LOCUS       NZ_BMVW01000036        11482 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces poonensis strain JCM 4815 sequence36, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVW01000036
VERSION     NZ_BMVW01000036.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces poonensis
  ORGANISM  Streptomyces poonensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 11482)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 137X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650915.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:27:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,577
            CDSs (total)                      :: 7,501
            Genes (coding)                    :: 7,228
            CDSs (with protein)               :: 7,228
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 273
            CDSs (without protein)            :: 273
            Pseudo Genes (ambiguous residues) :: 0 of 273
            Pseudo Genes (frameshifted)       :: 96 of 273
            Pseudo Genes (incomplete)         :: 224 of 273
            Pseudo Genes (internal stop)      :: 19 of 273
            Pseudo Genes (multiple problems)  :: 60 of 273
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:46:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 11482
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVW01000036.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11482
                     /culture_collection="JCM:4815"
                     /db_xref="taxon:68255"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces poonensis"
                     /strain="JCM 4815"
                     /submitter_seqid="sequence36"
                     /type_material="type strain of Streptomyces poonensis"
     gene            <1..>11482
                     /locus_tag="IE242_RS37180"
     region          1..11482
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..11482
                     /SMILES="CC(O)CC(O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11482
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4:>11482](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5..>11482
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             5..>11482
                     /NRPS_PKS="Domain: PKS_KR (400-575). E-value: 7.7e-55.
                     Score: 177.6. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (664-750). E-value: 1.2e-32.
                     Score: 104.0. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (770-1196). E-value:
                     8.6e-188. Score: 616.4. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (1299-1575). E-value: 1.2e-104.
                     Score: 341.8. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (1901-2076). E-value: 2.6e-58.
                     Score: 188.9. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_PKS_KR.2"
                     /NRPS_PKS="Domain: ACP (2181-2253). E-value: 2.3e-30.
                     Score: 96.7. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (2274-2700). E-value:
                     9.6e-183. Score: 599.8. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_PKS_KS.2"
                     /NRPS_PKS="Domain: PKS_AT (2804-3091). E-value: 1e-111.
                     Score: 365.0. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_PKS_AT.2"
                     /NRPS_PKS="Domain: PKS_KR (3430-3605). E-value: 4.8e-58.
                     Score: 188.0. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_PKS_KR.3"
                     /NRPS_PKS="Domain: ACP (3713-3785). E-value: 2.9e-31.
                     Score: 99.6. Matches aSDomain:
                     nrpspksdomains_IE242_RS37180_ACP.2"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981853.1"
                     /locus_tag="IE242_RS37180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189867510.1"
                     /sec_met_domain="PP-binding (E-value: 1.7e-17, bitscore:
                     50.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 5.3e-238, bitscore:
                     775.9, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 5.4e-97, bitscore:
                     310.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.7e-113, bitscore:
                     365.0, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 5.1e-61, bitscore:
                     191.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 7.1e-11,
                     bitscore: 27.8, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEYWVEHVREAVRFADGIATLHELGVGTFVEIGPGGVLSALAQGC
                     LDGDADVVTVPVLRADRPEEQAVVTALADLHVHGHEVDWRAFFPGARRVDLPTYAFQRE
                     RYWLEAPEAEAEAAAVDAVDAEFWASVEREDAQSLAATLELSPGELDVVIPKLSAWRRR
                     RREQSLVDGWRYQLTWQPLNGLSAGDLTGTTWLFAAPEGDQWARTVHESLTGRGAYLVP
                     LATGVGADRASLAEELTSAVAAAGPVDGILSLLAVDETATPGQPALPQGLAHTVALAQA
                     LGDADVDAPLWCVTRGAAATGRSDDAPLSAVQNQVWGLGRALALEHSRRWGGLIDLPAA
                     VDERAAGRIAAVLRQGTASLGQGTGTGEGGEDQVAVRSSGVFTARLAHARSESGRPWTP
                     RGTVLVTGGTGALGGHIARWLAGAGAEHLVLAGRRGADASGAPALKAELEELGARVTLA
                     ACDVSDRDAVAALLAEHTFTSVFHAAGVPQFGAFDELAPADLARTLAAKAHGAAHLDDL
                     LGDRELDAFVLLSSIAGVWGSGLQTGYAAANAFLDGLAARRRARGLTATAIAWGPWADG
                     GMVTDSDEEHLRRRGVVTLPAALAVTAVQRALDCDDTAVVVADIAWDRFIGPFTLGRPS
                     ALLSELPEVRRAAPADAAEGTGATPLAARLAGLPEAERTQALADLVRAHVAAVLGHSSA
                     AEIEPDRAFKDLGFDSLTAVELRNEVNAATGLALPPTLVFDYPNATALARFLQSELLEA
                     QPAAPQAQHSAAADDEPIAIVAMSCHLPGGVDSPEALWDLVTSGGDAVSEFPSDRGWDL
