LOCUS       NZ_BMVR01000051         4424 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces flavofungini strain JCM 4753 sequence51, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVR01000051
VERSION     NZ_BMVR01000051.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces flavofungini
  ORGANISM  Streptomyces flavofungini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650815.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:46:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,873
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,638
            CDSs (with protein)               :: 7,638
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 43 of 161
            Pseudo Genes (incomplete)         :: 137 of 161
            Pseudo Genes (internal stop)      :: 10 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4424
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVR01000051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS_motif       complement(<1..47)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0013"
                     /evalue="1.50E-06"
                     /label="PKSI-DH_m2"
                     /locus_tag="IE274_RS39015"
                     /protein_end="1475"
                     /protein_start="1459"
                     /score="20.8"
                     /tool="antismash"
                     /translation="WLADHAIQGAVLLPGT"
     source          1..4424
                     /culture_collection="JCM:4753"
                     /db_xref="taxon:68200"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces flavofungini"
                     /strain="JCM 4753"
                     /submitter_seqid="sequence51"
                     /type_material="type strain of Streptomyces flavofungini"
     gene            complement(<1..>4424)
                     /locus_tag="IE274_RS39015"
     region          1..4424
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4424
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4424
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:4424](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..4424)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             complement(<1..4424)
                     /NRPS_PKS="Domain: PKS_KR (195-370). E-value: 3.7e-60.
                     Score: 194.9. Matches aSDomain:
                     nrpspksdomains_IE274_RS39015_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (462-531). E-value: 3.1e-32. Score:
                     102.7. Matches aSDomain:
                     nrpspksdomains_IE274_RS39015_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (555-980). E-value:
                     1.3e-188. Score: 619.1. Matches aSDomain:
                     nrpspksdomains_IE274_RS39015_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (1081-1361). E-value: 1.3e-102.
                     Score: 335.1. Matches aSDomain:
                     nrpspksdomains_IE274_RS39015_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060784.1"
                     /locus_tag="IE274_RS39015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_190120699.1"
                     /sec_met_domain="adh_short (E-value: 1.4e-65, bitscore:
                     206.6, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 5.7e-09,
                     bitscore: 21.5, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 8.1e-18, bitscore:
                     51.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 1.6e-234, bitscore:
                     764.5, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 7.2e-101,
                     bitscore: 323.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.2e-104, bitscore:
                     335.1, seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRPVSAALSRQGWESLFHLDWVPAQTATSSAGTAGPVAEFADWA
                     AVRSELDAGESLPDNVVVRCAESADVASVRPQVLAALELVRGWLADDRCMDSRLVLVTR
                     GAVAVEPGEDVPDLAASAVWGLVRTAQAENPDRIVLVDVDGDVDGGDENTWKPVLTSGE
