LOCUS       NZ_BNBM01000001        11329 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces lanatus strain JCM 4588 sequence01, whole genome
            shotgun sequence.
ACCESSION   NZ_BNBM01000001
VERSION     NZ_BNBM01000001.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces lanatus
  ORGANISM  Streptomyces lanatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 1203604)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 61X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014655715.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 05:46:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,254
            CDSs (total)                      :: 9,172
            Genes (coding)                    :: 8,973
            CDSs (with protein)               :: 8,973
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 83 of 199
            Pseudo Genes (incomplete)         :: 153 of 199
            Pseudo Genes (internal stop)      :: 16 of 199
            Pseudo Genes (multiple problems)  :: 50 of 199
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:57:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1090677
            Orig. end                         :: 1102006
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNBM01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11329
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11329
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11329
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6329](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6329
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    1082..1095
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.58"
                     /tool="antismash"
     misc_feature    complement(1396..1409)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    1399..1414
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.03"
                     /tool="antismash"
     misc_feature    complement(1406..1419)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.08"
                     /tool="antismash"
     gene            1429..2289
                     /locus_tag="IE325_RS04820"
                     /old_locus_tag="GCM10018780_09750"
     CDS             1429..2289
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030618.1"
                     /locus_tag="IE325_RS04820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09750"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_190068373.1"
                     /transl_table=11
                     /translation="MITLVRRVTALCALGAALSVLASCAGPEEMTRAPLPAPSATASVS
                     PSLRAPTLAPGPGGTAPVFEHGPRSGDKAVALTFDADMTADQGPRAAQGERFDNPGLIV
                     ALRELKVPATVFMTGRWAEEYPEQARSIGRDPLFEVANHSYSHHSFTDDCYGLPTIAEE
                     RMRADVERAYAVFRKVGVRDAMPYFRFPGGCYDRRALRALTPVGVTAVQWDVVSGDAFA
                     TDAGAVARGVLDEVRPGSVVVLHCTRSAAPTTERVVRGVVPELRREGFRFVKVSELIRA
                     SGTRR"
     gene            2308..2517
                     /locus_tag="IE325_RS04825"
                     /old_locus_tag="GCM10018780_09760"
     CDS             2308..2517
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503632.1"
                     /locus_tag="IE325_RS04825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09760"
                     /product="hypothetical protein"
