LOCUS       NZ_BMVR01000048         5813 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces flavofungini strain JCM 4753 sequence48, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVR01000048
VERSION     NZ_BMVR01000048.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces flavofungini
  ORGANISM  Streptomyces flavofungini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650815.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:46:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,873
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,638
            CDSs (with protein)               :: 7,638
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 43 of 161
            Pseudo Genes (incomplete)         :: 137 of 161
            Pseudo Genes (internal stop)      :: 10 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5813
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVR01000048.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..3272
                     /locus_tag="IE274_RS38975"
     source          1..5813
                     /culture_collection="JCM:4753"
                     /db_xref="taxon:68200"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces flavofungini"
                     /strain="JCM 4753"
                     /submitter_seqid="sequence48"
                     /type_material="type strain of Streptomyces flavofungini"
     region          1..5813
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..5813
                     /SMILES="CC(O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5813
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3541:>5813](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3542..>5813
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             <5..3272
                     /NRPS_PKS="Domain: PKS_AT (3-255). E-value: 1e-91. Score:
                     299.4. Matches aSDomain:
                     nrpspksdomains_IE274_RS38975_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (631-809). E-value: 6e-67. Score:
                     217.1. Matches aSDomain:
                     nrpspksdomains_IE274_RS38975_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (910-996). E-value: 4.6e-33.
                     Score: 105.4. Matches aSDomain:
                     nrpspksdomains_IE274_RS38975_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (1007-1088). E-value:
                     1.8e-14. Score: 46.3. Matches aSDomain:
                     nrpspksdomains_IE274_RS38975_PKS_Docking_Cterm.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012901.5"
                     /locus_tag="IE274_RS38975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_190120692.1"
                     /sec_met_domain="PKS_AT (E-value: 3.4e-93, bitscore: 299.4,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 8.1e-67, bitscore:
                     211.7, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.4e-16, bitscore:
                     46.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LQDVMFGADAELLEQTGYAQPALFAVEVALFRLAESFGVRPEVVG
                     GHSIGELTAAYVAGLWSLEDAARLVAARGRLMQALPVGGAMLAVQAAEADVLPLLEGVS
                     DLVGLAAVNGPSQIVLSGERAALEGLEETLRGQGRKVRWLKVSHAFHSPLMDPVLEDFR
                     QVAEKLTYQDTVLPVVSNLTGQLATPDELKDPGYWVRHVREAVRFHDGLGTLSGQGVST
                     LLELGPDAVLTAMAHDTLTDPAAQSGLIAALRKDRPEPDTFLTALAKVQVRGADTDWAP
                     LYAPAEARRRVDLPTYAFQHEHYWLEAASDTAGPAGSGAVDEVESRFWETVESADLAEL
                     AKTLEVPDDAPLTEVLPALTAWRKQRRETFTLDQWNYRESWTPLTESAAGKLTGTWLVV
                     VPEGRADGQTNGPADGTPDASWRAAVAALMAEHDVRLVTVEVPAHATARDALAGPLRAA
                     LGESADVGAFDGVLSLLAVADGADDSAADAGTPHAGGPSDGTGASGGTDPVPAAVALTL
                     ALIQALGDTGVDAPLWCATRDAVAVGHADAGTAPGRTALWGLGRVAALEYPERWGGLVD
                     LPDAADTAAASRLGLVLGGAWAGEDQVAVRGSGVFGRRVVRAPHDGGSAAEWTPHGTVL
                     VTGGTGALGSHVARWLAREGADHVVLTSRRGADAPGAAELRGELEESGVRVTVAACDAA
                     DRDALARLLGDIPDDAPLTGVFHAAGVLDDGVLDAMTPERLATVFRAKTESARNLDELT
                     AHLDLSAFVLFSSFAGVAGGAGQGSYAAANAYLDGLAHARRARGLTATSVAWGPWAGSG
                     MAAEGVAAERLRGGALPPLEPERAIAALQRLLAQDRTAVLVADVDWQQYAPAFTAVRPS
                     RLYAELPEAEAARRSGTGIADTGTGAAAAGPGGPDLAARLASLARPEQDRLLLDLVCEQ
                     VAAVLGYSGAAAIEPTRAFRELGFDSLTAVELRNRVNALTGLVLPATVVFDHPTPTALA
                     DCLRTELAPDAADPALLLLGDLDRLEGSLAAAGTADEITRTKVAVRLQAALDRWKNTAT
                     GTGGQPGGNDPAADAPDVGEQLAAASDDEMLEFIKKQLGRG"
     aSDomain        15..770
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38975_PKS_AT.1"
                     /evalue="1.00E-91"
                     /label="IE274_RS38975_PKS_AT.1"
                     /locus_tag="IE274_RS38975"
                     /protein_end="255"
                     /protein_start="3"
                     /score="299.4"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="VMFGADAELLEQTGYAQPALFAVEVALFRLAESFGVRPEVVGGHS
