LOCUS       NZ_BNAU01000004        10414 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Amycolatopsis deserti strain CGMCC 4.7677 sequence04, whole genome
            shotgun sequence.
ACCESSION   NZ_BNAU01000004
VERSION     NZ_BNAU01000004.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Amycolatopsis deserti
  ORGANISM  Amycolatopsis deserti
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 638827)
  AUTHORS   Sun,Q. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 82X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014653945.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:43:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,561
            CDSs (total)                      :: 7,498
            Genes (coding)                    :: 7,407
            CDSs (with protein)               :: 7,407
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 32 of 91
            Pseudo Genes (incomplete)         :: 64 of 91
            Pseudo Genes (internal stop)      :: 8 of 91
            Pseudo Genes (multiple problems)  :: 12 of 91
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:49:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 347135
            Orig. end                         :: 357549
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BNAU01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10414
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10414
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10414
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5414](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5414)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    291..306
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.62"
                     /tool="antismash"
     gene            401..553
                     /locus_tag="IE290_RS19630"
                     /old_locus_tag="GCM10017786_39910"
     CDS             401..553
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE290_RS19630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10017786_39910"
                     /product="hypothetical protein"
                     /protein_id="WP_191246071.1"
                     /transl_table=11
                     /translation="MTGTRRGVAVAGTCNWRRWSPPAGGPDGWHPSVVVVLLLAVRPVH
                     LVVHP"
     gene            complement(1086..3794)
                     /locus_tag="IE290_RS19635"
                     /old_locus_tag="GCM10017786_39920"
     CDS             complement(1086..3794)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985330.1"
                     /locus_tag="IE290_RS19635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_39920"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_191246072.1"
                     /transl_table=11
                     /translation="MLKGLRIQLLGPVQLHAGDRPVPIGGPGARGLLAILALNPNRVVA
                     LDDLIDALWHHDPPPTARTIVHGNVSQLRRALRSFETPEARIDTAAPGYRLSIDPMLVD
                     VHRARALFERAEAAPLVERASLLAEAWALWQGPELGGVPVRAPELTHLRIAVHGARVDA
                     DLALGRHEELIAELTTIVREDPRRERTVGQLMRALYYSGRRADALEVYREAARFASAKL
                     GLDPGPDLRALHSQVLNDELAPVASAIDVSRVVPRQLPPAVPLLAGRSADLAWLDGAGA
                     VGVITGPPGIGKSALAVSWAHRAAPRFPDGVLFAELRGFSSPGPVLARFLIGLGVPAAE
