LOCUS       NZ_BNBM01000004        10216 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces lanatus strain JCM 4588 sequence04, whole genome
            shotgun sequence.
ACCESSION   NZ_BNBM01000004
VERSION     NZ_BNBM01000004.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces lanatus
  ORGANISM  Streptomyces lanatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 659677)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 61X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014655715.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 05:46:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,254
            CDSs (total)                      :: 9,172
            Genes (coding)                    :: 8,973
            CDSs (with protein)               :: 8,973
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 83 of 199
            Pseudo Genes (incomplete)         :: 153 of 199
            Pseudo Genes (internal stop)      :: 16 of 199
            Pseudo Genes (multiple problems)  :: 50 of 199
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:57:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 455990
            Orig. end                         :: 466206
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNBM01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            314..2464
                     /locus_tag="IE325_RS14010"
                     /old_locus_tag="GCM10018780_28300"
     CDS             314..2464
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117170.1"
                     /locus_tag="IE325_RS14010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_28300"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_373315162.1"
                     /transl_table=11
                     /translation="MVGVSDGQMSAQAPVAARTRVGRPLLSLALASMMEQVHAHSGGVY
                     LLAADEPVLEMAVMAGLPRAFAAPWERVGLSAPIPVADAVRDRRLVWVGGEEDMAARYP
                     RIAVVLPYPFALAALPVATETRAYGAIFVTWPGSHPPELSARDRDHLSAACRRLAVRLE
                     RAIEASRPPLPEPDLVAAPAFGGTAGLFGTVEAARMVQRLPYGLCSLDLHGRVGFANPA
                     AAELLGLPVSALLGNQLWAAVPWLNDPVYEDRYRAALFSQQTTSFVALRPPGEWLSFRM
                     YPSTTGLSVRISRARAVAEMARRGPQPGDAPSRLVTISQVLSLAGALTEAASVQDVVQL
                     VADEIVPAVGSQALLVLGSRAGRLHVLGHRGHADPHVVERFDGLPLAERTPGTHALSTG
                     VPAFFDSRQELERLYPSRYAAPDGFAAWAFLPLIASGRPVGTCVLAYSEPRSFPADERA
                     VLTSLGGLIAQALERAQLYDAKHQLAHGLQAALLPHTLPTLPGIEAAARYLPATQGMEI
                     GGDFYDLVPTEPGPTAAVIGDVQGHNVTAAGLMGQLRTAVRAYTTEGHSPEEVMRSTNR
                     LLIDLGSDLFASCLYLCLDPERGRAVMARAGHPPPLLRRPDGRVRVLDLAGGPLLGIDG
                     SPTYPTAEVDLTPGCVLVLYTDGLIESPGVDIEDALADLGRVLAEAGDRPLEELADLLV
                     RHGASVRERIDDVALLLLRARG"
     gene            complement(2582..3922)
                     /locus_tag="IE325_RS14015"
                     /old_locus_tag="GCM10018780_28310"
