LOCUS       NZ_BMVR01000047         6084 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces flavofungini strain JCM 4753 sequence47, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVR01000047
VERSION     NZ_BMVR01000047.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces flavofungini
  ORGANISM  Streptomyces flavofungini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650815.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:46:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,873
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,638
            CDSs (with protein)               :: 7,638
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 43 of 161
            Pseudo Genes (incomplete)         :: 137 of 161
            Pseudo Genes (internal stop)      :: 10 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6084
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVR01000047.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..6064)
                     /locus_tag="IE274_RS38970"
     CDS             complement(<1..6064)
                     /NRPS_PKS="Domain: Epimerization (1-290). E-value:
                     2.8e-126. Score: 414.1. Matches aSDomain:
                     nrpspksdomains_IE274_RS38970_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (303-467). E-value: 1.2e-36.
                     Score: 117.9. Matches aSDomain:
                     nrpspksdomains_IE274_RS38970_TIGR01720.1"
                     /NRPS_PKS="Domain: Cglyc (474-767). E-value: 5.9e-108.
                     Score: 352.5. Matches aSDomain:
                     nrpspksdomains_IE274_RS38970_Cglyc.1"
                     /NRPS_PKS="Domain: AMP-binding (930-1324). E-value: 3e-104.
                     Score: 340.8. Matches aSDomain:
                     nrpspksdomains_IE274_RS38970_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (1430-1498). E-value: 8.1e-28.
                     Score: 88.4. Matches aSDomain:
                     nrpspksdomains_IE274_RS38970_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1513-1815). E-value:
                     8.2e-110. Score: 358.4. Matches aSDomain:
                     nrpspksdomains_IE274_RS38970_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE274_RS38970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_190120691.1"
                     /sec_met_domain="AMP-binding (E-value: 4e-112, bitscore:
                     360.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4e-17, bitscore:
                     48.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.3e-70, bitscore:
                     222.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTVVDLPQGIDETGLVATLGAVVDRHDMLRSRLLSGTPSGLEVA
                     EPGTVDVGALLHRVACDGRWDDANWPEQAAAELNEAADRLDAAAGTMAQFVWFDAGAGH
                     AGRLMVVLHHLVIDGVSWRILLPDLAAAWEQVRAGKTPVLAEVGTSVRRWSHALAEEAS
                     AAGRVAELPLWRSQVEGPDPVLGSRPLDPATDVMSTVRYVHMELPVATTTSLLTTLPAA
                     FHGGVNDGLLTALALAVATWRKTRGIDESSLLVRLEGHGREETAAPGADLSRTVGWFTS
                     MFPVRLDVAGVDLEDALDGGTAAGTAVKAVKEQLHAIPDKGIGYGLLRYLNPDTAEVLR
                     RHDTGQISFNYLGRYSATTDMPEHLRGLGFTQAPGTAGLVAEPDADMPALSALTVSAHV
                     TDTEQGPRFGARLDFPEGVLTRDEVQELADLWVNALDGLARHAAEPGAGGLTPSDVSLV
                     SVRQSEIEAWERQYPGVADVWPLTPLQAGMLFHSQLDESAYDAYQIQFVYHLSGAVDPA
                     RMRAAGQALLDRYANLRTAFVADAAGDFVQVVPERVDLPWRHLDLSGADTAEIDRFLAD
                     DRDVQFDASAPPLLRMSLLTLGPDRSELVFTAHHVLFDGWSIPLIMRDLLRLYGSGGDG
