LOCUS       NZ_BNBM01000014        21041 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces lanatus strain JCM 4588 sequence14, whole genome
            shotgun sequence.
ACCESSION   NZ_BNBM01000014
VERSION     NZ_BNBM01000014.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces lanatus
  ORGANISM  Streptomyces lanatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 281063)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 61X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014655715.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 05:46:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,254
            CDSs (total)                      :: 9,172
            Genes (coding)                    :: 8,973
            CDSs (with protein)               :: 8,973
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 83 of 199
            Pseudo Genes (incomplete)         :: 153 of 199
            Pseudo Genes (internal stop)      :: 16 of 199
            Pseudo Genes (multiple problems)  :: 50 of 199
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:57:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 222413
            Orig. end                         :: 243454
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNBM01000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21041
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21041
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21041
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11041](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11041
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(883..3043)
                     /gene="helR"
                     /locus_tag="IE325_RS31660"
                     /old_locus_tag="GCM10018780_64000"
                     /pseudo=""
     CDS             complement(883..3043)
                     /codon_start=1
                     /gene="helR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121680.1"
                     /locus_tag="IE325_RS31660"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64000"
                     /product="RNA polymerase recycling motor ATPase HelR"
                     /pseudo=""
                     /transl_table=11
                     /translation="MFDLPERLSPKAEPTLIAGDERHFAAIADCLEKTITELSDRLAAA
                     RRAPGGLGREAMDRDSEIHRLTARLRTLRRFGLDLCLGHGRRRWHRARVRRTARPDRRH
                     GPPTAHRLALPRGRAVLRRHPRQSDGPGQPPQVPLDRRTDQRLLGRGVHPGRLRRACRA
                     RRPVRVHRRPGRQPLTSDAGRARHHPGRPGRRHPRGIPGRTRRRRRTRHGQDSRGPAPL
                     RLSAPLRPPRRPPPGRRAVRRPAPAVSGVRLRCPAQPRRGGRADLRARGPGSGGCHGEG
                     RGRSGGGPSEVVRGPGRGDREGRPVLRGAAREGYDGHDSLVRRPVERRRLGRGVRGGRT
                     RYAAQRGPRPGLGGAAHDPGRQARGGRLGRRRRGPGRAGPQVAHPEQGTADGLQPCVAA
                     PRCHGPGLGPVVGARVSADVRALARPRGGPAVAAGGRAGLDGVRPAAPGRGPAPARGPG
                     RGPAQTPAGGDPRRAARADDAGRRQPDRGHRRLRRRHRRGRRAGDHAAGPGRPGQPGRR
                     VRTADRRPRPARRPVRAHRGGRGAGTDRRRVADAAAALPVPELHHRGRPRPGPARLHRV
                     LARTPRTGRPRPDRPGLPEHQLPHTGRDHGGGRAGDPRRAAGRQCADVHPQRRGAGRAR
                     FRFGPCLDPGHLARRACGGSRLRHRRPHVPPDGPGAVTDAGAVEGPGVRPGRPGEPGGV
                     RPGHRGSGGPLCGDDQGDPATGHPCV"
     misc_feature    complement(929..931)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3260..3273
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.49"
                     /tool="antismash"
     misc_feature    complement(3261..3274)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.87"
                     /tool="antismash"
     misc_feature    3461..3475
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.54"
                     /tool="antismash"
     gene            3546..5138
                     /locus_tag="IE325_RS31665"
                     /old_locus_tag="GCM10018780_64020"
     CDS             3546..5138
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007447816.1"
                     /locus_tag="IE325_RS31665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64020"
                     /product="sensor histidine kinase KdpD"
                     /protein_id="WP_190073547.1"
                     /transl_table=11
                     /translation="MVSVQSPPSRRELPYARVLLLPAIVMAAATGAAVAVVAEPARTAV
                     GVCGAVAVLLVMASAAEAVRRGRLLRDLRADHARHTAYMERRIAAHDEEMSRLATEITP
                     AAIHRLQCGSSAYEVMRDLADIDPSYAHLSRAQLALVESMLAIVDHEEALRDSSQRSFV
                     NIARRVQSIVHQQAVELREMEEDHGRNPEVFDDLLRIDHGTMLIGRLADSISVLGGGRP
                     GRQWPEPVPLYSVLRGAMSRILEYRRISLDSIVHVNIAGISVEPIIHAAAELLDNATRY
                     SPPQTKVHVTATEVQTGIAIEIEDSGVSLSEEARARVEGMLERAKQGTDLQDLGEAPRL
                     GLAVVGRLCTAFNMQVALRTSAYGGVRAVLVVPREMMTTDPAPGLAHGVGAAAAPKADA
                     DALEGPKRAPKKRRPTSPKIPTTVSMEDDVPVVTEWTAGGLPQRRSRVKTPLSKRIAEQ
                     AAIEQAEREGRPTVWSTRRPEPEPEPEKDDEPAPGAWVEAFWNGLKDKPDPYTLQPRTQ
                     PARPEADDEGDIK"
     gene            5135..5542
                     /locus_tag="IE325_RS31670"
                     /old_locus_tag="GCM10018780_64030"
     CDS             5135..5542
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018549147.1"
                     /locus_tag="IE325_RS31670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64030"
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="WP_062722698.1"
                     /transl_table=11
                     /translation="MIQQRANFDWMLKELHDGVPGIEMIVVLSADGLRIARYGGDPDTA
                     DRVAAACAGLQSLASAVATEIPNSDGKMKMVLIEINGGYFYLMAAGANAYLAVLSDTFA
                     EPGRMSNRMSDLVARIGAHLTSPPRRNGQTV"
     gene            5539..5940
                     /locus_tag="IE325_RS31675"