                     VPEPEGERLALYRSLTGGRRMLVVLDDARSAEQVRPLLPPGPGSVAVVTSWARLDGLIV
                     SHGARVRSLEPLARADSVRLIADVAGGAFAEFHSQVAGLCGDLPLALRIAGARLAAGPA
                     WTVPEFVAELASERTRLAALDVDDAGVRAALDVSVRGLPAEVAAMFRALGAFPGPTVGP
                     FVAAALCEVPVAEARRRLRVLAAHHLLTESGPEVFTRHDLVRLYAHELAGSSGLPRAVR
                     YYQAAADVARRRLLRIVDPLDFSSVAVPVPSLAGFDDALAWFVAEWPNLLAVLEAAAAA
                     GLDEDVWRLARVAHTYRVVRPLWDEWQRLVALGMAAARACGDVRARFWMLISRCAMSLT
                     FDLGPASLADAAEALSIAADERSHTCAMIHLGCALNSVGRHDEAVECLTKAVAAAGSDE
                     LRGQALANCAEAEKARGRYAEAISYQLSSLEIDRRLGDESYVVVSLNNLAESHLALGEE
                     GVAREYASEAVELASRRGFLLQEAVGRLTLGRVCRRRGDLEEARLQVGLAAELYQRVGP
                     GLAAEVVRELEELGLPVEEPAPSDTRTDAFE"
     misc_feature    3938..3951
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     gene            complement(4041..4934)
                     /gene="thpD"
                     /locus_tag="IE290_RS19640"
                     /old_locus_tag="GCM10017786_39930"
     CDS             complement(4041..4934)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985331.1"
                     /locus_tag="IE290_RS19640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_39930"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_373300479.1"
                     /sec_met_domain="PhyH (E-value: 1.3e-33, bitscore: 112.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METGVEDSYPTRVPVPAEPFDRVDPTVWGSEADGPIDAATLASHD
                     AKGYHIVEGLLSPAEVQGYWQELVRLSSDEQLKADERVITEKKTGSVRSIFEVHKISEL
                     IAELVRDPRVLDRARQILGSEVYIHQSRVNYMPGFKGTGFYWHSDFETWHAEDGMPRPR
                     AVSCSIALTDNYPFNGGLMVMPGSQRTFVPCVGQTPEDYYKSSLKEQEIGVPSENDITK
                     LAAEYGIDQFTGPAGSALWFDSNIMHGSGNNITPYPRSNIFLVFNSVENALVEPYAATK
                     PRPTFIGSRDFTPVTR"
     gene            complement(5001..5414)
                     /locus_tag="IE290_RS19645"
                     /old_locus_tag="GCM10017786_39940"
     CDS             complement(5001..5414)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416992.1"
                     /locus_tag="IE290_RS19645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_39940"
                     /product="ectoine synthase"
                     /protein_id="WP_191246074.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.2e-56, bitscore:
                     184.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLDEITDTDADIKTPNWRSKRIILAKEKVGFSVHETTLYAG
                     TVNDFWYANHIEAVFVYEGEGEITNKATGETFQLKPGSLYLLNDHDKHQVRPKTDMKTV
                     CVFNPPVTGREVHDENGVYPLVVEDDEADEKSA"
     gene            complement(5437..6690)
                     /gene="ectB"
                     /locus_tag="IE290_RS19650"
                     /old_locus_tag="GCM10017786_39950"
     CDS             complement(5437..6690)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 5.9e-73.
                     Score: 237.7. Matches aSDomain:
                     nrpspksdomains_IE290_RS19650_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230319.1"
                     /locus_tag="IE290_RS19650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_39950"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_191246075.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.7e-76, bitscore:
