LOCUS       NZ_BNBR01000001        11929 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces griseosporeus strain JCM 4766 sequence01, whole genome
            shotgun sequence.
ACCESSION   NZ_BNBR01000001
VERSION     NZ_BNBR01000001.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces griseosporeus
  ORGANISM  Streptomyces griseosporeus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 2104813)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 112X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014656055.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 05:04:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,365
            CDSs (total)                      :: 7,282
            Genes (coding)                    :: 7,186
            CDSs (with protein)               :: 7,186
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 61 of 96
            Pseudo Genes (internal stop)      :: 11 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:46:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1526576
            Orig. end                         :: 1538505
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNBR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11929
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11929
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11929
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6929](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6929)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(319..819)
                     /locus_tag="IE308_RS06730"
                     /old_locus_tag="GCM10018783_13750"
     CDS             complement(319..819)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293364.1"
                     /locus_tag="IE308_RS06730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_13750"
                     /product="hypothetical protein"
                     /protein_id="WP_190216978.1"
                     /transl_table=11
                     /translation="MLRTVEITAELTGQLKGDDFHALHFGPRLEFAVDCFICERTGRTQ
                     VFERGAERALCSGTRSGLARHYTPGRIAAYDTTSGAERLALRALVDFWWAPFEDKKTGA
                     PCPAAPSHPWVRLHLGYHCPTAGEPVRTSLQTNVARPCDLRCTHCKAVLATDATTPAVR
                     VLA"
     gene            complement(853..2037)
                     /locus_tag="IE308_RS06735"
                     /old_locus_tag="GCM10018783_13760"
     CDS             complement(853..2037)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656819.1"
                     /locus_tag="IE308_RS06735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_13760"
                     /product="cytochrome P450"
                     /protein_id="WP_190216979.1"
                     /sec_met_domain="p450 (E-value: 2e-23, bitscore: 79.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEVIDLGRYGDAFRTDPHPVYAELRARGPVHRVRPPGAEPEHET
                     WLVVGYEEARAALADPRLAKDPAKLGMTFLDEELIGKYLLSVDPPDHTRLRSLVSRAFT
                     ARRVERLRPRVQQITDDLLDAMLPQGRADLVESFAFPLPLTVICELLGVPELDRAEFRK
                     TSTEVVAPSSSGSEYDAVVRLADYLTELIEDKRCSGPSDDLLSDLIRTTAEDGDRLSAG
                     ELRGMAYLLLLAGHETTVNLIANGVHALLTHPGQLAALRADMGLLDGAVEEMLRYEGPV
                     EAATFRFAAEPLEIAGVPIAQGETVLVGLTAADRDPARYADPGRFDIRRDPRGHVAFGH
                     GIHFCMGAPLARLEARTAIRALLERAPGLALDGPPGPWLPGLLIRGMRSLPVRW"
