LOCUS       NZ_BMVR01000041         9868 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces flavofungini strain JCM 4753 sequence41, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVR01000041
VERSION     NZ_BMVR01000041.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces flavofungini
  ORGANISM  Streptomyces flavofungini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650815.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:46:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,873
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,638
            CDSs (with protein)               :: 7,638
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 43 of 161
            Pseudo Genes (incomplete)         :: 137 of 161
            Pseudo Genes (internal stop)      :: 10 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9868
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVR01000041.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9868
                     /culture_collection="JCM:4753"
                     /db_xref="taxon:68200"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces flavofungini"
                     /strain="JCM 4753"
                     /submitter_seqid="sequence41"
                     /type_material="type strain of Streptomyces flavofungini"
     region          1..9868
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="azoxy-crosslink"
                     /region_number="1"
                     /rules="(azdH and LPG_synthase_C and azdO and vlmB)"
                     /tool="antismash"
     cand_cluster    1..9868
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(azdH and LPG_synthase_C and azdO and
                     vlmB)"
                     /kind="single"
                     /product="azoxy-crosslink"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9868
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[52:7794](+)"
                     /cutoff="20000"
                     /detection_rule="(azdH and LPG_synthase_C and azdO and
                     vlmB)"
                     /neighbourhood="10000"
                     /product="azoxy-crosslink"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      53..7794
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(azdH and LPG_synthase_C and azdO and
                     vlmB)"
                     /neighbourhood="10000"
                     /product="azoxy-crosslink"
                     /protocluster_number="1"
     gene            53..988
                     /locus_tag="IE274_RS38800"
                     /old_locus_tag="GCM10010349_79200"
     CDS             53..988
                     /NRPS_PKS="Domain: LPG_synthase_C (2-291). E-value:
                     1.7e-56. Score: 183.3. Matches aSDomain:
                     nrpspksdomains_IE274_RS38800_LPG_synthase_C.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azoxy-crosslink: LPG_synthase_C"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021445.5"
                     /locus_tag="IE274_RS38800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_79200"
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="WP_198898085.1"
                     /sec_met_domain="LPG_synthase_C (E-value: 2.9e-57,
                     bitscore: 183.3, seeds: 74, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSGTKYLNSQNVDGFLAYRMSGRRNLIGLCGPICDPGDRQQMIA
                     ELVEFARHERRHVMAVQISREEAEIYAKMGFIINQIGASFSLDITDYTLRGNRMSRVRS
                     MINRASREGIFVKEVDHAELQGTPALKAALDRIDERWLASKGQHAKKLDFLVGEREGPG
                     ASYRRVFAAMHGSEMIAYVSYSPVFGHRPGWLYDLTRRLPSAPPGTIEMIFSNALAAFR
                     AEGYSWVHLGFTPFAQMKEFEDIPNPASPATHKALSFLRKKGSLIYPSGAQEAFKLKWR
                     PQSIDPEYMASQGRVRIGAIWNLLRTVGAV"
