LOCUS       NZ_BNAU01000008        10915 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Amycolatopsis deserti strain CGMCC 4.7677 sequence08, whole genome
            shotgun sequence.
ACCESSION   NZ_BNAU01000008
VERSION     NZ_BNAU01000008.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Amycolatopsis deserti
  ORGANISM  Amycolatopsis deserti
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 345420)
  AUTHORS   Sun,Q. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 82X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014653945.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:43:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,561
            CDSs (total)                      :: 7,498
            Genes (coding)                    :: 7,407
            CDSs (with protein)               :: 7,407
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 32 of 91
            Pseudo Genes (incomplete)         :: 64 of 91
            Pseudo Genes (internal stop)      :: 8 of 91
            Pseudo Genes (multiple problems)  :: 12 of 91
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:49:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 85412
            Orig. end                         :: 96327
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNAU01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10915
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10915
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10915
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5915](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5915)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(338..350)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     gene            472..1551
                     /gene="hemE"
                     /locus_tag="IE290_RS29620"
                     /old_locus_tag="GCM10017786_60130"
     CDS             472..1551
                     /EC_number="4.1.1.37"
                     /GO_function="GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671908.1"
                     /locus_tag="IE290_RS29620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60130"
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="WP_191247991.1"
                     /transl_table=11
                     /translation="MSTPAQSRPRTAHRDLTGSPFLAAARGHRPARLPVWFMRQAGRSL
                     PEYRALREGVPMLKACFDPEMIAEITLQPVRRLGVDAAVFYSDIVVPLVAAGIDVDIVP
                     GTGPVVAEPVRDAAAVRRLPALDPERLEPVLDGARLLVERLGQTPLIGFAGAPFTLASY
                     LIEGGPSRSFERTKALMRSEPEVWHDLAGRLADIAVTFLRGQIEAGADVVQLFDSWAGA
                     LTLREYREFVLPHSARVLGAVADLDVPRIHFGVGTGELLTAMREAGADVVGVDWRVPLD
                     EAVRRLTAHGEPVPVVQGNLDPALLQASWPVIESEVRRIAAEGRAAAAHIFNLGHGVPP
                     GTDPDVLARVVELVHSLEG"
     gene            1551..2918
                     /gene="hemG"
                     /locus_tag="IE290_RS29625"
                     /old_locus_tag="GCM10017786_60140"
     CDS             1551..2918
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422378.1"
                     /locus_tag="IE290_RS29625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60140"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_191247992.1"
                     /transl_table=11
                     /translation="MHVAVVGGGVSGAVAAYRLRAALGDGAAITVVEATSALGGKLRTA
                     EIAGVRYDVGAEAFLARRPEGVGLAGELGLELVHPTKARSTVRAGGRLAPLPGRTLMGI
