LOCUS       NZ_BNBR01000001        11344 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces griseosporeus strain JCM 4766 sequence01, whole genome
            shotgun sequence.
ACCESSION   NZ_BNBR01000001
VERSION     NZ_BNBR01000001.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces griseosporeus
  ORGANISM  Streptomyces griseosporeus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 2104813)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 112X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014656055.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 05:04:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,365
            CDSs (total)                      :: 7,282
            Genes (coding)                    :: 7,186
            CDSs (with protein)               :: 7,186
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 61 of 96
            Pseudo Genes (internal stop)      :: 11 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:46:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1776880
            Orig. end                         :: 1788224
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNBR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11344
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11344
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11344
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6344](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6344
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    218..230
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            1172..2026
                     /locus_tag="IE308_RS07840"
                     /old_locus_tag="GCM10018783_16000"
     CDS             1172..2026
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997021.1"
                     /locus_tag="IE308_RS07840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16000"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_190217139.1"
                     /transl_table=11
                     /translation="MIRPLRRVTAVCALAAAALTACGTAPSGPPAHRQAAHPAPSVAVS
                     RPPTLAPGPAGLTPVFEHGPRAGREKTVALTFDADMTADQGPRAARGERFDNPGLIAAL
                     RALKVPATVFMTGRWAEQYPVQARTIGRDPLFEIANHSYSHYAFTGDCYGLPTVSAERM
                     RADVERAYAAFRRAGVRDAMPYFRFPGGCYDRQALKALSGTGVTAVQWDVVSGDAFATD
                     ADAVARQVLDGVRPGSVVVLHCTRSAAPTTERVVRTVVPELRARGYRFVKVSELIGATG
                     ARR"
     gene            2054..2233
                     /locus_tag="IE308_RS07845"
                     /old_locus_tag="GCM10018783_16010"
     CDS             2054..2233
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503632.1"
                     /locus_tag="IE308_RS07845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16010"
                     /product="hypothetical protein"
                     /protein_id="WP_110632007.1"
                     /transl_table=11
                     /translation="MSEDYCTIGTAEKPPKADGPPYAECVLCREPTEYPESYKGITLCP
                     VCEWQEAQRTACSG"
     gene            complement(2237..2908)
                     /locus_tag="IE308_RS07850"
                     /old_locus_tag="GCM10018783_16020"
     CDS             complement(2237..2908)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="IE308_RS07850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16020"