     gene            complement(2117..2800)
                     /locus_tag="IE308_RS06740"
                     /old_locus_tag="GCM10018783_13770"
     CDS             complement(2117..2800)
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431807.1"
                     /locus_tag="IE308_RS06740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_13770"
                     /product="response regulator transcription factor"
                     /protein_id="WP_190216980.1"
                     /transl_table=11
                     /translation="MTAAIRVLLVDDDPLVRAGLSLMMGGADDIRIVGEAGDGDQVEAL
                     VDRTRPDVVLMDIRMPAVDGLTATERLRARPDAPQVVVLTTFHADEQVLRALRAGAAGF
                     VLKDTPPAEIVAAVRAVAAGDPVLSPAVTRQLMAHAAGTAGDTRRTRARARLALLNDRE
                     REVAVAVGRGLSNAEIAATLFMSVATVKAHVSRVLAKLGLNNRVQIALLAYDAGLTEED
                     LPDGH"
     gene            complement(2901..4157)
                     /locus_tag="IE308_RS06745"
                     /old_locus_tag="GCM10018783_13780"
     CDS             complement(2901..4157)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020119056.1"
                     /locus_tag="IE308_RS06745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_13780"
                     /product="sensor histidine kinase"
                     /protein_id="WP_229908721.1"
                     /transl_table=11
                     /translation="MKDDGTVPAPAPVLAGRRWWLPSALAHALELDLDAGRGGRPRRTA
                     RDWVVDCSCFLLALLVGFTAAQPLHDNPHIPHALAVTDQTLGALACCAVWLRRRLPVAL
                     AVALTAVNFVSETAGGACLIALFTLAVHRPFRYVAWIGGAQLALTTLYYWWRPDPEMPY
                     LVSVTFVVVLTGAVIGWGMFVRSKRQLLLSLRDRARRAETEARLRAEQAQRLAREDIAR
                     EMHDVLAHRLTLLSVHAGALEFRPDAPREEIARAAGVIRESAHEALQDLREIIGVLRAG
                     DRDDTGRPQPTLAALDALVAESGAAGMKVTLDQRVTDPDAVPASAGRTVYRVVQEALTN
                     ARKHAPGAEVTVTVTGARGEGLSVTVHNPPPDRPAPPVPGSGQGLIGLTERATLAGGRL
                     DHGPGADGGFTVRAWLPWG"
     gene            complement(4279..4968)
                     /locus_tag="IE308_RS06750"
                     /old_locus_tag="GCM10018783_13790"
     CDS             complement(4279..4968)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569704.1"
                     /locus_tag="IE308_RS06750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_13790"
                     /product="DUF5825 family protein"
                     /protein_id="WP_308440080.1"
                     /transl_table=11
                     /translation="MMTQTAEKPAAARISVTAWRDLDDEARTLPGMALGTIDLPAETGG
                     TGETCDGLWRLGARRVALHEPVDLSADGPAAARRTVDLLCLVRELTARAVQVDWDLRLP
                     ADGGAEQWKLLSHLHPPRRIDGLPEADAALYAWRTHHYLCKFVWRQGPGFVQIRDRRWG
                     ELRRFTTDEPAYHAAIAALDAGTGHHEIPPPVLADLTGEHLVLRVGDLAWWLPYRVNRW
                     LQEAMAI"
     gene            complement(5001..6929)
                     /locus_tag="IE308_RS06755"
                     /old_locus_tag="GCM10018783_13800"
     CDS             complement(5001..6929)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129143.1"
                     /locus_tag="IE308_RS06755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_13800"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_190216981.1"
