LOCUS       NZ_BMVR01000043         9753 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces flavofungini strain JCM 4753 sequence43, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVR01000043
VERSION     NZ_BMVR01000043.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces flavofungini
  ORGANISM  Streptomyces flavofungini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650815.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:46:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,873
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,638
            CDSs (with protein)               :: 7,638
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 43 of 161
            Pseudo Genes (incomplete)         :: 137 of 161
            Pseudo Genes (internal stop)      :: 10 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9753
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVR01000043.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..9749)
                     /NRPS_PKS="Domain: PCP (58-126). E-value: 1e-26. Score:
                     84.9. Matches aSDomain: nrpspksdomains_IE274_RS38910_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (141-443). E-value:
                     1.4e-111. Score: 364.2. Matches aSDomain:
                     nrpspksdomains_IE274_RS38910_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (610-1009). E-value:
                     1.3e-105. Score: 345.3. Matches aSDomain:
                     nrpspksdomains_IE274_RS38910_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (1117-1186). E-value: 9.2e-30.
                     Score: 94.7. Matches aSDomain:
                     nrpspksdomains_IE274_RS38910_PCP.2"
                     /NRPS_PKS="Domain: Condensation_LCL (1200-1502). E-value:
                     7.7e-110. Score: 358.5. Matches aSDomain:
                     nrpspksdomains_IE274_RS38910_Condensation_LCL.2"
                     /NRPS_PKS="Domain: AMP-binding (1668-2062). E-value: 3e-93.
                     Score: 304.6. Matches aSDomain:
                     nrpspksdomains_IE274_RS38910_AMP-binding.2"
                     /NRPS_PKS="Domain: PCP (2171-2238). E-value: 1.2e-23.
                     Score: 75.0. Matches aSDomain:
                     nrpspksdomains_IE274_RS38910_PCP.3"
                     /NRPS_PKS="Domain: Epimerization (2255-2571). E-value:
                     2.2e-130. Score: 427.7. Matches aSDomain:
                     nrpspksdomains_IE274_RS38910_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (2584-2746). E-value: 5.9e-37.
                     Score: 118.8. Matches aSDomain:
                     nrpspksdomains_IE274_RS38910_TIGR01720.1"
                     /NRPS_PKS="Domain: Condensation_DCL (2756-3053). E-value:
                     2.1e-101. Score: 331.0. Matches aSDomain:
                     nrpspksdomains_IE274_RS38910_Condensation_DCL.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE274_RS38910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_190120680.1"
                     /sec_met_domain="Condensation (E-value: 1.5e-72, bitscore:
                     230.3, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.5e-118, bitscore:
                     380.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.4e-20, bitscore:
                     58.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAELRSYLADRLPAHLVPASMVAIPELPLTANGKLDRKALPAPDF
                     AAVATGRTPRTPQEELLCGLFAEVLGLDRVGIDDSFFDLGGHSLLATRLVSRVRAVLGV
                     ELPIRMVFTAPTVVDLVEHLGQGERVRPPLTRVTPRPERVPLSFAQRRLWFIDRFEGLS
                     ATYNAPFPLRLKGELDADALRAAVRDVVARHESLRTVFAEDEDGVPFQRVLPAAEAHVD
                     VPVLEVEPAGLERAIVDFMSHRFDLAAEIPVKAVLLRLGAADHMLVWLVHHIACDGASM
                     APMARDLTTAYTARVADGLPQWTELPAQYVDYTLWQRDLLGDGNDPDSLLSAQVAYWQE
                     ELADIPQPLQLPTDRPRPAEASHRGDSLEFTLDAELMTTVDQLARTHGATSSMVLEAAL
                     AVLLHSLGGGDDITIGSPIANRTDEGLADLVGFFVNTWVLRTRLAGNPTFTDVLDDVRT
                     KSLAAYDHQDVPFERLVEALNPERSTAYAPLFQVMFAWQNNSRQDFALPGLDVALEWPH
                     SQSSKFDLTFNLMAETGPGGAVGSVEYATDLFDATTVRKLADRYVRLLRQLTADPRRRI
                     GSVDLLAADERELVLRGFNDTAKPAVRHSMAEVIAEHVTAAPDRLAVVSAEQELTYREL
                     DARANRLARVLIAHGVRRDSVVGLALPRSADQVVAVIAILKAGGCYLPLDPEYPAERLA
                     YMLRDSAPPLVVTDTENASSLPDNVCPALVLDEPDTAAAVARESAEPLTVAGHPDQLAY
                     VMYTSGSTGRPKGVGVTQRGVVRLSAESGYREGAHERVLTQCAQAFDCNTYEQWFPLLK
                     GGTLVIAPPGHVDTATLARLVTEHRLTAVMSSSGIFRVIAEELPEAFAGLRDTLTGGEV
                     VPRSAYENILAACPGTRVINGYGPTEATMACTFHGVTAHEGIGVLGEIGRPMDNTRIYL
                     LDSALRPVLPGVPGELYIAGDRLARGYLGRFDLTAERFVACPFGDTPGERMYRTGDIAT
                     WTPEGSLVYHGRADTQVKIRGFRIEPGEVEAALTRHGAVAQAVVITRDSAEFGKQLIAY
                     TVPSVTESGTDLADLRSFAAGQLPDHMVPSAFVSLDSIPLSPSGKVDHRALPEPEFTSA
                     KPYRRPRTPAEETLAGVFADVLGADRVGIDDSFFDLGGHSLLATRLVSRIRAALGVELP
                     IRTVFAAPTVAALAERLDQGERVRPPLTRVEPRPERAPLSYAQRRLWFIDRFEGPSATY
                     NAPFPLRLSGELDAVALAAAMRDVVARHESLRTVFAEDEDGVPCQRVLPAHEAQLDVAV
                     VDVEPGGVAAALAEFAAHRFDLAQEIPVKAALLRLGAADHVLVWLVHHIACDGASLAPM
                     ARDLNTAYTARLADGLPQWTELPAQYVDYTLWQRDLLGDENDPNSLLSTQVAYWREELA
                     DIPQPLQLPTDRPRPRRASHDGAAVEFSVDNRIMSAVADLARTHGATESMVLEAALAVL
                     LHGLGGGDDITIGSPIANRTDENLTDLVGFFVNTWVLRTRLAGNPTFADVLDQVRAKSL