                     QALARVRGYRDFLEWLSEQDREAAAAAFAHELQGLDEPTLLVAGKSESHSDDVGNTEVP
                     LTPELARLLSACAAGLGVTLNTLVQGAWAVLLGQLTGRKDVVFGATVSGRPPTLSGVDE
                     MVGMFINTLPVRVRYASGDSLAEVLRDLQDRQAALLDHHHHPLSDVHRVAGVDTLFDTV
                     VVFESYPVDHVGLSEANAAAGVAITGLTPFTGTHYPLTVTADADPNLRLSLEYHRDVLD
                     EDAVQVIADRLHRLLRLLVADPDATIGSLDLLTQAERHTVLSGWNDTARPVAEATVPEL
                     FEAQVSRTPDRAAVRHGDTVLSYAELDARANRLAHLLISRGVRPEDLVALSVPRDAEMM
                     VAVLAVLKSGAAYLPIDPAYPADRVAFMVEDGAPALILTTSQVRATVGNRILLDSPEVR
                     AELRTMPDTAPGDSERNRPLLPSHAAYVIYTSGSTGRPKGVVIPHQNVVDFATWAVADL
                     GHDALSEVLAATSLNFDVSVFEMFGPLLSGGCIEVVQDVLALLGQDWRGSLISAVPSAL
                     SHMISQGGGTRLAADLVVLAGEGLPAHTLNAIRDAVPGARLANIYGPTEATVYAAAWYT
                     DEPAHTAPPIGRPLHNTRTYVLDAHVRPVPPGVAGELYLAGTGLARGYLGRPGLSAERF
                     VPDPFGPPGARMYRTGDVVRWTETGEIQYLGRVDNQVKVRGFRIELGEIEAVLSRHEDV
                     AQAHVLVREDRPGDQRITAYVVPERGVVPETATLRAHIAQALPEYMVPTAFVLLDKLPL
                     LPSGKLDRAALPRPEFTGAAYRAPRTASEEALCRLYAEVLGVERVGIDDDFFALGGHSL
                     LATRLVNRIRATLGAEVRVRLVFDAPTVVELSGRLSGDTTDRPLLRRTGTRPAQVPLSF
                     AQRRLWFVDRFEGPSATYNIPFLLRLTGALDLPALRAAVRDVVGRHESLRTLIAEDETG
                     HPFQQIVPTDELALDIPLTDVAPEAADEAVRRSVTQPFALAEEIPLRASVLRCGPQEHL
                     LLLVIHHIAGDGESMAPLARDLAAAYAARRDGVAPPWRELEVQYTDYALWQRDLLGDEN
                     DPASTLAAQGGYWREELAGVPQPLQLPTDRPRPPTASHRGDLVEFGLDPRVMAAVEELA
                     RSRGATVSMVLQSALAVLLGRLGGGEDITIGSPIANRADDKLSELVGFFVNTWVLRADL
                     SGNPSFEQVLDRVRAKALGAYDNQDAPFERLVELLNPERSTAYHPLFQVMFAWQNIARE
                     DFELRDLRVQLEPAFTSTAKFDLFFNMGDIPDAGVIGYLEYATDLFDRGTVENLAARFV
                     HLVEQLVTDPGLLVGSVDVLVADEVVALAGFNETAVVGPELTVPELFGRQVELSPDAVA
                     VVCGGVELTFAELDVRVRGLAGELVRRGV"
     source          1..6084
                     /culture_collection="JCM:4753"
                     /db_xref="taxon:68200"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces flavofungini"
                     /strain="JCM 4753"
                     /submitter_seqid="sequence47"
                     /type_material="type strain of Streptomyces flavofungini"
     region          1..6084
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..6084
                     /SMILES="NC(C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6084
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:6064](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..6064)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(461..580)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0021"
                     /evalue="3.30E-23"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1868"
                     /protein_start="1828"
                     /score="74.2"
                     /tool="antismash"
                     /translation="RAKALGAYDNQDAPFERLVELLNPERSTAYHPLFQVMFAW"
     aSDomain        complement(620..1525)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38970_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="8.20E-110"
                     /label="IE274_RS38970_Condensation_LCL.1"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1815"
                     /protein_start="1513"
                     /score="358.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAGDG"
                     /tool="antismash"