                     /old_locus_tag="GCM10018780_64040"
     CDS             5539..5940
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003977198.1"
                     /locus_tag="IE325_RS31675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64040"
                     /product="DUF742 domain-containing protein"
                     /protein_id="WP_190073548.1"
                     /transl_table=11
                     /translation="MTPPKRQRRRPKEKPAPPPPQTGKQKNPERLFVVAGPDGGERDGD
                     LDLVTLIVARADPPPSPTPEQSALLRLCTAPLSVAELSAYLNLPFSMMTVLLTDMLTAG
                     LVTARNPIVRQTVADRSILEAVMHGLQRL"
     misc_feature    5689..5691
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5921..6541
                     /locus_tag="IE325_RS31680"
                     /old_locus_tag="GCM10018780_64050"
     CDS             5921..6541
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016434372.1"
                     /locus_tag="IE325_RS31680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64050"
                     /product="ATP/GTP-binding protein"
                     /protein_id="WP_190073549.1"
                     /transl_table=11
                     /translation="MDFKGSDTVPGPRTEDQLPHTAEAAVKIVIVGGFGVGKTTMVGSV
                     SEIRPLTTEETMTQAGIGVDDNFGSDTKTATTVAMDFGRISITEQLVLYLFGTPGQERF
                     WFLWNGLFDGALGAVVLIDTRRLEVSFDVMGRLEERGVPFVVAVNTFPDAPRYPVDELR
                     TALDLPEEIPIIECDVRRRASSRDVLMTLLQFLHALAMAKAPA"
     gene            6707..8176
                     /locus_tag="IE325_RS31685"
                     /old_locus_tag="GCM10018780_64060"
     CDS             6707..8176
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380986.1"
                     /locus_tag="IE325_RS31685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64060"
                     /product="cytochrome P450"
                     /protein_id="WP_190073550.1"
                     /transl_table=11
                     /translation="MTPEPLSPIGTDEPTATPPPGCPAHGIGPDGLRRLHGKEAEDLGA
                     VYEELRAEHGPVAPALLHDDVPVWVVLGHTENMQMLRTPSQFCRDSRLWTAVQDGTLKP
                     DNPLMPHIAWQPICCHAEGDEHVRLRGAVTDAMEAIDHRGVRRYINRATQHLVNKFCET
                     GRADLVSQFAEHLPMVVMCEILGMPDEYNDRLVQAARDLPKGTETAIASNAYVMDVLMR
                     LTAARRAESKDDFASRLVDHPAGLTDDEVGQHLRVVLMAAYEATANLLANVLRVVLTDP
                     RFRAQLSGGQMTVPQAVEQSLWDEPPLSSVLCYYAKQDTELGAQRIRKGDGLILGIAPG
                     NVDPRVRPDLKANMKGNRSHLAFSGGPHECPGQDIGRAIADVGVDALLSRLPDLQLACE
                     EDELRWQASIFNRHLVELPVKFAPKPQQDAMAGPAHPRPPQRPSNGQVGSPQPQTATPE
                     PATPAAAPTRTPEPASRPGLFRRLLRWWRGD"
     gene            complement(8195..9136)
                     /locus_tag="IE325_RS31690"
                     /old_locus_tag="GCM10018780_64070"
     CDS             complement(8195..9136)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1279: pseudouridine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007447831.1"
                     /locus_tag="IE325_RS31690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64070"
                     /product="RluA family pseudouridine synthase"
                     /protein_id="WP_190073551.1"
                     /transl_table=11
                     /translation="MRRRSPPPPSPLPQREGVDPVRVRLPAGEAWATVRDHLVARLAAG
                     AEVIDAMLDAGEIVDVAGRPVPRDMAYTPGMYLWFQRKLPDEERVPFTVDVVYQDEHVV
                     VADKPHFLATTPRGSHVAETALARLRRQLDVPTLGAAHRLDRLTAGLVLFTVRPEERGA
                     YQSLFRDKRVTKEYEAVALYDPALDLPRTMRSRIVKERGVMAAREVEGEPNAVSRVELL
                     EHLEGRARYRLVPHTGQTHQLRVHMSALGMPILGDPLYPVVTDPVASGDFRHPLQLLAR
                     VLEFTDPITGCEHRFVSRRVLGAWASYDEWAG"
     gene            10001..11041
                     /locus_tag="IE325_RS46715"
                     /old_locus_tag="GCM10018780_64080"
     CDS             10001..11041
                     /GO_function="GO:0010333 - terpene synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF040195.4"
                     /locus_tag="IE325_RS46715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64080"
                     /product="terpene synthase family protein"
                     /protein_id="WP_190073552.1"
                     /sec_met_domain="T1TS (E-value: 2.3e-54, bitscore: 179.4,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPFRIPEFDMPIASSGLSPAMDAVEPDMWAWAAAHQLVRGEAMR
                     RHLARTRPQWATAIYLPGADEAHLRVPNRFMIWAFILDDNLDDSISEGHIESVTYLLDE
                     LVAVCRNLNTPVTNEGRALKEILDDLSQGRTRAWRNVLGESVVRWLNTFLPEARASRAG
                     RVMTIDEYLPHRRNGVCEFIFAHLQEYVRDVELPKGVRDLPAMEQARNLACEWTGLYND
                     IYSVEKEESVGYLHNAVLVARTHRGCSTQEAVDIVAAITNSLLRQFLAACDAAPAQIRA
                     AADYTPTVLAHASDVIDGYRDLVRANYDYHRGAARYSDVAKYMPEAITTSGLRPDWSFT
                     PTITHS"
     gene            complement(11326..12171)
                     /locus_tag="IE325_RS31700"
                     /old_locus_tag="GCM10018780_64090"
     CDS             complement(11326..12171)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1200: vibriobactin
                     utilization protein ViuB"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501125.1"
                     /locus_tag="IE325_RS31700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64090"
                     /product="siderophore-interacting protein"
                     /protein_id="WP_190073553.1"
                     /transl_table=11
                     /translation="MALRPARKPRKPHSAQVVRTERLTPHMQRVVLGGDGLAEFAADTC
                     TDHYVKMLFSPAGVSYPEPFDLERIREELPREQWPVTRTYTVRAWDPEHRELTLDFVLH
                     GDEGLAGPWAMRAQPGETVRFMGPGGAYAPDPDADWHLLVGDESALPAIARSLESLPDG