                     DGLYDPDPERPGTAYARDGGFLHHAAEFDAGFFGISPREALAMDPQQRLLLETSWEAFE
                     RAGITPAQLRGSRTGVFVGMAYQGYGAEARRMPEGVEGHRLVGGASSVVSGRVAYTFGL
                     EGPAVTIDTACSSSLVALHLAMQSLRNGECTMALAGGVTVMASPTVFVEFSRQRGLSVD
                     GRCKAFAAGADGTGWAEGAGVVLVERLSDAVRNGHQVLAVVRGSAVNQDGASNGLTAPN
                     GPAQQRVIRQALASAGLTPGDVDAVEAHGTGTRLGDPIEAQALLATYGQERADGQPLWL
                     GSLKSNIGHTQAAAGVAGVIKMVMALRHGVLPRTLHVDEPTPHVDWSAGDVRLLTEAVD
                     WPETDRPRRAAVSSFGVSGTNAHTIIEQAPAAAAGPGAAPAPSAPVPWVLSGKGEAALR
                     AQAARLAGHLDGVGGWTAADIGYSLALRETFEDRAVLLAQEPDDLRHALTALAAGEPAA
                     NVTLGRARSGGRTGFLFSGQGSQRIGMGRELYEAFPVFADAYDEACARLEAPVDVDDEG
                     LHRTGAAQPALFAVEVALFRLLESWGVRPDYVAGHSVGEIAAAHVAGVLSLEDAAALVS
                     ARARLMQALPEGGAMVAVQASEEEVLPHLAEGVGVAAVNGPRSVVVSGAEAAVLAIGEV
                     FREQGRKTSRLKVSHAFHSPLMEPMLAEFGEVVAGLSFEQPRIPVVSNLTGRLVESYTP
                     EYWVEHVREAVRFADGIATLHELGVTTFVEIGPGGVLSALAQGCLDDAVAVPVLRGDRP
                     ERQAVLGALAELHVHGVSPHWQALFPGARRVVLPTYAFQRERYWLEGDEGPAAAGTPAD
                     AADSGFWDSVEREDAESLAAALGVDADASLSAVLPRLSAWRRHRREQSVVDGWRYRVTW
                     KPLGALPRSGASGTWLLVAAAESEWTGSVRTALVDRGLDLVTLVAGPGTDRGALARELA
                     GAGPVTGVLSLLAEDDSASGTDPALTNGLAATLRLVQALGDAGIDAPLWCATRGAVATG
                     RSDRVDRPLQSQVWGFGRAAALEHPQRWGGLLDLPDVLDARTAARVVAVLGQTAEDQVA
                     VRASGAFGRRLVRAARATGPARGWSPRGTVLITGGTGALGGHVARWLAGAGAEHLVLAG
                     RRGLDAPGAAALRAELEETGVDVTVAACDVADRDAVAALLAVHPVDAVVHAAGASDHAM
                     IEDSDEAGFAGTVAAKTAGATHLHELLADRDLDAFVMFSSGAGIWGGAGQSAYAAANTY
                     LDALAEYRRARGRAALAVSWGGWAEGGMAGVGDGEEMLRRRGLPPMRPALAISALQQAV
                     ADDETLLTVADFDWQRFIGPFTVTRPSALFSDMPEARGALEAPASGGAEAGNALAAKLA
                     VLSAGEQDRTLVDLVRAHAAVVLGHDGAAAVEPGRAFNDLGFDSLTAVELRNRLTADTG
                     LKLPTTLVFDHPDATALARFLRTELLGAQEAAPQELHPAATGGDEPIAIVAMSCRLPGG
                     VGSPEDLWQLVTSGGDAITAFPEDRGWDVEGLYDPDPGAPGKTYTLAGGFLHEAGDFDA
                     AVFGVSPREAIAMDPQQRLLLEITWEAFERAGIAPASLKGSRTGVFVGMSYQGYGAGLP
                     EVPEGVEGHLLTGSAASVVSGRVAYSFGLEGPAVTVDTACSSSLVALHLAMQSLRSGES
                     TMAVAGGVNVMALPAAFVEFSRQRGLSVDGRCKAFGAGADGTGWAEGAGVVLVERLSDA
                     VRNGHQVLAVVRGSAVNQDGASNGLTAPNGPAQQRVIRQALASAGLGEADVDAVEAHGT
                     GTRLGDPIEAQALLATYGQQRPEGRPLWLGSLKSNIGHAQAASGVAGVIKMVMALRHGV
                     LPRTLHAEEPTPHVDWSAGDVRLLTEAVDWPETDRPRRAAVSSFGVSGTNAHTIIEQAP
                     VAEPAPVAPVEVASATVPWVLSGKSEAALRAQAERLLVRTADGTGPSLTDIGFSLATTR
                     TALEHRAAVVGDDHGRLTEGLRALAEGRPAPGAYLGRADTGQVGFLFSGQGSQHAGMGR
                     ELYEAFPVFAEAYDEVCAHLDRHLDQPLRAVAFGEDGHEDGEHLNRTAFTQPALFAHEV
                     AVHRLLESWGVRPDYLAGHSVGEIAAAHVAGALTLEDAATLVSARARLMQALPEGGAMI
                     AVEATEDEVLPHLTEGAGIAAVNGPRSVVLSGAEDAVAAVAAHFAQEGRRTSRLAVSHA
                     FHSPLMDPVLEEFARVAAGLRFEPPRTPVVSNLTGRLIDSYTADYWVRHVREAVRFADG
                     IATLGELGVTTFVETGPGTVLSGMARTCLDDAVTVPTTRSGSPGPETITDALARLHVHG
                     VPVDWHAYFAGRGARRTDLPTYAFQHQRYWLTTTAPTAAATGADAVEAGFWETVEREDA
                     QSLAATLDLPAGQLDAVLPRLSAWRRRQRRESAAEGWSYRTAWKPLGGLRTGRLTGDWL
                     LLSTEDPGDPEATGWTAAVADGLTALGARLVRVAVAPDADRGALRRQLTDAAGRTPLEG
                     VLSLLGTDERPYSGHPALRVGTALTLALVQALGDAGIDAPLWALTRSAMSTGRADTVPS
                     AAQNAVWGLGRVAALEHADRWGGLIDLPGTIDGRIVGRLAAVLGQRDEDQVAIRHRGVF
                     GRRLAHVTARRDTPAGQRWSPRGTVLITGGTGALGGHVARWLAEAGAEHLVLTSRRGPD
                     APGAADLVAELETAGARVTVAACDAADREALAGLLAEHPVDAVVHAAGVDHLDPLDAMT
                     PGAFADALSAKAAGALHLAELLHDRELDAFVLFSSIAGVWGSGHQAAYAAANALLDGVA
                     ERRRAQGLPATAVAWGPWADGGMAEGDGADERLRRRGLIPMPAALALVGLRQALDSGEA
                     TTTLADVDWGRFLPPFTMGRPSALLGDLPEAEQIRTAGGSSGGPGTAADSPLAGRITAL
                     PENEQHTLLVDLVRTHAAAVLGHSGIGEVEADRAFKELGFDSLTAVELRNRLNDETGLT
                     LSATLVFDHPNAQALARQLRTELTGRTGVPAPEAAAGPAEDDEPIAIVGMACRYPGGVR
                     SPEDL"
     CDS_motif       13..48
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0001"
                     /evalue="2.90E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IE242_RS37180"
                     /protein_end="14"
                     /protein_start="2"
                     /score="14.2"
                     /tool="antismash"
                     /translation="YWVEHVREAVRF"
     CDS_motif       91..120
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0002"
                     /evalue="8.50E-01"
                     /label="PKSI-AT-M_m9"
                     /locus_tag="IE242_RS37180"
                     /protein_end="38"
                     /protein_start="28"
                     /score="3.5"
                     /tool="antismash"
                     /translation="FVEIGPGGVL"
     aSDomain        1207..1731
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_PKS_KR.1"
                     /evalue="7.70E-55"
                     /label="IE242_RS37180_PKS_KR.1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="575"
                     /protein_start="400"
                     /score="177.6"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: A1"
                     /tool="antismash"
                     /translation="GTVLVTGGTGALGGHIARWLAGAGAEHLVLAGRRGADASGAPALK
                     AELEELGARVTLAACDVSDRDAVAALLAEHTFTSVFHAAGVPQFGAFDELAPADLARTL
                     AAKAHGAAHLDDLLGDRELDAFVLLSSIAGVWGSGLQTGYAAANAFLDGLAARRRARGL
                     TATAIAWGPWAD"