     CDS             complement(2582..3922)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007500817.1"
                     /locus_tag="IE325_RS14015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_28310"
                     /product="S1 family peptidase"
                     /protein_id="WP_190070165.1"
                     /transl_table=11
                     /translation="MSHKRIPKRKAAIAAGSVVALGAAAILLPNANASQDGAADDATTA
                     KTLKAADASDLASQLSGLLGDAFAGSYYDADSKQLVVNVIPGDNNNVVVQAKKAGAKVR
                     EVENSLSELASGAQTLKSEATIPGTSWAVDPRTNKILVTADSTVTGDNWDQLESTVDSL
                     GTGMATIKKSAGTFKTFLSGGDAIFAGGARCSAGFNVTAGDGSPAFLTAGHCGVAAEQW
                     SDAEGGQPIATVDQATFPGDGDFALVKYDDAATEAPSEVNLGDQTVAINQAADAEVGLQ
                     VFRMGSTTGLADGQVLGLDATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTQDGQAIGLT
                     SGGSGDCTVGGETFFQPVTTALAAVGATLGDAAGGGAGAGDDAGAGDDAGAGAGDDAGA
                     GDAGEEAGAGEEAGAGQEAGAGEEAGNGNADGTGNGLGEIVGNGVGN"
     misc_feature    complement(4021..4032)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            4112..4525
                     /locus_tag="IE325_RS14020"
                     /old_locus_tag="GCM10018780_28320"
     CDS             4112..4525
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676669.1"
                     /locus_tag="IE325_RS14020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_28320"
                     /product="PaaI family thioesterase"
                     /protein_id="WP_190070166.1"
                     /transl_table=11
                     /translation="MTMTTAEADKILSANFAPWVLALGLSVEAVDEHRAVLRMPWSEAL
                     AREGGALSGQALMAAADTATVIAVSAARGAYGPMTTVQQSTSFQRAVIGSDVLIEAVVT
                     KLGRRMAFADIAMTDESSSEIAARASTVYALLG"
     misc_feature    4825..4838
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.88"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="IE325_RS14025"
                     /old_locus_tag="GCM10018780_28330"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="IE325_RS14025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_28330"
                     /product="hypothetical protein"
                     /protein_id="WP_030052098.1"
                     /sec_met_domain="strepbact (E-value: 2.9e-35, bitscore:
                     115.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVATVEISDAELDNVSGGLQVNAVNGALDAVNGIAPVSGL
                     VDTVVGTVEGVTGLNTAPVSGLVAGL"
     gene            5357..6163
                     /locus_tag="IE325_RS14030"
                     /old_locus_tag="GCM10018780_28340"
     CDS             5357..6163
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="IE325_RS14030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_28340"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_190070167.1"
                     /transl_table=11
                     /translation="MQFRQQALAKLQSPEELDLPVRFARPQGWLVLSVTVVVMAAASVW
