LOCUS       NZ_BNAU01000010        11416 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Amycolatopsis deserti strain CGMCC 4.7677 sequence10, whole genome
            shotgun sequence.
ACCESSION   NZ_BNAU01000010
VERSION     NZ_BNAU01000010.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Amycolatopsis deserti
  ORGANISM  Amycolatopsis deserti
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 251709)
  AUTHORS   Sun,Q. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 82X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014653945.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:43:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,561
            CDSs (total)                      :: 7,498
            Genes (coding)                    :: 7,407
            CDSs (with protein)               :: 7,407
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 32 of 91
            Pseudo Genes (incomplete)         :: 64 of 91
            Pseudo Genes (internal stop)      :: 8 of 91
            Pseudo Genes (multiple problems)  :: 12 of 91
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:49:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 127301
            Orig. end                         :: 138717
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNAU01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11416
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11416
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11416
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6416](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6416
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            41..988
                     /locus_tag="IE290_RS33020"
                     /old_locus_tag="GCM10017786_66950"
     CDS             41..988
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986926.1"
                     /locus_tag="IE290_RS33020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_66950"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_308431400.1"
                     /transl_table=11
                     /translation="MSTVEDVSTTPQTTARPGKAALSEGKRAERRLGWLLCAPAAIVMV
                     AVTGYPIVYSIWLSLQRYDLRFPQNTEFVGLANYAAVLSDSYWWTAFGTTMALTIISVA
                     IELVLGMLLALIMHRTLVGRGLVRTVSLIPYGIVTVVAAFSWYYAWTPDTGYLANTLAG
                     DSAPLTQYLPSMAIIILAEVWKTTPFMALLLMAGLALVPDDLLKAAAMDGANGWQRFIK
                     VIVPVMKPAILVALLFRTLDAFRIFDNIYVLTGGQNDTSSVSMQTYNNLIKGLNLGIGS
                     TMSVLIFITVAIIAFIFIKLFGTAAPGSDKGGKR"
     gene            988..1830
                     /locus_tag="IE290_RS33025"
                     /old_locus_tag="GCM10017786_66960"
     CDS             988..1830
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1069: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072935.1"
                     /locus_tag="IE290_RS33025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_66960"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_191248652.1"
                     /transl_table=11
                     /translation="MVMGGAVTTGRKTRWTLIDIVVVVYALVPVLWILSLSLKTKDTID
                     DGKFIPRSWTLQNYADIFQTLDFVRALINSIGISLIATLIAVILGTMAAYAIARLDFPG
                     KQLLVGVSLLIAMFPQVSLVSPLFNIERTLGLFDTWPGLILPYITFALPLAIYTLSAFF
                     REIPWELEKAAKMDGATPGQAFRRVIAPLAAPGVFTTAILVFIFCWNDFLFAISLTSTN