                     /product="ABC transporter"
                     /protein_id="WP_110632004.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLLRSQRWLPPVILYAAFLAVGVQAGQPVLDSLGY
                     AAAGLLPVAAWLVRICVTGEPPAARAVVAAAAGPARAHLACLLVAAATATVLGTAAVVL
                     VSLVGDPVGTDHRTRVPLLPAAGAGLLAVLVCVLLGTAVGALTAWPLLRSTGRAVPALL
                     LAALLALVVTGSPAQAAVEGLVTGSQSGKVPLPLLPLAAAALLTAGATALACALTARRA
                     P"
     misc_feature    3106..3121
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    3207..3222
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            complement(3208..4077)
                     /locus_tag="IE308_RS07855"
                     /old_locus_tag="GCM10018783_16030"
     CDS             complement(3208..4077)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034060.1"
                     /locus_tag="IE308_RS07855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16030"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_190217140.1"
                     /transl_table=11
                     /translation="MHRELRLRDVGRRYGPRGRWVIRHVDLAVAPGTLTRVEGANGTGK
                     STLLRVLAGLDAPSEGRVSGRPRTAYVPERFPAALPFTAAGYLTHLGTVHGLSRPEAAR
                     AARAWLERFDADAHAGTPLARLSKGSSQKVAVAQALLAEPGLLVLDEAWTGLDTAARDE
                     LDRAVGERTAAGGAVVFVDHDPRRLAGAVDAVWTVADGTLVRRTPAEDPSEPGPCVTVV
                     VQGPPPPPDVLRMTATATETAPGTHRLTVPATHSDTVLRALLTADPPRHVVRVAPATPP
                     TDTPGTHR"
     gene            4185..4904
                     /locus_tag="IE308_RS07860"
                     /old_locus_tag="GCM10018783_16040"
     CDS             4185..4904
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997025.1"
                     /locus_tag="IE308_RS07860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16040"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_190217141.1"
                     /transl_table=11
                     /translation="MNSEATPVQDSPFRPERSDRDTAPQFVLPMVVRIERAAPPARTDA
                     LETAARAVLVMLADERSVGDGEWAQAVRDWQDARIRKVVRRARGAEWRRAEALPGITVT
                     GKAAEVRVFPPVPLDGWPKDLARLQVSGTDLDDPEPPAAPDPAGPVLWMSPEVEMSAGK
                     AMAQAGHGAQLAWWELSGEERAAWRDSGFALSVRTADPDRWRDLTTSGLPVVRDAGFTE
                     IAPGSCTVVADHPALRP"
     gene            5001..6344
                     /locus_tag="IE308_RS07865"
                     /old_locus_tag="GCM10018783_16050"
     CDS             5001..6344
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130505.1"
                     /locus_tag="IE308_RS07865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16050"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_190217142.1"
                     /sec_met_domain="DUF692 (E-value: 5.7e-99, bitscore: 327.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIADAVERMPGIDWVEVVAENVCPGHLPDSLRR
                     LRERGVTVVPHGVSLGLGGADRPDEGRLRALAERAEALGSPLVTEHIAFVRAGGPLTAS
                     AHLEAGHLLPVPRTRDALNVLCENVRIAQDALPVPLAVENIAALFSWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGYERDGVWHDSH
                     AHPVPRPVLDLLTDLAAQVSPPGVLLERDENFPAPAELERELGSIRDAVERGGAAAGPR
                     QAGGGPVTEPRQASPRQSGHESACGAVDEVPRQRLALAQTALLSALVAGTPAPEGFDRV
                     RLGVQSRALAAKRAGVVAKVAPELPEILGELYRPAFLAYAHTHPMTAGYRHDALAFAAH
