LOCUS       NZ_BMVW01000017        10411 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces poonensis strain JCM 4815 sequence17, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVW01000017
VERSION     NZ_BMVW01000017.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces poonensis
  ORGANISM  Streptomyces poonensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 212793)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 137X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650915.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:27:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,577
            CDSs (total)                      :: 7,501
            Genes (coding)                    :: 7,228
            CDSs (with protein)               :: 7,228
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 273
            CDSs (without protein)            :: 273
            Pseudo Genes (ambiguous residues) :: 0 of 273
            Pseudo Genes (frameshifted)       :: 96 of 273
            Pseudo Genes (incomplete)         :: 224 of 273
            Pseudo Genes (internal stop)      :: 19 of 273
            Pseudo Genes (multiple problems)  :: 60 of 273
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:46:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 108136
            Orig. end                         :: 118547
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVW01000017.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10411
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10411
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10411
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5411](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5411)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     assembly_gap    416..498
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <499..2720
                     /locus_tag="IE242_RS31010"
                     /old_locus_tag="GCM10010365_63540"
     CDS             <503..2720
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039575.1"
                     /locus_tag="IE242_RS31010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010365_63540"
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="WP_189865215.1"
                     /transl_table=11
                     /translation="AGLLTGEGTRYPVYIDPSVSGSRYAWAIAYKKYPDSSFYNGNGWR
                     NSDGSYGTSTARAGYENMTNGLARSYFRMRTANLWNTDKVVSRSTFRIKNTWSWSCTAK
                     PVELWRTDPIGSSTTWNDRPTRREQMDTVTDAKGWSSSCPAGNLAFDVTKGAKDAAANE
                     WDTITLELAAASESDVYGWKKFDAKTATLSTTYNTRPNVPSGLDTYPVSTNNEYGCGDR
                     APYQYIGNTDFYLRAKVSDPDGGTVKAVFRLWPTGHGDDGVILDESVSVTSGTIAKVKV
                     PKALLAEHAATASGNFSWKVKADDGTLASDWNPPLSASGCRFVFDADRASTPPTVTSSQ
                     FPDGSDGWPASTGKARTEGTFTISSGGVTDVTAYEYWTDWDSTVRTVTAGSIGGSATVK
                     ITPLAAGSHALFVRSIDQAGNKSDRGTYMFYANSPGTPDAPGDLNGDTHPDIYGVRTDG
                     SLRLYPGQGNGKFAPYTTAGSTSFDGASITHRGDWTGDGYEDLIAAQGTNGAKDLYLYP
                     NNGLGYACTSHGETTDGASAACTDKRHLLGVFDPANNHWSGAQQLLAIGDVDGPLDVDD
                     DGTPDIPGHPDLLVKEGDLLWLYFGSPDYYLDSSREPVLIGDGSWSDYDLAAPGDVDGD
                     GRVDLVARNRTAGDLRLYPGTGPNGEGLGAGSTHLTIQATGWKSTIHPLLASGGDADHD
                     GQPDLWASSPETGAELKFYPGLTASGLKAPTVVGNTGWLNFRTLS"
     misc_feature    530..532
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1778..1780
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1886..1888
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1991..1993
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2930..3979
                     /locus_tag="IE242_RS31015"
                     /old_locus_tag="GCM10010365_63550"
     CDS             2930..3979
                     /NRPS_PKS="Domain: Aminotran_5 (16-343). E-value: 6.6e-23.
