LOCUS       NZ_BMVR01000050         4768 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces flavofungini strain JCM 4753 sequence50, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVR01000050
VERSION     NZ_BMVR01000050.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces flavofungini
  ORGANISM  Streptomyces flavofungini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650815.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:46:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,873
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,638
            CDSs (with protein)               :: 7,638
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 43 of 161
            Pseudo Genes (incomplete)         :: 137 of 161
            Pseudo Genes (internal stop)      :: 10 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4768
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVR01000050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..4766)
                     /NRPS_PKS="Domain: PKS_KR (319-495). E-value: 8.2e-60.
                     Score: 193.8. Matches aSDomain:
                     nrpspksdomains_IE274_RS39010_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (600-673). E-value: 3.6e-31. Score:
                     99.3. Matches aSDomain: nrpspksdomains_IE274_RS39010_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (693-1118). E-value:
                     2.2e-186. Score: 611.8. Matches aSDomain:
                     nrpspksdomains_IE274_RS39010_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (1223-1507). E-value: 5.9e-106.
                     Score: 346.1. Matches aSDomain:
                     nrpspksdomains_IE274_RS39010_PKS_AT.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981856.1"
                     /locus_tag="IE274_RS39010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_190120698.1"
                     /sec_met_domain="adh_short (E-value: 6.2e-65, bitscore:
                     204.6, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.6e-09,
                     bitscore: 23.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.9e-15, bitscore:
                     41.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 1.2e-238, bitscore:
                     778.1, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 4.9e-98, bitscore:
                     313.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 9.8e-108, bitscore:
                     346.1, seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWPPAGAEVVDLEGFYDERADAGLAYGPVFQGLRAAWRKDGEVFA
                     EVALPDGVKSDGFGLHPALLDATLHTVGLADSADVDGKVPFAWSGVRLHASGATALRVR
                     LAPAGADAVTLTLADGAGAPVATVDSLVLRPVAETLSRQDGESLFGVDWVSVPLSDGDA
                     PTVAWADLEALTEPQSAELPGFVVLSCPDTSASDPAVGAHDAAHWALNAVQSWLTDERF
                     DAARLAVVTRGAIAASADEAVRDVAQAAVWGLVRSAQSENPDRIVLVDLDEETASLDAL
                     PSALATGEPQLALRSGTASAPRLARVSPSVVDDPGFGPGAVLVTGATGTLGGLMARHLV
                     AERGVRNLLLVSRRGAAAEGAEELRAELAAQGAEVVLAACDVAEREAVAALLAAHDISA
                     VVHTAGVLDDGVISSLTPERIDTVFRPKVDAAWHLHELTRDLNLSAFVLFSSVSGTLGA
                     PGQGNYAAANAFLDALAQHRRDAGLPATSLAWGLWAEASGMTGDLGEADVKRMGRGGLL
                     PLRTAEGLALFDAAGSTGRAALVPVPLDLAVLRREARTRPVPHLLRGLVRGTARRTAES
                     GPGASSALARSLAGMSVADREKALLDLVLGHVAVVLGHSSAGAIAPDRAFKDLGFDSLS
                     SVELRNHLGAATELALPATLVFDYPTPAALAAHLGAELAGATVVETVTGTTVAMADEPI
                     AIVGMSCRFPGGVESPEDLWRLVASGADALSGFPTDRGWDVESLYDPDPDHAGTTYARE
                     GGFLHDVGEFDADFFGISPREALAMDPQQRLLLETSWEAFERAGIDPHAVRGSRSGVFV
                     GTNGQDYAALLMAAQESAEGYAGTGSAASVISGRLSYTFGLEGPAVTVDTACSSSLVAL
                     HLAGQALNKGECELALVGGVTVMSTPGAFIEFSRQRGLAPDGRCKAFAEGADGTGWGEG
                     VGMLLVERLSDARRNGHEVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRQALASAGLA
                     AADVDAVEAHGTGTSLGDPIEAQALLATYGQDRGGDPLWLGSVKSNVGHTQAAAGVAGV