                     PGSPDAVTDVLSDAGRARVAAEPELPPVTLESDTALGALLRERFGDELVDRLVDPLLGG
                     VYAGGADGLGLRATVPALATAIDRGAPSLTAAAAGLIPATPSTAPVFGTLPGGLGTLID
                     RLVELAKPDIRLGQPVTALRRRENGWRLEVGDESIDADAVLLAVPAPAARRLLADVVPA
                     ASSALGEVELASMAVVAMALPPGTELPDASGVLIGARERRSDGEPFAAKAFTFSSRKWS
                     HLDTGPVLVRGSVGRFGKPGALRRDDDELVRLVRDDLAELTGITAQPIDVAVQRWGGGL
                     PQYGPGHLDLVERAERAVAAVPGLALAGAALHGVGVPACIATATAAATRITSQLVR"
     gene            2952..3644
                     /gene="hemQ"
                     /locus_tag="IE290_RS29630"
                     /old_locus_tag="GCM10017786_60150"
     CDS             2952..3644
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="IE290_RS29630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60150"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_191247993.1"
                     /transl_table=11
                     /translation="MARLNYQELNDTIRYTAWSVFRVEPGRLPEDRGQAATETTEYLDA
                     LEGKGVVVRGVYDVAGLRADADYMIWWHAETPEQVQAAYTGFRRTPLGRVSTPVWSQFA
                     LHRPAEFNRSHIPAFLAGEEARKYICVYPFVRSYEWYLLPDAERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARRHVREEIPFYTGTKVVP
                     AELILNLP"
     misc_feature    2994..3009
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.76"
                     /tool="antismash"
     gene            3921..4649
                     /locus_tag="IE290_RS29635"
                     /old_locus_tag="GCM10017786_60160"
     CDS             3921..4649
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (8-156). E-value: 4.6e-12. Score:
                     38.2. Matches aSDomain:
                     nrpspksdomains_IE290_RS29635_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948410.1"
                     /locus_tag="IE290_RS29635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60160"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_191247994.1"
                     /transl_table=11
                     /translation="MPGRLDGKVALITGASGGIGAATAALFAREGARLVLGDVTGSGPE
                     REDVLRVHLDVSSARDWQAALARTAERFGRLDVLVNVAGIVDWPGIEDTTESGWDQVIA
                     VNQKGTWLGMKTAMPLLRASGNASVVNVSSILGIIGGGSAAAYHASKGAVRLLTKTAAV
                     EYATRGVRVNSVHPGVIATPMIQDILDAEGDQQADIQRTPMKRAGTPDEIAAAILFLAS
                     DESSFVTGAEIVVDGGITAH"
     aSDomain        3945..4388
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE290_RS29635_PKS_KR.1"
                     /evalue="4.60E-12"
                     /label="IE290_RS29635_PKS_KR.1"
                     /locus_tag="IE290_RS29635"
                     /protein_end="156"
                     /protein_start="8"
                     /score="38.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALITGASGGIGAATAALFAREGARLVLGDVTGSGPEREDVLRVH
                     LDVSSARDWQAALARTAERFGRLDVLVNVAGIVDWPGIEDTTESGWDQVIAVNQKGTWL
                     GMKTAMPLLRASGNASVVNVSSILGIIGGGSAAAYHASKGAVRL"
     CDS_motif       3951..4028
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE290_RS29635_0001"
                     /evalue="1.20E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE290_RS29635"
                     /protein_end="36"
                     /protein_start="10"
                     /score="17.9"
                     /tool="antismash"
                     /translation="LITGASGGIGAATAALFAREGARLVL"
     gene            complement(4675..5004)
                     /locus_tag="IE290_RS29640"