                     LLRERRVHDPAAHRELREWWLDRSGPEPRSHRPAARLARATRRVLLRR"
     gene            6435..7271
                     /locus_tag="IE308_RS07870"
                     /old_locus_tag="GCM10018783_16060"
     CDS             6435..7271
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997027.1"
                     /locus_tag="IE308_RS07870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16060"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_190217143.1"
                     /transl_table=11
                     /translation="MRPRPPIPGRRFFSGTGLIITGLAATLAALVFPIWSYADRSGTAA
                     ATLDAESVSTPYGPLSALDRDFITKVRLAGLWELPAGGLALRKGTTDAVRVAGRHLIEG
                     HTFLDQRVRDVGARLGLALPEEPNEQQRGWLKDLTAAQGTEFDRTFANVLRVAHGRVFS
                     VVAQVRAGTRNTLVRDLADDANTTVLDHIKVLEATGYVDYDAMARDLAADSAPPPAGSS
                     VPPGPVSDPPPALPATPGPTDPASPANPPDPADPATGYPLPPPAVSPPPSRAGTTG"
     gene            7370..8170
                     /locus_tag="IE308_RS07875"
                     /old_locus_tag="GCM10018783_16070"
     CDS             7370..8170
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981760.1"
                     /locus_tag="IE308_RS07875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16070"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_190217144.1"
                     /transl_table=11
                     /translation="MFWVLLLLPAWAVAGLACTRLCLAAVRAAAVERQDTAVVREHDLT
                     LYEAAFLSGGPARVADLTLVSMARQRRLLLAHTGWATVVDPRARDDIERSVIGAIGPEG
                     QSRIAPVRAAASAADAVRHLADRLVSAGLAVPDGARTTVASGMRQVRLAAVAVLALGAT
                     ALLLPVPSDLPRTLMALWFTLPLALTLSCLAIARIEVHPYGRWASPAGQRLLAALARRA
                     GGTASGDDRSFLATVAVRGVRAIGEPELRAAFSHRDAAFGERPY"
     misc_feature    8257..8270
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.35"
                     /tool="antismash"
     gene            8305..9903
                     /locus_tag="IE308_RS07880"
                     /old_locus_tag="GCM10018783_16080"
     CDS             8305..9903
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (113-487). E-value:
                     3.7e-21. Score: 67.8. Matches aSDomain:
                     nrpspksdomains_IE308_RS07880_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824197.1"
                     /locus_tag="IE308_RS07880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16080"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_190217145.1"
                     /sec_met_domain="PF00561 (E-value: 5.4e-24, bitscore: 82.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALPSAAGSLLLTVLAAAPAGGAPDVPGTGATAAELRGTLVA
                     TARAAAEGIAFGPCADAQDMPGSMQCGTVEVPLDYAHPDGRKINLTVSRVQATQRDPLN
                     SKRRVPRQGALVFNPGGPGGDGLYFPLTGLLPQWKRLGAAYDLIGYSPRGVGRSAPLSC
                     QDPRQLFKGPSQAPTHPTESYKRERIAQAKAYARGCARRAGSALRHYTSLNNARDLDVL
                     RAALGEHRLTFMGASYGTYFGALYATLFPSHVRRMVFDSAVNPDPEQIWYRNNLRQSAA
                     FESRWADFREWIARHDKVYGLGDTPEKVLRSYERARARLARKPAGGKVGPGQLQGAFLQ
                     AGYYDDYWPHRAHALSAYLKGDPRPLIEQAAPVTEAAAEAENADAVYTAIECNDAPWPT
                     DFRVWDRDNTRLARVAPFETWDNAWTNLPCAYWPAPRQQPLDVRTGPGELPPVLILAAE
                     RDAATPYDGALELHRRLSGSVLVTERDAGTHGLAGGPNRCVNGHLEAYLLEGRLPARRA
                     ACAGHAEPEPRAGGR"
