LOCUS       NZ_BNAU01000005        21505 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Amycolatopsis deserti strain CGMCC 4.7677 sequence05, whole genome
            shotgun sequence.
ACCESSION   NZ_BNAU01000005
VERSION     NZ_BNAU01000005.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Amycolatopsis deserti
  ORGANISM  Amycolatopsis deserti
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 569082)
  AUTHORS   Sun,Q. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 82X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014653945.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:43:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,561
            CDSs (total)                      :: 7,498
            Genes (coding)                    :: 7,407
            CDSs (with protein)               :: 7,407
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 32 of 91
            Pseudo Genes (incomplete)         :: 64 of 91
            Pseudo Genes (internal stop)      :: 8 of 91
            Pseudo Genes (multiple problems)  :: 12 of 91
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:49:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 114842
            Orig. end                         :: 136347
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNAU01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21505
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ranthipeptide"
                     /region_number="1"
                     /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085
                     or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /tool="antismash"
     cand_cluster    1..21505
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /kind="single"
                     /product="ranthipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21505
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11505](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11505)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
     misc_feature    complement(35..50)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     misc_feature    complement(1061..1075)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.25"
                     /tool="antismash"
     misc_feature    complement(1490..1503)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.06"
                     /tool="antismash"
     misc_feature    complement(1864..1878)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.13"
                     /tool="antismash"
     gene            complement(1986..3101)
                     /locus_tag="IE290_RS21630"
                     /old_locus_tag="GCM10017786_43840"
     CDS             complement(1986..3101)
                     /EC_number="1.1.99.36"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-283). E-value: 8.3e-10.
                     Score: 30.2. Matches aSDomain:
                     nrpspksdomains_IE290_RS21630_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024538.1"
                     /locus_tag="IE290_RS21630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43840"
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="WP_191246502.1"
                     /sec_met_domain="ADH_N (E-value: 2.2e-29, bitscore: 96.6,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.3e-23, bitscore:
                     78.6, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKAAVLHSAGKPFEIEELELDGPREGEVLIKYTAAGLCHSDLH
                     LIDNDLVPRFPIVGGHEGAGIIEDVGPGVSKVEPGDHVVCSFIPNCGHCRYCATGRSNL
                     CDMGATILDGHMPDGSFRFHRGGTDYGAMCMLGTFSERATISEHSVVKVDDWLPLETAV
                     LVGCGVPTGWASANYAGGVRAGDTCVVYGIGGIGINAVQGAAHAGAANVIAVDPVAFKR
                     EKALELGATHAFASADEAAAKVAELTWGQMADQALITVGTVDEQVVTDAFNVVGKGGTV
                     VITGLANPEKLTVHLSGGVMTLFEKTVKGTLFGSANPQYDILRLLRLYQAGHVKLDELV
                     TKRYSLEEVNEGYQDLRDGKNIRGVIMHSAD"
     aSDomain        complement(2253..3044)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE290_RS21630_PKS_ER.1"
                     /evalue="8.30E-10"
                     /label="IE290_RS21630_PKS_ER.1"
                     /locus_tag="IE290_RS21630"
                     /protein_end="283"
                     /protein_start="19"
                     /score="30.2"
                     /tool="antismash"
                     /translation="LELDGPREGEVLIKYTAAGLCHSDLHLIDNDLVPRFPIVGGHEGA
                     GIIEDVGPGVSKVEPGDHVVCSFIPNCGHCRYCATGRSNLCDMGATILDGHMPDGSFRF
                     HRGGTDYGAMCMLGTFSERATISEHSVVKVDDWLPLETAVLVGCGVPTGWASANYAGGV
                     RAGDTCVVYGIGGIGINAVQGAAHAGAANVIAVDPVAFKREKALELGATHAFASADEAA
                     AKVAELTWGQMADQALITVGTVDEQVVTDAFNVVGKGGTVVI"
     misc_feature    3106..3121
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.96"
                     /tool="antismash"
     misc_feature    3116..3131
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.24"
                     /tool="antismash"
     gene            complement(3143..3820)
                     /gene="mftE"
                     /locus_tag="IE290_RS21635"
                     /old_locus_tag="GCM10017786_43850"
     CDS             complement(3143..3820)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421033.1"
                     /locus_tag="IE290_RS21635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43850"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_191246503.1"
                     /transl_table=11
                     /translation="MNLADLAWPELGDRRLLAVPLGATEQHGPHLPYTVDTEIAVELCR
                     RLGEARPEVVVAPALPYGSSGEHADFPGTLSIGQEATELLLVELVRSADAFAGVLLVSA
                     HGGNAVPLRRAVAKLRYEGRNVRAWCPDGPADDTHAGRLETSVMLALRPEAVRTNRFEP
                     GETRPLPELIGRLRDEGVRGVSPNGVLGDPARASAEAGRATLSRWADALLEAAAALARD
                     GIM"
     gene            complement(3817..5175)
                     /gene="mftF"
                     /locus_tag="IE290_RS21640"
                     /old_locus_tag="GCM10017786_43860"
     CDS             complement(3817..5175)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983859.1"
                     /locus_tag="IE290_RS21640"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; Members of this protein family, MftF,
                     are glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43860"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_191246504.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.4e-16,
                     bitscore: 55.2, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVLRLDPSTKAVAGGRVLVGGSPLRVVKLSAAGARLFAQWAAGA
                     AVGDKPHHRRLAEKLVLAGMVHPTYPGAKLTPQDVTVVVPVRDHADRLTGLLPRLNDVR
                     ETLVVDDGSAVPVPHAVDRHPVARGPAAARNTGWRKVTTELVAFLDADTRPEPGWLGPL
                     LAQFEDPAVVAVAPRVRSAPGRKALSRYETRQSSLDMGATPAQVRPRGRITYLPTAALV
                     VRTSALQAIHGFDEDLRFGEDVDLIWRLVDAGGSVRYEPESEVVHEPRSTWRAWLKQRY
                     SYGTSAAPLAKRHGRQALAPVRTAGWTALAWLALAAGRPKTGVAIALGTAALLPRKLKP
                     LDVPAKEALGIALRGHVAAGRFLADALTGAWGPAAIPLLALSRRGRRVLALAYARHLLD
                     WARTRPDIDPLRWLLARAADDLAYGAGVWRGCLREKEPGPLVPDLSTWPGKRL"
     gene            complement(5450..6235)
                     /locus_tag="IE290_RS21645"
                     /old_locus_tag="GCM10017786_43880"
     CDS             complement(5450..6235)
                     /NRPS_PKS="Domain: PKS_KR (4-178). E-value: 2.2e-09. Score:
                     29.4. Matches aSDomain:
                     nrpspksdomains_IE290_RS21645_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983860.1"
                     /locus_tag="IE290_RS21645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43880"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_191246505.1"
                     /transl_table=11
                     /translation="MTPRVAVVTGAARGIGAAVVHRLAGDGWRVVAVDVAEDLPGLRYP
                     LARREQLAEVAAKWPGQVIDLVGDVRDQRALDEAVRVAEREFGGLDAAVAAAAVMAGGR
                     PLWETTDSEWESLFGAGVDGVFHLARAAVPALLRRPRPRSGRFVALASAAGHTGLWRLA
                     GYNAAKHAVVGLIRGLATDLRGTGVTATAVSPGSTRTPMLAATAALYELDDVEEFAQHQ
                     LAERLLEPDEVAAAVCWLCGRDASAITGTVLHADGGFTA"
     aSDomain        complement(5702..6223)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE290_RS21645_PKS_KR.1"
                     /evalue="2.20E-09"
                     /label="IE290_RS21645_PKS_KR.1"
                     /locus_tag="IE290_RS21645"
                     /protein_end="178"
                     /protein_start="4"
                     /score="29.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAVVTGAARGIGAAVVHRLAGDGWRVVAVDVAEDLPGLRYPLARR
                     EQLAEVAAKWPGQVIDLVGDVRDQRALDEAVRVAEREFGGLDAAVAAAAVMAGGRPLWE
                     TTDSEWESLFGAGVDGVFHLARAAVPALLRRPRPRSGRFVALASAAGHTGLWRLAGYNA
                     AKHAVVGLIRG"
     CDS_motif       complement(6161..6217)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE290_RS21645_0001"
