LOCUS       NZ_BMWB01000023        10399 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces xantholiticus strain JCM 4863 sequence23, whole genome
            shotgun sequence.
ACCESSION   NZ_BMWB01000023
VERSION     NZ_BMWB01000023.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces xantholiticus
  ORGANISM  Streptomyces xantholiticus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 127781)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 114X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014651015.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,091
            CDSs (total)                      :: 7,016
            Genes (coding)                    :: 6,693
            CDSs (with protein)               :: 6,693
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 323
            CDSs (without protein)            :: 323
            Pseudo Genes (ambiguous residues) :: 0 of 323
            Pseudo Genes (frameshifted)       :: 187 of 323
            Pseudo Genes (incomplete)         :: 270 of 323
            Pseudo Genes (internal stop)      :: 13 of 323
            Pseudo Genes (multiple problems)  :: 140 of 323
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:39:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 52754
            Orig. end                         :: 63153
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMWB01000023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            83..691
                     /locus_tag="IE198_RS27330"
                     /old_locus_tag="GCM10010381_55500"
     CDS             83..691
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="IE198_RS27330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55500"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_189891230.1"
                     /transl_table=11
                     /translation="MELKLPDLPFPLRAYGPEADWSYEDGVLTAWAGPRQDRFVPPTGH
                     SLEPASDAPRLLGAPEGDFQLIARVKVGFAAAFDAGVLYLHVTEQEWAKLCLELSPDRP
                     TVCTVVTRGHSDDANSFVVDGDSVWLRISRTGKAFAFHASTDGERWTFVRIFTLGDEDR
                     AGAALVGFMAQSPVGEGCVVSFDRIEYRDSWPEGLRDGS"
     gene            complement(744..1178)
                     /locus_tag="IE198_RS27335"
                     /old_locus_tag="GCM10010381_55510"
     CDS             complement(744..1178)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319050.1"
                     /locus_tag="IE198_RS27335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55510"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_189891232.1"
                     /transl_table=11
                     /translation="MTPQTDHIEITTLVSRLFRALDARKFADGWADGYFTGDIRMIAPV
                     GVYDGPAAVQGTEEAVGRFARTQHMASDVLTDVDSASGTARASWNALMVHLHHEGPDPL
                     FTVGGRYEADLRRTDDGWRFRRMSVEAVWTTGRPPVLPSA"
     gene            1284..1757
                     /locus_tag="IE198_RS27340"
                     /old_locus_tag="GCM10010381_55520"
     CDS             1284..1757
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885292.1"
                     /locus_tag="IE198_RS27340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55520"
                     /product="N-acetyltransferase"
                     /protein_id="WP_189891234.1"
                     /transl_table=11
                     /translation="MIQIAAAADLDAIAALHAEARATYYWGHIPDEAFDSPAEHARTRT
                     GWENAIGRGAVLCAIRDGAVCGVAAFGDRKGVMTLSQLHVAPGHWRSGVGSELHAAVVA
                     AWQRDQVTTARLEVFEKNERAQAFYTHHGWYPDPQDPHAGNHLILRFSVPSAA"
     gene            1993..2718
                     /locus_tag="IE198_RS27345"
                     /old_locus_tag="GCM10010381_55530"
     CDS             1993..2718
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518879.1"
                     /locus_tag="IE198_RS27345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55530"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_189891236.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEKGLEAFAHRDEIEVVHRSFELDP
                     NRAGHDTTPVVDLLAKKYGRTREEARAMEEHVASTARSEGLEYRTEGRDHGNTFDIHRL
                     LHLAKARGLQDELLTLVYRANFAEERSVFDPEVLVALAAEAGLDEQEARRALADEAAYA
                     DDVRADEREAAELGATGVPFFVFDRRYGVSGGQPAELFTQALEQAWQGRSVTPPGGAAA
                     CDADGACEVPAAATEATVH"
     gene            2791..3852
                     /locus_tag="IE198_RS27350"
                     /old_locus_tag="GCM10010381_55540"
     CDS             2791..3852
                     /NRPS_PKS="Domain: Aminotran_5 (117-292). E-value: 1.6e-23.