                     /sec_met_domain="TIGR03975 (E-value: 5.5e-189, bitscore:
                     626.1, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2e-11, bitscore: 41.4,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILKRSVDERVPDATCDVLHANLEF
                     TDWITRRTEFTAQDYEYYALSSYFLGCGDWVFSSALYDDPEWKDEEFTRVMTPKLRRSR
                     MRMTKELHRVVPEFVEEIARAIVAAEPDVVGFTSTFQQNTAALAAARHVKALAPHIVTV
                     MGGANCDSEQGAAVHRNFPFVDHVVRGEGESAFPALLTALRDGTGDLTAVPGLCHRAPD
                     GSSVANPMATSPLPPAAILPPDYSGYFERLAKSVARNWVEPKLVVEGARGCWWGEKHHC
                     TFCGLNGSFMQFRSKSPETFYDEIMDLAARHRVLDMYVVDNILDMKYLSTVLPRIIDSG
                     YDLRMHIEIKANMRRAQLKTLADAGLIYVQPGIESLNSRVLDLMDKGVSGCQNVRMLRD
                     AAETGLSVSWNYLHGFPGETDDDYAPVIRQIPALEHLNPPVDLSARIAIERFSPYFDKP
                     ELGFTGLRPEQHYRFTYDLPEEELYDLAYVFEAPARGIGEDTVEALNAALALWRKRHVD
                     ARLTHMDLGERIVLVSRRSGFAWRTRELTDPYEIAVFRLLDQPHSVAALRRKAAEETSE
                     PRGEHEVRALLDGWVDDGIVFTDAGQYVHLAPSAVNQDLLRLDFMRHTHRVRADGPAAR
                     AEEPALS"
     misc_feature    complement(7108..7122)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.76"
                     /tool="antismash"
     gene            complement(7151..8989)
                     /locus_tag="IE308_RS06760"
                     /old_locus_tag="GCM10018783_13820"
     CDS             complement(7151..8989)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569707.1"
                     /locus_tag="IE308_RS06760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_13820"
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="WP_190216982.1"
                     /sec_met_domain="Peptidase_S9 (E-value: 8.6e-35, bitscore:
                     116.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTAPRYQVFRSALPEACPRDPARMVFTADADGRCEVFSWEATS
                     RRARQVTSRPAGTFHCAIDHDAGIWWFDEDPDGHGTWRHRPFSGELAGGLPDSPGLPGL
                     PPGLPCGLAFAADGTTAVALSDGRTATVHLGRKGHPARRLLTAPAPLTLHGIAPDGGLL
                     AVTGADGAVTLLDPAGAVTAVSPAGAARQWCRGFRPDGGRELLLVHQTDDHYLLATWTP
                     AAGTTVHDWCAFDTEITARWYPDSRAVLVRQDRHGRSLLHRVDLTARRRTRVPTPPGTI
                     LDAAPRPGGGLHYLWTDAAHPPVLRATDDTPLPPVTGTPPHVPGHHQDLWTPTPDGPVH
                     TLLSLPGTAAPGRPAPLVFLVHGGPADQDRDAYDATVHSLVASGYSVARVNYRGSTGYG
                     PRWRRAFTAGVGHTQVQDLAAVRADLLRRGLAAPDATALWGVSWGAYLVLLALGTRPGL
                     WQAGIAVKPLADWAAAHRSSTSALRALDLRLFGGTPDEVPERYARSSPLSYAPRVTAPL
                     LVVGATRDTKCPPDQIRSYLSALDAAGVPHEAMWLDTGHDGYDGAAHVAVLRRAMTFLS
                     RRLTAPPGPVTPDSPGQRGSPEPPLTRRPGPARTTH"
     misc_feature    complement(8717..8719)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(8945..8947)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8986..10296)
                     /locus_tag="IE308_RS06765"
                     /old_locus_tag="GCM10018783_13830"
     CDS             complement(8986..10296)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.5"
                     /locus_tag="IE308_RS06765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_13830"
                     /product="MFS transporter"
                     /protein_id="WP_190216983.1"
                     /transl_table=11
                     /translation="MNEPSSPASPAPAPRKSRRDLRILWWTNAIDGLGTQASGIVLPLL