                     ASRTVPAALSFFTGASQFEDPTGTICAAAVVITVPIILFVLFFQRRIVAGLTSGAVKG"
     gene            1835..3016
                     /locus_tag="IE290_RS33030"
                     /old_locus_tag="GCM10017786_66970"
     CDS             1835..3016
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420930.1"
                     /locus_tag="IE290_RS33030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_66970"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_191248653.1"
                     /transl_table=11
                     /translation="MAEIVLDKVSKRYPDGALAVSEVDLQVADGEFIILVGPSGCGKST
                     TLNMVAGLEDISSGELRIDGERMNEKAPKDRDIAMVFQSYALYPHMSVRENMAFPLRLA
                     KMDDATVKAKVEEAAEILDLTQHLDRKPSNLSGGQRQRVAMGRAIVRSPKAFLMDEPLS
                     NLDAKLRGQMRTSVSKLQRQLGTTTLYVTHDQVEAMTLGDRVVVLRAGYVQQIGSPQFL
                     YDHPTNLFVAGFIGSPSMNFVPATLEDGSLKSALGAVQLPDRLRRLAEAAGAPREVIAG
                     IRPEHFEDAGLLDESARGKGCVFSAHVDVLESMGSEKYAYFTVEGATATSSELAELAAD
                     AGADDVPGGGASITARLSPSSGAREGGTLDVWFDVQKVQLFDPASGKNLTFEG"
     gene            3336..4271
                     /locus_tag="IE290_RS33035"
                     /old_locus_tag="GCM10017786_66980"
     CDS             3336..4271
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE290_RS33035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10017786_66980"
                     /product="hypothetical protein"
                     /protein_id="WP_191248654.1"
                     /transl_table=11
                     /translation="MADGQGSGGAMIALGVVCAVLGAVAAAFGAWLQHRGVTAVGRTHP
                     AALLRNPAWRLGVLVLTACTVLQALALALAPVTVVTPIVVLALPILTVLNGPRDPLTRV
                     AVAAVTLAVAVFVTLTARDAKPATIPPDTVLDAGQLVGIIVVSLVAVAAFAQGIARSAA
                     LAVAAGVGYGLVAVLIKDVLHADGLPWLSVLATVLAFATAAWLVQHAYASGPPDVVVGC
                     QTAANPVVATGLGMTVLGEAPALDLPTLAACGATAIAGIAVLAVRRDSLTGARATPSSN
                     SPGRPAAWSGTAATGPGPRRASTPRPTRPS"
     misc_feature    complement(3367..3382)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.68"
                     /tool="antismash"
     misc_feature    complement(4344..4358)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            <4462..>4653
                     /locus_tag="IE290_RS33040"
                     /pseudo=""
     CDS             4462..>4653
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031802.1"
                     /locus_tag="IE290_RS33040"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="MFS transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MADPHHARRWLILVMIGLAQLMVVLDATIVNIALPTAQHELGFSS
                     DARQWVVTAYALAFGSRLL"
     gene            5001..6416
                     /locus_tag="IE290_RS38350"
                     /old_locus_tag="GCM10017786_67000"
     CDS             5001..6416
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF013077.5"
                     /locus_tag="IE290_RS38350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_67000"
                     /product="nitroreductase family protein"
                     /protein_id="WP_191248655.1"
                     /sec_met_domain="PF00881 (E-value: 7.2e-25, bitscore: 81.9,
                     seeds: 78, tool: rule-based-clusters)"
