtcctgt tcgacgcggg
     3601 gctcttcccg gacggcccgc acgaccccga ggtcctctac cgcatcgggc ccctctccct
     3661 ctgatccggc accccgccca cacccccaca ccgaggagcg attcgtcgtg accatcaccc
     3721 agcccgacct gagcgtcttc gagaccctgg agtccgaggt gcgcagctac tgccgcggct
     3781 ggcccaccgt tttcgaccgt gcgcagggca gccggatgta cgacgaggac gggcacgagt
     3841 acctggactt cttcgccggc gccggttccc tgaactacgg gcacaacaac ccggtcctga
     3901 aacgggcgct gatcgactac ctgatgcggg acggcgtcac ccacgggctc gacatgtcga
     3961 cgacggccaa gcgggccttc ctggagtcct tccagaacct ggtgctgcgc ccgcgcgacc
     4021 tgccgtacaa ggtcatgttc ccgggtccga ccggcaccaa cgccgtggag tccgcgctga
     4081 agctggcgcg gaaggtgaag ggacgcgagg cgatcgtgtc gttcaccaac gccttccacg
     4141 gcatgtcgct gggctccctc gccgtcaccg gcaacgcctt caagcgggcc ggggccggta
     4201 tcccgctggt gcacggcacg ccgatgccgt tcgacaacta cttcgacggc aaggtcccgg
     4261 acttcctgtg gttcgagcgg ctgctggagg accagggctc cggcctgaac aagcccgccg
     4321 cggtgatcgt ggagaccgtg cagggcgagg gcggcatcaa cgtggcccgg cccgagtggc
     4381 tgcgcgcgct caaggagctg tgcgagcggc aggacatgct gctgatcgtc gacgacatcc
     4441 agatgggctg cgggcgcacc ggtgccttct tctccttcga ggaggcgggc atcaccccgg
     4501 acatcgtcac tgtgtccaag tccatcagcg gctacggcct gccgatgtcg ctgtgcctgt
     4561 tcaagcccga gctggacgtg tgggagccgg gcgagcacaa cggcaccttc cgcggcaaca
     4621 acccggcgtt cgtcacggcg acggccgcgc tggagacgta ctggaccgac ggctccgcca
     4681 tggagaagca gacccggacc cgcggcgagc aggtcgagca ggggctcatc gcgatctgcg
     4741 aggagaacct gcccgacgtc aaggagtacc ggggccgcgg cctggtgtgg ggcatggagt
     4801 tccacgacaa ggcccgcgcc gggcgcatcg cgcggcgcgc cttcgagctg gggctgctcg
     4861 tggagacctc cggcccggag agcgaggtcg tcaagctgct gccggccctg accatcaccc
     4921 cggacgagct ggacgagggc ctgaagatcc tcgcccgcgc cgtccgtgag accgcctgat
     4981 cgactaggag tggcaccacc gtgatcgtcc gatcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcatgtgaa ggccgcgtcc ggcacctggg agagcaagcg gatcgtcctc gccaaggagc
     5101 gggtcggctt ctccctgcac gagaccatcc tctacgccgg gaccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtcgtctgca cgaagggcga ggccgaactc accgaccacg
     5221 aggccgggaa gacgtacacg atcacccccg gcaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct gcgcgtcaag gaggacttcc actgcatctg cgtgttcaac ccgcccgtca
     5341 ccggacggga ggaccacgac gagaacggcg tctacccgct gctcaccgag cccgaggagg
     5401 tgtgacaccc atgaccgctg tcaccgacct gtaccccacc cgcggcacca gtgaggtgac
     5461 cgtgccccgc caggacccgg tcgtctgggg ctccccggac accccgggcc ccatcgccac
     5521 cgccgacctg caggcctacg agcgggacgg cttcctcgcc gtcgaccagc tgatcaccga
     5581 ggacgaggtc gccgtctaca aggcggagct ggaccggctg gtgaacgacc ccgccatccg
     5641 cgccgacgag cgctcgatcg tcgagccgaa gtccaaggag atccgctcgg tcttcgaggt
     5701 gcaccggatc agcgaggtgt tcgccaacct ggtgcgcgac gagcgggtcg tcggccgggc
     5761 ccggcagatc ctcggctcgg acgtgtacgt ccaccagtcc cggatcaacg tcaagccggg
     5821 cttcggcgcg agcggcttct actggcactc cgacttcgag acctggcacg ccgaggacgg