                     ATAYAFVEIAGPEEEQKIDSDVEVIWLHRGSRRVGEALLEAVQTLEFPEGRVHAFVHGE
                     AHFVKELRHLLRVERGVAREDLSISGYWRLGHDEDGWQASKRDWNARIEAEQEGAAPAA
                     "
     misc_feature    12190..12208
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.56"
                     /tool="antismash"
     misc_feature    12191..12211
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    complement(12315..12330)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     gene            complement(12343..13275)
                     /locus_tag="IE325_RS31705"
                     /old_locus_tag="GCM10018780_64100"
     CDS             complement(12343..13275)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045305.1"
                     /locus_tag="IE325_RS31705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64100"
                     /product="5'-3' exonuclease"
                     /protein_id="WP_190073600.1"
                     /transl_table=11
                     /translation="MMRGVTGRLMLLDTASLYFRAYFGVPDSVKAPDGTPVNAVRGLLD
                     FIDRLVKDHRPDALVACMDADWRPEWRVELIPSYKAHRVAEEHEVGPDEEEVPDTLSPQ
                     VPVIEAVLDALGIARVGVAGYEADDVIGTFTARAKGPVDIVTGDRDLYQLVDDERGVRV
                     LYPLKGVGTLQLTDEAWLREKYGVDGGGYADLALLRGDPSDGLPGVPGIGEKTAAKLLA
                     EFGDLAGIMAAVDDPKAKLTPSQRKRLDESRPYVAVAPKVVRVALDVPLSDVDTTLPRA
                     PRDAAGLDELAARWGLGGSLQRLLVTLGA"
     misc_feature    complement(13336..13351)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.11"
                     /tool="antismash"
     gene            13462..14538
                     /locus_tag="IE325_RS31710"
                     /old_locus_tag="GCM10018780_64110"
     CDS             13462..14538
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501129.1"
                     /locus_tag="IE325_RS31710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64110"
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="WP_190073554.1"
                     /transl_table=11
                     /translation="MSSRLEADHLYKVFGRRPNDAVERLRKGADREELRADGTTAAVID
                     ASFTVEPGQIFVVMGLSGSGKSTLLRMLNGLLEPTAGHVRFDGQDLTAIGDRELREVRA
                     KKISMVFQHFALFPHRSVLENAAYGLAVQGVPRAEREKRAAEALELCGLGGWEKSWPDE
                     LSGGMQQRVGLARALATDADLLLMDESFSALDPLIRRDMQDQLLQLQKTLKKTIVFITH
                     DLNEAMRLGDRIAVMRDGRIVQTGTAEDILLRPADDYVASFIQDVDRSRVLTAGSLMDT
                     SVTADAPLCTCETATTETTFVELCAISARLSHRVTVVDDDNKVIGVVPRQRLVGFLGDE
                     SADPSPCDNPEGKVTARA"
     gene            14531..16357
                     /locus_tag="IE325_RS31715"
                     /old_locus_tag="GCM10018780_64120"
     CDS             14531..16357
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671663.1"
                     /locus_tag="IE325_RS31715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64120"
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="WP_190073555.1"
                     /transl_table=11
                     /translation="MPRLRLGDWVDSGVDWLVAHMGWLFDAIKAVVEGMYDGVNAVLTA
                     PEPLLLGGILAVLAWWLRGLVPGVLAFGGFALIDSLDLWDRAMSTLALVLVATVIALVI
                     SVPLGIWAARSKTVSAAVRPVLDLLQTMPSMVLLIPAILFFGLGTAAGVIATLIFALAP
                     GVRMTELGIRQVDAELVEAAEAFGTTPRDTLLRVQLPLALPTIMAGINQVIMLSLSMVV
                     IAGMVGTGGLGGAVNEAIGQLDIGFGFEAGVGIVVLAIYLDRITGALGAQISPLGRRAA
                     AKARAAGGVKVWSHRPRPVVAVAGVAALALVAGGMGVFGPTSGTAAASATDVGKGKEIK
                     LGYIPWDEGIASTFLWKELLEQRGFKVTTSQYAAGPLYTGLATGQIDFETDSWLPTTHA
                     EYWEKYGKQLEDLGSWYGPTSLELSVPSYVKGVDSLEDLKSNASKFKGKIVGIEPSAGM
                     MGMLKDKVLKEYGLEGSYDVVDGSTPAMLAELKRAYAKKEPIVVTLWSPHWAYSDYDLK
                     KLKDPKGAWGKGDGVHTLARKGFAGDNPEVGQWLKNFSMTEKQLTGLESQITKAGKGKE
                     QDAVRTWLKQNPGLLDKWAPIDASDLKNQAAG"
     gene            16875..17444
                     /locus_tag="IE325_RS31720"
                     /old_locus_tag="GCM10018780_64130"
     CDS             16875..17444
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501133.1"
                     /locus_tag="IE325_RS31720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64130"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_190073556.1"
                     /transl_table=11
                     /translation="MGDHKEQPLRVGAAVRRRRRALDLTLAVVAERSGLSVPFLSQVEN
                     DRARPSRSSLEKVADALRTTAVELLAAADPACSVDVVRAEGNELGPEPRVRSLVRGHHQ
                     MHASEFTGDHDAGREFQYRNDQLMYVADGAVEIEAEGRAYRLGRGDSLYLTGGVRHRWR
                     ATVPDTRVVVVAVAEHIEAVRNRPGR"
     gene            17444..18154
                     /locus_tag="IE325_RS31725"
                     /old_locus_tag="GCM10018780_64140"
     CDS             17444..18154
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117683.1"
                     /locus_tag="IE325_RS31725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64140"
                     /product="helical backbone metal receptor"
                     /protein_id="WP_190073557.1"
                     /transl_table=11
                     /translation="MRVVSLVPSLTEAVADLGVLVGATDWCSHPADLDVTRVGGTKNPK
                     VDRVLALAPDLVIANEEENREPDLAALRAAGVDVLVTEVRDVPQAFGELARVLEACGVT
                     ARPRWLDEAEDAWSSPAPDRRTTAVVPIWRRPWMVLGRDTFAGDVLARLGVDHVYTTHE
                     DRYPRIPLDDLREAAPDLVVLPDEPYRFTADDGPEAFPGLPCALVSGRHLTWYGPSLAE
                     APRVLTAALRAAHR"
     gene            complement(18066..19223)
                     /locus_tag="IE325_RS31730"
                     /old_locus_tag="GCM10018780_64150"
     CDS             complement(18066..19223)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661678.1"