                     PQIVVRDGRAHVARLARLRPEPGTDPAFGPDSSVLVTGASGALGGLVARHLVDSHGVGR
                     LVLASRRGAAAEGAEELRAELTAQGAEVTYAACDVADRDALAALLAEHPVTAVVHTAGV
                     LDDGTIGSLTPDQVDTVFRPKVDAAWHLHELTRESDLTAFVLFSSVAGTLGGPGQGNYS
                     AANAFLDALAQRRRAAGLPATSLAWGLWADGMGARVAQSAATGLSAEEGVALFDAAVSG
                     TAPVVVPMRLDLRAVRELPQIPPVFSGLVRTTTRRAAGTGADPAAALRALLAPLAEDER
                     ARTLLDLVRTQAATVLGHAGPEAVAPEQAFTELGFDSLTAVDLRNRLNAATGLRLPATL
                     IFDYPTPAAIAAFVGVEIAGERVAAPATVAAVTATDEPIAIVGMSCRYPGGVNTPEDLW
                     RLLVDGAEGITPFPTERGWDVEALYDPAGERVGSSYTREGGFLHDADRFDPGFFGISPR
                     EALAMDPQQRLLLETSWEAFERAGIDPQSLRGSATGVFAGVMYHDYASQLYGVPEGVES
                     FLGTGNAGSVASGRVSYVFGMEGPAMTVDTACSSSLVALHLAAQALRSGECSLALAGGV
                     TVMATPSAFIDFSRQQGLSPDGRCRSFSTDANGTGWGEGVGMLVLERLSDARRNGHHVL
                     AVLSGSAVNQDGASNGLTAPNGPSQQRVIRQALASAGLTPSDVDAVEAHGTGTTLGDPI
                     EAQALLATYGRDRTGDPLWLGSLKSNIGHTQAAAGVGGVIKTVMALRNGLLPKTLHVGE
                     PSPHIDWSAGAVELLTEARPWPQTDRPRRAGVSSFGFSGTNAHVIIESAPEAAAPAAAE
                     SAPLGPWLVSGRTESALHDQALRLRDHLTARPDLDPADVARTLATSRAAFEHRLAVVGR
                     NSADLLAGLTDAIEGQVTSGVVRGVAYERRVGFLFSGQGSQRLGMGRELAARFPVFAEA
                     LDEVLGQFEPAVREVLFGEDADALNETGVTQPALFAVEVALFRLLESWGVRPDVLAGHS
                     IGELAAAHVSGVWSLADAAKVVSARGALMQALPSGGAMVAIQATEAEVASDLPETVGVA
                     AVNGPASVVVSGVAADVDGVAERWREAGRKATRLKVSHAFHSPLMDPMLDDFRAVLESV
                     SYAAPKLSIVSTLTGKPVSGAELGSPEYWVRHVRESVRFADAVADMGADVLVEIGPGGV
                     LSALGQESLPDAAFVPVLRGDRPEDSAVMTALAELDVRGVRVDWDAYYQGFGHAVDLPT
                     YAFQYQRFWPDTSLRPATDATGLGLGSADHPLLGAAVALADEDTVLFTGRLSVGTHPWL
                     ADHAIQGAVLLPGT"
     aSDomain        complement(342..1181)
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS39015_PKS_AT.1"
                     /evalue="1.30E-102"
                     /label="IE274_RS39015_PKS_AT.1"
                     /locus_tag="IE274_RS39015"
                     /protein_end="1361"
                     /protein_start="1081"
                     /score="335.1"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFSGQGSQRLGMGRELAARFPVFAEALDEVLGQFEPAVREVLFGE
                     DADALNETGVTQPALFAVEVALFRLLESWGVRPDVLAGHSIGELAAAHVSGVWSLADAA
                     KVVSARGALMQALPSGGAMVAIQATEAEVASDLPETVGVAAVNGPASVVVSGVAADVDG
                     VAERWREAGRKATRLKVSHAFHSPLMDPMLDDFRAVLESVSYAAPKLSIVSTLTGKPVS
                     GAELGSPEYWVRHVRESVRFADAVADMGADVLVEIGPGGVLSALGQESLPDAAFVPVL"
     aSModule        complement(342..2759)
                     /domains="nrpspksdomains_IE274_RS39015_PKS_KS.1"
                     /domains="nrpspksdomains_IE274_RS39015_PKS_AT.1"
                     /incomplete
                     /locus_tags="IE274_RS39015"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(456..491)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0012"
                     /evalue="2.00E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IE274_RS39015"
                     /protein_end="1323"
                     /protein_start="1311"
                     /score="14.7"
                     /tool="antismash"
                     /translation="YWVRHVRESVRF"
     CDS_motif       complement(714..752)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0011"
                     /evalue="6.90E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE274_RS39015"
                     /protein_end="1237"
                     /protein_start="1224"
                     /score="12.9"
                     /tool="antismash"
                     /translation="AAVNGPASVVVSG"
     CDS_motif       complement(837..959)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0010"
                     /evalue="2.50E-20"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="IE274_RS39015"
                     /protein_end="1196"
                     /protein_start="1155"