                     /old_locus_tag="GCM10017786_60170"
     CDS             complement(4675..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983666.1"
                     /locus_tag="IE290_RS29640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60170"
                     /product="hypothetical protein"
                     /protein_id="WP_191247995.1"
                     /transl_table=11
                     /translation="MSREELAARQAELLRALLAGGAPPAGFDPGRLAAEVLALRAKRRS
                     IVADLRPDLFRMLGDRFHPLFDAYAEAAPRSDGTGYRQDSANFAAWLADRGELPRRRWW
                     SRSTR"
     gene            complement(5001..5915)
                     /locus_tag="IE290_RS29645"
                     /old_locus_tag="GCM10017786_60180"
     CDS             complement(5001..5915)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677307.1"
                     /locus_tag="IE290_RS29645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60180"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_229874826.1"
                     /sec_met_domain="DUF692 (E-value: 2.3e-85, bitscore: 280.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGVGIGWRPEIDLSIARLPGVEWVEVIAENLHPGHLPGSLVE
                     LRERGLPVLPHAVSLSLGGAEPLDTRRVEHLAAVADELDAPLASDHVCFVRAGGLDAGH
                     LVPLPRTREALDVLVENVRLAQSIVGRPFALENIAALLEWPDAEMSEAAFLAELTSRTG
                     CLLIVDVANLYANARNLGADAVEFLDEVPLERVAYVHVAGGIEVDGLYHDTHTHPVLPE
                     VLDLLGELRRRTEPPGALLERDGAYPPDAELAAELAAIRERLGGAEPLHHAKRPQERLG
                     AAEPPAIRGPVPQAEPGATRERL"
     gene            complement(5911..6825)
                     /locus_tag="IE290_RS29650"
                     /old_locus_tag="GCM10017786_60190"
     CDS             complement(5911..6825)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422373.1"
                     /locus_tag="IE290_RS29650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60190"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_229874787.1"
                     /transl_table=11
                     /translation="MAGPVLLSLGDGPAFWGLPASTFLPVFAVLVVLPVLVRTLGSRLL
                     KRRDGGPHRPPGPVELGFLAGGPARATDAALAGLLEQGRVRVSSSGRLSTTGGRAPADD
                     LTGAVWTSVQFGRGTWTVHEVRTEMLGRAEFEQLRDRLTGEGLLVERGPMRVVRRLAMV
                     LLVAVVVIAFVTFPHGTFVLAVLPAGLLWTLTDRPARGPARTTTGDAALRAASVTPGGA
                     ARDVALGGLSRYPDAGIAQALLSPPPPQKPRRRWGSGSSSGGAYGYGCGSSGSSCGSSS
                     CGGSSCGGGSSCGGGGCGGGGGD"
     gene            complement(6830..7516)
                     /locus_tag="IE290_RS29655"
                     /old_locus_tag="GCM10017786_60200"
     CDS             complement(6830..7516)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="IE290_RS29655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60200"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_191247996.1"
                     /transl_table=11
                     /translation="MGAYWGLSARELVVLYAALILVPPVVRLLGPVLLRCRRIRIGADR
                     VTPAELAALAGGAERAVDFVLADLVARQWIRVDGTGRLTTTSQRAPDDPLAVAVVLAVS
                     FWRGLATARTLRVYLAKGTPFEQLVGGLAARGLAVAPGDAAGIRIACRVLLVGVVASTF
                     AGGYAVAAIPVMLGGVLVLGLAELVPPPRRTIAGSRLLRRVSRPPGNRGHVPLVESPTR
                     PWGTWR"
     gene            complement(7572..7994)
                     /gene="msrB"
                     /locus_tag="IE290_RS29660"
                     /old_locus_tag="GCM10017786_60210"