                     /sec_met_domain="AfsA (E-value: 3.4e-09, bitscore: 30.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVPVDEVLTTTRAVRRRLLLDVPVEDQVIEDCLEVALQAPNGGN
                     RQPWRWIVVRDEGQRARIAEVYRRCFADYVAELGNPGPSRDLDSARFLADNLHRVPVLV
                     LPCLVAPRLPEGSQASPWGSLLPAAWSYMLAARARGLGTAWTTCHLRREAEIADILGLP
                     PGIRQGVLIPTAYHDGGPFRPARRTPLADVLVVDRWPRPERRTRELTADLDDPFFFDHE
                     LDHLPGMLLLSAALDLVRPAGGDPAVTLALDFRGMGELDEPVRLTAESPDDSGRLWQVH
                     ADQDGRTIMTGTIERIGAAASAPGTVAGTVKPVEQRLVHRRRAENVFVGDLVESEHDWR
                     VTVLPAPAGHHLDGGRTAERLIEAARQFVTLLEYADNQRQEGTRFVLTRLAATVPAGPS
                     AGAVLSWAKTGSRGNRPRYDVRILDAGGRAVGSVTVCGRALSDAAFRRLRGRPAPLATA
                     APVLSAGGPAAPR"
     gene            complement(6385..7233)
                     /locus_tag="IE290_RS33050"
                     /old_locus_tag="GCM10017786_67010"
     CDS             complement(6385..7233)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986934.1"
                     /locus_tag="IE290_RS33050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_67010"
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="WP_191248656.1"
                     /transl_table=11
                     /translation="MNPARDTGFPRADAEHDFLRARRRQVLSRLANWLRREPDDVNIML
                     PFDEVVDALGYVSERRVGLRVIPLNSIVGSVDRGRDFDRRFRPTSGRVRERWERLALAA
                     RRGEHIPPIEVYRVGDLHFVIDGHHRVSVAYALGLETIEASVTEVHTKLNPKGIRYRGD
                     LIVKDYRRLFLSRVPLSGEARAAIVVSDPWDYAHLGEHVEAWGFRLMQSEGKFLDRATI
                     ARRWYDEEYLPVVEMMRQAELIGDRTEAEAYMWVAGERYRLIRQHRWDDEVIEALRARR
                     R"
     gene            complement(7230..7898)
                     /locus_tag="IE290_RS33055"
                     /old_locus_tag="GCM10017786_67020"
     CDS             complement(7230..7898)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420923.1"
                     /locus_tag="IE290_RS33055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_67020"
                     /product="metallophosphoesterase"
                     /protein_id="WP_191248657.1"
                     /transl_table=11
                     /translation="MRALVVADEVDERLWAGIGPGCDVGLVLAAGDLPFDYLEYLTDAL
                     GGVPCVFVPGNHDPDLSGFTRHGGLFLRAGFPQAWPGPAGGINADGRVVDVCGLRIAGL
                     GGSVRYNDGPNQWTQAQQARRARRLVRQAARRRRRDGRGVDVLLTHAPPRGVGDREDPP
                     HRGFDCLHPTVERMRPDWLLHGHIHPYGEHPREHRMGETRVRNVVGKHLIEFTAHEVRT
                     "
     gene            complement(8069..9154)
                     /gene="corA"
                     /locus_tag="IE290_RS33060"
                     /old_locus_tag="GCM10017786_67030"
     CDS             complement(8069..9154)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /GO_process="GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="corA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643594.1"
                     /locus_tag="IE290_RS33060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_67030"
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="WP_191248658.1"
                     /transl_table=11
                     /translation="MPAIPSLGGLRGRGNGRAARVEPPVPVPVSAYIVDCAVYVDGERL
                     PGRWTHAEAIKEVRRRRDGFVWIGLHEPDQQQIQGIAEVYGLHELAVEDAVQAHQRPKL
                     ERYDDTLFMVLKTVRYVEHDSPTTANEIVETGELMAFLGLDFIITVRHGAHSGLARLRR