                     250.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFEKLESEVRSYSRGWPVVFDRAQGSRLYGEDGRPYLDFFAGA
                     GALNYGHNNPVLKKALIDYIERDGVTHALDMFTVAKRDLLETLDEKILKPRELDYKVIF
                     PGPGGANAVEAALKLARKVTGRESVINFTNAFHGMTIGALSVTGNSMKRHGAGIPLVHA
                     TPMPYDQYFDGVYPDFLYFERLLQDSGSGLNEPAAVIVETVQGEGGINAAGLEWLRGLS
                     DLCKRHDILLIVDDVQMGCGRTGPFFSFEDAGIKPDIVCLSKSISGYGLPMALTLIRPD
                     LDVWEPGEHNGTFRGINPAFVTAAEAMRTYWSDDELEKSVRAKGERIGAALQGLVDAYP
                     EAQLTAKGRGLARGLEFQNGELAGKVCAAAFERGLLMETSGPDSEVMKLLPPLTLTDAE
                     VGEGLEIIDASVKAVLGK"
     aSDomain        complement(5617..6627)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE290_RS19650_Aminotran_3.1"
                     /evalue="5.90E-73"
                     /label="IE290_RS19650_Aminotran_3.1"
                     /locus_tag="IE290_RS19650"
                     /protein_end="358"
                     /protein_start="21"
                     /score="237.7"
                     /tool="antismash"
                     /translation="FDRAQGSRLYGEDGRPYLDFFAGAGALNYGHNNPVLKKALIDYIE
                     RDGVTHALDMFTVAKRDLLETLDEKILKPRELDYKVIFPGPGGANAVEAALKLARKVTG
                     RESVINFTNAFHGMTIGALSVTGNSMKRHGAGIPLVHATPMPYDQYFDGVYPDFLYFER
                     LLQDSGSGLNEPAAVIVETVQGEGGINAAGLEWLRGLSDLCKRHDILLIVDDVQMGCGR
                     TGPFFSFEDAGIKPDIVCLSKSISGYGLPMALTLIRPDLDVWEPGEHNGTFRGINPAFV
                     TAAEAMRTYWSDDELEKSVRAKGERIGAALQGLVDAYPEAQLTAKGRGLARGLEFQ"
     gene            complement(6759..7259)
                     /gene="ectA"
                     /locus_tag="IE290_RS19655"
                     /old_locus_tag="GCM10017786_39960"
     CDS             complement(6759..7259)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033456.1"
                     /locus_tag="IE290_RS19655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_39960"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_191246076.1"
                     /transl_table=11
                     /translation="MSGKHLIENPTKADGAALWRIARDSQKLDLNSPYAYLLWCRDFAD
                     TSVVARVDGDAVGFVIAYRRPTAPDTALVWQVAVDASQRGQGLAGALLDNLFTRLVADG
                     VRHLETTITPDNKASIRLFTSFAQRWGAQLECTELFGSDDFPDDGTRHEREDLYRIGPL
                     SPR"
     misc_feature    complement(7260..7275)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.96"
                     /tool="antismash"
     misc_feature    complement(7336..7351)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.99"
                     /tool="antismash"
     misc_feature    complement(7459..7473)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.35"
                     /tool="antismash"
     misc_feature    7460..7474
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.41"
                     /tool="antismash"
     gene            complement(7469..8245)
                     /locus_tag="IE290_RS19660"
                     /old_locus_tag="GCM10017786_39970"
     CDS             complement(7469..8245)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985335.1"