     CDS             complement(7572..7994)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422371.1"
                     /locus_tag="IE290_RS29660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60210"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_191247997.1"
                     /transl_table=11
                     /translation="MEPVVGATPKVVKSDQEWREQLSPEEYAVLRQAGTERPFTGEYTD
                     TKTTGVYECRACGAELFRSDTKFESHCGWPSFFDPSSSDAVLLREDRSLGMVRTEVLCA
                     TCHSHLGHLFEGEGYPTPTDQRYCINSISLRLVPAE"
     gene            complement(8017..8307)
                     /locus_tag="IE290_RS29665"
                     /old_locus_tag="GCM10017786_60220"
     CDS             complement(8017..8307)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983661.1"
                     /locus_tag="IE290_RS29665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60220"
                     /product="hypothetical protein"
                     /protein_id="WP_191247998.1"
                     /transl_table=11
                     /translation="MTESGRNPRFDDDLVGLDPDDPEAQAFARHLDRMQRCEPGFTVEG
                     TLHNVAEFAESSARVGGLRWWIAATIVVLLVLGVIVASWDIVVRAVQWLAE"
     gene            complement(8323..9864)
                     /locus_tag="IE290_RS29670"
                     /old_locus_tag="GCM10017786_60230"
     CDS             complement(8323..9864)
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157015.1"
                     /locus_tag="IE290_RS29670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60230"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_268246419.1"
                     /sec_met_domain="Pkinase (E-value: 2.6e-56, bitscore:
                     185.2, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIAGHYRLVEHIGSGAMGVVWRAVDERLERSVAVKQILAQPGMSE
                     AERNSMRQRAMREAKNAARFQHPNAIVVFDIAEHGGDPCLVMEYLPSRSLSAVIAEQGT
                     LPLAEVARIGHQVASALVVAHRAGIVHRDIKPANILIDENGTAKITDFGISRAAGDLTL
                     TQTGLIGGTPAYLAPELARGSDPAPASDVFSLGATLYHALEGQPPYGNSSNQLALLYTA
                     ANGQVIPPRSSGKATALLMSLLRVEPEERPTMLEARDRLAALANGEKDTKGGTMVSPPL
                     FSSGRQPAAVPAEAPAPAERPPWQRTGAATAAPPAPPQRSPRNPTAAFIPPPKPPQQAK
                     PSPLKRKGVLIGSAVAVVAVLVVVLLVALNGGDTANGGTSQANVGGPTPTPSAPSSTSS
                     SAAPADLGKTANTGSVDFGSAGQVVKAYLDGSGSAQAWNLLTPAAQEVFGSQQAFQQYW
                     SEHKIQGYSSIRADNGANADGSADMYVSIDGVGRISWRVVSTSGGLRIDSDTKLG"
     gene            complement(9884..10612)
                     /locus_tag="IE290_RS29675"
                     /old_locus_tag="GCM10017786_60240"
     CDS             complement(9884..10612)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983659.1"
                     /locus_tag="IE290_RS29675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_60240"
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="WP_229874789.1"
                     /transl_table=11
                     /translation="MSRRLKLIVALVLLAGAVVAAFTLPVPSPAELRNWAAGAGAVTAL
                     VFLAAYAVLTVAPIPRTVFNLAAGLLLGDVLGIVVAITATAVSGALGFGLARLVGRDLV
                     ARHLERKVVRAVDERLADGGILAVASLRLIPLVPFAPLGYCCGILSVRFRPYLIGTVLG
                     SLPGTIAVVVLGDALTGSTPPALLACYGAFAVAGAVGLARIARGTANAGRIRQPATLDP
                     VREEIVRAEPSQHMRRVSEE"
     misc_feature    complement(10606..10620)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.23"
                     /tool="antismash"
ORIGIN