                     AAYDHQDVPFERLVEELNPERSTAYAPLFQVMFAWQNVDLDGFELPGLGVEFERAYSDS
                     AKFDLTFHMVDLPELGGVVGSLEYATDLFDDPTVRKLADRYVRLLDRLTADPRQHVRGV
                     ELVEPDERELVLSAFNDTAAATPELTAAELFAQRAAATPEATALVCEDQKWSYAELAAR
                     AGRLAAELAGRGVGPETVVGLALPRSADLVVAMLGIWRAGGAYLPIDPKYPSSRLDFIL
                     ADARPALVLTDASAVCVLTGADVPHLLVDDLDLDPDAHDTPARDREPAVVRPLNAAYVM
                     YTSGSTGTPKGVTVTHRDVVNGVLRLAEAVDIHAGTRMLAGTSVNFDVSVFEIVTTLAA
                     GGTVELVRDVLVIGERGGWDGGVISTVPSVFAELVDQIGDRIEADAVVFAGEALPAALV
                     RSAHEALPGVRVVNAYGQTESFYATTFTADTERTGGTSAPIGTPLGNMRTYVLGSGLAP
                     VPVGVVGELYVAGNVARGYHGRASLTAERFLPDPFGPPGARMYRTGDRARWTSQGHLEY
                     VSRDDAQVKVRGFRIEPGEIEAALTAHAGVAQAVVGTRDVRGSTQLVGYVVPAADAGIG
                     AGVSAKALRAFVAERLPEFMVPSVFVTLDRLPLAPNGKLDHSALPEPECTGGEYHAPRT
                     AAEEILAAVYAEVLGLDRVGVDDDFFAIGGDSIRSIQVVSRARAQGVEVTPRQIFECRT
                     VAELADVAAAGRQATVLAELDGGGVGWMPLLPIGRYLLELADQGGGFGRFAMSMEVELP
                     GGIDERGLAATLGAVLDHHDILRSRLVVTADGDAGLHVTAPGSVDPAALIHRVACDGRW
                     DEAAWRDRAAAELDRATGRLDPAAGVMAQFVWCDPGPGTPGRLLLVLHHFLVDGVSWRV
                     LLPDLAAAWTDIRDGRTPRLEPVATSVRRWSHALAEEATSPRRTAELDHWRAVVSGPDP
                     DLGSRAFDPAVDVSGTVAHVSVELPVAATEALLTTVPAAFRTGVNDGLLTAFGLALAKW
                     RRARGVDEPSALIRLEGHGREEEAAPGADLSRTVGWLTSMFPVRVDVGGVDLEDAFSGG
                     RAAGQVLKAVKEQLHAVPGNGIGYGLLRYLNPETADILKPATGGQISFNYLGRFAEDTP
                     EELRGLGWTPADDNGLRGAELDADMPAMATIEVTSLVSDTAQGPRLTAQLSFPSGLLAH
                     DDARELIELWRSALENLALHAAAPGSGGLTPSDVPLVRVSQRDLDRWHDRYPGAVADVW
                     PVTATQSGILFHGMLAESSFDAYHTQLAFSLSGNVDPERMRTAGQALLDRHAALRTAFV
                     TSAAGEQVQLVLEDVELPWQAVDLTDLDEAERDAAFERLLAADQAVHFEPDRPPLLRLS
                     LIRRGPDRTELVFTANHALYDGWSIPHLLRELLRLYGSGGAPSALPRVRPYRDFLVWLS
                     QQDEEAAARAWARELDGVTEPTLLATRAGATTESEGLGQIEVPLPDPAARELSRRASEL
                     GVTLNTLLQGTWGLLLGYLTDRRDVVLGTTVSGRPPQVAGVDEMVGLFINTLPVRVTWA
                     PDATLAQTLTSLQERQTPLLDHHHHSLSRIHETMGLPTLFDTMVVLESYPVDEAGLEEA
                     HAVAGAALTGVRATTGTHYPLAVVAAAEPHLRAELQYQENLFPKAAAERIAARFGQLLE
                     RVAADPHLPVARLDLLDGTERDQVLERFNDTGRERAGCGIAEVFQEQATADPERVALVS
                     GEEELSYADLDERSNRLAR"
     source          1..9753
                     /culture_collection="JCM:4753"
                     /db_xref="taxon:68200"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces flavofungini"
                     /strain="JCM 4753"
                     /submitter_seqid="sequence43"
                     /type_material="type strain of Streptomyces flavofungini"
     gene            complement(<1..>9753)
                     /locus_tag="IE274_RS38910"
     region          1..9753
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9753
                     /SMILES="NC(C(C)C)C(=O)NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9753
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:9749](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..9749)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(436..549)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0034"
                     /evalue="2.40E-09"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="IE274_RS38910"
                     /protein_end="3104"
                     /protein_start="3066"
                     /score="29.6"
                     /tool="antismash"
                     /translation="QERQTPLLDHHHHSLSRIHETMGLPTLFDTMVVLESYP"
     aSDomain        complement(589..1479)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="2.10E-101"
                     /label="IE274_RS38910_Condensation_DCL.1"
                     /locus_tag="IE274_RS38910"
                     /protein_end="3053"
                     /protein_start="2756"
                     /score="331.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: NHALYDG"
                     /tool="antismash"
                     /translation="DVWPVTATQSGILFHGMLAESSFDAYHTQLAFSLSGNVDPERMRT
                     AGQALLDRHAALRTAFVTSAAGEQVQLVLEDVELPWQAVDLTDLDEAERDAAFERLLAA
                     DQAVHFEPDRPPLLRLSLIRRGPDRTELVFTANHALYDGWSIPHLLRELLRLYGSGGAP
                     SALPRVRPYRDFLVWLSQQDEEAAARAWARELDGVTEPTLLATRAGATTESEGLGQIEV
                     PLPDPAARELSRRASELGVTLNTLLQGTWGLLLGYLTDRRDVVLGTTVSGRPPQVAGVD
                     EMVGLFINTLPVRVTW"
     CDS_motif       complement(598..693)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0033"
                     /evalue="7.70E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="IE274_RS38910"
                     /protein_end="3050"
                     /protein_start="3018"
                     /score="60.1"
                     /tool="antismash"
                     /translation="DVVLGTTVSGRPPQVAGVDEMVGLFINTLPVR"
     CDS_motif       complement(937..966)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0032"
                     /evalue="1.10E+01"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2937"
                     /protein_start="2927"
                     /score="-0.6"
                     /tool="antismash"
                     /translation="YRDFLVWLSQ"
     CDS_motif       complement(1009..1068)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0031"
                     /evalue="3.20E-06"