                     /translation="AQVPLSFAQRRLWFVDRFEGPSATYNIPFLLRLTGALDLPALRAA
                     VRDVVGRHESLRTLIAEDETGHPFQQIVPTDELALDIPLTDVAPEAADEAVRRSVTQPF
                     ALAEEIPLRASVLRCGPQEHLLLLVIHHIAGDGESMAPLARDLAAAYAARRDGVAPPWR
                     ELEVQYTDYALWQRDLLGDENDPASTLAAQGGYWREELAGVPQPLQLPTDRPRPPTASH
                     RGDLVEFGLDPRVMAAVEELARSRGATVSMVLQSALAVLLGRLGGGEDITIGSPIANRA
                     DDKLSELVGFFVNTWVLRADL"
     CDS_motif       complement(629..718)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0020"
                     /evalue="7.70E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1812"
                     /protein_start="1782"
                     /score="40.8"
                     /tool="antismash"
                     /translation="DITIGSPIANRADDKLSELVGFFVNTWVLR"
     CDS_motif       complement(986..1024)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0019"
                     /evalue="3.50E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1693"
                     /protein_start="1680"
                     /score="16.3"
                     /tool="antismash"
                     /translation="QYTDYALWQRDLL"
     CDS_motif       complement(1073..1138)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0018"
                     /evalue="2.20E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1664"
                     /protein_start="1642"
                     /score="26.6"
                     /tool="antismash"
                     /translation="IHHIAGDGESMAPLARDLAAAY"
     CDS_motif       complement(1349..1459)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0017"
                     /evalue="4.70E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1572"
                     /protein_start="1535"
                     /score="54.4"
                     /tool="antismash"
                     /translation="ATYNIPFLLRLTGALDLPALRAAVRDVVGRHESLRTL"
     CDS_motif       complement(1502..1555)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0016"
                     /evalue="1.80E+01"
                     /label="C5_starter_265-294"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1521"
                     /protein_start="1503"
                     /score="-1.8"
                     /tool="antismash"
                     /translation="PLLRRTGTRPAQVPLSFA"
     aSDomain        complement(1571..1774)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38970_PCP.1"
                     /evalue="8.10E-28"
                     /label="IE274_RS38970_PCP.1"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1498"
                     /protein_start="1430"
                     /score="88.4"
                     /tool="antismash"
                     /translation="EEALCRLYAEVLGVERVGIDDDFFALGGHSLLATRLVNRIRATLG
                     AEVRVRLVFDAPTVVELSGRLSG"
     aSModule        complement(1571..4642)
                     /complete
                     /domains="nrpspksdomains_IE274_RS38970_Cglyc.1"
                     /domains="nrpspksdomains_IE274_RS38970_AMP-binding.1"
                     /domains="nrpspksdomains_IE274_RS38970_PCP.1"
                     /locus_tags="IE274_RS38970"
                     /monomer_pairings="Ala -> Ala"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2063..2128)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0015"
                     /evalue="3.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1334"
                     /protein_start="1312"
                     /score="38.2"