                     /evalue="1.40E-02"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE290_RS21645"
                     /protein_end="25"
                     /protein_start="6"
                     /score="8.1"
                     /tool="antismash"
                     /translation="VVTGAARGIGAAVVHRLAG"
     gene            complement(6274..8199)
                     /locus_tag="IE290_RS21650"
                     /old_locus_tag="GCM10017786_43890"
     CDS             complement(6274..8199)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983861.1"
                     /locus_tag="IE290_RS21650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43890"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 2"
                     /protein_id="WP_191246506.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.3e-10, bitscore:
                     36.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLLFEPLRLGPVEVRNRIVFAAHLTNYAAGNLPTEQHAAYYAER
                     AAGGAGLIISEEHVVHPSDWPYEKVIHGYRPDVVPGYRRITDAVHAHGVPIFAQLNHNG
                     GQASSMYTRRAVWAPSPVPDPLFREVPKAMSAAEIAEVVAGFGRVARHCAEGGFDGVEI
                     QCSQSSLVRGFLAPATNLRTDDYGGPLANRARILLEIIAAVRAAIGPQRALGVRLSGDD
                     LVEGGVTLDEAVEVARMIAPGIDYLNTSIGVATSTLYMVEPSMAVPRGYALPVSEALRR
                     AVPVPVVGIGRIKSPEEAERALAEGRCDLVGVVRGQIADPSFAAKARAGREVRTCLSCN
                     QECVGRMGLNRWLGCVENPRAGREAVPLPPPGPRPKRVLVVGAGPAGLKAAATAAERGH
                     HVTVLERGEPGGQIALAATAPGRAELGDLVRNLLTECRRAGVDMTTGCEATAAAVLAEA
                     PDAVVLATGARPVLPEWAVSPRVVHAREVLTGVSPWGEVVVIDEVGFHPATSVAELLAA
                     RGCRVTVSTPGMVVGQDLGLTLDRELFGRRAHGLGIAERTDEMVLAAAATEDGGVALRM
                     LRHTTGETREVRCDWVVCATHQEPEDTLWHALRDAPFQVLRVGDCVTPRRAHAAVLEGH
                     RAGVSL"
     gene            complement(8196..10004)
                     /locus_tag="IE290_RS21655"
                     /old_locus_tag="GCM10017786_43900"
     CDS             complement(8196..10004)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421037.1"
                     /locus_tag="IE290_RS21655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43900"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 1"
                     /protein_id="WP_191246507.1"
                     /transl_table=11
                     /translation="MTRLAGPVRVGGSTAPSRVLFGPHETNLARGRALAGRHVAYYRRR
                     AEGGAGIIVTETASVHPSDWPYERAPLAAECGPGWTSIVDACRPHGTLVVASLGHAGGQ
                     GSSAYHQSALWAPSRVPDPVSRELPMEMEGPEIAALIGGFRAAAALAAQSGVDGVEIDA
                     GQYSLLRQFCSGLTNRRTDAYGDRARLLREVIAAVREAIGDRILGLRLACDDGAPWGGI
                     TPETAPVPGGIDYLVPVRGSAFAGSRPDLRTEPGFNLDLCRAMSGHTMTVLQGSVVDPA
                     LAERALDFAGLVEMTRAQIADPDLVRHLRAGTDPRPCVLCNQKCRVRDPRNPIVSCIGE
                     PGSGHETEDPLPRAGSGEVLVVGGGPAGLEAARVLAVRGFDVELTERADRLGGMVRVAA
                     RLAGRDRLARLVDWLETEVRRLGVRISTGVETLAADGRPVIVATGSLPGPRDYVVDGGC
                     VLDVVSVLDGAALPDGPVVVHDPLGDWTGAGVAELLAAGRRVTIVSPDPVIATQVHDIA
                     AVNARLQRAGVALVKRSRPRVVRRDHVVVEDVLTGECRELPCAAVIHCGHRLPRPALPG
                     TAIGDAVAPRTIHEAVLDGRRAAVAL"
     gene            complement(10001..11200)
                     /gene="mftC"
                     /locus_tag="IE290_RS21660"
                     /old_locus_tag="GCM10017786_43910"
     CDS             complement(10001..11200)
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983863.1"
                     /locus_tag="IE290_RS21660"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="GCM10017786_43910"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_191246508.1"
                     /sec_met_domain="PTHR43273 (E-value: 4.9e-42, bitscore:
                     139.3, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 6.1e-25, bitscore: 83.6,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 9.8e-10, bitscore: 33.3,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 4.3e-15, bitscore:
                     51.0, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2.7e-06, bitscore: 22.9,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLVEHFKQGLNSPICLTWELTYACNLSCVHCLSSSGRRDPRELS
                     TAECKAVIDELQRMQVFYVNIGGGEPTVRSDFWELVEYAVDHQVGVKFSTNGVKLTPER
                     ARWLAATDYVDVQISLDGATAEVNDYVRGPGSYDTAIRAMRNLKDAGFRDFKLSVVMTR
                     HNVGQLDEFKAIADTYGAQLRITRLRPSGRGADTWDELHPTAEQQLQLYDWLIAHGEKV
                     LTGDSFFHLNALGSDPLPGLNLCGAGRVVCLIDPVGDVYACPFAIHEEFLAGNVRDEGG
                     FAGVWNTSELFERLRGPQTGGACTSCSAYDACQGGCMAAKFFTGLPLDGPDPECVKGHG
                     ELALAGVSAAPRSHIDHSHSKTRKSRKVAVSIGFGPPEGGARPDRACDTSPLAGFQPTR
                     "
     gene            complement(11221..11505)
                     /gene="mftB"
                     /locus_tag="IE290_RS21665"
                     /old_locus_tag="GCM10017786_43920"
     CDS             complement(11221..11505)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983864.1"
                     /locus_tag="IE290_RS21665"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="GCM10017786_43920"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_191246509.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 8.9e-31,
                     bitscore: 101.1, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLLTVVFDPDQAYRCSPRVALRPEPFGALAYDFGTRRLSFLKT
                     KILVEVVRTLGDQPDVHTTLDAAGVPDDQRPAYLKALAGLADNGTIEPR"
     aSDomain        complement(11233..11472)
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IE290_RS21665_Mycofactocin_RRE.1"
                     /evalue="4.50E-32"
                     /identifier="RREFam016.1"
                     /label="IE290_RS21665"
                     /locus_tag="IE290_RS21665"
                     /protein_end="91"
                     /protein_start="11"
                     /score="101.1"
                     /tool="antismash"
                     /translation="DQAYRCSPRVALRPEPFGALAYDFGTRRLSFLKTKILVEVVRTLG
                     DQPDVHTTLDAAGVPDDQRPAYLKALAGLADNGTI"
     gene            complement(11490..11594)
                     /gene="mftA"
                     /locus_tag="IE290_RS21670"
     CDS             complement(11490..11594)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983865.1"
                     /locus_tag="IE290_RS21670"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_191246510.1"
                     /transl_table=11
                     /translation="MSEAKTVAPAVEDTLVEEDLLVEEVSIDGMCGVY"
     gene            11679..12275
                     /gene="mftR"
                     /locus_tag="IE290_RS21675"
                     /old_locus_tag="GCM10017786_43940"
     CDS             11679..12275
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="mftR"
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421041.1"
                     /locus_tag="IE290_RS21675"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from the
                     terminal Val-Tyr dipeptide of the precursor peptide MftA,
                     through a radical SAM enzyme-mediated process; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="GCM10017786_43940"
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="WP_191246511.1"
                     /transl_table=11
                     /translation="MTEKAEPAPRAGRKPITSRAEVEHVAFELFATRGFDRTTIDDIAK
                     AAGIGRRTFFRYFPSKNDIAWGDFDGQLDKMRKRLASYPPDRPLPGVLREALVDFNRVD
                     PDEAVWHRQRMELILTTPALQAHSTLRYADWRQVIAEFVAERLGVAASALAPQTVAYTA
                     LGIALAAYEQWLRDPRADLADLMAAGVDQLTAALT"
     gene            complement(12280..12663)
                     /locus_tag="IE290_RS21680"
                     /old_locus_tag="GCM10017786_43950"
     CDS             complement(12280..12663)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163767.1"
                     /locus_tag="IE290_RS21680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43950"
                     /product="hypothetical protein"
                     /protein_id="WP_191246512.1"
                     /transl_table=11
                     /translation="MLRTSWTPGPADASGLVLVSVTDFTSDRLRDLPGIYRAGRQLSRL
                     WPTLDGAVGHWLWAEPSRRRTGSVSVWRDEDAMRGFVGLPVHRQIMRTYRGRGEIRSTT
                     WTATDPDPREVWRRASEFLRRPR"
     gene            complement(12668..13300)
                     /locus_tag="IE290_RS21685"
                     /old_locus_tag="GCM10017786_43960"
     CDS             complement(12668..13300)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF028235.5"
                     /locus_tag="IE290_RS21685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43960"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_191246513.1"
                     /transl_table=11
                     /translation="MQTDRRRERGQRTRETVLDAAVALASVHGLEGLTFGRLAEHLGTS
                     KSGLFAHWRDKEHLQLDAIDQARRQWTDRVMAPALRTPPGIRRVFALHEARMAFYADGV
                     LPGGCFFLAAQTEFDDRPGAVQAKTAQALHDWLAFIRSLVDEAIALGELTEDADPDQLA