                     /label="C3_DCL_135-156"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2913"
                     /protein_start="2893"
                     /score="19.7"
                     /tool="antismash"
                     /translation="HALYDGWSIPHLLRELLRLY"
     CDS_motif       complement(1294..1410)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0030"
                     /evalue="8.10E-17"
                     /label="C2_DCL_024-062"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2818"
                     /protein_start="2779"
                     /score="53.5"
                     /tool="antismash"
                     /translation="DAYHTQLAFSLSGNVDPERMRTAGQALLDRHAALRTAFV"
     aSDomain        complement(1510..1995)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_TIGR01720.1"
                     /evalue="5.90E-37"
                     /label="IE274_RS38910_TIGR01720.1"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2746"
                     /protein_start="2584"
                     /score="118.8"
                     /tool="antismash"
                     /translation="AGQVLKAVKEQLHAVPGNGIGYGLLRYLNPETADILKPATGGQIS
                     FNYLGRFAEDTPEELRGLGWTPADDNGLRGAELDADMPAMATIEVTSLVSDTAQGPRLT
                     AQLSFPSGLLAHDDARELIELWRSALENLALHAAAPGSGGLTPSDVPLVRVSQRDLDR"
     aSModule        complement(1510..6147)
                     /complete
                     /domains="nrpspksdomains_IE274_RS38910_Condensation_LCL.2"
                     /domains="nrpspksdomains_IE274_RS38910_AMP-binding.2"
                     /domains="nrpspksdomains_IE274_RS38910_PCP.3"
                     /domains="nrpspksdomains_IE274_RS38910_Epimerization.1"
                     /domains="nrpspksdomains_IE274_RS38910_TIGR01720.1"
                     /locus_tags="IE274_RS38910"
                     /monomer_pairings="Leu -> D-Leu"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1912..1974)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0029"
                     /evalue="2.40E-11"
                     /label="NRPS-E6"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2612"
                     /protein_start="2591"
                     /score="35.7"
                     /tool="antismash"
                     /translation="VKEQLHAVPGNGIGYGLLRYL"
     misc_feature    complement(1920..1922)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        complement(2035..2982)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_Epimerization.1"
                     /evalue="2.20E-130"
                     /label="IE274_RS38910_Epimerization.1"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2571"
                     /protein_start="2255"
                     /score="427.7"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHFLVDG"
                     /tool="antismash"
                     /translation="MPLLPIGRYLLELADQGGGFGRFAMSMEVELPGGIDERGLAATLG
                     AVLDHHDILRSRLVVTADGDAGLHVTAPGSVDPAALIHRVACDGRWDEAAWRDRAAAEL
                     DRATGRLDPAAGVMAQFVWCDPGPGTPGRLLLVLHHFLVDGVSWRVLLPDLAAAWTDIR
                     DGRTPRLEPVATSVRRWSHALAEEATSPRRTAELDHWRAVVSGPDPDLGSRAFDPAVDV
                     SGTVAHVSVELPVAATEALLTTVPAAFRTGVNDGLLTAFGLALAKWRRARGVDEPSALI
                     RLEGHGREEEAAPGADLSRTVGWLTSMFPVRVDVG"
     CDS_motif       complement(2044..2094)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0028"
                     /evalue="6.20E-08"
                     /label="NRPS-E5"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2568"
                     /protein_start="2551"
                     /score="24.8"
                     /tool="antismash"
                     /translation="DLSRTVGWLTSMFPVRV"
     CDS_motif       complement(2518..2583)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0027"
                     /evalue="3.70E-09"
                     /label="NRPS-E2"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2410"
                     /protein_start="2388"
                     /score="28.9"
                     /tool="antismash"
                     /translation="LLLVLHHFLVDGVSWRVLLPDL"
     CDS_motif       complement(2809..2910)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0026"
                     /evalue="8.00E-05"
                     /label="C2_DCL_024-062"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2313"
                     /protein_start="2279"
                     /score="14.9"
                     /tool="antismash"
                     /translation="MSMEVELPGGIDERGLAATLGAVLDHHDILRSRL"
     aSDomain        complement(3034..3234)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_PCP.3"
                     /evalue="1.20E-23"
                     /label="IE274_RS38910_PCP.3"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2238"
                     /protein_start="2171"
                     /score="75.0"
                     /tool="antismash"
                     /translation="EEILAAVYAEVLGLDRVGVDDDFFAIGGDSIRSIQVVSRARAQGV
                     EVTPRQIFECRTVAELADVAAA"
     CDS_motif       complement(3283..3309)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0025"
                     /evalue="8.30E+00"
                     /label="NRPS-A_a10"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2155"
                     /protein_start="2146"
                     /score="0.6"
                     /tool="antismash"