                     DLDEDPERLVNGPAAVLHAITDLIVDQYLDVTSSIEEDIDEMEARVFAPRSTVSAEQIY
                     LMKREVLELRRAVMPLVTPVRRLAEGFTRLVPDEVRSYFRDVDDHLTTVSERVGNFDEL
                     LTTLVNATVAKITLQQNSDMRKITSWAAMITVPTMIAGIYGMNFDFMPELHWQFGYPAV
                     LVMILGICLLLYRIFRKNNWL"
     misc_feature    complement(9313..9327)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.6"
                     /tool="antismash"
     gene            9472..9975
                     /locus_tag="IE290_RS33065"
                     /old_locus_tag="GCM10017786_67040"
     CDS             9472..9975
                     /GO_function="GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668029.1"
                     /locus_tag="IE290_RS33065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_67040"
                     /product="MaoC family dehydratase"
                     /protein_id="WP_191248659.1"
                     /transl_table=11
                     /translation="MQFGRYFEEFEVGAVYKHWPGKTVTEYDDHLFCLLTMNHHPLHMD
                     AHYAAETTDFGKNVVVGNYIYSLLLGMSVPDVSGKAIANLEIESLRHVKPTFHGDTIYG
                     ETEVLEKTPSKSKDDRGVVYVETRGYKQDGTIVCIFRRKVLVPKQNYGEARGGEQPGRP
                     VPHE"
     gene            9986..11089
                     /locus_tag="IE290_RS33070"
                     /old_locus_tag="GCM10017786_67050"
     CDS             9986..11089
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331477.1"
                     /locus_tag="IE290_RS33070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_67050"
                     /product="DUF2332 domain-containing protein"
                     /protein_id="WP_191248660.1"
                     /transl_table=11
                     /translation="MTIGLAEAKRQLTDFAGSAAGESPLYEHLASKAAGDDEVAGLLAA
                     APGGASPALLFAVAHRLIQADPIHPLSRYYPSLGGFDGVDGQTWPVFREFLLAHADDAA
                     GMLASKSLANNEVRRAASAFPGVAMAAKQAGGKIALIEVGTAGGLLLGMDKFAYRYQCD
                     GGEQLVAGPAKAAVGLHCALDLAPGAVLPKLPKKLAVAARVGLDPAPLDLSDEDELAWL
                     EACVWADQPDRVRLLRAAALAQRKSPPEIIAAGAAGGFGEAVSRVPADLPLVVLTSRTM
                     GHIPDAERAEFLSSMAALGESRPLWWVMDETYEHGLSAVLPGRDELAFARDGLTTLGLA
                     RRTADGWDALALARTTRYGARMTWLAA"
ORIGIN
        1 gcaagctgac cgacgcgctg aactcgaaag gggtgatccc gtgagcacgg tcgaggacgt
       61 ttccacgacg ccacagacca cggcgagacc gggcaaagcg gccctgagcg aagggaagag
      121 ggcggagcgg cggctgggct ggctgctgtg cgcgcccgcg gcgatcgtga tggtcgcggt
      181 caccgggtac ccgatcgtgt actcgatctg gctgtcgctg cagcgctacg acctgcggtt
      241 cccgcagaac accgagttcg tcgggctggc caactacgcg gcggtgctgt cggactccta
      301 ctggtggacg gcgttcggca cgacgatggc cctcacgatc atctcggtgg ccatcgagct
      361 ggtgctgggc atgctgctcg cgttgatcat gcaccgcacg ctcgtcggcc gcggcctggt
      421 gcgcacggtc tcgctgatcc cgtacggcat cgtgaccgtg gtcgccgcgt tctcctggta
      481 ctacgcgtgg accccggaca ccggctacct ggccaacacg ctggccggtg actcggcccc
      541 gctgacgcag tacctcccgt cgatggcgat catcatcctc gccgaggtgt ggaagacgac
      601 gccgttcatg gcgctgctgc tgatggccgg gctcgcgctg gtgccggacg acctgctcaa
      661 ggccgcggcg atggacggcg cgaacgggtg gcagcggttc atcaaggtca tcgtgccggt
      721 gatgaagccg gcgatcctgg tggcgctgct gttccgcacc ctggacgcgt tccgcatctt
      781 cgacaacatc tacgtgctca ccggcggcca gaacgacacg tcgtcggtgt ccatgcagac