                     /protein_id="WP_190068374.1"
                     /transl_table=11
                     /translation="MSEDYCTIADAVKPPWGSPHALKAVGEDGPPYAECVLCREPTEYP
                     ESYKGITLCPVCEWQEAQRTACSG"
     gene            complement(2509..3180)
                     /locus_tag="IE325_RS04830"
                     /old_locus_tag="GCM10018780_09770"
     CDS             complement(2509..3180)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="IE325_RS04830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09770"
                     /product="ABC transporter"
                     /protein_id="WP_190068375.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLVRSQRWLPPFLLYVVFLGIGVQGGQPILDSLGY
                     TAAALLPVTAWLVRICVTNEPPAARSCVSAAVGPGRAHLAALLVALGTAVALGVVATVV
                     VTLISNPASADLQTRVPRLPAGAAGLLAVLACALLGTAVGALTTWPLLRSTGRAIPAML
                     LAALLSVVVTGSPAQAAVSGLVTGSNSGRVPMPVLPLAAAALLTAAAIAVACALTSRRS
                     P"
     gene            complement(3177..4052)
                     /locus_tag="IE325_RS04835"
                     /old_locus_tag="GCM10018780_09780"
     CDS             complement(3177..4052)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997024.1"
                     /locus_tag="IE325_RS04835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09780"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_190068376.1"
                     /transl_table=11
                     /translation="MQRDLRLDNVGRRYGLRGPWVLRGIHLTVPPGTLIRIEGPNGTGK
                     STLLRLLARIDAPSEGRVSGAPRTAYVPERFPTALPFTALGYLTHLGTVHGLSRPAATR
                     AALEWLDRFGAAPYARTPMAQLSKGSSQKVAVVQALLAEPELLVLDEAWTGLDSDARAE
                     LERAVAERTAGGTAVVFVDHDPRRLAGVPDATYTVRDSGLDLRTEQPPEEAAGPRVTVV
                     AQGPSGGQLPGDAQRAVVAAEEPAPGTHRLTVPASHSDILLRALLTARPPWHVVSVRQE
                     PAGPDTESRR"
     misc_feature    complement(3987..3989)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4051..4806
                     /locus_tag="IE325_RS04840"
                     /old_locus_tag="GCM10018780_09790"
     CDS             4051..4806
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997025.1"
                     /locus_tag="IE325_RS04840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09790"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_373315086.1"
                     /transl_table=11
                     /translation="MGASSPTARVGRLDRVNSDPSIDSPFRSEPSTRDEAPQFVLPLVV
                     RIERSAPPARTDALETSARAVLTILSDERSLGDGEWAEAMRDWQDARIRKVVRRARGAE
                     WRRAEELPGITVTGKSAEVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPPVDADPSGPV
                     LWLNPELDMSAGKTMAQTGHAAQLAWWELSDEERAAWRDAGFPLAVRTAQPGPWRELTG
                     GGLPLVRDAGFTEIAPGSCTVVADHPALR"
     gene            5001..6329
                     /locus_tag="IE325_RS04845"
                     /old_locus_tag="GCM10018780_09800"
     CDS             5001..6329
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122140.1"
                     /locus_tag="IE325_RS04845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09800"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_190068378.1"
                     /sec_met_domain="DUF692 (E-value: 1.2e-98, bitscore: 325.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIADAVERMPGIDWVETVAENVCPGHLPESLVR