                     Score: 75.1. Matches aSDomain:
                     nrpspksdomains_IE198_RS27350_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567664.1"
                     /locus_tag="IE198_RS27350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55540"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_189891238.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.8e-21, bitscore:
                     69.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEISKPLIDAEFAPRTTYLNTAVNGLLPRRSVHAVKALAQELGEG
                     RPGGSGDYSAVTAARRSFARLTGVDEGRVAIGGSVSVHAGLIAASLRSGAEVLLPEGEF
                     TSLVTPFAVRGDLKLRQVPLDELAEAVRPGTDLVALSSVQSADGRVADLVAVRAAAAAH
                     GARTLLDASQSAGWLPLDAAAWDFTVTAGYKFLLCPRGVSFLTVTEEAQESLPPAHAGT
                     FAAERPGQADSMYGPITELARSARRYDETPAYLPYHAAEQSLALLEEIGIQTVHAHDTA
                     LAGRFSAGLRRHGLEPVAGESAIVAVPGLGDRAEELCRAGILVSGRAGNLRVTFHLYNS
                     PADVERALDFLVG"
     aSDomain        3142..3666
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE198_RS27350_Aminotran_5.1"
                     /evalue="1.60E-23"
                     /label="IE198_RS27350_Aminotran_5.1"
                     /locus_tag="IE198_RS27350"
                     /protein_end="292"
                     /protein_start="117"
                     /score="75.1"
                     /tool="antismash"
                     /translation="KLRQVPLDELAEAVRPGTDLVALSSVQSADGRVADLVAVRAAAAA
                     HGARTLLDASQSAGWLPLDAAAWDFTVTAGYKFLLCPRGVSFLTVTEEAQESLPPAHAG
                     TFAAERPGQADSMYGPITELARSARRYDETPAYLPYHAAEQSLALLEEIGIQTVHAHDT
                     ALAGRFSAGLRR"
     gene            complement(4100..4996)
                     /gene="thpD"
                     /locus_tag="IE198_RS27355"
                     /old_locus_tag="GCM10010381_55550"
     CDS             complement(4100..4996)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="IE198_RS27355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55550"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_189891240.1"
                     /sec_met_domain="PhyH (E-value: 5.9e-38, bitscore: 124.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPARTDLYPTRGASEVLTPRKDPVVWSAPGSLGPIDTADLMG
                     FERDGFLTVDQLIGDDEVARYHSELERLISDPMVRADERSIIEPKSQDVRSVFEVHKIS
                     EVFASLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPN
                     MRTVSVSIALTQNFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEAL
                     TRFADDHGIRLFTGRAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENAAVEPFAA
                     PVPRPEFIGARDFTPVR"
     gene            complement(5001..5399)
                     /locus_tag="IE198_RS27360"
                     /old_locus_tag="GCM10010381_55560"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="IE198_RS27360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55560"
                     /product="ectoine synthase"
                     /protein_id="WP_100106535.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.4e-56, bitscore:
                     180.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTERHVKSASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGETHWIEPGTMYLLDGHEKHTLRPKTDFR
                     VVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            complement(5430..6698)
                     /gene="ectB"
                     /locus_tag="IE198_RS27365"
                     /old_locus_tag="GCM10010381_55570"
     CDS             complement(5430..6698)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 1.2e-70.