      841 ctacaacaac ctgatcaagg gcctgaacct gggcatcggg tcgacgatgt cggtgctcat
      901 cttcatcacg gtcgcgatca tcgcgttcat cttcatcaag ctgttcggca ccgcggcgcc
      961 gggcagtgac aagggaggga agcgctgatg gtgatgggcg gagcggtcac caccggccgc
     1021 aagacgcggt ggaccctgat cgacatcgtg gtggtggtct acgcgctggt gccggtcctg
     1081 tggatcctgt cgttgtcgct gaagacgaag gacaccatcg acgacgggaa gttcatcccg
     1141 cgctcgtgga cgctgcagaa ctacgcggac atcttccaga ccctggactt cgtgcgggcg
     1201 ctgatcaact cgatcggcat ctcgctcatc gcgacgctga tcgcggtgat cctggggacg
     1261 atggcggcgt acgcgatcgc gcggctggac ttccccggca agcagctgct ggtcggggtc
     1321 tcgctgctga tcgcgatgtt cccgcaggtg tcgctggtga gcccgctgtt caacatcgag
     1381 cgcacgctcg ggctgttcga cacctggccg gggctgatcc tgccgtacat cacgttcgcg
     1441 ctgccgctgg cgatctacac gctgtcggcg ttcttccggg agatcccgtg ggaactggag
     1501 aaggcggcca agatggacgg cgcgacgccg gggcaggcgt tccggcgggt catcgcgccg
     1561 ctggcggcgc cgggtgtgtt caccaccgcg atcctggtgt tcatcttctg ctggaacgac
     1621 ttcctgttcg ccatctcgct gacctcgacc aacgcgtcgc ggacggtgcc ggcggcgttg
     1681 tcgttcttca ccggggcctc gcagttcgag gacccgaccg ggacgatctg cgccgccgcg
     1741 gtcgtgatca ccgtcccgat catcctgttc gtgttgttct tccagcgccg catcgtcgcg
     1801 gggctgacct ccggtgcggt gaaggggtag cgccatggct gagatcgtgc tggacaaggt
     1861 gagcaagcgc tatccggacg gtgcgctcgc cgtgtccgaa gtggacctgc aggtcgccga
     1921 cggcgagttc atcatcctgg tcggcccgtc cgggtgcggg aagtcgacca cgctgaacat
     1981 ggtggccggg ctggaggaca tctcctccgg tgagctgcgc atcgacggcg agcggatgaa
     2041 cgagaaggcg ccgaaggacc gggacatcgc gatggtgttc cagtcctacg cgctgtaccc
     2101 gcacatgagc gtgcgggaga acatggcctt cccgctgcgt ctggccaaaa tggacgacgc
     2161 cacggtgaag gcgaaggtcg aggaggccgc ggagatcctc gacctgaccc agcacctgga
     2221 ccgcaaaccg tccaacctgt ccggtgggca gcggcagcgg gtggcgatgg gccgggcgat
     2281 cgtgcgcagc cccaaggcgt tcctgatgga cgagccgctg tccaacctgg acgccaagct
     2341 gcgtgggcag atgcgcacgt cggtgtcgaa gctgcagcgc cagctcggca cgacgaccct
     2401 gtacgtcacg cacgaccagg tcgaggcgat gacgctgggc gaccgggtgg tggtgctgcg
     2461 ggccgggtac gtgcagcaga tcgggtcgcc gcagttcctc tacgaccacc cgaccaacct
     2521 gttcgtggcc gggttcatcg ggtcgccgtc gatgaacttc gtgccggcga cgctggagga
     2581 cgggagcctg aagagcgcgc tgggcgcggt ccagctgccg gaccggctgc gccgcctggc
     2641 ggaggcggcc ggtgcgccgc gcgaggtgat cgccggcatc cggccggagc acttcgagga
     2701 cgccgggctg ctggacgagt cggctcgcgg caagggctgc gtgttcagcg cgcacgtcga
     2761 cgtgctggag tcgatggggt cggagaagta cgcgtacttc acggtggaag gcgcgacggc
     2821 gacgtcgtcc gaactggcgg aactggcggc ggacgccgga gcggacgatg tgccgggcgg
     2881 cggagcgtcc atcacggccc ggctgtcgcc atcgtcgggc gcccgggagg gcggcacgct
     2941 cgacgtgtgg ttcgacgtgc agaaggtgca gctgttcgac ccggcgtcgg ggaagaacct
     3001 gaccttcgag ggctgaggcc gccttgtggg cccgcgccgg cctggcgtcg gcgcgggcca
     3061 tctcaatctg ccgttgtgcc cggcggatcc gccgcgcacc atccgatgaa gccgtggccc
     3121 gtggcaggcg atcgccggtc gccgattcga tgtcccggaa cgagcggcca ctggatcgcc
     3181 tgaggcagtg cagtgcggca acccacgctt cacggtgcac cgcgtgctgg tcgacccctg
     3241 cgccatgccg gccggcttat gcgatgcaaa tctgtgggga ggccgctgaa ggctctctca
     3301 gtcgcaaccg ggcattctca cctcaacgac gggaggtggc ggatggccag ggcagcggtg
     3361 gcgcgatgat cgccctcggg gtggtctgcg cggttctcgg ggcggtggcc gccgccttcg
     3421 gggcgtggct gcagcaccgc ggcgtcaccg ccgtcggccg cacccatccg gccgcccttc