                     YEIDALGESAVIHSRLVGHEVTFARARRAVLARLRALATDPATLPED"
     misc_feature    complement(13293..13308)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 18.61"
                     /tool="antismash"
     misc_feature    complement(13334..13350)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.58"
                     /tool="antismash"
     misc_feature    13338..13347
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    13338..13352
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.65"
                     /tool="antismash"
     gene            complement(13380..14249)
                     /locus_tag="IE290_RS21690"
                     /old_locus_tag="GCM10017786_43970"
     CDS             complement(13380..14249)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421042.1"
                     /locus_tag="IE290_RS21690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43970"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_191246514.1"
                     /transl_table=11
                     /translation="MTPDDGDGFVYCERGHRHWGLFGAAGLLLTDPERGVLLQHRAWWT
                     HHGETWALPGGARRGHESAREAATREAEEEAAVPVGAVRPTAESVADHGGWSYTTVLAN
                     TRHPVRERVVNEESAELRWVPPEEVGSFPLHDDFAAAWPALHAQLGRELVLVVDAANVV
                     GSRPDGWWRDRAGAAERLRDRLSGLVREGVRSADVGLGGAEWSWWPRVQMIVEGQARDV
                     PPADDVEVIAAPRDGDSQIVESVRKARDARPRDHVVVVTADRELRSRVEAEGANVLGPA
                     KLLNLVAD"
     gene            complement(14262..15197)
                     /locus_tag="IE290_RS21695"
                     /old_locus_tag="GCM10017786_43980"
     CDS             complement(14262..15197)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1108: ROK family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983868.1"
                     /locus_tag="IE290_RS21695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43980"
                     /product="ROK family protein"
                     /protein_id="WP_191246904.1"
                     /transl_table=11
                     /translation="MDVGGTSVRAGVVDEHGSILDTARAGTPSGEEALEDAITGVIDEL
                     RNRHEDVVGVGLAVAGFIANDRRTVMFAPHLAWRNAPVADRIAKRVGLPVTLEHDANAA
                     AVGEHRFGAARGAKVAALVAIGTGIGAGLLLDGELYRGANGVAPELGHLTVVPDGRACP
                     CGKYGCWERYCSGTALAATAIELLARYPGTSTILSRDIALDPGALTGRRVAGAARDGDP
                     IAQRAVAELAKWLGEGLALVADVFDPEIVVIAGGVSESAPLFLDEAREHYAAAITGGRH
                     RPLARIRTAQLGDDAALVGAAALALEARAV"
     gene            complement(15212..15532)
                     /locus_tag="IE290_RS21700"
                     /old_locus_tag="GCM10017786_43990"
     CDS             complement(15212..15532)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421044.1"
                     /locus_tag="IE290_RS21700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_43990"
                     /product="hypothetical protein"
                     /protein_id="WP_191246905.1"
                     /transl_table=11
                     /translation="MVVERAAPWLDGVVAAGHGTAESPEQGSECGWCPLCAIVAVVRGE
                     KPEFVARVLEQAAQIIALLRAVLADRWEPQEGVHMPGFQPPPRPRPESGRVQRIPVQWR
                     DG"
     misc_feature    15547..15561
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     gene            complement(15592..16734)
                     /locus_tag="IE290_RS21705"
                     /old_locus_tag="GCM10017786_44000"
     CDS             complement(15592..16734)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983870.1"
                     /locus_tag="IE290_RS21705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_44000"
                     /product="ArsA family ATPase"
                     /protein_id="WP_191246515.1"
                     /transl_table=11
                     /translation="MTRLLLFTGKGGVGKTTLAAATAAGLAARGYKTLVVSTDPAHSLG
                     DAFGRRLGREPSDVDTRLHGAQVDSRGLVDDIWQDLRGRLKGALAGAGVDALDAEELSV
                     LPGVDELLALTEVQRLAEAGRWDVVVVDCGPTAETLRLLALPEAVSGYLDKVFGWQLTL
                     TGSRGLARSVRDLAAHVSSLRDLLTDPAVTTVRLVLTPERVVVAEARRTLSSLALRGIR
                     VDGLIVNRLMPAPGVWRGAAANWMRTRRRQQDEVLAELTWELARVEHRAAEPVGLPALR
                     EIATELYGERDPLTGNSSESAPLLHVTEVDGGYRLRLALPLDRGAELDLARVDDDLAVT
                     VDGFRRLVALPEALRPCRITGAESDADGLVVSLTKPGEDS"
     gene            complement(16748..17191)
                     /locus_tag="IE290_RS21710"
                     /old_locus_tag="GCM10017786_44010"
     CDS             complement(16748..17191)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (3-144). E-value:
                     2.2e-21. Score: 68.7. Matches aSDomain:
                     nrpspksdomains_IE290_RS21710_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983871.1"
                     /locus_tag="IE290_RS21710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_44010"
                     /product="SRPBCC family protein"
                     /protein_id="WP_191246516.1"
                     /transl_table=11
                     /translation="MAEQSTQSIEVDAPPEKVMAVIADFASYPEWAKQVRETEVLATDD
                     TGRAKQVKLTLDAGPIKDVYTLEYDWAADGRSVSWHLVRGQMQKAQDGRYELVPVGEGR
                     TRVTYTLSVELVLPMIGLLRRKAEKMVMDTALKELKRRVEDLA"
     aSDomain        complement(16760..17182)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE290_RS21710_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="2.20E-21"
                     /label="IE290_RS21710_Polyketide_cyc2.1"
                     /locus_tag="IE290_RS21710"
                     /protein_end="144"
                     /protein_start="3"
                     /score="68.7"
                     /tool="antismash"
                     /translation="QSTQSIEVDAPPEKVMAVIADFASYPEWAKQVRETEVLATDDTGR
                     AKQVKLTLDAGPIKDVYTLEYDWAADGRSVSWHLVRGQMQKAQDGRYELVPVGEGRTRV
                     TYTLSVELVLPMIGLLRRKAEKMVMDTALKELKRRVE"
     gene            complement(17219..18010)
                     /locus_tag="IE290_RS21715"
                     /old_locus_tag="GCM10017786_44020"
     CDS             complement(17219..18010)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164579.1"
                     /locus_tag="IE290_RS21715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_44020"
                     /product="metallophosphoesterase"
                     /protein_id="WP_191246517.1"
                     /transl_table=11
                     /translation="MRVHVVSDVHGNTEALARAGEGADALIVLGDLLDFVDYHQHDQGI
                     LGTLFGPEKVATFARLRREGTREESIAFSKSLWSSLDDPAAAVEEAVREQYSAMFAAMT
                     APTYAIAGNVDSPDLWPEFAGDDVRLVEGEVVEIGGLRFGFAGGVVLPAGATIRRSNPV
                     WRPFLRTQEEFDEAVGKLTDIDVLCTHQPPALPELTYDVVARRHELGSSALRGLVYAEQ
                     PRWSLFGHVHQPLSARLRVGRTECRNVGHFKVTERPHVLRW"
     misc_feature    17995..18006
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            complement(18031..18432)
                     /locus_tag="IE290_RS21720"
                     /old_locus_tag="GCM10017786_44030"
     CDS             complement(18031..18432)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (14-90). E-value:
                     5.4e-07. Score: 22.1. Matches aSDomain:
                     nrpspksdomains_IE290_RS21720_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421047.1"
                     /locus_tag="IE290_RS21720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_44030"
                     /product="polyketide cyclase / dehydrase and lipid
                     transport"
                     /protein_id="WP_191246518.1"
                     /transl_table=11
                     /translation="MSTLNGAPPSIDIVDETFLAVPPGTVAAVFADPAAWRRYWPDLVL
                     EVYTDRGDEGLRWTVRGALVGTMEVWLERTLDGTLLHYFLRASPVAEMSPRRLRREFDR
                     RARAAKAVALGLKEILEDGREPGIPPRTS"
     aSDomain        complement(18163..18390)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE290_RS21720_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="5.40E-07"
                     /label="IE290_RS21720_Polyketide_cyc2.1"
                     /locus_tag="IE290_RS21720"
                     /protein_end="90"
                     /protein_start="14"
                     /score="22.1"
                     /tool="antismash"
                     /translation="DETFLAVPPGTVAAVFADPAAWRRYWPDLVLEVYTDRGDEGLRWT
                     VRGALVGTMEVWLERTLDGTLLHYFLRASPV"
     misc_feature    complement(18437..18449)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     misc_feature    18443..18460
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 17.72"
                     /tool="antismash"
     misc_feature    complement(18506..18521)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            18546..20342
                     /locus_tag="IE290_RS21725"
                     /old_locus_tag="GCM10017786_44040"
     CDS             18546..20342
                     /NRPS_PKS="Domain: AMP-binding (22-466). E-value: 3.4e-97.