                     LRERGVTVIPHGVSLGLGGADRPDADRLAALAERVAALGAPLVTEHIAFVRAGGPLTAS
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALIAWPGEEMSEGQFLY
                     ELADRTGVKLLIDVANLYTNHVNRGEDPAKALAELPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPQPVLDILTDLASRVSPPGVLLERDENFPEPAELEGELGAIRRALEKGAAERRAS
                     ASGNRATSADRLPSPHESSAPDLAPARQRLALAQAALLSALVAGTPVPEGFDRVRVGVQ
                     ARALAGKRADVVAKVAPELPVILGGGYRRAFLAYANGQPMTDGYRRDALDFAGYLLAEG
                     RCEDVGTRAELREWWLERSGPKPRSRRPGVRLARATRRVLLRR"
     misc_feature    complement(6548..6564)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.02"
                     /tool="antismash"
     misc_feature    6594..6610
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.35"
                     /tool="antismash"
     gene            6613..7392
                     /locus_tag="IE325_RS04850"
                     /old_locus_tag="GCM10018780_09810"
     CDS             6613..7392
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133764.1"
                     /locus_tag="IE325_RS04850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09810"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_190068379.1"
                     /transl_table=11
                     /translation="MRPRPRPPIKGRGIFSGTGLIITGLAATLLAMIFPIWSYADRSGT
                     GLDVLNAETVSTGYGPLSALDRDFLTKVRLAGLWELPAGEQARTKGTTPAVRTAGEHLI
                     EGHAFLDERVRDVAAKLSLALPNEATEQQRQWLATLDSAQGVEYDRQFANILRLAHGKV
                     FSVVAQVRASTRNSLVRKLADDANTTVLDHITVLEATGYVDFDALARDMVADSTPPLTA
                     PPAPPGPTADPAPAVPMTPPPSPTYTLPPAATSPRPS"
     misc_feature    complement(7412..7426)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.38"
                     /tool="antismash"
     gene            7496..8287
                     /locus_tag="IE325_RS04855"
                     /old_locus_tag="GCM10018780_09820"
     CDS             7496..8287
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073953.1"
                     /locus_tag="IE325_RS04855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09820"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_190068380.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAVAGTACTRLCLAAVQTAAMERKEATTARDHDLT
                     LYEAAFLSGGPRRVADLTLVSMARQQRLLLAHTGWATVVDPRGRDEMERSVIGAIGPEG
                     QSRIEPVRDAAAAAEAVGSLADRLVSAGLAVPDAARDGIGSAIRQVRLAAAVVLALGAV
                     ALLAPAQSDMPAHLVALWFALPFVLTLSCLAIARVEIHPYAQWASPAGQRLLSGLIRRS
                     GGGDDRTYLTFVAVRGVRAVGEPELRAAFAHRAHGASHWPD"
     gene            8429..10075
                     /locus_tag="IE325_RS04860"
                     /old_locus_tag="GCM10018780_09830"
     CDS             8429..10075
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (109-483). E-value:
                     1.5e-21. Score: 69.0. Matches aSDomain:
                     nrpspksdomains_IE325_RS04860_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981759.1"
                     /locus_tag="IE325_RS04860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09830"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_190068381.1"
                     /sec_met_domain="PF00561 (E-value: 7.5e-25, bitscore: 84.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYSAAGAMLLSALSLAPAGHAGAAPAADEQRGTAVAAARA