     aSDomain        59..925
                     /aSDomain="LPG_synthase_C"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38800_LPG_synthase_C.1"
                     /evalue="1.70E-56"
                     /label="IE274_RS38800_LPG_synthase_C.1"
                     /locus_tag="IE274_RS38800"
                     /protein_end="291"
                     /protein_start="2"
                     /score="183.3"
                     /tool="antismash"
                     /translation="SGTKYLNSQNVDGFLAYRMSGRRNLIGLCGPICDPGDRQQMIAEL
                     VEFARHERRHVMAVQISREEAEIYAKMGFIINQIGASFSLDITDYTLRGNRMSRVRSMI
                     NRASREGIFVKEVDHAELQGTPALKAALDRIDERWLASKGQHAKKLDFLVGEREGPGAS
                     YRRVFAAMHGSEMIAYVSYSPVFGHRPGWLYDLTRRLPSAPPGTIEMIFSNALAAFRAE
                     GYSWVHLGFTPFAQMKEFEDIPNPASPATHKALSFLRKKGSLIYPSGAQEAFKLKWRPQ
                     SIDPEYMA"
     misc_feature    1136..1154
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 21.01"
                     /tool="antismash"
     gene            1179..2843
                     /locus_tag="IE274_RS38805"
                     /old_locus_tag="GCM10010349_79210"
     CDS             1179..2843
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888452.1"
                     /locus_tag="IE274_RS38805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_79210"
                     /product="aldehyde dehydrogenase"
                     /protein_id="WP_190120663.1"
                     /sec_met_domain="Aldedh (E-value: 2.8e-61, bitscore: 196.7,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKLGNSAGQFASDRNLRSYHSYINGRSASSESTEWVYVLDSRSV
                     LDDVFASLALKRNLEQGRVRVERYPESICGRVAKADRALVRQAVSAAAAAAPRWRAFPL
                     EVRLERFGALLYERLVRQAEEIVEILVREGNPLPLARWQVSGMLECFSAESIQFYRSQM
                     LQELHRDGRRIQIRRRPDGVVCVNPPQNAPLSSALLAVTSLMAGNTLVIRAPRGAPLGV
                     MYVLQDVVAPVLDEIDAPPGTLNALCGDPGPMLSTWLEEPSVNDIMYFGNSENGLRFEQ
                     RCISAGKKPILELAGNDIVAVWKDSDVDLAAEALSESFYGSGQLCMIPNQVLVHPEVAD
                     ALIEKLLGEIGNIRPGYPDEADVLLTPVLRNEKFFRCLDDALSKGAELICGGHAMQDDG
                     SRDPHGIFLEPTVISVNGLSKAREVDAVRQETFFPLLPLVIAESAPDPELLDAFIAYVN
                     SNECGLRNSVWARDTAVVDRFVNETVNGGLLKVNDSHISFLAGLPTHGGTGLTGGVFGE
                     ANYPMLRTSHVQGVSISAGSRPREAVFDAWRKVFGTD"
     gene            2876..4015
                     /locus_tag="IE274_RS38810"
                     /old_locus_tag="GCM10010349_79220"
     CDS             2876..4015
                     /EC_number="1.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azoxy-crosslink: azdH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386601.1"
                     /locus_tag="IE274_RS38810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_79220"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_190120664.1"
                     /sec_met_domain="azdH (E-value: 6.5e-137, bitscore: 445.6,
                     seeds: 3, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSLDSARRTCELFLPGLLEALSDYPLDALERPDSPGIELFRKMG
                     GPGLVIPDSLGGRGAGPLQALEVTRALASAAPSLAVATTMHHFSVATLSCFADSLGGAG
                     GLGKRLKEISQQNLLVASGFAEGQPGQGILEPTMELQATRNGYLLRGSKKPCSLSGAMD
                     FLTASVVIPDGNGGHTTAVTLVPRDAPGLAVRPFWASWVLAGAQSDEVVLTDVLVQDEH
                     IMRTDPAHDAVSLDTLQSTGFVWFELLITASYLGIASQLVERVMNGPGGTASERAQLSM
                     RLETATLLLERTARMLVDGDSGPSVLVQALVARYGAQDAIGATVRQAVELLGGLAYIRS
                     GDIAYLAAASHGAGFHPPSRSSFAAPYLDYAAGGPLRLS"
     gene            4023..5318
                     /locus_tag="IE274_RS38815"
                     /old_locus_tag="GCM10010349_79230"
     CDS             4023..5318
                     /NRPS_PKS="Domain: Aminotran_3 (40-359). E-value: 6.9e-87.