                     ILDLGHPAAAAGSFASAVAVAGVVLGPVVARPADHGHRRALLIGSAALAAAAMSGLTAA
                     LFWWRPPLWAVLGLAFTERLCAFAHEAAARGALAHVARPEDVTRGAAGLQAGDQAALVV
                     GPGLGGALFEAARPLPFLADALSYAATALGIRALRAPLDTPVAPGRRRTPVTSGFRTVL
                     GSAPLRMVLVWASAASVLVTLLSYTAMFVLDGGTTVGLVLSASGAAGLLGALLAPGLVI
                     RLGARRAVVLATWLLLVPAGVLVTADAAASYATGFALLALLLPTVTVVLSGVLVRAVPR
                     EVQSQAGAAVGAAAALAAAGCPALAAGLVTWRGPHTPAVLCLALLALLAVYTQGAAGRS
                     LTAEADARPRAEETDARPRTDQPAPAARPSAGEDERP"
     misc_feature    complement(10360..10375)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.54"
                     /tool="antismash"
     gene            complement(10824..11450)
                     /locus_tag="IE308_RS06770"
                     /old_locus_tag="GCM10018783_13840"
     CDS             complement(10824..11450)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984013.1"
                     /locus_tag="IE308_RS06770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018783_13840"
                     /product="hypothetical protein"
                     /protein_id="WP_190216984.1"
                     /transl_table=11
                     /translation="MPGRTAVPPPPPARRPPRVLSWRLRRNPLRRRSDLVQGWIGLALL
                     LTASAATPAAVVLAADAAHDRATQTARRQAQTRHATTATLLESARHHPEPGSAEARGTR
                     YPAQVRYTDPEGRPRTATTDVPPALSKGSTLRVWVGADGQLTEPPLTPDQIRNRVRGSA
                     IIAALGVPAASIAAYGVTSRVVQRRQLAAWDRAWADTAPRWTAAP"
ORIGIN
        1 caccgagccg accggtccgc ccgccgccga caagccctgg ccgggtttcc tggagcgctt
       61 cgcgcccgcc tacgaggccg agctggcggc gttcgtggag gtgatccgcg gcgagcggcc
      121 caacccgtgc gacggccggg aggcgctgca ggccctgcgc atcgccgagg cgtgcgagct
      181 gtcacgacgg gagcgcaggc cggtacggct ggcggagatc ccgggcggcg cgggtacggc
      241 ggcgggctga ggacgtcccc ttcccctccc ccggcacgcg tcccggagca cccgctctcc
      301 cctctgcgcc gcgcgccgtc aggccagcac ccgcaccgcc ggtgtcgtcg cgtccgtcgc
      361 cagcaccgcc ttgcagtggg tgcagcgcag gtcgcagggc cgggccacgt tggtctggag
      421 gcttgtccgg accggttcgc cggccgtggg gcagtggtag ccgaggtgca gccggaccca
      481 ggggtggctg ggggcggccg ggcagggggc gccggtcttc ttgtcctcga agggcgccca
      541 ccagaagtcg acgagggcac gcagggcgag ccgctccgcg cccgaggtgg tgtcgtacgc
      601 ggcgatccgg ccgggcgtgt agtgacgggc gaggccgctg cgggtgccgg agcacaacgc
      661 ccgctcggcc ccgcgctcga agacctgggt gcgcccggtg cgctcgcaga tgaagcagtc
      721 cacggcgaac tccagccgcg gaccgaagtg cagggcgtgg aagtcgtcgc ccttgagctg
      781 tcccgtcagc tcggcggtga tctcgacggt gcgcagcacg cgggcccccc tcggtgtgtc
      841 ggacgtggct ttctaccagc gcacgggaag gctgcgcatc ccgcggatca gcaggccggg
      901 cagccagggc ccgggcggcc cgtcgagggc gaggcccggg gcccgctcca gcagggcgcg
      961 gatcgccgtg cgggcctcca ggcgggccag cggggcgccc atgcagaagt ggatgccgtg
     1021 gccgaaggcg acatggccgc gcgggtcgcg gcggatgtcg aagcggcccg ggtcggcata
     1081 gcgggcgggg tcgcggtcgg cggccgtgag gccgaccagc accgtctcgc cctgggcgat
     1141 gggcaccccg gcgatttcca gcggctcggc ggcgaaccgg aacgtggcgg cctccaccgg
     1201 gccctcgtag cgcagcatct cctcgacggc tccgtcgagc aggcccatgt cggcgcgcag
     1261 ggcggcgagt tggccggggt gggtgagcag ggcgtgcacg ccgttggcga tgaggttgac
     1321 ggtcgtctcg tggccagcga ggagcagcag gtacgccatg ccgcgcagtt caccggcgga
     1381 gagccggtcg ccgtcctcgg cggtggtgcg gatcaggtcg ctgagcaggt cgtcgctcgg