                     /locus_tag="IE325_RS31730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64150"
                     /product="TDT family transporter"
                     /protein_id="WP_190073558.1"
                     /transl_table=11
                     /translation="MVTAARHLGPTRRLGVRHLGPNWYAAVMGTAAVATAGAGLPLHLP
                     GLRTVCAAVWALSLALLVTLLGARAAHWSHHRDQARAHLLDPAMAPFYGCLSMALLAVG
                     GGALVVGRDWIGTSAAVALDAVLFAAGTAIGLAAAVAVPYLMAVRHRVEPSEATPVWLL
                     PLVAPMVSAALGPLLVPHLPAGQARETLLLACVAMFGLSLLATLLMLPLVFARLITGGP
                     LPLVLTPSLFLVLGPLGQSTTAVGVFADVAPGVVPAPYSEGFGMLAVLYGVPVMGFALL
                     WFGLATAHVLRARRHGMGFAMTWWAFTFPVGTCVTGATALARHTGLVVYEGLAVGLYVV
                     LVAAWGVAAVHTTRGLFSGALLAGPPSVPVVPRQVTGRTTSGAAR"
     gene            19294..20217
                     /locus_tag="IE325_RS31735"
                     /old_locus_tag="GCM10018780_64170"
     CDS             19294..20217
                     /GO_function="GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002559.1"
                     /locus_tag="IE325_RS31735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_64170"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_190073559.1"
                     /transl_table=11
                     /translation="MTEVAEGQFRAGSLSHRVPELGALELLLAVARLGSLGGAARELGI
                     TQPAASSRIRSMERQLGVALVDRSPRGSRLTDAGALVTDWARRIVEAAEAFDAGAQALR
                     DRRDSRLRVAASMTIAEYLLPGWLLALRAQRPDTAVSLLAGNSAAVAERLLADEADLGF
                     VEGVSVPTGLDSVVIAHDNLIVVTAPGHVWARRRRALSGAELAATPLILREEGSGTRQV
                     LDSALGGLARPLIELSSTTAVKAAAVGGAGPAVLSELAVGEELSMRRLVSVPLADVSLA
                     RDLRAVWPTGHRPVGPARDLLALTRG"
     misc_feature    complement(20859..20874)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.67"
                     /tool="antismash"
ORIGIN
        1 cccgcgctcc ccgcagtccc gtcgggacct ggcgtccgcg ctgcgctcca aggccgggca
       61 catcgtgccc gaccggcacc gcaagcgcgg ctcgcaggcg accgacgacc gtgagatcgc
      121 ccggttgcgc aaggcgatcc gcgcccaccc ctgccacggc tgcgacgacc gtgaggacca
      181 cgcccgttgg gccgagcgct atcaccggct gatgcgtgac acctcgcagc tggagcgccg
      241 tatcgaaggc cgcacgaaca cgatcgcgcg caccttcgac cgcatcgtcg cgctgctgac
      301 cgagctggac tatctgcgcg gcgacgaggt caccgagcac ggcaagcggc tcgccaggct
      361 ctacggcgaa ctggacctgc tggccagtga atgcctgcgc gagcgggtgt gggagggcct
      421 cggccccgcc gaactggcgg cctgtgtctc ggcgttggtg tacgaggcac gggtcgggga
      481 cgacgccatg gcgccgaagc tgccctcggg caaggccaag gccgcgctcg gggagatggt
      541 gcggatctgg ggccggttgg acgcgctgga ggaggacttc cggatcaccc agtccgaggg
      601 cgtcgggcag cgcgaacccg atctgggctt cgcctgggcg gcgtacatgt gggccagtgg
      661 caaggggctg gacgaggtgc tccgggaggc tgagatgccg gccggggact ttgtgcggtg
      721 gtgcaagcag gtgatcgacg tgctcgggca gatcgctgcg gcttctccgg tggagggctc
      781 caccgtgacc aagaatgcgc gtaaggctgt ggaagggctg ctgcgggggg ttgtcgccta
      841 ctcttcggtg gggtgaggcg ttcggcgacc ggtggaggtc ggtcacgcaa ggatgaccag
      901 ttgctgggtc gccctggtca tcgccacata acggtccacc gctccctcga tgccctggcc
      961 gaactcctcc gggttcacca ggacgaccag gtcgaactcc aggcccttcg acagctccgg
     1021 cgtcagtgac cgcacccggt ccgtcgggcg gaacgtgggg tcgccgatga cgcaggcgac
     1081 tccctccgca tgctcggcga gccaggtgtc caggatcgag gcaaggtccg aaacggaacc
     1141 gtgcacgacc ggcacccctc cgctgcggat ggacgtcggc acattggcgt ccggcagcgc
     1201 ggcgcggatc accggctcgg cctccgccat gatctcttcc ggtgtgcggt agttgatgtt
     1261 cagggaggcc aggtcgatcc ggtcgaggcc gacccgttcg aggcgttcgc gccaggactc
     1321 ggtgaagccg tgccgggcct gggcgcggtc gcccacgatg gtgaagctcc gggacgggca
     1381 gcgcagcagc agcatctgcc actcggcgtc ggtcagttcc tgcgcctcgt ccaccacgat
     1441 gtgcgcgaac gggccggcga gccggtcgag gtctccggtc ggcagttcgg actcgtcgac
     1501 caggctgacc tgggcgtcct ggccccgcag catgatcacc agcccttcgc cctcgtcggt
     1561 gtcggcgccg gagtcggcgg tggcctcgat caggttgtcg acgacctgcg tcatccgctc
     1621 gcgctgcgcg gcgaggatcc cctcctgccg gtgtctgcgc cgggccgcgt ccgggtcccc
     1681 gagccggtgc cgggccgcgt ccaggagcgg caggtcggac accgtccagg cctgcgcgtc
     1741 cgcccgctgc aaccgctgga cctcctcgcg gccgagccag ggcgcgcaca tccgcagata
     1801 cgcgggcacc gaccacaggt ccgagaccag gtccgtggca tcgaggagcg gccacgcacg
     1861 gttgaaggcc gtcagcagtt ccctgttccg ggtgagcgac ctgcggacca gctcggccgg
     1921 gacctcgccg tcgtcgcccg aggcgtcctc ctcgtgcctg tcgaccagga tcgtgagcag
     1981 ctcctcccag acctggtcgc gggcctcgtt gtgcggcgta ccgggttcgg ccgcctcgaa
     2041 cgccacggcc cagtcgacgc cgctcaaccg gacgtcggac cagggagtcg tgaccgtcat
     2101 acccttcgcg ggcggctcct cgtagaaccg gacggccttc tcgatcgccc cgaccaggtc
     2161 cgcggacgac ttcagacggg ccacctccgg atcggcctcg gccttcgccg tggcaccctc
     2221 cggaaccagg tccgcgagca cgcaggtctg cacgccctcc tcgccgaggc tgggcaggac
     2281 atcggagacg tacgccagat acggccggtg cgggccgacg aacagcacgc cgccccggcg
     2341 gtggtcgacg cgggggtcgg agtggagcag ataggcggag cggtgcaggg ccacgactgt
     2401 cttgcccgtg ccgggtccgc cgtcgacgac gagtgcgccc cgggatcccg cgcggatgac
     2461 ggcgtcctgg tcggcctgga tggtggcgag cacgtcccgc atccgaggtg agcggttgcc
     2521 gcccaggccg gcgatgaacg cggactggtc gtcgagcgcg gcatgcccgg cgaagccgtc
     2581 ctgggtgaac acctcgtccc agtagtcgct gatccgtccg ccggtccagc ggtacctgcg
     2641 gcggctggcc aggcccatcg gattggcgtg ggtggcgccg aagaacggct cggccgcggg
     2701 ggagcgccag tcgatgagca gtcggcggcc cgtgtcgtcg gtcaggccga gccgtccgac
     2761 gtacacgggc tcggtgccat ctgcgccgac catgcccgag gcacaggtcg aggccgaagc
     2821 ggcgcagggt gcgcagccgg gcggtgagcc ggtggatctc gctgtcccgg tccatcgcct
     2881 cccggcccag gccccccggc gccctgcgcg cggcggcgag acggtcggac agctcggtga
     2941 tcgtcttctc caggcagtcg gcgatggccg cgaagtgcct ctcgtcgccg gcgatcagag