                     Score: 317.6. Matches aSDomain:
                     nrpspksdomains_IE290_RS21725_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074387.1"
                     /locus_tag="IE290_RS21725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_44040"
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="WP_191246519.1"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-93, bitscore:
                     309.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREYSAPAVQPVADDENLADIVWANAERFPDAISFRRQVDGSWLD
                     VTAREFAAQVIGVAKGLVEAGIERGDRVGLMSKTRYEWTLIDFAIWAAGAVTVPIYDTS
                     SPEQVAWILSDSGAKGVFVETGEHLAAVDEVRDRLPALRYTWQIEAARPAVDELTTLGA
                     DVADDTVHERRRLVRAGDLATIVYTSGTTGRPKGVELTHRNLLAEIRADINAFPQLMEQ
                     GNSLLCFLPLAHVLARAIAVTAVTARVTLGHTPDVKNLVADLGTFRPTFVVAVPRVFEK
                     VYNGAKQKAHSEGKGKIFDAAEQVAVSYSEARDSGGAGLALKAQHFLFDKLVYGKLRAA
                     LGGRCIAAVSGGAPLGPRLAHFFRGIGVPVFEGYGLTETSAAANVNTEHAFRVGTVGRP
                     VAGTSVRIADDGEVLLKGDVVFGAYFNNETATKEALEDGWFHTGDLGELDDDGFLKITG
                     RKKEIIVTAGGKNVAPSGLEDTMRAHPLISQAMVVGDQRPFIGALITIDEEFFPAWKSQ
                     NGKPADATVADLAQDPDLIAAVQGAVDEANKQVSHAEAIKKFVILGNDFTEAGGEITPS
                     LKLKRNVVTKNYATSIEALYQK"
     aSDomain        18612..19943
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE290_RS21725_AMP-binding.1"
                     /evalue="3.40E-97"
                     /label="IE290_RS21725_AMP-binding.1"
                     /locus_tag="IE290_RS21725"
                     /protein_end="466"
                     /protein_start="22"
                     /score="317.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="WANAERFPDAISFRRQVDGSWLDVTAREFAAQVIGVAKGLVEAGI
                     ERGDRVGLMSKTRYEWTLIDFAIWAAGAVTVPIYDTSSPEQVAWILSDSGAKGVFVETG
                     EHLAAVDEVRDRLPALRYTWQIEAARPAVDELTTLGADVADDTVHERRRLVRAGDLATI
                     VYTSGTTGRPKGVELTHRNLLAEIRADINAFPQLMEQGNSLLCFLPLAHVLARAIAVTA
                     VTARVTLGHTPDVKNLVADLGTFRPTFVVAVPRVFEKVYNGAKQKAHSEGKGKIFDAAE
                     QVAVSYSEARDSGGAGLALKAQHFLFDKLVYGKLRAALGGRCIAAVSGGAPLGPRLAHF
                     FRGIGVPVFEGYGLTETSAAANVNTEHAFRVGTVGRPVAGTSVRIADDGEVLLKGDVVF
                     GAYFNNETATKEALEDGWFHTGDLGELDDDGFLKITGRKKEIIVT"
     CDS_motif       19092..19151
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE290_RS21725_0001"
                     /evalue="5.50E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="IE290_RS21725"
                     /protein_end="202"
                     /protein_start="182"
                     /score="25.1"
                     /tool="antismash"
                     /translation="ATIVYTSGTTGRPKGVELTH"
     gene            20364..21140
                     /locus_tag="IE290_RS21730"
                     /old_locus_tag="GCM10017786_44050"
     CDS             20364..21140
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421049.1"
                     /locus_tag="IE290_RS21730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_44050"
                     /product="MEDS domain-containing protein"
                     /protein_id="WP_229874645.1"
                     /transl_table=11
                     /translation="MLHDHRCWAFESPDEFQARAREFLRDGLARGTQVWFVTPGEPVLD
                     GLDEALDTGAARVLSLDETYSVGTVVDPDAQARSYAEATEAALAAGYTGLRVAADHTAL
                     VATPEQLDAFARYEHTVDRYAAAGTFSAMCGYDLGRVDDEVVARVACLHPVGNADPGFR
                     LFGSASASAALAGELDLYTEDLLTEALRRADPRPVDGRLVLDVSGLRFADHRSLTRLAE
                     HAEGEGATLVLRDPSPAVTRLVEVLGLPSVRVEGVA"
     misc_feature    21060..21062
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(21387..21401)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.98"
                     /tool="antismash"
ORIGIN
        1 gaggatctcg ccgaggccgt ggccgaagtc ggtggtgacg ctggtgtgcc gctgctcgta
       61 gctgacgacg ttgcccagcg cgtggttgac ccgcacccgc gagatgcaca tcgggtgcgt
      121 gcggccgtgg cgggcgtcgt cggcgcggtg ccacatgagc ttgacgggct tgcccatctt
      181 cttcgagatc tccgccgcct cgatcgcggc gtcgccgaag agcttgcggc cgaacgaacc
      241 gccaccggtg accacgtgca ccttgaccgc cgggatcggc aggccgagct tgagcgcgat
      301 ctgctcctgc gtggtgatcg gcgacttgag gccggaccag atctccgcgc ggtcggcgcg
      361 cacgtcggcg atggcgcagt tggtctccag cgcgctgttg ctggcgaagg cgaacacgaa
      421 ctcggcgtcg atcgactggg tcaggaccgg gggcaccgcc atcggcagcg cggcggcgcg
      481 cagcttctgc tcgaccgagg cgtcggactc accgtccaca gtgcccggtc cccagtccac
      541 cttcagcgcc cggatcgcgt cgatgcactg cccgaaggtc tcggcgcgga ccgcgacgcc
      601 ggtggagatg gtggcgacgt cggtgacgcc gggcatcgcc ttgacctcgg ccaggttgtg
      661 gaccgccttg accgtgccgt tgatcgtcgg cgggcggcag accatcgtcg gcttggcgtt
      721 cgggacctgc aggtccatgg tgaacttctt gcgcccggtg accgcttcga gggcgtcgat
      781 gcggccggtc ggcttgccga tgatgcggaa ctcggaggtc ttcttcagcg tggcgctggt
      841 ttcctcggtg cgggcgctcg cggcggcttc ggcgagcgag ccgtaggtcg cggtgcggcc
      901 ggaggcggag cggatcacgc cgtcgcgggc ggtgagcgtc gaggcgtcga ccttccactg
      961 gtcggcggcg gtggcgagca ggcgggcgcg ggcgatggcc gcggcggtgc ggaccgggtt
     1021 gtacagcgag cgcaccgagt tggacccacc ggtcagctgg ttgaacacca gttccgggcg
     1081 ggcgtcggcg aggttgacgc cgaccttgct cagcgggagg tccatctcct cggcgatgag
     1141 catggcgatg gtggtggtga tgccctggcc gacctcggcg cgcggcaccg cgaacgacac
     1201 cgtgtcgtcg cggttgacct ggatcgcgat gaggttggag gtcggcatcg ccgcgagcgt
     1261 gagggcgtcg ccgaggtcga ggatgtcggc cggcccgggg agcgtgggga tcgcggcgtc
     1321 ggccggagcc ggggcggcga ggtccccgcc gagcttggcc gccaccgcga gggtgggcgc
     1381 cgcgaggagg taaccgagga accggcgccg ttccagagac ggagtcccgt ccccgggcgc
     1441 ggccgatcgc cgttgatgcg ccatcgagag tgtccttccg agatccacgt tgatcgcggt
     1501 cacgatagga acgggctccg cgggggtcaa cgatccgtag ccgcgtcggc ccatctctgt
     1561 gaccggaaca cagtcgcgtg ccacacaact gtgacgcgga cacagccgaa cgtcgtacta
     1621 acctggtccg gatggtctac cgagagcgac ggtcgatcaa tcgagccggt ggcaggttcg
     1681 tgccgtggcg gcgagcatgg ttcagcccgg caacgcctca ctggtcgcct gccgtccgcg
     1741 accgagatcg ccatcccggt gcgggtgcac gaggtgaaga cggggcggcc cgtcaccggc
     1801 acgcgcgtgg agatcggcgg cgcctcctgc ccgccggtgc tgcactacac ccccacatcg