                     /tool="antismash"
                     /translation="YLGRVDNQVKVRGFRIELGEIE"
     aSDomain        complement(2093..3274)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38970_AMP-binding.1"
                     /evalue="3.00E-104"
                     /label="IE274_RS38970_AMP-binding.1"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1324"
                     /protein_start="930"
                     /score="340.8"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="FEAQVSRTPDRAAVRHGDTVLSYAELDARANRLAHLLISRGVRPE
                     DLVALSVPRDAEMMVAVLAVLKSGAAYLPIDPAYPADRVAFMVEDGAPALILTTSQVRA
                     TVGNRILLDSPEVRAELRTMPDTAPGDSERNRPLLPSHAAYVIYTSGSTGRPKGVVIPH
                     QNVVDFATWAVADLGHDALSEVLAATSLNFDVSVFEMFGPLLSGGCIEVVQDVLALLGQ
                     DWRGSLISAVPSALSHMISQGGGTRLAADLVVLAGEGLPAHTLNAIRDAVPGARLANIY
                     GPTEATVYAAAWYTDEPAHTAPPIGRPLHNTRTYVLDAHVRPVPPGVAGELYLAGTGLA
                     RGYLGRPGLSAERFVPDPFGPPGARMYRTGDVVRWTETGEIQYLGRVDNQVKVR"
     CDS_motif       complement(2213..2302)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0014"
                     /evalue="2.00E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1284"
                     /protein_start="1254"
                     /score="48.9"
                     /tool="antismash"
                     /translation="PPGVAGELYLAGTGLARGYLGRPGLSAERF"
     CDS_motif       complement(2753..2797)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0013"
                     /evalue="4.90E+00"
                     /label="NRPS-E2"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1104"
                     /protein_start="1089"
                     /score="0.3"
                     /tool="antismash"
                     /translation="VIPHQNVVDFATWAV"
     CDS_motif       complement(2786..2845)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0012"
                     /evalue="1.30E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1093"
                     /protein_start="1073"
                     /score="33.1"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVVIPH"
     CDS_motif       complement(3047..3088)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0011"
                     /evalue="4.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="IE274_RS38970"
                     /protein_end="1006"
                     /protein_start="992"
                     /score="16.7"
                     /tool="antismash"
                     /translation="LAVLKSGAAYLPID"
     CDS_motif       complement(3608..3721)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0010"
                     /evalue="1.90E-16"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="IE274_RS38970"
                     /protein_end="819"
                     /protein_start="781"
                     /score="52.4"
                     /tool="antismash"
                     /translation="QDRQAALLDHHHHPLSDVHRVAGVDTLFDTVVVFESYP"
     aSDomain        complement(3764..4642)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38970_Cglyc.1"
                     /domain_subtypes="Cglyc"
                     /evalue="5.90E-108"
                     /label="IE274_RS38970_Cglyc.1"
                     /locus_tag="IE274_RS38970"
                     /protein_end="767"
                     /protein_start="474"
                     /score="352.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHVLFDG"
                     /tool="antismash"