                     IGELTAAYVAGLWSLEDAARLVAARGRLMQALPVGGAMLAVQAAEADVLPLLEGVSDLV
                     GLAAVNGPSQIVLSGERAALEGLEETLRGQGRKVRWLKVSHAFHSPLMDPVLEDFRQVA
                     EKLTYQDTVLPVVSNLTGQLATPDELKDPGYWVRHVREAVRFHDGLGTLSGQGVSTLLE
                     LGPDAVLTAMAHDTLTDPAAQSGLIAALRK"
     aSModule        15..2993
                     /complete
                     /domains="nrpspksdomains_IE274_RS38975_PKS_AT.1"
                     /domains="nrpspksdomains_IE274_RS38975_PKS_KR.1"
                     /domains="nrpspksdomains_IE274_RS38975_PKS_PP.1"
                     /locus_tags="IE274_RS38975"
                     /monomer_pairings="mal -> ohmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     CDS_motif       54..104
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38975_0001"
                     /evalue="5.90E-04"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE274_RS38975"
                     /protein_end="33"
                     /protein_start="16"
                     /score="13.1"
                     /tool="antismash"
                     /translation="GYAQPALFAVEVALFRL"
     CDS_motif       117..239
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38975_0002"
                     /evalue="1.20E-22"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="IE274_RS38975"
                     /protein_end="78"
                     /protein_start="37"
                     /score="72.1"
                     /tool="antismash"
                     /translation="GVRPEVVGGHSIGELTAAYVAGLWSLEDAARLVAARGRLMQ"
     CDS_motif       333..371
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38975_0003"
                     /evalue="2.20E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE274_RS38975"
                     /protein_end="122"
                     /protein_start="109"
                     /score="14.4"
                     /tool="antismash"
                     /translation="AAVNGPSQIVLSG"
     CDS_motif       594..629
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38975_0004"
                     /evalue="4.20E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IE274_RS38975"
                     /protein_end="208"
                     /protein_start="196"
                     /score="16.8"
                     /tool="antismash"
                     /translation="YWVRHVREAVRF"
     CDS_motif       1089..1112
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38975_0005"
                     /evalue="3.30E+01"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IE274_RS38975"
                     /protein_end="369"
                     /protein_start="361"
                     /score="-1.2"
                     /tool="antismash"
                     /translation="WRKQRRET"
     aSDomain        1899..2432
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: True"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38975_PKS_KR.1"
                     /evalue="6.00E-67"
                     /label="IE274_RS38975_PKS_KR.1"
                     /locus_tag="IE274_RS38975"
                     /protein_end="809"
                     /protein_start="631"
                     /score="217.1"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLVTGGTGALGSHVARWLAREGADHVVLTSRRGADAPGAAELR
                     GELEESGVRVTVAACDAADRDALARLLGDIPDDAPLTGVFHAAGVLDDGVLDAMTPERL
                     ATVFRAKTESARNLDELTAHLDLSAFVLFSSFAGVAGGAGQGSYAAANAYLDGLAHARR
                     ARGLTATSVAWGPWA"
     CDS_motif       1902..2000
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38975_0006"
                     /evalue="1.00E-14"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE274_RS38975"
                     /protein_end="665"
                     /protein_start="632"
                     /score="47.0"
                     /tool="antismash"
                     /translation="TVLVTGGTGALGSHVARWLAREGADHVVLTSRR"
     CDS_motif       2283..2387
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38975_0007"
                     /evalue="8.00E-17"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE274_RS38975"
                     /protein_end="794"
                     /protein_start="759"
                     /score="53.8"
                     /tool="antismash"
                     /translation="AFVLFSSFAGVAGGAGQGSYAAANAYLDGLAHARR"
     aSDomain        2736..2993
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38975_PKS_PP.1"
                     /evalue="4.60E-33"
                     /label="IE274_RS38975_PKS_PP.1"
                     /locus_tag="IE274_RS38975"
                     /protein_end="996"
                     /protein_start="910"
                     /score="105.4"
                     /tool="antismash"
                     /translation="LASLARPEQDRLLLDLVCEQVAAVLGYSGAAAIEPTRAFRELGFD
                     SLTAVELRNRVNALTGLVLPATVVFDHPTPTALADCLRTEL"
     aSDomain        3027..3269
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38975_PKS_Docking_Cterm.