     1861 acctggccgt ggacatcccg ccgagccagg tgcacgtgac gccctcggac gccgacgtgc
     1921 gtgcggcgtt ccagccgctg gtcgacccgt gagggcggga agccccgccc tcacgggggc
     1981 acgactcagt ccgccgagtg catgatcacg ccacggatgt tcttcccgtc gcgcaggtcc
     2041 tggtagccct cgttgacctc ttcgagcgag taccgcttgg tcaccagctc gtccagcttc
     2101 acgtgaccgg cctggtacag gcgcagcagg cggaggatgt cgtactgcgg gttggccgag
     2161 ccgaacagcg tgcccttgac cgtcttctcg aacagggtca tcacgccgcc ggacaggtgc
     2221 accgtcagct tctccgggtt ggccaggccg gtgatcacga ccgtgccgcc cttgccgacc
     2281 acgttgaacg cgtccgtcac gacctgctcg tccaccgtgc cgacggtgat cagcgcctgg
     2341 tcggccatct ggccccaggt cagctccgcg accttcgccg cggcctcgtc cgccgaggcg
     2401 aacgcgtggg tggcgccgag ttcgagagcc ttctcgcgct tgaaggccac cgggtcgacg
     2461 gcgatcacgt tcgccgcgcc cgcgtgcgcc gcgccctgga cggcgttgat gccgatcccg
     2521 ccgatgccgt acacgacgca ggtgtcgccc gcgcgcacgc cgccggcgta gttggccgag
     2581 gcccagccgg tcggcacacc gcagcccacc agcaccgccg tctccagcgg cagccagtcg
     2641 tccaccttga ccaccgagtg ctcggagatc gtggcgcgct cggagaacgt gccgagcatg
     2701 cacatggcgc cgtagtcggt gccgccgcgg tggaagcgga agctgccgtc cggcatgtgc
     2761 ccgtcgagga tcgtcgcgcc catgtcgcac aggttcgagc ggccggtggc gcagtagcgg
     2821 cagtggccgc agttcgggat gaacgagcag acgacgtggt cgcccggctc gaccttggag
     2881 acgcccgggc cgacgtcttc gatgatgccc gcgccctcgt gcccgcccac gatcgggaac
     2941 cgcggcacca ggtcgttgtc gatcaggtgc aggtccgagt ggcacagccc ggcggccgtg
     3001 tacttgatca ggacctcacc ttcgcgtgga ccgtcgagct cgagttcctc gatctcgaac
     3061 ggcttgcccg cggaatgcag gactgcggcc ttggtcttca caccgatcac ctctttgtga
     3121 cagggctctc atgcaccgac tgctacatga tgccatctct ggcaagcgcc gcggccgctt
     3181 caagtagagc gtccgcccat cgactgaggg tggcgcgccc cgcttccgcg ctcgcccgcg
     3241 cggggtcacc cagcaccccg ttcgggctca ccccgcgcac accttcgtca cgcaaccgtc
     3301 cgatgagttc cggcagcggc cgcgtctcgc cgggctcgaa ccggttcgtg cgcacggctt
     3361 ccggccgcag cgcgagcatc accgacgtct ccagccgccc ggcgtgggtg tcgtcggccg
     3421 ggccgtccgg gcaccacgca cgcacattcc ggccctcgta acgcagtttc gcgaccgcgc
     3481 ggcgcagcgg gaccgcgttg ccgccgtgcg cggacaccag cagaaccccg gcgaaggcgt
     3541 ccgcggagcg caccagctcg accaggagga gttccgtggc ttcctgaccg atcgagagcg
     3601 tgccaggaaa atcggcgtgc tccccgctgg acccgtaggg cagcgcgggg gcgacgacga
     3661 cctccggccg ggcctcgccg agccggcggc acagctcgac cgcgatctcg gtgtccaccg
     3721 tgtacggcag gtgcggtccg tgctgttcgg tggcgcccag cggcacggcc agcaggcggc
     3781 ggtcgccgag ctcgggccag gcgaggtcgg cgaggttcac agccgtttcc ctggccacgt
     3841 ggagaggtcc ggaaccaacg ggcccggttc cttctcgcgc aggcagccgc gccagacacc
     3901 ggcgccgtag gcgagatcgt ccgcggcgcg ggccagcagc caccggagcg ggtcgatgtc
     3961 cgggcgggtg cgggcccagt ccaggaggtg ccgggcgtag gccagggcca agacgcgacg
     4021 gccgcgccgg gacagcgcga gcaacgggat cgcggccggg ccccacgcgc cggtgagggc
     4081 gtcggccagg aaccggcccg cggcgacgtg cccgcgcagg gcgatgccga gcgcttcctt
     4141 cgccggcacg tcgaggggtt tcagcttgcg gggcaacagg gccgcggtgc cgagcgcgat
     4201 cgcgacgccg gtcttcgggc gtccggcggc cagcgcgagc caggccagtg cggtccagcc
     4261 ggccgtgcgg accggcgcga gcgcctgccg cccgtggcgt ttcgcgagtg gcgcggcgga
     4321 agtgccgtag ctgtaccgct gtttcagcca ggcgcgccag gtcgagcgtg gttcgtgcac
     4381 cacttcggac tccggctcgt agcggacgga gccgccggcg tcgacgaggc gccagatcag
     4441 gtcgacatcc tcgccgaagc gcaggtcctc gtcgaagccg tggatcgcct gcagcgccga
     4501 ggtgcgcacg acgagcgcgg cggtgggcag gtaggtgatg cggccgcggg ggcggacctg
     4561 cgccggggtg gcgcccatgt ccaacgagga ctgtcgagtc tcgtagcggg acagggcttt
     4621 gcggcctggg gcgctgcgca cccgcggcgc gacggccacc acggcggggt cctcgaactg
     4681 cgccagcagc ggcccgagcc agccgggttc ggggcgggtg tcggcgtcga ggaaggcgac
     4741 cagctccgtg gtgaccttgc gccagccggt gttgcgtgcg gcggcggggc cacgcgcgac
     4801 ggggtggcgg tcgacggcgt gcgggacggg cacggcggac ccgtcgtcga cgacgagcgt
     4861 ctcgcggacg tcgttcaggc gcgggaggag cccggtgagg cggtcggcgt ggtcgcggac
     4921 ggggacgacg acggtgacgt cctgcggggt cagcttcgcg ccggggtagg tggggtggac
     4981 catgccggcg aggacgagtt tctcggccag gcgccggtgg tggggcttgt cgccgaccgc
     5041 cgcgccggcg gcccattggg cgaacaggcg cgccccggcg gcggagagct tgacgacccg
     5101 gaggggcgag ccgccgacga gaacccgccc gccggcgacg gccttggtgg agggatccag
     5161 gcggagcacg gtcatggcgt gacctcccgg tggcacccgc tcgcgcgcca gcgccggtgg
     5221 tacccgcggt cggccgtggc cccgacggcc cacgccgcca ggcgcaccgc accgcgagcg
     5281 cgcccgtcgc gagcagccgt ggccgcggag ccgagccgag gcgtgccgtg cgcgcgcacc
     5341 acgccgccac cgcaccacca ccggcggccg ctgccggctg tcgcccggat ggcgcctgcc
     5401 tcggggcgca ccgcaccgcg agcagccgtg gccgggaact ccagcggagt catgccgtga
     5461 aacccccgtc cgcgtggagc accgtgccgg tgatggcgct cgcgtcgcgg ccgcacagcc
     5521 agcacaccgc cgcggccacc tcgtcgggct ccagcagccg ctcggcgagc tggtgctggg
     5581 cgaactcctc gacgtcgtcc agttcgtaca gcgcggccgt ggccgccagc atcggcgtgc
     5641 gggtcgagcc cggcgagacg gccgtcgcgg tgacgcctgt gccgcgcagg tccgtggcga
     5701 ggccgcggat cagcccgacg accgcgtgct tggccgcgtt gtagccggcc agccgccaca
     5761 gcccggtgtg cccggcggcc gaggcgagcg cgacgaagcg cccactgcgc ggccgcgggc
     5821 gccgcagcag ggccggcacc gcggcgcggg ccaggtggaa cacaccgtcc accccggcgc
     5881 cgaacagcga ttcccattcg gaatcggtgg tctcccacag tgggcgccca ccggccatga
     5941 ccgccgcggc ggccaccgcc gcgtccagcc cgccgaactc ccgctcggcg acgcggacgg
     6001 cctcgtcgag ggcccgctgg tcccgcacat cgccgacgag gtcgatcacc tggcccggcc
     6061 acttcgcggc gacctcggcg agctgctccc gccgcgcgag ggggtaccga agccccggca
     6121 ggtcctcggc gacgtccacg gcgaccacgc gccagccgtc cccggccagc cggtgcacca
     6181 cggccgcccc gatcccccga gcagccccag tcaccacggc gacgcgcggg gtcacgtgat
     6241 catctccacg cggggagcct gccacttccc cgctcacagc gacacccccg cccgatggcc
     6301 ctccagcacc gccgcgtgcg cgcgccgcgg cgtgacgcag tcccccaccc gcaacacctg
     6361 gaacggcgcg tcccgcaggg cgtgccacag cgtgtcctcc ggttcctggt gcgtcgcgca
     6421 caccacccag tcgcagcgga cctccctcgt ctcgccggtc gtgtgcctca acatccgcag
     6481 cgccacgccc ccatcctccg tcgccgcggc ggcgagcacc atctcgtccg tgcgctccgc
     6541 gattcccagc ccgtgcgcgc gccggccgaa cagctcccgg tccagcgtta gcccgaggtc
     6601 ctgcccgacg accatccccg gcgtcgacac cgtcacccgg cacccgcgcg cggccagcag
     6661 ctcggccacc gacgtcgccg ggtggaagcc cacctcgtcg atcacgacca cctcacccca
     6721 gggcgaaacc ccggtcagca cctcccgcgc gtgcaccacc cgcggcgaaa ccgcccactc