                     Score: 73.1. Matches aSDomain:
                     nrpspksdomains_IE242_RS31015_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477739.1"
                     /locus_tag="IE242_RS31015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010365_63550"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_189865412.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.9e-19, bitscore:
                     61.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFGPKTTYLNTASTGLLPARTVQAMRVAVESAAVG
                     RPTDMFADVEASRSAFARIVGVSERRVAAGASVAVYTGVIAASLPAGAEVLTADADFAS
                     TVNPFHVRGDLKVRTVPLERIAESVRPGTALVAVSAAQSADGRIADLAAIREAARTHGA
                     RTYIDASQAAGWLPLDAGAYDFVSSVAFKWLVCPRGAAFLVVPEDFGGLTPVFASWVAG
                     EHPWDSCYGPVQELAHSARRFDESPALFSYAGARRSLEVVEALGVENIRRHDLALAERF
                     VAGLGALGHEPVPAPGSPVVSVPGLGHRQPELSRAGVEVSDRAGHLRAAFHLYNTPADV
                     DRLLDVLSG"
     aSDomain        2978..3958
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS31015_Aminotran_5.1"
                     /evalue="6.60E-23"
                     /label="IE242_RS31015_Aminotran_5.1"
                     /locus_tag="IE242_RS31015"
                     /protein_end="343"
                     /protein_start="16"
                     /score="73.1"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARTVQAMRVAVESAAVGRPTDMFADVEASRSAF
                     ARIVGVSERRVAAGASVAVYTGVIAASLPAGAEVLTADADFASTVNPFHVRGDLKVRTV
                     PLERIAESVRPGTALVAVSAAQSADGRIADLAAIREAARTHGARTYIDASQAAGWLPLD
                     AGAYDFVSSVAFKWLVCPRGAAFLVVPEDFGGLTPVFASWVAGEHPWDSCYGPVQELAH
                     SARRFDESPALFSYAGARRSLEVVEALGVENIRRHDLALAERFVAGLGALGHEPVPAPG
                     SPVVSVPGLGHRQPELSRAGVEVSDRAGHLRAAFHLYNTPADVDRL"
     gene            complement(4066..4995)
                     /gene="thpD"
                     /locus_tag="IE242_RS31020"
                     /old_locus_tag="GCM10010365_63560"
     CDS             complement(4066..4995)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="IE242_RS31020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010365_63560"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_189865217.1"
                     /sec_met_domain="PhyH (E-value: 1.2e-40, bitscore: 133.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTHTTTTATDTAPGVTDLYPTRGATEVATPRQDPVVWGAPDA
                     PGPIEPAGLHAFERDGFLAVDQLITEDEVAVYRQELDRLVTDPAIRADERSIVEPRSQE
                     IRSVFEVHRISEVFARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDF
                     ETWHAEDGLPNMRTVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQM
                     QDAGTPSDEALTAMASDHGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNS
                     VENTAVEPFAAPVRRPDFIGARDFTPVR"
     gene            complement(5001..5411)
                     /locus_tag="IE242_RS31025"
                     /old_locus_tag="GCM10010365_63570"
     CDS             complement(5001..5411)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577186.1"
                     /locus_tag="IE242_RS31025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010365_63570"
                     /product="ectoine synthase"
                     /protein_id="WP_189865219.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.8e-57, bitscore:
                     184.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEIEDTDRHVRAASGTWESKRILLAKDKVGFSLHETVLY
                     AGTETSMWYANHIEAVVCVEGEAELTDRDTGQTYTITPGTMYCLNEHDRHTLRVKKDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEPEEV"
     gene            complement(5485..6756)
                     /gene="ectB"
                     /locus_tag="IE242_RS31030"
                     /old_locus_tag="GCM10010365_63580"
     CDS             complement(5485..6756)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.2e-72.