                     Score: 230.1. Matches aSDomain:
                     nrpspksdomains_IE198_RS27365_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="IE198_RS27365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55570"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_189891243.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-76, bitscore:
                     251.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGSHLHDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLESFQNVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALNTYWADGQMEKQTIARGEQVERALL
                     AIVDEMGGEGVSFRGRGLVWGMEFDDKPRASAVCARAFELGLLLETSGPESEVVKLLPP
                     LTISSDELDQGLRIVARAVRETA"
     aSDomain        complement(5604..6614)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE198_RS27365_Aminotran_3.1"
                     /evalue="1.20E-70"
                     /label="IE198_RS27365_Aminotran_3.1"
                     /locus_tag="IE198_RS27365"
                     /protein_end="365"
                     /protein_start="28"
                     /score="230.1"
                     /tool="antismash"
                     /translation="FDRAQGSHLHDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLESFQNVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALNTYWADGQMEKQTIARGEQVERALLAIVDEMGGEGVSFRGRGLVWGMEFD"
     gene            complement(6792..7322)
                     /gene="ectA"
                     /locus_tag="IE198_RS27370"
                     /old_locus_tag="GCM10010381_55580"
     CDS             complement(6792..7322)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319066.1"
                     /locus_tag="IE198_RS27370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55580"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_189891245.1"
                     /transl_table=11
                     /translation="MTAAQEDLVRARDEFLEIGTPRVEDGAAIWRIARDSEVLDLNSSY
                     SYLLWCRDFAATSVVARGRDGEPIAFVTGYVRPDRPEALVVWQVAVDRAHRGRGLAGVL
                     LDALTAKVTGGHGVREIETTITPDNTASDALFTSYAKRHGASLSREVLFDGGLFPEGTH
                     EPEVLYRIGPVAA"
     misc_feature    complement(7331..7345)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.34"
                     /tool="antismash"
     misc_feature    7413..7426
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
     misc_feature    complement(7477..7492)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7487..7502
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7592..7606)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.85"
                     /tool="antismash"
     misc_feature    7664..7679
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            complement(7679..>7960)
                     /locus_tag="IE198_RS35610"
                     /pseudo=""
     CDS             complement(7679..>7960)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018533391.1"