                     /translation="GKLDHSALP"
     CDS_motif       complement(3532..3597)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0024"
                     /evalue="4.80E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2072"
                     /protein_start="2050"
                     /score="31.6"
                     /tool="antismash"
                     /translation="YVSRDDAQVKVRGFRIEPGEIE"
     aSDomain        complement(3562..4743)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_AMP-binding.2"
                     /evalue="3.00E-93"
                     /label="IE274_RS38910_AMP-binding.2"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2062"
                     /protein_start="1668"
                     /score="304.6"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="FAQRAAATPEATALVCEDQKWSYAELAARAGRLAAELAGRGVGPE
                     TVVGLALPRSADLVVAMLGIWRAGGAYLPIDPKYPSSRLDFILADARPALVLTDASAVC
                     VLTGADVPHLLVDDLDLDPDAHDTPARDREPAVVRPLNAAYVMYTSGSTGTPKGVTVTH
                     RDVVNGVLRLAEAVDIHAGTRMLAGTSVNFDVSVFEIVTTLAAGGTVELVRDVLVIGER
                     GGWDGGVISTVPSVFAELVDQIGDRIEADAVVFAGEALPAALVRSAHEALPGVRVVNAY
                     GQTESFYATTFTADTERTGGTSAPIGTPLGNMRTYVLGSGLAPVPVGVVGELYVAGNVA
                     RGYHGRASLTAERFLPDPFGPPGARMYRTGDRARWTSQGHLEYVSRDDAQVKVR"
     CDS_motif       complement(3682..3768)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0023"
                     /evalue="1.90E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="IE274_RS38910"
                     /protein_end="2022"
                     /protein_start="1993"
                     /score="42.6"
                     /tool="antismash"
                     /translation="PVGVVGELYVAGNVARGYHGRASLTAERF"
     CDS_motif       complement(4255..4314)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0022"
                     /evalue="2.20E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1831"
                     /protein_start="1811"
                     /score="29.3"
                     /tool="antismash"
                     /translation="AYVMYTSGSTGTPKGVTVTH"
     CDS_motif       complement(4516..4557)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0021"
                     /evalue="1.70E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1744"
                     /protein_start="1730"
                     /score="14.9"
                     /tool="antismash"
                     /translation="LGIWRAGGAYLPID"
     CDS_motif       complement(5083..5202)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0020"
                     /evalue="3.70E-24"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1555"
                     /protein_start="1515"
                     /score="77.2"
                     /tool="antismash"
                     /translation="RAKSLAAYDHQDVPFERLVEELNPERSTAYAPLFQVMFAW"
     aSDomain        complement(5242..6147)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="7.70E-110"
                     /label="IE274_RS38910_Condensation_LCL.2"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1502"
                     /protein_start="1200"
                     /score="358.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIACDG"
                     /tool="antismash"
                     /translation="ERAPLSYAQRRLWFIDRFEGPSATYNAPFPLRLSGELDAVALAAA
                     MRDVVARHESLRTVFAEDEDGVPCQRVLPAHEAQLDVAVVDVEPGGVAAALAEFAAHRF
                     DLAQEIPVKAALLRLGAADHVLVWLVHHIACDGASLAPMARDLNTAYTARLADGLPQWT
                     ELPAQYVDYTLWQRDLLGDENDPNSLLSTQVAYWREELADIPQPLQLPTDRPRPRRASH
                     DGAAVEFSVDNRIMSAVADLARTHGATESMVLEAALAVLLHGLGGGDDITIGSPIANRT
                     DENLTDLVGFFVNTWVLRTRL"
     CDS_motif       complement(5251..5340)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0019"
                     /evalue="3.20E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1499"
                     /protein_start="1469"
                     /score="45.2"
                     /tool="antismash"
                     /translation="DITIGSPIANRTDENLTDLVGFFVNTWVLR"
     CDS_motif       complement(5608..5646)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0018"
                     /evalue="6.70E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1380"
                     /protein_start="1367"
                     /score="12.4"
                     /tool="antismash"
                     /translation="QYVDYTLWQRDLL"
     CDS_motif       complement(5695..5760)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0017"
                     /evalue="5.40E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1351"
                     /protein_start="1329"
                     /score="25.4"
                     /tool="antismash"
                     /translation="VHHIACDGASLAPMARDLNTAY"
     CDS_motif       complement(5965..6081)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0016"
                     /evalue="4.10E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1261"
                     /protein_start="1222"
                     /score="51.3"
                     /tool="antismash"
                     /translation="ATYNAPFPLRLSGELDAVALAAAMRDVVARHESLRTVFA"
     CDS_motif       complement(6103..6132)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0015"
                     /evalue="2.50E-02"