                     1"
                     /evalue="1.80E-14"
                     /label="IE274_RS38975_PKS_Docking_Cterm.1"
                     /locus_tag="IE274_RS38975"
                     /protein_end="1088"
                     /protein_start="1007"
                     /score="46.3"
                     /tool="antismash"
                     /translation="LGDLDRLEGSLAAAGTADEITRTKVAVRLQAALDRWKNTATGTGG
                     QPGGNDPAADAPDVGEQLAAASDDEMLEFIKKQLGR"
     misc_feature    3331..3344
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.31"
                     /tool="antismash"
     gene            3542..>5813
                     /locus_tag="IE274_RS38980"
     CDS             3542..>5813
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (3-31). E-value:
                     1.8e-15. Score: 48.2. Matches aSDomain:
                     nrpspksdomains_IE274_RS38980_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (35-465). E-value:
                     1.8e-181. Score: 595.6. Matches aSDomain:
                     nrpspksdomains_IE274_RS38980_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (580-756). E-value: 1.4e-68.
                     Score: 223.3. Matches aSDomain:
                     nrpspksdomains_IE274_RS38980_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_121825819.1"
                     /locus_tag="IE274_RS38980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_190120693.1"
                     /sec_met_domain="mod_KS (E-value: 9.9e-227, bitscore:
                     739.8, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 5e-97, bitscore:
                     311.5, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 4.8e-70, bitscore: 223.3,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANEETLRDYLKWVSADLHQTQQRLKDIEAAAQEPIAITAMSCRF
                     PGGVSGPEELWRLLSEGRDAISGLPTDRNWDLESLYDPDAAGKQGTTSTANGGFLDGVA
                     EFDAAFFGISPREALAMDPQQRLLLETAWEALERAGIDPATVRGSRTGVFVGTNGQDYA
                     PLLFEAEEDVEGYVSTGNAAAVESGRIAYTLGLEGPAVTVDTACSSSLVALHLAVQALR
                     QGECGMALVAGVTVISTPGVFTEFSRQQGLAADGRCKAFADAADGTGWGEGVGMLLVER
                     LSDARRAGRQVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIMQALANARLTAADIDAVE
                     AHGTGTTLGDPIEAQALLATYGQERPDSGEPLWLGSIKSNIGHTQAAAGVAGIIKMVMA
                     MRAGVLPQTLHVDEPTSQVNWSAGAVAVLTEARAWAEREGGAPRRAGVSSFGVSGTNAH
                     VIVEQAPGEETGAGEADADAAPSSSTVLPGVVPLALSGRTEGAVRAQAERVRELLRAQD
                     GLELGDVAWSLAVGRARFDQRGVVLGRDRDELLAGLEALAQDGPGAQNAVSGRALGGAV
                     RPVFVFPGQGSQWAGMARELLDSSPVFADRMRECGDALSEFVEWNLLDELSGDTFDQVD
                     VVQPVLFAVMVSLAATWQAAGVQPAAVVGHSQGEIAAACVAGALSLRDAARVVALRSLA
                     IRELSGKGGMVSVPLPEAEVRELIAAWDGRIELAAVNGPAQVVVSGEPEALEELVAQCV
                     AQDI"
     aSDomain        3551..3634
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38980_PKS_Docking_Nterm.