                     /locus_tag="IE198_RS35610"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="HAARDAAPVATTPRAPAGVDARDLVAKAWPPTRRCR"
     gene            8124..9209
                     /locus_tag="IE198_RS27375"
                     /old_locus_tag="GCM10010381_55590"
     CDS             8124..9209
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459504.1"
                     /locus_tag="IE198_RS27375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010381_55590"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_189891247.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 7e-20, bitscore:
                     64.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDHTVLHVKGRVLVGPEEVRDEVWAVDGRITFERPRAEAVTVHG
                     WALPGLVDAHCHVGLDRHGPVDPATSEKQALTDREAGTLLIRDAGSPSDTRWIDDREDL
                     PKIIRAGRHIARTRRYIRNYAHEIEPEDLVAYVAQEARRGDGWVKLVGDWIDRDAGDLT
                     ACWPRGVVEAAIAEAHRLGARVTAHCFAEDSLRDLVESGIDCIEHATGLTEETVPLFAE
                     RGVAIVPTLVNIATFPSLAAGGESRFPAWSAHMRRLYERRYDTVRAAYDAGVPVFVGTD
                     AGGSLAHGLVAAEVAELVKAGIPPLQALSATTWGARSWLGRPALEEGAPADLVVYDEDP
                     RADVRVLAAPRRVILNGRVID"
     misc_feature    9416..9429
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.96"
                     /tool="antismash"
ORIGIN
        1 cactgctccc accggagctc cgcgacgaca acccgcacgc acttccacgg cacgtcgacg
       61 gccaactgca acactgaccg tcatggaact gaagctgccc gatctcccct tcccgctgcg
      121 ggcatacggt cccgaggccg actggtccta cgaggacggt gtgctgaccg cctgggccgg
      181 cccccgacag gatcgtttcg tcccgccgac cggccactcc ctcgaaccgg cctccgacgc
      241 gccccgtctg ctcggcgccc cggagggcga tttccagctg atcgcccgcg tcaaggtcgg
      301 cttcgcggcc gccttcgacg cgggcgtgct gtatctgcac gtcaccgaac aggagtgggc
      361 caagctctgc ctggagctct ccccggacag gcccaccgtg tgcacggtcg tcacccgcgg
      421 ccactcggac gacgccaact ccttcgtggt ggacggcgac agcgtctggc tgcggatcag
      481 ccgtaccgga aaggcgttcg ccttccacgc gtccacggac ggcgagcgct ggaccttcgt
      541 ccgcatcttc acgctcggcg acgaggaccg ggccggcgcg gcgctcgtcg gcttcatggc
      601 ccagtcgccg gtgggcgagg gctgcgtggt ctccttcgac cgcatcgagt accgcgacag
      661 ctggccggaa gggctgcgcg acggctcctg acgacacccg ccggccggcg gcgtcgcagg
      721 ccgggcccct cgggcgggtg gcgtcatgcc gacggcagga ccggtggacg ccccgtcgtc
      781 cagacggcct cgaccgacat ccgcctgaag cgccagccgt cgtccgtgcg ccgcagatct
      841 gcctcgtagc gcccgccgac ggtgaacagc gggtcggggc cctcgtggtg gaggtgcacc
      901 atcagggcgt tccatgacgc gcgggccgtt ccggacgcgg agtcgacgtc ggtcagcacg
      961 tcggaggcca tgtgctgtgt acgggcgaac cgcccgacgg cctcctcggt gccctgcacg
     1021 gccgccgggc cgtcgtacac gcccaccggg gcgatcatgc ggatgtcgcc ggtgaagtag
     1081 ccgtcggccc agccgtccgc gaacttccgt gcgtcaaggg cgcggaagag ccggctgacg
     1141 agcgtggtga tctcgatgtg atccgtctgt ggggtcatga gtacgagcgt tcaccctcaa