                     /label="C1_LCL_004-017"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1215"
                     /protein_start="1205"
                     /score="7.6"
                     /tool="antismash"
                     /translation="SYAQRRLWFI"
     aSDomain        complement(6190..6396)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_PCP.2"
                     /evalue="9.20E-30"
                     /label="IE274_RS38910_PCP.2"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1186"
                     /protein_start="1117"
                     /score="94.7"
                     /tool="antismash"
                     /translation="EETLAGVFADVLGADRVGIDDSFFDLGGHSLLATRLVSRIRAALG
                     VELPIRTVFAAPTVAALAERLDQG"
     aSModule        complement(6190..9324)
                     /complete
                     /domains="nrpspksdomains_IE274_RS38910_Condensation_LCL.1"
                     /domains="nrpspksdomains_IE274_RS38910_AMP-binding.1"
                     /domains="nrpspksdomains_IE274_RS38910_PCP.2"
                     /locus_tags="IE274_RS38910"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(6361..6414)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0014"
                     /evalue="1.60E+01"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1129"
                     /protein_start="1111"
                     /score="-1.8"
                     /tool="antismash"
                     /translation="RPRTPAEETLAGVFADVL"
     CDS_motif       complement(6448..6474)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0013"
                     /evalue="1.90E+00"
                     /label="NRPS-A_a10"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1100"
                     /protein_start="1091"
                     /score="2.3"
                     /tool="antismash"
                     /translation="GKVDHRALP"
     CDS_motif       complement(6691..6756)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0012"
                     /evalue="1.30E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1019"
                     /protein_start="997"
                     /score="36.5"
                     /tool="antismash"
                     /translation="YHGRADTQVKIRGFRIEPGEVE"
     aSDomain        complement(6721..7917)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_AMP-binding.1"
                     /evalue="1.30E-105"
                     /label="IE274_RS38910_AMP-binding.1"
                     /locus_tag="IE274_RS38910"
                     /protein_end="1009"
                     /protein_start="610"
                     /score="345.3"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="AEHVTAAPDRLAVVSAEQELTYRELDARANRLARVLIAHGVRRDS
                     VVGLALPRSADQVVAVIAILKAGGCYLPLDPEYPAERLAYMLRDSAPPLVVTDTENASS
                     LPDNVCPALVLDEPDTAAAVARESAEPLTVAGHPDQLAYVMYTSGSTGRPKGVGVTQRG
                     VVRLSAESGYREGAHERVLTQCAQAFDCNTYEQWFPLLKGGTLVIAPPGHVDTATLARL
                     VTEHRLTAVMSSSGIFRVIAEELPEAFAGLRDTLTGGEVVPRSAYENILAACPGTRVIN
                     GYGPTEATMACTFHGVTAHEGIGVLGEIGRPMDNTRIYLLDSALRPVLPGVPGELYIAG
                     DRLARGYLGRFDLTAERFVACPFGDTPGERMYRTGDIATWTPEGSLVYHGRADTQVKIR
                     "
     CDS_motif       complement(6844..6930)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0011"
                     /evalue="8.40E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="IE274_RS38910"
                     /protein_end="968"
                     /protein_start="939"
                     /score="46.9"
                     /tool="antismash"
                     /translation="PGVPGELYIAGDRLARGYLGRFDLTAERF"
     CDS_motif       complement(7438..7494)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0010"
                     /evalue="1.70E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="IE274_RS38910"
                     /protein_end="770"
                     /protein_start="751"
                     /score="26.6"
                     /tool="antismash"
                     /translation="AYVMYTSGSTGRPKGVGVT"
     CDS_motif       complement(7693..7731)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0009"
                     /evalue="1.70E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="IE274_RS38910"
                     /protein_end="685"
                     /protein_start="672"
                     /score="14.9"
                     /tool="antismash"
                     /translation="AILKAGGCYLPLD"
     CDS_motif       complement(8260..8376)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0008"
                     /evalue="3.40E-24"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE274_RS38910"
                     /protein_end="496"
                     /protein_start="457"
                     /score="77.3"
                     /tool="antismash"
                     /translation="TKSLAAYDHQDVPFERLVEALNPERSTAYAPLFQVMFAW"
     aSDomain        complement(8419..9324)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.40E-111"
                     /label="IE274_RS38910_Condensation_LCL.1"
                     /locus_tag="IE274_RS38910"
                     /protein_end="443"
                     /protein_start="141"
                     /score="364.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIACDG"
                     /tool="antismash"
                     /translation="ERVPLSFAQRRLWFIDRFEGLSATYNAPFPLRLKGELDADALRAA