                     /score="64.7"
                     /tool="antismash"
                     /translation="GVRPDVLAGHSIGELAAAHVSGVWSLADAAKVVSARGALMQ"
     CDS_motif       complement(969..1028)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0009"
                     /evalue="1.60E-04"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE274_RS39015"
                     /protein_end="1152"
                     /protein_start="1132"
                     /score="14.8"
                     /tool="antismash"
                     /translation="ETGVTQPALFAVEVALFRLL"
     CDS_motif       complement(1134..1184)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0008"
                     /evalue="2.90E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE274_RS39015"
                     /protein_end="1097"
                     /protein_start="1080"
                     /score="16.6"
                     /tool="antismash"
                     /translation="FLFSGQGSQRLGMGREL"
     aSDomain        complement(1485..2759)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS39015_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.30E-188"
                     /label="IE274_RS39015_PKS_KS.1"
                     /locus_tag="IE274_RS39015"
                     /protein_end="980"
                     /protein_start="555"
                     /score="619.1"
                     /tool="antismash"
                     /translation="IAIVGMSCRYPGGVNTPEDLWRLLVDGAEGITPFPTERGWDVEAL
                     YDPAGERVGSSYTREGGFLHDADRFDPGFFGISPREALAMDPQQRLLLETSWEAFERAG
                     IDPQSLRGSATGVFAGVMYHDYASQLYGVPEGVESFLGTGNAGSVASGRVSYVFGMEGP
                     AMTVDTACSSSLVALHLAAQALRSGECSLALAGGVTVMATPSAFIDFSRQQGLSPDGRC
                     RSFSTDANGTGWGEGVGMLVLERLSDARRNGHHVLAVLSGSAVNQDGASNGLTAPNGPS
                     QQRVIRQALASAGLTPSDVDAVEAHGTGTTLGDPIEAQALLATYGRDRTGDPLWLGSLK
                     SNIGHTQAAAGVGGVIKTVMALRNGLLPKTLHVGEPSPHIDWSAGAVELLTEARPWPQT
                     DRPRRAGVSSFGFSGTNAHVIIESAP"
     CDS_motif       complement(1707..1751)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0007"
                     /evalue="1.70E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE274_RS39015"
                     /protein_end="906"
                     /protein_start="891"
                     /score="11.0"
                     /tool="antismash"
                     /translation="GSLKSNIGHTQAAAG"
     CDS_motif       complement(1809..1853)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0006"
                     /evalue="3.80E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE274_RS39015"
                     /protein_end="872"
                     /protein_start="857"
                     /score="12.7"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       complement(2229..2276)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0005"
                     /evalue="3.20E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE274_RS39015"
                     /protein_end="732"
                     /protein_start="716"
                     /score="22.1"
                     /tool="antismash"
                     /translation="GPAMTVDTACSSSLVA"
     aSDomain        complement(2832..3038)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS39015_ACP.1"
                     /evalue="3.10E-32"
                     /label="IE274_RS39015_ACP.1"
                     /locus_tag="IE274_RS39015"
                     /protein_end="531"
                     /protein_start="462"
                     /score="102.7"
                     /tool="antismash"
                     /translation="LDLVRTQAATVLGHAGPEAVAPEQAFTELGFDSLTAVDLRNRLNA
                     ATGLRLPATLIFDYPTPAAIAAFV"
     aSModule        complement(2832..3839)
                     /domains="nrpspksdomains_IE274_RS39015_PKS_KR.1"
                     /domains="nrpspksdomains_IE274_RS39015_ACP.1"
                     /incomplete