     1201 ctgcgcttga ggtcaaggac agttgttgcc gccgatgcgc acgggcccgc aaccgccgct
     1261 gcccttgcta cgtctcctcc cacatgatcc agatcgccgc cgccgccgac ctcgacgcca
     1321 tcgccgccct tcacgcggag gcccgcgcca cgtactactg gggccacatc ccggatgagg
     1381 ccttcgacag cccggccgag cacgcccgca cccggacggg ctgggagaac gcgatcgggc
     1441 gcggcgccgt cctgtgcgcg atacgcgacg gggccgtctg cggggtggcc gccttcggtg
     1501 atcgcaaggg cgtcatgacc ctttcgcagc tccacgtcgc tccggggcac tggcgctcgg
     1561 gcgtcggcag cgaactccac gccgcggtcg tcgcggcgtg gcagcgcgac caggtgacca
     1621 ccgcccgcct cgaggtcttc gagaagaacg agcgcgccca ggccttctac acccaccacg
     1681 gctggtaccc ggacccgcag gacccgcacg ccggcaatca cctgatcctc cgcttctcgg
     1741 tcccgtccgc ggcgtaggcg agggccacct ttggccgtca tgacctgcac tcgccgtacg
     1801 cgggggccgc ccaccaccgg ggatgtttgt ccgcgcagca ctggcggcgg gtgcgacgga
     1861 cgcgtacctc gccggtcccg gcaggggcgg aatgagccgg gggcggttcc tgttgagctc
     1921 tgtgcggagg cgccctccgt gcccccgccc ggtagccacc gccatggcac gaccctggag
     1981 tgatgaagaa gcatgcgcgt cgagatctgg tcggacatcg cctgtccctg gtgctacatc
     2041 ggaaaggccc gcttcgagaa gggactcgag gccttcgcgc accgcgacga gatcgaggtc
     2101 gtgcaccgtt ccttcgaact cgacccgaac cgcgcagggc acgacaccac cccggtcgtc
     2161 gacctgcttg cgaagaagta cggccgcacc cgcgaggagg cccgcgccat ggaggagcac
     2221 gtcgcctcca ccgcgcgctc cgaggggctg gagtaccgca ccgagggccg tgaccacggg
     2281 aacaccttcg acatccaccg gcttctgcac ctggccaagg cacgcggcct ccaggacgag
     2341 ctgctcaccc tcgtctatcg ggcgaacttc gccgaggagc ggtcggtctt cgatccggag
     2401 gtgctcgtcg cgctcgcggc ggaggccggg ctcgacgagc aggaggcgcg ccgggccctg
     2461 gccgacgagg ccgcttacgc ggacgacgtg cgggccgacg agcgtgaggc ggccgagctg
     2521 ggcgccaccg gggtgccgtt cttcgtgttc gaccggcggt acggggtctc cggcgggcag
     2581 cccgccgagc tgttcactca ggcgctggag caggcctggc agggccgctc ggtaacgccc
     2641 cccggcggcg cggccgcgtg cgacgccgac ggagcctgcg aggtcccggc cgccgccacc
     2701 gaagcaacgg tccactgatc ggcgcctctg aacggttccg tgcagcaagg cgtgattgac
     2761 ccggtgggcc gtgcatcgca gggtggtgcc atggagatct cgaagccgct gatcgacgcc
     2821 gagttcgcgc cgcgcacgac gtatctgaac acagcggtca acggcctgct gccccgccgg
     2881 tcggtccacg ccgtcaaggc gctcgcacaa gagctcggcg aggggaggcc gggtggctcc
     2941 ggcgactact cggccgtgac cgcggcccgc cggtccttcg cccgcctcac aggggtggac
     3001 gaggggcggg tcgccatcgg cggttcggtc tccgtccacg ccggactgat cgcggcctcg
     3061 ctccggtccg gtgcggaagt gctgctgccc gagggggagt tcacctccct cgtcacaccg
     3121 ttcgccgtcc gcggcgacct gaagctgcgc caggtcccgc tcgacgagct cgccgaggcg
     3181 gtccgccccg gcacggacct ggtcgctctc tcttccgtgc agtccgcgga cggcagggtc
     3241 gccgacctgg tggccgtacg ggcggcagcg gcggcccacg gggcccgtac gctcctggac
     3301 gccagccagt cggcgggctg gctgccactg gacgcggcag cctgggactt caccgtcacg
     3361 gccggctaca agttcctgct ctgcccgcgc ggcgtctcct tcctgaccgt gaccgaggag
     3421 gctcaggagt cgctgccgcc cgcgcacgcc gggacgttcg cggccgagcg gccggggcag
     3481 gccgacagca tgtacggtcc gatcaccgag ctggcccgct ccgcccgccg ctacgacgag