                     IKMVMAMREGVLPRTLHVDEPSTHVDWSAGAVRLLTETQPWPETGRPWRAGVSSFGVSG
                     TNAHAIIEQAPAVQELGVDAAPEPDGLAPWVLSARSAEALREQARRLKSFVDATRELAV
                     SDVGRALVSSRAVLEHRAVVVADDRDEFLAALAAVADSQASGAVVSGVADEPGRVGFLF
                     SGQGSQRLGMGRELSERFPVFAAVLDEVLAEFDPSVREVLFGQDADALNETGVTQPALF
                     AVEVALFRLLESWGVRPDMLAGHSIGELAAAHVSGVWSLPDAVKVVSARGALMQALPEG
                     GAMVAIQATEAEVAADELPDTVDIAAVNGPASVVVSGVAADVEAVAERWREAGRKATRL
                     KVSHAFHSPLMDPMLDEFKRVLAGVSYEAPQIPIVSTLTGRPATAEELASPDYWVRHVR
                     KAVRFADAVSALVAEGVATFVEVGPGGALSALGQESAPDAAFVPVLRGDRPEAVAATTA
                     AGHLHVRGVQVDWQPFFDGSGTRRVDLPTYAFQRERYWLDATYTTGDAAAIGLGSAEHP
                     LLGAAVAVGG"
     source          1..4768
                     /culture_collection="JCM:4753"
                     /db_xref="taxon:68200"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces flavofungini"
                     /strain="JCM 4753"
                     /submitter_seqid="sequence50"
                     /type_material="type strain of Streptomyces flavofungini"
     gene            complement(<1..>4768)
                     /locus_tag="IE274_RS39010"
     region          1..4768
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4768
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4768
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:4766](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..4766)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        complement(245..1096)
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS39010_PKS_AT.1"
                     /evalue="5.90E-106"
                     /label="IE274_RS39010_PKS_AT.1"
                     /locus_tag="IE274_RS39010"
                     /protein_end="1507"
                     /protein_start="1223"
                     /score="346.1"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFSGQGSQRLGMGRELSERFPVFAAVLDEVLAEFDPSVREVLFGQ
                     DADALNETGVTQPALFAVEVALFRLLESWGVRPDMLAGHSIGELAAAHVSGVWSLPDAV
                     KVVSARGALMQALPEGGAMVAIQATEAEVAADELPDTVDIAAVNGPASVVVSGVAADVE
                     AVAERWREAGRKATRLKVSHAFHSPLMDPMLDEFKRVLAGVSYEAPQIPIVSTLTGRPA
                     TAEELASPDYWVRHVRKAVRFADAVSALVAEGVATFVEVGPGGALSALGQESAPDAAFV
                     PVL"
     aSModule        complement(245..2686)
                     /domains="nrpspksdomains_IE274_RS39010_PKS_KS.1"
                     /domains="nrpspksdomains_IE274_RS39010_PKS_AT.1"
                     /incomplete
                     /locus_tags="IE274_RS39010"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(368..403)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0011"
                     /evalue="1.80E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IE274_RS39010"
                     /protein_end="1466"
                     /protein_start="1454"
                     /score="14.9"
                     /tool="antismash"
                     /translation="YWVRHVRKAVRF"
     CDS_motif       complement(626..664)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0010"
                     /evalue="7.50E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE274_RS39010"
                     /protein_end="1380"
                     /protein_start="1367"
                     /score="12.8"
                     /tool="antismash"
                     /translation="AAVNGPASVVVSG"
     CDS_motif       complement(752..874)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0009"
                     /evalue="4.90E-21"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="IE274_RS39010"
                     /protein_end="1338"
                     /protein_start="1297"
                     /score="67.0"
                     /tool="antismash"
                     /translation="GVRPDMLAGHSIGELAAAHVSGVWSLPDAVKVVSARGALMQ"