                     RAAGIDFDRCPKDQGLPGHVRCGTVSVPLDYARPDGKQIRLLVSRAPATHKDPHNSKHR
                     VPGKGPLVYNPGGPGASGLQFPLIGVLPAWKRLAAAYDLVGYAPRGVGPSARLSCQDPK
                     HYLKGPAQAPTYPSESYKKERVARAKAYARGCAERAGSALQYYNSLNNARDLDVLRAAL
                     GQSKLTFMGASYGTYFGALYAHLFPSHVRRMVFDSAVNPDPKQIWYHNNLAQSAAFEGR
                     WADFREWVAEHDDVYGLGITAAEVLDNYDEVRTRLASEPAGGKIGPGQLQEAFLQAGYY
                     DDYWPSRAHALSAYLKGDTKPLVELASPATEAAAEEENTNAVYTAVECNDAPWPTDWAV
                     WDRDNTRLARVAPFETWDNAWMNLPCAFWQAPRQQPLDVRTGPGELPPTLILAAERDAA
                     TPYDGALEMHRRLAGSVLVTERDAGTHGIAYGPNKCVNGYVDAYLLEGRLPTRHASCEG
                     HPEPKPQRAGERKTKKSAAQHKDRHETAARP"
     aSDomain        8756..9877
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE325_RS04860_Abhydrolase_1.1"
                     /evalue="1.50E-21"
                     /label="IE325_RS04860_Abhydrolase_1.1"
                     /locus_tag="IE325_RS04860"
                     /protein_end="483"
                     /protein_start="109"
                     /score="69.0"
                     /tool="antismash"
                     /translation="PLVYNPGGPGASGLQFPLIGVLPAWKRLAAAYDLVGYAPRGVGPS
                     ARLSCQDPKHYLKGPAQAPTYPSESYKKERVARAKAYARGCAERAGSALQYYNSLNNAR
                     DLDVLRAALGQSKLTFMGASYGTYFGALYAHLFPSHVRRMVFDSAVNPDPKQIWYHNNL
                     AQSAAFEGRWADFREWVAEHDDVYGLGITAAEVLDNYDEVRTRLASEPAGGKIGPGQLQ
                     EAFLQAGYYDDYWPSRAHALSAYLKGDTKPLVELASPATEAAAEEENTNAVYTAVECND
                     APWPTDWAVWDRDNTRLARVAPFETWDNAWMNLPCAFWQAPRQQPLDVRTGPGELPPTL
                     ILAAERDAATPYDGALEMHRRLAGSVLVTERDAG"
     gene            complement(10143..10874)
                     /gene="hemQ"
                     /locus_tag="IE325_RS04865"
                     /old_locus_tag="GCM10018780_09840"
     CDS             complement(10143..10874)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="IE325_RS04865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10018780_09840"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_190068382.1"
                     /transl_table=11
                     /translation="MSDDAPTTEPGRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADLMIWWHAETADQLQEAYNLFRRTK
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFYTGRRKSVAELVAGLA"
ORIGIN
        1 gctcaaggcg gtccgctccg acgcggacac ggtcggtctg gtgcgtgagc gtgtgatgcg
       61 ggaggcgcgg gcgctggccc ggctcagcca cccccatgtg gtgacggtcc atcagatcgt
      121 ggacgcggag ccgcacccgt ggatcgtgat ggagctggtc ccgggcatct cgctccaaca
      181 gcacctggac aacggcccgt tgacgccgcc cgcggcggcc cggctgggcc gtcaggtgct
      241 cgccgcgctg cgtgccgcgc acgcggcggg catccagcac cgggacgtca agcccgccaa
      301 catcctgctg cgccccgacg gcagcgccgt actcaccgac ttcggcatcg ccgccctcca
      361 gggcacgacg ggtctgacgg ccacgggcga gttcgtcggg tcgcccgagt acatggcccc
      421 ggagcggatc aggggccatg acgacggccc ggccgccgac ctctggtcgc tgggcgtggt
      481 cctgtacgtc tgcacggagg gcgtcagtcc cctgcgccgc ccgacgaccc tcgccaccct
      541 ggccgccgta ctcgacgacc cgctccccgc accgtcccgc tcgggcccgc tgaccccggt
      601 gctcgaagcc ctgctggtcc gggaccctgc ggcccgcccg gacgccgccc gcctggacat
      661 gatgctggcg caggtggagt cggggacgac accgcagtgg gccgcccagc cgacgatgac
      721 cgcgaccgcg ccgcagccga cgcccactca gctggacacg ccgcgcccca cgactccgga