     aSDomain        8644..9765
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE308_RS07880_Abhydrolase_1.1"
                     /evalue="3.70E-21"
                     /label="IE308_RS07880_Abhydrolase_1.1"
                     /locus_tag="IE308_RS07880"
                     /protein_end="487"
                     /protein_start="113"
                     /score="67.8"
                     /tool="antismash"
                     /translation="ALVFNPGGPGGDGLYFPLTGLLPQWKRLGAAYDLIGYSPRGVGRS
                     APLSCQDPRQLFKGPSQAPTHPTESYKRERIAQAKAYARGCARRAGSALRHYTSLNNAR
                     DLDVLRAALGEHRLTFMGASYGTYFGALYATLFPSHVRRMVFDSAVNPDPEQIWYRNNL
                     RQSAAFESRWADFREWIARHDKVYGLGDTPEKVLRSYERARARLARKPAGGKVGPGQLQ
                     GAFLQAGYYDDYWPHRAHALSAYLKGDPRPLIEQAAPVTEAAAEAENADAVYTAIECND
                     APWPTDFRVWDRDNTRLARVAPFETWDNAWTNLPCAYWPAPRQQPLDVRTGPGELPPVL
                     ILAAERDAATPYDGALELHRRLSGSVLVTERDAG"
     gene            complement(10009..10734)
                     /gene="hemQ"
                     /locus_tag="IE308_RS07885"
                     /old_locus_tag="GCM10018783_16090"
     CDS             complement(10009..10734)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130502.1"
                     /locus_tag="IE308_RS07885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_16090"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_190217146.1"
                     /transl_table=11
                     /translation="MSDERIPNERIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDRSG
                     YAEEVQELFDQLAAKDVTIRGTYDVSGLRADADLMIWWHAETSDQLQEAYNLFRRTRLG
                     RALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYVSVYPFVRSYDWYLLPDEDRRRML
                     TDHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRGSEARMHVREE
                     VPFFTGRRKDVSDLIAGLA"
     misc_feature    complement(10729..10744)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.23"
                     /tool="antismash"
     misc_feature    10732..10747
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.22"
                     /tool="antismash"
     misc_feature    11182..11197
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.35"
                     /tool="antismash"
ORIGIN
        1 cgggaggcgg ggctgccggc gtcgttcgtg acggagaccg agctgccgtt cccggtggcg
       61 ggcgcggtgg aggtcaccgg gcaggcgcag ttccatccgc gccgctacct cctcgccctc
      121 gtcgacgagc tgatcgcccg gggcggccag gtgtacgaga acacgcgggt gaccggcctg
      181 cgggagggcg agccgtgccg cgtgacgacc gagggcggtg ccgtggtcac cgcgaaggac
      241 gtggtggtcg cgacccacta cccggtcttc gaccgggcga tgctgttcac ccggctctcc
      301 ccgcgccgtg agctggtcgt cgcgggcccg atcgacgcgg agcgggaccc gggcggcatg
      361 tacatcacgc ccgaggagaa cacccgctcg gtgcggaccg cgccgtacgg tcccgggcag
      421 cggttcctgg tggtcaccgg cgagcacttc acgcccggca ccggcgacac ccgcgcccgc
      481 ttcgccgagc tggccggctg ggccacccgg cacttcccgg acctgcggat cacgcacacc
      541 tgggccacgc aggacaacga ccccaccgac accgtgccgc tggtcggccc gctgcacccg
      601 ggcgcccggc acacgtacgt ggcgaccggc ttcggcggct ggggaatgag cggcggcatc
      661 atggcgggcc ggctgctgac ctcgctgatc cggggcgagc cgtgcgcgtg gagcggactg
      721 tacgacccgc ggcggatgcg cagccaggtc cgcgaggcgc cggccttcct gaagaaccag
      781 gccaaggtgg cccggcactt cgtcggcgac cggctgcggc cgggccggtc cgtggcggac