     aSModule        1207..2256
                     /domains="nrpspksdomains_IE242_RS37180_PKS_KR.1"
                     /domains="nrpspksdomains_IE242_RS37180_PKS_PP.1"
                     /incomplete
                     /locus_tags="IE242_RS37180"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1210..1308
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0003"
                     /evalue="4.80E-12"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="434"
                     /protein_start="401"
                     /score="38.4"
                     /tool="antismash"
                     /translation="TVLVTGGTGALGGHIARWLAGAGAEHLVLAGRR"
     CDS_motif       1573..1683
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0004"
                     /evalue="9.70E-17"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE242_RS37180"
                     /protein_end="559"
                     /protein_start="522"
                     /score="53.5"
                     /tool="antismash"
                     /translation="LDAFVLLSSIAGVWGSGLQTGYAAANAFLDGLAARRR"
     misc_feature    1631..1633
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        1999..2256
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_PKS_PP.1"
                     /evalue="1.20E-32"
                     /label="IE242_RS37180_PKS_PP.1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="750"
                     /protein_start="664"
                     /score="104.0"
                     /tool="antismash"
                     /translation="LAGLPEAERTQALADLVRAHVAAVLGHSSAAEIEPDRAFKDLGFD
                     SLTAVELRNEVNAATGLALPPTLVFDYPNATALARFLQSEL"
     aSDomain        2317..3594
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="8.60E-188"
                     /label="IE242_RS37180_PKS_KS.1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1196"
                     /protein_start="770"
                     /score="616.4"
                     /tool="antismash"
                     /translation="IAIVAMSCHLPGGVDSPEALWDLVTSGGDAVSEFPSDRGWDLDGL
                     YDPDPERPGTAYARDGGFLHHAAEFDAGFFGISPREALAMDPQQRLLLETSWEAFERAG
                     ITPAQLRGSRTGVFVGMAYQGYGAEARRMPEGVEGHRLVGGASSVVSGRVAYTFGLEGP
                     AVTIDTACSSSLVALHLAMQSLRNGECTMALAGGVTVMASPTVFVEFSRQRGLSVDGRC
                     KAFAAGADGTGWAEGAGVVLVERLSDAVRNGHQVLAVVRGSAVNQDGASNGLTAPNGPA
                     QQRVIRQALASAGLTPGDVDAVEAHGTGTRLGDPIEAQALLATYGQERADGQPLWLGSL
                     KSNIGHTQAAAGVAGVIKMVMALRHGVLPRTLHVDEPTPHVDWSAGDVRLLTEAVDWPE
                     TDRPRRAAVSSFGVSGTNAHTIIEQAP"
     aSModule        2317..6765
                     /complete
                     /domains="nrpspksdomains_IE242_RS37180_PKS_KS.1"
                     /domains="nrpspksdomains_IE242_RS37180_PKS_AT.1"
                     /domains="nrpspksdomains_IE242_RS37180_PKS_KR.2"
                     /domains="nrpspksdomains_IE242_RS37180_ACP.1"
                     /locus_tags="IE242_RS37180"
                     /monomer_pairings="mal -> ohmal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       2572..2598
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0005"
                     /evalue="7.30E-02"
                     /label="PKSI-KS_m2"
                     /locus_tag="IE242_RS37180"
                     /protein_end="864"
                     /protein_start="855"
                     /score="6.5"
                     /tool="antismash"
                     /translation="DPQQRLLLE"
     CDS_motif       2800..2847
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0006"
                     /evalue="2.30E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE242_RS37180"
                     /protein_end="947"
                     /protein_start="931"
                     /score="19.5"
                     /tool="antismash"
                     /translation="GPAVTIDTACSSSLVA"
     CDS_motif       3223..3267
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0007"
                     /evalue="1.10E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1087"
                     /protein_start="1072"
                     /score="11.5"
                     /tool="antismash"
                     /translation="VEAHGTGTRLGDPIE"
     CDS_motif       3328..3372
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0008"
                     /evalue="5.50E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1122"
                     /protein_start="1107"
                     /score="9.6"
                     /tool="antismash"
                     /translation="GSLKSNIGHTQAAAG"
     aSDomain        3904..4731
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_PKS_AT.1"
                     /evalue="1.20E-104"
                     /label="IE242_RS37180_PKS_AT.1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1575"
                     /protein_start="1299"
                     /score="341.8"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFSGQGSQRIGMGRELYEAFPVFADAYDEACARLEAPVDVDDEGL
                     HRTGAAQPALFAVEVALFRLLESWGVRPDYVAGHSVGEIAAAHVAGVLSLEDAAALVSA
                     RARLMQALPEGGAMVAVQASEEEVLPHLAEGVGVAAVNGPRSVVVSGAEAAVLAIGEVF
                     REQGRKTSRLKVSHAFHSPLMEPMLAEFGEVVAGLSFEQPRIPVVSNLTGRLVESYTPE
                     YWVEHVREAVRFADGIATLHELGVTTFVEIGPGGVLSALAQGCLDDAVAVPVLR"
     CDS_motif       3907..3954
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0009"
                     /evalue="2.10E-05"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1316"
                     /protein_start="1300"
                     /score="17.1"
                     /tool="antismash"