      781 agcactgacc aggccccggc gccgtacacc cctggtggtg gccggcaccg cggtactggc
      841 gatcgcggcg gccacggcgc tggtcctagc cctccgcgca ggcgacacgg ccgcggacaa
      901 caaggccgac gcgacgccga aacccccggc caccacgacc gccggtccct cgaccccggg
      961 gaacgagctg gaggctcccc cgacacccct ccccaccggc cccgccccgg ccggccgctg
     1021 gatcgcccag ctccactccg aaccggtctc cgccggcatc gccaaccgcg accgcagact
     1081 cgccaccctc cgcgagacca ttccggggac caaggtcctg cgcagcggcg actacgcctc
     1141 cctgcgcgcc ggctactggg tcttctacgc ccccggcccc ttcgccaacg gccgcgcggc
     1201 cctcaccttc tgcgccaagc acgaccgcac cacggcgaac acctgcatgg gccgctatct
     1261 gagcaacgac gcgaccgagt tcctcctcca gtgccgccca ccggccgacg cgcctacggg
     1321 ccgctgcacc cgcaagggct gagcgatccg gcggccgggt gggtgacacc ggcccggcgc
     1381 acccgccgat acaccgaaat gaccgtttga tcatcatatc ttcatgcggt gatcactctc
     1441 gtacgacgcg tcaccgccct ctgtgccctg ggcgcggcgc tcagtgtgct cgcctcgtgt
     1501 gcggggcccg aggagatgac ccgggctccg ttgccggcgc ccagtgccac ggcctccgtc
     1561 tccccctcgc tcagggctcc gacgctcgcc ccggggcccg gcggcacggc cccggtcttc
     1621 gagcacggtc cccgcagcgg ggacaaggcc gtcgcgctga ccttcgacgc cgatatgacc
     1681 gccgatcagg ggccacgggc ggcgcagggc gaacggttcg acaatccggg gctcatcgtg
     1741 gcgctgcggg agttgaaggt gccggcgacc gtgttcatga cggggcgatg ggccgaggag
     1801 tatccggagc aggcccggtc catcgggcgg gatccgctct tcgaggtcgc caaccactcc
     1861 tacagccacc actccttcac cgacgactgc tacgggctgc cgaccatcgc cgaggaacgc
     1921 atgcgggcgg atgtggagcg ggcgtacgcg gtgttccgga aggtgggggt gcgggacgcg
     1981 atgccgtact tccggtttcc gggcgggtgc tacgaccggc gggcgctgcg ggcgctgacc
     2041 ccggtcgggg tcacggcggt gcagtgggac gtggtgagcg gggacgcgtt cgcgacggat
     2101 gcgggcgcgg tcgcgcgggg ggtgctggac gaggtgcggc ccgggtcggt ggtcgtgctg
     2161 cactgcacgc gcagtgccgc tccgacgacg gagcgggtgg tccgcggtgt ggtgccggag
     2221 ctgcgtcgtg aaggcttccg gttcgtgaag gtgtcggagc tgatccgggc ctcgggcacc
     2281 cggcggtgag cgtcctacgc tggaggtatg agcgaggact actgcacgat cgccgacgcg
     2341 gtgaagccgc cctgggggtc cccccacgcc cttaaggcag tgggggagga cgggccgccg
     2401 tatgccgagt gtgtgctgtg ccgggagccg accgagtacc cggagtccta caaggggatc
     2461 accctctgcc ccgtctgcga gtggcaggag gcacagcgca ccgcctgctc agggtgatct
     2521 gcgggaggtg agcgcgcagg ccacggcgat cgccgccgcc gtgagcaggg ccgccgcagc
     2581 caggggcagt acgggcatcg ggacccggcc cgagttggag ccggtgacca ggccgctcac
     2641 cgccgcctgc gccggggagc ccgtcaccac caccgacagc agggccgcga gcagcatcgc
     2701 cgggatcgca cggccggtgg agcgcagcag cggccaggtg gtgagcgcgc cgacggccgt
     2761 gccgagcagg gcgcaggcca gtacggcgag cagcccggcc gcgcccgccg gaaggcgcgg
     2821 tacgcgcgtc tggagatccg cgctcgcagg gttgctgatc agcgtcacga cgacggtggc
     2881 gaccacgccc agggccaccg cggtacccag cgccaccagc agcgcggcga ggtgggcgcg
     2941 cccggggccg accgccgcgg agacacaact gcgggcggcg ggcggctcgt tggtgacgca
     3001 gatccgcacc agccaggcgg tgacgggcag cagcgccgcg gccgtgtagc cgagcgagtc
     3061 gaggatgggc tggccgccct gcacgccgat cccgaggaac acgacgtaca ggaggaacgg
     3121 cggcagccag cgctgcgagc ggacgagcag ggcggcctgg tatctgagca gggcggtcat
     3181 cggcggctct cggtgtcggg gccggccggc tcctggcgca cgctcaccac gtgccagggc
     3241 ggccgcgcgg tcagcagggc gcgcagcagg atgtcggagt gggacgcggg cacggtgaga
     3301 cggtgggtgc cgggggccgg ttcctcggcc gcgacgacgg cgcgctgcgc gtcgccgggt
     3361 agctgcccgc cgctcggccc ctgggcgacg acggtcacgc gcggcccggc ggcctcctcg