     CDS_motif       complement(884..943)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0008"
                     /evalue="1.80E-04"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE274_RS39010"
                     /protein_end="1294"
                     /protein_start="1274"
                     /score="14.7"
                     /tool="antismash"
                     /translation="ETGVTQPALFAVEVALFRLL"
     CDS_motif       complement(1049..1099)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0007"
                     /evalue="3.30E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE274_RS39010"
                     /protein_end="1239"
                     /protein_start="1222"
                     /score="16.4"
                     /tool="antismash"
                     /translation="FLFSGQGSQRLGMGREL"
     aSDomain        complement(1412..2686)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS39010_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="2.20E-186"
                     /label="IE274_RS39010_PKS_KS.1"
                     /locus_tag="IE274_RS39010"
                     /protein_end="1118"
                     /protein_start="693"
                     /score="611.8"
                     /tool="antismash"
                     /translation="IAIVGMSCRFPGGVESPEDLWRLVASGADALSGFPTDRGWDVESL
                     YDPDPDHAGTTYAREGGFLHDVGEFDADFFGISPREALAMDPQQRLLLETSWEAFERAG
                     IDPHAVRGSRSGVFVGTNGQDYAALLMAAQESAEGYAGTGSAASVISGRLSYTFGLEGP
                     AVTVDTACSSSLVALHLAGQALNKGECELALVGGVTVMSTPGAFIEFSRQRGLAPDGRC
                     KAFAEGADGTGWGEGVGMLLVERLSDARRNGHEVLAVVRGSAVNQDGASNGLTAPNGPS
                     QQRVIRQALASAGLAAADVDAVEAHGTGTSLGDPIEAQALLATYGQDRGGDPLWLGSVK
                     SNVGHTQAAAGVAGVIKMVMAMREGVLPRTLHVDEPSTHVDWSAGAVRLLTETQPWPET
                     GRPWRAGVSSFGVSGTNAHAIIEQAP"
     CDS_motif       complement(1634..1678)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0006"
                     /evalue="9.20E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE274_RS39010"
                     /protein_end="1044"
                     /protein_start="1029"
                     /score="11.7"
                     /tool="antismash"
                     /translation="GSVKSNVGHTQAAAG"
     CDS_motif       complement(1736..1780)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0005"
                     /evalue="3.60E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE274_RS39010"
                     /protein_end="1010"
                     /protein_start="995"
                     /score="12.7"
                     /tool="antismash"
                     /translation="VEAHGTGTSLGDPIE"
     CDS_motif       complement(2156..2203)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0004"
                     /evalue="7.70E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE274_RS39010"
                     /protein_end="870"
                     /protein_start="854"
                     /score="20.9"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     aSDomain        complement(2747..2965)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS39010_ACP.1"
                     /evalue="3.60E-31"
                     /label="IE274_RS39010_ACP.1"
                     /locus_tag="IE274_RS39010"
                     /protein_end="673"
                     /protein_start="600"
                     /score="99.3"
                     /tool="antismash"
                     /translation="LDLVLGHVAVVLGHSSAGAIAPDRAFKDLGFDSLSSVELRNHLGA
                     ATELALPATLVFDYPTPAALAAHLGAEL"
     aSModule        complement(2747..3808)
                     /domains="nrpspksdomains_IE274_RS39010_PKS_KR.1"
                     /domains="nrpspksdomains_IE274_RS39010_ACP.1"
                     /incomplete
                     /locus_tags="IE274_RS39010"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(3281..3808)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS39010_PKS_KR.1"