                     Score: 283.5. Matches aSDomain:
                     nrpspksdomains_IE274_RS38815_Aminotran_3.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983724.1"
                     /locus_tag="IE274_RS38815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_79230"
                     /product="aspartate aminotransferase family protein"
                     /protein_id="WP_190120665.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9.4e-89, bitscore:
                     287.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPIITSAVNRDEIGRLYRTRLSKGRATLGELFGGHVEVESEGA
                     WITSQEGRRFLNCGGYGVLLMGARHPTVVRYVQEQLHRHPVASRVLLEPQAALAADALV
                     SKAPAGLERVHFACSGAEAVETAIKIARSTGRDQLIATHSGYHGKTMGALSLTGAAKYR
                     DPFHPLLPCVRHVPYGDAESLQAVLETLPGRACFVVEPVQGEAGVVLPPDGYLRSVQEL
                     CRAYDACFVLDEIQTGLGRLGSWWGADAEGLTPDILLSGKALGGGVLPVSAVLARPEAF
                     AAFDKDPYIHTSTFSGLPLAMAAVRGVLTALEEDHLVERARVLGDELLARLRTIAQHHL
                     GDGIVVEVRGRGLLIGIEFAIPGPAGDLLIELISHGVIANHSLNSSQVLRFTPPAVLSP
                     ADIQYLCEAFDHSCRALARQYAPISGGATRCA"
     aSDomain        4143..5099
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38815_Aminotran_3.1"
                     /evalue="6.90E-87"
                     /label="IE274_RS38815_Aminotran_3.1"
                     /locus_tag="IE274_RS38815"
                     /protein_end="359"
                     /protein_start="40"
                     /score="283.5"
                     /tool="antismash"
                     /translation="ESEGAWITSQEGRRFLNCGGYGVLLMGARHPTVVRYVQEQLHRHP
                     VASRVLLEPQAALAADALVSKAPAGLERVHFACSGAEAVETAIKIARSTGRDQLIATHS
                     GYHGKTMGALSLTGAAKYRDPFHPLLPCVRHVPYGDAESLQAVLETLPGRACFVVEPVQ
                     GEAGVVLPPDGYLRSVQELCRAYDACFVLDEIQTGLGRLGSWWGADAEGLTPDILLSGK
                     ALGGGVLPVSAVLARPEAFAAFDKDPYIHTSTFSGLPLAMAAVRGVLTALEEDHLVERA
                     RVLGDELLARLRTIAQHHLGDGIVVEVRGRGLLIGIEF"
     gene            5326..5826
                     /locus_tag="IE274_RS38820"
                     /old_locus_tag="GCM10010349_79240"
     CDS             5326..5826
                     /NRPS_PKS="Domain: Polyketide_cyc (8-129). E-value:
                     3.3e-19. Score: 61.4. Matches aSDomain:
                     nrpspksdomains_IE274_RS38820_Polyketide_cyc.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386599.1"
                     /locus_tag="IE274_RS38820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_79240"
                     /product="type II toxin-antitoxin system RatA family toxin"
                     /protein_id="WP_229910117.1"
                     /transl_table=11
                     /translation="MLVDLPNVSPSAGYAQVSDFARYPELASVVRTVTVHPLGDGQQKS
                     DWEVYFRNGILRWTEADDFHPHEHRISFRQTDGDFDSFAGTWHIISSDTGCRVRFAADF
                     DFGIPSMAGILDPVAERVIKETIARVVLGLFDNHGAVIGDPLLARAVTATALPVPGHVA
                     QVP"
     aSDomain        5350..5712
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS38820_Polyketide_cyc.1"
                     /domain_subtypes="Polyketide_cyc"
                     /evalue="3.30E-19"
                     /label="IE274_RS38820_Polyketide_cyc.1"
                     /locus_tag="IE274_RS38820"
                     /protein_end="129"
                     /protein_start="8"
                     /score="61.4"
                     /tool="antismash"