     1441 accgctgcag cgcttgtcct cgatcagctc ggtgaggtag tcggcgagcc gcaccaccgc
     1501 gtcgtactcg ctgcccgagc tgctgggcgc gaccacctcc gtcgacgtct tgcggaactc
     1561 ggcgcggtcc agctcgggaa cgccgagcag ttcgcagatg acggtgaggg gcagcgggaa
     1621 ggcgaacgac tccacgaggt cggcgcggcc ctgcggcagc atcgcgtcga gcaggtcgtc
     1681 ggtgatctgc tggacgcgcg ggcgcagccg ctccacgcga cgcgcggtga aggcgcggga
     1741 gacgagcgag cgcagccggg tgtggtccgg cgggtcgacg ctgagcaggt acttgccgat
     1801 cagctcctcg tcgaggaagg tcatgccgag cttcgcgggg tccttggcga gccgggggtc
     1861 ggcgagcgcg gcgcgtgcct cctcgtaccc cacgaccagc caggtctcgt gttccggttc
     1921 ggcgcccggg ggacgcacgc ggtgcacggg tccgcgggcg cgcaactcgg cgtagaccgg
     1981 gtgcgggtcg gtgcggaacg cgtctccgta ccgccccagg tcgatcacct cggtcatgct
     2041 gtcctccccc tcgcgacgac tctcacgtcc cctgacggct cccacgtccc ctccgctcaa
     2101 cggacggccc gcccgttcag tgcccgtccg ggagatcttc ctcggtcagg cccgcgtcgt
     2161 aggcgagaag ggcgatctgc acgcggttgt tgagaccgag cttggcgagc acgcgcgaga
     2221 cgtgcgcctt gacggtggcg acgctcatga acagcgtcgc ggcgatctcg gcgttggaca
     2281 gcccgcggcc gacggccacg gcgacctcgc gttcccggtc gttgaggagg gcgaggcgtg
     2341 cacgcgcgcg cgtgcggcgg gtgtcgcccg cggtgccggc cgcgtgcgcc atcaactgcc
     2401 gggtgaccgc gggcgacagc acggggtcac cggccgcgac cgcgcggacc gccgccacga
     2461 tctcggcggg cggggtgtcc ttcaggacga acccggcggc gcccgcgcgc agggcccgca
     2521 gcacctgctc gtcggcgtgg aaggtggtga ggacgacgac ctgcggggcg tccgggcggg
     2581 cgcgcagccg ctcggtggcc gtgagcccgt ccaccgcggg catccggatg tccatcagga
     2641 cgacgtcggg gcgggtgcgg tcgacgagcg cctcgacctg gtcgccgtcg ccggcctcgc
     2701 cgacgatccg gatgtcgtcg gcgccgccca tcatcagcga gagtccggcc ctcacgagcg
     2761 ggtcgtcgtc gacgaggagc actctgatcg cagcagtcat gggcttacga aaccacggcg
     2821 gcggacgcgg gacggctgcc tcacggctgc ctcctccgcc agggccctgg ggcgtgtgcc
     2881 gtgacgctcg ccgtctccgt tcagccccac ggcagccatg cccgcaccgt gaaccccccg
     2941 tcggcgcccg gtccgtgatc cagtcggccg cccgccaggg tggcgcgttc ggtgaggccg
     3001 atcaggccct ggccggagcc ggggacgggc ggggcgggcc ggtcgggcgg cgggttgtgg
     3061 acggtgacgg acaggccttc cccgcgggcg ccggtgacgg tcacggtgac ctcggcgccg
     3121 ggggcgtgct tgcgggcgtt ggtcagcgct tcctggacga cgcggtagac ggtgcggccg
     3181 gccgaggcgg gcaccgcgtc ggggtcggtg acccgctggt cgagggtgac cttcatgccg
     3241 gccgcaccgg actcggcgac gagcgcgtcc agcgcggcga gcgtcggctg ggggcggccc
     3301 gtgtcgtcgc ggtcgcccgc gcgcagcacg ccgatgatct cccgcaggtc ctgcagggcc
     3361 tcgtgggcgc tctcccggat cacgccggcc gcgcgggcga tctcctcgcg gggcgcgtcg
     3421 ggccggaact ccagggcgcc cgcgtgcacg ctgagcaggg tcagccggtg ggcgagcacg
     3481 tcgtgcatct cgcgggcgat gtcctcgcgg gcgagccgct gggcctgttc ggcgcgcagc
     3541 cgcgcctcgg tctcggcccg ccgggcacgg tcgcgcaggc tgagcagcag ctgccgtttg
     3601 gagcggacga acatgcccca gccgatgacg gcgccggtca ggacgaccac gaaggtgacg
     3661 gacaccaggt agggcatctc ggggtcgggc cgccaccagt agtacagcgt ggtcagggcg
     3721 agctgggcgc cgccgatcca ggcgacgtac cggaagggcc ggtgcacggc gagggtgaac
     3781 agcgcgatca ggcaggcgcc gcccgccgtc tcggagacga agttcaccgc ggtcagcgcg
     3841 acggcgaggg cgacgggcag acggcggcgc agccacaccg cgcagcaggc gagcgcgccg
     3901 agcgtctggt ccgtgacggc gagggcgtgc gggatgtgcg ggttgtcgtg caggggttgc
     3961 gcggcggtga acccgacgag cagggccagc aggaagcagg agcagtcgac gacccagtcg
     4021 cgggcggtgc gcctggggcg cccgccgcgc ccggcgtcca ggtcgagttc gagtgcgtgg