                     /locus_tags="IE274_RS39015"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(3315..3839)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS39015_PKS_KR.1"
                     /evalue="3.70E-60"
                     /label="IE274_RS39015_PKS_KR.1"
                     /locus_tag="IE274_RS39015"
                     /protein_end="370"
                     /protein_start="195"
                     /score="194.9"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="SVLVTGASGALGGLVARHLVDSHGVGRLVLASRRGAAAEGAEELR
                     AELTAQGAEVTYAACDVADRDALAALLAEHPVTAVVHTAGVLDDGTIGSLTPDQVDTVF
                     RPKVDAAWHLHELTRESDLTAFVLFSSVAGTLGGPGQGNYSAANAFLDALAQRRRAAGL
                     PATSLAWGLWAD"
     CDS_motif       complement(3363..3467)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0004"
                     /evalue="8.50E-21"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE274_RS39015"
                     /protein_end="354"
                     /protein_start="319"
                     /score="66.6"
                     /tool="antismash"
                     /translation="AFVLFSSVAGTLGGPGQGNYSAANAFLDALAQRRR"
     CDS_motif       complement(3495..3524)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0003"
                     /evalue="6.10E-03"
                     /label="PKSI-KR_m3"
                     /locus_tag="IE274_RS39015"
                     /protein_end="310"
                     /protein_start="300"
                     /score="10.1"
                     /tool="antismash"
                     /translation="PKVDAAWHLH"
     CDS_motif       complement(3738..3839)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0002"
                     /evalue="4.50E-07"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE274_RS39015"
                     /protein_end="229"
                     /protein_start="195"
                     /score="22.5"
                     /tool="antismash"
                     /translation="SVLVTGASGALGGLVARHLVDSHGVGRLVLASRR"
     CDS_motif       complement(4320..4349)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39015_0001"
                     /evalue="6.00E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE274_RS39015"
                     /protein_end="35"
                     /protein_start="25"
                     /score="-2.4"
                     /tool="antismash"
                     /translation="QTATSSAGTA"
ORIGIN
        1 gtgccgggca gcagcaccgc accctggatg gcgtggtcgg ccagccacgg gtgggttccc
       61 accgacagcc tgcccgtgaa caggacggtg tcctcgtccg cgagcgcgac cgcggcgccg
      121 agcagcgggt ggtcggcgga gccgagcccg agtccggtcg cgtcggtggc gggccgcagg
      181 ctcgtgtcgg gccagaaccg ctggtactgg aaggcgtacg tcggcaggtc gacggcgtgg
      241 ccgaagccct ggtagtacgc gtcccagtcg acgcgcacgc cgcgtacgtc cagttcggcg
      301 agcgcggtca tgaccgcgct gtcctccggc cggtcgccgc ggagcaccgg cacgaaggcc
      361 gcgtccggca gggactcctg acccagtgcc gagaggacgc caccggggcc gatctcgacg
      421 aggacgtccg cgcccatgtc ggccacggcg tcggcgaacc gcacggactc gcgcacgtgc
      481 cgcacccagt actccggcga tcccagctcc gccccggaca ccggcttgcc ggtcagggtg
      541 gacacgatcg agagcttggg cgccgcgtac gacacgctct ccaggaccgc gcggaagtcg
      601 tccagcatcg ggtccatcaa cggcgagtgg aacgcgtggc tgaccttcag ccgggtcgcc
      661 ttgcgacccg cctcacgcca ccgctccgcg accccgtcga catcggcggc gacaccggag
      721 acgaccaccg aggcggggcc gttgaccgcc gcgacaccga ccgtctccgg caggtccgac
      781 gccacctcgg cctccgtggc ctggatcgcc accatcgcac caccggacgg cagcgcctgc
      841 atcagcgcac cgcgcgccga caccaccttc gcggcatccg cgagcgacca cacgcccgag
      901 acgtgcgcgg ccgccagctc gccgatcgag tgtccggcca ggacatccgg ccgcacaccc
      961 cacgactcca gcaagcggaa cagcgccacc tcgaccgcga acaacgcggg ctgcgtcacc
     1021 ccggtctcgt tcagggcgtc cgcgtcctca ccgaacagca cctcacgtac cgccggttcg
     1081 aactgcccca gcacctcgtc caaggcctcg gcgaacaccg ggaagcgggc cgcgagttca
     1141 cggcccatgc cgagccgctg cgagccctgc cccgagaaca ggaagccgac ccggcgctcg
     1201 taggcgacac cgcgtacgac tccgctcgtc acctgcccct cgatggcgtc ggtgagcccg