                     /locus_tag="IE290_RS19660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_39970"
                     /product="Abi-alpha family protein"
                     /protein_id="WP_191246077.1"
                     /transl_table=11
                     /translation="MWLTSENLPSSPEGGDENLLRRAGRLAGWAARTGFTIGRRLPLPG
                     LELAGEGLRQVERQVLTGLRRRLDEVDDPYVVALSAAGSEHTPNGAGELVPAREPLRAA
                     MKELLDRSVGFGREQAREYLYATVLRQLTPDEARIIATLATGAAFPVIDVTERGFGGGR
                     TILRNASTVGKAAGVTLPDEVPGYLTRLAGFGLVELDAESAELESQYEILATEDLVRTA
                     VKSARRARLVRRTVRLSRFGVRFWAACDPGEWARIL"
     gene            complement(8279..8623)
                     /locus_tag="IE290_RS19665"
                     /old_locus_tag="GCM10017786_39980"
     CDS             complement(8279..8623)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985336.1"
                     /locus_tag="IE290_RS19665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_39980"
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="WP_191246078.1"
                     /transl_table=11
                     /translation="MTHAQGDEPATGTPPQPPVTEPAPAKPVKIKRTRVSGTWVAIVVA
                     LIILVFLLIFILQNLDSTTVAFLGMEGSLPLAVAMLFSAIAGAVLVALVGGARIFQLRK
                     ATRKANKALR"
     gene            complement(8666..9460)
                     /locus_tag="IE290_RS19670"
                     /old_locus_tag="GCM10017786_39990"
     CDS             complement(8666..9460)
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416997.1"
                     /locus_tag="IE290_RS19670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_39990"
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="WP_191246079.1"
                     /transl_table=11
                     /translation="MADAVRRWSEQLAGWAIPAHILVAAPESPWFVPRRAFARRADRLI
                     AEPATPTHDAVLAGLPGSVLDVGAGAGAASLPCARAITRVTAVDTNAGLLAEFTARAEA
                     LSVPCTTIEGRWPDVEAAPADVVVCAHVLYNAPDLAPFTEALTAHARRRVVVELAAVHP
                     MTSLNALWRRFHGIDRPDGPTADDAVAALRELGVEPEIVRWHQPAKREYQRFDELVDVT
                     RRRLCLPPDRADEVAEALRGAPDFGSAGRDVVTLSWRVSRSV"
     gene            9524..10090
                     /locus_tag="IE290_RS19675"
                     /old_locus_tag="GCM10017786_40000"
     CDS             9524..10090
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416998.1"
                     /locus_tag="IE290_RS19675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10017786_40000"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_191246080.1"
                     /transl_table=11
                     /translation="MPTTDARERLLTVAERLLLESGYDAVSVRAINSAAGMNPAAVHYH
                     FGSKDALIAALLEARLAPVWQQRLDEVTSQRRDGWAPTVPELVDLVVTPLAELAADPVG
                     QLRLKLLARFVLGRREPAWTSRWFGLGPWIELLRDARPELSKAEAAQRWLLAFGLVLQF
                     FAEGMPGEVPVETLRAFVAAGLAGS"
     misc_feature    complement(10190..10205)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.43"
                     /tool="antismash"
ORIGIN
        1 gcagcatgac aaccgagcag gcgaaggtgc tgcacaacag cctgcgggag tcgcgcgaag
       61 aggtcggcta cgagacggtg tccggccgcc tggacggggt ccgcacgcgc cgccggatct
      121 tctacctgga gcgcgagtcc ggggggacga tccagggggc ggtggcgccg gacctgctgg