        1 cgacgaggtc tcggtgaccg tgccgcccag cgcggtgaac gccgccccgg actgctccag
       61 tgcgtcggtg agccacgacc agccgaccgc cggcaggaac gggtcgctgg ccagttcccg
      121 gtcgatctcc gcgcgcacgt agacgaccac ccgcagcacc ccgttccagg cctcctggcc
      181 ctccggatcg tgcagcagca ccagccggcc ggtggccgtc acgtccgcgg gacccgtcgc
      241 gtcgcagctg agcgcgtagg accagggcgc caggcgctgc ggcgcgcgca tgggttcgag
      301 ggtcatctcc ggccggggcc ggatcgcttg caacgccgcg acagcttcac ggaacatctc
      361 gggcgcatga gtcatcgcgg tcacagcccg actgtaggac ggggagcggg tgccgggcgg
      421 cagacgcgcc gagggaaggc gccgccgggc cgcgtggcac catggttgac gatgtccact
      481 cctgcgcagt cgcggccacg cacggcccac cgggacctca ccgggtcccc cttcctcgcg
      541 gccgcccgcg gccaccgtcc cgcgcggctg cccgtctggt tcatgcgcca ggccggccgc
      601 tcgctgcccg agtaccgcgc gctgcgcgag ggcgtgccga tgctcaaggc gtgcttcgac
      661 ccggagatga tcgccgagat cacgctccag ccggtccgcc gcctcggcgt ggacgccgcg
      721 gtcttctaca gcgacatcgt ggtcccgctg gtcgccgcgg gcatcgacgt cgacatcgtg
      781 cccggcaccg gtccggtggt cgccgagccg gtgcgcgacg ccgcggcggt ccgccgtctg
      841 cccgcgctgg acccggagcg gctcgagccg gtgctggacg gcgcccggct gctggtcgag
      901 cggctggggc agaccccgct gatcgggttc gccggcgcgc cgttcacgct cgcctcctac
      961 ctcatcgagg gcggccccag ccgcagcttc gagcgcacca aggccctcat gcgctccgag
     1021 ccggaggtgt ggcacgacct ggccggacgg ctcgccgaca tcgcggtgac cttcctgcgc
     1081 ggccagatcg aggccggcgc cgacgtcgtg cagctgttcg actcctgggc cggcgcgctg
     1141 acgctgcgcg agtaccgcga gttcgtgctg ccgcactcgg cgcgcgtgct cggcgcggtc
     1201 gcggacctgg acgtgccgcg gatccacttc ggtgtcggca ccggcgagct cctgaccgcc
     1261 atgcgcgagg ccggggccga cgtggtcggc gtcgactggc gcgtcccgct ggacgaggcc
     1321 gtgcggcggc tgaccgcgca cggcgagccg gtgccggtgg tacagggcaa cctggacccg
     1381 gcgctgctgc aggcgtcctg gccggtgatc gagtccgagg tgcggcgcat cgccgccgag
     1441 ggccgcgccg ccgccgcgca catcttcaac ctcggccacg gcgtgccgcc cggcaccgat
     1501 cccgacgtgc tggcccgcgt cgtcgagctc gtccacagcc tggagggctg atgcacgtcg
     1561 cggtcgtcgg cgggggcgtc tcgggcgcgg tcgcggcgta ccggctgcgt gccgcgctgg
     1621 gcgacggcgc ggcgatcacc gtcgtcgagg ccaccagcgc gctcggcggc aaactgcgca
     1681 ccgcggagat cgcgggcgtg cgctacgacg tgggcgccga agcctttctc gcccgccgcc
     1741 cggaaggcgt cgggctggcc ggggaactgg gcctggagct ggtccacccg acgaaggccc
     1801 gctcgaccgt gcgggccggc ggacggctcg cgccgctgcc cggccggacg ctgatgggca
     1861 tccccggctc gccggacgcc gtcaccgacg tgctttccga cgcgggccgc gcgcgggtcg
     1921 cggccgagcc ggagctgccc ccggtgacgc tggagtccga caccgcgctg ggcgccctgc
     1981 tgcgcgagcg cttcggcgac gaactggtgg accgcctggt agacccgctg ctcggcggcg
     2041 tctacgcggg cggcgccgac ggcctgggcc tgcgcgccac cgtccccgcc ctcgccaccg
     2101 cgatcgaccg cggcgcgccc tcgctgaccg cggccgcggc cgggctgatc cccgccacgc
     2161 ccagcaccgc gccggtgttc ggcaccctgc ccggcgggct cggcacgctg atcgaccggc
     2221 tcgtcgagct ggccaagccg gacatccgcc tcggacagcc ggtgaccgcg ctgcggcggc
     2281 gggagaacgg ctggcgactg gaggtcggtg acgagtcgat cgacgccgac gccgtcctgc
     2341 tcgcggtgcc cgccccggcg gcccgtcggc tcctggctga cgtcgtgccg gccgcgtcgt
     2401 ccgcgctcgg cgaggtcgag ctggcgtcga tggcggtcgt cgcgatggcc ctgccgcccg
     2461 gcaccgagct gccggacgcc tccggggtgc tgatcggggc gcgcgaacgc cgctccgacg
     2521 gcgagccgtt cgccgcgaag gccttcacct tctcctcccg caagtggtcg cacctggaca
     2581 ccggcccggt gctggtgcgt ggttcggtcg gccggttcgg caagcccggc gcgctgcggc
     2641 gcgacgacga cgagctggtc cgcctggtcc gcgacgacct ggccgagctg accgggatca
     2701 ccgcgcagcc gatcgacgtc gccgtccagc gctggggcgg cgggctgccg cagtacgggc