     5881 cctgccgaac atgcggaccg tgtccgtctc gatcgcgctg acggagaact tcgccaccaa
     5941 cggcggcctg atgatcatgc ccggctcgca ccggaccttc ctgggctgcg cgggcgccac
     6001 gccggaggac aactacaaga agtccctcca gatgcaggac gcgggcacgc cgtccgacga
     6061 ggcgctgacc gagctggcga gcgagcacgg catccggctg ttcaccggca aggccggctc
     6121 ggcgacctgg ttcgactgca actgcatgca cggctccggc gacaacatca cgccgtaccc
     6181 gcgcagcaac gtcttcatcg tgttcaacag cgtggagaac acggcggtgg agccgttcgc
     6241 ggcaccggtg cgccgaccgg agttcatcgg cgcgcgggac ttcacgccgg tgcggtaggc
     6301 gcttcctcag gacaccgggc cgggccccct cgtgtgggac ggggggcccg gcccggtcct
     6361 gtgccgtcag ccgtcagccg tcagaggccg atcgtcagcc ggagagcacg tcgagcaggc
     6421 ggtccacgtc ctgcggggtg ttgtagaggt ggaaggacgc ccgcaggttg cccgcgcggt
     6481 cggagacctc gatgcccgcg gcgctcagct cgggctggcg gtggccgagt ccggggacgg
     6541 agacgatggc ggagccgggg gcggcgacgg gttcgtggcc gagcgcggcg agacccgtgc
     6601 ggaaccggtc ggccagcgcg gtgtcgtggg cgtggacggc ttccacgccc agttcctcga
     6661 tcagttccag ggaggcgcgc agcccggccc agctgaacag gctcgggctg atgtcgaacc
     6721 tgcgtgccga gcgggccagt tcggtgaccg ggccatagca gctgtcccag ggcttctccc
     6781 ccgcgaccca gccggcgagg agcggggcga gcgagccggg gtcctcgggg ccgacgaaga
     6841 aggcggcgcc gtgcgggccg aggagccact tgaagcccac ggaggcgagg aagtcgtagg
     6901 tgccggggtc gatcggcagc cagccggccg actgggaggc gtcgaggtag atccgcgctc
     6961 cgtgggcccg ggccgcgtcc cggacggcgg cgaggtcggc gacgcggccg tcggcggact
     7021 gggcggtgct gaccgcgacc agcgcggtgt cggggcggac ggcgtcggcg atccggtcca
     7081 gcgggacggc gcgcaccttg aggtcgccgc gcatgtggaa cgggttcacc agggaggcga
     7141 agtcggcctc ggccgtcagg acttcggcgc cggcgggcag cgaggcggcg atcagggcgc
     7201 cgtactccgc gaccgaagcg ccggtcgcca cccgttcgac gggcaccccg gcgatccggg
     7261 cgaaggaggc gcgggcggcc tccacgtccc cgtacagcgg ggtgagcggc cggccctcgg
     7321 cccgcatccg caccgcgcgg tgcacggcgg cgacggtgcg ggccggcagc aggccgttgc
     7381 tcgcggtgtt caggtaggtg ttctccggtg cgaactcggc gcggacgagg ctctcgaagg
     7441 tctccatgga accactgtga gccctgacct tccccaagtc cattgcattc ttttacgtgg
     7501 tcacgcaaag gactgcttat ggatgggcca ccggatcagt gctgcggcac cgcgcacccg
     7561 tccggcccgc aggcgtccgc gtcgccccgg ccctggtcga tcagggtcag cggggagcgc
     7621 tcgccccagg cctgcgcgag ggcctgcgcg aagacctcgg cgggctgggc gccggagacg
     7681 ccgtacttcc ggtcgaggac gaagaacggc acaccgctcg cgccgagctg ggcggcctcg
     7741 cgttcgtcgg cgcgcacctc ggcggcgtag gcgtccgggt cggcgagcac cttgcgggcg
     7801 gcgtcggcgt ccagtccggc ctcgacggcc agctccacga gccgctcgtc gccctcggtg
     7861 aagacggagc gctcctcggc gaagttggcg cggtagaaga tctggacgag ctggtcctgg
     7921 cggccctgct ccttggcgaa gtgcagcagg cggtgcatgt cgaaggtgtt gccgtggtcg
     7981 cggccccggg tgcggtactc caggccctcg gcggcggcct gcgcgccgac gttgtcctcg
     8041 gcggcctggg cctgcgcctc gctcatgccg tacttcttcg cgagcatcgg gatgacgggc
     8101 ccggcgtcgc ccttggcgcg ccccgggtcc agttcgaagg agcggtgcac cacctcgacg