      181 accagatcaa ggaaaacctg gaccgcccgg tgacggcccg cctacgggta gtccgcacca
      241 cgacgatcga cggccgccgc ggccgcccgg tgcacgagct gctggagatc acgccggaag
      301 tgaacctgtt cgaccagtga cgcgtggctg gtggcggctg gcctaggggc tggggtcgac
      361 gggcggtggc ggcggtgatc gccgcccgcc ggtggtcgag gtgaccggca cccgccgggg
      421 cgtggcggtg gccggcacct gcaactggcg gcggtggtcg ccgcccgccg gtggtccgga
      481 tggctggcac ccgtcggtgg tggtggtgct gctgcttgcc gtccgcccgg tgcacctggt
      541 ggtccacccg taaatggtgc cggtgatcgt ctgcccgatc ggtcctggtg gctggtgccg
      601 aggggtggtg gtcctggtcg cctggctggc ggagggcccg gtgctggcac cctcgtgaat
      661 ggaccgttca tcggcgcgcg aaatgaccac tcgcgccagc gcggcgacac gagaggcacc
      721 ctcgccccat cgcgagaggt ccactcacgg ccgcatgagc ggtccacaca ccaccgcccg
      781 aaaggtccac tcgcggccac atgagcggtc cactcaccac cgcgcgagag gtccgctcgc
      841 ggcgacaacc ggggggccgc tcaaggcgct taccgctggt catgtcgtgc ggcgcgaatc
      901 caccagccgg tgcagcgatg aacggtccat tcatcgcttc ccgtgggcgc ccgggtcggc
      961 gacagggcca accagcggaa tcgagacggc cgtctgacgc tcccccactg cgccagacgg
     1021 ccaagcggca ccatacgccc tcggcaagat ccatcgctaa gaattcgcta agtgcgggga
     1081 ttcttttact cgaaagcgtc agttcgagtg tccgacgggg ccggctcctc gaccggcagg
     1141 cccagctcct ccagctcccg caccacctcc gcggccagcc cgggccccac ccgctggtac
     1201 agctccgccg ccagcccgac ctgcaaccgc gcctcctcga ggtcgcctcg gcggcggcac
     1261 acccggccga gcgtcaggcg cccgaccgcc tcctgcagca ggaaaccccg ccgggacgcc
     1321 agctccaccg cctcggacgc gtactcccgc gccacacctt cctcacccag ggcgaggtgc
     1381 gactccgcca ggttgttcag tgacaccacc acataacttt cgtcccccag tcgccggtcg
     1441 atctccagcg acgacagctg atacgaaatc gcctcggcgt accggcccct cgccttctcc
     1501 gcctccgcgc agttcgccag cgcctgcccc cgcagctcgt ccgaacccgc ggccgccacc
     1561 gccttcgtca ggcactccac cgcctcgtcg tgacggccca cgctgttcaa cgcgcacccc
     1621 aggtggatca tcgcgcaggt gtggctccgc tcgtccgccg cgatcgacag cgcctcggcc
     1681 gcatccgcca gcgacgccgg ccccagatcg aacgtcaggg acatcgcaca ccgcgagatc
     1741 agcatccaga accgcgcccg cacatccccg cacgcccggg ccgccgccat ccccagcgcc
     1801 accagccgct gccactcgtc ccacagcggg cgcaccaccc ggtacgtgtg cgccacccgc
     1861 gccagccgcc agacgtcctc gtccaacccg gccgccgccg cggcctccag caccgccaag
     1921 aggttcggcc actcagccac gaaccacgcc agcgcgtcgt cgaaaccggc caacgaaggc
     1981 accggcaccg caacagaaga aaaatccagc gggtccacga tccgcaacaa ccgccgccgc
     2041 gcgacgtcgg ccgccgcctg gtagtaccgc accgcccgcg gcaaccccga cgaaccagcc
     2101 agttcgtgcg cgtacaaccg caccaggtcg tgccgcgtga acacctccgg cccgctctcg
     2161 gtcagcaggt ggtgcgccgc cagcacccgc agccgccgcc gcgcctccgc gaccggcacc
     2221 tcgcacaacg ccgccgccac gaacggcccg accgtcggcc cgggaaacgc ccccagcgcc
     2281 cggaacatcg ccgccacctc ggccggcaac ccccgcaccg acacgtccag cgccgcccgc
     2341 accccggcgt cgtcgacgtc cagcgccgcg agccgcgtcc gctcgctcgc caactcggcc
     2401 acgaactccg gcaccgtcca cgccggaccc gccgccagcc gcgcccccgc gatccgcaac
     2461 gcgagcggca ggtccccgca caacccagcc acctgcgagt ggaactccgc gaacgcccca
     2521 ccggcgacat ccgcgatcaa ccgcaccgag tccgcccgcg ccaacggctc cagcgaccgc
     2581 acccgcgccc cgtgcgaaac gatcaggccg tccagccgcg cccacgaggt caccaccgcg
     2641 accgaccccg gccccggcgg caacaacggc cgcacctgct ccgccgaccg ggcgtcgtcg