     2761 cgggtcacct cgacctggtc gagcgggccg agcgggccgt cgccgcggtg ccggggctgg
     2821 cgctggccgg ggccgccctg cacggtgtcg gcgtgccggc gtgcatcgcg acggccaccg
     2881 cggccgcgac ccgcatcacc tcacagctgg tgcgctagca gcggctttcc gggcagtgcc
     2941 aagatggggg catggcgcgg ctgaactatc aggagctcaa cgacaccatc cgctacaccg
     3001 cctggtcggt gttccgggtc gagccagggc ggctgcccga ggaccgcggc caggccgcga
     3061 cggagaccac cgagtacctc gacgccctgg agggcaaggg ggtcgtggtg cgcggcgtct
     3121 acgacgtcgc cgggctgcgc gccgacgccg actacatgat ctggtggcac gccgagacgc
     3181 ccgagcaggt gcaggccgcc tacaccgggt tccgccgcac cccgctgggc cgggtgtcga
     3241 cgccggtgtg gagccagttc gcactgcacc gccccgcgga gttcaaccgc agccacatcc
     3301 ccgccttcct ggccggagag gaggcgcgga agtacatctg cgtgtacccg ttcgtgcgct
     3361 cctacgagtg gtacctgctg ccggacgccg agcggcgcaa gatgctcgcc gaccacggca
     3421 aggaggcccg ggactacccg gacgtccggg cgaacacggt ggcgtccttc gcgctcggcg
     3481 actacgagtg gatcctcgcc ttcgaggccg acgagctgca ccgcatcgtg gacctgatgc
     3541 gccacctgcg cggcaccgag gcgcggcggc acgtgcggga ggagattccg ttctacaccg
     3601 gcaccaaggt cgtgccggcc gagctgatcc tgaacctgcc gtaggttttc gacgcgcccg
     3661 gccccggtcg agtttcacca gcgcggaggc catgccggcc gttcgggagg tggcggccgc
     3721 gggggatcga tcccagggtg accggtagtg ggttcgggtt ggtgcgtcac gtgacttgtc
     3781 cgggtgagtc gtgccgagtc cgatgtggac taccgggccg ccgctgccgg ggagttcggt
     3841 cctgggagta gtgcaccctg gttggcccgc tcatgtcgcc gcacgatggg gaggtggtgg
     3901 cagcggtagg agggagcggg atgccgggac ggctggacgg caaggtcgcg ttgatcacgg
     3961 gcgcgtcggg tgggatcggg gcggccacag cggccctgtt cgcccgggag ggggcgcggc
     4021 tggtgctggg tgatgtcacc gggagcgggc cggagcggga ggacgtgctg cgcgtgcacc
     4081 tcgacgtctc ctcggcgcgg gattggcagg cggctctggc caggacggcg gagcgcttcg
     4141 gccggctgga cgtgctggtc aacgtcgcgg gaatcgtgga ctggccgggc atcgaggaca
     4201 ccaccgagtc cggctgggac caggtgatcg cggtgaacca gaagggcacc tggctgggca
     4261 tgaagaccgc gatgccgttg ctgcgcgcga gcgggaacgc gtcggtggtc aacgtttcct
     4321 cgatcctggg catcatcggc ggcgggtcgg ccgcggcgta ccacgcttcg aagggcgcgg
     4381 tgcggctgct gaccaagacg gcggccgtcg aatacgcgac gagaggggtg cgggtcaact
     4441 ccgtgcatcc cggcgtcatc gcgactccga tgatccagga catcctcgac gccgagggcg
     4501 accagcaggc cgacatccag cgcaccccga tgaaacgggc aggcacaccg gacgagatcg
     4561 ccgcggcgat cctgttcctc gcctccgatg agtcgtcctt cgtcacgggc gcggagatcg
     4621 tcgtcgacgg cggcatcacg gcgcactagc cagccggtcg acgccgtgag ctgctcagcg
     4681 cgtggagcgg gaccaccagc gccgccgggg cagttcaccg cggtcggcga gccacgcggc
     4741 gaagttcgcg gagtcctggc ggtagccggt gccgtcggag cggggtgcgg cctcggcgta
     4801 ggcgtcgaac agggggtgga atcggtcgcc gagcatccgg aagaggtcgg gcctgaggtc
     4861 ggcgacgatg gagcggcgct tggcccgcag ggcgagcacc tcggcggcca accggccggg
     4921 gtcgaagccc gccgggggag ccccgccggc cagcagcgcg cgcagcagct cggcttggcg
     4981 ggcggcgagc tcttcgcggc tcacagccgc tcccgggtcg cgcctggctc ggcctgcggc
     5041 actggcccgc ggatcgcggg tggctcggcg gcgcccagtc gctcctgggg gcgtttggcg
     5101 tggtgcagcg gctccgcgcc gcccagccgc tcccggatcg cggccagttc cgcggccagc
     5161 tcggcgtccg ggggatacgc accgtcgcgc tccaggaggg ctcccggggg ctccgtccgc
     5221 cggcgcagtt cgcccagcag gtccagcacc tcgggcagca ccgggtgcgt gtgcgtgtcg
     5281 tggtagaggc cgtcgacctc gatgccgccc gccacgtgca cgtacgccac ccgctccagc
     5341 ggcacctcgt ccaggaactc caccgcatcg gcgcccaggt tccgcgcgtt cgcgtacagg
     5401 ttcgccacgt cgacgatcag caggcacccg gtccgcgacg tcagctcggc caggaacgcg