     3541 accccggcct atctgccgta ccacgcggcc gaacagtcgc tggcgctgct ggaggagatc
     3601 ggtatccaga ccgtgcacgc ccacgacacc gcgctggccg ggcggttcag cgccgggctc
     3661 aggcggcacg gcctcgaacc ggtggcgggc gagtcggcga tcgtggccgt ccccggcctc
     3721 ggtgaccggg cggaggaact gtgccgggcg gggatcctcg tctcgggacg cgcaggcaat
     3781 ctgcgcgtca ccttccacct ctacaactcg cccgcggacg tcgagcgggc gctggacttc
     3841 ctcgtgggct gagcgtgcgg aagggggagc ggatggacca ggggtgggcg gccgtcgtgg
     3901 ccggcgggcc gggctcatgg tccccgtcgt gggcgggttc ttcaccgtgc tgccgggcgg
     3961 ggtgcccgcc ccgctcaggg gcctgtgaag caaggccttt cggcatgacg gaccctttcg
     4021 gcatgacgga cgtgccgaag aggccacgcg taagaggccg ttctcccaga gaccggcccc
     4081 ttccgcgtgc caggacgggc tagcgcaccg gcgtgaagtc ccgcgccccg atgaactcgg
     4141 gccgcggcac cggcgccgcg aacggctcga ccgccgcgtt ctccacgctg ttgaagacga
     4201 tgaagacgtt gctgcgcgga tacggggtga tgttgtcacc cgagccgtgc atgcagttgc
     4261 agtcgaacca ggtcgcggac ccggccctgc cggtgaagag ccggatgccg tggtcgtcgg
     4321 cgaaacgcgt cagcgcctcg tcggacggcg tgccggcgtc ctgcatctgc agcgacttct
     4381 tgtagttgtc cttcggcgtc tcgcccgcgc agcccaggaa cgtcttgtgc gaccccggca
     4441 tgatcatcag accgccattg gtgtcgaagt tctgggtcag cgcgatcgag acggagacgg
     4501 tgcgcatgtt cggcagaccg tcctcggcgt gccacgtctc gaagtccgag tgccagtaga
     4561 agcccgaggc gccgaagccc ggcttcacat tgatacggga ctggtggacg tacacgtcgg
     4621 agccgaggat ctgacgtgct ctgcccacca cccgctcgtc gcgaaccagc gaggcgaaga
     4681 cctcgctgat cttgtggacc tcgaagaccg accgcacgtc ctgcgacttc ggctcgatga
     4741 tcgagcgctc gtcggcgcgc accatcgggt cgctgatcag ccgctccagc tcggagtggt
     4801 agcgagcgac ctcgtcgtca ccgatcagct ggtcgacggt gaggaagccg tccctctcga
     4861 agcccatcag gtcggccgtg tcgatcggcc ccagcgagcc gggcgcggac cagacgaccg
     4921 ggtccttgcg gggggtcagc acctcggagg cgccacgggt cgggtacagg tcggtgcgtg
     4981 cgggtgcggt ggtcatttgc tcagccctcc tcggtcagca gcgggtagac accgttctcg
     5041 tcatggtcct cccgtccggt gacgggggga ttgaagacgc agaccacgcg gaagtcggtc
     5101 ttgggtcgca gcgtgtgctt ctcgtgcccg tccagcaggt acatcgtgcc gggctcgatc
     5161 cagtgggtct cgcccgtctc gtcgttggtg agctcggcct cgccctcgac gcagagcacg
     5221 gcctcgatgt ggttcgcgta ccacatcgac gtctccgtgc ccgcgtacag cacggtctcg
     5281 tgcagcgaga agccgacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gaagcggact tcacatggcg ctcggtgttc tcgatgtcct tgaacgatcg gacgatcaca
     5401 gtggttcttt acctttcggt ggcaggtggt caggcggtct cgcggacggc gcgcgccacg
     5461 atgcgcagac cctggtccaa ctcgtcggag gagatggtca gcggcggcag cagcttcacg
     5521 acctcgctct ccggtcccga ggtctccagc agtagtccga gctcgaaggc gcgggcgcag
     5581 acggcggacg cacgcggctt gtcgtcgaac tccatgcccc agaccaggcc acggccgcgg
     5641 aagctcacgc cctcgccgcc catctcgtcg acgatcgcga gcagcgcgcg ctcgacctgc
     5701 tcaccgcggg cgatggtctg cttctccatc tggccgtcgg cccagtacgt gttgagcgcg
     5761 gcggcggccg tcacgaacgc cgggttgttg cctcggaagg tgccgttgtg ctcgcccggc