                     VRDVVARHESLRTVFAEDEDGVPFQRVLPAAEAHVDVPVLEVEPAGLERAIVDFMSHRF
                     DLAAEIPVKAVLLRLGAADHMLVWLVHHIACDGASMAPMARDLTTAYTARVADGLPQWT
                     ELPAQYVDYTLWQRDLLGDGNDPDSLLSAQVAYWQEELADIPQPLQLPTDRPRPAEASH
                     RGDSLEFTLDAELMTTVDQLARTHGATSSMVLEAALAVLLHSLGGGDDITIGSPIANRT
                     DEGLADLVGFFVNTWVLRTRL"
     CDS_motif       complement(8428..8517)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0007"
                     /evalue="1.80E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE274_RS38910"
                     /protein_end="440"
                     /protein_start="410"
                     /score="49.2"
                     /tool="antismash"
                     /translation="DITIGSPIANRTDEGLADLVGFFVNTWVLR"
     CDS_motif       complement(8785..8823)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0006"
                     /evalue="6.70E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE274_RS38910"
                     /protein_end="321"
                     /protein_start="308"
                     /score="12.4"
                     /tool="antismash"
                     /translation="QYVDYTLWQRDLL"
     CDS_motif       complement(8872..8937)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0005"
                     /evalue="7.20E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE274_RS38910"
                     /protein_end="292"
                     /protein_start="270"
                     /score="28.2"
                     /tool="antismash"
                     /translation="VHHIACDGASMAPMARDLTTAY"
     CDS_motif       complement(9142..9258)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0004"
                     /evalue="6.20E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE274_RS38910"
                     /protein_end="202"
                     /protein_start="163"
                     /score="57.2"
                     /tool="antismash"
                     /translation="ATYNAPFPLRLKGELDADALRAAVRDVVARHESLRTVFA"
     CDS_motif       complement(9280..9309)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0003"
                     /evalue="2.10E-02"
                     /label="C1_LCL_004-017"
                     /locus_tag="IE274_RS38910"
                     /protein_end="156"
                     /protein_start="146"
                     /score="7.8"
                     /tool="antismash"
                     /translation="SFAQRRLWFI"
     aSDomain        complement(9370..9573)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38910_PCP.1"
                     /evalue="1.00E-26"
                     /label="IE274_RS38910_PCP.1"
                     /locus_tag="IE274_RS38910"
                     /protein_end="126"
                     /protein_start="58"
                     /score="84.9"
                     /tool="antismash"
                     /translation="EELLCGLFAEVLGLDRVGIDDSFFDLGGHSLLATRLVSRVRAVLG
                     VELPIRMVFTAPTVVDLVEHLGQ"
     CDS_motif       complement(9550..9615)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0002"
                     /evalue="1.10E+01"
                     /label="C5_DCL_263-294"
                     /locus_tag="IE274_RS38910"
                     /protein_end="66"
                     /protein_start="44"
                     /score="-1.4"
                     /tool="antismash"
                     /translation="FAAVATGRTPRTPQEELLCGLF"
     CDS_motif       complement(9625..9651)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE274_RS38910_0001"
                     /evalue="2.90E-01"
                     /label="NRPS-A_a10"
                     /locus_tag="IE274_RS38910"
                     /protein_end="41"
                     /protein_start="32"
                     /score="4.4"
                     /tool="antismash"
                     /translation="GKLDRKALP"
ORIGIN
        1 cccgcgccag ccgattcgac cgctcatcca gatccgcgta cgacaactcc tcctccccgg
       61 agaccagcgc gacccgctcc ggatcggcgg tcgcctgctc ctggaacacc tcggcgatgc
      121 cgcacccggc ccgctcccgg ccggtgtcgt tgaaccgctc caggacctgg tcgcgttccg
      181 tcccgtcgag gaggtccagg cgggccaccg ggaggtgcgg gtcggcggcg acccgttcga
      241 ggagctgccc gaaccgggcg gcgatccgtt ccgccgcggc cttcgggaac aggttctcct
      301 ggtactggag ttccgcgcgc aggtgcggct ccgcggcggc gaccacagcg agcgggtagt
      361 gggtgccggt ggtcgcgcgc acaccggtga gggcggcacc ggccaccgcg tgcgcttcct
      421 cgagccccgc ctcgtccacc gggtacgact ccaggacgac catggtgtcg aacagggtcg
      481 ggaggcccat ggtctcgtgg atccggctga ggctgtggtg atggtggtcg agcagtggtg
      541 tctggcgctc ctggagggag gtcagggtct gcgcgagcgt ggcgtcgggc gcccacgtga
      601 cgcgtaccgg cagggtgttg atgaacaggc cgaccatctc gtcgaccccc gccacctgcg
      661 gcgggcgccc ggagacggtc gtgccgagca ccacgtcccg gcggtccgtc aggtagccga
      721 ggagaaggcc ccaggttccc tgcagcaggg tgttgagggt gacgcccagc tcgctcgccc
      781 tgcgggagag ctcccgcgcg gccgggtcgg gcagcggcac ctcgatctgg cccaggccct
      841 ccgactcggt ggtggccccg gcccgggtgg cgaggagcgt cggctccgtg acgccgtcca
      901 gctcccgcgc ccaggcgcgg gccgccgcct cctcgtcctg ctgggacagc cagacgagga
      961 agtcacggta cgggcgcacc cggggcagtg ccgagggggc cccgccggag ccgtagaggc
     1021 gcagcagttc ccgtagcagg tggggtatcg accagccgtc gtacagggcg tggttggcgg
     1081 tgaagaccag ctccgtgcgg tccgggcccc tgcggatcag ggagaggcgc agcagcggcg
     1141 gcctgtccgg ctcgaagtgg acggcctggt cggcggcgag gaggcgctcg aaggccgcgt