                     /translation="FSGQGSQRIGMGRELY"
     CDS_motif       4048..4101
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0010"
                     /evalue="2.00E-03"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1365"
                     /protein_start="1347"
                     /score="11.5"
                     /tool="antismash"
                     /translation="GAAQPALFAVEVALFRLL"
     CDS_motif       4111..4233
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0011"
                     /evalue="5.20E-23"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1409"
                     /protein_start="1368"
                     /score="73.3"
                     /tool="antismash"
                     /translation="GVRPDYVAGHSVGEIAAAHVAGVLSLEDAAALVSARARLMQ"
     CDS_motif       4318..4356
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0012"
                     /evalue="4.20E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1450"
                     /protein_start="1437"
                     /score="13.6"
                     /tool="antismash"
                     /translation="AAVNGPRSVVVSG"
     CDS_motif       4420..4446
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0013"
                     /evalue="5.10E-02"
                     /label="PKSI-AT-M_m6"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1480"
                     /protein_start="1471"
                     /score="7.0"
                     /tool="antismash"
                     /translation="LKVSHAFHS"
     CDS_motif       4510..4542
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0014"
                     /evalue="2.20E-02"
                     /label="PKSI-AT-M_m7"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1512"
                     /protein_start="1501"
                     /score="7.8"
                     /tool="antismash"
                     /translation="PRIPVVSNLTG"
     CDS_motif       4570..4605
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0015"
                     /evalue="2.90E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1533"
                     /protein_start="1521"
                     /score="14.2"
                     /tool="antismash"
                     /translation="YWVEHVREAVRF"
     CDS_motif       4648..4677
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0016"
                     /evalue="8.50E-01"
                     /label="PKSI-AT-M_m9"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1557"
                     /protein_start="1547"
                     /score="3.5"
                     /tool="antismash"
                     /translation="FVEIGPGGVL"
     aSDomain        5710..6234
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_PKS_KR.2"
                     /evalue="2.60E-58"
                     /label="IE242_RS37180_PKS_KR.2"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2076"
                     /protein_start="1901"
                     /score="188.9"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: A1"
                     /tool="antismash"
                     /translation="GTVLITGGTGALGGHVARWLAGAGAEHLVLAGRRGLDAPGAAALR
                     AELEETGVDVTVAACDVADRDAVAALLAVHPVDAVVHAAGASDHAMIEDSDEAGFAGTV
                     AAKTAGATHLHELLADRDLDAFVMFSSGAGIWGGAGQSAYAAANTYLDALAEYRRARGR
                     AALAVSWGGWAE"
     CDS_motif       5713..5811
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0017"
                     /evalue="4.20E-12"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="1935"
                     /protein_start="1902"
                     /score="38.6"
                     /tool="antismash"
                     /translation="TVLITGGTGALGGHVARWLAGAGAEHLVLAGRR"
     CDS_motif       6076..6186
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0018"
                     /evalue="2.90E-21"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2060"
                     /protein_start="2023"
                     /score="68.1"
                     /tool="antismash"
                     /translation="LDAFVMFSSGAGIWGGAGQSAYAAANTYLDALAEYRR"
     aSDomain        6550..6765
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_ACP.1"
                     /evalue="2.30E-30"
                     /label="IE242_RS37180_ACP.1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2253"
                     /protein_start="2181"
                     /score="96.7"
                     /tool="antismash"
                     /translation="DLVRAHAAVVLGHDGAAAVEPGRAFNDLGFDSLTAVELRNRLTAD
                     TGLKLPTTLVFDHPDATALARFLRTEL"
     aSDomain        6829..8106
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_PKS_KS.2"
                     /domain_subtypes="Modular-KS"
                     /evalue="9.60E-183"
                     /label="IE242_RS37180_PKS_KS.2"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2700"
                     /protein_start="2274"
                     /score="599.8"
                     /tool="antismash"
                     /translation="IAIVAMSCRLPGGVGSPEDLWQLVTSGGDAITAFPEDRGWDVEGL
                     YDPDPGAPGKTYTLAGGFLHEAGDFDAAVFGVSPREAIAMDPQQRLLLEITWEAFERAG
                     IAPASLKGSRTGVFVGMSYQGYGAGLPEVPEGVEGHLLTGSAASVVSGRVAYSFGLEGP
                     AVTVDTACSSSLVALHLAMQSLRSGESTMAVAGGVNVMALPAAFVEFSRQRGLSVDGRC
                     KAFGAGADGTGWAEGAGVVLVERLSDAVRNGHQVLAVVRGSAVNQDGASNGLTAPNGPA
                     QQRVIRQALASAGLGEADVDAVEAHGTGTRLGDPIEAQALLATYGQQRPEGRPLWLGSL
                     KSNIGHAQAASGVAGVIKMVMALRHGVLPRTLHAEEPTPHVDWSAGDVRLLTEAVDWPE
                     TDRPRRAAVSSFGVSGTNAHTIIEQAP"