                     AVTGSVASTVSSPAILTHGQGSYILQSPVAGQVTAVLAEQGARLPANSPVLKVRTAEGE
                     KVVRTVAAGRVTGLAATIGQIISTGANVAAIEKVAHSEDPLYATVYVPAENAASIPDKA
                     AVDLTVSSVPTQEYGVLRGHVKSVDRSAQSAQQIAAFLGDTQLGEQFTKDGRPVAVLVK
                     LDKKSSTKSGYKWSSADGPPFDLTSMTLASGSIRLADERPVDWLLP"
     gene            6160..8382
                     /locus_tag="IE325_RS14035"
                     /old_locus_tag="GCM10018780_28350"
     CDS             6160..8382
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="IE325_RS14035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_28350"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_190070168.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.8e-39, bitscore:
                     129.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPAQETRSRRRAAPPKRPVPKGKSKTVRTPTVLQMEAVECGAAS
                     LAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYGLTAKGMQMDTAALAEVKT
                     PAVLFWEFNHYVVYDGMGRRFGRRGVYINDPAKGRRFVPMEDFDGSFTGVVLVMEPGED
                     FTKGGRKPGVLGAMPARLRGTAGTMPAAVLASLLLVVVGAAVPALSRTYIDMFLIGGQT
                     SLLGVLFASMGTCVALSLVLTWLQQANLLRGRIISSTLSSAKFLRHLLRLPVTFFSQRS
                     PADLVQRLQSNDAVAETLARDLAAAGVDAVVVILYALLLYTYDPQLTFVGIGVALLNVV
                     AMRVVIRLRATRTAKLRADSARLTNTAYTGLQLIETMKATGGEDGYFRKWAGQHATTLE
                     EQQRLGVPSAWLGVVAPTLATLNSALILWIGGMRAVEGGISVGLLVAFQALVTRFTAPI
                     TRLNGVAGRIQDFAADVARLKDVENFEADPLYGRPGTGESTRRLHGHVELQNITFGYSP
                     LDKPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYAPWEGVIRIDDQRIDDIPR
                     GALAASVSFVDQEVFLFEGTVRDNVALWDPSIPDDAVVDALRDAALYDVVMRRPGGIQS
                     KVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELVVMDNLRKRGCACV
                     VIAHRLSTVRDSDEIVVLEHGTIVERGRHEELVARGGAYAALVRER"
ORIGIN
        1 gcctcctcgg cacgcagtcc tcccgccctg cgtcctgcgg tgccctctct gaccacgtcc
       61 gccattacag cgcttatgag ccacctgcgc agcccctgtg aacgttccgc gcggccggag
      121 atgcgggcga tgctcgaaag gcgccgaaca tggcgtgacg catgggctgc gcacgccgcg
      181 gccacgggag tcgggacggc tgtcgcggga cttcgagggg ccgtacgtcc tcgccggtcc
      241 gctcgccgtg cgcggtgcgc tcggaggagg gagatttgtg ccctgggacc cgtcgcgcgg
      301 ctccgcgaag gaggtggtcg gcgtgtccga cggacagatg tccgcccagg cgccggtggc
      361 cgcgaggacg cgggtcggcc gaccgctgct gtcactggcg ctggcctcga tgatggagca
      421 ggtgcacgcg cactccggtg gcgtgtatct gctggcggcg gacgaacccg tgctggagat
      481 ggcggtcatg gcagggctgc ccagggcgtt cgcggcgccg tgggagcggg tcgggctgag
      541 cgcgccgatc ccggtcgcgg acgcggtgcg cgaccggcgg ttggtgtggg tgggcggcga
      601 ggaggacatg gcggcccggt atccgcggat cgcggtcgtg ctgccgtatc ccttcgcgct
      661 ggccgcgctg cccgtggcca ccgagacccg ggcgtacggc gcgatcttcg tgacctggcc
      721 cggttcgcat ccgcccgagc tgtcggcccg ggaccgggac catctgagcg ccgcctgccg
      781 gcggctcgcg gtgcggctgg agcgggccat cgaggcgagc cgcccgccgc tgccggagcc
      841 cgatctggtg gcggccccgg cgttcggcgg cacggccggt ctgttcggca cggtggaggc