     1201 cccgttcggc ctcgtcgagg tcggtcaagt ccacggcctg ccagggcagt tcgacgtctt
     1261 cgaggacgag ctggacctgc tctccggccg cgctcgtgac gaaggcggtc cggagtgcgg
     1321 cgtgccggtc caggagggcc tgcccggcgg tccgcatgcg ctccgggtcg acgttcccgg
     1381 acaggctgaa ggcgagctgg gtgtggtacg cgtcgaacga cgactccgcg agcatgccgt
     1441 ggaagaggat gcccgactgt gtcgccgtca ccggccacac gtcggccacc gcgcccgggt
     1501 accggtcgtg ccaccggtcc aggtcgcgct ggctcacccg caccagcggc acgtcggacg
     1561 gcgtgaggcc gcccgatccg ggtgcggcgg cgtgcagggc gaggttttcg agcgccgagc
     1621 gccacagctc gatcagctcg cgcgcgtcat cgtgggcgag cagcccgctc gggaagctga
     1681 gctgcgcggt gagccgcggg ccctgcgcgg tgtcggagac cagcgaggtg acctcgatcg
     1741 tcgccatcgc gggcatgtcc gcgtcgagtt cggcgccccg caggccgttg tcgtcggcgg
     1801 gcgtccagcc gaggccgcgc agctcctcgg gcgtgtcctc ggcgaaccgg cccaggtagt
     1861 tgaaggagat ctggccgccc gtggccggct tgaggatgtc ggcggtctcg gggttgaggt
     1921 aacggagcag gccgtagccg atgccgttcc ccggtacggc gtgcagctgt tccttgacgg
     1981 ccttcaggac ctggcccgcg gcccggccac cgctgaaggc gtcctccaga tcgacgccgc
     2041 ccacgtccac gcgcacaggg aacatgctgg tgagccagcc cacggtgcgg gacagatcgg
     2101 ccccgggcgc cgcctcctcc tcgcggccgt ggccctcgag ccggatcagc gccgacggct
     2161 cgtcgacacc gcgcgcccgc cgccacttcg cgagcgcgag cccgaaggcg gtgaggaggc
     2221 cgtcgttgac gccggtgcgg aaggcggcgg gcacggtggt caggagcgcc tcggtggccg
     2281 cgaccggcag ttccacggac acgtgcgcca ccgtgccgga cacgtcgacg gcggggtcga
     2341 aggcccggga gccgaggtcg gggtcggggc cggacacgac agcccgccag tggtcgagct
     2401 cggcggtgcg ccgtgggctc gtggcctcct cggcgagggc gtgggaccac cgtcgtacgg
     2461 aggtggcgac cggttcgagc cggggtgtcc ggccgtcccg gatgtcggtc caggccgcgg
     2521 cgaggtcggg cagcaggacg cgccaggaca cgccgtcgac gaggaagtgg tgcaggacga
     2581 ggaggaggcg tccgggggtg cccgggcccg ggtcgcacca gacgaactgg gccatgaccc
     2641 cggccgccgg gtcgaggcgg ccggtggccc ggtcgagctc ggcggcggcg cggtcgcgcc
     2701 aggccgcctc gtcccagcgg ccgtcgcagg cgacccggtg gatgagggcg gccgggtcga
     2761 cggatcccgg cgccgtgacg tgcaggcccg cgtcgccgtc agcggtgacg acgagccggg
     2821 agcgcaggat gtcgtggtgg tcgaggaccg cgcccagggt cgcggcgagc ccccgctcgt
     2881 cgatgccgcc cggcagctcg acctccatgg acatcgcgaa ccggccgaag ccgccgccct
     2941 gatccgcgag ttccagcagg tagcggccga tgggcaggag cggcatccag ccgacgccgc
     3001 cgccgtccag ctccgccagg acagtggcct gtcgtccggc cgccgcgacg tcggccagct
     3061 cggccacggt gcggcactcg aagatctgcc gcggggtgac ctcgacgccc tgtgcgcggg
     3121 cccgcgagac gacctggatg gagcggatgg agtcgccgcc gatggcgaag aagtcgtcgt
     3181 cgacgccgac ccggtccagg cccaggacct cggcgtacac ggcggccagg atctcctcgg
     3241 cggccgtgcg cggtgcgtgg tactcgccgc cggtgcactc cggctccggc agcgccgagt
     3301 ggtccagctt gccgttcggg gccaggggca gccggtccag ggtcacgaac accgacggca
     3361 ccatgaactc cggcagccgt tcggccacga aggcgcgcag cgccttcgcg gagacaccgg
     3421 cgccgatgcc cgcgtcggcg gcgggtacga cgtagccgac cagttgcgtg ctgccgcgga
     3481 cgtcccgggt gccgacgacc gcctgcgcca cgcccgcgtg tgcggtgagt gccgcctcga
     3541 tctcgcccgg ttcgatgcgg aagccgcgca ccttgacctg tgcgtcgtcg cggctcacgt
     3601 attccaggtg gccttgggag gtccagcggg cccggtcacc ggtgcggtac atccgtgccc
     3661 ccggcgggcc gaaggggtcc gggaggaagc gttcagcggt cagcgacgcc ctcccgtggt
     3721 agccgcgggc cacgttgccc gcgacgtaca gctcgcccac gacaccgacg ggtacggggg
     3781 cgagcccgga gccgaggaca taggtgcgca tgttgcccag gggggtgccg atgggggcgc
     3841 tcgtgccgcc ggtccgctcc gtgtcggcgg tgaaggtggt ggcgtagaac gactccgtct
     3901 ggccgtaggc gttgacgacg cgcactccgg gcagggcctc gtgggccgac cgcaccagcg
     3961 cggcgggcag cgcttcaccg gcgaagacga cggcgtcagc ctcgatcctg tcgccgatct
     4021 ggtccaccag ttcggcgaag accgagggca cggtgctgat cacgccgccg tcccagccgc
     4081 cgcgctcacc gatcacgagg acgtcccgga ccagttcgac ggtgcctccg gccgcgaggg
     4141 tggtgacgat ctcgaacacc gacacgtcga agttcacgga ggtgcccgcg agcatccgcg
     4201 tgcccgcgtg gatgtccacc gcctcggcca gccgcagcac gccgttgacg acgtcccggt
     4261 gcgtgaccgt cacgcccttc ggcgtgccgg tcgagccgga ggtgtacatc acgtatgcgg
     4321 cgttcaacgg ccgcaccacc gcgggctccc ggtcccgcgc cggggtgtcg tgggcgtccg
     4381 ggtccaggtc caggtcgtcg acgagcaggt gcgggacgtc ggcgcccgtg agcacgcaca
     4441 ccgcggaggc gtccgtcagg accagcgcgg gccgggcgtc cgccaggatg aagtccagcc
     4501 tggagctcgg gtacttcggg tcgatcggca gatacgcgcc gcccgcccgc cagatcccca
     4561 gcatcgccac gaccaggtcc gccgagcgcg gcagcgcgag ccccacgacc gtctccggac
     4621 ccaccccgcg gccggccagc tcagcggcga gccgcccggc ccgggcggcc agttccgcgt
     4681 acgaccactt ctggtcctcg cagaccagtg ccgtggcctc gggggtggcc gccgcccgtt
     4741 gcgcgaacag ctcggcggcc gtcagctccg gcgtcgcggc ggcggtgtcg ttgaaggcgg
     4801 agagcacgag ctcccgctcg tccggctcga cgagttcgac gccgcgcacg tgctggcgcg
     4861 ggtcggcggt gagccggtcg aggaggcgga cgtagcggtc cgccagcttg cggaccgtgg
     4921 ggtcgtcgaa gaggtcggtg gcgtactcca acgagccgac cactccgccc agttccggca
     4981 ggtcgaccat gtggaaggtc aggtcgaact tcgccgagtc gctgtacgca cgctcgaact
     5041 ccaccccgag acccggcagt tcgaagccgt cgaggtcgac gttctgccag gcgaacatca
     5101 cctggaacag cggcgcgtac gcggtcgacc gctccgggtt gagctcctcc acgagccgtt
     5161 cgaagggcac gtcctggtgg tcgtaggccg ccagggactt ggcccggacc tggtcgagca
     5221 cgtccgcgaa ggtcgggttg cccgccagcc gggtccgcag gacccaggtg ttgacgaaga
     5281 agccgaccag gtcggtgagg ttctcgtcgg tgcggttggc gatcggcgag ccgatcgtga
     5341 tgtcgtcacc gccgccgaga ccgtgcagca gcacggccag agccgcctcc aggaccatcg
     5401 actccgtcgc cccgtgggtg cgggcgaggt ccgccacggc gctcatgatc cggttgtcga