                     /evalue="8.20E-60"
                     /label="IE274_RS39010_PKS_KR.1"
                     /locus_tag="IE274_RS39010"
                     /protein_end="495"
                     /protein_start="319"
                     /score="193.8"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GAVLVTGATGTLGGLMARHLVAERGVRNLLLVSRRGAAAEGAEEL
                     RAELAAQGAEVVLAACDVAEREAVAALLAAHDISAVVHTAGVLDDGVISSLTPERIDTV
                     FRPKVDAAWHLHELTRDLNLSAFVLFSSVSGTLGAPGQGNYAAANAFLDALAQHRRDAG
                     LPATSLAWGLWAE"
     CDS_motif       complement(3329..3433)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0003"
                     /evalue="8.70E-22"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE274_RS39010"
                     /protein_end="479"
                     /protein_start="444"
                     /score="69.8"
                     /tool="antismash"
                     /translation="AFVLFSSVSGTLGAPGQGNYAAANAFLDALAQHRR"
     CDS_motif       complement(3461..3490)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0002"
                     /evalue="6.70E-03"
                     /label="PKSI-KR_m3"
                     /locus_tag="IE274_RS39010"
                     /protein_end="435"
                     /protein_start="425"
                     /score="10.0"
                     /tool="antismash"
                     /translation="PKVDAAWHLH"
     CDS_motif       complement(3704..3802)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS39010_0001"
                     /evalue="9.70E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE274_RS39010"
                     /protein_end="354"
                     /protein_start="321"
                     /score="24.6"
                     /tool="antismash"
                     /translation="VLVTGATGTLGGLMARHLVAERGVRNLLLVSRR"
ORIGIN
        1 gccacccaca gcaacagccg cacccaacaa cggatgctcc gccgaaccga gcccgatcgc
       61 cgcggcatca ccggtcgtgt atgtggcgtc cagccagtag cgctcgcgct ggaacgcgta
      121 cgtcggcagg tccacccgtc gcgtaccgct gccgtcgaag aagggctgcc agtcgacctg
      181 gacaccgcgc acgtgcaggt gcccggcagc cgtcgtggcg gccacggcct ccggccgatc
      241 gccccgcagc acgggtacga aggccgcgtc cggcgcggac tcctgcccca gcgccgacag
      301 cgccccgccg ggaccgacct cgacgaacgt cgccacgccc tcggcgacca gagcgctcac
      361 ggcgtcggcg aagcgcaccg ccttccgcac gtgccgcacc cagtagtccg gggaggccag
      421 ctcctcggcg gtggccggtc ggccggtgag tgtcgacacg atcgggatct ggggcgcctc
      481 gtacgacacg cccgcgagga cccgcttgaa ctcgtccagc atcgggtcca tcagcggcga
      541 gtggaacgcg tggctgacct tcagccgggt cgccttgcga cccgcctcac gccaccgctc
      601 cgcgaccgcc tcgacatcag cggccacacc cgaaacaacc accgaggcgg ggccgttcac
      661 ggcagcgatg tcgacggtgt ccggcagttc gtcggccgcg acctcggcct ccgtcgcctg
      721 gatcgcgacc atcgcgccgc cctcggggag cgcctgcatc agcgcacccc gcgcggacac
      781 caccttgacg gcatccggca gcgaccagac acccgacaca tgcgcggcgg ccagctcacc
      841 gatcgagtgg cccgccagca tgtcggggcg gacgccccac gactcaagga gccggaacag
      901 cgccacctcg accgcgaaca acgcgggctg cgtcaccccg gtctcattca gggcgtccgc
      961 gtcctgaccg aacaacacct cacgcaccga cggatcgaac tccgcaagga cctcgtcaag
     1021 caccgccgcg aacaccggga aacgctcgga cagttcacgc cccataccaa ggcgctgcga
     1081 accctgaccc gagaacagga aacccacccg gccgggctca tccgcgacgc ccgagaccac
     1141 ggcgccgctc gcctggctgt cggccaccgc cgccagagca gccaggaact cgtcccggtc
     1201 atcggcgacg accaccgcac ggtgctcgag caccgcccgc gacgacacca gcgcacgacc
     1261 cacgtccgac acggccagct cacgcgtcgc gtccacgaag gacttcagac gcctggcctg
     1321 ctcccgcagc gcctcggcgc tccgggccga aaggacccac ggcgcgagcc cgtcgggctc
     1381 cggcgccgcg tcgacgccca actcctggac ggccggggcc tgttcgatga tggcgtgcgc
     1441 gttcgtgccg ctcacgccga acgacgacac acccgcccgc cacggccgac ccgtctcggg
     1501 ccacggctgg gtctccgtca ggagccggac ggcgcccgcg gaccagtcga cgtgcgtcga
     1561 gggctcgtcg acgtgcaggg tgcgcggcag cacgccctcc cgcatcgcca tgaccatctt