     aSModule        6829..11361
                     /complete
                     /domains="nrpspksdomains_IE242_RS37180_PKS_KS.2"
                     /domains="nrpspksdomains_IE242_RS37180_PKS_AT.2"
                     /domains="nrpspksdomains_IE242_RS37180_PKS_KR.3"
                     /domains="nrpspksdomains_IE242_RS37180_ACP.2"
                     /locus_tags="IE242_RS37180"
                     /monomer_pairings="mal -> ohmal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       7084..7110
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0019"
                     /evalue="7.30E-02"
                     /label="PKSI-KS_m2"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2368"
                     /protein_start="2359"
                     /score="6.5"
                     /tool="antismash"
                     /translation="DPQQRLLLE"
     CDS_motif       7312..7359
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0020"
                     /evalue="2.10E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2451"
                     /protein_start="2435"
                     /score="19.6"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       7735..7779
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0021"
                     /evalue="1.10E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2591"
                     /protein_start="2576"
                     /score="11.5"
                     /tool="antismash"
                     /translation="VEAHGTGTRLGDPIE"
     CDS_motif       7840..7884
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0022"
                     /evalue="1.10E-02"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2626"
                     /protein_start="2611"
                     /score="8.8"
                     /tool="antismash"
                     /translation="GSLKSNIGHAQAASG"
     aSDomain        8419..9279
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_PKS_AT.2"
                     /evalue="1.00E-111"
                     /label="IE242_RS37180_PKS_AT.2"
                     /locus_tag="IE242_RS37180"
                     /protein_end="3091"
                     /protein_start="2804"
                     /score="365.0"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFSGQGSQHAGMGRELYEAFPVFAEAYDEVCAHLDRHLDQPLRAV
                     AFGEDGHEDGEHLNRTAFTQPALFAHEVAVHRLLESWGVRPDYLAGHSVGEIAAAHVAG
                     ALTLEDAATLVSARARLMQALPEGGAMIAVEATEDEVLPHLTEGAGIAAVNGPRSVVLS
                     GAEDAVAAVAAHFAQEGRRTSRLAVSHAFHSPLMDPVLEEFARVAAGLRFEPPRTPVVS
                     NLTGRLIDSYTADYWVRHVREAVRFADGIATLGELGVTTFVETGPGTVLSGMARTCLDD
                     AVTVPT"
     CDS_motif       8422..8469
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0023"
                     /evalue="1.40E-05"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2821"
                     /protein_start="2805"
                     /score="17.6"
                     /tool="antismash"
                     /translation="FSGQGSQHAGMGRELY"
     CDS_motif       8596..8655
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0024"
                     /evalue="7.90E-02"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2883"
                     /protein_start="2863"
                     /score="6.7"
                     /tool="antismash"
                     /translation="RTAFTQPALFAHEVAVHRLL"
     CDS_motif       8665..8787
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0025"
                     /evalue="6.10E-22"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2927"
                     /protein_start="2886"
                     /score="69.9"
                     /tool="antismash"
                     /translation="GVRPDYLAGHSVGEIAAAHVAGALTLEDAATLVSARARLMQ"
     CDS_motif       8872..8910
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0026"
                     /evalue="8.20E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2968"
                     /protein_start="2955"
                     /score="12.7"
                     /tool="antismash"
                     /translation="AAVNGPRSVVLSG"
     CDS_motif       8974..9000
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0027"
                     /evalue="1.60E-01"
                     /label="PKSI-AT-M_m6"
                     /locus_tag="IE242_RS37180"
                     /protein_end="2998"
                     /protein_start="2989"
                     /score="5.5"
                     /tool="antismash"
                     /translation="LAVSHAFHS"
     CDS_motif       9064..9096
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0028"
                     /evalue="1.20E-01"
                     /label="PKSI-AT-M_m7"
                     /locus_tag="IE242_RS37180"
                     /protein_end="3030"
                     /protein_start="3019"
                     /score="5.8"
                     /tool="antismash"
                     /translation="PRTPVVSNLTG"
     CDS_motif       9124..9159
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0029"
                     /evalue="1.70E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IE242_RS37180"
                     /protein_end="3051"
                     /protein_start="3039"
                     /score="15.0"
                     /tool="antismash"
                     /translation="YWVRHVREAVRF"
     CDS_motif       9202..9231
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0030"
                     /evalue="4.10E+00"
                     /label="PKSI-AT-M_m9"
                     /locus_tag="IE242_RS37180"