      901 ggcgcgcatg gtgcagcggc tgccgtacgg gctgtgctcg ctcgatctgc acgggcgggt
      961 cggtttcgcc aacccggcgg cggccgagct gctcgggctc cccgtcagcg cactgctcgg
     1021 caaccagctg tgggcggcgg tgccctggct gaacgatccc gtctacgagg accgctaccg
     1081 ggccgcgctg ttcagtcagc agaccacgtc gttcgtggcg ctgcgcccgc ccggggagtg
     1141 gctgtcgttc cgtatgtatc cgagcacgac cgggctgagc gtgcggatca gccgggcacg
     1201 ggcggtggcg gagatggccc gccgtgggcc gcagcccggt gacgcgccgt cgcggctggt
     1261 gaccatctcg caggtgctga gcctggccgg tgcgctgacc gaggcggcga gtgtgcagga
     1321 cgtggtgcag ctggtcgccg acgagatcgt ccccgcggtc ggcagtcagg cactgctcgt
     1381 cctggggtcg cgggcgggcc ggctgcatgt gctgggccac cgcggccatg ccgacccgca
     1441 tgtcgtggag cggttcgacg gactgccgct ggcggagcgg acgccgggca cgcacgcgct
     1501 gagcaccggc gtgcccgcgt tcttcgactc ccggcaggag ctggagcggt tgtatccgtc
     1561 gcggtacgcg gcccccgacg ggttcgcggc gtgggcgttc ctgccgctca tcgcctccgg
     1621 ccgcccggtc ggcacctgtg tgctcgccta ctccgagccg cgttccttcc cggcggacga
     1681 acgcgcggtc ctgaccagtc tgggcgggct catcgcgcag gccctggagc gcgcccagct
     1741 ctacgacgcc aagcaccagt tggcgcacgg actgcaggcc gcgctgctcc cccacacgct
     1801 gccgacactg cccggtatcg aggcggcggc acgctatctg ccggccacgc agggcatgga
     1861 gatcggcggt gacttctacg acctggtgcc gacggagccg ggtccgacgg cggcggtgat
     1921 cggggacgta cagggccaca acgtgacggc ggccgggctg atgggccagc tccgcacggc
     1981 ggtacgggcg tacacgaccg aggggcactc tcccgaggag gtcatgcgca gcaccaaccg
     2041 gctgctgatc gacctcggtt ccgatctgtt cgccagctgt ctgtatctgt gcctcgatcc
     2101 ggagcggggg cgggcggtga tggcccgtgc cgggcatccg ccgccgctgc tgaggcggcc
     2161 ggacgggcgg gtgcgggtgc tcgatctcgc cggtggcccg ctgctgggca tcgacggttc
     2221 gcccacctat ccgacggcgg aggtggacct cacgccgggg tgtgtgctgg tgctgtacac
     2281 ggacgggctg atcgagtccc cgggggtgga catcgaggac gcgctcgcgg acctcggccg
     2341 ggtgctggcg gaggccgggg accgtccgct ggaggagctc gccgatctgc tggtgcggca
     2401 cggcgcgtcg gtgcgggagc ggatcgacga tgtcgcgctg ctgctgttgc gtgcacgggg
     2461 ctgaggggca cgcccgcaag acggagcggt ccggccctcc cgccggaggg ccggaccacg
     2521 tgtgtccgcg tcactcggga tgtgcccgga tcactgggat ccgtgtgccg gtgtcagggc
     2581 gtcagttgcc gacgccgttg ccgacgatct cgccgaggcc gttgccggtg ccgtcggcgt
     2641 tgccgttgcc ggcctcttca ccggcaccgg cctcctgacc ggctccggcc tcttcaccgg
     2701 cgcccgcttc ctcaccggcg tcgccggcac ccgcgtcgtc accggcaccc gcaccggcgt
     2761 cgtcgcccgc accggcgtcg tcaccggcgc ccgcgccgcc gcccgcggcg tcgccgaggg
     2821 tcgcgccgac cgccgccagg gcggtggtca ccggctggaa gaaggtctcg ccgccgaccg
     2881 tgcagtcacc gctgccgccg gaggtcaggc cgatggcctg cccgtcctgg gtgaacaggg
     2941 agccgccgct gtcgccgggc tcggcgcaga cgtcggtctg gatgagcccg gtgaccgtgc
     3001 cctccgggta gttcacggtg gcgtcgagcc cgagcacctg gccgtcggcg agcccggtgg
     3061 tgctacccat ccggaagacc tggaggccga cctcggcgtc cgcggcctgg ttgatggcga
     3121 cggtctggtc gccgaggttg acctcgctcg gggcctcggt cgccgcgtcg tcgtacttga
     3181 cgagggcgaa gtcaccgtca ccggggaacg tggcctggtc gacggtggcg atcggctgcc