                     Score: 236.7. Matches aSDomain:
                     nrpspksdomains_IE242_RS31030_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="IE242_RS31030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010365_63580"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_189865221.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.4e-79, bitscore:
                     259.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAHGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALVDYLLRDGVTHGLDMSTTAKRSFLESFQNYVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGRVPDFLWFERILEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALRELCDRQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMS
                     LCLFRPELDVWEPGEHNGTFRGNNPAFVTATAALEAYWADGSAMEKQTRARGEQVEQHL
                     ISITEENLADIKEYRGRGLVWGLEFHDKGRASRVAHRAFELGLLIETSGPEGEVVKLLP
                     ALTVTPDELDEGMTTLARAVRETV"
     aSDomain        complement(5659..6672)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS31030_Aminotran_3.1"
                     /evalue="1.20E-72"
                     /label="IE242_RS31030_Aminotran_3.1"
                     /locus_tag="IE242_RS31030"
                     /protein_end="366"
                     /protein_start="28"
                     /score="236.7"
                     /tool="antismash"
                     /translation="FDRAHGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALVDYLL
                     RDGVTHGLDMSTTAKRSFLESFQNYVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGRVPDFLWFER
                     ILEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALRELCDRQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMSLCLFRPELDVWEPGEHNGTFRGNNPAFV
                     TATAALEAYWADGSAMEKQTRARGEQVEQHLISITEENLADIKEYRGRGLVWGLEFH"
     gene            complement(6819..7367)
                     /gene="ectA"
                     /locus_tag="IE242_RS31035"
                     /old_locus_tag="GCM10010365_63590"
     CDS             complement(6819..7367)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987798.1"
                     /locus_tag="IE242_RS31035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010365_63590"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_189865223.1"
                     /transl_table=11
                     /translation="MTAAQADLPELPAEFLEMPEGLRIDRPDVADGAALWRLARDSKTL
                     DLNSSYSYLLWCRDFAGTSVVARDADGRPVGFVTGYLRPEAPQTLLVWQVTVDPAHRGR
                     GLAGALLDGLTAQVARQHPLTTLETTITPGNTASERLFASYATRHGARVEREVLFAADR
                     FPDGPHEPEVLHRIGPLSR"
     misc_feature    complement(7523..7538)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            complement(7964..9064)
                     /locus_tag="IE242_RS31040"
                     /old_locus_tag="GCM10010365_63600"
     CDS             complement(7964..9064)
                     /NRPS_PKS="Domain: Aminotran_5 (15-303). E-value: 2.2e-29.
                     Score: 94.5. Matches aSDomain:
                     nrpspksdomains_IE242_RS31040_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="IE242_RS31040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010365_63600"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_189865225.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.3e-24, bitscore:
                     78.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLSAARFASIEDRVARLLGTRQDVVITQGEALLPLE
                     GAIRGAAGPGTTALNVITGPYGQTFGDWLRDCGATVIDVSVPFHTAVTAERIREALAAH
                     PSVDFVSLVHAEAATGNTNPVAEIGAVVREHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAISVSERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAP
                     AQLEMLALEACLERIEAEGLDAVMARHAAAARATRAGVLALGGGLEPYVHEATEAAPVA
                     TTLRTPDGVVASELVARALESDPALPLAAGGGALAKEMIRVNHYGPDATPGAVQSSLAA
                     LGAALAERGLDVDLLGARRAAERAWG"
     aSDomain        complement(8156..9019)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE242_RS31040_Aminotran_5.1"
                     /evalue="2.20E-29"
                     /label="IE242_RS31040_Aminotran_5.1"
                     /locus_tag="IE242_RS31040"
                     /protein_end="303"
                     /protein_start="15"
                     /score="94.5"
                     /tool="antismash"
                     /translation="FASIEDRVARLLGTRQDVVITQGEALLPLEGAIRGAAGPGTTALN
                     VITGPYGQTFGDWLRDCGATVIDVSVPFHTAVTAERIREALAAHPSVDFVSLVHAEAAT
                     GNTNPVAEIGAVVREHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     ISVSERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAPAQLEMLALEACLERI
                     EAEGLDAVMARHAAAARATRAGVLALGGGLEPYVHEATEAAPVATTLRTPDGVVASELV
                     ARALESD"
     gene            9233..10324
                     /locus_tag="IE242_RS31045"
                     /old_locus_tag="GCM10010365_63610"
     CDS             9233..10324
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989359.1"
                     /locus_tag="IE242_RS31045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010365_63610"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_189865234.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.3e-18, bitscore:
                     60.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDRAVLRVKGRVLVGPEDVRDELWVVGGRISYERPAGARDVRTV
                     EGWVLPGLVDAHCHVGLDAHGPVPDDVSEKQALTEREAGALLLRDAGSPSDTRWIDDRD
                     DLPKIVRAGRHIARTRRYIRNYAHEIEPDDLVAYVAREARRGDGWVKLVGDWIDRDLGD
                     LAACWPRDAARAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEDTVPLF
                     AERGVAIVPTLVNIATFPRLADGGEARFPRWSAHMRRLHERRHDTVRAAYDAGVPVYVG
                     TDAGGGLAHGLVAGEVAELVTAGIPPVEALSATTWAARRWLGRPGLDEGAPADLVVYEA
                     DPRADVRVLGAPRRVVLNGKVVG"
ORIGIN
        1 cgggggcggc gacggtccgc tggtcaccgt ggtgaaggac ggccgcagca tggcctggtc
       61 ctggccggac gcgctgcccg agcccgccgt ggacggagac acggccacct acccggaggt
      121 cctgcccggg gtcgacttga agctgaaggc cggatcggcc ggcttcagcc agctcctggt
      181 cgtcaagaac gccgaggccg ccacccaccc ggagctccag agcatcgccc tcaaactgca
      241 ggccgacggc ctggacatca cagcggacga gcacggcaac ctgcaggcca ccaaccccgc
      301 cggccaagaa gtgttcaccg ccccgactcc gctgatgtgg gacagctcta ccgctccggc