     5461 gcctcgctca tctccgcgtc cggccactcc agcagcgcgg cgatgttctc cagcgcgaac
     5521 ggcctgccca cgatcgactg cgccagccgc acgttctcca ccagcacgtc gagcgcctcc
     5581 cgcgtgcggg gcagcggcac caggtgcccc gcgtccagcc cgcccgcgcg cacgaagcac
     5641 acgtggtcac tggccagcgg cgcgtccagc tcgtcggcga ccgccgccag gtgctccacc
     5701 cgccgcgtgt ccagcggctc cgcgccaccc agcgacagcg acaccgcgtg cggcagcacg
     5761 ggcaagccgc gttcgcgcag ctccaccagc gacccgggca ggtgccccgg gtgcaggttt
     5821 tccgcgatca cctcgaccca ctccacaccc ggcaaccggg cgatcgacag gtcgatctcg
     5881 ggccgccacc cgatccccac gcccagccgc tcaatcaccg ccgccaccgc cgcaaccacc
     5941 gccgccgcac gagctgccgc cgccacacga gctgccaccg caggagctgc tcccgcacga
     6001 gctgccggag gacccgcagc cgtagccgta cgcgcctccc gaggacgaac cggaccccca
     6061 gcggcgtcgc ggcttctgcg gcggtggcgg cgacagcagg gcctgcgcga tgcccgcgtc
     6121 cgggtaccgg ctcagaccgc ccagcgccac gtcccgcgcg gccccgcccg gggtcacgct
     6181 cgcggcccgc agcgccgcgt cacccgtcgt cgtccgggcc ggcccgcgcg ccgggcggtc
     6241 ggtgagcgtc cagaggagcc cggccggcag cacggcgagc acgaacgtgc cgtgcgggaa
     6301 cgtcacgaac gcgatgacca cgaccgcgac caacaggacc atggccagcc gccggacgac
     6361 gcgcatcggg ccccgttcga ccaggagccc ctccccggtg aggcggtcgc gcagctgctc
     6421 gaactcggcg cggccgagca tctcggtgcg cacctcgtgc acggtccagg tgccgcgacc
     6481 gaactgcacg ctggtccaga ccgccccggt caggtcgtcg gccggggcac ggccaccggt
     6541 cgtggacagc cgcccggagc tgctcacccg gacccgcccc tgctcgagca gaccggccag
     6601 cgcggcgtcg gtcgcccgcg ccggaccgcc ggccagaaag cccagctcga ccggccccgg
     6661 cggccggtgc gggccgccgt cgcgccgctt cagcagccgc gagccgagcg tgcgcaccag
     6721 gacggggagc accaccagca cggcgaagac cggcaggaac gtcgaagcgg gcagacccca
     6781 gaatgcgggc ccgtcaccga gtgaaaggag cactggtccg gccaccccat cacctccaag
     6841 tcccccacgg gcgcgtcggc gattcgacga gcggcacgtg cccccggttc cccggcggcc
     6901 gggaaacccg gcgcagcagc cgggaccccg cgatcgtccg ccgcggcggc ggcacgagtt
     6961 cggccagccc gaggacgagc acgccgccca gcatcaccgg gatggccgcc accgcgtaac
     7021 cgccggcgaa cgtgctggcc acgaccccga ccagcaggac ccggcaggcg atccggatgc
     7081 cggcggcgtc accgggcgcc accgccaggc cgcgggccgc gaggccgccg acgagctgct
     7141 cgaacggggt gcctttcgcc aggtagacgc gcagggtgcg cgcggtcgcc aggccgcgcc
     7201 agaacgagac cgccagcacg acggcgacgg ccagcggatc gtccggagcc cgctggctcg
     7261 tggtggtcag ccgcccggtg ccgtccaccc ggatccactg gcgcgcgacc aggtccgcga
     7321 gcacgaagtc gaccgcgcgc tccgccccgc cggccagggc ggccagctcc gccggggtga
     7381 cccggtcggc gccgatccgg atgcggcggc agcgcagcag caccgggccg agcagccgca
     7441 ccaccggcgg caccaggatc agcgccgcgt agagcaccac cagttcccgg gcggacagtc
     7501 cccagtacgc ccccatgcgc ccgcagcgta accggcgggc gtccgccgcg gtggcgcggt
     7561 gcgggatttc gtcactcggc ggggaccagc cgcagggaga tcgagttgat gcagtaccgc
     7621 tggtcggtgg gggtcgggta gccctcgccc tcgaagaggt ggcccaggtg gctgtggcag
     7681 gtcgcgcaca gcacctcggt gcgcaccatg cccagcgacc ggtcctcgcg cagcagcacg
     7741 gcgtcggagg aggacgggtc gaagaacgac ggccagccgc agtggctttc gaacttggtg
     7801 tcgctgcgga acagctccgc accgcaggcg cggcactcgt agacgccggt ggtcttggtg
     7861 tcggtgtact cgccggtgaa cggccgctcg gtgcccgcct ggcgcagcac ggcgtactcc
     7921 tcgggggaga gctgctcgcg ccactcctgg tccgacttga ccaccttcgg ggtggctccc
     7981 acgacgggtt ccatgcgagg tttcatatac gtcaggctac tcggcgagcc actgcacggc
     8041 ccgtaccacg atgtcccacg aggcgacgat cacgcccagg accagcagca ccacgatggt
     8101 cgcggcgatc caccagcgca ggccgccgac ccgggcgctg gactcggcga actcggccac
     8161 gttgtgcagg gtgccctcga cggtgaaccc cggctcgcac cgctgcatcc ggtccaggtg