     3001 tcggttcggc tttgggggag aggcgttcgg gcaggtcgaa cacgctggtt tcggcttggc
     3061 tcctactgtc actcaactgc acacgcactt ctacgactcc cgtttccgca ggtcctcggc
     3121 ctaggccggt gattgtgcgg tacaacgggg gccttgccgc aagccccccg gtgcgctata
     3181 cgttgagagt ggcgaggagc aggtggctcc ttgcccttct tttttgccta ggtccgggct
     3241 cggaacccga tcttcacccg tcacggtgag tgactttcgc acgtgcgtgc gccgtgacgc
     3301 agtgtattcg aacgtaatct gagcgtataa atggggccga gcgtactcct gtctcgcacc
     3361 ccatttcagg tgcttgctga atatgacaca gcgatgatcc ggtcggacta agctcgcccg
     3421 cagcgcaccg agttgaggtg tattcgtgcg cttgttccca aacatgcaga agattccgac
     3481 atacccatga cgtaccccgc tgcaagctcc ccctacaccc aaccgattcg tttcagaggg
     3541 cccacatggt gagcgttcaa tccccgccga gtcgccgtga actcccgtac gcgcgcgtac
     3601 tgttgctgcc ggccatagtg atggccgcgg cgaccggagc cgccgtcgcc gtggtggcgg
     3661 agccggcccg gacggccgtc ggcgtatgcg gtgccgtggc cgttctgctg gtgatggcct
     3721 ccgcggccga agcggtgcgt cgaggccgcc tcctgagaga cctgcgggcc gaccacgccc
     3781 gtcacaccgc gtacatggag cgccgtatcg ccgcccatga cgaggagatg tcccggctcg
     3841 ccacggagat cacaccggcc gcgatccacc ggctgcaatg cggcagttca gcgtatgagg
     3901 tgatgcgaga cctcgccgac atcgatccgt cctacgccca tctctcccgg gcacagctcg
     3961 cgttggtgga gtcgatgctc gccatcgtcg accacgagga agccctccgc gactcctccc
     4021 agcgctcctt cgtcaacatc gcccgccgtg tccagtcgat cgtccaccaa caggccgtgg
     4081 aactgcggga gatggaggag gaccacggcc gcaaccccga ggtcttcgac gacctgctgc
     4141 gcatcgacca cggcaccatg ctgatcggcc gcctcgccga ctccatctcc gtgctcggcg
     4201 gcggccggcc cggccgccag tggcccgaac ccgtcccgct gtacagcgtg ctgcgcggcg
     4261 ccatgtccag gatcctggag taccgccgca tcagcctgga ctccatcgtc cacgtcaaca
     4321 tcgccggaat ctccgtcgag ccgatcatcc acgccgcggc cgaactcctc gacaacgcca
     4381 cgcgctactc cccgccgcag accaaggtgc acgtcaccgc caccgaggtg cagaccggaa
     4441 tcgcgatcga gatcgaggac tccggcgtca gcctcagcga ggaggcccgc gcccgggtcg
     4501 agggcatgct ggagcgcgcc aagcagggca ccgacctcca ggacctcgga gaggccccac
     4561 gcctgggcct ggccgtcgtg ggccgtctct gcacggcgtt caacatgcag gtcgcgttgc
     4621 gaacctcggc gtacggcggc gtccgcgccg ttctcgtggt gccgcgcgag atgatgacca
     4681 ccgaccccgc gcccggactc gcccacggcg tcggcgccgc ggcggcgccc aaggccgacg
     4741 ccgacgccct cgaaggcccc aagcgtgccc ccaagaagcg tcgccccacc agcccgaaga
     4801 tccccaccac ggtctccatg gaggacgacg tccccgtggt caccgagtgg accgcgggcg
     4861 ggctgccgca gcgccgcagc cgggtgaaga ccccgctcag caagcgcatc gccgagcagg
     4921 cggccatcga gcaggcagag cgggagggca ggcccaccgt ctggtcgacg cgcaggcccg
     4981 agccggagcc cgagccggag aaggacgacg agcccgcccc cggagcatgg gtcgaggcgt
     5041 tctggaacgg actcaaggac aagcccgacc cctacacact ccagccgcga actcaaccgg
     5101 cccgccccga ggccgatgac gagggggaca tcaagtgatc cagcagcgcg ccaacttcga
     5161 ctggatgctc aaggagctcc acgacggcgt acccggcatc gagatgatcg tggtgctctc
     5221 cgccgacggt ctgcgcatcg cccgctacgg cggtgacccg gacaccgccg accgcgtcgc
     5281 tgcggcgtgc gccggtctgc agagcctggc gagcgctgtc gccacggaga tccccaacag
     5341 cgacggcaag atgaagatgg tcctcatcga gatcaatggc ggctacttct atctgatggc
     5401 cgccggcgcc aacgcctatc tcgccgtgct ctccgacacg ttcgccgagc cgggtcggat
     5461 gagcaaccgg atgtccgacc tcgtcgcccg gatcggtgcc catctcacca gtccgccccg
     5521 gcgcaatggg cagaccgtat gactcctccg aaacgccagc ggcgacgtcc caaggagaaa
     5581 ccggcaccac cgccgcccca gacggggaag cagaagaacc cggagcggct cttcgtggtg
     5641 gccggaccgg acggcggcga gcgcgacggc gacctcgacc tcgtgacctt aatcgtggcg
     5701 cgcgccgacc ccccgccgtc accgacgccc gagcagtcgg cgctgctccg gctctgtacc
     5761 gcccctctgt ccgtggccga gctgtcggcc tacctcaacc tgccgttcag catgatgacg
     5821 gtcctgctca ccgacatgct gacggccgga ctggtgacgg cacgcaaccc gatcgtgcgc
     5881 cagaccgtcg ccgaccgttc aatcctcgaa gcggtgatgc atggacttca aaggctctga
     5941 caccgttccc ggtccgcgga ccgaggacca gctgccgcac acggccgagg ccgccgtgaa
     6001 gatcgtgatc gtgggcggct tcggcgtcgg aaagacgacc atggttggct cggtcagcga
     6061 gatcaggccg ctgaccaccg aagagaccat gacgcaggcc ggtatcggcg tcgacgacaa
     6121 cttcggctcg gacaccaaga cggccaccac cgtcgcgatg gacttcggcc gcatcagcat
     6181 caccgagcaa ctggtgctgt atctcttcgg tacgcccgga caggagcgct tctggttcct
     6241 gtggaacggc ctgttcgacg gcgcgctcgg cgcggtcgtg ctgatcgaca cgcgccgcct
     6301 cgaggtcagc ttcgacgtca tggggcggct ggaggagcgc ggtgtgccgt tcgtggtggc
     6361 cgtcaacacc ttcccggacg cgccccgtta ccccgtcgac gaactccgga cggcactcga
     6421 tctgcccgag gagatcccca tcatcgagtg cgacgtacgt cgccgggcct ccagccggga
     6481 cgtattgatg acgttgctgc agttcctgca cgcgctggcc atggcgaagg cgcccgcatg
     6541 atctgcagac cggtcgcacc gcacgtgtcc tgaacatgtg cgcagacggc gcccgccccg
     6601 tctgcacgac ctctgcccgg ttctcggctt cgatcgaatc gcctgaaccg gggcatcccg
     6661 ttacctcttg tacagccgga atcacttcag cttcggagcg accaccgtga cgcctgaacc
     6721 tctctccccg atcggtacgg acgagcccac ggccacccca ccgcctggct gccccgcaca
     6781 cggcattggc cccgacgggc tgcgccgact gcacggcaag gaggcagagg acctgggagc
     6841 cgtgtacgag gaactccgtg ccgagcacgg ccccgtggct cccgccctgt tgcacgatga
     6901 cgtgccggtc tgggtggtgc tcggccacac cgagaacatg cagatgttgc gcacgccctc
     6961 gcagttctgc cgtgacagtc ggttgtggac cgcggtgcag gacggcaccc tgaagcccga
     7021 caacccgctc atgccgcaca tcgcatggca acccatttgc tgccatgccg agggcgacga
     7081 gcatgtgcgg ttgcgtgggg cggtcaccga tgccatggaa gccatcgacc accggggcgt
     7141 ccgccgctac atcaaccgcg cgacccagca cctcgtcaac aagttctgcg agacaggcag
     7201 agccgatctg gtcagccagt tcgccgagca cctgccgatg gtggtcatgt gcgagatcct
     7261 cggtatgccc gacgagtaca acgaccgact cgtgcaggcc gcccgcgact tgcccaaggg
     7321 caccgagacc gcaatcgcca gcaacgcgta cgtcatggac gtcctgatgc ggctcaccgc
     7381 ggcccgccgg gccgaatcca aggacgactt cgccagtcgc ctcgtcgacc acccggccgg
     7441 gctcactgac gacgaggtcg gccagcacct gcgcgtcgtc ctgatggccg catacgaggc