      361 cgcccgcata gccagcgccc ccaccagccg cacagcggac gcgccccccg caccgnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnac gcgggcctgc tcacgggcga gggcacccgt taccccgtct
      541 acatcgaccc gtcggtgtct ggttcccgct acgcctgggc catcgcctac aagaagtatc
      601 ccgacagctc gttctacaac ggcaacggct ggcggaactc tgacggcagc tacggcacct
      661 ccaccgcccg cgccggctac gagaacatga ccaacgggct ggcgcgctcc tactttcgga
      721 tgcgcaccgc caacctgtgg aacaccgaca aggtggtctc ccgctcgacc ttccggatca
      781 agaacacctg gtcctggtcg tgcaccgcca agcccgtcga gctatggcgc accgacccca
      841 tcggctcgtc caccacatgg aacgaccggc ccacccgccg cgagcagatg gacaccgtca
      901 ccgacgccaa gggctggagc tcctcctgtc cggccggtaa ccttgcgttc gacgtcacca
      961 agggcgccaa ggacgcggcc gcgaacgagt gggacaccat caccctcgaa ctcgccgccg
     1021 ccagcgagtc cgacgtctat ggctggaaga agttcgacgc caagactgcc actctgtcca
     1081 ccacctacaa cacccggccg aacgttccca gcggcctgga cacctacccg gtctccacca
     1141 acaacgagta cggctgcggt gaccgggccc cctaccagta catcggcaac accgacttct
     1201 acctgcgggc caaggtgtcc gatcccgatg gcgggaccgt caaggccgtc ttccgcctgt
     1261 ggcccaccgg ccatggcgat gacggagtca tcctggacga gtccgtctcg gtcacctccg
     1321 gcacgattgc caaggtcaag gttcccaagg cgctgctcgc cgagcacgcc gccaccgcct
     1381 ccggcaactt ctcctggaag gtcaaggccg acgacggcac gctcgccagc gactggaatc
     1441 cccctctgag cgcctccggc tgccgcttcg tcttcgacgc cgaccgcgcc agcaccccgc
     1501 ccaccgtcac ctccagccag ttccccgacg gcagcgatgg ctggcccgcc agcaccggca
     1561 aggcccgcac tgagggcacc ttcaccatct ccagcggcgg cgtgaccgac gtcaccgcgt
     1621 acgagtactg gaccgactgg gactccaccg tgcgcaccgt caccgccggt tctataggcg
     1681 gctcggccac cgtcaagatc accccgctcg ccgcgggcag ccacgccctg ttcgtccgca
     1741 gcatcgacca ggccggcaac aagtctgacc gcggcactta catgttctac gcgaacagcc
     1801 ctggcacccc ggacgccccg ggcgacctca acggcgatac ccaccccgac atctacggcg
     1861 tacgcaccga cggttcgctg cgtctttacc ccggacaggg caacgggaag ttcgccccct
     1921 acacgaccgc aggctccacc agcttcgacg gtgcctccat cacccaccgg ggcgactgga
     1981 ccggcgacgg ttacgaggac ctgatcgccg ctcagggaac gaacggcgcg aaggacctct
     2041 acctctaccc caacaacggt ctgggctacg cctgcaccag ccatggcgag accacggacg
     2101 gggcttccgc ggcgtgcacc gataagcgcc acttgctcgg cgtcttcgac ccggccaaca
     2161 accactggtc cggcgcccag cagcttctcg ccatcggcga cgtcgacggc cccctcgacg
     2221 tcgacgacga cggcacccca gacatccccg gccaccctga cctcctggtc aaggagggcg
     2281 atctgctctg gctctacttc ggctcaccgg actactacct cgacagcagc cgtgaaccgg
     2341 ttctcatcgg tgacggcagc tggtccgact acgacctcgc cgctcctggc gacgtcgacg
     2401 gagacggccg cgtcgacctg gtcgcacgca accgtactgc cggcgacctt cgtctgtatc
     2461 cgggcactgg gcccaacggt gaaggccttg gagctggctc cacgcacctg accatccagg
     2521 ccaccggttg gaagagcacc atccatcccc tgctcgcctc cggcggcgac gccgaccatg
     2581 acggccagcc agatctgtgg gccagcagcc ccgaaaccgg cgccgaactg aagttctacc
     2641 ccggcctcac cgccagtggc ctgaaagccc ccactgtcgt gggcaacacc ggctggctca
     2701 acttccgaac ccttagctag cctgccagcc actccgtcaa caccacgact gccgtcccgg
     2761 ccaggacact cggccggggc ggcaggagcg gagcgggatg tcggaccggt gtcggagttg
     2821 tggtttcccg cggcaccgtt tccgtacacg ggtgttccga tgggcatgtc cgcgatgatt
     2881 ctcgatggac gacgcgttca gtagactcca gagtggtccc atggagccca tggagacctt