                     1"
                     /evalue="1.80E-15"
                     /label="IE274_RS38980_PKS_Docking_Nterm.1"
                     /locus_tag="IE274_RS38980"
                     /protein_end="31"
                     /protein_start="3"
                     /score="48.2"
                     /tool="antismash"
                     /translation="EETLRDYLKWVSADLHQTQQRLKDIEAA"
     aSDomain        3647..4936
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38980_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.80E-181"
                     /label="IE274_RS38980_PKS_KS.1"
                     /locus_tag="IE274_RS38980"
                     /protein_end="465"
                     /protein_start="35"
                     /score="595.6"
                     /tool="antismash"
                     /translation="IAITAMSCRFPGGVSGPEELWRLLSEGRDAISGLPTDRNWDLESL
                     YDPDAAGKQGTTSTANGGFLDGVAEFDAAFFGISPREALAMDPQQRLLLETAWEALERA
                     GIDPATVRGSRTGVFVGTNGQDYAPLLFEAEEDVEGYVSTGNAAAVESGRIAYTLGLEG
                     PAVTVDTACSSSLVALHLAVQALRQGECGMALVAGVTVISTPGVFTEFSRQQGLAADGR
                     CKAFADAADGTGWGEGVGMLLVERLSDARRAGRQVLAVVRGSAVNQDGASNGLTAPNGP
                     SQQRVIMQALANARLTAADIDAVEAHGTGTTLGDPIEAQALLATYGQERPDSGEPLWLG
                     SIKSNIGHTQAAAGVAGIIKMVMAMRAGVLPQTLHVDEPTSQVNWSAGAVAVLTEARAW
                     AEREGGAPRRAGVSSFGVSGTNAHVIVEQAP"
     aSModule        3647..5809
                     /domains="nrpspksdomains_IE274_RS38980_PKS_KS.1"
                     /domains="nrpspksdomains_IE274_RS38980_PKS_AT.1"
                     /incomplete
                     /locus_tags="IE274_RS38980"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       4133..4180
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0001"
                     /evalue="3.20E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE274_RS38980"
                     /protein_end="213"
                     /protein_start="197"
                     /score="22.1"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       4475..4495
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0002"
                     /evalue="8.70E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE274_RS38980"
                     /protein_end="318"
                     /protein_start="311"
                     /score="-2.6"
                     /tool="antismash"
                     /translation="APNGPSQ"
     CDS_motif       4556..4600
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0003"
                     /evalue="1.60E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE274_RS38980"
                     /protein_end="353"
                     /protein_start="338"
                     /score="13.7"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       4664..4708
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0004"
                     /evalue="3.50E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE274_RS38980"
                     /protein_end="389"
                     /protein_start="374"
                     /score="12.8"
                     /tool="antismash"
                     /translation="GSIKSNIGHTQAAAG"
     CDS_motif       4970..4996
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0005"
                     /evalue="4.30E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE274_RS38980"
                     /protein_end="485"