     7501 caccgccaac ctcctcgcca acgttctgcg tgtggtgctc accgacccgc ggttccgagc
     7561 ccagctcagc ggcggtcaga tgacggtgcc ccaggcggtc gagcagtccc tgtgggacga
     7621 gccgccgctc agcagcgtcc tgtgctacta cgccaagcag gacaccgagc tgggtgccca
     7681 gcgcatccgt aagggtgacg gcctcatcct gggcatcgcg ccgggcaacg tcgacccgcg
     7741 ggtccgtccc gacctcaagg ccaacatgaa gggcaaccgt tcccacctgg ccttcagtgg
     7801 cggtccccac gagtgtccgg gccaggacat cggccgcgcc atcgccgatg tgggcgtcga
     7861 cgccttgctg agccgcctcc cggatctcca actcgcctgc gaggaggacg aactgcgctg
     7921 gcaggcgtcc atcttcaacc ggcacctggt ggagctgccg gtgaagtttg cgcccaagcc
     7981 tcagcaggac gccatggcgg gacccgcgca cccacggccg ccgcagcgtc ccagcaacgg
     8041 ccaggtcggt tcgccgcaac cgcagacagc gactccggag ccggccactc cggccgcggc
     8101 cccgacccgg acgccggaac cggcttcccg accgggcctg ttccggcgcc tgctgcgctg
     8161 gtggcgcggc gactgatccg cgccggcacg ggtgctaccc ggcccactcg tcgtacgacg
     8221 cccaggcccc cagcacacgc cgactgacga agcggtgctc gcaccccgtg atgggatcgg
     8281 tgaactccag cacccgcgcc agcagttgga gcggatgtcg gaagtcgccg gaggccacgg
     8341 ggtcggtcac caccggatag aggggatcgc cgaggatcgg catccccaac gcgctcatat
     8401 ggacgcgaag ctggtgcgtc tgcccggtat gcggtacgag ccggtaccgg gcacgcccct
     8461 ctaggtgctc cagcaactcg acccggctga cggcgttggg ctcgccctcg acctcgcggg
     8521 ctgccatcac cccacgctcc ttgacgatcc ggctgcgcat ggtgcgcggc aggtccagtg
     8581 cggggtcgta cagggcgacg gcctcgtatt ccttcgtcac ccgcttgtcg cggaagagtg
     8641 actggtaggc gccgcgttcc tccggccgca cggtgaacag caccaggccc gccgtcagcc
     8701 ggtccagacg gtgggcggcg cccagggtcg ggacgtcgag ctgcctccgc agccgtgcca
     8761 gggccgtctc ggctacatga ctaccgcgcg gggtggtggc cagaaagtgc ggcttgtcgg
     8821 ccaccaccac gtgttcgtcc tggtagacga cgtcgaccgt gaacggtacc cgctcttcat
     8881 caggcaactt ccgctggaac cacaggtaca tcccgggggt gtacgccatg tcccgcggca
     8941 ccggacgtcc ggccacgtcc acgatctccc ccgcgtcgag cattgcgtcg atcacctcgg
     9001 ccccggcagc gagccgcgcc acgagatgat cccgcacggt cgcccatgct tccccggccg
     9061 gcaaccgcac tcgtaccgga tccacccctt cgcgctgcgg caggggagag ggcgggggcg
     9121 gactacggcg tctcatcacg atcaagccta cgccggggcg ctgacagcga aagggcgagg
     9181 cggcgctccg gaagggactt gcgcgctcct tgtacaccca gcgtcgggat tccgcggatg
     9241 tccccggttg tacgtgaggt gatccaggag ccagcccccg catcagccgc cgcaggagtg
     9301 ctgcgatctc cttcaggggc gcacgtcctg gtcgtgtacg catcagtgtg cggaaggggg
     9361 cgccttcccc cgtgggctta tggctggcac agttatagaa ctcgtccgga tcgtacaagc
     9421 ggtctaggag cggtatgagc ctcctgtagg ccgggtgcgt ggggtgatac ctgtgggcga
     9481 gtacaactgc gggacagtcg ttgtcggcgt tgcgcgttcc agcgacgttg caggcacttc
     9541 ccgccgacag gcatggccaa ctggcggccg aattcagcga gtttcctggc gaaggtccga
     9601 gccgtgtggg ccggtttcga cggttcacct tgctgcggca tgtctacggc ccgtccgtgc
     9661 gctccccctc gcaggcggaa ccgagcaagc tgctcggtcc tgtcgcggtg cgcagaccgt
     9721 gtccggccca tgccgactgg cgaacgcgaa caccactacc cggaggacgt catgagcgca
     9781 cccggcaagt gtgtccagct cgcagcacca acgggcccga ggcttctgcg agtaggcgct
     9841 tgaccgtgcg ggtcccctga ccaccaacgc cgcgagatcg tgcacaacac gtaggtcgtg
     9901 cggggggtca agaaggaggg tgcgatgttc cgccatgtac caggccgctc atgcaccgac
     9961 gctcccgcat ccgagcatcc ccgtgcctca ggaggaagtc atgtccccat tccgcatacc
    10021 cgaattcgac atgcccatcg cgtccagtgg tctctcaccc gcgatggacg cggtcgagcc
    10081 cgacatgtgg gcatgggccg ccgcccatca gcttgtgcgc ggtgaagcaa tgcgtcggca
    10141 tctggcccgt actcgaccgc agtgggccac cgcgatctac ttgcccggcg cggacgaagc
    10201 ccatctgcgg gtaccgaacc ggttcatgat ctgggccttc atcctcgacg acaacctcga
    10261 cgacagcatc agtgagggac acatcgagtc cgtcacgtac ttgctggacg aactcgtcgc
    10321 cgtctgccgc aacctgaaca cccccgtcac caacgaagga cgggctctca aagagatcct
    10381 cgacgacctc tcccaaggtc gcacaagggc ctggcgcaac gttctggggg aatccgtggt
    10441 gcgctggctg aacaccttcc tgccagaagc ccgcgcgagc cgggcgggcc gcgtcatgac
    10501 catcgacgaa tacctgcccc accgccgcaa cggagtctgc gagttcattt tcgcccacct
    10561 gcaggagtac gtcagggacg tcgaactgcc caagggggtg cgcgacctgc ccgcgatgga
    10621 acaggcccgc aaccttgcat gtgaatggac cggtctctac aacgacatct actccgtgga
    10681 gaaggaggag tcggtcggct atctccacaa cgccgttctt gtcgcgcgca ctcaccgcgg
    10741 ctgttccacc caggaggccg tggacatcgt cgctgccatt accaatagcc tcctccgcca
    10801 attcctcgcc gcctgcgatg cggcccccgc ccagatccgc gccgccgccg actacacccc
    10861 caccgtgctc gcccacgcca gcgatgtgat cgacggttac cgggaccttg tccgcgcgaa
    10921 ctacgactac caccgaggcg ccgcccgcta cagcgacgtc gccaagtaca tgccggaagc
    10981 catcaccacc agcggactgc ggcctgactg gagcttcacg ccgaccatca cccattcctg
    11041 agtcggcccc tgcaccggtg tgtgtcaact tagcggctga tcttggtgag tgtggtccga
    11101 ataggttgtc tcgtcccgct gatgtcatgt gatgtcgacg ggccttgagc gcgcccggtg
    11161 atccgaggcc gggccggcgg tcccactgac agaggggagc gtcggtccgg ttcctccgac
    11221 catggtccat cgcggatgca tgactggagt tacgccctca cccggggcga ggcatgagaa
    11281 agaggcggca cccatgaggt gccgcccctt cccgagccac agggactacg ccgccggcgc
    11341 cgccccctcc tgctccgcct cgatccgtgc gttccagtcc cgcttggagg cctgccagcc
    11401 gtcctcgtcg tggccgaggc gccagtagcc ggagatggac aggtcctctc gagccacgcc
    11461 gcgctcgacg cgcagcagat gacgtagttc cttcacgaag tgcgcctcgc cgtgcacgaa
    11521 ggcgtgcacc cggccctcgg ggaactccag agtctgaacc gcctcgagca gtgcctcgcc
    11581 gacccggcgg gaaccgcggt gcagccagat cacctccaca tcggagtcga tcttctgctc
    11641 ttcctcgggg cccgcgatct ccacgaaggc gtaggccgtg gcgccgtcgg gcagtgactc
    11701 cagggagcgg gcgatggcgg gcagggcgct ctcgtcgccg accagcagat gccagtcggc
    11761 gtccgggtcg ggggcgtagg cgccgccggg gcccatgaag cgcacggtct cgccgggctg
    11821 ggcgcgcatc gcccacggtc cggccaggcc ctcgtcgccg tgcagcacga agtccagtgt
    11881 cagctcacgg tgttcgggat cccaggcccg cacggtgtaa gtccgcgtca ccggccactg
    11941 ctcacgcggc agttcctcgc ggatgcgctc caggtcgaac ggctcggggt agctcacccc