     4081 gccagcgcgg agggcagcca ccaccgccgc ccggcgagca ccggcgccgg ggccgggacc
     4141 gtaccgtcgt ccttcacagt cggccaatct acgcagctcc ggacggcgtc acccgccgct
     4201 gcgcgcgctc gtcgaccgaa gtcgcacggg tgtcgactta ggtctgcccg gagccggcgg
     4261 aaggcgggtc ggcccgggtc agatggccat cgcctcctgc agccaccggt tcacccggta
     4321 cggcagccac caggcgaggt cgcccacgcg caggaccagg tgttccccgg tcaggtcggc
     4381 gaggacgggc gggggtatct cgtggtggcc ggtgccggcg tccagggcgg cgatggcggc
     4441 gtggtaggcc ggttcgtccg tggtgaagcg gcgcagctcg ccccagcggc ggtcgcggat
     4501 ctggacgaag ccggggccct gccgccacac gaacttgcac aggtagtggt gggtgcgcca
     4561 ggcgtagagg gcggcgtccg cctcggggag cccgtcgatg cggcgcggcg gatgcaggtg
     4621 ggacagcagc ttccactgct cggcgccgcc gtcggcgggc agccgcaggt cccagtcgac
     4681 ctgtacggcg cgggcggtca gctcccggac caggcagagc aggtcgacgg tccggcgcgc
     4741 ggcggcgggg ccgtcggcgg acaggtcgac gggctcgtgg agggcgacgc ggcgggcgcc
     4801 gagacgccac agaccgtcgc aggtctcgcc ggtcccgccg gtctccgccg gcaggtcgat
     4861 cgtgccgagg gccatgcccg gcagggtgcg cgcctcgtcg tcgaggtcgc gccacgcggt
     4921 cacggagatc cgcgcggcgg ccggcttctc ggcggtctgc gtcatcatgt gcgctccagt
     4981 gctcgcgaag gggtcggggg tcaggacagg gccggttctt ccgcgcgggc cgccggcccg
     5041 tcggcccgca cccggtgggt gtggcgcatg aagtccagcc gcagcaggtc ctggttgacc
     5101 gcggacggcg cgaggtgcac gtactgcccg gcgtccgtga acacgatccc gtcgtccacc
     5161 cagccgtcga ggagcgcccg cacctcgtgt tcgccgcggg gctcggaggt ctcctcggcc
     5221 gccttgcgcc gcagggcggc gacggagtgc ggctggtcca gcagccggaa gacggcgatc
     5281 tcgtacgggt ccgtcagctc acgggtgcgc cacgcgaagc cgctgcgccg gctcaccagc
     5341 acgatccgct cgcccaggtc catgtgggtc agccgggcgt cgacatgccg cttccgccac
     5401 agcgccagcg ccgcgttcag cgcctcgacg gtgtcctcgc cgatgccccg cgcgggtgcc
     5461 tcgaagacgt acgcgaggtc gtacagctcc tcctcgggca ggtcgtaggt gaagcggtag
     5521 tgctgctcgg ggcgcaggcc cgtgaagccc agttcgggct tgtcgaagta ggggctgaag
     5581 cgctcgatgg cgatgcgggc cgacaggtcg accggcgggt tgaggtgttc gagggccggg
     5641 atctggcgga tgacgggcgc gtagtcgtcg tccgtctcgc cggggaagcc gtgcaggtag
     5701 ttccaggaga cggacagccc ggtctcggcg gcgtcccgca gcatgcgcac gttctggcag
     5761 ccgctcacgc ccttgtccat caggtccagg acccggctgt tgaggctctc gatgcccggc
     5821 tggacgtaga tgaggcccgc gtcggcgagg gtcttcagct gcgcgcggcg catgttggcc
     5881 ttgatctcga tgtgcatgcg caggtcgtag ccgctgtcga tgatgcgggg cagcaccgtg
     5941 gagaggtact tcatgtcgag gatgttgtcg acgacgtaca tgtcgagcac gcggtgccgg
     6001 gccgcgaggt ccatgatctc gtcgtagaag gtctcggggc tcttgctgcg gaactgcatg
     6061 aaggagccgt tcagtccgca gaacgtgcag tggtgcttct ccccccacca gcagccgcgc
     6121 gcgccctcca cgaccagttt gggctccacc cagttgcgtg ccacggactt cgccagccgc
     6181 tcgaagtagc cgctgtagtc gggcggcagg atggccgccg gcggcagcgg gctggtcgcc
     6241 atcgggttgg cgacgctgct tccgtcgggc gcgcggtggc acagcccggg tacggcggtc
     6301 aggtcgccgg taccgtcacg cagggcggtc agcagcgcgg ggaaggcgct ctcgccctcg
     6361 ccgcgcacga cgtggtcgac gaaggggaag ttgcggtgca ccgcggcgcc ctgctcggag
     6421 tcgcagttgg cgccgcccat gaccgtcacg atgtgcggtg cgagcgcctt gacgtgccgg
     6481 gcggcggcga gggcggcggt gttctgctgg aaggtggagg tgaagccgac cacgtccggc
     6541 tccgccgcga cgatcgcccg cgcgatctcc tcgacgaact ccggtacgac ccggtgcagt
     6601 tccttcgtca tccgcatccg cgacctgcgc agcttcggcg tcatcacccg ggtgaactcc