                     /protein_start="476"
                     /score="-1.9"
                     /tool="antismash"
                     /translation="DAAPSSSTV"
     CDS_motif       5219..5257
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0006"
                     /evalue="9.50E+01"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="IE274_RS38980"
                     /protein_end="572"
                     /protein_start="559"
                     /score="-2.7"
                     /tool="antismash"
                     /translation="GPGAQNAVSGRAL"
     CDS_motif       5276..5332
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0007"
                     /evalue="8.20E-11"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE274_RS38980"
                     /protein_end="597"
                     /protein_start="578"
                     /score="33.2"
                     /tool="antismash"
                     /translation="VFVFPGQGSQWAGMARELL"
     aSDomain        5282..5809
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38980_PKS_AT.1"
                     /evalue="1.40E-68"
                     /label="IE274_RS38980_PKS_AT.1"
                     /locus_tag="IE274_RS38980"
                     /protein_end="756"
                     /protein_start="580"
                     /score="223.3"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Ethylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFPGQGSQWAGMARELLDSSPVFADRMRECGDALSEFVEWNLLDE
                     LSGDTFDQVDVVQPVLFAVMVSLAATWQAAGVQPAAVVGHSQGEIAAACVAGALSLRDA
                     ARVVALRSLAIRELSGKGGMVSVPLPEAEVRELIAAWDGRIELAAVNGPAQVVVSGEPE
                     ALEELVAQCVAQD"
     CDS_motif       5435..5503
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0008"
                     /evalue="4.20E-11"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE274_RS38980"
                     /protein_end="654"
                     /protein_start="631"
                     /score="34.7"
                     /tool="antismash"
                     /translation="DQVDVVQPVLFAVMVSLAATWQA"
     CDS_motif       5507..5617
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0009"
                     /evalue="7.40E-20"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="IE274_RS38980"
                     /protein_end="692"
                     /protein_start="655"
                     /score="62.7"
                     /tool="antismash"
                     /translation="GVQPAAVVGHSQGEIAAACVAGALSLRDAARVVALRS"
     CDS_motif       5723..5761
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38980_0010"
                     /evalue="7.00E-05"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE274_RS38980"
                     /protein_end="740"
                     /protein_start="727"
                     /score="15.9"
                     /tool="antismash"
                     /translation="AAVNGPAQVVVSG"
ORIGIN
        1 cgctccagga cgtgatgttt ggtgcggatg ctgagctgtt ggagcagacg gggtacgcgc
       61 agcccgcgtt gttcgcggtc gaggtggcgc tgttccggct ggctgagtcc ttcggggtgc
      121 ggcctgaggt tgtcggcggc cactcgatcg gtgagctgac ggctgcgtac gtagcggggt
      181 tgtggtccct ggaggacgcc gcccgactgg tcgccgcgcg cggccggttg atgcaggctc
      241 ttccggtggg cggggccatg ctggccgtgc aggcggccga ggccgacgtg ctgccgctgc
      301 ttgagggtgt gtcggacctt gtcggccttg ccgccgtcaa cgggccttcg cagatcgtgc