     2941 cgagagcctc gtccgcgccg agttcggccc gaagaccacc tacctcaaca ccgccagcac
     3001 cgggctgctc ccggcccgca cggtgcaggc catgagggtg gccgtggagt cggccgcggt
     3061 cggccggccc accgacatgt tcgccgacgt ggaggcgtcc cgctccgcct tcgcccggat
     3121 cgtgggcgtc tccgagcgcc gcgtcgcggc cggcgcctcg gtcgccgtct acaccggggt
     3181 gatcgccgcc tccctgcccg cgggcgccga agtcctcacc gccgacgcgg acttcgcctc
     3241 caccgtgaac cccttccacg tgcgcggcga cctgaaggtg cgcaccgtgc cgctggagcg
     3301 gatcgccgag tcggtgcgcc ccggcacggc gctcgtcgcg gtcagcgccg cccagtccgc
     3361 cgacggccgg atcgccgacc tggccgcgat ccgcgaggcg gcgcgcacgc acggggcgcg
     3421 cacgtacatc gacgcgtccc aggccgccgg ctggctgccc ctggacgccg gcgcgtacga
     3481 cttcgtctcg tccgtcgcct tcaagtggct cgtctgccca cgcggcgcgg ccttcctcgt
     3541 cgtcccggag gacttcggcg gactcacgcc cgtcttcgcg agctgggtcg cgggggagca
     3601 cccgtgggac agctgctacg gacccgtcca ggagctcgcc cactccgcgc gcaggttcga
     3661 cgagagcccc gccctcttct cgtacgcggg tgcgcgccgc tccctggagg tcgtcgaggc
     3721 cctgggcgtg gagaacatcc ggcggcacga cctggcgctc gccgagcgct tcgtcgcggg
     3781 cctcggcgcg ctcggccacg aacccgtccc ggcacccggc tcgcccgtcg tctcggtgcc
     3841 cggactcggc caccggcagc ccgaactgag ccgggccgga gtcgaggtct ccgaccgcgc
     3901 gggccacctg cgggcggcct tccacctgta caacactccc gcggacgtcg accggctcct
     3961 cgacgtcctg tccggctgat ccggctgacg cccgcgggcc gggcacccct ggcagggtgc
     4021 ccggcccgtc gatgcggccg gtacggcaag tacggaagac gtacgtcagc gcaccggggt
     4081 gaagtcccgc gccccgatga agtccggccg ccgcaccggc gccgcgaacg gctccaccgc
     4141 cgtgttctcc acgctgttga acacgatgaa gacattgctg cgcgggaacg gcgtgatgtt
     4201 gtcgccggag ccgtgcatgc agttgcagtc gaaccaggtc gccgaacccg ccttgcccgt
     4261 gaacagcttg atgccgtggt cggaggccat ggcggtgagc gcctcgtcgg acggcgtgcc
     4321 cgcgtcctgc atctggagcg acttcttgta gttgtccttc ggtgtctccc ccgcacagcc
     4381 gaggaacgtc ttgtgcgacc ccggcatgat catgagtccg ccgttggtgt cgaagttctc
     4441 ggtcagcgcg atcgacacgg acaccgtgcg catgttcggc agaccgtcct cggcgtgcca
     4501 ggtctcgaag tccgagtgcc agtagaagcc ggaggcgccg aagcccggct tgacgttgat
     4561 ccgcgactgg tggacgtaca cgtccgagcc gaggatctgc cgcgcccgcc ccacgacgcg
     4621 ctcgtcgcgc accagccggg cgaagacctc gctgatcctg tgcacctcga agaccgaccg
     4681 gatctcctgg gaccgcggct cgacgatcga gcgctcgtcg gcgcggatcg ccgggtcggt
     4741 cacgagccgg tccagctcct gccggtagac ggccacctcg tcctcggtaa tgagctggtc
     4801 gacggcgagg aaaccgtcac gctcgaacgc gtgcaggccg gccggctcga tcgggccggg
     4861 cgcgtcgggg gcgccccaga cgaccgggtc ctggcggggg gtggcgacct cggtggcgcc
     4921 gcgggtgggg tagaggtcgg tgacgcccgg ggcggtgtcc gtcgcggtgg tggtcgtgtg
     4981 cgtggtcgtg gtcatgggga tcacacctcc tcgggctcgg gctcggtgag cagcgggtag
     5041 acgccgttct cgtcgtggtc ctcgcggccg gtcacgggcg ggttgaagac gcagatgcag
     5101 cggaagtcct tcttgacccg cagcgtgtgc ctgtcgtgct cgttcaggca gtacatggtg
     5161 ccgggcgtga tggtgtacgt ctgcccggtg tcgcggtcgg tcagttcggc ctcgccctcc
     5221 acgcagacga cggcctcgat gtggttggcg taccacatcg acgtctccgt gcccgcgtac
     5281 aggacggtct cgtgcagcga gaagccgacc ttgtccttgg cgagcaggat gcgcttgctc
     5341 tcccaggtgc cggacgcggc ccgcacatgc cggtcggtgt cctcgatctc cttgaacgaa