                     /translation="SPSAGYAQVSDFARYPELASVVRTVTVHPLGDGQQKSDWEVYFRN
                     GILRWTEADDFHPHEHRISFRQTDGDFDSFAGTWHIISSDTGCRVRFAADFDFGIPSMA
                     GILDPVAERVIKETIAR"
     gene            5826..6308
                     /locus_tag="IE274_RS38825"
                     /old_locus_tag="GCM10010349_79250"
     CDS             5826..6308
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azoxy-crosslink: azdO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628735.1"
                     /locus_tag="IE274_RS38825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_79250"
                     /product="hypothetical protein"
                     /protein_id="WP_190120667.1"
                     /sec_met_domain="azdO (E-value: 9.4e-66, bitscore: 210.0,
                     seeds: 3, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDAKNLFMTPTTAALVRAEYALGLLVALTLFLSHLHDVRWEPAIG
                     LFVYIDLIGYLPGAIQMRRSSTGDLHKAYYIAYNVTHSMLTQALVALLWMALLGPEWAL
                     LTLAIHLCGDRALFGNFLKPFGVRFEPHQHPAYHRFRREFSTAHQTSHRRPARDAS"
     gene            6347..6838
                     /locus_tag="IE274_RS40525"
                     /old_locus_tag="GCM10010349_79260"
     CDS             6347..6838
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1160: flavin reductase domain protein FMN-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013756.5"
                     /locus_tag="IE274_RS40525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_79260"
                     /product="flavin reductase family protein"
                     /protein_id="WP_372447299.1"
                     /transl_table=11
                     /translation="MTHALQRFAAGITVLTFDAPGGAAGVTVSTLTPVPGTCPMVSVAL
                     RRASKSLSELLTAEAFTANALAADQASLAQHFARRRRSRETSQLPPDTWAQAAGIPRLR
                     QATSWLECRVDQTASLRDHVLVIATVTAGIPGSGPPLIGFTGALHADLSGTTTARGSHP
                     "
     gene            6835..7794
                     /locus_tag="IE274_RS38835"
                     /old_locus_tag="GCM10010349_79270"
     CDS             6835..7794
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azoxy-crosslink: vlmB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890221.1"
                     /locus_tag="IE274_RS38835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_79270"
                     /product="VlmB-like protein"
                     /protein_id="WP_190120669.1"
                     /sec_met_domain="vlmB (E-value: 1.6e-133, bitscore: 434.0,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTADVPVEADWERAPSLMDGAMSVELTAAQCDLKYWLASVPHGT
                     LQGHVMGHADHVQPLAMMRTPGPLNTALKEELAYRSLAEEKATRVIGHLVALAPDLACL
                     EFYATQLLDEARHAMVFRSHLLDLGVSEERLHQEIQELAKQDSDAILDPLEAFGLRILR
                     DEGDFIGGVLALTVLVEGVLAPAAELSEQKWRIFDPAAADIERGAGIDEIRHLTVGSSI
                     IREHLLAHPQEKKRLLRLMHEGFALWDSLPVDDQLLRRENLFQQGIDEHSHLLGDYQVW
                     PGRRLADTSARERITTGQQWAQRMQSVRLAFAGLEGTP"
     gene            7791..8885
                     /locus_tag="IE274_RS38840"
                     /old_locus_tag="GCM10010349_79280"
     CDS             7791..8885
                     /EC_number="1.14.19.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) delta12_cd03507"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386596.1"
                     /locus_tag="IE274_RS38840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_79280"
                     /product="acyl-CoA desaturase"