      361 tctccgggga gcgggctgcg ctggagggcc tggaggagac cctgcgcggt cagggtcgca
      421 aggtccgctg gctgaaggtc tcccacgcct tccactcgcc gctcatggat ccggtcctgg
      481 aggacttccg ccaggtcgcc gagaagctga cgtaccagga caccgtcctg cccgtcgtct
      541 cgaacctgac cgggcagctc gccaccccgg atgagctgaa ggaccccggc tactgggtgc
      601 ggcacgtccg cgaggccgtc cggttccacg acggtctggg cacgctttcc ggccagggag
      661 tgtccacgct cctggagttg ggcccggacg ccgtcctgac ggcgatggcg cacgacacgc
      721 tcaccgaccc ggccgcgcag tccggcctga tcgccgccct gcgcaaggac cgccccgagc
      781 cggacacgtt cctgaccgcg ctcgccaagg tccaggtgcg cggcgcggac accgactggg
      841 cgccgctgta cgccccggcc gaggcgcggc gccgcgtcga cctgcccacg tacgccttcc
      901 agcacgagca ctactggctg gaggcggcgt cggacacggc gggcccggcg gggtccggtg
      961 cggtggacga ggtggagtcg cggttctggg agaccgtgga gagcgcggac ctggccgaac
     1021 tggccaagac cctcgaagtg ccggacgacg ccccgctcac cgaggtgctg cccgcgctga
     1081 cggcctggcg gaagcagcgg cgcgagacct tcaccctcga ccagtggaac taccgcgagt
     1141 cctggacgcc gctcaccgag tcggcggcgg ggaagctgac gggcacctgg ctcgtcgtcg
     1201 taccggaagg ccgggcggac gggcagacga atggcccggc ggacggcacc cctgacgcgt
     1261 cgtggcgcgc ggccgtggcg gccctgatgg ccgagcacga cgtgcggctc gtgacggtgg
     1321 aagtgcccgc gcacgccacc gcccgggacg cgctcgccgg accgctgcgg gcggcgctcg
     1381 gcgaatcggc cgacgtgggc gccttcgacg gcgtgctgtc gctgctggcc gtcgccgacg
     1441 gcgccgacga ctcggcagcc gacgcgggca ccccgcacgc gggcggcccg tccgacggca
     1501 ccggcgcctc cggtggcacc gaccccgtcc cggcggcagt cgcgctcacc ctggccctga
     1561 tccaggccct cggcgacacc ggcgtcgacg cacccctgtg gtgcgccacc cgtgacgccg
     1621 tcgccgtggg ccatgccgac gcggggaccg cgccgggccg gacggccctg tggggtctcg
     1681 gccgggtcgc cgcactggag tacccggagc gctggggcgg tctggtcgac ctgcccgacg
     1741 ccgccgacac ggccgccgcg tcccggctcg gcctggtcct cggtggcgcg tgggccggtg
     1801 aggaccaggt ggcggtgcgc gggtccgggg tgttcgggcg ccgcgtggtg cgggcgccgc
     1861 acgacggcgg atccgccgcc gagtggacgc cgcacggcac cgtgctcgtc accggcggca
     1921 ccggcgccct cggcagccac gtcgcgcgct ggctggcccg cgagggagcc gaccacgtcg
     1981 tcctcaccag caggcggggc gcggacgccc cgggcgcggc ggaactccgc ggcgaactgg
     2041 aggagtcggg cgtccgcgtg accgtcgcgg cctgcgacgc cgccgaccgg gacgcgctgg
     2101 cgcgcctcct cggggacatc cccgacgacg cgccactgac cggcgtgttc cacgcggccg
     2161 gtgtcctcga cgacggcgtg ctcgacgcga tgacacccga gcggctcgcg accgtgttcc
     2221 gcgcgaagac ggagtcggcc cgcaacctcg acgaactgac ggcccatctg gacctgtcgg
     2281 cgttcgtcct gttctcctcc ttcgccggtg tggccggcgg cgccggacag ggcagctacg
     2341 cggccgccaa cgcctacctc gacgggctcg cccacgcccg ccgggcccgg ggcctgaccg
     2401 ccacctccgt ggcgtggggc ccgtgggcgg gcagcggcat ggccgccgag ggagtggccg
     2461 cggaacggct gcggggcggg gcgctcccgc ccctggagcc ggagcgcgcg atcgccgcac
     2521 tccagcgcct cctcgcccag gaccgcaccg ccgtcctcgt cgccgacgtc gactggcagc
     2581 agtacgcacc ggccttcacc gccgtccggc ccagcaggct ctacgccgaa ctgcccgaag
     2641 cggaagcggc acgccggtcc ggcacgggaa tcgccgacac cggcaccggg gcagcggccg
     2701 cgggaccggg cggcccggac ctggcggcgc gcctcgcgtc gctggcgcgg cccgaacagg
     2761 accggctgct cctcgacctg gtctgcgaac aggtcgccgc cgtcctcggc tacagcggcg
     2821 ccgccgccat cgaacccacc cgcgccttcc gcgagctggg cttcgactcc ctcaccgccg
     2881 tcgaactccg caaccgcgtc aacgccctca ccggcctcgt cctgcccgcc accgtggtct
     2941 tcgaccaccc gacccccacg gccctcgccg actgcctgcg caccgaactc gccccggacg
     3001 ccgccgatcc cgccctgctc ctgctcggcg acctcgaccg gctcgaagga agcctcgcgg
     3061 ccgccggaac cgccgacgag atcacccgca ccaaggtcgc cgtgcgcctc caggcggcac