                     /translation="VADVWPLTPLQAGMLFHSQLDESAYDAYQIQFVYHLSGAVDPARM
                     RAAGQALLDRYANLRTAFVADAAGDFVQVVPERVDLPWRHLDLSGADTAEIDRFLADDR
                     DVQFDASAPPLLRMSLLTLGPDRSELVFTAHHVLFDGWSIPLIMRDLLRLYGSGGDGQA
                     LARVRGYRDFLEWLSEQDREAAAAAFAHELQGLDEPTLLVAGKSESHSDDVGNTEVPLT
                     PELARLLSACAAGLGVTLNTLVQGAWAVLLGQLTGRKDVVFGATVSGRPPTLSGVDEMV
                     GMFINTLPVRVR"
     CDS_motif       complement(3770..3865)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0009"
                     /evalue="1.40E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="IE274_RS38970"
                     /protein_end="765"
                     /protein_start="733"
                     /score="62.5"
                     /tool="antismash"
                     /translation="DVVFGATVSGRPPTLSGVDEMVGMFINTLPVR"
     CDS_motif       complement(4109..4135)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0008"
                     /evalue="1.50E+01"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE274_RS38970"
                     /protein_end="652"
                     /protein_start="643"
                     /score="-1.0"
                     /tool="antismash"
                     /translation="YRDFLEWLS"
     CDS_motif       complement(4178..4240)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0007"
                     /evalue="1.80E-09"
                     /label="C3_DCL_135-156"
                     /locus_tag="IE274_RS38970"
                     /protein_end="629"
                     /protein_start="608"
                     /score="30.1"
                     /tool="antismash"
                     /translation="HHVLFDGWSIPLIMRDLLRLY"
     CDS_motif       complement(4451..4567)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0006"
                     /evalue="9.40E-16"
                     /label="C2_DCL_024-062"
                     /locus_tag="IE274_RS38970"
                     /protein_end="538"
                     /protein_start="499"
                     /score="50.1"
                     /tool="antismash"
                     /translation="DAYQIQFVYHLSGAVDPARMRAAGQALLDRYANLRTAFV"
     CDS_motif       complement(4592..4627)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0005"
                     /evalue="5.10E-04"
                     /label="C1_dual_004-017"
                     /locus_tag="IE274_RS38970"
                     /protein_end="491"
                     /protein_start="479"
                     /score="13.0"
                     /tool="antismash"
                     /translation="PLTPLQAGMLFH"
     aSDomain        complement(4664..5155)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38970_TIGR01720.1"
                     /evalue="1.20E-36"
                     /label="IE274_RS38970_TIGR01720.1"
                     /locus_tag="IE274_RS38970"
                     /protein_end="467"
                     /protein_start="303"
                     /score="117.9"
                     /tool="antismash"
                     /translation="AGTAVKAVKEQLHAIPDKGIGYGLLRYLNPDTAEVLRRHDTGQIS
                     FNYLGRYSATTDMPEHLRGLGFTQAPGTAGLVAEPDADMPALSALTVSAHVTDTEQGPR
                     FGARLDFPEGVLTRDEVQELADLWVNALDGLARHAAEPGAGGLTPSDVSLVSVRQSEIE
                     A"
     aSModule        complement(4664..6061)
                     /domains="nrpspksdomains_IE274_RS38970_Epimerization.1"
                     /domains="nrpspksdomains_IE274_RS38970_TIGR01720.1"