                     /protein_end="3075"
                     /protein_start="3065"
                     /score="1.5"
                     /tool="antismash"
                     /translation="FVETGPGTVL"
     aSDomain        10297..10821
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_PKS_KR.3"
                     /evalue="4.80E-58"
                     /label="IE242_RS37180_PKS_KR.3"
                     /locus_tag="IE242_RS37180"
                     /protein_end="3605"
                     /protein_start="3430"
                     /score="188.0"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: A1"
                     /tool="antismash"
                     /translation="GTVLITGGTGALGGHVARWLAEAGAEHLVLTSRRGPDAPGAADLV
                     AELETAGARVTVAACDAADREALAGLLAEHPVDAVVHAAGVDHLDPLDAMTPGAFADAL
                     SAKAAGALHLAELLHDRELDAFVLFSSIAGVWGSGHQAAYAAANALLDGVAERRRAQGL
                     PATAVAWGPWAD"
     CDS_motif       10300..10398
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0031"
                     /evalue="6.60E-15"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE242_RS37180"
                     /protein_end="3464"
                     /protein_start="3431"
                     /score="47.6"
                     /tool="antismash"
                     /translation="TVLITGGTGALGGHVARWLAEAGAEHLVLTSRR"
     CDS_motif       10663..10773
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE242_RS37180_0032"
                     /evalue="1.00E-16"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE242_RS37180"
                     /protein_end="3589"
                     /protein_start="3552"
                     /score="53.5"
                     /tool="antismash"
                     /translation="LDAFVLFSSIAGVWGSGHQAAYAAANALLDGVAERRR"
     aSDomain        11146..11361
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS37180_ACP.2"
                     /evalue="2.90E-31"
                     /label="IE242_RS37180_ACP.2"
                     /locus_tag="IE242_RS37180"
                     /protein_end="3785"
                     /protein_start="3713"
                     /score="99.6"
                     /tool="antismash"
                     /translation="DLVRTHAAAVLGHSGIGEVEADRAFKELGFDSLTAVELRNRLNDE
                     TGLTLSATLVFDHPNAQALARQLRTEL"
     misc_feature    11447..11449
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 cgccggagta ctgggtggag cacgtccgcg aggcggtgcg gttcgccgac ggcatcgcga
       61 cgctgcacga gctgggtgtg ggcacgttcg tggagatcgg gccggggggt gtcctgagcg
      121 ccctcgccca gggctgcctc gacggcgacg cggacgtcgt cacggtgccc gtcctgcggg
      181 ccgaccgccc cgaggagcag gccgtcgtca ccgccctcgc cgacctgcac gtgcacgggc
      241 acgaagtgga ctggcgcgcc ttcttcccgg gtgcccgccg cgtcgacctg ccgacctacg
      301 ccttccagcg cgagcgctac tggctggagg ctcccgaggc cgaggcagag gccgcagccg
      361 tggacgcggt cgacgccgag ttctgggcga gcgtcgagcg ggaggacgcc cagtccctcg
      421 ccgccacgct ggagcttagc cccggggaac tcgacgtcgt catccccaag ctctccgcct
      481 ggcgccgccg acggcgcgag cagtccctcg tggacggctg gcgctaccag ctgacgtggc
      541 agccgctgaa cggcctctcc gccggtgacc tcacgggcac cacctggctg ttcgccgccc
      601 ccgagggcga ccagtgggcc cggacggtcc acgagtccct caccggacgc ggcgcgtacc
      661 tggtcccgct cgcgaccggc gtgggcgccg accgcgcgtc cctggccgag gagctgacct
      721 cagccgtggc cgccgccgga ccggtcgacg gcatcctgtc gctgctcgcc gtcgacgaga
      781 ccgccacgcc cgggcagccc gcgttgccgc aggggctcgc gcacaccgtg gccctggccc
      841 aggcgctggg tgacgcggac gtcgacgcac cgctgtggtg cgtcacccgg ggcgcggccg
      901 ccaccggccg atccgacgac gcgccgctga gcgccgtgca gaaccaggtc tgggggctgg
      961 gtcgcgccct ggccctggaa cactcccggc gctggggcgg cctgatcgat ctgcccgcgg
     1021 ccgtcgacga gcgcgccgcc ggccgtatcg ccgccgtact gcgacaggga accgcctcgc
     1081 tcggccaggg gaccggcacc ggggagggcg gggaggacca ggtcgccgtc cgctcctccg
     1141 gcgtgttcac cgcccggctc gcccacgccc gcagcgagag cggccggccg tggacgccac
     1201 gcggcaccgt cctggtcacc ggcggtacgg gcgcgctggg cgggcacatc gcccgctggc
     1261 tcgccggtgc cggcgccgag cacctggtgc tcgccggccg ccgcggcgcc gacgcgtccg
     1321 gcgcgcccgc gctcaaggcc gaactggagg agctgggcgc gcgcgtcacc ctcgccgcct
     1381 gcgacgtctc cgaccgcgac gcggtggcgg cgctgctcgc cgagcacacc ttcacctccg
     1441 tcttccacgc cgcgggcgtg ccccagttcg gcgccttcga cgagctggcc ccggccgatc
     1501 tcgcccgcac cctggcggcg aaggcccatg gcgccgcgca cctggacgac ctgctcggcg
     1561 accgtgagct ggacgccttc gtcctcttgt cctcgatcgc cggcgtctgg ggcagcgggc
     1621 tgcagaccgg ttacgcggcc gccaacgcct tcctcgacgg gctcgccgcc cggcgcaggg
     1681 cccggggcct gaccgccacc gccatcgcct ggggcccctg ggccgacggc ggcatggtca
     1741 ccgactcgga cgaggagcac ctgcgccgcc gtggggtcgt caccctgccc gcagccctcg
     1801 ccgtgaccgc cgtccagcgc gccctggact gcgacgacac cgcggtcgtc gtggccgaca
     1861 tcgcgtggga ccggttcatc ggaccgttca ccctcggccg tcccagcgcc ctgctgtccg
     1921 agctgcccga ggtgcgccgc gccgccccgg ccgacgccgc cgagggaacc ggcgccacgc
     1981 ccctggcggc ccggctcgcc ggactgccgg aggccgaacg cacccaggcc ctggccgacc
     2041 tggtccgcgc gcacgtcgcg gccgtgctcg gccactcctc ggcggcggag atcgagcccg
     2101 accgggcctt caaggacctc ggcttcgatt ccctgaccgc cgtcgaactc cgcaacgagg
     2161 tcaacgcggc gaccggcctc gcactgccgc ccaccctggt cttcgactac cccaacgcca
     2221 cggcgctcgc ccgcttcctg cagagcgaac tgctcgaggc gcagcccgcc gcaccacagg
     2281 cgcagcattc ggccgccgcc gacgacgagc ccatcgccat cgtcgccatg agctgccacc
     2341 tgcccggcgg cgtcgactcg ccggaggccc tgtgggatct ggtgacgtcc ggcggcgacg
     2401 ccgtctcgga gttcccctcc gaccgcggct gggacctcga cgggctctac gaccccgacc
     2461 ccgagcggcc cggcacggcg tacgcgcgcg acggcgggtt cctccaccac gccgccgagt
     2521 tcgacgcggg tttcttcggc atctcgccgc gtgaggcgct ggcgatggac ccgcagcagc