      841 ctcggccccg gccagggcgc ggtggtccgc gtcggcgcgg accgcctggc cgtctaccgc
      901 gacgaggacg gcgagacgca cgccctgtcc gcgcgctgca cccacatggg ctgtctggtc
      961 tccttcaact ccgccgaacg cgcctgggag tgcccctgcc acggctcccg cttcgacacc
     1021 gacggcaccg tcgtgcaggg cccggcggtg aagccgctgg agcggcggcg gctgcgcggc
     1081 ccggacgagg agcccccggc cgggtgaacc accgccggac gcggggcgcg gcgcccccgg
     1141 acgccccgga aggcattcgt aatgtcatac ggtgatcaga cctctccgcc gtgtcaccgc
     1201 cgtctgtgcc ctggccgccg ccgcgctcac cgcgtgcggc acggccccct ccggtccgcc
     1261 ggcccatcgg caggccgccc accccgcgcc gtcggtggcc gtctcccggc cgccgacgct
     1321 cgcccccggc ccggcgggtc ttacccccgt cttcgagcac gggccgcggg ccggccggga
     1381 gaagacggtg gcgctcacct tcgacgccga catgaccgcg gaccaggggc cgcgcgcggc
     1441 acggggcgag cgcttcgaca acccggggct gatcgcggcg ctgcgcgcgc tgaaggtgcc
     1501 ggcgaccgtg ttcatgaccg gacggtgggc ggagcagtac ccggtccagg cccgcaccat
     1561 cggccgggac ccgctgttcg agatcgccaa ccactcctac agccactacg ccttcaccgg
     1621 tgactgctac ggcctgccga ccgtctccgc ggagcggatg cgggccgacg tggagcgggc
     1681 ctacgccgcc ttccgccggg cgggggtgcg ggacgcgatg ccgtacttcc gcttcccggg
     1741 cggctgttac gaccggcagg cgctgaaggc gctcagcggc accggggtca ccgcggtgca
     1801 gtgggacgtg gtgagcgggg acgcgttcgc cacggacgcg gacgcggtgg cccggcaggt
     1861 cctggacggg gtgcggccgg gttcggtggt ggtcctgcac tgcacccgca gcgccgcccc
     1921 gaccaccgag cgggtggtcc gcacggtcgt gcccgagctg cgggcgcgcg gctaccgctt
     1981 cgtgaaggtc tccgagctga tcggcgccac cggcgcccgg cgctgaccgg cccgcgcgcc
     2041 ctacgctggg ggtatgagcg aggactactg cacgatcggc acggccgaga agcccccgaa
     2101 ggcggacggc ccgccgtacg cggagtgcgt gctgtgccgg gagccgacgg agtacccgga
     2161 gtcgtacaag gggatcacgc tctgcccggt ctgcgagtgg caggaggcgc agcgcaccgc
     2221 ctgctcgggg tgacgctcag ggtgcccggc gggcggtgag cgcgcaggcc agggcggtcg
     2281 ccccggccgt gagcagggcc gccgcggcga gcgggagcag gggcagcggc accttcccgg
     2341 actgcgagcc ggtgaccagc ccctcgaccg ccgcctgggc cggtgacccc gtcaccacca
     2401 gcgccagcag ggccgcgagc agcagcgcgg gcacggcacg gccggtcgag cgcagcagcg
     2461 gccacgcggt gagcgcgccg acggccgtgc ccagcaggac gcagaccagc acggcgagca
     2521 ggcccgcacc ggccgccggg agcagcggca cgcgcgtgcg gtggtcggtg ccgaccgggt
     2581 cgccgaccag cgagaccagc acgacggcgg ccgtgcccag cacggtggcc gtggcggccg
     2641 cgaccagcag gcaggccagg tgggcgcggg cgggaccggc cgccgcggcg acgaccgcgc
     2701 gggccgcggg cggctcgccc gtgacgcaga tccgcaccag ccaggcggcg acgggcagga
     2761 gtcccgcggc ggcgtacccg agcgagtcca gcaccggctg cccggcctgg acaccgacgg
     2821 cgaggaacgc ggcgtacagg atcaccggcg gcagccagcg ctgcgagcgc agcagcaggg
     2881 cggcctgata gcgcagcagg gcggtcacgg acggctcctg ggggtgtcgg cgggcgggga
     2941 ggcaggggcg acgcggacga cgtggcgggg cggacggccg gtgggcaggg ggcgcgggat
     3001 cgcggtcacc ggtggctttc gggggtgtcg gtgggcgggg tggcgggggc gacgcggacg
     3061 acgtgtcggg gcggacggcc ggtgggcagg gggcgcgcga tcgcggtcac cggtgggtcc
     3121 cgggggtgtc ggtgggcggg gaggcggggg cgacgcggac gacgtgtcgg ggcggacggc
     3181 cggtgggcag ggggcgcggg atcgcggtca ccggtgggtc ccgggggtgt cggtgggcgg
     3241 ggtggcgggg gcgacgcgga cgacgtgtcg gggcgggtcg gcggtgagca gggcgcgcag
     3301 cacggtgtcg gagtgggtcg cggggacggt cagccggtgg gtgccggggg cggtctcggt
     3361 ggccgtggcg gtcatacgga gcacgtcggg cggcgggggc ggtccctgga cgacgacggt
     3421 gacgcacggg cccggctcgg acggatcttc cgccggggta cggcggacca gggtgccgtc