     6661 tcgtccttcc actccgggtc gtcgtagagc gcggaggaga acacccagtc gccgcagccg
     6721 aggaagtagg aggacagcgc gtagtactcg tagtcctgcg cggtgaactc ggtgcggcgg
     6781 gtgatccagt cggtgaactc caggttggcg tgcagcacgt cgcaggtggc gtccggcacg
     6841 cgctcgtcga cgctgcgctt gaggatgccg agggccagcg agggcaggtc gatcggggac
     6901 cagggcatgt tgaccagcag aacgcgcacg gcttgctcct tgaaacggga cggcggggag
     6961 acggggaggg gcggagcccg gccggccccg tgcgcggggc cggccgggtg gtgatcagtc
     7021 ctcgtcgccc tcgtcggcgt tccggaacgg aacggtgatg gtgatgccga tgccgggctt
     7081 gcggttctcc tcctcgtccg cgagcggatc gttgtggatg atgtccttct gcacggtgct
     7141 ctcccttcgt tcagtgggtg gttcgtgccg gcccgggccg ccgagtgagc ggcggctccg
     7201 ggctcccccg ttggccgggg gagtcggggg tgaccgggcc cgggggcgcg gtcagtcggc
     7261 ggctcaggaa cgtcatcgcg cgccgcagta cggcgacatg ggcggcgccg tcgtatccgt
     7321 cgtggccggt gtcgagccac atcgcctcgt gcgggacgcc ggcggcgtcg agggcgctga
     7381 ggtaggaacg gatctggtcc ggggggcact tggtgtcccg ggtggcgccg accacgagca
     7441 gcggcgcggt gacgcggggc gcgtagctga gcggtgagct gcgcgcgtag cgttcgggca
     7501 cctcgtccgg ggtgccgccg aacagccgca ggtccagggc gcgcagcgcg gacgtcgagg
     7561 agcggtgcgc ggcggcccag tcggcgagcg gcttcacggc gatgcccgcc tgccacagac
     7621 cggggcgggt gcccagggcg aggagcacca ggtacgcccc ccaggacacg ccccacaggg
     7681 ccgtcgcgtc gggtgcggcg agcccgcggc gcagcaggtc ggcgcggacg gcggcgaggt
     7741 cctggacctg ggtgtggccg actccggcgg tgaaggcgcg ccgccagcgg gggccgtagc
     7801 cggtggagcc gcggtagttg acgcgggcca cggagtaacc ggaggcgacg agggagtgga
     7861 cggtggcgtc gtaggcgtcg cggtcctggt cggcggggcc gccgtggacg aggaagacga
     7921 ggggcgcggg ccggccgggg gcggcggtgc cggggaggct gagcagggtg tggacggggc
     7981 cgtcgggcgt gggggtccac aggtcctggt ggtggccggg cacgtgcggc ggggtgccgg
     8041 tcaccggcgg cagcggcgtg tcgtcggtgg cgcgcagcac cggcgggtgc gcggcgtcgg
     8101 tccacaggta gtgcaggccg ccgccggggc ggggcgcggc gtccaggatc gtgccgggcg
     8161 gtgtggggac gcgggtgcgg cggcgggccg tcaggtcgac gcggtgcagc agggagcggc
     8221 cgtggcggtc ctgccgtacg aggaccgcgc ggctgtccgg gtaccagcgg gcggtgatct
     8281 ccgtgtcgaa cgcgcaccag tcgtggacgg tcgtcccggc ggccggggtc caggtggcga
     8341 ggaggtagtg gtcgtcggtc tggtgcacga ggagcagttc gcgcccgccg tccgggcgga
     8401 agccgcggca ccactgacgg gcggctccgg ccggggatac ggccgtgacg gcgccggccg
     8461 ggtcgagcag cgtgacggcg ccgtccgcgc cggtcacggc gagcaggccg ccgtcggggg
     8521 cgatgccgtg cagggtgagc ggggcgggag cggtgaggag ccgccgcgcg gggtgtccct
     8581 tgcgtccgag gtggacggtg gcggtgcggc cgtcggagag ggcgacggcc gtggtgccgt
     8641 cggcggcgaa ggcgaggccg caggggaggc cgggcgggag gccgggcagg cccggggagt
     8701 ccgggagtcc gcccgctaat tcgccggaga agggccggtg gcgccaggtg ccgtggccgt
     8761 ccgggtcctc gtcgaaccac cagatgccgg catcgtggtc gatggcgcag tgaaacgttc
     8821 cggcgggtcg ggaggtcacc tgccgcgccc ggcgggacgt ggcctcccag gagaagacct
     8881 cgcagcggcc gtccgcgtcc gccgtgaaca ccatgcgggc ggggtcgcgg ggacaggcct
     8941 cgggtaacgc gctgcggaag acctggtagc ggggggcggt gtcgctcatg ggcgttcgtc
     9001 ctctcccgcg gacgggcgtg cggccggcgc cggctggtcg gtacgggggc gcgcgtccgt
     9061 ctcctcggca cgcggacggg cgtccgcctc cgcggtcagc gaccggcccg ccgcgccctg
     9121 cgtgtacacg gcgagcaggg ccagcagcgc caggcacagc acggccgggg tgtgcgggcc
     9181 ccgccacgtc accaggcccg cggcgagcgc cgggcagccg gccgccgcca gcgcggccgc
     9241 ggcgccgacc gccgcgcccg cctgcgactg gacctcgcgc ggtacggccc gcaccaggac