     2581 ggctgctgct ggaaacctcg tgggaggcgt tcgagcgggc cgggatcacc cccgcacagc
     2641 tgcgcggcag ccggaccggt gtcttcgtcg gcatggccta ccagggctac ggggccgagg
     2701 cgcgccggat gccggaaggc gtcgaggggc atcggctcgt cggcggcgcg tccagcgtcg
     2761 tctccggccg ggtggcctac accttcggcc tggagggccc cgccgtcacg atcgacacgg
     2821 cctgctcctc ctcgctcgtc gctctgcacc tggccatgca gtcgctgcgc aacggcgagt
     2881 gcacgatggc gctggcgggc ggtgtcaccg tcatggccag ccccacggtc ttcgtcgagt
     2941 tcagccggca gcgcggactt tccgtcgacg ggcggtgcaa ggcgttcgcc gccggtgccg
     3001 acggcaccgg ctgggcggag ggcgcgggcg tggtgctcgt ggagcgcctc agcgacgccg
     3061 tgcgcaacgg ccaccaggtg ctggcggtcg tgcggggcag cgcggtgaat caggacggtg
     3121 cgtcgaacgg tctgacggct cccaacggtc ccgcgcagca gcgggtgatc cggcaggcgc
     3181 tggcctcggc cgggctcacg ccgggcgacg tggacgccgt cgaggcgcac ggcaccggta
     3241 cgaggctggg tgacccgatc gaggcgcagg cgctgctggc cacgtacggt caggagcggg
     3301 cggacgggca gccgctgtgg ctggggtcgc tgaagtccaa catcggccac acgcaggccg
     3361 ccgcgggtgt cgccggtgtg atcaagatgg tgatggcgtt gcggcacggt gtgctgccgc
     3421 ggacgctcca tgtggacgag ccgaccccgc acgtggactg gtcggcgggt gacgtgcggc
     3481 tgctgacgga ggccgtcgac tggccggaga cggaccgccc gcgccgggca gccgtctcct
     3541 ccttcggcgt cagcggcacc aacgcccaca ccatcatcga gcaggccccc gccgccgcgg
     3601 ccgggccggg cgcggcaccc gccccgagcg cgccggtgcc ctgggtgctg tcgggcaagg
     3661 gcgaggcggc actgcgcgcc caggcggccc ggctggccgg ccacctcgac ggcgtgggcg
     3721 gctggacggc cgccgacatc ggctactccc tggccctgcg cgagacgttc gaggaccgtg
     3781 cggtgctcct ggcgcaggag ccggacgacc tccggcacgc tctcaccgcc ctcgccgccg
     3841 gtgaaccggc cgccaacgtg acgctgggac gcgcccgctc cggcggcagg accggtttcc
     3901 tgttctcggg tcaggggtcg cagcggatcg gaatggggcg tgagctgtac gaggcgttcc
     3961 ccgtcttcgc cgacgcgtac gacgaggcgt gcgcgcgtct ggaggcgccg gtcgacgtcg
     4021 atgacgaggg tctgcaccgg acgggggcgg cgcagccggc gttgttcgcg gtggaggtgg
     4081 cgctgttccg gctgctggag tcgtggggcg tccgcccgga ctatgtggcc ggtcattcgg
     4141 tgggtgagat cgcggccgcg catgtggcgg gggtgttgtc gctggaggat gccgcggcgc
     4201 tggtgtccgc ccgtgcgcgg ctgatgcagg cgctgccgga gggcggggcg atggttgcgg
     4261 tccaggcatc cgaggaggag gtgctgccgc atctggccga gggtgtgggt gtcgcggcgg
     4321 tcaacgggcc gcggtcggtg gtggtctcgg gtgccgaggc ggcggtcctc gccatcggtg
     4381 aggtgttccg ggagcagggc cgcaagacgt cccggctgaa ggtgagtcat gcgttccact
     4441 cgccgttgat ggagccgatg ctggcggagt tcggtgaggt ggtcgccggt ctgtcgttcg
     4501 agcagccgcg gattccggtg gtgtccaacc tcaccgggcg gctggtcgag tcgtacacgc
     4561 cggagtactg ggtggagcac gtccgggagg ctgtgcggtt cgccgacggc atcgcgacgc
     4621 tgcacgagct gggcgtgacc acgttcgtgg agatcggccc cggcggcgtc ctcagcgccc
     4681 tcgcccaggg ctgcctcgac gacgcggtcg ccgtgcccgt gctgcgcggc gaccgccccg
     4741 agcggcaggc cgtcctcggc gcactcgccg aactccacgt gcacggcgtg tccccgcact
     4801 ggcaggcgct gttccccggc gcccggcggg tcgtcctgcc gacctacgcc ttccagcgcg
     4861 agcgctactg gctggaaggt gacgagggcc cggcggccgc cggcaccccg gccgacgcgg
     4921 cggactccgg tttctgggac agcgtggagc gcgaggacgc ggagtccctc gccgcggccc
     4981 tcggcgtgga cgcggacgcc tcgctgagcg ccgtcctgcc ccggctctcc gcatggcgcc
     5041 gccaccgccg cgagcagtcc gtggtcgacg gctggcgcta ccgcgtcacc tggaagccgc
     5101 tcggcgcgct tccacggtcc ggcgcgtccg gcacctggct gctggtcgcc gccgcggaga
     5161 gcgagtggac cggctccgtc cgcaccgcgc tcgtcgaccg cggcctggac ctggtgacac
     5221 tcgtcgccgg gcccggcacc gaccgcgggg cgctggcccg cgaactggcc ggcgccggcc
     5281 cggtgaccgg cgtgctctcc ctgctcgccg aggacgacag cgcctcggga accgaccccg
     5341 cactgaccaa cgggctcgcc gcgaccctcc ggctggtgca ggcgctcggc gacgcgggca
     5401 tcgacgcgcc gctgtggtgc gccacccgcg gcgcggtggc caccggccgc tccgaccgcg
     5461 tggaccgccc gctgcagtcg caggtgtggg gcttcggccg ggccgcggcg ctcgaacacc
     5521 cgcagcgctg gggcggcctg ctcgacctgc ccgacgtgct cgacgcccgg accgccgccc
     5581 gcgtggtcgc cgtcctcggc cagacggccg aggaccaggt cgccgtccgc gcctcgggcg
     5641 ccttcggacg ccgcctcgtg cgggccgccc gcgccaccgg ccccgcccgg ggctggtcgc
     5701 cgcgcggcac ggtgctgatc acgggtggta cgggtgcgct gggcgggcac gtggcccgct
     5761 ggctcgccgg tgcgggagcg gagcacctgg tgctcgccgg ccgccgaggc ctggacgccc
     5821 ccggtgccgc cgctctgcgg gccgagctgg aggagacggg cgtcgacgtg accgtcgccg
     5881 cctgcgacgt ggccgaccgc gacgcggtgg cggccctgct cgccgtgcac cccgtcgacg
     5941 ccgtggtgca cgccgccggc gccagcgacc acgccatgat cgaggacagc gacgaggccg
     6001 ggttcgccgg cacggtcgcg gccaagacag cgggcgccac acacctgcac gaactgctgg
     6061 ccgaccggga tctcgacgcc ttcgtcatgt tctcctcggg tgccggcatc tggggcggtg
     6121 ccggccagag cgcctacgcg gcggccaaca cctacctgga cgctctcgcc gagtaccggc
     6181 gggcccgcgg gcgcgccgcg ctcgccgtct cctggggcgg ctgggccgag ggcggcatgg
     6241 ccggcgtcgg cgacggggag gagatgctgc gccgccgtgg cctgccgccg atgcgccccg
     6301 ccctggcgat ctccgcgctg cagcaggcgg tggcggacga cgagaccctc ctcaccgtcg
     6361 ccgacttcga ctggcagcgg ttcatcggcc cgttcaccgt cacccgcccc agcgccctgt
     6421 tctccgacat gcccgaggcc cgcggcgccc tggaggcccc ggcctccggc ggcgccgagg
     6481 ccggcaacgc gctcgcggcg aagctggccg tgctgtccgc gggcgagcag gaccgcacac
     6541 tggtggacct cgtccgcgcg cacgcggccg tcgtgctcgg ccacgacggc gccgcggcgg
     6601 tggagcccgg ccgggccttc aacgacctcg gcttcgactc gctgacagcc gtcgaactgc
     6661 gcaaccggct cacggccgac accggtctga agctgcccac cacgctggtc ttcgaccacc
     6721 ccgacgccac cgccctggcc cgcttcctgc gcaccgagct cctcggtgcg caggaagccg
     6781 cgccccagga gctgcacccg gccgccaccg gcggcgacga gcccatcgcg atcgtggcca
     6841 tgagctgccg cctgcccggc ggcgtgggct cgcccgagga cctgtggcag ctcgtgacgt
     6901 ccggcggcga cgcgatcacc gccttccccg aggaccgcgg ctgggacgtc gagggcctct
     6961 acgaccccga ccccggcgcg ccgggcaaga cgtacacgct cgccggcggc ttcctgcacg