     3481 ggccaccgtc cagaccgcgt cgaccgcgcc ggcgaggcgc cgcgggtcgt ggtcgacgaa
     3541 gacgaccgcc ccgccggcgg cggtccgctc ccccaccgcc cggtccagct cgtcgcgcgc
     3601 ggcggtgtcc agcccggtcc acgcctcgtc cagcaccagc agccccggct cggcgaggag
     3661 ggcctgcgcg accgcgacct tctggctgct gcccttggac agccgcgcca gcggggtgcc
     3721 ggcgtgggcg tccgcgtcga accgctccag ccaggcccgc gccgcccggg ccgcctcggg
     3781 ccgggacagg ccgtgcaccg tgccgaggtg ggtgaggtag ccggccgcgg tgaacggcag
     3841 cgcggccggg aagcgttcgg gcacataagc cgtgcgggga cggccgctga cccggccctc
     3901 gctgggcgcg tcgagtccgg cgaggacacg cagcagggtg gacttgccgg tcccgttggc
     3961 gccctcgacg cgggtgagcg tgccgggcgc gaccgccagg tcgacatgcc tgatcaccca
     4021 ccgtccgcgc ggcccgtacc ggcgtcccac gtcgcggagt cgaagctcac ggtgcatgcg
     4081 ggccatcctc tcgtgccgtg gggcacgccc gggggccgat cgccgggaac cgcacagccc
     4141 gtgcggctcg gtcggcgtcc gggcggcttg gcagactgaa cggcgtgaac agtgaagcga
     4201 cccccgtcca ggacagcccc ttccgccccg agcgcagcga ccgcgacacg gcaccgcagt
     4261 tcgtgctgcc gatggtcgta cggatcgagc gcgccgcccc gcccgcgcgg accgacgcgc
     4321 tggagaccgc ggcgcgtgcc gtgctggtga tgctggccga cgagcggtcg gtgggcgacg
     4381 gcgagtgggc gcaggccgtg cgggactggc aggacgcgcg gatccgcaag gtagtgcggc
     4441 gggcgcgggg tgcggagtgg cggcgggccg aggcgctgcc cgggatcacg gtgaccggca
     4501 aggcggccga ggtgcgggtc ttcccgccgg tgccgctgga cggctggccc aaggatctgg
     4561 cgcggctcca ggtctccggc accgatctgg acgacccgga gccgcccgcg gcgccggacc
     4621 ccgccgggcc ggtgctgtgg atgagccccg aggtggagat gtcggccggc aaggcgatgg
     4681 ctcaggccgg gcacggcgcc cagctcgcct ggtgggagct gtccggcgag gagcgcgccg
     4741 cctggcgcga ctccggcttc gccctctcgg tgcgcaccgc cgacccggac cgctggcgtg
     4801 acctcaccac cagcggcctg ccggtggtgc gggacgcggg cttcaccgag atcgccccgg
     4861 gcagctgcac ggtcgtggcg gaccacccgg cgctacggcc ctgagcaccc aacctcaaat
     4921 gttgctctga acctccccgg cccgcggttc ggcaccgccg ggcggggcca tacccccctc
     4981 accgagaagg agggggagac atgcggcgac tgggtacggg catcggatgg cggccggaga
     5041 tcgcggacgc cgtggagcgc atgccgggca tcgactgggt cgaggtcgtg gcggagaacg
     5101 tgtgccccgg ccacctcccc gactcgctgc gccggctgcg cgagcggggg gtgacggtcg
     5161 tcccgcacgg cgtctccctc ggcctcggcg gcgccgaccg ccccgacgag ggacggctgc
     5221 gggccctcgc cgagcgggcc gaggcactgg gctcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtccgcgc gggcgggccg ctgaccgctt ccgcgcacct ggaggccggc cacctgctgc
     5341 cggtgccgcg cacccgggac gccctcaacg tgctgtgcga gaacgtccgt atcgcccagg
     5401 acgcgctgcc cgtgccgctc gcggtggaga acatcgccgc gctgttctcc tggcccggcg
     5461 aggagatgac ggagggccag ttcctgtacg agctcgccga ccgcaccggc gtgcggctcc
     5521 tcatcgacgt ggccaacctg cacaccaacc acgtcaaccg cggcgaggac ccggccaagg
     5581 cgctcgccga actcccgctg gaggccatcg cctacgtcca tgtcgcgggc ggctacgaac
     5641 gcgacggcgt ctggcacgac agccacgccc accccgtccc gcgcccggtc ctggacctcc
     5701 tcaccgacct cgccgcgcag gtcagcccgc ccggggtcct cctggaacgg gacgagaact
     5761 tccccgcacc ggcggagctg gagcgtgagc tgggatccat ccgggacgcg gtggagcggg
     5821 gcggggcggc ggccgggccc cggcaggccg ggggcgggcc ggtgaccgag ccccggcagg
     5881 cctcgccacg tcagagcggg cacgagtcgg cgtgcggcgc ggtggacgag gtgccccggc
     5941 agcggctcgc cctcgcccag acggcgctgc tgtccgcgct ggtggcgggc acccccgcgc
     6001 cggagggctt cgaccgggtc cgcctcggtg tccagtcccg cgcgctggcc gccaagcggg