     6781 cggcagcacc ggccgggccc ccgtcgccag caccacggcg tccggcgcct ccgccagcac
     6841 cgcagcggcc gtcgcctcgc agccggtcgt catgtcgaca cccgcacgcc ggcactcggt
     6901 cagcaggttg cgcaccaggt cccccagctc ggcgcgcccg ggcgcggtcg cggccagcgc
     6961 gatctgcccg cccggctcac cgcgctccag caccgtcacg tggtgtccgc gctccgccgc
     7021 cgtcgccgcc gccttcagcc ccgccgggcc ggcgccgacc accagcaccc gcttgggacg
     7081 cggccccggc ggcggcaacg gcaccgcctc ccgcccggcg cgcgggttct ccacgcaccc
     7141 cagccaccgg ttgagcccca tccgccccac gcactcctgg ttgcaggaca ggcacgtccg
     7201 cacctcccgc ccggctcgcg ccttcgccgc gaacgacggg tcggcgatct gcccgcgcac
     7261 caccccgacc aggtcgcacc gcccctccgc cagcgcgcgc tcggcctcct cgggcgactt
     7321 gatccgcccg atcccgacca ccggcaccgg caccgcccgc cgcaacgcct cggacaccgg
     7381 caacgcgtac ccccgcggca ccgccatcga cggctcgacc atgtacaacg tcgaggtcgc
     7441 gaccccgatc gacgtgttca ggtagtcgat gccgggcgcg atcatccgcg ccacctcgac
     7501 cgcctcgtcg agcgtgacgc cgccctcgac caggtcgtcg ccggacagcc gcaccccgag
     7561 cgcgcgctgc gggccgatcg ccgcacgcac cgccgcgatg atctcgagca ggatccgggc
     7621 ccggttcgcc agcggcccgc cgtagtcgtc ggtgcgcagg ttcgtcgcgg gcgccaggaa
     7681 cccgcgcacc agcgacgact gcgagcactg gatctccacg ccgtcgaacc cgccctcggc
     7741 gcagtgccgc gcgacccgcc cgaacccggc gaccacctcg gcgatctccg cggcggacat
     7801 cgccttgggc acctcgcgga acagcggatc cggcaccggc gagggcgccc acaccgcccg
     7861 ccgcgtgtac atcgacgacg cctgcccgcc gttgtggttg agctgcgcga agatcggcac
     7921 cccgtgcgcg tgcaccgcgt cggtgatccg gcggtacccc ggcacgacgt ccggccggta
     7981 cccgtggatc accttctcgt agggccagtc cgaggggtgc accacgtgct cctcggagat
     8041 gatcagcccg gcgccgcccg cggcccgctc ggcgtagtag gccgcgtgct gctcggtggg
     8101 caggttgccg gcggcgtagt tggtcaggtg cgcggcgaac acgatccggt tgcgcacctc
     8161 caccggcccc agccgcaacg gttcgaacag caggctcaca gcgccaccgc cgcgcggcga
     8221 ccgtccaaaa cagcctcgtg gatggtccgg ggcgccaccg catcaccgat cgccgtcccc
     8281 ggcaacgcag gtcgcggaag tcggtgtcca caatggatca ccgctgcgca cggcagctcc
     8341 cggcactccc cggtgagcac gtcctccacc accacgtgat cccgccgcac cacgcgagga
     8401 cgcgaccgct tgacgagcgc gaccccggcc cgctgcaggc gcgcgttcac cgcggcgatg
     8461 tcgtgcacct gcgtcgcgat caccggatcc gggctgacga tcgtcacccg acgcccggcg
     8521 gccagcagct ccgcgacccc cgcgccggtc cagtcgccga gcggatcgtg caccaccacc
     8581 ggcccgtccg gcagcgccgc accgtccaac acggaaacca catccagcac acaaccgccg
     8641 tcgacgacgt agtcacgcgg accgggcagg ctgccggtcg ccacgatcac cggccgcccg
     8701 tcggccgcca gagtctccac gccggtcgag atccgcacac cgagccgccg gacctcggtc
     8761 tccagccagt ccaccagccg cgccagccgg tcccgcccgg cgagccgcgc cgcgacgcgg
     8821 accatcccgc cgagccggtc ggcccgctcg gtcaactcga cgtcgaaccc ccgcaccgcg
     8881 agcacccgtg cggcctccag cccggccggc ccgccaccga ccaccagcac ctcaccggaa
     8941 ccggcacgcg gcagcggatc ctcggtctcg tgcccgctgc ccggctcgcc gatgcagctc
     9001 acgatcgggt tgcgtggatc ccgcacgcgg cacttctggt tgcacagcac gcacggccgc
     9061 ggatccgtcc ccgcccgcag gtgccggacc aggtccgggt cggcgatctg cgcgcgggtc
     9121 atctcgacca gcccggcgaa gtccagcgcc cgctccgcga gtgccggatc gaccacgctg
     9181 ccctggagca cagtcatcgt gtgccccgac atcgcccggc acaggtcgag gttgaacccc
     9241 ggttccgtgc gcagatcggg ccgggacccg gcgaacgccg aaccccgcac cggcaccagg
     9301 tagtcgatcc cgccgggcac gggtgcggtc tccggcgtga tcccgcccca cggcgcgcca
     9361 tcgtcgcacg ccaggcgcag gccgaggatg cggtccccga tcgcctcccg gaccgccgcg
     9421 atcacctcac gcagcaaccg cgcccggtca ccgtaggcgt cggtgcgccg gttggtcagc
     9481 ccggaacaga actgccgcag cagcgagtac tggccggcgt cgatctccac accgtccaca
     9541 ccggactgcg cagccagtgc cgccgcggcg cggaacccgc cgatcagcgc cgcgatctcc
     9601 gggccttcca tctccatcgg cagctcacgg ctcaccggat ccggaacccg agacggcgcc
     9661 cacaacgccg actggtggta ggccgacgag ccctgcccac cggcatgacc gagactcgcc
     9721 accacgagcg tcccgtgtgg acgacaggcg tccacgatgg acgtccagcc gggaccgcac
     9781 tccgcggcca gcggcgcgcg ttcgtacggc cagtcggagg ggtgcacgct cgccgtctcg
     9841 gtgacgatga tcccggcgcc gccctcggcc cgccgccggt agtaggcgac gtgccggccg
     9901 gcgagcgccc ggccccgagc caggttggtt tcgtgcggac cgaagaggac ccggctcggg
     9961 gcggtgctgc cgccgacccg gacggggccg gcgagcctgg tcatcgcgtg ggctggaacc
    10021 ccgcgagcgg gctggtgtcg cacgcccggt ccggccgggc gccgccctcg ggcgggccga
    10081 aaccgatgct gaccgccacc ttgcgactct tgcgggtctt cgaatgcgag tggtcgatgt
    10141 gcgagcgcgg cgccgcgctg accccggcca gcgccagttc accgtgcccc ttcacgcatt
    10201 ccgggtccgg gccgtccagc ggcagtccgg tgaagaactt cgccgccatg cacccgccct
    10261 ggcaggcgtc gtaggccgaa cacgacgtgc acgcgccccc ggtctgcggg ccacgcaacc
    10321 gctcgaacag ctccgaggtg ttccacacgc cggcgaaccc gccctcgtcg cggacgttgc
    10381 cggcgaggaa ctcctcgtgg atcgcgaacg ggcaggcgta gacgtcgccg accgggtcga
    10441 tcaggcacac cacccggccc gcgccgcaca ggttcaggcc cggcagcggg tccgagccga
    10501 gcgcgttgag gtggaagaac gagtcaccgg tgaggacctt ctcgccgtgg gcgatcagcc
    10561 agtcgtagag ctgcaactgc tgctcggcgg tcgggtgcag ctcgtcccag gtgtccgcgc
    10621 cgcggccgga cgggcgcagc cgcgtgatgc gcagctgggc gccgtaggtg tcggcgatgg
    10681 ccttgaactc gtcgagctga ccgacgttgt gccgggtcat cacgacggaa agcttgaagt
    10741 cgcggaaccc ggcgtccttc agattccgca tcgcccggat cgcggtgtcg taggaacccg
    10801 gaccgcggac gtagtcgttg acctcggcgg tcgcgccgtc cagcgagatc tgcacgtcca
    10861 cgtagtcggt cgcggcgagc caccgcgccc gctccggggt gagcttcacg ccgttggtgg
    10921 agaacttgac gcccacctgg tggtccacgg cgtattcgac cagttcccag aagtcggagc
    10981 gcacggtcgg ctcgccgccg ccgatgttga cgtagaagac ctgcatgcgc tgcagctcgt
    11041 cgatgacggc cttgcactcc gccgtgctca gctcccgcgg gtcgcggcgg cccgacgagg
    11101 acaggcagtg cacgcacgag aggttgcagg cgtaggtcag ttcccaggtc aggcagatgg
    11161 gcgagttcag cccctgcttg aagtgttcga cgagcttcat cgcacgccct tctcagtggt
    11221 tcagcgcggt tcgatcgtgc cgttgtcggc cagtcccgcc agcgccttca ggtaggccgg
    11281 gcgctggtcg tccgggacgc cggcggcgtc gagggtggtg tgcacgtcgg gctggtcccc
    11341 gagcgtgcgc accacctcga ccaggatctt cgtcttcagg aaggacagcc tccgggtgcc
    11401 gaagtcgtag gcgagcgccc cgaacggttc cggtcgcaac gcgacgcgcg gggagcaccg
    11461 gtaggcctgg tccggatcga acacgacggt cagtagacgc cgcacatgcc gtcgatggag
    11521 acctcttcga cgagcaggtc ctcctcgacg agcgtgtctt ccacggcggg ggcgacggtc
    11581 ttggcctcgg acatgggatg ctcccttcgg cgatgggccg ctccggtgcg gccgcgcttc
    11641 tcggacgata atggcaccga gtgccgaatg gaagagggat gaccgaaaag gcggagccgg