     3121 tcgaccgctg gaagaacacg gccacgggca cgggcgggca gccgggcgga aacgacccgg
     3181 ccgccgacgc ccctgacgtc ggcgaacaac tcgccgccgc cagcgacgac gagatgctgg
     3241 aattcatcaa gaagcaactc ggcaggggct gacaaccacc cagcgataaa ccgcgtcttc
     3301 gtcgaagtat ggacaaggag cgcccgtcaa ggtaattaat tgaaccgaca gcccctaccc
     3361 accccctttg ctcacgcgct cgcccccggc ctcaattcga ggcaatcccg taggggcgag
     3421 tagggggagg gtaggggttg ttggtcgtat ggggccccca tagcgtcatc gagaaaacgg
     3481 ctgcggacag gtccgttgta tgccagaggg tgcggcacac gtcctgaaca ggtgacattt
     3541 gatggcgaat gaagagacgc tgcgcgacta tctcaagtgg gtttcggcgg acctgcatca
     3601 gacgcagcaa cgtctgaagg acatcgaggc cgctgctcag gagcccatcg cgatcacagc
     3661 gatgagctgc cgcttccccg gcggtgtgag cggccccgaa gaactttggc gactcctttc
     3721 cgaaggccgg gacgccattt ctggcctccc cacggaccgt aactgggatc tcgaatccct
     3781 ctacgacccg gacgccgccg gaaagcaggg cacgacttcc acggccaacg gcggcttcct
     3841 ggacggcgtc gccgaattcg acgccgcatt cttcggcatc tccccgcgcg aagctctcgc
     3901 catggacccg cagcagcggc tcctcctgga aaccgcgtgg gaggccctgg aacgcgccgg
     3961 catcgacccg gcgacggtac gcggcagccg caccggcgta ttcgtcggca ccaacggaca
     4021 ggactacgcc ccgctcctct tcgaagccga ggaggacgtc gagggctatg tgagcaccgg
     4081 caatgccgcc gccgtcgagt ccggccgcat cgcctacacc ctcggcctcg aaggcccggc
     4141 cgtcaccgtg gacacggcct gctcctcgtc cctggtcgcc ctgcacctcg ccgtgcaggc
     4201 gctgcgccag ggcgagtgcg gcatggccct cgtcgcgggc gtcaccgtca tctccacccc
     4261 tggtgtcttc accgagttca gccgccagca gggcctcgcc gccgacggcc gctgcaaggc
     4321 cttcgccgac gcggccgacg gcaccggctg gggcgagggc gtcggcatgc tcctcgtcga
     4381 gcgcctctcc gacgcccgcc gcgcgggccg ccaggtcctc gccgtcgtcc gcggttccgc
     4441 cgtgaaccag gacggcgcca gcaacggcct gaccgccccg aacggcccct cccagcagcg
     4501 cgtcatcatg caggccctcg ccaacgcccg cctcaccgcg gcggacatcg acgcggtcga
     4561 ggcccacggc accggcacca cgctcggcga cccgatcgag gcgcaggccc tcctcgccac
     4621 ctacggccag gagcgcccgg acagcggcga acccctgtgg ctgggctcca tcaagtccaa
     4681 catcgggcac acccaggccg ccgccggtgt cgcgggcatc atcaagatgg tcatggcgat
     4741 gcgggcgggt gtcctcccgc agaccctcca cgtcgacgag cccacgtccc aggtgaactg
     4801 gtccgccggt gcggtcgccg tcctgaccga ggcccgcgca tgggccgagc gggagggcgg
     4861 cgcgccgcgt cgcgcgggcg tctcgtcctt cggcgtgagc ggtacgaacg cgcacgtcat
     4921 cgtggagcag gcaccggggg aggagacggg tgctggcgaa gccgacgctg acgccgcgcc
     4981 gtcctcttcg accgtgctgc cgggtgtcgt gccgctggcc ctctcgggca ggaccgaggg
     5041 cgcggtgcgg gcgcaggccg agcgggtgcg ggagctgctc cgtgcccagg acgggcttga
     5101 actgggcgat gtggcctggt cgttggctgt cggccgggcg cggttcgacc agcggggcgt
     5161 tgtcctcggg cgggaccggg acgaactgct cgccgggctt gaggcgctgg ctcaggacgg
     5221 cccgggcgcg cagaacgccg tcagcgggcg ggcgctgggc ggtgcggtac gcccggtgtt
     5281 cgtgttcccg gggcaggggt cgcagtgggc ggggatggcg cgtgagctgc tcgactccag
     5341 tccggtgttc gccgatcgga tgcgggagtg cggtgacgcg ctttcggagt tcgtcgagtg
     5401 gaatctgctc gatgagctga gcggggacac cttcgaccag gtggacgtgg tccagccggt
     5461 cctgttcgcg gtgatggtgt cgctggccgc gacctggcag gcggcgggtg ttcagcctgc
     5521 tgctgtggtc gggcacagtc agggtgagat cgcggccgcg tgtgtcgcgg gtgcgttgtc
     5581 gctgcgggac gcggcccgcg tggtggcgct gcggagcctg gcgatccggg agctgtcggg
     5641 caagggcggg atggtgtccg tgccgctgcc cgaggccgag gtgcgtgagc tgatcgctgc
     5701 ctgggacggc cggatcgagc ttgccgccgt gaacggtccg gcccaggtcg tggtctcggg
     5761 tgagcccgag gcgctggaag aactggtcgc ccagtgtgtc gcccaggaca tcc
//