     3421 ggcggttgtt cggtacggag gtcgagtccc gagtcccgca cggtgtacgt cgcgtcgggc
     3481 acccccgcga gccgacgcgg atcgtggtcc acgaagacga cggcggtgcc gcccgcggtg
     3541 cgttcggcga cggccctctc cagctccgcg cgggcgtcgg agtcgaggcc cgtccacgcc
     3601 tcgtccagga cgagcagctc cggttcggcg aggagggcct ggacgacggc gaccttctgg
     3661 ctgctgcctt tggacagctg ggccatgggg gtacgggcgt agggggcggc gccgaagcgg
     3721 tcgagccact ccagggcggc acgggtggcc gcgggccgcg acaggccgtg gacggtgccg
     3781 aggtgggtca gatagccgag ggcggtgaag gggagggcgg tggggaagcg ttcggggacg
     3841 taggcggtac ggggcgctcc gctgacgcgg ccctcgctgg gggcgtctat gcgggccagg
     3901 aggcgcagga gggtggactt gccggtgccg ttggggcctt ctatgcgaat gagggtgccg
     3961 gggggcacgg tgaggtggat gccgcgtaag acccatgggc cccgtaggcc gtagcggcgg
     4021 cccacgttgt ccagacgaag atcgcgttgc atgggggcat cctctcctac cgcccgggtt
     4081 ggcagactgg accgtgtgaa cagtgatccc tcgatcgaca gccccttcag gtccgagccc
     4141 agcacccggg acgaggcccc gcagttcgtc ctgcccctgg tcgtacgcat cgaacggtcc
     4201 gctcccccgg cccgcaccga cgccctggag acctccgccc gtgccgtcct gaccatcctc
     4261 agcgacgagc ggtccctcgg cgacggcgag tgggccgagg ccatgcgcga ctggcaggac
     4321 gcgcggatcc gcaaggtcgt gcggcgggcc cgcggggccg agtggcggcg ggcggaggag
     4381 ctgcccggga tcacggtgac cgggaagtcg gccgaggtgc gggtcttccc gccggtgccg
     4441 ctggacggct ggcccaagga actggccaag ctccaggtct ccggcaccga tctcgacgac
     4501 ccggaaccgc ccgtcgacgc cgatccctcc ggccccgtcc tctggcttaa ccccgagctc
     4561 gacatgtcgg ccggcaagac gatggcgcag accggacacg cggcgcagct cgcctggtgg
     4621 gaactgtccg acgaggagcg ggccgcctgg cgcgacgcgg ggttcccgct ggccgtacgc
     4681 accgcgcagc ccgggccctg gcgtgagctg acgggcggtg ggctgccgtt ggtgcgggac
     4741 gcggggttca cggagatcgc gcccgggtcg tgcacggtgg tcgcggatca tccggcgctg
     4801 cggtaggcgc ggccatgcgt cttcctcctg aacgctcccg ccgtcccgcg cgtatccccc
     4861 ggtacgggtc tgatgtccgg gcgggtccca acctcaaatg ttgctctgaa ggtggccccc
     4921 gaggggttcg ggccgagccc gcggggccat acccccgtca cgcggaacag gcgggggcgg
     4981 aagaggccga ggaggggacg atggagcgac tgggtacggg aatcggatgg cgtccggaga
     5041 tcgcggacgc ggtggagcgc atgccgggca tcgactgggt cgagaccgtc gccgagaacg
     5101 tctgtcccgg gcatcttccg gagtccctgg tacggctgcg tgagcgcggg gtgaccgtga
     5161 tcccgcacgg cgtctcgctg ggcctgggcg gcgcggaccg ccccgacgcc gaccggctgg
     5221 ccgcgctcgc cgagcgggtg gcggcactgg gggcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtacgggc gggcgggccg ctgacggcgt ccccgcgtct ggaggcgggg catctgctgc
     5341 cggtcccccg cacccgggac gccctggacg tgctgtgcga gaacgtccgt atcgcccagg
     5401 acgcgctgcc cgtgccgctc gccgtggaga acatcgccgc gctgatcgcc tggcctggcg
     5461 aggagatgag cgagggacag ttcctgtacg agctggccga ccgcaccggc gtgaaactcc
     5521 tcatcgacgt cgccaacctg tacaccaacc acgtcaaccg cggcgaggac ccggccaagg
     5581 cgctcgccga gctgccgctg gaggccatcg cgtacgtcca tgtcgcgggc ggcttcgaac
     5641 gcgacggcgt ctggcacgac agccacgccc accccgtccc ccagccggtc ctcgacatcc
     5701 tgacggacct cgcgtcccga gtgtccccgc cgggagtcct cctggagagg gacgagaact
     5761 tccccgaacc ggcagagctg gagggggagt tgggggcgat ccggagggcc ttggagaagg
     5821 gggcggccga gcggcgcgcg tcggcgtccg ggaaccgggc cacgtcagcg gaccggcttc
     5881 cgtcacccca cgagtcctcc gcacccgacc tcgcccccgc ccgtcagcgg ctcgccctcg
     5941 cgcaggccgc gttgctctcc gcgctcgtcg ccgggacgcc cgtgcccgag gggttcgacc
     6001 gggtgcgggt cggggtgcag gcgcgggcgc tcgcggggaa gcgggcggac gtcgtggcga