                     /protein_id="WP_229910118.1"
                     /sec_met_domain="delta12_cd03507 (E-value: 2.5e-18,
                     bitscore: 55.4, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIPEMAEPPAHSHRPSPTQPANMKDITQLVKAAGLLEPQPLYYT
                     LKIFTNLGMLAAAWTAFALLGDSWWQLATAACLAFCFGQTSVIGHDAGHRQIGRTRKVA
                     NLVGYLHANVLTGTNFHWWIGHHTKHHNFPNHLSLDPDIQRRQVIFDPAHHARKKGTVK
                     RFIIRHQGWMFYVLIPLEGLRLNLAGYIAAYRYAAMRKATTDLILCTTHLVAYLTLLFS
                     ALPLGLAVSFLAVHQGLFGVYVGLLFAPNHKGMPLRDGQQEEQDWLPRQVLSSRNLAPN
                     RLTDFFYAGLNYQIEHHLFPTMPRRSLHKAQPLVKRYVLTHGLPYTETSPLRSYRDITR
                     FLTMVSHQVPERADSLTKARGGAQ"
     gene            8882..>9868
                     /locus_tag="IE274_RS38845"
     CDS             8882..>9868
                     /EC_number="2.3.1.180"
                     /GO_function="GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556347.1"
                     /locus_tag="IE274_RS38845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="WP_190120670.1"
                     /sec_met_domain="fabH (E-value: 1.1e-106, bitscore: 346.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRQAAVLSGLGSWLPPQVVTNDRLARQLDTSDEWIRRRTGIGQRH
                     IAGPGIATSDLAIEAGRRALKSAATDRADCVIVATTTPDHPCPATAPMVAAKLGLGTVP
                     AFDVAAVCAGFLYAGAVAEGLIAAGTVRTALVIGADTFSSILDPEDRTTRCVFGDGAGA
                     AYLRAGSPEEPGALGPFTLGSDGTGADMIMVGGGGSRQPLRAENIARSDPYFRMQGKAV
                     FRHAVERMSTAISTAIEGANWTLDDVEVFVTHQANLRIMQAVASHLGVPPERFPVHLDR
                     VGNTAAASVPLTLVDAASAGTLRAGQRTVLEVLTEVVDGVTVGLSARW"
ORIGIN
        1 cgttgcgctt cacagaccgt cactcgaacg gtggccacat cgaagaagct caatgaactc
       61 cggaacaaaa tatctcaact ctcagaatgt tgacggattc ctggcctatc ggatgagtgg
      121 gcgccggaat ctcatcgggc tctgcgggcc tatctgcgat cccggcgatc gccagcagat
      181 gattgccgag ttggtcgaat ttgcccgcca cgagagacgg catgtgatgg cggtgcaaat
      241 ctccagagag gaggctgaga tatacgccaa gatgggcttt atcatcaatc aaatcggggc
      301 atcattcagc ctcgatatca cggattacac cctccgagga aaccggatga gcagagtccg
      361 cagcatgatc aacagggcct ctcgggaggg tatctttgtg aaagaggtcg atcatgctga
      421 actgcaggga acgcccgccc tgaaagccgc tctcgatcgc atagacgaaa ggtggcttgc
      481 gtccaagggc caacatgcca agaagcttga cttcctcgtc ggcgagcgcg aggggccggg
      541 tgcttcgtat cggcgggtgt ttgcggcgat gcacggcagc gaaatgatcg cgtacgtgtc
      601 ttactcgcca gttttcggtc accggccagg gtggctgtat gacctcacgc gcaggctgcc
      661 cagtgcgcct cccggaacca ttgagatgat tttttcgaat gcacttgccg catttcgcgc
      721 cgaagggtac tcatgggttc atctcggctt cacccctttc gcacagatga aggaatttga
      781 ggacatcccg aatcccgcga gtccggccac tcataaggct ctctccttct tacgaaaaaa
      841 ggggagcctc atctaccctt ccggggcgca ggaagcattt aagctgaaat ggcgccctca
      901 gtccattgat cctgagtaca tggcatcaca gggccgggtg cgaatcgggg cgatatggaa
      961 tctactccga accgtcggag ccgtgtagcg gcggcgggcc cggcgtgccg gattcaatta
     1021 agctacgagg aagcactccg gtccttcctg ggcatactgg aatgcgaccc agatgccggc
     1081 cctttactat gtggtgacgt atcagactcg aagcctgcac catcccctcg cagctttccg
     1141 tatatctctc cagcgaaccc ggaggagtga tcaacttgat gtctaaactc ggcaattccg
     1201 cagggcaatt cgcaagtgac aggaatctgc gaagctacca ctcttatatc aatggccgct
     1261 cagcttcatc agaatccacc gaatgggtct atgtattaga ttcccgttcg gtgttggatg
     1321 acgtttttgc cagcctcgcg ctcaaacgca atctcgaaca ggggcgggtg agagttgaac
     1381 gttacccgga gtcgatctgc gggcgcgtcg ccaaggccga ccgcgccttg gtgcggcagg