     3481 tgcgcaaccc ggcctggcgg ctcggcgtcc tcgtcctcac cgcctgcacc gtcctgcagg
     3541 cactggccct cgcgctcgcc ccggtcaccg tcgtcacccc gatcgtcgtg ctggcgctgc
     3601 cgatcctgac cgtcctcaac ggaccccgcg acccgctcac ccgcgttgcc gtcgccgcgg
     3661 tcacgctcgc cgtcgcggtg ttcgtcaccc tgaccgcgcg cgacgcgaaa cccgccacca
     3721 tcccgccgga caccgtcctg gacgccggtc agctcgtcgg catcatcgtc gtgagcctcg
     3781 tcgccgtcgc ggcgttcgcc cagggcatcg cccgcagcgc ggctctcgcc gtcgccgccg
     3841 gggtcggcta cggcctggtc gccgtgctca tcaaggacgt cctgcacgcc gacggcctgc
     3901 cctggctcag cgtgctcgcc acggtcctcg cgttcgccac cgccgcctgg ctcgtccagc
     3961 acgcctacgc cagcggcccg ccggacgtcg tcgtcgggtg ccagaccgcc gccaaccccg
     4021 tggtcgccac cgggctcggc atgaccgtcc tcggcgaggc gcccgcgctc gacctgccga
     4081 ccctcgccgc gtgcggagcg accgcgatcg ccggcatcgc ggtgctcgcg gtccggcgcg
     4141 acagcctcac cggcgcgcgt gccacgcctt catcgaacag tccaggccgt ccagcagcat
     4201 ggtcaggtac cgctgccaca ggtccggggc cacgtcgtgc gagtactcca cgaccgacgc
     4261 gaccatcttg aacagcaccg gcagtccgtc tcggcgaagt ccccgcgcaa cgcgccgctg
     4321 cgaggaggac gcccgcgcgg cactggccga gccggacccg tgggaggcgg ttcccgaaaa
     4381 aggaacacct gatcgacgcg atgttcgccc gccggctgga ggaaggggag tcgagctcgc
     4441 cgggggaggc cagccggctc ggcggccgat ccgcaccacg cgcggcgctg gctgatcctg
     4501 gtgatgatcg ggctggccca gctgatggtc gtgctcgacg cgaccatcgt gaacatcgcc
     4561 ctgcccaccg cccagcacga gctgggcttc tccagcgacg cccgccagtg ggtggtcacc
     4621 gcctacgcgc tcgccttcgg cagccggctg ctgggcgtgt tcgcgcggat cgagaggcgc
     4681 gtggcgcacc cgctgctgcc gatgcgcgtg ctgtcgtcgg gccgaaggtc ctcgtcggcg
     4741 tctggggcgc gggcatcttc ctgctcggcg cgatcatctg cggactgctg atcaagccgg
     4801 gcgtgcccgc agaactccac acggacatgc cggtcgtcca cgcgtagtgc gcgaagccgc
     4861 cggttctcag catgagcgca cgacggtcgc ggcgagggca catcttccct ggtcggttcg
     4921 cggagtgtga attaacccac gagagtgacg ggtcattcgg taatgtcgaa tcacctttgg
     4981 tatcgcggaa ggagattcgg atgacagtgc cggttgacga ggtgttgacc accacccgtg
     5041 ctgttcgcag gcggctgctg ctcgacgtgc ccgtcgagga ccaggtgatc gaagactgcc
     5101 tcgaagtggc cttgcaggcg cccaacggtg gcaaccgcca gccctggcgg tggatcgtcg
     5161 tgcgggacga gggacagcgg gcacggatcg ccgaggtgta ccggcgctgc ttcgccgatt
     5221 acgtcgccga gctgggcaac cccgggccgt cccgggacct ggacagcgcc cggttcctcg
     5281 ccgacaacct gcaccgcgtc ccggtgctcg tgctgccgtg cctcgtcgcg ccgcggctgc
     5341 ccgagggcag ccaggccagt ccgtgggggt cgctgctccc ggccgcctgg agctacatgc
     5401 tcgccgctcg cgcgcgggga ctcggcaccg cgtggaccac ctgccacctg cgccgggaag
     5461 ccgagatcgc ggacatcctc ggcctgccgc ccgggatccg gcagggtgtc ctgatcccga
     5521 ccgcctacca cgacggcggc ccgttccggc ccgcccgccg gacaccgctc gcggacgtgc
     5581 tggtggtcga ccgctggccc cggcccgagc ggcggacccg tgagctcacg gccgacctcg
     5641 acgacccgtt cttcttcgac cacgaactcg accacctgcc cggcatgctg ctcctgagcg
     5701 ccgcactcga cctcgtacgc ccggccggcg gggaccccgc cgtcaccctg gcactcgact
     5761 tccgcggcat gggggagctg gacgagccgg tgcggctgac cgccgagtct cccgacgaca
     5821 gcggccggct ctggcaggtt cacgccgatc aggacggcag gacgatcatg accggcacca
     5881 tcgagcggat cggcgcggcc gcgagcgcgc ccgggaccgt ggccgggacc gtaaagcccg
     5941 ttgagcagcg gctggtgcac cgccggcgcg cggagaacgt gttcgtcggc gacctcgtcg
     6001 agagcgagca cgactggcgg gtgaccgtgc tgccggcccc agccgggcac cacctcgacg
     6061 gcggccggac cgcggaacgg ctcatcgagg cggcgcggca gttcgtgacc ctcctggagt