     5461 cggagaactc gacggcggcg ccgtcgtgac tggcccgccg cgggcggggg cggtcggtgg
     5521 gcagttggag gggctgcggg atgtccgcca gctcctcgcg ccagtacgcg acctgagtgg
     5581 acaggaggct gttcgggtcg ttctcgtcgc cgagcaggtc gcgctgccac agcgtgtagt
     5641 cgacgtactg cgcgggcagt tccgtccact gcgggagtcc gtccgcgagc cgggccgtgt
     5701 aggcggtgtt gaggtcgcgg gccatcgggg ccagcgacgc gccgtcgcac gcgatgtggt
     5761 gcaccagcca caccagcacg tggtccgccg cgcccaggcg cagcagtgcc gccttgaccg
     5821 ggatctcctg cgccaggtcg aagcggtgcg cggcgaactc cgcgagggcg gccgccaccc
     5881 caccgggttc gacgtccacg accgcgacgt ccaactgggc ctcgtgcgcg ggcaggaccc
     5941 gctggcaggg cacaccgtcc tcgtcctccg cgaacacggt ccgcaggctc tcgtgccgcg
     6001 ccaccacgtc ccgcatggcg gcggcgagtg ccaccgcgtc gagctccccg ctcaaccgca
     6061 gcgggaaggg cgcgttgtac gtggccgacg gcccctcgaa ccggtcgatg aaccacaggc
     6121 ggcgctgcgc gtacgacagc ggcgcgcgct ccggccgcgg ctcgaccctg gtcagcggcg
     6181 gccgtacccg ctctccctgg tcgaggcgtt cggccagcgc ggccacggtg ggcgcggcga
     6241 agacggtacg gatgggcagc tcgacgccga gggccgcgcg gatgcggctg accagacggg
     6301 tggcgaggag ggagtggccg cccaggtcga agaagctgtc gtcgatgccg acccggtcgg
     6361 cgccgagcac gtcggcgaag acgcccgcca gggtctcctc cgccggggtg cggggcctgc
     6421 ggtagggctt ggcgctggtg aactcgggtt ccggcagcgc cctgtggtcc accttgccgc
     6481 tggggctcag cgggatgctg tccagggaca cgaacgcgga cggcaccatg tggtccggga
     6541 gttgccccgc cgcgaaggag cgcaggtcgg cgaggtcggt cccgctctcc gtgacgctgg
     6601 ggacggtgta ggcgatgagc tgcttgccga actccgcgct gtcgcgggtg atcaccacgg
     6661 cctgggcgac ggcaccgtgc cgggtcaggg ccgcctcgac ctcgccgggt tcgatgcgga
     6721 agccgcggat cttgacctgg gtgtcggcgc ggccgtggta gaccaggctg ccctccggtg
     6781 tccaggtggc tatgtcgccg gtgcggtaca tgcgctcgcc gggggtgtcg ccgaaggggc
     6841 aggccacgaa gcgttcggcg gtcaggtcga agcggccgag gtagccgcgg gcgaggcggt
     6901 ctccggcgat gtacagctca ccgggtacgc ccggcaggac cgggcggagc gccgagtcca
     6961 ggaggtagat gcgggtgttg tccatcggcc ggccgatctc gccgagtacg ccgatcccct
     7021 cgtgcgccgt gaccccgtgg aaggtgcagg ccatcgttgc ctcggtcggc ccgtagccgt
     7081 tgatcacccg ggtgccgggg caggccgcga ggatgttctc gtaggcggag cgcggcacga
     7141 cctcgccgcc ggtcagggtg tcacgcagcc ccgcgaaggc ttccggcagt tcctcggcga
     7201 tcactcggaa gatgcccgac gacgacatga cagcggtcag ccggtgctcg gtcaccaggc
     7261 gcgcgagggt ggcggtgtcc acgtgcccgg gcggcgcgat gacgagcgtg ccgcccttga
     7321 ggagggggaa ccactgctcg taggtgttgc agtcgaaggc ctgcgcgcac tgcgtcagga
     7381 cccgctcgtg cgcgccctcg cggtacccgc tttccgcgct gagccgtacg acgccacgct
     7441 gggtgacgcc gacgcccttg ggcctgccgg tcgagccgga ggtgtacatc acgtacgcca
     7501 gttggtcggg atgcccggcg acggtcagcg gctcggccga ctcccgggcc accgcggccg
     7561 cggtgtccgg ctcgtccagg acgagagccg ggcagacgtt gtccggcagg ctcgacgcgt
     7621 tctcggtgtc ggtgaccacg aggggcggtg cggagtcgcg cagcatgtac gcaagccgct
     7681 cggccgggta ttcggggtcc aggggcagat agcaaccgcc cgccttgagg atggcgatga
     7741 cggccacgac ctggtcggcg gagcggggca gggcgagacc caccaccgag tcccgccgca
     7801 cgccgtgcgc gatgagcacg cgcgcgagcc ggttggcgcg ggcgtccagt tcccggtacg
     7861 tcagctcctg ctcggcggac acgaccgcga gccggtccgg ggccgccgtc acgtgctccg
     7921 cgatgacctc ggccatggag tgccgcacgg ccggcttcgc cgtgtcgttg aagccccgga
     7981 gcaccagctc acgctcgtcc gccgcgagca ggtccaccga cccgatccgt cggcgggggt
     8041 cggcggtgag ctgccggagc agccggacgt accggtccgc cagcttgcgg accgtggtcg
     8101 cgtcgaagag gtcggtggcg tactccaccg agcccacggc tccgccgggt ccggtctccg
     8161 ccatcaggtt gaaggtgagg tcgaacttcg acgactgact gtgcggccac tccagtgcga
     8221 cgtccaggcc gggcagcgcg aagtcctggc ggctgttgtt ctgccaggcg aacatcacct
     8281 ggaacagcgg cgcgtaggcc gtggagcgtt cggggttgag ggcctccacg agccgttcga
     8341 aggggacgtc ctggtggtcg taggcggcca aggacttggt gcgtacgtcg tcgagcacgt
     8401 ccgtgaacgt cgggttgccc gccaggcggg tgcgcaggac ccaggtgttg acgaagaagc
     8461 cgaccaggtc ggcgaggcct tcgtcggtgc ggttggcgat cggcgagccg atcgtgatgt
     8521 cgtcaccgcc gccgaggctg tgcaggagga cggccagggc tgcctccagg accatggagg
     8581 aggtcgctcc gtgcgtgcgg gcgagctggt cgaccgtggt catcagctct gcgtcgaggg
     8641 tgaattcgag ggagtcgccg cggtggctgg cctccgccgg gcgggggcgg tcggtgggca
     8701 gttggagggg ctgcgggatg tcggccagct cctcctgcca gtaggcgact tgggcggaga
     8761 ggaggctgtc ggggtcgttg ccgtcgccga ggaggtcgcg ctgccacagt gtgtagtcga
     8821 cgtactgggc gggcagttcc gtccactgcg ggagtccgtc tgcgacccgg gcggtgtagg
     8881 cggtggtgag gtcgcgggcc atcggggcca tcgaggcgcc gtcgcacgcg atgtggtgca
     8941 ccagccacac cagcatgtgg tccgccgcgc ccaggcgcag gagcaccgcc ttgaccggga
     9001 tctcggcggc caggtcgaag cgatgggaca tgaagtccac gatcgcgcgt tccagtccgg
     9061 ccggttcgac ctccaggacc ggcacgtcca cgtgggcctc ggccgcgggc aggacccgtt
     9121 ggaagggcac gccgtcctcg tcctcggcga agacggtccg caggctctcg tggcgggcca
     9181 ccacgtcccg cacagccgcc cgtagcgcgt cggcgtcgag ctcacccttc agccgcagcg
     9241 ggaacggcgc gttgtaggtg gccgagagcc cttcgaaccg gtcgatgaac cacaggcggc
     9301 gctgcgcgaa ggacagcggt acgcgttcgg ggcgcggggt gacgcgggtc agcggcgggc
     9361 gtacccgctc tccctgcccc aggtgttcca ccaggtccac gaccgtcggg gcggtgaaca
     9421 ccatgcggat gggcagttcc acgccgagca ccgcccgcac ccggctgacc aggcgcgtcg
     9481 cgagcaggga gtggccgccc aggtcgaaga agctgtcgtc gatgccgacc cggtccaggc
     9541 cgaggacctc ggcgaagagg ccacacagca gctcttcctg cggagtacgc ggggtccggc
     9601 ccgtcgccac cgcggcgaag tccggcgcgg gcagcgcctt gcggtccagc ttcccgttcg
     9661 ccgtcagcgg cagttcaggg atggccacca tggaggcggg caccaggtgt gcgggcaggc
     9721 ggtcggccag gtaggagcgc agttccgccg ggg
//