     6061 aggtcgcgcc ggagttgccg gtgatcctcg gtggcgggta tcggcgggcg ttcctcgcgt
     6121 acgcgaacgg gcagccgatg accgacggct atcggcgcga cgcgctggac ttcgcagggt
     6181 atctgctggc cgaggggcgt tgcgaagacg tagggacgcg cgcggagttg cgggagtggt
     6241 ggctggagcg gtcggggccg aagccgcggt cgcggcggcc gggggtgcgg ctggcccgtg
     6301 ccacgcgtcg ggtgctgctg cgacgctgag cgggtgtcgg atacgtcagc ttccgtccca
     6361 ccggtggacg cgggtggcgg cggtgagtgt gccggggtta acacctcggt ccgggacgcg
     6421 ggcgacctgc ccgcttcgcc ccataatgcc gacttgagcg gcccgtcgaa cctccccgtt
     6481 cgcccgtatc caccccgctt gcaaccattc cctcctgtac ggcggttggc gtaggcgccg
     6541 aagcgagccg aagtaatatg ccaaccccac ccccgaagcg cactaacgtg cggtgccgca
     6601 acaggaggca ccatgcgacc gcgaccgcga cccccgatca aaggccgagg catcttcagc
     6661 ggcaccgggc tcatcatcac cggcctggcg gcgacgctgc tggcgatgat cttcccgatc
     6721 tggtcgtacg ccgaccggtc cgggaccggt ctggacgtgc tgaacgcgga gaccgtctcg
     6781 accggctacg ggccgctgtc cgccctcgac cgcgacttcc tcaccaaggt ccggctggcc
     6841 gggctgtggg agctgcccgc cggtgagcag gcccggacga agggcacgac tccggccgta
     6901 cggaccgccg gggagcatct catcgagggg cacgcgttcc tggacgagcg ggtgcgcgac
     6961 gtcgccgcca agctcagcct cgcgctgccc aacgaggcca ccgaacagca gcggcagtgg
     7021 ctggcgaccc tggactcggc ccagggcgtg gagtacgacc ggcagttcgc caacatcctg
     7081 cgcctggcgc acggcaaggt gttctccgtc gtcgcccagg tccgggccag tacccgcaac
     7141 tccctggtgc gcaagctcgc cgacgacgcc aacaccaccg tcctcgacca catcacggtc
     7201 ctggaggcca ccggctacgt cgatttcgac gcgctggccc gggacatggt cgccgacagc
     7261 acgccgccgc tcaccgcgcc gcccgccccg ccgggtccga ccgccgaccc cgctccggcc
     7321 gtcccgatga ccccgccccc gtcgccgacc tacaccctgc ctccggccgc gaccagcccg
     7381 cgcccgtcct gacgccccgc tcggccggat ccggaacgtc acatcccggc aactctcggt
     7441 ccgcattgtg aacaaggcat ggtgttcgac ggagcatctg gcatagaaac acggcatgtt
     7501 ctgggtgctt cttctgctgc tggcctgggc cgtcgccggc acggcgtgca cgcggctgtg
     7561 cctggccgcc gtgcaaaccg cggccatgga aaggaaagag gcgaccacgg cccgggacca
     7621 cgatctgacg ctgtacgagg ccgcgttcct gtccggcggc ccccgccggg tcgccgatct
     7681 gaccctcgtc tccatggccc ggcagcagcg gctgctgctc gcgcacaccg gctgggcgac
     7741 ggtcgtcgat ccgcgcggcc gggacgagat ggagcgctcg gtgatagggg ccatcgggcc
     7801 ggagggccag tcccggatcg agccggtgcg ggacgccgcg gccgccgccg aggccgtggg
     7861 cagcctcgcc gaccggctgg tcagcgcggg gctcgcggtg cccgacgcgg cgcgtgacgg
     7921 catcggttcc gcgatccgcc aggtgcggct cgccgcggcg gtcgtcctgg cgctcggcgc
     7981 cgtcgcgctg ctggcgcccg cccagtcgga catgccggcc catctggtcg ccctctggtt
     8041 cgcgctgccc ttcgtcctca ccctgagctg tctggccatc gcccgcgtcg agatccaccc
     8101 gtacgcgcag tgggcctccc cggccgggca gcggctgctg agcggcctga tccggcgctc
     8161 cgggggcggg gacgaccgta cgtatctgac cttcgtcgcc gtacggggcg tgcgagcggt
     8221 cggcgaaccg gaactgcgtg cggccttcgc acatcgcgcg cacggggcgt cgcactggcc
     8281 cgactgacgc ggctccgggg cgggccgggt cgcggagttg gtgacgcata gggggcaatg
     8341 cggcaacttc ggcgaggcgg tgcttgcctt catcaacaga cgaacgaaat atcccttttg
     8401 ttgctgccgt gcaccgaagg gatacccgat gagagctgcc gccctctact cggccgcggg
     8461 ggccatgctc ctgtccgccc tctccctggc cccggccggc cacgccggtg ccgcaccggc
     8521 cgccgacgaa cagcgcggca cggcggtggc cgccgcgcgc gccagggccg ccggcatcga
     8581 cttcgaccgg tgccccaagg accaggggct gccgggccat gtgcggtgcg gcacggtgtc
     8641 cgtcccgctg gactacgcgc ggcccgacgg caagcagatc cggctcctgg tcagccgggc