     2701 agtaccacga gcatccgccg tccgccggtc agcgaccggt acagcgcgag ccgctccccc
     2761 tccggctccg gcacctcggc cgcgggcacc cccagcccga tcaagaaccg cgccagcacc
     2821 ggccccggtg aagagaaccc gcgcaactcg gcgaacagca ccccgtccgg aaaccgcgga
     2881 gcggcccggt gcgcccacga caccgcgagc gcgctcttgc cgatcccggg cggcccggtg
     2941 atcaccccga ccgcgccggc accgtccagc caggccaggt ccgccgaccg cccggccagc
     3001 aacggcaccg cgggcggcaa ctggcgcggc accacccggc tcacgtcgat cgcggaggcg
     3061 accggcgcca gttcgtcgtt caacacctgc gaatgcagcg ctcgcaggtc cggcccggga
     3121 tcgaggccga gcttcgccga cgcgaaccgc gccgcctccc ggtagacctc gagcgcgtcg
     3181 gcccggcgcc ccgagtagta cagcgcgcgc atcagctggc ccacggtgcg ctcgcgccgc
     3241 ggatcctccc ggacgatcgt ggtcagctcg gcgatcagct cctcgtgccg cccgagcgcg
     3301 agatcggcgt ccacccgggc gccgtgcacc gcgatccgca aatgcgtcaa ttcgggcgcg
     3361 cgcaccggca ccccgcccag ctccggcccc tgccacagcg cccacgcctc ggccagcagc
     3421 gaggcgcgtt cgacgagcgg cgccgcctcg gcgcgctcga acagcgcccg cgctcggtgc
     3481 acgtcgacca gcatcgggtc gatgctcagc cggtaaccgg gcgcggcggt gtcgatccgc
     3541 gcctccgggg tctcgaacga ccgcaacgcc cgccgcagct gggagacgtt gccgtgcacg
     3601 atcgtccgcg ccgtcggcgg cgggtcgtgg tgccacagcg cgtcgatcag gtcgtcgagc
     3661 gcgacgacgc ggttcgggtt cagcgccagg atcgcgagca gcccgcgcgc gcccggtccg
     3721 ccgatcggca ccggccggtc gccggcgtgg agctggacag gtccgagcag ctggatgcgc
     3781 aaacccttga gcacaggccc ctccccgagc accgcgccgg aggaaacgag tacggccccg
     3841 caccatcagt cccgatctga tggtgcgggg ccgcactcgg tgaaacaagt gacggggccg
     3901 ggttcgcctc gcccatcgct accggcgcca cacgggattc accccgagca aggaagaccc
     3961 atgctgccgc cgttcgcagt ggcggcactg taacagggcg cgatgaaccg gctacacccg
     4021 acccgcactt cttccggtgt ctaccgcgtc acaggcgtga agtcccggct gccgatgaag
     4081 gtcggccggg gcttcgtcgc cgcgtacggc tcgacgagcg cgttctcgac gctgttgaac
     4141 accaggaaga tgttggaccg cgggtacggc gtgatgttgt tgccggaccc gtgcatgatg
     4201 ttggagtcga accacagcgc cgacccggcc ggcccggtga actggtcgat cccgtactcc
     4261 gcggcgagct tggtgatgtc gttctcgctg ggaacgccga tctcctgctc cttcagcgac
     4321 gacttgtagt agtcctccgg ggtctggccg acgcacggga cgaacgtccg ctgcgaaccc
     4381 ggcatgacca tcaggccgcc gttgaagggg tagttgtcgg tcagcgcgat cgagcagctc
     4441 accgcccgag gccgcggcat gccgtcctcg gcgtgccagg tctcgaagtc ggagtgccag
     4501 tagaacccgg tgcccttgaa gcccggcatg tagttcacgc ggctctggtg gatgtacacc
     4561 tccgagccga ggatctgccg ggcgcggtcg aggacgcggg ggtcgcgcac gagttcggcg
     4621 atcagctcgc tgatcttgtg cacctcgaag atggaccgga cgctgccggt cttcttctcg
     4681 gtgatgaccc gctcgtcggc cttcagctgc tcgtcgctgg agagccggac gagctcctgc
     4741 cagtagccct gcacttcggc gggcgacagc aggccctcga cgatgtggta ccccttcgcg
     4801 tcgtgcgacg cgagagtcgc cgcatcgatc ggaccgtcgg cctcactgcc ccagacagtc
     4861 gggtcgacac gatcgaaggg ctcggccggg acgggaaccc tggtcgggta actgtcctcg
     4921 acaccggttt ccatgatcgt caaatcggtt cgcctccttt tatgcgttgc cttccggtag
     4981 gggcctcgct ccgtggtggc tcaggcggac ttctcgtccg cctcgtcgtc ctcgacgacc
     5041 agcgggtaca cgccgttctc gtcgtgcacc tcccggccgg tgaccggcgg gttgaagacg
     5101 cacacggtct tcatgtcggt cttcgggcgg acctggtgct tgtcgtggtc gttgaggagg
     5161 tacagggaac ccggcttgag ctggaacgtc tcgccggtcg ccttgttggt gatctcgcct
     5221 tcgccctcgt agacgaagac cgcctcgatg tggttggcgt accagaagtc gttgacggtc