     5821 tcccagatgt ccagctccgg cttgaacagc gtgagcgcca gcggcaggcc gtagccgctg
     5881 atggacttgg agacggtgac gatgtccggc acgatgccgg cctcctcgaa ggagaagaag
     5941 ccaccggtgc ggccgcagcc catctggatg tcgtcgacga tcagcagcat gtcctggcgg
     6001 tggcacagct cctgcagcgc gcgcagccac tcggcgcggg ccacgttgat gccgccctcg
     6061 ccctggaccg tctcgacgat cacggcggcg ggcttgttga ggccggaacc ctggtcctcg
     6121 agcagccgct cgaaccagag gaagtccggc acctggccgt cgaagtagtt gtcgaacggc
     6181 atcggcgtgc cgtgcaccag cgggatgccg gcgccggccc gcttgaaggc gttgccggtg
     6241 acggcgagcg agccgagcga catgccgtgg aaggcgttgg tgaacgagac gatcgcctcg
     6301 cgccccttga ccttgcgggc cagcttcagc gccgcctcga cggcgttggt gcccgtcggg
     6361 cccgggaaca tgaccttgta cggcaggtca cgcggccgca ggatcacgtt ctggaacgac
     6421 tccaggaacg cacgtttggc ggtggtggcc atgtccaggc cgtgggtgat gccgtcacgc
     6481 tcgatgtagt cgatcagggc gcgtttcagc accgggttgt tgtgcccgta gttgagcgat
     6541 ccggcgccgg cgaagaagtc gaggtaggtg tggccgtcct cgtcgtggag gtggctgccc
     6601 tgcgcgcggt cgaacacggc gggccagccg cggcagtagc tgcgcacctc ggactcgagg
     6661 gtctcgaaga cgctcagggc gggcggggtg atggtcacag cgaactccag ggagtggggg
     6721 tcgggcgcga gaggtcaggg atcacacggc ccgtgcggca cggcgtgccg gcacgcggct
     6781 cgcggtacgc gtcacgcggc aacggggccg atgcggtaca gcacttccgg ctcgtgcgtt
     6841 ccctcgggga acagcccgcc gtcgaagagg acctcgcggc tgagggatgc tccatggcgc
     6901 ttggcgtagg aggtgaacag ggcgtcggag gcggtgttgt ccggggtgat ggtggtctcg
     6961 atctcgcgga ccccgtgtcc gccggtgacc ttggcggtca gtgcgtccag caggacgccg
     7021 gccagccccc ggccgcggtg cgcacggtcg acggccacct gccagacgac gagcgcctct
     7081 gggcggtcgg ggcgtacgta tccggtgacg aaggcgatgg gttcgccgtc acggccgcgg
     7141 gccacgacag atgtggctgc gaagtcgcga caccacagca ggtagctgta cgaggagttg
     7201 aggtccagga cctcggagtc gcgggcgatg cgccagatcg cggctccgtc ctccactcgt
     7261 ggggtgccga tctccaggaa ttcgtctcgg gctcgtacaa ggtcttcttg tgcggcggtc
     7321 atgtcgattc aatttaccga gcaaattccg agattgcatc gggccagggg gttgggtggc
     7381 gagagccgtt gtgttatcgc gcgggcgcga gctcgacgcc gatcaccgcg cgatcgcgcg
     7441 tatatgcccg aatatatccg ggcaaagcgc cctctctgtg gtgtcggtca catgtcggta
     7501 acccggttga cacatgatcg aaccgccccg cgaactgata tgaatccgca cgtttagccg
     7561 tggcgatcgg gggcagcaga atgcggggag ctatttactg gaatttgctc cccgtattta
     7621 tgtgcgatgc tctgggccgc tatgaaatgc gcgtttttcg aaaagcggtc gggaccagtc
     7681 acttccagct ctcgccggct gcccggtgtg cggcctcgag gtccaccgcc ctgccgtgcc
     7741 cggacaggtg caccgcccag gccgccggcg aggaccgcac gacgcccggg tcgcgtccac
     7801 gccgtagtgg tggacccggt ctccgcggcc acggcaccgc ccccggcgat caacggcagc
     7861 gacgggtcgg tggccaggcc ttcgccacca ggtcgcgggc gtccactccg gccggggcgc
     7921 gcggcgtcgt cgccaccggg gccgcgtccc gcgccgcgtg cgccggtgac cacgttcagc
     7981 gccgtcgaac cggggtgcgc gctgccgcgg atgcagccct ttaggggcag cagcgcctcg
     8041 ccctgcatga tgtttccagg cggtgttcgg acagtgttcg ggctctcgcc cgggactacc