     6121 acgcggacaa ccagcggcag gagggcacgc ggttcgtgct gacccgcctc gccgccacgg
     6181 tgcccgccgg tccgtccgcc ggagcggtcc tgagctgggc gaagaccggc tcacgcggca
     6241 accggccacg ctacgacgtg cggatcctcg acgccggcgg ccgggccgtg gggtcggtga
     6301 ccgtctgcgg ccgggcactc agcgacgccg cgttccgcag gctccggggc cggccggcac
     6361 cgctggccac ggctgccccg gttctcagcg ccggcgggcc cgcagcgcct cgatgacctc
     6421 gtcgtcccag cggtgctggc ggatcagccg gtagcgctcg cccgcgaccc acatgtaggc
     6481 ctcggcctcg gtgcggtcgc cgatcagctc cgcctggcgc atcatctcca ccaccggcag
     6541 gtactcctcg tcgtaccagc gccgggcgat cgtggcgcgg tcgaggaact tgccctcgga
     6601 ctgcatgagc cggaagcccc acgcctcgac gtgctcgccg aggtgcgcgt agtcccacgg
     6661 gtcgctcacc acgatcgccg cgcgcgcttc gccgctcaac ggcacccgcg acaggaacag
     6721 ccgccggtag tccttgacga tcaggtcgcc ccggtagcgg atgcccttcg ggttgagctt
     6781 ggtgtgcacc tcggtcaccg acgcctcgat ggtctccagc ccgagcgcgt aggcgaccga
     6841 aacgcggtgg tgcccgtcga tcacgaagtg caggtcgccg acccggtaca cctcgatcgg
     6901 cgggatgtgc tcgccgcggc gggcggccag cgcgagccgt tcccagcgct cccgcacccg
     6961 gccggaggtg ggccggaacc ggcggtcgaa gtcgcggccc cggtccacgc tgccgacgat
     7021 cgagttcagc gggatcaccc gcagcccgac ccgccgctcg ctcacgtagc cgagcgcgtc
     7081 gacgacctcg tcgaacggca gcatgatgtt gacgtcgtcg ggttcccggc gcagccagtt
     7141 cgccagccgg gacagcacct gccgccgccg ggcgcgcagg aagtcgtgtt cggcgtcggc
     7201 gcgggggaag ccggtgtccc gcgcggggtt cacgtccgca cctcgtgcgc ggtgaactcg
     7261 atgaggtgct tgccgacgac gttgcggact ctggtctcgc ccatccggtg ctcccggggg
     7321 tgttcgccgt aggggtggat gtggccgtgc agcaaccagt ccggccgcat ccgctcgacc
     7381 gtggggtgca ggcagtcgaa gccgcggtgc ggcgggtctt cgcggtcgcc gacgccgcgc
     7441 ggcggggcgt gcgtcagcag gacgtccacg ccgcgcccgt cccggcgccg ccgtcgcgcg
     7501 gcctgccgga ccaggcgccg ggcgcggcgg gcctgctgcg cctgggtcca ctggttgggg
     7561 ccgtcgttgt agcggaccga gccgcccagg ccggcgatcc gcaggccgca cacgtcgacg
     7621 acgcggccgt cggcgttgat gccgccggcc gggccgggcc acgcctgcgg gaacccggcc
     7681 cgcaggaaca ggccgccgtg gcgggtgaac ccggacaggt ccgggtcgtg gttgccgggc
     7741 acgaacacgc acggcacgcc gcccagcgcg tcggtcaggt actccaggta gtcgaacggc
     7801 aggtcaccgg cggccaggac gaggccgacg tcgcagccgg ggccgatacc ggcccacagc
     7861 cgctcgtcga cctcgtcggc gaccaccagt gcgcgcatgg gtccacgata gggactcctc
     7921 agcgcgcctg cgcgagttcc gggtggtcgg cgatcaggac cgccaccacc gcgatccacg
     7981 tcagggtcag cgcgagggcc cagaacttca ggctcgagag cagtctggtc atggagcgac
     8041 tccttccatc gaataggtca ggaatgggct agagccagtt gttcttcctg aatattcggt
     8101 aaagaagcag gcagatcccg agaatcatga cgagaaccgc cggatagccg aactgccagt
     8161 gcaactccgg catgaagtcg aaattcatgc cgtagatacc cgcgatcata gtgggcaccg
     8221 tgatcatcgc cgcccacgag gtgatcttgc gcatgtcgct gttctgctgg agcgtgatct
     8281 tggcgaccgt cgcgttcacc agcgtcgtca gcagctcgtc gaagttgccc acccgctccg
     8341 acacggtggt caggtggtcg tcgacgtccc ggaagtagga ccggacctcg tcgggcacca
     8401 gccgggtgaa gccctcggcc agccggcgca ccggcgtgac cagcggcatg accgcgcggc
     8461 gcagctccag cacctcgcgt ttcatgaggt agatctgctc cgcgctgacc gtcgaccgcg
     8521 gcgcgaacac gcgcgcctcc atctcgtcga tgtcctcctc gatcgaggag gtgacgtcga
     8581 ggtactggtc gacgatgagg tcggtgatgg cgtgcagcac cgccgccggc ccgttcacca
     8641 gccgctccgg gtcctcgtcg aggtcgcggc gcagccgggc cagcccggag tgcgccccgt
     8701 gccggacggt gatgatgaag tccagcccga ggaacgccat cagctcgccg gtctccacga