     5281 cccgcgtaca gcgtggtctc gtgcaccgag aagccgacct tctccttggc caggatgatc
     5341 cgcttcgagc gccagttcgg cgtcttgatg tcggcgtcgg tgtcggtgat ctcgtcgagg
     5401 gtccggacga tcaaaactct tccccttctc gttcttctac ttaccgagca cggccttgac
     5461 ggaggcgtcg atgatctcca gcccctcgcc gacctcggca tccgtgagcg tcagcggcgg
     5521 cagcagcttc atcacctcgc tgtccggccc ggaggtctcc atgagcaggc cgcgttcgaa
     5581 cgcggcggcg cagaccttcc cggcgagttc cccgttctgg aactccaaac cgcgggccag
     5641 gccacggccc ttggcggtca gctgcgcctc cgggtaggcg tcgaccaggc cctgcagcgc
     5701 cgcgccgatg cgctcgccct tggcccggac cgacttctcc agctcgtcgt cgctccagta
     5761 ggtgcgcatg gcctcggcgg cggtgacgaa cgccgggttg atgccgcgga acgtgccgtt
     5821 gtgctcgccc ggctcccaga cgtccaggtc ggggcggatc agggtgagcg ccatcggcag
     5881 gccgtagccg ctgatcgact tcgacaggca gacgatgtcc ggcttgatgc cggcgtcctc
     5941 gaagctgaag aacgggccgg tgcggccgca gcccatctgc acgtcgtcga cgatcagcag
     6001 gatgtcgtgc cgcttgcaca ggtcggacag gccgcgcagc cactccaggc cggcggcgtt
     6061 gatgccgccc tcgccctgca cggtctccac gatcaccgcg gccggctcgt tcagcccgct
     6121 gccggagtcc tggagcaggc gctcgaagta caggaagtcc gggtagacgc cgtcgaagta
     6181 ctggtcgtag ggcatcggcg tggcgtggac cagcgggatg ccggcgccgt ggcgcttcat
     6241 cgagttgccg gtgaccgaca gcgcgccgat cgtcatgccg tggaaggcgt tggtgaagtt
     6301 gatgaccgac tcgcggccgg tgaccttgcg ggccagcttc agcgcggctt cgacggcgtt
     6361 ggcgccgccg gggccgggga agatgacctt gtagtccagc tcgcgcggct tgaggatctt
     6421 ctcgtcgagc gtctcgagca ggtcgcgctt ggcgacggtg aacatgtcga gcgcgtgcgt
     6481 gacgccgtcg cgctcgatgt agtcgatcag cgccttcttg agcacggggt tgttgtggcc
     6541 gtagttcagc gccccggcgc cggcgaagaa gtcgaggtac gggcgcccgt cctcgccgta
     6601 gaggcggctg ccctgcgcgc ggtcgaacac gaccggccac ccccggctgt agctgcgcac
     6661 ttcggattcg agcttttcga agatgctcac tttgggtttt cccctcactg gtgcttgtcg
     6721 acagctaatc gcacgggcgt cctcgcccaa ttccggcgtt atctcgggct cagcgggccg
     6781 attcggtaaa ggtcttcccg ctcgtgccgc gtgccgtcgt ccggaaagtc gtccgagccg
     6841 aacagctcgg tgcactccag ctgtgcgccc caccgctgcg cgaatgaggt gaacagccgg
     6901 atggacgctt tgttgtcggg cgtgatggtg gtctcgaggt gacgcacccc gtcggcgacc
     6961 agccgcgtga acaggttgtc gagcagggct ccggccagcc cctgcccacg ttgcgacgca
     7021 tcgacggcga cctgccagac gagcgcggtg tccggcgccg tgggacggcg ataagcgatc
     7081 acgaatccga ccgcgtcacc gtccacccgc gccaccaccg aggtgtcggc gaaatcgcgg
     7141 caccacaaca gatatgcata aggcgagttg agatcgagct tctgcgaatc gcgtgcgatt
     7201 cgccaaagcg cagccccgtc ggccttggtc ggattttcga tcaagtgctt tccggacatg
     7261 tcaaaacaac ctaacagagt tgtttgggtc gttcagcgca tcgcggaccg ggcaggcgag
     7321 ctacctgcaa caacggcgaa acacacgtga gaatgacaac aggaagctgg tgttccgaag
     7381 ccgagatgtg gtatggcccc agcctggacg gggccttatc acgtagagcg ctcgacgtgc
     7441 gccgcgcgtt acacgctagt ggcagcgctc acaggatgcg agcccactca ccgggatcgc
     7501 acgccgccca gaagcggacg ccgaaccgcg acaggcggac cgtgcgccgc accaggcgtg
     7561 cccggcgggc ggacttcacg gccgtgcgga cgaggtcctc ggtggccagg atctcgtact
     7621 gcgactccag ctcggcggac tcggcgtcca gctcgaccag gccgaatccg gccagccggg
     7681 tcaggtagcc gggcacctcg tcgggcagcg tcacaccggc cgccttgccc accgtcgagg
     7741 cgttgcgcag gatcgtccgg ccgccgccga acccgcgttc ggtgacgtcg atcacaggga
     7801 aggcggcgcc ggtggcgagc gtggcgatga tgcgcgcctc gtccggggtg agctggcgca