    12001 ggcgggcgag aacagcatct tcacgtaatg gtccgtgcag gtgtccgccg cgaactcggc
    12061 gagcccgtca ccgcccagca ccacacgctg catgtgcggg gtcagccgtt cggtacggac
    12121 gacctgcgcg gagtggggct tccgcggctt gcgggcaggg cgtagcgcca tcactggcct
    12181 ccctcgttcc aagcttaggt ttacctaagt tagcacctcc gtccggcgaa tgccgctatg
    12241 aacgttcatg agaaaggggc atgtcgtgcg tacacggaac gaattccccg cgtcacaagc
    12301 cccatgcgcg cgatcgtgac gcccctcacg cccccggcga tctcacgccc ccagcgtcac
    12361 cagcagccgc tgaagcgacc cgcccaaccc ccagcgcgcc gccagctcgt ccagccccgc
    12421 cgcgtcccgg ggagcgcgcg gcagtgtggt gtcgacgtcg gacagcggga cgtccagcgc
    12481 gacccggacg accttcggcg cgacggcgac atacgggcgg gactcgtcca gccgcttgcg
    12541 ctgcgagggc gtcagcttcg ccttcgggtc gtcgaccgcc gccatgatcc cggccaggtc
    12601 gccgaactcc gccagcagct tcgccgccgt cttctccccg atccccggca cgcccggcag
    12661 gccgtcgctc gggtcgccgc gcagcagcgc cagatccgcg tacccgccgc cgtcgacgcc
    12721 gtacttctca cgcagccacg cctcgtcggt gagctggagg gtgccgacgc ccttgagggg
    12781 gtacagcacc cgcaccccgc gctcgtcgtc caccagctgg tacaggtcgc ggtcgccggt
    12841 gacgatgtcg accgggccct tcgcgcgcgc cgtgaacgtg ccgatcacgt cgtccgcctc
    12901 gtaccccgcg acgcccacgc gcgcgatgcc gagcgcgtcc aggacggcct cgatgaccgg
    12961 tacctgcggc gacagcgtgt ccggcacctc ctcctcgtcc ggtcccacct cgtgctcctc
    13021 ggcgacccgg tgcgccttgt aggaggggat cagctcgacc cgccactcgg gccgccagtc
    13081 ggcgtccatg caggccacca gcgcgtccgg gcggtggtcc ttgaccaggc ggtcgatgaa
    13141 gtcgaggagc ccgcgcacgg cgttcaccgg ggtgccgtcc ggggccttca cggagtccgg
    13201 gacgccgaaa taggcgcgga aatagagcga ggcggtgtcg aggagcatca gtcgtccggt
    13261 cacgccacgc atcatgccgt acggcactga cagtggcccc tcggtcagcg gcgcgttgcc
    13321 gtccacgacc accgctgtgg cctggaccac ttgtgcgttt gtgtgcgggg aaccagggca
    13381 ggcgcgcacc cggagcgacg ccccctgcgc aactgttgcg ccagtcgctc ccttcgtttg
    13441 ccgtcgagac aagaggtacc cgtgtcatcc aggcttgagg ccgaccatct gtacaaggtg
    13501 ttcggcagac gaccgaacga cgccgtcgag cggctccgca agggagccga ccgggaggag
    13561 ctgcgcgccg acggcaccac cgccgccgtg atcgacgcct ccttcaccgt ggaacccggc
    13621 cagatcttcg tcgtcatggg cctgtccgga tcgggcaagt ccaccctgct gcgcatgctc
    13681 aacgggctcc tggagccgac cgccggtcac gtccgcttcg acggccagga cctgaccgcg
    13741 atcggcgacc gcgagctgcg cgaggtccgc gcgaagaaga tcagcatggt cttccagcac
    13801 ttcgcgctct tcccgcaccg cagcgtcctg gagaacgccg cctacggtct cgccgtccag
    13861 ggagtgcccc gcgccgagcg cgagaagcgc gccgccgagg cactcgagct gtgcggtctg
    13921 ggcggctggg agaagtcctg gcccgacgag ctgtccggtg gcatgcagca gcgcgtgggc
    13981 ctcgcccgcg ccctcgccac cgacgccgac ctgctcctga tggacgagtc cttcagcgcg
    14041 ctcgacccgc tgatccgccg tgacatgcag gaccagctgc tccagctcca gaagaccctg
    14101 aagaagacca tcgtcttcat cacccatgac ctcaacgagg ccatgcggct cggcgaccgc
    14161 atcgccgtca tgcgcgacgg ccgtatcgtc cagaccggca ccgccgagga catcctcctg
    14221 cgccccgccg acgactacgt cgcctccttc atccaggacg tcgaccgctc ccgcgtactg
    14281 accgcgggct ccctcatgga cacctcggtc accgccgacg cccccctgtg cacctgcgag
    14341 accgcgacca ccgagacgac cttcgtggaa ctgtgcgcga tcagcgcccg actgtcgcac
    14401 cgcgtcaccg tcgtggacga cgacaacaag gtcatcggag tcgtgccgag gcagcggctc
    14461 gtcggtttcc tcggcgacga gagcgccgat ccctcgccct gcgacaaccc cgaggggaag
    14521 gtgaccgccc gtgcctaggc tgcgtctcgg tgactgggtc gactccggtg tggactggct
    14581 cgtcgcccac atgggctggc tcttcgacgc gatcaaggcg gtcgtcgagg gcatgtacga
    14641 cggcgtcaac gccgtcctga cggcacccga gcccctgctc ctcggcggca tcctcgccgt
    14701 gctcgcctgg tggctgcgcg gcctggtccc gggcgttctc gccttcggcg gcttcgcgct
    14761 gatcgactcc ctcgacctgt gggaccgcgc catgtcgacg ctggccctcg tcctggtcgc
    14821 cacggtcatc gccctggtga tctcggtccc gctgggcatc tgggcggccc gctccaagac
    14881 ggtcagcgcg gccgtacggc ccgtcctgga cctgcttcag acgatgccgt cgatggtgct
    14941 gctcatcccg gccatcctct tcttcggcct gggcaccgcc gccggcgtca tcgccaccct
    15001 gatcttcgcg ctcgccccgg gtgtccgtat gaccgagctg ggcatccgcc aggtcgacgc
    15061 ggagctggtc gaggccgccg aggcgttcgg caccacacca cgcgacaccc tgctgcgcgt
    15121 ccagctgccg ctcgcgctgc ccacgatcat ggccggcatc aaccaggtga tcatgctgag
    15181 cctgtccatg gtcgtcatcg ctggcatggt cggcaccggc ggcctcggcg gcgcggtcaa
    15241 cgaggccatc ggccagctcg acatcggctt cggcttcgag gcgggcgtcg gcatcgtcgt
    15301 cctcgccatc tacctggacc gcatcaccgg cgcgctcggc gcgcagatct ccccgctcgg
    15361 ccgccgtgcc gccgccaagg cccgtgccgc cggcggcgtc aaggtctgga gccaccggcc
    15421 ccgcccggtc gtcgccgtcg ccggcgtggc cgccctggcc ctcgtggcgg gcggcatggg
    15481 cgtcttcggc cccacctccg gtaccgccgc ggcctccgcc acggacgtcg gcaagggcaa
    15541 ggagatcaag ctcggctaca tcccgtggga cgagggcatc gcctccacct tcctctggaa
    15601 ggaactgctg gagcagcgcg gcttcaaggt caccaccagc cagtacgccg cgggccccct
    15661 ctatacgggc ctcgccaccg gccagatcga cttcgagacg gactcctggc tgcccaccac
    15721 acacgccgag tactgggaga agtacggcaa gcagctggag gacctcggct cctggtacgg
    15781 cccgacctcc ctggagctct ccgtcccctc gtacgtcaag ggcgtggact ccctggagga
    15841 cctcaagagc aacgcctcca agttcaaggg caagatcgtc ggcatcgagc cgagcgccgg
    15901 gatgatgggc atgctcaagg acaaggtcct caaggagtac ggcctggagg gctcctacga
    15961 cgtcgtcgac ggctcgacgc ccgcgatgct cgccgagctc aagcgcgcgt acgccaagaa
    16021 ggagcccatc gtcgtcacgc tctggtcgcc gcactgggcg tacagcgact acgacctgaa
    16081 gaagctcaag gaccccaagg gcgcctgggg caagggcgac ggcgtgcaca ccctcgcccg
    16141 caagggcttc gccggcgaca accccgaggt cggccagtgg ctgaagaact tctccatgac
    16201 cgagaagcag ctcaccggcc tggagtcgca gatcaccaag gccggcaagg gcaaggagca
    16261 ggacgccgtc cgcacctggc tgaagcagaa ccccggtctg ctggacaagt gggccccgat
    16321 cgacgcgtcg gacctcaaga accaggcggc cgggtgacct gacggacccg gaccgccgcc
    16381 cgaggggcgg gtcacgccgt acgtttttcc cctccgtacg gcatggcccg cccctcgcgg
    16441 catgtccgca tagtcacccg gcatgcctac gaaaaggatc cggccaacca cgcagcgcta
    16501 tcccaccgcc cgagccgcct catgcgaact ccccccgacc ggcaccgact tcccgctccc