     8761 tctcgttggc cgtggtcggg gagtcgtgct cgacgtagcg gaccgtcttg agcaccatga
     8821 acagcgtgtc gtcgtagcgc tccagcttgg gccgctggtg cgcctgcacg gcgtcctcga
     8881 cggccagctc gtgcaggccg tagacctccg cgatgccctg gatctgctgc tggtcgggct
     8941 cgtgcaggcc gatccagacg aagccatcgc gccggcgccg gacctccttg atggcctcgg
     9001 cgtgcgtcca gcgcccgggc agccgttcgc cgtcgacgta gaccgcgcag tcgacgatgt
     9061 aggccgacac cggcaccggc acgggcggct cgacgcgcgc ggcgcggccg ttgccccggc
     9121 cgcgcaggcc accgagggag ggaatcgcag gcatgggatc tcctggcttt cgcagtgcgt
     9181 accacgacag ccgggcgggc aagaacacgt ccgctcggct ctaccagcgt ttcccgggcg
     9241 aaaagaggcc taacggccgg aaacggatcc agccgtgcgg agggagacgc tgggtttact
     9301 agggagcgga ctatcgccac tactcatcgt gcgttcctca cctcctccag ccggaccggc
     9361 aacgtggcgg ctgtcgcgtt gacgcaccgc cgccattggt cgagggtact cctcaacatc
     9421 agttgacgtc gctccaggtc ggtgtggcgt tacccgcagg gaggtcgagc ggtgcagttc
     9481 gggcggtatt tcgaggagtt cgaggtgggc gcggtgtaca agcactggcc cggcaagacg
     9541 gtgaccgagt acgacgacca cctgttctgc ctgctcacca tgaaccacca cccgctgcac
     9601 atggacgcgc actacgcggc cgagaccacc gacttcggca agaacgtggt ggtgggcaac
     9661 tacatctact cgctgctgct ggggatgtcg gtgccggacg tgtcgggcaa ggcgatcgcg
     9721 aacctggaga tcgaatcgct ccggcacgtc aagccgacct tccacggtga caccatctac
     9781 ggtgagaccg aggtactgga gaagaccccg tcgaagtcca aggacgaccg gggcgtggtg
     9841 tacgtggaga cccggggcta caagcaggac ggcaccatcg tgtgcatctt ccggcgcaag
     9901 gtcctggtgc cgaagcagaa ctacggtgag gcgcgtgggg gcgagcagcc gggccgtccc
     9961 gttccgcacg agtgagggag agcgcgtgac gatcgggttg gccgaggcca agcggcagct
    10021 gaccgacttc gccgggagcg cggcgggcga gtcgccgctc tacgagcacc tcgcgtcgaa
    10081 ggccgccggg gacgacgagg tggccggcct gctggccgcc gcgcccggcg gggcgagccc
    10141 ggcgctgctg ttcgcggtcg cgcaccggct catccaggcc gacccgatcc acccgctctc
    10201 gcgctactac ccgtcgctgg gcgggttcga cggcgtcgac ggccagacct ggccggtgtt
    10261 ccgcgagttc ctgctcgcgc acgccgacga tgcggccggg atgctcgcgt cgaagtcgct
    10321 ggcgaacaac gaggtgcgcc gggccgcgtc ggcgttcccc ggggtcgcga tggccgccaa
    10381 gcaggccggc ggcaagatcg cgttgatcga ggtcggcacc gcgggcgggc tgctgctggg
    10441 gatggacaag ttcgcctacc ggtaccagtg cgacggcggc gagcagctgg tggccggtcc
    10501 ggccaaggcg gccgtcgggc tgcactgcgc gctcgacctg gcgcccggcg cggtgctgcc
    10561 gaaactgccg aagaagctgg ccgtcgcggc gcgcgtcggg ctcgacccgg cgccgctgga
    10621 tctgtccgat gaggacgagc tggcgtggct ggaggcgtgc gtgtgggccg accagccgga
    10681 ccgggtgcgg ctgctgcggg cggcggcgct ggcgcagcgc aagtcgccgc cggagatcat
    10741 cgccgctggc gcggcgggcg ggttcggcga ggcggtgtcc cgggtgcccg cggacctgcc
    10801 gctggtggtg ctcaccagcc ggacgatggg ccacatcccg gacgccgaac gcgccgagtt
    10861 cctgtccagc atggccgcgc tgggcgagtc gcgtccactg tggtgggtca tggacgagac
    10921 gtacgagcac gggttgtccg cggtgctgcc gggccgggac gagctggctt tcgcccgcga
    10981 cggcctgacc acgctggggc tcgcccggcg caccgcggac ggctgggacg cgctcgccct
    11041 ggcccgcacc acgcgctacg gcgcccggat gacctggctg gccgcctagc tgttgtgtgg
    11101 ccggggccac tgcggtgagg atgtggtcat ggctgaggac acgtggttca ccccgctcac
    11161 cccgctggcg ttcctggagc gctccgccga ggtcttcgct gacaaggagg ccatcgtcta
    11221 cggcgagcgc cgggtgacct accgcgagtt cgccgccgag gtgacgcgcg tggcgcgggc
    11281 gctgcgggcg agcggggtcg agcccgggga ccgcgtcgcc tacctcctgc cgaacatccc
    11341 ggagatgctg gtcgcgcact tcgcggtgcc gctcgccggc gcggtgctgg tcgcgatcaa
    11401 cacccggctc gcgccg
//