     3241 cgccctcggc gtccgaccac tgctcggccg cgactccgca gtgcccggcg gtcaggaagg
     3301 cggggctgcc gtcgcccgcg gtgacgttga agcccgcgga gcagcgtgcg ccgccggcga
     3361 agatggcgtc gccgccggag aggaacgtct tgaaggtgcc ggccgacttc ttgatggtcg
     3421 ccatgcccgt accgaggctg tcgacggtcg actccagctg gtcccagttg tcgccggtga
     3481 cggtggagtc ggcggtgacg aggatcttgt tcgttctggg gtcgaccgcc caggacgtgc
     3541 cggggatggt cgcctcggac ttcagggtct gcgcgccgga cgccagctcg gacaggctgt
     3601 tctcgacctc gcggaccttc gcgccggcct tcttcgcctg gacgaccacg ttgttgttgt
     3661 cgccggggat gacattgacg acgagctgct tgctgtcggc gtcgtagtac gaaccggcga
     3721 aggcgtcgcc gagcagtccc gagagctgcg aggcgagatc cgacgcgtcc gccgccttca
     3781 gggtcttggc ggtcgtggcg tcgtccgccg cgccgtcctg tgaggcgttg gcgttgggca
     3841 gcaggatcgc ggccgctccg agcgccacca cgctgcccgc ggctatcgcg gccttgcgct
     3901 tcggaattcg cttgtgactc aaacttctcg acctcctggg gggacggggt ctgtgccgcc
     3961 gtctcagcgg cagctgttcg gggcgcctgg ttggcagttg gtacgcacgg gccgggtggg
     4021 gcgttcaatt ccgtttacgc gcaaccgact cggcacaccg aatcggccag ggacctcgtc
     4081 cggcgggccc gcgccgggaa caatccccca tatgacgatg acgaccgccg aagccgacaa
     4141 gatcctctcc gccaacttcg cgccctgggt cctcgccctg ggcctgtccg tcgaggcggt
     4201 ggacgagcat cgcgccgttc tgcgaatgcc ctggtcggaa gcgctggcac gggagggcgg
     4261 cgcactgtcg gggcaggcac tgatggccgc cgccgacacc gccaccgtga tcgccgtgtc
     4321 ggcggcgcgc ggggcgtacg ggccgatgac gacggtccag cagtcgacct cgttccagcg
     4381 cgcggtgatc ggctcggatg tcctgatcga ggcggtcgtg accaagctgg gccgccgtat
     4441 ggcgttcgcc gatatcgcga tgacggacga gagttcgtcg gagatcgccg cccgcgcgag
     4501 cacggtgtac gcactgctgg gctgacacgg tccgccaccg atcagtaacg gtcggtcgag
     4561 aacatgtacc ggaatccaag cttcaacgaa tccgcacatt caatactcaa cgaagtcacc
     4621 gggaccccgg gttctgcaca agcccctacc gccggttacg ccattgggcc gggagtgtcg
     4681 gattcgccac cactacgccc cgagcactcc cggacggatg caacagcccg tccgagccga
     4741 tgctgtgacg catgtgaaga agtcgccgac gagacgtgcg catgtctgca cggatggaga
     4801 ccgctgaggc caaagtgccc tggtgagacg gatgtttgat tggaagcccg cacccgcagc
     4861 gtgctttgat ccatcgcacc gcggggacca cgaacggttc ccggaaacgg gcgccggacg
     4921 cccgcggtcg cggccgtcat ctgtccccag agggggccgc tcccccttgt gaaataccca
     4981 ttaggaaggg aagttccatc atgaactcca ccccccaggt tgcgaccgtc gagatctccg
     5041 acgccgagct cgacaacgtc tccggcggcc tgcaggtcaa cgccgtgaac ggtgccctcg
     5101 acgctgtcaa cggcatcgcc ccggtctccg gcctggtcga cacggtcgtc ggcaccgtgg
     5161 agggtgtcac cggcctgaac accgccccgg tctccggcct ggtcgccggt ctctgatcgc
     5221 accttttgca accgctgagt cccggagcca ttccccggct ccggggctct tcgggtgccg
     5281 tgacacagac cggcaccgct ccgcagcacg accggccccc gcgcgccggt cctccgtcat
     5341 cctcaggtga ggttccgtgc agttccgcca acaggccctc gccaagctcc agtcaccgga
     5401 ggagctcgac cttccggtgc gcttcgcccg cccgcagggc tggctggtcc tctccgtgac
     5461 cgtggtcgtc atggccgccg cgtccgtatg ggcggtgacc ggatcggtcg cctccacggt
     5521 cagctcgccc gccatcctca cccacggcca gggcagctac atcctccaga gcccggtcgc