    16561 gccaccactc tgaccaggcc cgcccgcagg agcgaagccg ggaacaccgg ggagcgcccg
    16621 agcattgaac agaacagcgg ctctcgcgta ctgagcggca gtcaggggcg ccatgggggc
    16681 gccctgcggg tgcggggtgc cgatccgctg gcgcgaaccc gtgggtcgca cttcccccaa
    16741 ggtgtcgtcg atgtgacggg cgcatgaaac ggtttgccga acatgcgtag ggtgcagaga
    16801 actcaacaga aggtgtgcgc cggacgacgg gcgcgaggac agtggaccga cgagcgaagg
    16861 agggagccgg agcgatgggc gaccacaaag aacagcccct tcgggtgggt gcggccgtgc
    16921 ggcggcgacg ccgtgcgctg gacctcaccc tcgccgtcgt ggccgagcgc agcggcctgt
    16981 cggtgccctt cctgagccag gtcgagaacg accgggcgcg ccccagccga agctccctgg
    17041 agaaggtcgc cgacgccttg cgcacgaccg ccgtggagct tctcgccgcc gccgacccgg
    17101 catgcagcgt cgacgtggtg cgcgccgaag ggaacgagtt gggacccgaa ccgcgggtgc
    17161 gttcgctggt gcgcggtcat caccagatgc acgcctcgga gttcaccggc gaccatgacg
    17221 ccggccgtga attccagtac cgcaacgacc agttgatgta cgtcgccgac ggcgccgtgg
    17281 agatcgaggc ggagggccgc gcctatcgcc tgggccgcgg ggactccctg tacctcaccg
    17341 gcggggtgcg gcaccgctgg cgggcgaccg tgccggacac gcgcgtggtc gtcgtcgcgg
    17401 tcgcggagca catcgaggcg gtccggaacc ggcctgggcg ctgatgcgcg tcgtctccct
    17461 ggtgccgtcg ttgaccgagg ccgtcgcgga cctcggtgtg ctggtcggcg cgacggactg
    17521 gtgcagccac ccggccgacc tggacgtcac gcgcgtgggc ggcaccaaga acccgaaggt
    17581 ggaccgcgtc ctcgccctcg cccccgacct ggtgatcgcc aacgaggagg agaaccgcga
    17641 gcccgacctc gccgccctgc gcgcggccgg cgtcgacgtc ctggtgacgg aggtgcggga
    17701 cgtgccgcag gccttcggtg aactcgcgcg tgtcctggag gcctgcgggg tgaccgcacg
    17761 cccgcgctgg ctggacgagg cggaggacgc ctggtcgtcg ccggccccgg accgccgtac
    17821 gacagccgtc gtgccgatct ggcgccgccc gtggatggtg ctgggccgcg acaccttcgc
    17881 gggcgacgtg ctggcccgcc tgggcgtgga ccacgtctac accacgcacg aggaccgcta
    17941 cccccgtata cccctggacg acctgcggga ggcggccccc gacctcgtcg tcctccccga
    18001 cgagccgtac cgcttcaccg ccgacgacgg cccggaagcc ttccccggac tgccctgcgc
    18061 gctggtcagc gggcggcacc tgacgtggta cggcccgtca cttgccgagg caccacgggt
    18121 actgacggcg gccctgcgag cagcgcaccg ctgaacagcc cccgggtggt gtgtacggcg
    18181 gcgacacccc aggccgcgac gaggacgacg tacagcccga cggcgagccc ttcgtagacg
    18241 acgagcccgg tgtgccgggc cagcgccgtg gcaccggtga cacaggtgcc gacgggaaag
    18301 gtgaacgccc accaggtcat cgcgaacccc atgccgtgcc ggcgggcccg caggacatgg
    18361 gcggtggcga gcccgaacca cagcagcgcg aaacccatga cggggacgcc gtacagcacg
    18421 gccagcatcc cgaagccctc gctgtacggc gccgggacca cgcccggcgc gacgtccgcg
    18481 aagacgccga ccgcggtggt ggactggccg agcggcccca gcacgagaaa cagcgacggg
    18541 gtgaggacga ggggcagcgg cccgcccgtg atcagccggg cgaacaccag gggcagcatc
    18601 agcagcgtcg cgagcagact cagcccgaac atcgcgacac aggccagcag cagcgtctcc
    18661 cgggcctgcc ccgccggcag atgcggcacg agcagcggcc ctagcgccgc ggacaccatg
    18721 ggcgcgacca gcggcagcag ccagacgggc gtggcctcgg acggctcgac gcgatggcgt
    18781 acggccatga ggtacgggac ggcgacggcg gccgccagcc cgatggccgt ccccgcggcg
    18841 aacagcacgg cgtccagagc gaccgccgcc gaggtcccga tccagtccct gccgacgacg
    18901 agggcaccgc cgccgacggc cagcagggcc atggacagac agccgtagaa gggcgccatg
    18961 gccgggtcga ggaggtgggc gcgggcctgg tcgcggtggt gcgaccagtg cgcggcgcgg
    19021 gcgccgagca gcgtgacgag cagggcgagg gacagggccc acacggcggc gcagacggtg
    19081 cgcagaccgg ggaggtggag cggcagtccg gcgccggcgg tggccacggc ggctgtgccc
    19141 atgacggcgg cgtaccagtt gggtccgagg tgacggacgc ccaggcggcg ggttggcccg
    19201 aggtgacggg ctgcggtgac catgtgtcca gcgtcgcttc gcggattccc tcccaccagg
    19261 gagtttgcct ctatggcgac ataaactgaa cttatgacag aggtggcgga ggggcagttt
    19321 cgcgcggggt ccttgtcaca ccgcgtcccg gagctcggcg cgctggaact gctgctcgcg
    19381 gtggcgcggc tcggcagtct cggcggggcg gcgcgtgagc tggggatcac ccagcccgcg
    19441 gcgagcagcc ggatccggtc gatggagcgg cagttggggg tggcgctggt cgatcgctca
    19501 ccacggggtt cgcggctgac ggacgccggg gcgctggtga cggactgggc gcggcggatc
    19561 gtggaggcgg cggaggcgtt cgacgcgggg gcgcaggcgc tgcgggaccg gcgggactcg
    19621 cggctgcggg tggcggcgag catgacgatc gcggagtatc tgctgccggg ttggctgctt
    19681 gccctgcggg cgcagcggcc ggatacggcg gtgtcgttgc tggccgggaa ctcggcggcg
    19741 gtggcggagc ggttgctggc cgacgaggcg gatctcgggt tcgtggaggg ggtgtcggtg
    19801 cctacggggc tggactccgt ggtcatcgcc cacgacaacc tgatcgtggt gacggcgccg
    19861 gggcatgtgt gggcgcgtcg gcggcgggcg ctgtcggggg cggagctggc ggccacgccg
    19921 ctgatcctgc gggaggaggg gtccgggact cggcaggtgc tggattcggc gctggggggt
    19981 ctggcgcggc cgctgatcga gctgtcctcg acgaccgcgg tcaaggccgc ggcggtgggt
    20041 ggggccgggc cggcggtgct gagtgagctg gcggtggggg aggagctgtc gatgcggcgg
    20101 ctggtgagcg tgccgctggc cgacgtgtcg ctggcgcggg atctgcgggc ggtgtggcct
    20161 acggggcatc ggccggtggg gcctgcgcgg gatctgttgg cgttgacgcg ggggtgaggc
    20221 tggtttttct cgcccccgcc gcccctaccc attcccgtcc cccgggggct gccgcccccg
    20281 gacccccgca tcggccctga acgggcctcg tcctcaaacg ccggacgggc tgaaaagcct
    20341 cacccgccgt cgcgagctga gaaacccggc ctcagccacc agccgcccgc accaaccccc
    20401 gcacaacccg caaatcctcc cccatctccg gatgccactg cacccccaac acccaccccg
    20461 ccgaaggcag ttccaccgcc tccacggtcc cgtccaccgc gtacgccgac gcgacgagcc
    20521 cctcacccaa ccggtccacg gcctgatggt gataggtcgg cacggacgac tcctccggga
    20581 cgagaccggc gtacagactc cccggcaccg gcttcacggt atggctgccg aacaccccga
    20641 cgacctccgc gtgcccgtcg atgtgctgaa cgagcgtccc cccgagggcg acgttcagca
    20701 gctgcatccc ccggcagatc cccagcaacg gcacccccgc gtcgagtgcc gcgtcgatga
    20761 gcgccagctc ccacgcgtcc cgctcccgcg ccgcaggccc cgtacgaggc tcccgctccg
    20821 cgccgtaccg gaccggctcg acgtcggggc cgccggcgat gaccagcccg tccagccggg
    20881 cgacggccgc cgcggcatgg gccgggtcgt ccggcggcag catcgcggcc aggccgcccg
    20941 cccgctgcac cagccgcgga tagccggccg gcagcagcgc cgcgtccagc tcccacacgc
    21001 cccagcgcgc accggactcc agataggtgc tgaccccgat c
//