     8221 cctcgcgaac gcctgggctt ccggatcgtc ggggtcgagc ccgaccaggt cgtcgtcgaa
     8281 gcgcgggttg cggccgcttt cggtcatacc gaccagggta ggtcagccga gcttggtgtc
     8341 ggagtcgatc cgcagtcccc cggaggtgct gaccacccgc caggagatgc ggccgacccc
     8401 gtcgatcgag acgtacatgt cggcggagcc gtcggcgttg gcgccgttgt ccgcgcggat
     8461 cgagctgtag ccctggatct tgtgctcgct ccagtactgc tggaaggcct gctggctgcc
     8521 gaacacctcc tgcgcggccg gggtgagcag gttccacgcc tgggccgatc cggaaccgtc
     8581 caggtaggcc ttgaccacct ggcccgccga gccgaagtcg accgatccgg tgttggccgt
     8641 cttgcccagg tccgccgggg cggcgctgct ggaggtgctg gacggcgccg acggggtggg
     8701 tgtggggccg ccgacgttgg cctgcgacgt gccgccgttc gcggtgtcgc cgccgttgag
     8761 cgcgaccagc aggaccacga cgagcaccgc gaccacggcc accgcggacc cgatcagcac
     8821 gcccttgcgc ttcagcggcg atggcttggc ctgctggggc ggcttcggcg gcgggatgaa
     8881 cgcggccgtc gggttgcgcg ggctgcgctg cggcggggcg ggcggcgcgg cggtggcggc
     8941 gccggtgcgc tgccacggcg gccgctcggc gggcgcgggg gcttcggcgg gcacggccgc
     9001 gggctggcgg ccggagctga acagcggcgg cgacaccatc gtgccgccct tggtgtcctt
     9061 ctcgccgttg gcgagcgcgg ccagccggtc gcgggcctcc agcatggtcg gccgctcctc
     9121 cggctcgacc cgcagcaggc tcatcagcag cgcggtcgcc ttgccggagc tgcgcggcgg
     9181 gatgacctgc ccgttcgccg ccgtgtagag cagggcgagc tggttggagc tgttgccgta
     9241 gggcggctgg ccctccagcg cgtggtagag cgtcgcgccg agggagaaga cgtcggaggc
     9301 cggcgccggg tccgagccgc gcgccagctc gggcgcgagg taggccgggg tgccgccgat
     9361 caggccggtc tgggtgagcg tgaggtcgcc tgccgcgcgc gagatgccga agtcggtgat
     9421 cttcgcggtg ccgttctcgt cgatcaggat gttcgccggc ttgatgtccc ggtgcacgat
     9481 cccggcgcgg tgcgccacga ccagcgccga ggcgacctgg tgcccgatgc gcgcgacctc
     9541 ggccagcggc agcgtgccct gctcggcgat caccgcggag aggctgcgcg agggcaggta
     9601 ctccatgacc aggcacggat cgcccccgtg ctcggcgatg tcgaacacca cgatggcgtt
     9661 cgggtgctgg aaccgcgccg cgttcttcgc ctcgcgcatc gctcgctggc gcatgctgtt
     9721 gcgctcggcc tcggacatgc ccggctgggc gaggatctgc ttcaccgcca ccgaacgctc
     9781 gaggcgctcg tcgaccgcgc gccacacgac gcccatggcg ccgctgccga tgtgctcaac
     9841 gaggcgatag tgccctgcga tcagctgacc ggtgtcgatg ggactactcc tcggaaactc
     9901 tccgcatgtg ctggcttggc tcagcgcgaa cgatctcctc gcgcaccggg tcgagtgtag
     9961 cgggctgcct gatacgcccg gcgttagctg tcccgcgggc gattcgcgcc agtccgacgg
    10021 cgccggcgac cgcgaaggca ccgtagcagg ccagcagcgc gggcggtgtg ctgccggtga
    10081 gcgcgtcgcc cagcaccacc accgcgatcg tgcccggcag gctgccgagg acggtcccga
    10141 tcaggtacgg gcggaaccgc accgagagga tgccgcagca ataacccagt ggtgcgaagg
    10201 gaaccagcgg gatcagccgc aatgaggcga ccgccaggat cccgccgtcg gcgagccgtt
    10261 cgtcgaccgc acgcacgact ttccgttcca ggtggcgggc cacgaggtcg cgcccgacca
    10321 gacgggccag gccgaacccg agcgcgccgg acaccgcggt cgccgtgatg gccaccacga
    10381 tcccgagcac gtcgccgagc agcaggccgg ccgcgaggtt gaagacggtg cgggggatgg
    10441 gggcgacggt gagcaccgcg tacgcggcca ggaacaccag tgcggtcacc gcgcccgcgc
    10501 cggcggccca gttgcgcagt tccgcggggc tgggcaccgg aagtgtgaac gccgccacga
    10561 ccgccccggc gagcaggacg agcgcgacga tcaacttcag gcggcgggac acgacgtcca
    10621 cggtaaggca cgcgcggaac cggcccggcg gggctacggt gatccggtgg caggacgcgg
    10681 tgaggcggtc gaataccagg tgggctcccg aacggtgcgg gtgtccaatc cggacaaggt
    10741 gtacttcccg gagcgcggga tcaccaagcg gcaggtcgtg gagtactacc tcgcggtcgc
    10801 ggagccgttg ctgcgggcgg tgggggagcg gccgacgacg ctgaaacggt tccccgacgg
    10861 cgtttccggt gagccgttct acgccaaacg ggttcccaag ggagcacccg tttgg
//