    11701 cgcccagggc ggggcgcaag ccgatcacgt cgcgggcgga ggtcgagcac gtggcgttcg
    11761 aactgttcgc cacccgcggc ttcgaccgca cgacgatcga cgacatcgcg aaggccgccg
    11821 gcatcgggcg gcgcacgttc ttccgctact tcccgtcgaa gaacgacatc gcctggggcg
    11881 acttcgacgg gcagctggac aagatgcgca agcggctcgc ctcctacccg ccggatcgcc
    11941 cgctgcccgg cgtgctgcgc gaggcgctgg tggacttcaa ccgggtcgac ccggacgagg
    12001 cggtgtggca ccggcagcgg atggagctga tcctgaccac cccggcgctg caggcgcatt
    12061 cgacgctgcg ctacgcggac tggcggcagg tgatcgcgga gttcgtcgcg gaacggctcg
    12121 gggtggccgc ctcggcgctc gccccgcaga ccgtggccta caccgcgctg ggcatcgcgc
    12181 tggcggccta cgagcagtgg ctgcgcgacc cgcgcgcgga cctcgccgac ctgatggccg
    12241 ccggggtgga ccagctgacc gcggcgctga cctgagccgt cagcggggcc ggcggaggaa
    12301 ctccgacgcc cggcgccaca cctcgcgcgg gtccggatcg gtcgcggtcc acgtggtgga
    12361 gcggatctcg ccgcgcccgc ggtaggtccg catgatctgc cggtgcacgg gcaggccgac
    12421 gaagccgcgc atggcgtcct cgtcgcgcca caccgagacc gagccggttc tgcgccgcga
    12481 gggctcggcc cagagccagt gcccgaccgc gccgtcgagg gtcggccaca ggcgggacag
    12541 ctgcctgccc gcgcggtaga tccccggcag gtcgcgcaac cggtcggagg tgaagtcggt
    12601 gacgctgacg agcacgagcc cgctcgcgtc ggcgggtccg ggtgtccacg aggtgcgcag
    12661 caaggtttca gtcctccggc agggtggcgg gatcggtggc gagggcgcgc agccgcgcca
    12721 ggacggcgcg gcgggcgcgg gcgaaggtga cctcgtgccc gaccagccgc gagtggatca
    12781 cggccgactc gccgagcgcg tcgatctcgt aggcgagctg gtccgggtcg gcgtcttcgg
    12841 tgagctcgcc cagcgcgatc gcctcgtcga cgagcgagcg gatgaaggcg agccagtcgt
    12901 gcagcgcctg cgccgttttg gcctggacgg cgccgggccg gtcgtcgaac tccgtttgcg
    12961 cggcgaggaa gaagcaccca ccgggcagga caccgtcggc gtagaaggcc atccgagcct
    13021 cgtgcagcgc gaacacccgc cggatgccgg gtggcgtgcg cagcgcgggc gccatgacgc
    13081 ggtccgtcca ctgcctgcgc gcctggtcga tcgcgtcgag ctgcaggtgc tccttgtccc
    13141 tccagtgcgc gaacagcccg gacttgctgg tgccgagatg ctcggcgagc cgcccgaacg
    13201 tgagaccttc cagcccgtgc accgaggcca gcgccaccgc cgcgtcgagg acggtttccc
    13261 gcgtgcgctg accgcgctcg cgccgccgat cggtttgcat gggtcgaccg tacactagaa
    13321 gaccgatcgg tcggtcttat tttttgacca tgcctgccca gtcgcggttc cgccgggtgc
    13381 tagtccgcca ccaggttcag cagcttcgcc gggcccagca cgttcgcgcc ctccgcctcg
    13441 actctggagc gcagctcgcg gtcggccgtc accaccacca cgtggtcccg cggccgggcg
    13501 tcccgcgcct tgcggacgct ctccacgatc tgcgaatcac cgtcgcgggg cgcggcgatc
    13561 acctcgacgt cgtcggccgg gggcacgtcc cgcgcctggc cctcgacgat catctgcacc
    13621 cgcggccacc acgaccactc cgccccgccg agccccacgt ccgcgctgcg cacaccctcg
    13681 cggaccagcc cggacagccg gtcccgcaac cgctccgccg caccggcccg gtcccgccac
    13741 cagccgtccg gccgggaccc gaccacgttg gccgcgtcga ccaccagcac cagttcgcgc
    13801 cccagctgcg cgtgcagcgc cggccacgcg gcggcgaagt cgtcgtgcag cgggaacgac
    13861 ccgacctcct ccggcggcac ccaccgcagc tcggcgctct cctcgttgac cacccgctcc
    13921 cgcaccgggt gccgggtgtt cgcgagcacc gtcgtgtagc tccagccgcc gtggtcggcc
    13981 accgactccg cggtgggccg gaccgcgccg accggcaccg cggcctcctc ctcggcctcc
    14041 cgggtcgccg cctcgcgcgc cgactcgtgg ccgcgccgcg caccaccggg cagcgcccac
    14101 gtctcgccgt ggtgggtcca ccaggcccgg tgctgcagca acacgccccg ctcgggatcg
    14161 gtcagcagca gcccggcggc gccgaacagg ccccagtgcc gatggccgcg ttcgcagtac
    14221 acgaagccgt cgccgtcatc aggggtcatc gttccagctt gtcacaccgc gcgggcttcg
    14281 agcgcgagcg cggcggcccc caccagggcg gcgtcgtcgc ccagctgcgc ggtgcggatg
    14341 cgcgccagcg gccggtgcct gccgccggtg atcgcggccg cgtagtgctc ccgcgcttcg
    14401 tcgaggaaca gcggcgccga ctccgacacg ccgccggcga tcaccacgat ctccgggtcg
    14461 aacacgtccg cgaccagcgc gagcccctcg ccgagccact tcgccagctc ggccaccgcc
    14521 cgctgcgcga tcgggtcgcc gtcgcgcgcc gcacccgcca cgcggcgccc ggtcagcgcg
    14581 cccgggtcca gcgcgatgtc ccgggacagg atcgtcgagg tgcccgggta ccgggccagc
    14641 agctcgatcg cggtcgcggc cagcgccgtc ccgctgcagt accgctccca gcagccgtac
    14701 ttgccgcacg ggcacgcccg gccgtccggc acgaccgtca agtgccccag ctccggcgcg
    14761 accccgttcg cgccgcggta cagctcgccg tccagcagca gtcccgcgcc gatcccggtg
    14821 ccgatcgcca ccagcgcggc caccttcgcg ccgcgggccg cgccgaagcg gtgctccccg
    14881 accgcggcgg cgttggcgtc gtgctccagc gtcaccggca gcccgacgcg cttggcgatc
    14941 cggtccgcca ccggcgcgtt ccgccacgcc aggtgcggcg cgaacatcac cgtgcggcgg
    15001 tcgttggcga tgaacccggc gaccgcgagc ccgaccccca ccacgtcctc gtgccggttg
    15061 cgcagctcgt cgatcacgcc ggtgatcgcg tcctccagcg cctcctcgcc actcggcgtg
    15121 cccgcccgcg cggtgtccag gatcgagccg tgctcgtcca ccacgccggc gcggacactc
    15181 gtgccgccga cgtccaggcc gatggcgcgc actagccgtc ccgccactgg accgggatgc
    15241 gctgcacccg gccggactcc ggccgcggcc gcggcggcgg ctggaagccg ggcatgtgca
    15301 cgccctcctg cggctcccac cggtcggcca gcaccgcccg cagcaacgcg atgatctgcg
    15361 ccgcctgctc cagcacgcgg gccacgaact cgggcttctc accgcgcacc acggcgacga
    15421 tcgcgcacag cggacaccag ccgcactcgc tgccctgctc cggcgactcg gcggtgccgt
    15481 gcccggccgc gaccaccccg tccagccatg gcgcggcgcg ttcgacgacc atctcgacga
    15541 gcagccggat ctcctcggcc aggctcgccg cggacacccc ttccccggtt gtcacgagtc
    15601 ctcccccggc ttggtcagcg agacgacgag accgtccgca tcggactcgg cgccggtgat
    15661 ccggcacggg cgcaacgctt ccggcagcgc gaccaggcgg cggaacccgt ccacggtgac
    15721 cgccaggtcg tcgtccacac gcgccaggtc cagctcggcg ccgcggtcca gcggcagcgc
    15781 cagccgaagc cggtacccac cgtccacttc ggtcacgtgc agcagcggcg cggactcgga
    15841 gctgttgccg gtcaacggat cccgctcgcc gtacagctcg gtggcgatct cccgcagcgc
    15901 gggcagcccg accggttcgg ccgcccggtg ctcgacccgg gccagctccc acgtcagctc
    15961 ggcgagcacc tcgtcctgct gacgacggcg ggtgcgcatc cagttcgccg ccgcaccccg
    16021 ccacacgccg ggcgcgggca tcagccggtt cacgatcagc ccgtccacgc ggatgccgcg
    16081 cagcgcgagc gagctgagcg tgcggcgtgc ctcggcgacc acgacccgct ccggcgtcag
    16141 caccagccgc accgtggtga ccgccggatc ggtcagcagg tcgcgcagcg acgacacgtg
    16201 cgccgcgagg tcgcggaccg accgggccag accccgggac ccggtgagcg tgagctgcca
    16261 gccgaacacc ttgtccaggt acccggaaac cgcctcggga agcgcgagca gccgcagcgt
    16321 ctccgcggtc ggcccgcagt cgaccaccac gacgtcccag cggcccgcct cggccagccg
    16381 ctgcacctcg gtcagcgcga gcagctcgtc cacgcccggc aggacgctca gctcctcggc
    16441 gtccagggcg tccaccccgg cgccggcgag cgcgcccttg agccggccac gcaggtcctg
    16501 ccagatgtcg tcgaccaggc cgcgcgagtc gacctgcgcg ccgtgcagcc gggtgtccac
    16561 gtcggacggt tcgcggccaa ggcgccggcc gaacgcgtcg cccagcgagt gcgccggatc