     8701 gccggccacg cacaaggacc cgcacaacag caagcaccgg gtgcccggca agggcccgct
     8761 ggtctacaac ccgggcggac cgggcgcgtc cggccttcag ttcccgctga tcggggtcct
     8821 cccggcgtgg aagcggctgg ccgcggccta cgacctggtg ggctacgccc cgcgcggggt
     8881 ggggccgtcc gcccggctgt cctgccagga ccccaagcac tacctcaagg ggcccgcgca
     8941 ggcaccgacg tacccctcgg agtcgtacaa gaaggagcgc gtcgcccgcg ccaaggccta
     9001 cgcacgcggc tgcgccgagc gcgccgggag cgccctgcag tactacaact ccctcaacaa
     9061 cgcccgtgac ctggacgtcc tgcgggccgc gctgggccag tcgaagctga cgttcatggg
     9121 cgcgtcgtac ggcacctact tcggggcgct gtacgcgcac ctgttcccgt cgcatgtacg
     9181 gcggatggtg ttcgactcgg cggtgaaccc ggaccccaag cagatctggt accacaacaa
     9241 cctggcccag tcggccgcgt tcgaggggcg ctgggcggac ttccgggagt gggtcgccga
     9301 gcacgacgac gtgtacgggc tcggcatcac ggccgccgag gtgctggaca actacgacga
     9361 ggtgcgcacc cggctggcct cggagccggc gggcgggaag atcgggccgg ggcagctgca
     9421 ggaggcgttc ctgcaggccg gttactacga cgactactgg ccctcgcggg cgcacgcgct
     9481 gtcggcgtat ctgaagggcg acacgaagcc gctggtcgag ctggcctcgc cggcgacgga
     9541 ggcggcggcc gaggaggaga acacgaacgc ggtctacacg gccgtggagt gcaacgacgc
     9601 gccctggccg acggactggg cggtctggga ccgggacaac acccggctcg cgcgcgtggc
     9661 gccgttcgag acctgggaca acgcctggat gaatctgccg tgcgccttct ggcaggcgcc
     9721 caggcagcag ccgctggatg tgcggaccgg gccgggtgag ctgccgccga cgctgatcct
     9781 ggccgccgag cgggacgccg ccacccccta cgacggggcg ctggagatgc accggcggct
     9841 cgcgggctcg gtactggtga ccgagcggga cgccgggacg cacggcatcg cgtacgggcc
     9901 gaacaagtgc gtgaacggct acgtcgacgc gtatctgctg gagggccgtc tgccgacgcg
     9961 gcacgcgtcg tgcgaggggc accccgagcc gaagccgcag cgggcgggcg agcgcaagac
    10021 gaagaagtcg gccgcgcagc acaaggaccg gcacgagacc gcggcccgtc catagccgtc
    10081 cgggctcaca cggcgaagcc ccgcaccccg tcgaggggtg cggggcttcg tcgtacgtcg
    10141 gtctaggcga gcccggcgac cagctcggcc acggacttgc ggcggccggt gtagaacggc
    10201 acctcctcgc ggacgtgcat ccgggcctcg gaggcacgca ggtgacgcat gaggtcgacg
    10261 atgcggtaca gctcgtcggc ctcgaaggcg aggatccact cgtagtcgcc cagcgagaac
    10321 gaggcgaccg tgttggcgcg gacgtccggg tagccgcggg ccatcttgcc gtggtcggcg
    10381 agcatacggc ggcggtcctc gtcgggcagc aggtaccagt cgtaggagcg cacgaagggg
    10441 tagacgctga cgtagttgcg cggcgtctcg tcggcgagga acgccgggat gtgcgagcgg
    10501 ttgaactcgg cggggcggtg cagcgccatg ttcgaccaga cgggctcaag ggcccggccc
    10561 agcttggtgc ggcggaagag gttgtacgcc tcctggagct ggtcggcggt ctcggcgtgc
    10621 caccagatca tgaggtcggc gtcggcgcgc aggcctgaca cgtcgtaggt gccgcggatc
    10681 gtcacgtcct tggcggcgag ctggtcgaag agctcctgga cctcgtcggc gtagcccgcg
    10741 cggtcctcgg ggagcacgtc cttcagcttg aagacggacc agagggtgta gcggatgacc
    10801 tcgttgaggt ccttggccag cttgcccttg ttcgggatcc gaccgggctc ggtggtgggg
    10861 gcgtcgtcac tcatgtccct attctcctgc tcctccgtgg agactctgca ccgggttggc
    10921 ggtgaggtcc tgcacaccgc tcaggtctcc gtccagctgg tccacggcgg cgtacgcgct
    10981 cgcgatgcac gccgggatgc ccacgccgtc gtactgcgcg ccgcacacgg cgaggcccgg
    11041 caccttggcg atgtgctcgc ggacgcgggc cacgcgcgcg tggtggccga ccgggtactg
    11101 gggcagaccg tcggtccagc gggtgacgcg ggtccggacg ggggtggcgt cgaggccggt
    11161 ggcctgcttc aggtcgcggc gtgagacgtc gacgaggtgg gcgtcgtcgc ggcccaggat
    11221 gtccgtctcg ccgtaccgtc cgacggaggt gcgcaggacc agcgtgtccg ggtcctcgtc
    11281 ggcgatccag ccccacttct gggaggcgaa cgtcgatgcc ttgatggtg
//