     1621 gatcacaccc gcgacaccgg ccgcggcctg cgtgtgaccg acgttcgact tgaccgagcc
     1681 gagccacagc gggtcaccgc cgcggtcctg cccgtacgtg gcgagcaggg cctgcgcctc
     1741 gatcgggtcg cccagcgacg tgccggtgcc gtgcgcctcg accgcgtcca cgtcggccgc
     1801 ggccaggccc gcgctggcca gtgcctggcg gatgacgcgc tgctgcgacg ggccgttcgg
     1861 ggccgtcagg ccgttcgacg cgccgtcctg gttcaccgcg gagccgcgca cgaccgcaag
     1921 cacctcgtgg ccgttgcgcc gggcgtcgga aaggcgctcc acgagcagca tgcccacgcc
     1981 ctcgccccag ccggtgccgt ccgcgccctc ggcgaacgcc ttgcagcggc cgtccggcgc
     2041 gaggccgcgc tggcggctga actcgatgaa cgcacccggc gtcgacatca ccgtcacacc
     2101 gccgaccagg gccagctcgc actcgccctt gttcagcgcc tgacccgcca ggtgcagggc
     2161 gaccagcgac gaggagcacg ccgtgtccac agtgacggcc gggccttcga ggccgaacgt
     2221 gtacgagagg cggcccgaga tgacgctcgc cgcgctgccc gtgcccgcgt agccctcggc
     2281 gctctcctgc gcggccatca acagcgccgc gtagtcctgg ccgttggtgc cgacgaacac
     2341 accggagcgg ctgccgcgta ccgcgtgcgg gtcgatgccc gcccgctcga acgcctccca
     2401 ggaggtttcg aggagcagtc gctgctgcgg gtccatggcg agggcctcgc gcggcgagat
     2461 gccgaagaag tccgcgtcga actcgccgac gtcgtgcagg aagccgccct cacgggcgta
     2521 ggtcgtgccc gcgtggtcgg ggtccgggtc gtagagcgac tccacgtccc agccgcggtc
     2581 ggtcgggaag ccggacagtg cgtccgcgcc ggacgcgacg agccgccaca ggtcctcggg
     2641 cgactcgacg ccgccgggga agcggcagct catgccgacg atcgcgatcg gttcgtcggc
     2701 catggcgacc gtcgtgccgg tgaccgtttc gaccacggtg gcgcccgcga gttcggcgcc
     2761 gaggtgggcg gcgagtgcgg cgggggtcgg gtagtcgaag acgagcgtgg cgggcagggc
     2821 cagttcggtc gccgcgccca ggtggttacg cagttcgacg gagctgagcg agtcgaagcc
     2881 gaggtccttg aacgcgcggt cgggtgcgat ggcgcccgcc gagctgtggc cgagcaccac
     2941 ggccacgtga ccgagtacga ggtcgaggag ggccttctcc cggtccgcga cgctcatccc
     3001 ggcgagcgag cgggccagcg ccgaactcgc gccggggccg gactcggcgg tcctgcgcgc
     3061 ggtaccgcgc accaggccgc gcaggaggtg cgggacgggg cgggtgcggg cctcgcggcg
     3121 cagcacggcc aggtccaggg gcaccggtac gagcgcggcg cggccggtcg agcccgccgc
     3181 gtcgaacagg gcgaggccct cggcggtgcg gagcgggagc aggccgcccc ggcccatccg
     3241 cttcacgtcg gcctcgccga ggtccccggt catgccgctg gcctcggccc acagacccca
     3301 cgcgagcgag gtggcgggca gtccggcatc acggcggtgc tgcgcgagcg cgtccaggaa
     3361 cgcgttcgcc gccgcgtagt tgccctggcc gggggcgccc agcgtgcccg agaccgagga
     3421 gaacaggacg aacgcggaca ggttcaggtc gcgggtcagc tcgtgcaggt gccaggccgc
     3481 gtcgaccttc gggcggaaga cggtgtcgat ccgctccggg gtgagcgagc tgatcacgcc
     3541 gtcgtcgagg acaccggcgg tgtggacgac ggccgagatg tcgtgcgcgg ccagcagcgc
     3601 ggcgacagcc tcgcgctcgg ccacatcgca ggcggcgagc actacttcgg cgccctgcgc
     3661 ggcgagttcg gcgcgcagtt cctcggcgcc ttcggcggcg gcgccgcgcc tgctcaccag
     3721 cagcaggttg cgtacgccgc gctcggcgac caggtgccgg gccatgaggc cgcccagcgt
     3781 gcccgtggca ccggtgacca gcaccgcacc cggcccgaag ccggggtcgt ccaccacgga
     3841 cgggctgacc ctggccaggc gcggggcgct cgcggtgccc gagcggagcg ccaactgcgg
     3901 ctcaccggtg gccagcgcgg acggcagcgc gtccagcgag gccgtctcct cgtcgaggtc
     3961 gaccaggacg atccggtccg ggttctccga ctgggcggac cgcaccaggc cccacacggc
     4021 ggcctgcgcg acgtcccgta ccgcctcatc ggccgaggcg gcgatcgcgc ccctggtcac
     4081 gacggccagg cgggccgcgt cgaagcgctc gtcggtcagc cagctctgga ccgcgttcag
     4141 cgcccagtgc gcggcgtcgt gcgcgccgac ggccgggtcg gacgccgagg tgtcggggca
     4201 cgacaggacc acgaagcccg gcagttccgc cgactgcggc tcggtcagtg cttccaggtc
     4261 ggcccatgcg accgtcgggg cgtcgccgtc ggacaggggc acggacaccc agtcgacgcc
     4321 gaacagcgat tccccgtcct gcctcgacag cgtctccgct acgggacgca ggacgaggga
     4381 gtccaccgtc gcgacggggg cgcccgcgcc gtcggcgagc gtcagcgtca ccgcgtccgc
     4441 tccggcaggg gcgaggcgca cgcgcagtgc cgtcgcgccg gaggcgtgca gccgcacacc
     4501 cgaccacgcg aacggcacct tgccgtcgac gtccgcgctg tccgcgagac cgaccgtgtg
     4561 caacgtcgcg tccagcagcg ccggatgcag accgaagccg tccgacttca caccatcggg
     4621 caacgcgacc tcggcgaaca cctcgccgtc cttgcgccac gcggcccgca agccctggaa
     4681 caccggcccg taggcgagac cggcgtcagc cctctcgtcg tagaacccct ccagatccac
     4741 aacctcagcc ccagccggag gccacacc
//