                     /incomplete
                     /locus_tags="IE274_RS38970"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(5072..5134)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0004"
                     /evalue="5.30E-12"
                     /label="NRPS-E6"
                     /locus_tag="IE274_RS38970"
                     /protein_end="331"
                     /protein_start="310"
                     /score="37.7"
                     /tool="antismash"
                     /translation="VKEQLHAIPDKGIGYGLLRYL"
     aSDomain        complement(5195..6061)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38970_Epimerization.1"
                     /evalue="2.80E-126"
                     /label="IE274_RS38970_Epimerization.1"
                     /locus_tag="IE274_RS38970"
                     /protein_end="290"
                     /protein_start="1"
                     /score="414.1"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVIDG"
                     /tool="antismash"
                     /translation="STVVDLPQGIDETGLVATLGAVVDRHDMLRSRLLSGTPSGLEVAE
                     PGTVDVGALLHRVACDGRWDDANWPEQAAAELNEAADRLDAAAGTMAQFVWFDAGAGHA
                     GRLMVVLHHLVIDGVSWRILLPDLAAAWEQVRAGKTPVLAEVGTSVRRWSHALAEEASA
                     AGRVAELPLWRSQVEGPDPVLGSRPLDPATDVMSTVRYVHMELPVATTTSLLTTLPAAF
                     HGGVNDGLLTALALAVATWRKTRGIDESSLLVRLEGHGREETAAPGADLSRTVGWFTSM
                     FPVRLDVA"
     CDS_motif       complement(5204..5254)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0003"
                     /evalue="2.10E-09"
                     /label="NRPS-E5"
                     /locus_tag="IE274_RS38970"
                     /protein_end="287"
                     /protein_start="270"
                     /score="29.2"
                     /tool="antismash"
                     /translation="DLSRTVGWFTSMFPVRL"
     CDS_motif       complement(5678..5743)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0002"
                     /evalue="8.60E-11"
                     /label="NRPS-E2"
                     /locus_tag="IE274_RS38970"
                     /protein_end="129"
                     /protein_start="107"
                     /score="34.0"
                     /tool="antismash"
                     /translation="LMVVLHHLVIDGVSWRILLPDL"
     CDS_motif       complement(5963..6061)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38970_0001"
                     /evalue="1.30E-04"
                     /label="C2_DCL_024-062"
                     /locus_tag="IE274_RS38970"
                     /protein_end="34"
                     /protein_start="1"
                     /score="14.3"
                     /tool="antismash"
                     /translation="STVVDLPQGIDETGLVATLGAVVDRHDMLRSRL"
ORIGIN
        1 ccacaccacg ccggaccaac tcacccgcca gcccacgcac ccgcacatca agctcggcga
       61 acgtcagctc cacaccacca cacaccaccg ccaccgcatc cggcgacaac tccacctgcc
      121 gcccgaacaa ctccggaacc gtcaactccg gccccaccac agccgtctca ttgaacccag
      181 ccagcgccac cacctcatcc gccaccaaca catccaccga acccaccaac aaccccgggt
      241 cggtgacgag ttgctcgacg aggtgcacga agcgggccgc gaggttctcc accgtgccgc
      301 ggtcgaacag atcggtcgcg tactccaggt atccgatcac cccggcgtcg gggatgtcgc
      361 ccatgttgaa gaacaggtcg aacttcgcgg tcgatgtgaa ggcgggttcg agctgaaccc
      421 gcaggtcgcg gagttcgaag tcctcgcgtg cgatgttctg ccaggcgaac atcacctgga
      481 acagcgggtg gtacgccgtg gagcgctccg gattcagcag ctccaccaga cgctcgaacg