     7021 aggcgggtga cttcgacgcc gcggtgttcg gggtctcgcc gcgcgaggcg atcgcgatgg
     7081 acccgcagca gcggctgctg ctggagatca cgtgggaggc gttcgagcgg gccggcatcg
     7141 ccccggcctc cctcaagggc agccgcacgg gcgtgttcgt cggcatgtcc taccagggct
     7201 acggcgccgg cctcccggag gtccccgagg gagtcgaggg ccacctgctc accggcagcg
     7261 ccgcgagcgt cgtctccggg cgcgtcgcct actcgttcgg cctggagggc ccggcggtca
     7321 ccgtcgacac ggcgtgctcg tcgtcgctgg tggccctgca cctggccatg cagtcgctgc
     7381 ggagcggcga gagcacgatg gccgtcgcgg gcggtgtgaa cgtcatggcc ctgccggccg
     7441 cgttcgtcga gttcagccgg cagcgcggac tctccgtcga cggccgctgc aaggcgttcg
     7501 gggccggcgc ggacggcacc ggctgggcgg agggcgcggg cgtggtgctc gtggagcgcc
     7561 tcagcgacgc cgtgcgcaac ggccaccagg tgctggcggt cgtgcggggc agcgcggtga
     7621 accaggacgg cgcgtccaac ggcctgaccg cgcccaacgg tcccgcgcag cagcgggtca
     7681 tccggcaggc cctggcctcc gccgggctcg gtgaggccga cgtcgacgcc gtcgaggcgc
     7741 acggcacggg cacccggctg ggtgacccga tcgaggcaca ggcgctgctc gccacctacg
     7801 ggcagcagcg gccggagggc cggccgctgt ggctgggttc gctgaagtcc aacatcggcc
     7861 acgcgcaggc cgcctccggg gtggccggtg tgatcaagat ggtgatggcg ctgcggcacg
     7921 gtgtgctgcc gcggacgctg cacgccgagg agccgacgcc gcacgtggac tggtcggcgg
     7981 gtgacgtgcg gctgctgacg gaggccgtcg actggccgga gacggaccgc ccgcgccggg
     8041 ccgccgtctc ctccttcggc gtcagcggca ccaacgccca caccatcatc gagcaggccc
     8101 cggtggccga accggccccc gtcgcaccgg tcgaggtcgc ctcggcgacc gtgccgtggg
     8161 tgctgtccgg caagtccgag gccgcgctgc gcgcccaggc cgagcgcctg ctggtccgta
     8221 ccgccgacgg caccgggccc tccctcaccg acatcggctt ctccctggcc accacccgaa
     8281 ccgccctgga gcaccgcgcc gccgtggtcg gcgacgacca cggccggctc accgagggcc
     8341 tgcgggctct ggccgagggg cggcccgcgc ccggcgccta cctcgggcgc gccgacaccg
     8401 gccaggtcgg gttcctcttc tccggtcagg gctcccagca cgccggcatg ggacgggagc
     8461 tgtacgaggc gttcccggtc ttcgcggagg cgtacgacga ggtctgcgcc cacctcgacc
     8521 ggcacctgga ccagccgctg cgggccgtgg ccttcggcga ggacggccac gaggacggcg
     8581 agcacctgaa ccgcaccgcc ttcacccagc ccgcgctgtt cgcgcacgag gtcgccgtcc
     8641 accggctgct ggagtcctgg ggtgtgcggc ccgactacct ggccgggcac tccgtgggcg
     8701 agatcgcggc cgcgcacgtg gccggggcac tcacgctgga ggacgccgcg acgctggtct
     8761 ccgcgcgggc ccggctgatg caggcgctgc ccgagggcgg ggcgatgatc gccgtcgagg
     8821 ccaccgagga cgaggtcctg ccgcacctca ccgagggcgc cggcatcgcg gccgtcaacg
     8881 gtccgcggtc cgtcgtgctc tcgggggccg aggacgccgt ggcggccgtc gccgcacact
     8941 tcgcccagga gggccgcagg acgtcccggc tcgcggtgag ccacgcgttc cactcgccgc
     9001 tgatggaccc tgtgctggag gagttcgccc gcgtcgccgc cggtctgcgc ttcgagcccc
     9061 cccggacacc ggtcgtctcc aacctcaccg gccggctcat cgactcgtac accgcggact
     9121 actgggtccg gcacgtccgc gaggccgtgc gcttcgccga cggcatcgcg acgctcggcg
     9181 agctgggtgt gaccacgttc gtggagaccg gacccggcac ggtgctcagc ggcatggccc
     9241 ggacctgcct ggacgacgcg gtcaccgtcc ccaccacccg gtccggcagc cccgggcccg
     9301 aaacaatcac cgacgccctc gcgcggctcc acgtgcacgg cgtccccgtc gactggcacg
     9361 cgtacttcgc gggccgcggc gcccgccgca ccgacctgcc cacctacgcc ttccagcacc
     9421 agcggtactg gctcacgacc accgcgccca cggccgccgc gacgggggcc gacgccgtgg
     9481 aggccgggtt ctgggagacg gtggagcgcg aggacgccca gtccctcgcc gccaccctcg
     9541 acctgcccgc cggacagctg gacgcggtgc tgccccggct gtccgcgtgg cgccggcggc
     9601 agcgccggga gtccgccgcg gaaggctggt cctaccgcac ggcctggaag ccgctcggcg
     9661 gcctgcggac gggccggctc acgggcgact ggctgttgct gtccaccgag gatcccggcg
     9721 accccgaggc caccgggtgg accgccgccg tcgccgacgg gctcaccgcc ctcggtgccc
     9781 ggctggtccg cgtggcggtc gctcccgacg ccgaccgcgg cgcactgcgg cggcagctca
     9841 ccgacgccgc cgggcggacc ccgctcgagg gcgtgctctc cctgctcggc accgacgagc
     9901 ggccgtactc cgggcacccg gccctgcgcg tcggcaccgc gctgaccctg gcgctcgtgc
     9961 aggccctggg cgacgcgggg atcgacgccc cgctgtgggc actgaccagg tccgccatgt
    10021 ccaccggccg tgcggacacg gttccgagcg ccgcccagaa cgccgtctgg ggcctgggac
    10081 gggtcgccgc gctggagcac gcggaccgct ggggcggcct gatcgacctg cccggcacga
    10141 tcgacggccg catcgtcggc cgcctcgccg cggtcctcgg ccagcgggac gaggaccagg
    10201 tcgcgatccg ccaccgcggc gtgttcggcc gccgactggc ccatgtcacc gcccgtcgcg
    10261 acaccccggc cgggcagcgc tggtcgccgc gcggcacggt gctgatcacg ggtggtacgg
    10321 gtgcgctggg cgggcacgtg gcccgctggc tcgccgaagc cggcgcggag cacctggtgc
    10381 tcaccagccg ccgcggcccc gacgcacccg gcgcggcgga cctcgtcgcg gagctggaga
    10441 cggcaggggc ccgggtcacc gtcgccgcgt gcgacgcggc cgaccgcgag gcgctcgccg
    10501 ggctgctcgc cgagcacccg gtggacgccg tcgtccacgc cgcgggcgtg gaccacctgg
    10561 acccgttgga cgccatgaca ccgggcgcct tcgccgacgc gctgtccgcc aaggcggccg
    10621 gagccctgca cctcgccgag ctgctccacg accgggagct ggacgccttc gtcctcttct
    10681 cctccatcgc cggcgtgtgg ggcagcggcc accaggccgc ctacgcggcg gccaacgccc
    10741 tgctcgacgg ggtcgccgaa cgccgccggg cccagggcct gccggcgacc gccgtggcct
    10801 ggggcccgtg ggccgacggc ggcatggccg agggcgacgg cgcggacgag cggctgcgcc
    10861 gccgcggcct gatcccgatg ccggccgcgc tcgccctggt cgggctgcgg caggcgctgg
    10921 actcgggcga ggcgaccacc acactggccg acgtcgactg gggacggttc ctgcccccgt
    10981 tcaccatggg ccgccccagc gccctcctcg gcgacctccc ggaggccgag cagatccgca
    11041 ccgcgggcgg ctcctccggc gggccgggaa cggccgccga ctcgccgctg gccggccgca
    11101 tcaccgcgct gccggagaac gaacagcaca cgctgctggt cgacctcgtc cgcacgcacg
    11161 cggcagccgt cctcgggcac agcggcatcg gcgaggtcga ggccgaccgg gccttcaagg
    11221 agctgggctt cgactcgctg accgccgtcg aactgcgcaa ccggctcaac gacgagaccg
    11281 gtctcaccct gtccgccacg ctggtcttcg accaccccaa cgcccaggca ctcgcccggc
    11341 agctgcgcac cgaactcacc ggccgcaccg gagtgccggc accggaggcc gccgccggac
    11401 ccgccgagga cgacgagccc atagccatcg tcggtatggc gtgccgttat ccgggcgggg
    11461 tgcgttcgcc cgaggatctg tg
//