     1441 cggtcagcgc ggcggctgct gccgctccgc ggtggagggc gttcccgctg gaggtgcggc
     1501 tggaacggtt cggtgccctg ctgtatgaac gcctcgtcag acaggctgaa gagatcgttg
     1561 aaatcctggt gcgagaaggg aatccgctgc ctctcgcccg gtggcaggtc tcgggcatgc
     1621 tggagtgctt ctcggcggaa tccatccaat tctatcgttc acagatgctg caagagcttc
     1681 accgcgacgg gaggaggata cagatccggc ggcggccaga cggtgtggtg tgtgtcaatc
     1741 cgcctcagaa cgccccgctt tccagtgccc tgcttgcggt gacgtcactc atggcgggaa
     1801 acacgctcgt catccgtgcc ccgcgcggtg ccccgctagg agtcatgtac gtgctccagg
     1861 atgtggtggc accggtgctt gatgagattg atgctccgcc tggcacactc aacgcactct
     1921 gcggcgaccc cggcccgatg ctttccacct ggctagaaga accgtcagtg aacgacatca
     1981 tgtacttcgg gaacagcgag aacggcctgc ggttcgagca gcgttgcatc agtgccggga
     2041 agaagccgat tcttgagctg gccggcaatg atatcgtggc cgtctggaaa gattctgacg
     2101 ttgatctggc ggccgaggcg ctcagcgaga gtttttatgg ctcgggccaa ctgtgcatga
     2161 tcccgaatca agtgttggtt cacccggaag tcgctgacgc actcatcgag aaactcctcg
     2221 gggagattgg aaatatccgg cccggctatc cggatgaagc tgacgttctg ctgactcccg
     2281 tcctgcgaaa tgaaaagttc ttccgctgcc tcgacgacgc cctttccaag ggagccgagc
     2341 tgatctgtgg tggacatgcc atgcaggacg atggaagccg agacccgcat gggatcttct
     2401 tggagcccac tgtgatctcc gtcaacggtc tcagcaaggc cagagaggtg gacgcggtac
     2461 ggcaggaaac cttcttcccc ctgcttccgc tcgtgattgc agaaagcgca cccgaccctg
     2521 aactgctgga cgcattcatc gcgtatgtga actcgaacga atgcgggctg cgtaactctg
     2581 tctgggcaag ggacactgca gtagtagacc ggtttgtcaa cgaaacggtc aacggcgggt
     2641 tactgaaggt gaatgactcg cacatttcgt ttctggccgg cctgccgacg cacggtggaa
     2701 cagggcttac cggcggagtt tttggtgagg ccaactaccc catgctccgc acctcccatg
     2761 tccagggagt aagcatcagc gcgggttccc gtccgcggga ggcagtcttc gatgcgtgga
     2821 gaaaagtatt cggcacggac taagcgatct catcactctg cagaaggggc gaagaatgcg
     2881 ttcgctagat tcggcacgtc gcacctgtga attgttcctg cccggactcc tcgaggcgct
     2941 gtccgactac ccgctcgatg cccttgagcg tcccgatagt cccggcattg agctattccg
     3001 aaaaatgggt ggtcccggtc tggtgatccc ggattccctc ggggggcgtg gcgccggtcc
     3061 gctccaagct ttagaagtga cacgtgccct ggcctccgcc gcgccgtcgc tggcggtcgc
     3121 gacgaccatg catcacttct ccgtggctac cctgtcatgc tttgctgaca gtctcggagg
     3181 cgcgggaggt ctaggcaaac gcctcaagga aatctcccag cagaatctgc tcgtcgcgtc
     3241 cggcttcgcc gaaggccagc cgggccaggg catcctggaa cccaccatgg agctgcaggc
     3301 caccaggaac ggctacctgc tgcgcggatc gaagaagccc tgcagtttgt cgggtgccat
     3361 ggacttcctc acggcaagcg tcgtcatccc tgatggaaac ggcggtcaca ccacagcagt
     3421 gaccctggtg ccccgtgacg cgccgggcct ggcggtgcgc cccttctggg cgagctgggt
     3481 gctggcaggg gcgcagagtg acgaagtggt gctcaccgat gtcctggtgc aggacgagca
     3541 catcatgcgc accgatcccg cccatgacgc ggtgagcctc gacaccctgc aaagcacggg
     3601 tttcgtctgg ttcgaactgc tcatcaccgc gtcgtatctc gggatagcca gccagctcgt
     3661 cgagcgggtc atgaacggac cgggcggaac agcatcggag cgggcccagc tgtctatgcg
     3721 gctcgagacg gcgacgctgc tcctggagag gacagcccgc atgctcgtgg acggcgactc
     3781 gggcccttca gtactcgtcc aggcgctggt ggcgcgctac ggcgcgcagg acgccatcgg
     3841 ggcaacggtc cgccaggccg tagagctgct gggcggcctg gcctacatca ggtcagggga
     3901 catcgcctac ctggccgctg cgtcgcatgg ggcgggcttc catccgccct cgcgcagcag
     3961 cttcgcagct ccctacctcg actacgcggc cggcgggcct ctccgactgt cataggagtg
     4021 tcatgaccgc accaattatt acgagcgctg tgaaccggga cgagatcggc cgcctgtacc
     4081 ggacgcggct gagcaagggc cgcgccacct tgggggaact cttcggcggg catgtcgagg