      541 gggcgtcctg gttgtcgtag gcgcccagcg ccttggcccg cacccggtcg aggacctgct
      601 cgaacgacgg gttcccggac aggtcggcgc gcagcaccca ggtgttgacg aagaagccga
      661 ccagttcgga gagcttgtcg tcggcgcggt tggcgatcgg ggagccgatc gtgatgtcct
      721 cgccgccgcc gagccggccg agcagcaccg ccagtgccga ctggagcacc atggacaccg
      781 tcgcgccccg ggaccgggcc agctcctcga ccgcggccat cacccggggg tccaggccga
      841 actcgacgag gtcaccgcgg tggctcgccg tcgggggccg cggccggtcc gtcggcagtt
      901 gcaggggctg cggtacgccc gccagctcct cgcgccagta acctccctgc gcggccaggg
      961 tgctcgcggg atcgttctcg tcgccgagga ggtcccgctg ccacagggcg tagtccgtgt
     1021 actgcacctc aagctcccgc cacgggggtg ccactccgtc ccggcgtgcc gcgtaggcgg
     1081 cggccaggtc gcgggcgagc ggggccatcg actcgccgtc accggcgatg tggtggatca
     1141 ccagcagcag caggtgctcc tgggggccgc accgcagcac cgaggcccgc agcggaatct
     1201 cctcggccag ggcgaagggc tgggtcacgc tccggcgcac cgcctcgtcc gccgcctccg
     1261 gcgccacgtc ggtgagcgga atgtccagcg ccaactcgtc cgtcggcacg atctgctgga
     1321 acgggtgccc ggtctcgtcc tcggcgatca gcgtccgcag gctctcgtgc cgcccgacga
     1381 cgtcccggac cgccgcgcgc agtgcgggca ggtcgagggc gccggtcagc cgcagcagga
     1441 acgggatgtt gtacgtcgcc gacgggccct cgaaccggtc cacgaaccac agtcggcgct
     1501 gcgcgaagga cagcgggacc tgtgcgggcc gtgtcccggt gcgccgcagg agcgggcggt
     1561 ccgtcgtgtc gccggacagc cgcccgctca gctcgacgac ggtcggcgcg tcgaacacga
     1621 ggcggacgcg cacctcggcg cccagcgtcg cgcggatccg gttgacgagc cgggtggcga
     1681 gcagcgaatg cccgcccagc gcgaagaagt cgtcatcgat gccgacccgc tccacgccga
     1741 gcacttcggc gtacaggcgg cacagcgcct cttcactcgc ggtgcgcggc gcccggtagg
     1801 ccgcgccggt gaactccggc cggggcaggg cggcgcggtc cagcttcccg ctgggcagca
     1861 gcggcaactt gtcgagcagc acgaacgcgg tgggcaccat gtactcgggc agcgcctgcg
     1921 cgatgtgcgc gcgcagcgtc gcggtctcgg gtacgacgcc gcgctccggc acgacgtagg
     1981 cggtgatccg ctggtctccg ggccggtcct cgcggacgag gacgtgggcc tgcgccacgt
     2041 cctcgtggcg gctgaggacg gcctcgatct cgccgagctc gatccggaag ccgcggacct
     2101 tcacctggtt gtcgacgcgg ccgaggtact ggatctcgcc ggtctcggtc cagcggacga
     2161 cgtccccggt gcggtacatc cgggcgccgg gcgggccgaa ggggtcgggc acgaagcgct
     2221 ccgcgctcag cccgggacgg cccaggtagc cgcgggccag tccggtgccc gcgagataga
     2281 gctcacccgc cacccccggc ggcacgggcc ggacgtgcgc gtcgagcaca taggtccggg
     2341 tgttgtggag ggggcggccg atggggggcg cggtgtgcgc cggttcgtcg gtgtaccagg
     2401 cggcggcgta cacggtggcc tcggtgggcc cgtagatgtt ggcgaggcgg gcgccgggga
     2461 cggcgtcgcg gatggcgttg agggtgtgcg cgggcaggcc ttcaccggcc agcacgacga
     2521 ggtcggccgc cagccgggtc ccgccgccct ggctgatcat gtgcgagagg gccgacggca
     2581 ccgcgctgat caggctgccg cgccagtcct ggccgaggag cgccaggacg tcctggacga
     2641 cctcgatgca gccgccgctg agcagcgggc cgaacatctc gaagacggag acgtcgaagt
     2701 tgagggaggt ggcggccagg acctcggaga gcgcgtcgtg gcccaggtcg gccacggccc
     2761 aggtcgcgaa gtcgacgacg ttctggtgcg ggatgaccac acccttgggc cggccggtgg
     2821 agccggacgt gtagatcacg tacgcggcgt gcgagggcag cagcgggcgg ttccgctcgc
     2881 tgtccccggg cgcggtgtcg ggcatggtcc gcagctcggc ccgcacctcg ggcgagtcga
     2941 gcaggatccg gttgccgacg gtcgcgcgca cctgactggt ggtcagaatc agggccgggg
     3001 cgccgtcctc gaccatgaag gcgacacggt cggccgggta ggccgggtcg atgggcaggt
     3061 aggccgcccc cgacttgagc accgcgagca cggccaccat catctcggcg tcgcgcggca
     3121 ccgagagcgc gaccaggtcc tccggccgta caccgcggga gatcagcagg tgggcgagcc
     3181 ggttggcgcg ggcgtcaagc tcggcgtagc tgaggacggt gtccccgtgc cggacggccg
     3241 cccggtcggg ggtgcgggac acctgggcct cgaagagctc cggaacggtc gcctcggcga