     8101 gagggcctcg tacagttcct gacatgagcg atcacacggt gctgcatgtg aaggggcggg
     8161 tgctcgtcgg gcccgaggag gtgcgggacg aggtgtgggc ggtggacggc cggatcacct
     8221 tcgagcgtcc acgtgccgag gcggtcaccg tgcacggctg ggcgctgccc ggactggtcg
     8281 acgcccactg ccacgtcggt ctcgaccggc acggcccggt cgaccccgcg accagcgaga
     8341 agcaggctct caccgaccgg gaggcgggca cgctgctgat ccgggacgcg ggatcgccgt
     8401 ccgacacccg ctggatcgac gaccgtgagg atctgcccaa gatcatccgg gcgggccggc
     8461 acatcgcccg cacccgccgc tacatccgca actacgccca cgagatcgag cccgaggacc
     8521 tggtggccta cgtggcgcag gaggcccggc gcggcgacgg ctgggtcaag ctcgtcggcg
     8581 actggatcga ccgcgacgcc ggcgacctga ccgcctgctg gccgcgcggc gtcgtggagg
     8641 cggccatcgc ggaggcgcac cgcctcggcg cccgggtgac ggcgcactgc ttcgcggagg
     8701 attcgctgcg cgacctcgtc gagtccggca tcgactgcat cgagcacgcg accggcctca
     8761 ccgaggagac cgtcccgctc ttcgcggagc ggggcgtcgc catcgtcccg accctggtca
     8821 acatcgccac cttcccctcc ctcgcagcgg gcggagagtc caggttcccc gcctggtctg
     8881 cccatatgcg ccgcctgtac gagcgccgct acgacaccgt gcgcgcggcc tacgacgccg
     8941 gtgtcccggt cttcgtcggc acggacgcgg gcggctccct ggcgcacggg ctggtggccg
     9001 cggaggtcgc cgaactcgtg aaggccggca ttccgccgct ccaggcgctg tcggcgacca
     9061 cttggggggc caggagctgg ctggggcgcc cggcgctcga ggagggcgct ccggccgacc
     9121 tggtggtcta cgacgaggac ccgcgggccg atgtgcgtgt gctcgccgca ccgcgccggg
     9181 tgatcctgaa cgggcgggtc atcgactgac ggggcggcga tgccgccggg ttgaccgagc
     9241 gtgacccggc gcccgttccc gtcgttgaag cacttcacgc gcgccgcctc cggtgaccgg
     9301 cgcgccactc ctgaggccgt gacgggaggg gtgcagagga acgcctgtgc ctcggtaatc
     9361 gaatgcgagc tcagaaaccc cactttggag tgaactcgct tcaggtgtct gtcagttcac
     9421 tctccgtgcg taaagatttc ggtgtctggt cgccgccgtc cacgtgtccc catgggacgg
     9481 cgggcgacgc catgtctctg tgggggtttc accaccgtga acagcaacac cttccgcatg
     9541 cccgcacgca aggccgccgc cgtgacggcc gccgtcgcac tggccgctgt gcccgtggca
     9601 ctcgccgccc ccgcccacgc caccggcggc gagggcaggg cctccgcggt cgtgctccgc
     9661 accggcctgg acgtctccct gctcgacaag accgcgcagg tgccgctgag gaccaccctc
     9721 aacgaggtac gggcgcccgg gagcgccgag aagaccgcgc tgagcgtcga gttggacggc
     9781 gtcgacgggg acaggcccgt ccagcttctg cgcgccgacg tggccacggc cagggccacg
     9841 gtggaggacg gcagggccga ggcgcacagc aacctggtca acgccaggat ccatgtgccc
     9901 ggcctgccgc tgctgtcgct gatcgaggtg gagcaggtca cctccaaggc cgtctgcgag
     9961 gcaggcgccc ggcccgtcgc cgaatcgaac gtcctcggca gggtcaaggt cctcggcaag
    10021 aaggtcgagc tcagcgcggg cgggccgacc cgcgtcgagg tgccgggcgt gggcgaggtg
    10081 agcctcgagc tgtcgaggac gcacacgacc tcccgtacgg ccgccgcgac agcgctacag
    10141 ctcaaggtcg ccgtcgaccc tctgaagctg aacgtcgccg aggtcggggg cgaggtcacg
    10201 ctcgcggagg cgacctgcga aaccaccgac gcaccgaagg cgcccgtaaa gcccgccgag
    10261 cccgcacccc ccgcggacga ggagccggcc gggaagccgg ccgacgaagg catctccgcc
    10321 cagtccggtg aggagcccgc ccaggacaac ctcgcggaga ccggcgggag ttccacgacg
    10381 ccctacctcg ccggcggcg
//