     7861 ggacggtcgc gtagaggtac tcgcgggcct gctcccggcc gaagcccacg gaccggtcga
     7921 gcagctcctt catcgcggcg cgcagcggtt cccgcgccgg gaccagctcg ccggcgccgt
     7981 tgggggtgtg ctcggagccg gccgcgctga gcgcgaccac gtacgggtcg tcgacctcgt
     8041 cgagacggcg gcgcaggccg gtcaggacct gccgctcgac ctgtcgcagc ccctcaccgg
     8101 ccagttcgag accgggcagc ggcagacgac ggccgatggt gaacccggtg cgtgcggccc
     8161 agccggcgag ccgtcccgcg cggcgcagca ggttctcgtc gccaccctcc ggcgaactcg
     8221 gcaggttctc gctggtcaac cacatcaccc cgtttcccgg gatgatggta cggccggatc
     8281 agcgcagagc cttgttcgcc ttccgcgtcg ccttgcggag ctggaagatg cgcgcgccgc
     8341 cgaccagggc gaccaggacg gctccggcga tcgcggagaa cagcatcgcc acggccagcg
     8401 gcaggctgcc ctccatcccg aggaaggcca ccgtcgtcga gtccaggttc tgcaggatga
     8461 agatcagcag gaacaccagg atgatcaacg cgacgacgat cgccacccag gtgccgctga
     8521 cccgggtccg tttgatcttg accggcttcg cgggcgcggg ttcggtgacg ggcggctggg
     8581 gtggggtgcc ggtggccggc tcgtccccct gcgcgtgagt catacaccga ttgtgctcgg
     8641 gtgggcggat tcccgcaggt cgaaatcaca cggagcggct aactcgccag ctgagcgtga
     8701 ccacatcgcg acccgcggag ccgaagtcgg gtgcgccgcg cagcgcttcg gccacctcat
     8761 cggcccggtc cgggggcagg cacagccggc ggcgggtgac gtcgaccagc tcgtcgaagc
     8821 gctggtattc ccgcttcgcg ggctggtgcc accggacgat ctccggttcg acgccgagtt
     8881 cccgcagcgc ggcgacggcg tcgtcggccg tcgggccgtc cgggcggtcg atgccgtgga
     8941 accgccgcca caacgcgttc aggctggtca tcgggtggac ggcggcgagt tctacgacga
     9001 cccggcggcg ggcgtgggcg gtcagcgcct cggtgaacgg cgccaggtcg ggggcgttgt
     9061 agaggacgtg ggcgcagacc acgacgtcgg cgggtgcggc ctcgacgtcc ggccaccgcc
     9121 cctcgatcgt cgtgcagggc acggagagcg cttccgcgcg ggcggtgaac tcggcgagca
     9181 ggccggcgtt ggtgtccacc gccgtgaccc gcgtgatggc ccgggcgcac ggcaggctcg
     9241 ccgcgcccgc cccggcaccc acgtcgagca cggaaccggg aagaccggcc aggacggcgt
     9301 cgtgggtcgg ggtggcgggt tcggcgatca ggcggtcggc gcggcgcgcg aacgcccggc
     9361 ggggcacgaa ccacggcgat tcgggcgccg cgaccaggat gtgcgccggg atcgcccagc
     9421 ccgccagctg ctccgaccac ctgcgcaccg cgtccgccat gggttcgatt gtgccggtca
     9481 atcagtgatt gattcagtga tggaagtgtg gtctgctgga cgggtgccga ccaccgacgc
     9541 ccgcgagcga ctgctgaccg tcgccgagcg gctgctgctg gaatccggct acgacgcggt
     9601 gtcggtgcgg gcgatcaaca gcgcggccgg catgaacccg gcggcggtgc actaccactt
     9661 cgggtcgaag gacgcgctga tcgcggcgct gctggaggcc cggctcgcac cggtgtggca
     9721 gcagcggctg gacgaggtca cctcgcagcg gcgcgacggg tgggcgccga ccgtgcccga
     9781 actggtggat ctggtcgtga ccccgctggc cgagctggcc gcggatccgg tcgggcagtt
     9841 gcggttgaag ctgctggcgc ggttcgtgct ggggcggcgg gaaccggcgt ggacgtcgcg
     9901 gtggttcggg ctggggccgt ggatcgagct gctgcgggac gcgcggccgg agctgtccaa
     9961 ggcggaggcg gcgcagcgct ggctgctcgc gttcgggctg gtgctgcagt tcttcgccga
    10021 ggggatgccc ggcgaggtgc cggtggagac gctgcgggcg ttcgtcgcgg cgggattggc
    10081 ggggtcatga ccgacgtctt cgcacccgcg cgactggggc cggtcgagct gcggaaccgg
    10141 atcatcaagg cggccacgtt cgagggcgcg accccggacg cgctggtcac tgaccagttg
    10201 atcgagttcc accggcggcc ggcgcgcggc ggggtcggga tgaccacggt ggcgtactgc
    10261 gcggtgtcgc cggaggggcg caccgaccgg caccagatct ggatgcgcga ggaggcgctg
    10321 ccggggctgc ggcggctcac cgacgcggtg cacgccgagg gtgcggcggt gagcgcgcag
    10381 atcgggcacg cggggccggt cgcgaacgcg gcgt
//