     5401 cggacgatca cggtgtttcc tccgcctcct tgaggtgtcg cgggtgtcgc gggtgttttg
     5461 gatatctcgg gtgtcgcggg tgtttcagac ggtctcgcgg accgcgcggg cgagggtcgt
     5521 catgccctcg tccagctcgt cgggcgtgac ggtgagggcg gggagcagct tgacgacctc
     5581 gccctcgggg cccgaagtct cgatcagcag cccgagttcg aaggcacgat gcgcgacacg
     5641 cgacgcccgc cccttgtcgt ggaactccag gccccacacc aggccgcggc cccggtactc
     5701 cttgatgtcg gcgaggttct cctcggtgat ggagatcagg tgctgctcga cctgctcgcc
     5761 gcgggcgcgg gtctgcttct ccatcgccga cccgtcggcc cagtacgcct ccagcgcggc
     5821 ggtcgccgtg acgaaggcgg ggttgttgcc gcggaaggtg ccgttgtgct cgcccggctc
     5881 ccacacgtcc agctcgggcc tgaacaggca cagcgacatg ggcaggccgt agccgctgat
     5941 cgacttggag acggtgacga tgtccggcac gatcccggcc tcctcgaagg agaagaaggc
     6001 accggtacgg ccgcagccca tctggatgtc gtcgacgatc agcagcatgt cctgccggtc
     6061 gcacagctcc cgcagggcgc gcagccactc gggccgggcg acgttgatgc cgccctcgcc
     6121 ctgcacggtc tcgacgatca cggcggcggg cttgttgagg ccggagccct ggtcctccag
     6181 gatgcgctcg aaccagagga agtccgggac ccggccgtcg aagtagttgt cgaacggcat
     6241 cggcgtgccg tgcaccagcg gaatgccggc gccggcccgc ttgaaggcgt tgccggtcac
     6301 ggcgagcgag cccagcgaca tcccgtggaa ggcgttggtg aacgacacga tggcctcgcg
     6361 ccccttcacc ttccgcgcca gcttcagcgc cgactccacg gcgttggtgc cggtcgggcc
     6421 cgggaacatg accttgtacg gcaggtcgcg cggccgcagc acgtagttct ggaacgactc
     6481 caggaagctg cgtttggcgg tggtcgacat gtcgagtccg tgggtgacgc cgtcgcggag
     6541 gaggtagtcg accagggctc gtttgagtac ggggttgttg tggccgtagt tgagtgatcc
     6601 ggcaccggcg aagaagtcga ggtactcgtg gccgtcctcg tcgtacatgc ggctgccgtg
     6661 cgcacggtcg aagacggtgg gccagccgcg gcagtagctg cgcacctccg actccagggt
     6721 ttcgaagacg ctgaggtcgg gctgggtgat ggtcacggcg agatcgctcc ttggggcgtg
     6781 gtggtcggca agggggtctg gcttgtcggc cgggccgctc agcgggacag cgggccgatg
     6841 cggtgcagga cctcgggttc gtgcgggccg tccgggaacc ggtccgccgc gaacagcacc
     6901 tcgcgctcga cacgcgcccc gtggcgggtg gcgtacgagg cgaacagacg ctccgaggcg
     6961 gtgttgcccg gggtgatcgt ggtttccagg gtggtcagcg ggtgctgccg ggcgacctgg
     7021 gcggtcagcc cgtccagcag cgccccggcg agaccgcggc cccggtgcgc cgggtcgacg
     7081 gtgacctgcc acaccaggag ggtctgcggg gcctcggggc ggaggtagcc ggtgacgaag
     7141 ccgacgggcc gtccgtccgc gtcccgggcc accaccgagg tgccggcgaa gtcacggcac
     7201 cacaggagat agctgtagga ggagttcagg tcgagggtct tggagtccct ggcgagacgc
     7261 cacagggcgg cgccgtccgc cacgtcagga cggtcgatcc ggagcccctc cggcatttcc
     7321 aggaattccg cgggcagttc tggcaggtct gcttgagcgg ctgtcatgcg aattcaactt
     7381 acccagcgaa agttgaaatt gcaccgtgag tcggggttac ggatggggct tgtgtgtgtt
     7441 atcgcgcggg cgtgtgtggc cgcgcgacag ggcgatggaa ggtgccattt tgcccggaaa
     7501 ataccggcga aaaggggcgc gctgtggaat cgatcacaca gctgtaatca ccatggaatc
     7561 cacccgaatt acttcgccgg gtgttctcgg aatcttcgcg tttaggcttg aggaaagcgg
     7621 gcagaagagc acgggaagct gcgctgaatc aatccgcgtg gacaagggga gggatactgc
     7681 ggaaaggctg cgccgctgtc aatggcggca atggcggcaa tagcggcaat ggtggagtgg
     7741 aagccgggtc ccgcgccggt ggtccagccg agccgcgatc ccgtacgagg cccgccgtgg
     7801 ccgtggccgt ggccgtggct atggccgtgt gaggtgtgag tgcggcggtg tgaggtgcgg
     7861 cggcgggcat gtgcgcacgc gcagcatcgc gctgcccgct cggtcctcag tccctcgggc