    16621 ggtggagacc accagcgtct tgtagccgcg cgcggccagg cccgccgccg tcgccgcggc
    16681 gagcgtcgtc ttgccgacgc ctcccttgcc ggtgaacagc agcagccgcg tcatcgccgc
    16741 cccgccctca ggcgaggtcc tccacgcggc gcttgagttc cttgagcgcg gtgtccatga
    16801 ccatcttctc cgccttgcgg cgcagcagcc cgatcatcgg cagcaccagt tcgaccgaga
    16861 gggtgtaggt gacccgggtg cggccctcgc cgaccgggac cagctcgtag cggccgtcct
    16921 gcgccttctg catctgcccg cgcaccaggt gccagctcac cgagcggccg tcggcggccc
    16981 agtcgtactc cagcgtgtag acgtccttga tcgggccggc gtccagggtc agcttgacct
    17041 gcttggcgcg gccggtgtcg tcggtggcga gcacctcggt ctcccggacc tgcttggccc
    17101 attccgggta cgacgcgaag tccgcgatca ccgccatgac cttctccggc ggcgcgtcga
    17161 cctcgatgga ctgcgtggac tgctcggcca tggcggagag gttaccgcct gacccgcgtc
    17221 accaccgcag gacgtgcggg cgctcggtga ccttgaagtg accgacgtta cggcattcgg
    17281 tgcggccgac ccgcagcctg gccgacagcg gctggtgcac gtggccgaac agcgaccacc
    17341 gcggctgctc cgcgtagacc agcccgcgca gggccgacga gcccagctcg tgccgccgcg
    17401 ccaccacgtc gtaggtcagc tcgggcagcg cgggcggctg gtgggtgcac agcacgtcga
    17461 tgtcggtgag cttgccgacg gcctcgtcga actcctcctg ggtgcgcagg aagggccgcc
    17521 acaccgggtt ggaccggcgg atcgtggccc cggcgggcag caccacgccg ccggcgaagc
    17581 cgaaccgcag gccgccgatc tcgacgacct cgccctcgac cagccgcacg tcgtccccgg
    17641 cgaactccgg ccacaggtcc ggcgagtcga cgttgccggc gatcgcgtag gtcggcgcgg
    17701 tcatcgccgc gaacatcgcg gagtactgct cgcggacggc ctcctcgacg gcggcggccg
    17761 ggtcgtccag gctcgaccac agggacttgg agaacgcgat cgactcctcg cgggtgccct
    17821 cgcggcgcag ccgggcgaac gtggcaacct tttccgggcc gaacagggtg ccgagaatgc
    17881 cctggtcgtg ctggtggtag tcgacgaagt cgagcaggtc gccgaggacg atcagcgcgt
    17941 ccgccccctc accggcccgg gccagtgcct cggtgttgcc gtggacgtcc gagacgacat
    18001 gaacgcgcat gatcccccag tttagcgacg ctaagaagtc cgcgggggaa tcccgggctc
    18061 ccgcccgtcc tccaggatct ccttcaaccc cagcgccacg gccttggccg cccgcgcccg
    18121 gcggtcgaac tcgcggcgca accggcgcgg gctcatctcc gcgaccgggc tcgcgcgcag
    18181 gaagtagtgc agcagcgtgc cgtccagcgt gcgctcgagc cagacctcca tcgtgcccac
    18241 cagcgcgccg cggacggtcc agcgcaggcc ctcgtcgccg cggtcggtgt agacctcgag
    18301 caccaggtcg ggccagtacc gccgccacgc ggccggatcg gcgaacaccg cggccaccgt
    18361 gccgggcggc accgcgagga aggtctcgtc gacgatgtcg atgctgggcg gcgcaccgtt
    18421 gagcgtgctc acgcgagtga gaatgtcatg cccgtgttac cgggggtccg cccccttggt
    18481 cgtaaggtgt caccgacgct aagttgtgac tggcgagtaa cacccctgaa ccacggaggt
    18541 ccaccgtgcg cgagtacagc gccccggcag tgcagccggt tgccgacgac gagaacctcg
    18601 ccgacatcgt gtgggcgaac gccgagcggt tccccgacgc gatcagcttc cggcgccagg
    18661 tggacggctc gtggctggac gtcaccgcgc gcgagttcgc cgcccaggtc atcggggtgg
    18721 cgaagggcct ggtcgaggcc ggtatcgagc ggggtgaccg ggtcgggctg atgtccaaga
    18781 cgcggtacga gtggacgctg atcgacttcg ccatctgggc cgcgggcgcg gtcaccgtcc
    18841 cgatctacga cacctcctcg cccgagcagg tcgcctggat cctgtccgac tccggcgcca
    18901 agggcgtctt cgtggaaacc ggcgagcacc tggccgccgt cgacgaggtc cgcgaccggc
    18961 tgcccgcgct gcggtacacc tggcagatcg aggcggcccg gcccgccgtg gacgagctga
    19021 ccacgctggg cgccgacgtc gccgacgaca ccgtgcacga gcgtcgccgc ctggtgcggg
    19081 ccggcgacct ggccaccatc gtctacacct cgggcaccac cggccgcccc aagggcgtgg
    19141 agctgaccca ccgcaacctg ctcgccgaga tccgggccga catcaacgcc ttcccgcagt
    19201 tgatggagca gggcaactcg ctgctgtgct tcctgccgct ggcgcacgtg ctggcccgcg
    19261 cgatcgccgt caccgccgtc accgcgcgcg tcacgctggg ccacaccccg gacgtcaaga
    19321 acctggtcgc cgacctgggc accttccggc cgaccttcgt ggtggccgtg ccgcgcgtgt
    19381 tcgagaaggt ctacaacggc gcgaagcaga aggcgcactc cgagggcaag ggcaagatct
    19441 tcgacgccgc cgagcaggtg gccgtctcct acagcgaggc gcgggactcc ggcggggcgg
    19501 gcctggcgct gaaggcgcag cacttcctgt tcgacaagct ggtctacggc aagctgcggg
    19561 ccgcgctggg cggccgctgc atcgccgcgg tgtccggcgg cgcgccgctg ggaccccggc
    19621 tggcgcactt cttccgcggc atcggcgtgc cggtgttcga gggctacggc ctgaccgaga
    19681 cctccgccgc ggcgaacgtg aacaccgaac acgccttccg ggtcggcacc gtgggccgcc
    19741 cggtggccgg cacttcggtg cggatcgccg acgacggcga ggtcctgctc aagggcgacg
    19801 tcgtgttcgg cgcctacttc aacaacgaga ccgccacgaa ggaggcgctg gaggacggct
    19861 ggttccacac cggcgacctc ggcgagctcg acgacgacgg cttcctgaag atcaccggcc
    19921 gcaagaagga gatcatcgtg acggccggcg gcaagaacgt cgccccgtcc ggcctcgagg
    19981 acaccatgcg cgcccacccg ctgatcagcc aggccatggt ggtcggcgac cagcgcccgt
    20041 tcatcggcgc gctgatcacg atcgacgagg agttcttccc ggcctggaag tcgcagaacg
    20101 gcaagccggc cgacgccacg gtggccgacc tggcgcagga tccggacctg atcgcggccg
    20161 tgcagggtgc cgtggacgag gcgaacaagc aggtctcgca cgccgaggcg atcaagaagt
    20221 tcgtcatcct gggcaacgac ttcaccgagg ccggcggcga gatcacgccg agcctcaagc
    20281 tcaagcgcaa cgtggtgacc aagaactacg cgacttccat cgaagctctg taccagaagt
    20341 aacgagacta ctacactgtg tcagtgcttc acgatcaccg gtgctgggcc ttcgagtccc
    20401 ccgacgagtt ccaggcccgc gcgcgggagt tcctgcgcga cgggctcgcc cgcggtacgc
    20461 aggtctggtt cgtgaccccg ggcgaacccg tgctcgacgg gctggacgag gcgttggaca
    20521 ccggtgcggc gcgcgtcctc tcgctggacg aaacctactc cgtgggaacc gtggtcgacc
    20581 cggacgccca ggcccgcagc tacgccgagg cgaccgaggc cgcgctggcg gcggggtaca
    20641 ccggcctgcg cgtggcggcg gaccacacgg cgctggtcgc cacgcccgag cagctggacg
    20701 cgttcgcccg ctacgagcac acggtggacc ggtacgcggc cgccgggacg ttctccgcga
    20761 tgtgcggcta cgacctcggc cgggtggacg acgaggtggt ggcgagggtg gcctgcctgc
    20821 acccggtggg caacgccgat ccggggttcc ggctgttcgg gtcggcctcg gcgtccgccg
    20881 cgctcgccgg ggagctggac ctctacaccg aggacctgct gaccgaggcg ctgcggcggg
    20941 cggatccgcg gccggtggac ggacgtctgg tgctcgacgt gagcggcctg cggttcgccg
    21001 accaccggag cctgacccgg ctggccgagc acgccgaggg tgagggtgcc acactggtgt
    21061 tacgcgatcc ctcgccggcc gtcacccgcc tggtcgaagt gctggggctg ccgtcggtgc
    21121 gggtggaggg ggtcgcgtga ccgcgttcgt gcaccccgcg ctgttctact cctccgacga
    21181 ggagtacctg gacgcgctcg tcccgttcgt caccgacggt ctcgcgcggc gggaaccggt
    21241 cgcggtggcc gtgcccggcg accggctgcg ggtgctgcgc ccggcggtgg gcagcgacga
    21301 cgtgctgtgg ctggacatga acgaggcggg ccgcaacccg ggccggatca tcccgacggt
    21361 gctgcgccgg ttcgccgacg cgcacccgga ccggcacgtc cgcatcatcg gcgagccgat
    21421 ctgggccggc cggtcggcca ccgagtaccc ggcgtgcgcg cagcacgagg cgctgatcaa
    21481 ccacgcgttc accgggcggg acgtg
//