     1261 gcgagcaggt cggccgagtt ccggccgacg acggcgagcc ggtgctcgaa cgcggcccgc
     1321 gaggtcgcga gcgtccgggc gacatcggcc gggtcgaggt cgggccgcgc ggtcaggtgg
     1381 tcgcgcagcc gcagcgcctg gtcgtgcagg gcgctttcgg tgcggccgga gaccagccac
     1441 ggaccgagcg gggccgactc ggctgccgcc ggtgcggcgg cttccggcgc ggactcgatg
     1501 atgacgtgcg cgttggtgcc gctgaagccg aaggacgaca caccggcccg ccggggccgg
     1561 tccgtctgcg gccacggccg cgcctcggtg aggagctcca cggcgcccgc ggaccagtcg
     1621 atgtgcggcg agggctcacc gacgtgcagg gtcttcggca gcaggccgtt ccgcagcgcc
     1681 atgaccgtct tgatgacgcc gccgacaccg gcggcggcct gggtgtggcc gatgttggac
     1741 ttcagcgaac ccagccacag cggatcgccg gtgcggtccc gcccgtacgt ggcgagcagg
     1801 gcctgcgcct cgatcgggtc gcccagcgtg gtgccggtgc cgtgcgcctc gaccgcgtcc
     1861 acgtcggacg gcgtgagacc ggcgctcgcg agggcctggc ggatcacccg ctgctgcgag
     1921 ggaccgttcg gcgcggtgag gccgttggac gcgccgtcct ggttgaccgc cgagccgctc
     1981 agcaccgcga gcacgtggtg cccgttgcgc cgggcgtcgg agaggcgttc caggacgagc
     2041 atgccgacgc cctcgcccca gccggtgccg ttggcgtccg tggagaacga gcggcaccgg
     2101 ccgtccggcg aaaggccctg ctggcggctg aagtcgatga acgcggaggg cgtcgccatg
     2161 acggtgacac cgcccgcgag cgccagcgag cactcaccgg aacgcagcgc ctgagcggcc
     2221 aggtgcagcg cgacgaggga cgacgagcaa gcggtgtcca ccgtcatcgc gggcccttcc
     2281 atcccgaaca cgtaggacac gcggccggac gcgacactgc ccgcgttgcc ggtgccgagg
     2341 aagctctcga caccttccgg cacgccgtac agctgcgagg cgtagtcgtg gtacatgacg
     2401 cccgcgaaca cgccggtggc gctgccccgc agcgactgcg ggtcgatgcc cgcccgctcg
     2461 aaggcctccc aggaggtttc gaggagcagt cgctgctgcg ggtccatggc cagggcctcg
     2521 cgcggcgaga tgccgaagaa gcccgggtcg aaccggtccg cgtcgtgcag gaagccgccc
     2581 tcgcgggtgt aggagctgcc gacccgctca cccgcggggt cgtacagcgc ctccacgtcc
     2641 cagccgcgct cggtcgggaa cggcgtgatg ccctcggccc cgtcgacgag cagtcgccac
     2701 aggtcctccg gcgtgttcac gccgccgggg tagcggcagc tcatgccgac gatcgcgatc
     2761 ggctcgtcgg tcgcggtcac ggccgcgacg gtggccggcg cggcgacgcg ctcgcccgcg
     2821 atctcgacgc cgacgaacgc ggcgatcgcc gcgggggtcg ggtagtcgaa gatcagcgtg
     2881 gcgggcaggc gaaggcccgt ggccgcgttc aggcggttgc gcaggtcgac ggcggtcagc
     2941 gagtcgaagc ccagctccgt gaacgcctgc tcgggcgcga ccgcctccgg cccggcgtgg
     3001 ccgagcaccg tggcggcctg ggtgcggacg aggtccagga gcgtccgggc gcgctcgtcc
     3061 tcggccagcg gggccagcag ggcgcggagc gccgcggccg ggtcggcccc ggtgcccgcg
     3121 gcccggcggg tggtcgtgcg gacgaggccg ctgaacaccg ggggtatttg cgggagttcg
     3181 cgtacggcgc gcaggtcgag ccgcatcggt acgacgaccg gtgcagtccc ggagaccgcg
     3241 gcgtcgaaca gcgcgacgcc ctcctcggcg gacaggccgg tcgcggcgga ctgcgccacg
     3301 cgggcgccca tgccgtcggc ccacaggccc cacgccagcg aggtcgcggg gagcccggcg
     3361 gcgcggcgcc gctgggcgag ggcgtccagg aaggcgttgg ccgccgagta gttgccctgt
     3421 ccgggtccgc cgagcgtgcc cgccaccgag gagaacagaa cgaacgccgt caggtccgac
     3481 tcacgggtca gctcgtgcag gtgccaggcc gcgtcgacct tcggccggaa gaccgtgtcg
     3541 acctggtcgg gcgtgagcga gccgatggtg ccgtcgtcga ggacaccggc ggtgtgcacg
     3601 acggcggtga cggggtgctc ggcgagcagc gccgccagcg cgtcccggtc cgcgacatcg
     3661 caggccgcgt acgtcacctc ggcgccctgc gcggtcagtt cggcgcgcag ttcctcggcg
     3721 ccctcggccg cggcgccgcg tcggctggcc aggacgaggc gtccgactcc gtgactgtcg
     3781 acgaggtgcc gggcgacgag accgcccagc gcacccgacg cgcccgtcac cagcaccgag
     3841 ctgtccggcc cgaacgccgg gtccgtgccg ggctccggcc gcagccgcgc gaggcgggcc
     3901 acgtgggccc ggccgtcccg cacgacgatc tggggttcgc cgctcgtcag gaccggcttc
     3961 caggtgttct cgtcgccgcc gtcgacatcg ccgtcgacat cgacgagcac gatgcggtcc
     4021 gggttctccg cctgtgccgt acgcaccagg ccccacaccg ccgacgccgc caggtccggc
     4081 acgtcctcgc cgggttcgac cgccaccgcg ccacgggtca ccaggacaag gcgcgagtcc
     4141 atgcaccggt catcggccag ccagccccgc accagctcca gggcggccag gacctgcggg
     4201 cgtaccgagg ccacgtcagc ggactcggcg caccgcacca cgacgttgtc cggcagcgac
     4261 tcgcccgcgt ccagctccga ccgcaccgcg gcccagtccg cgaactccgc caccggacca
     4321 gcggtcccgg ccgacgacgt cgccgtctga gcgggcaccc agtcgaggtg gaacagcgac
     4381 tcccagccct gccgcgacag cgcggccgac actggacgca gcac
//