     5581 cggccaggtc accgcggtcc tcgccgagca gggcgcacgg ctcccggcca attccccggt
     5641 gctgaaggtc cgtaccgccg agggcgagaa ggtcgtacgc acggtcgccg cgggccgggt
     5701 caccgggctc gccgccacca tcgggcagat catctccacc ggcgcgaacg tcgccgccat
     5761 cgagaaggtc gcccactccg aggacccgct gtacgcgacg gtgtacgtcc ccgccgagaa
     5821 cgccgcgtcc atccccgaca aggcggccgt ggacctcacc gtctcctccg tgcccacaca
     5881 ggagtacggc gtgctgcgcg gccatgtgaa gtcggtggac cgctccgcgc agtcggcgca
     5941 gcagatcgcc gcgttcctcg gggacactca gctgggcgag cagttcacca aggacggccg
     6001 gccggtcgcc gtactggtga agctggacaa gaagtcgagc acgaagagcg gctacaagtg
     6061 gtcgtccgcg gacggaccgc ccttcgacct cacctccatg accctggcct ctggctcgat
     6121 ccgcctggcc gacgagcgtc ccgtcgattg gctgctgccg tgagccccgc acaggagacg
     6181 cggagcaggc gccgcgccgc cccgccgaag cgcccggtcc ccaaggggaa gtcgaagacg
     6241 gtccgcaccc ccaccgtcct gcagatggag gccgtggagt gcggcgccgc ctccctcgcg
     6301 atggtgctcg gccactacgg ccgccatgtc ccgctggagg aactgcgcat cgcgtgcggt
     6361 gtctcccgtg acggctcgcg cgccagcaat ctgctcaagg ccgcccgcag ctacggtctg
     6421 acggccaagg gcatgcagat ggacacggcc gccctcgccg aggtcaagac gccggccgtc
     6481 ctgttctggg agttcaacca ctacgtcgtc tacgacggca tgggccgccg cttcggccgc
     6541 cgcggcgtct acatcaacga ccccgccaag ggccgccgct tcgtgcctat ggaggacttc
     6601 gacggcagct tcaccggcgt cgtcctggtg atggagcccg gcgaggactt caccaagggc
     6661 ggccgcaagc cgggcgtgct cggcgcgatg ccggcccggc tgcgcggcac cgccggcacg
     6721 atgcccgccg ccgtcctggc aagcctgctc ctggtggtgg tcggcgcggc ggtgcccgcg
     6781 ctcagccgca cgtacatcga catgttcctc atcggcggcc agacctcgct gctgggcgtg
     6841 ctgttcgcgt cgatggggac ctgcgtcgcg ctgtcgctgg tgctgacctg gctgcagcag
     6901 gcgaacctgc tgcgcggccg catcatctcc tcgaccctgt cgagcgcgaa gttcctgcgc
     6961 catctgctgc ggctgccggt gacgttcttc tcccagcgca gcccggccga cctggtgcag
     7021 cgcctccagt cgaacgacgc cgtcgccgag accctggccc gcgacctcgc ggcggcgggc
     7081 gtggacgcgg tggtcgtcat cctctacgcg ctcctcctct acacctacga cccgcagctc
     7141 acgttcgtcg gcataggcgt cgcgctcctc aacgtggtcg ccatgcgcgt cgtcatccgc
     7201 ctgcgcgcca cccgcaccgc caagctccgc gcggacagcg cccgcctcac caacaccgcc
     7261 tacaccggtc tccagctgat cgagacgatg aaggcgaccg gcggcgagga cggctacttc
     7321 cgcaagtggg caggccagca cgccaccacg ctggaggaac agcagcgcct cggcgtcccg
     7381 agcgcctggc tcggcgtggt cgcccccacc ctcgccacgc tgaacagcgc cctcatcctc
     7441 tggataggcg gcatgcgcgc ggtcgagggc ggtatatccg tcggtctgct ggtcgccttc
     7501 caggccctgg tcacccgctt caccgccccg atcacccgcc tcaacggcgt cgcgggccgc
     7561 atccaggact tcgccgccga cgtggcccgt ctgaaggacg tcgagaactt cgaggcggac
     7621 ccgctctacg gccgccccgg caccggcgag tccacccgcc gcctccacgg ccacgtcgag
     7681 ctgcagaaca tcaccttcgg ctacagcccc ctcgacaagc ccctgctgac gggcttcgac
     7741 ctcaccgtgg gccccggcca gcaggtcgcc ctggtcggcg gctccggcag cggcaagtcc
     7801 accgtctccc ggctgatctc gggcctgtac gccccctggg agggcgtcat ccgcatcgac