     9301 accggacagg acgacggtga ccgtcggcag cagcagcgcc agcagggcga agcccgtggc
     9361 gtagctcgcg gccgcgtccg cggtgaccag gacgccggcc gggacgagca gcagccaggt
     9421 cgccaggacg acggcgcgcc gcgcgcccag gcggatcacg agccccggcg cgagcagggc
     9481 gcccagcagg ccggccgctc cggaggcgct gaggacgagg ccgacggtgg tgccgccgtc
     9541 gagcacgaac atcgcggtgt acgacagcag cgtcaccagc accgaggcgg ccgacgccca
     9601 caccaggacc atccgcagcg gggcagagcc gaggaccgtc cggaaaccgg aggtcacggg
     9661 cgttctgcgg cgtccgggcg caacgggggt gtcgagcggt gcgcgcagcg cccggatgcc
     9721 gagcgcggtc gcggcgtagg acagggcgtc ggccaggaac ggcaggggcc gggccgcctc
     9781 gaacagggcg ccgccgaggc cggggccaac gacgagggcc gcctggtcgc cggcctggag
     9841 tccggccgcg ccccgcgtga cgtcctcggg ccgcgccaca tgggccagcg ctccccgggc
     9901 cgccgcttcg tgggcgaagg cgcacagccg ctcggtgaag gcgaggccga ggacggccca
     9961 caagggcgga cgccaccaga acagggcggc ggtcagcccg ctcatcgcgg cggcggcgag
    10021 tgccgccgag ccgatcagca gggcgcgccg gtgcccgtgg tcggcgggcc gcgccaccac
    10081 gggtccgagg acgaccccgg cgacggcgac ggcgctggcg aaggatcccg ccgcggcggc
    10141 cggatgcccc aggtcgagga tcagcagcgg caggacgatc ccggaggcct gggtgccgag
    10201 gccgtcgatc gcgttggtcc accacaggat ccgcagatcc cgtcggctct tgcggggcgc
    10261 cggtgccggt gatgccggcg acgacggctc gttcacgatc gttcacctcc ttgcgcagcg
    10321 cgtgcgaagc cgacgggccg gtcgaacaac cggcccgagg tggagaaatc gtgtgctgat
    10381 cgtgtgctgt cggtgaaagc gagccggagc ctagccacgg gttcgcgacg cccacaaggg
    10441 tcaggtggac gtggcgggcg ggaccggaga ggcaactttg accaagaaga atctggtcgg
    10501 cgacgcccgt ggcatgccgt tcggcggaca ccgcggcctg ggcgggcggc cggtggaccc
    10561 gtgagcggcg cccgtcgctg ccgcacacct ggcagacgcg cgtcgcacgc ggtccagggc
    10621 acggggcgtg ccgtgaccgc gcccggcacc ggtcaagcgg aatcttcccc aaggggttgc
    10681 gcgaatcgtt cgggagtcat cgactcccgt ccgccgcgca ggagtcggca cggcatccgc
    10741 ccacactccg cccacccggc cattggccga gaatgcgtcc ggcctcgcac gtcgcccgtc
    10801 atccccgcgg ggcggagggg ggcttacggc gccgccgtcc agcgcggtgc ggtgtcggcc
    10861 cacgccctgt cccacgcggc gagttggcgg cgctggacga cgcgggaggt gacgccgtag
    10921 gcggcgatcg aggcggccgg gacgccgagc gcggcgatga tggccgaccc tcggacccgg
    10981 ttgcgtatct gatccggcgt cagaggcggc tcggtgagtt gtccgtcggc gccgacccag
    11041 acgcgcaggg tgctgccctt ggacagggcg ggcggaacgt cggtcgtggc ggtgcggggg
    11101 cggccctcgg ggtcggtgta gcggacctgt gccgggtaac gggtgccgcg cgcttcggcg
    11161 gagcccggtt ccgggtggtg ccgggcgctc tccaggaggg tcgcggtggt cgcgtggcgg
    11221 gtctgcgcct gccgccgggc ggtttgggtg gcacggtcgt gggccgcgtc ggccgccagc
    11281 accacggctg ccggtgtggc ggccgacgcg gtcaggagca gggcgaggcc gatccagccc
    11341 tggaccaggt cgctgcggcg gcgcagcggg ttgcgccgca gccgccacga caggacccgg
    11401 ggcggcctcc tggcgggcgg gggtgggggt acggcggtgc ggccgggcac gggtcctcct
    11461 tccggcgacc gacgcggcgg gtgggggtcg ggggtggggg tgaggggtcg ggggtggagg
    11521 tagggccgag gacgggccgg ccgagcggga ccgacgcggc aaggcggggt gggccgagga
    11581 cggcccggct cggtgacgcc gacgcgacaa ggcggggtgg gccaagcacg ggccggctca
    11641 atgacaccga cgaggcacgg cggggtgggc agagacggcc cggccggatg acgccgacga
    11701 ggcaccgcgg cggccctgct cagtggaggt cggccgccgg cgggaggccg ccggaggcgg
    11761 aggggtgcgt gtgggcgacc tggaggagcc gggcggcgcc gcggacggcg gcggtgtgcg
    11821 gggccgggac cacggacagg ggcgtgtgca gcaacccggc cagccggccg gtgatctcgg
    11881 ggcgcagggc gcctcccccg gccagcagga cgccgcgggc cagcgcgtc
//