     6061 ccggggtggt ggccaaggtc gccccggagc tcccggagat cctcggcgag ctgtaccgcc
     6121 ccgccttcct cgcgtacgcc cacacccacc ccatgaccgc gggctaccgc cacgacgccc
     6181 tcgccttcgc cgcgcacctg ctgcgcgagc gccgggtgca cgacccggcg gcgcaccggg
     6241 agttgaggga gtggtggctg gaccgctcgg gccccgagcc gcgctcgcac cggccggcgg
     6301 cccggctggc gcgggccacc cggagggtgc tgctgcgcag gtgaggcggg ccggggccgc
     6361 ccggcacccg caccgcccga ccggacggcc gggcaccccg cagtaatatt cggtgccgca
     6421 agcaggaggg caccatgcga ccccgacccc ccatacccgg ccgacgattc ttcagcggca
     6481 ccggtctcat catcaccggc ctcgcggcca ccctcgccgc cctggtgttc ccgatctggt
     6541 cgtacgccga ccgctccggc accgcggccg ccaccctcga cgccgagtcc gtgtcgacgc
     6601 cgtacggacc gctgtccgcc ctggaccggg acttcatcac caaggtgcgc ctggcgggcc
     6661 tgtgggagct gcccgccggc gggctggcgc tgcggaaggg caccacggac gccgtacgcg
     6721 tcgcgggccg gcacctgatc gaggggcaca ccttcctgga ccagcgggtc cgcgacgtcg
     6781 gggcgcggct cgggctggcg ctgcccgagg agccgaacga gcagcagcgg gggtggctga
     6841 aggacctcac cgcggcgcag ggcacggagt tcgaccggac gttcgccaat gtgctgcggg
     6901 tcgcgcacgg ccgggtgttc tcggtcgtcg cccaggtccg cgcgggcacc cgcaacaccc
     6961 tcgtccgcga cctggccgac gacgccaaca ccacggtcct ggaccacatc aaggtgctgg
     7021 aggccaccgg gtacgtcgac tacgacgcga tggcccgcga cctcgccgcc gacagcgctc
     7081 cgccgcccgc cggctcctcg gtgccgcccg gcccggtgtc cgacccgccg ccggcgctcc
     7141 cggccacccc cggccccacg gacccggcga gcccggcgaa cccgcccgat ccggcggacc
     7201 cggcgaccgg ttacccgctg ccgccgcctg ccgtcagtcc gccgccgagc cgggccggga
     7261 cgaccggctg agcgggccaa ccgaacggaa cgtcacatac cggcaacgct gtgtccggat
     7321 aatgaacagg gcatggcgcg gccccttccc cctggcatag aaacacggca tgttctgggt
     7381 ccttctcctg ctgccggcct gggccgtcgc cggcctcgcg tgcacccggc tgtgcctggc
     7441 cgccgtccgc gcggcggcgg tcgagaggca ggacacggcc gtggtccggg agcacgatct
     7501 gacgctgtac gaggccgcgt tcctctccgg cggccccgcc cgggtcgccg acctgacact
     7561 ggtgtcgatg gcccggcagc gccggctgct gctcgcccac accggctggg ccaccgtcgt
     7621 cgacccgcgc gcccgggacg acatcgagcg gtcggtgatc ggggcgatcg gaccggaggg
     7681 ccagtcccgt atcgcgccgg tacgggccgc cgcgtccgcc gccgacgcgg tccgccacct
     7741 cgccgaccgg ctggtcagcg ccgggctcgc ggtccccgac ggggcccgta cgacggtcgc
     7801 ctccggcatg cgccaggtac ggctcgccgc ggtcgcggtc ctcgccctcg gcgccaccgc
     7861 gctgctgctg ccggtgccgt cggacctgcc gcgcaccctg atggccctct ggttcacgct
     7921 gccgctcgcg ctcaccctga gctgcctggc gatagcccgg atcgaagtgc acccgtacgg
     7981 gcgctgggcc tcccccgccg gtcagcgcct gctggccgcc cttgcccggc gcgcgggcgg
     8041 caccgcgagc ggcgacgacc gctccttcct cgccaccgtc gccgtccggg gcgtgcgggc
     8101 gataggcgag ccggagctgc gggccgcgtt ctcccaccgc gacgccgcct tcggggaacg
     8161 gccgtactga ccggcaccgc cggccgcggc cgcccggggg cgcacagggg gcattgacgc
     8221 cgacaccggc gaacggtgct tgccttcccc accccgcgaa ccaacgatcc cttgtgtcgc
     8281 cgccgcgcac gaaagggatc cgccatgcga gctgccgccc tcccctcggc cgccgggtcg
     8341 ttgctgctga ccgtcctggc cgccgccccg gccggcggcg cgccggacgt ccccggcacc
     8401 ggggccaccg ccgccgagct gcgcggcacg ctggtggcca ccgcgcgggc cgcggccgag
     8461 ggcatcgcgt tcggcccctg cgccgacgcc caggacatgc ccggcagcat gcagtgcggc
     8521 acggtcgagg tgccgctcga ctacgcccac ccggacggcc ggaagatcaa cctcaccgtc
     8581 agccgggtgc aggccaccca gcgcgacccg ctcaacagca agcgccgcgt cccccggcag
     8641 ggcgccctcg tcttcaaccc gggcggcccc ggcggcgacg gcctgtactt cccgctgacc