     7921 caccgtcccg gcccgcaccc ccgcccgcga cggcggcgaa ccgctacccc caggcccgct
     7981 ccgccgcccg ccgcgcaccg agaaggtcca cgtccagtcc ccgctcggcc agcgccgcgc
     8041 ccagtgccgc cagactcgac tggacggcgc ccggcgtggc gtcgggaccg tagtggttga
     8101 cccggatcat ctccttcgcc agcgcgcccc cgcccgcggc cagcggcagc gcggggtccg
     8161 actccagggc ccgcgcgacc agctccgagg cgacgacacc gtccggcgtg cgcagcgtcg
     8221 tggcgaccgg cgcggcctcc gtggcctcgt gcacgtacgg ctccaggccc ccgccgaggg
     8281 cgagcacgcc cgcgcgggtc gcccgcgccg cggccgcgtg ccgggccatc accgcgtcca
     8341 gcccctcggc ctcgatccgc tccaggcacg cctccagcgc gagcatctcc agctgtgccg
     8401 gggcgtgcag cagggcacgg cggccgccgt cgatccaccg ctccttccag tccagcaggg
     8461 acaggtacga gcggcgcggc gcccgcgggt tggccgccat gcgcgcccac gcccgctcgc
     8521 tcaccgagat ggccgacacc cccgcgggac cgcccatcgc cttctgcgcc ccgatcacgc
     8581 acaggtccac gccccacgcg tccggcagca ccggctccgc cccgaccgag gcgaccgcgt
     8641 ccaggtagaa gagcgcgccg tgctcccgga ccaccgcgcc gatctccgcg accgggttgg
     8701 tgttgccggt cgccgcctcc gcgtgcacca gcgacacgaa gtcgaccgac gggtgcgcgg
     8761 cgagcgcctc ccggatccgc tccgccgtga ccgcggtgtg gaacggcacg gacacatcga
     8821 tcaccgtggc cccgcagtcg cgcagccagt cgccgaaggt ctgcccgtac ggcccggtga
     8881 tcacgttcag tgcggtggtg cccgggcccg cggccccgcg gatggcgccc tccagcggca
     8941 gcagggcctc gccctgggtg atcaccacgt cctgccgggt gccgagcagc cgcgccacgc
     9001 ggtcctcgat cgaggcgaag cgcgccgcgc tcagcggcgg cagatccagg aaggggtgcg
     9061 tcacggtggt gctctcttcg ctcgtggata ccggtggata ccgatggtgg gcggtacgcg
     9121 cagtaccggt aagaggttat ccggcggccc tcggagctcc tcctgggacg aggtccgcgc
     9181 gcggggcgac cggccggacg aggcgggcga cggcccaact aaggtgccgt gcatgagcga
     9241 tcgcgcggtg ctgcgggtga aggggcgggt gctcgtcggg cccgaggacg tccgggacga
     9301 actgtgggtc gtcggcggca ggatctcgta cgagcgtccc gccggcgccc gtgacgtccg
     9361 taccgtcgag ggctgggtcc tgcccggcct ggtcgacgcg cactgccacg tcggactgga
     9421 cgcccacggc cccgtgcccg acgacgtctc ggagaagcag gccctcaccg agcgcgaggc
     9481 gggcgcgctg ctcctgcggg acgcgggctc cccgtccgac acccgctgga tcgacgaccg
     9541 cgacgacctg ccgaagatcg tccgagcggg ccggcacatc gcccgcaccc gccgctacat
     9601 ccgcaactac gcccacgaga tcgagccgga cgacctggtc gcgtacgtag cccgggaggc
     9661 ccggcgcggc gacggctggg tgaagctggt gggcgactgg atcgaccgcg acctcggcga
     9721 cctggcggcg tgctggccac gggacgccgc ccgggcggcg atcgcggagg cccaccgcct
     9781 cggcgcccgc gtcacggccc actgcttcgc cgaggactcc ctgcgcgacc tggtggaggc
     9841 gggcatcgac tgtgtcgagc acgcgacggg cctgacggag gacaccgtcc cgctcttcgc
     9901 cgagcggggc gtggcgatcg tccccaccct cgtcaacatc gcgacgttcc cgcggctcgc
     9961 ggacggcggc gaggcgcggt tcccgcgctg gtccgcacat atgcgccgac tgcacgaacg
    10021 ccgtcacgac accgtgcggg ccgcgtacga cgccggggtc cccgtgtacg tcggcaccga
    10081 cgccggcggc gggctggcgc acggactggt ggccggggag gtcgccgaac tggtgaccgc
    10141 gggcatcccc ccggtcgagg ccctgtcggc gacgacgtgg gctgcgcggc gctggctcgg
    10201 ccgccccggg ctcgacgagg gggcgcccgc ggacctggtc gtgtacgagg cggatccgcg
    10261 ggcggacgta cgcgtgctgg gggcgccgcg gcgggtggtg ctgaacggga aggtcgtcgg
    10321 ctagaccccg tacccgggcg cccgggccgg tgtgacgcct gtgctccggg actcgaagga
    10381 gcgtgcgccc atcgttcagc catgtgcgga a
//