     3301 ccgggcgggc ggtgtcgttc cacccggaca gcacggtgtg gcgctccgcc tgcgtcagca
     3361 ggtccagcga gccgatcgtg gcgtccggat cggccaccag caggcgcagc aggcggtgca
     3421 gccggtccgc gatcacctgc accgcgtcct cgtcgagcac gtcgcggtgg tactccaggg
     3481 acagccggag gttcgggtcg gcgtcggccg tgacggtcag cgggtagtgg gtgccggtga
     3541 acggggtgag cccggtgatc gccaccccgg cggcggcgtt ggcctcgctg agaccgacgt
     3601 ggtcgaccgg gtaggactcg aagacgacga ccgtgtcgaa cagggtgtcc accccggcga
     3661 cccggtggac gtcgctcaac gggtggtggt ggtggtcgag cagcgcggcc tgccggtcct
     3721 gcaggtccct cagcacctcg gcgagggagt ccccggaggc gtagcgcacg cgcaccggca
     3781 gggtgttgat gaacatcccc accatctcgt ccacgccgga gagcgtgggc ggccgtcccg
     3841 agaccgtggc gccgaagacg acgtccttcc gtccggtcag ctgaccgagc aggaccgccc
     3901 aggcgccctg taccagcgtg ttcagggtga ccccgaggcc ggcggcgcac gcggacagga
     3961 ggcgggcgag ttccggcgtc agcgggacct cggtgttgcc gacgtcgtcg ctgtgcgact
     4021 cggacttccc cgccaccagc agggtgggct cgtcgagccc ttggagctcg tgcgcgaacg
     4081 ccgcggcggc cgcctcgcgg tcctgctccg agagccactc caggaagtcg cggtacccgc
     4141 gtacgcgcgc cagcgcctgg ccgtcgccgc cggatccgta gagccgcagc aggtcccgca
     4201 tgatcagcgg tatggaccag ccgtcgaaca gcacgtggtg ggcggtgaag accagttccg
     4261 agcggtccgg accgagcgtc agcaaggaca tccgcagcag cggcggggcg ctcgcgtcga
     4321 actggacgtc gcggtcgtcg gccaggaacc ggtcgatctc cgccgtgtcc gcaccgctga
     4381 ggtcgaggtg acgccacggc aggtcgacgc gctccggcac gacctggacg aagtcaccgg
     4441 cggcgtccgc cacgaaggcg gtgcgcaggt tggcgtaccg gtcgagcagg gcctggcccg
     4501 ccgcgcgcat ccgcgccggg tcgaccgcgc ccgagaggtg gtagacgaac tggatctgat
     4561 aggcgtcgta ggcggactcg tccagctgcg agtggaagag catgcccgcc tgcagcgggg
     4621 tcagcggcca gacgtcggcc acccccgggt actgccgctc ccaggcctcg atctcgctct
     4681 gccggaccga caccagggag acgtccgagg gcgtcaggcc gcccgcgccg ggctcggccg
     4741 cgtgccgggc caggccgtcc agggcgttca cccagaggtc ggccagctcc tgcacctcgt
     4801 cccgggtgag gacgccctcg gggaagtcga ggcgggcgcc gaagcgcggc ccctgctcgg
     4861 tgtcggtgac gtgcgcgctc acggtcaggg ccgagagggc cggcatgtcg gcgtccggct
     4921 cggcgaccag gcccgcggtg cccggcgcct gggtgaagcc caggccgcgc aggtgttccg
     4981 gcatgtccgt cgtggccgag tagcggccca ggtagttgaa ggagatctgg ccggtgtcgt
     5041 gccggcggag gacctccgcg gtgtccgggt tgaggtagcg cagcaggccg tagccgatgc
     5101 ccttgtccgg gatggcgtgc agttgctcct tgaccgcctt gacggccgta ccggccgccg
     5161 tgccgccgtc gagcgcgtcc tccaggtcga ccccggcgac gtcgaggcgt acggggaaca
     5221 tgctggtgaa ccagcccacc gtgcgcgaca ggtccgcacc cggggccgcg gtctcctcgc
     5281 ggccgtggcc ctccaggcgc accagcagcg aggactcgtc gatgccgcgc gtcttgcgcc
     5341 aggtggcgac ggccagggcg agggcggtga gcaggccgtc gttgacgccg ccgtggaagg
     5401 ccgcgggcag ggtggtcagg agcgacgtgg tggtggcgac cggcagttcc atgtggacgt
     5461 agcggaccgt ggacatcacg tcggtggcgg ggtcgagcgg gcgggagccg agcaccgggt
     5521 ccggcccttc cacctgggag cgccacagcg gcagttccgc cacgcgcccc gcagcggacg
     5581 cctcctcggc cagggcgtgc gaccagcgcc gcacggaagt ccccacctcc gcgaggaccg
     5641 gagtcttgcc cgcccgcacc tgctcccagg ccgcagcgag atcaggcagc aggatgcgcc
     5701 aggagacacc gtcgatcacc aggtggtgca gcaccaccat cagtcgaccc gcgtgcccgg
     5761 cgcccgcgtc gaaccacacg aactgcgcca tggtcccggc cgcggcgtcc agccggtccg
     5821 cggcctcgtt cagctccgcg gcagcctgct ccggccagtt cgcgtcgtcc cagcggccgt
     5881 cgcacgcgac gcggtgcagg agggcgccga cgtcgaccgt gccgggctcc gcgacctcca
     5941 ggcccgacgg ggtgccggac agcagtcggg agcggagcat gtcgtggcgg tcgacgaccg
     6001 cacccagcgt ggcgaccagc ccggtctcat cgatgccctg cggcaggtcc accaccgtcg
     6061 acatcgtgaa ccggtcgata cccc
//