     8701 ggcctgctgc cgcagtggaa gcgcctcggc gcggcctacg acctgatcgg ctactccccg
     8761 cgcggcgtcg gccgctccgc ccccctgtcc tgccaggacc cccgacagct gttcaagggg
     8821 ccgtcgcagg cgccgacgca ccccacggag tcgtacaagc gggaacggat cgcgcaggcg
     8881 aaggcgtacg cgcgcggctg cgcccggcgg gcgggcagcg cgctgcggca ctacacctcc
     8941 ctgaacaacg cccgcgacct ggacgtcctg cgcgccgcgc tcggcgagca ccggctgacc
     9001 ttcatgggcg cctcctacgg cacctacttc ggcgcgctgt acgcgacgct gttcccctcc
     9061 cacgtacggc ggatggtgtt cgactcggcg gtcaacccgg acccggagca gatctggtac
     9121 cgcaacaacc tgcggcagtc cgccgcgttc gagagccgct gggcggactt ccgggagtgg
     9181 atcgcccggc acgacaaggt gtacggcctc ggcgacaccc ccgagaaggt actgcgcagc
     9241 tacgagcggg cgcgcgcccg gctggcgcgg aagcccgccg gcgggaaggt cggcccgggg
     9301 cagctgcagg gcgcgttcct ccaggccggc tactacgacg actactggcc gcaccgcgcg
     9361 cacgccctgt ccgcctacct gaagggcgac ccgcggccgc tgatcgagca ggccgcgccg
     9421 gtcaccgagg cggccgccga ggcggagaac gcggacgcgg tctacacggc gatcgagtgc
     9481 aacgacgcgc cctggccgac ggacttccgc gtctgggacc gggacaacac ccggctcgcg
     9541 cgcgtcgcgc cgttcgagac gtgggacaac gcctggacga acctgccctg cgcctactgg
     9601 cccgcgcccc ggcagcagcc cctcgacgta cggaccgggc cgggtgagct gccgccggtg
     9661 ctgatcctgg cggccgaacg ggacgcggcc acgccgtacg acggggcgct ggagctgcac
     9721 cggcggctgt ccggctcggt actggtcacc gagcgggacg ccggcacgca cggcctcgcc
     9781 ggcgggccca accggtgcgt caacgggcac ctggaggcgt acctgctgga ggggcggctg
     9841 ccggcgcggc gggcggcgtg cgcggggcac gccgagcccg agccgcgcgc gggcgggcgc
     9901 tgaccggcgc cctgaacagg cctgttcggg cgtgcacagg cctgttcggg cctgaccagg
     9961 cctgttcagg cctgaccagg tatgggaccg gtcaggtcgg gggcggcgtc aggcgaggcc
    10021 cgcgatcagg tccgagacgt ccttgcggcg gccggtgaag aacggcacct cctcgcggac
    10081 gtgcatccgg gcctccgagc cgcgcaggtg gcgcatgagg tcgacgatgc ggtacagctc
    10141 gtcggcctcg aaggcgagga tccactcgta gtcgccgagc gagaacgagg cgacggtgtt
    10201 ggcgcgcacg tccgggtagc cgcgggccat cttgccgtgg tcggtgagca tccggcggcg
    10261 gtcctcgtcg ggcagcagat accagtcgta ggagcgcacg aaggggtaga cgctcacgta
    10321 gttgcggggc gtctcgtcgg cgaggaacgc cgggatgtgc gagcggttga actcggcggg
    10381 gcggtgcagc gccatgttcg accagaccgg ctccagggcg cggcccagcc tcgtgcggcg
    10441 gaagaggttg tacgcctcct gcagctggtc gctggtctcc gcgtgccacc agatcatgag
    10501 gtcggcgtcg gcgcgcaggc cggacacgtc gtaggtgccg cggatcgtca cgtccttcgc
    10561 ggcgagctgg tcgaacagct cctggacttc ctcggcgtac cccgagcggt cctcgggcag
    10621 cacgtccttg agcttgaaga cggaccacag cgtgtagcgg atgacctcgt taaggtcctt
    10681 ggccagcttg cccttgttcg ggatgcgctc gttcgggatg cgctcgtcac tcatggttct
    10741 tattctcccg ctccgccgtg caggctctgc accgggtggg cggtgagctg ctgcacaccg
    10801 gtgagatcgc cgccgagctg gtccacggcg gcgtacgcgc tcgcgatgca cgcggggatg
    10861 ccgacgccgt cgtacggcgc cccgcacacc gcgaggcccg gcagtgcggc gacgtgcgca
    10921 cggatgcggg ccacgcgcgc gtggtggccg acggggtact ggggcaggcc gtcgtcccag
    10981 cgggtgacgc gggtgcggag cggggtggcg tccaggccgg tggcctcccg caggtcgcca
    11041 cgggagatct cgaccaggtc gtcgtcgtcc cgctggagga tctccgtctc gccgtaccgg
    11101 ccgagcgagg tgcgtacgac caccacgtcc gggtcctcct cggcgatcca ggcccacttg
    11161 tgcgaggcga atgtcgacgc cttgatcgtg tggtcgtcga ccggcgggac gaggaagcca
    11221 ctgccctcgg ggaggcgggc ttcggtgtcg gagcggcggt aggcgagggt gaccagggcc
    11281 atggaggcgt attcgatcgc gcgcagctcg gcggcggcgg gcggggactc ggggccgagg
    11341 aggc
//