     7861 gaccagcgca tcgacgacat cccgcgcggc gccctcgccg cctccgtctc cttcgtcgac
     7921 caggaggtct tcctcttcga gggcaccgtc cgcgacaacg tggcgctgtg ggacccgtcg
     7981 atccccgacg acgcggtggt ggacgcgctg cgcgacgccg cgctgtacga cgtggtgatg
     8041 cgccgccccg gcggcatcca gagcaaggtc gagcaggacg gccgcaactt ctccggcggg
     8101 cagcgccagc gcctggagat cgcacgggca ctggtgcgcc gccccagcat cctcgtcctc
     8161 gacgaggtga cgagcgcgct ggacgcggag accgaactgg tcgtgatgga caacctgcgc
     8221 aagcgaggct gcgcctgtgt ggtgatcgcg caccggctga gcaccgtgcg cgacagcgac
     8281 gagatcgtcg tactggagca cggcacgatc gtcgaacgcg ggcggcacga ggagctggtg
     8341 gcgcgcggcg gcgcgtacgc ggctctggtc agggagcgat gagatgacct ccgtacacgc
     8401 gggagaacac gagggagacc tcgtcctcgg cgcgctcggc cagatgggca cacgcatcga
     8461 ctgcgccggt ttcaaccgga tcgacctcga aggcccgcag gtgttgtggc tggtcgcgtc
     8521 cggcgcggtg gacctgttcg cggtcgacgc cggtcagcag ggccactggc atcacctcgg
     8581 ccgcctggag gcaggctcgc tgctgctcgg cccggtgccg ggaccgcagc acacgctggt
     8641 cgcccgcccg ctgcgtgact gcgtcgtcca ccgcatcgga ctgcgcgagc tgtaccagcc
     8701 cgcgaacacc cagacctggt cctacgacga gtacggcaac ccgcagtacg tgcccccgca
     8761 gaccagcccc ctggagtacg ccctcgccct gggcgtcggc cgcagcctca ccatcctctt
     8821 ccaggcgccg atggccaccg agcgggcggc cgaggtcacc gacgacgacg tcttctggat
     8881 gcaggtgccg cccggcagcg tgcagtacgg ctcgctgtac ggcgcggagg cggccgccga
     8941 tctgctgatg gacccggcgg tctggcagtc catggtcgac cagcagtacc gcctgctgac
     9001 cacgctggac cgctggatcg agcagctcga acgcacccat gagaaccgca ccgccgaggg
     9061 catcaaggcc ggcgaggcgg tgcgcgcgca ggccgatcag acgctgctgg cgtccatcgg
     9121 caagcgcggc ggcaagccga cgacggccgc cgacgcggac gcgagctacg ccgcctgcaa
     9181 gctggtcgcc gaggcggccg ggatcaccct gtccgagccc gcgcagagcg gcaccgagag
     9241 cgatcggctg gacccggtgg agcgggtggc catcgcgtcc cgggtgcgga cccggtccgt
     9301 acgccttgac gggcgctggt ggcgggacaa cgtggggccg ttggtcggtc accgggcgct
     9361 ctcgggcgct ccggtcgcgc tgctgtggcg gcggagcggc tatgtcgccg tgcatccggc
     9421 gaccggccgg gagacgccga tcgagaaggc gaacgcggag gagttcgagc ctcgcgcggt
     9481 gatgttctac cgcccgctgc ccgaccgtgc gttgagcccc ttgaggctgc tgcggttcag
     9541 catgcggggc acgggcggcg acgtcggcaa cctgatggtc agcggcctgg ttaccgtcgg
     9601 catcggtgcg ctggtgccga tcgcgaccgg caaggtgctc ggcgagttcg tgccgaaggc
     9661 gcagaccggg ctgatcgtgc agttctgtct ggccgtgatg atcagcggtg tcgtggcggc
     9721 ggcgttcatg ctgctgcaga acctgacgat cctgcggctt gagggccgta tcgaggcgac
     9781 gctgcaaccg gccctgtggg accggctgct gcggctgccc acgaagttct tcacccagcg
     9841 ctcgaccggt gagctggcca gccaggccat gggcatcagc gcgatacggc ggctgatggc
     9901 cggtgtcggc cccgtcgtcg cgtcgtcggt gacggtgggc gcgatgaacc tgacgctgct
     9961 gttctggtac agcgtgccta tggcaatggc ggcgatcggc atgctgatcg tcatcgccgc
    10021 ggtgttcctg gggctggggc tgtggcaggt gcgctggcag cggcggctgg tggtgctcaa
    10081 caacaagctg aacaaccagg cgttccagac gctgcgcgga ctgccgaaac tgcgggtcgc
    10141 ggcggccgag aactacgcgt acgcggcctg ggcctcgcag ttcgcgcaca gccgtgagct
    10201 ccagcagagg gtgggg
//