     8161 tcgtcgcggt gcgggaaggc cgcgagcgcc ttctcgaagc gggccttgcc cacgtagcac
     8221 caggggcagg cgatgtcgct ccagatctcg acgcgcaggg tcacggttca ctcactccag
     8281 tcgcaggggg gcacggagac gtcctcggcc tccgcaggag tgtatgaacg ttcaaacagc
     8341 cggtttcatt cccccggccg gggcaccgcc agtgacagcc gcaggccgag gtgatggtcg
     8401 ccgggggcgt acggccggtc ggggtccgct gtccagccct gccgggcgta gaacgcgcgc
     8461 gcccgggcgt tgtccacgtg cacgtcgagg acggcggcgc gccgccggtc ggcgcgccac
     8521 tgctcgacgc aggcggcgtg cagggccgta ccgacgccgg tgcgccagcg gccgggctcg
     8581 acatggaact ggtacagctt gacggtgtgc gcggggccgc cctccggcac gcggaaggag
     8641 gcgatgccgg tgagcacccc gtcctccacc acgcccagca cctgcccgtc cgggcgctcg
     8701 acggcggtcc gccaggcggc gagccagtcg gtgccgtcgt ccgggagccc gtccgggtag
     8761 tacgtcgacc gggcccgggt gtgcagggcg gcgacggccc cggcctcggc gggcaggacg
     8821 gtgcggatca cacgtcggct gatcatgccc gtcaggacgc gtaccgcggc ggcggggttc
     8881 cccggccgcc cgctcagctg ccgtctctga ggtcgtccgg ccaggccggc cggaactcca
     8941 ggtggtcgaa ggtcaccgcg cagccctccc ccagtggcga ctgggtcatg aagccgacca
     9001 gggccgcgcc cgtctccttc tcctcgccga gcgtgaagag gcggacgaag gtccagcgct
     9061 cgccgtcgcg ggaggcgtgg aaggcgaagg cacggccggt gcggctgacc cgcagccaca
     9121 ccgaactgcc cgtcacggtg aaggagttgg cgtcgtcgga gtgcccccgg gtgaccaccg
     9181 tgcacacggt gggcacgtcc ggggagtact ccaggcacag tttggcccag gcccgttcgc
     9241 cgacgtggac gtagagcgcc ccggcgtcga acgccgcgcc gaaccccacg gtgacccggg
     9301 cgatcagctg gaagtcaccc tcgggcgcgc ccagcagccg cggtgcgtcg gcggccggct
     9361 cgaccgcctc cccggtgggc ggcacgaaac ggtcctggcg tgcgccggcc cacccggtga
     9421 gcaccccgcc ctcgtaggac cagttgccgt cggggccgta ggtgcgcagc cggaagggga
     9481 gttcgggaag ctctgcgtcc atgggcggct tctaccgctc caggcgtccg ttgaaccgcc
     9541 gcggcagccc cagcgggttg tcgtcgcgca gctccggcgg cagcagggcc tcgggcgcgt
     9601 tctggtaggc gaccgggcgc agccagcgct cgatggccgt gccgcccacg gaggtggagg
     9661 tggaggtcgt cgccgggtac gggccgccgt ggtgctgggc agcggccacg gcgacgcccg
     9721 tcggccagcc gttgacgagc acacgtccgg cgagcggggt cagctcggcg aggatctcgg
     9781 tggcccggcc ctcgccggcg gcctcggcct ccgacacctg gaccgtcgcc gtgaggttgc
     9841 cgggcagccg ggacaggacg gccttgacct cgtcctcgtc gtcgtagcgg gcgacgacgg
     9901 tgaccgggcc gaagcactcc tccagcagca ggtcgtgctc gtcctcggcg gccagccggc
     9961 tcgccgggac ggtgaggaag ccggcgctga ccgtgtgctc gccgcccgcg cccggcgtca
    10021 ccggcgcgtc cacgtcgggc agctgggcgc gctcggcgac cccggcgacg aagttgtccc
    10081 gcatgcggtg gtcgagcagg acgccggggt cggtgttgct cacggcgtcg gtgagggact
    10141 tgaccaggcc gtcgccggcg gcgccggacg gcaccagcac caggccgggc ttcacgcaga
    10201 actggccgac gcccagcgtc atggacccgg cgagcccggc gccgatggcc tcggcgcgct
    10261 cggcggcggc ggcctcggtg accacgaccg ggttcaggga gcccagctca ccgtggaagg
    10321 ggatcggcac ggggcgcgcg gcggccgcgt cgaacagcgc gcgcccgccg cggatcgagc
    10381 cggtgaagcc ggccgcggcg accag
//