     4141 tggagtccga gggggcctgg attaccagcc aagaaggcag acgctttctc aactgcggcg
     4201 gatacggggt cttgttgatg ggggcgaggc accccacggt ggtgcgttac gtccaggagc
     4261 agctccatcg ccaccctgtg gccagccgtg tcctgctgga gccgcaggcg gccctggccg
     4321 cggatgccct ggtgagcaag gcccccgcgg gactcgagcg cgtgcatttc gcctgctctg
     4381 gcgcggaagc cgtcgaaacc gctattaaga tcgcccgcag tacgggcagg gatcagctca
     4441 tcgccacgca cagcggctac cacggcaaga ccatgggagc gctcagtctg accggcgcgg
     4501 ccaagtaccg ggaccccttc catccgctgc tgccatgcgt ccgccatgtc ccctacggtg
     4561 atgccgagtc actccaagcg gtgttggaga ccctgcccgg acgggcatgc ttcgtggtcg
     4621 agccggtcca gggtgaggct ggtgttgtcc tgccgccgga cggatatctg cgcagtgtgc
     4681 aggaactgtg ccgcgcctac gacgcgtgct ttgtgctgga cgagatccag accgggctgg
     4741 ggcggctggg ctcctggtgg ggcgccgacg ccgagggttt gaccccggac atcctgctca
     4801 gcggcaaagc gctcggcggc ggcgtcctac ccgtctccgc cgtgctcgcg agaccggagg
     4861 cgttcgccgc cttcgacaag gacccctaca tacacacctc gaccttctcc ggcctgcctc
     4921 tggccatggc cgccgtacgc ggcgtgctga cggccctgga ggaggaccat ctcgtggagc
     4981 gggcccgggt ccttggagac gagctgctgg cgcgcctgcg caccatcgcc caacaccacc
     5041 tgggcgacgg catcgtggtg gaggtacgcg gccgcggcct gctcatcggc atcgagttcg
     5101 ccatacccgg ccccgcggga gacctgctca tcgagctgat ctcccacggt gtcatcgcga
     5161 accactcact caattcctcc caggtgctgc gcttcacccc tccagccgtg ctgagccccg
     5221 ctgacatcca atacctgtgt gaagcgttcg accactcgtg tcgcgccctc gcacgccaat
     5281 acgcgccgat ctcaggaggt gccacccgct gtgcgtagcg tccacgtgct ggtcgacctg
     5341 ccgaacgtct cgccgtctgc gggatacgcg caggtgagcg acttcgcgcg ctatcccgag
     5401 ctggcctccg tggtccgcac tgtcaccgtc cacccccttg gggacggcca gcaaaagagt
     5461 gactgggaag tctatttccg caacggcatc ctgcgctgga ccgaagccga cgatttccac
     5521 ccccacgaac accgcatctc ctttcgccag accgacggtg acttcgactc cttcgccggc
     5581 acgtggcaca tcatcagcag tgataccgga tgccgagtgc gctttgcagc cgacttcgat
     5641 ttcggcatcc ctagcatggc agggatcctc gacccggtcg ccgaacgagt aatcaaggaa
     5701 accatcgccc gggtcgtgct gggactcttt gacaaccacg gcgcggtcat cggcgatccg
     5761 ctgctcgcca gagccgtcac agccactgcc ctgcctgtcc ccggccacgt ggcgcaggtg
     5821 ccctgatgga cgccaagaac ctgttcatga caccgaccac ggcggcactc gtgcgtgcgg
     5881 agtacgccct ggggctcctc gtcgccctga cactgttcct cagtcacctt cacgacgtcc
     5941 gctgggagcc ggctatcgga ctgttcgtgt acatcgacct gatcggctac ctccccggag
     6001 ccatccagat gcgccggagc tccacgggtg acctccacaa ggcctactac atcgcctaca
     6061 acgtcacgca cagcatgctc acccaggcac tggtcgccct gctgtggatg gcgttgctcg
     6121 gccccgaatg ggcactgctc accctcgcga ttcacctgtg cggcgacagg gccctgttcg
     6181 gtaatttcct caaacctttc ggcgtgcgct tcgaaccaca ccagcatccc gcataccacc
     6241 ggttccgccg cgaattttcg acggcccatc agaccagtca ccgccggccc gcgcgagacg
     6301 cctcatgacc ggcgcggcac accaccagtc ggcgtccggc agcgtaatga cccacgcact
     6361 gcagcgcttc gccgccggaa tcacggtcct caccttcgat gcccccggtg gagctgcggg
     6421 agtgacggtc agcaccctga cccccgtccc cggtacctgt cccatggtct cggtagcgct
     6481 gcgtcgggca agcaagtcac tcagcgagct gctgacggca gaggcgttca ccgccaatgc
     6541 cctggcggca gaccaagcga gtctggccca gcacttcgcg cgacgccgcc ggtccaggga
     6601 gacaagccag ctaccacccg acacatgggc ccaggcggct ggcatcccac gcctgaggca
     6661 ggccacttcc tggctggagt gccgggtgga ccagacggcg tccctcagag accacgtcct
     6721 ggtcatcgcg accgtgacgg caggcattcc cggcagcgga ccgcctctga tcggtttcac
     6781 cggtgcacta cacgctgacc tatccggcac caccacggca cgagggagtc acccatgacg
     6841 actgcagacg tccccgtcga ggcagactgg gaacgagcgc cttccctgat ggacggcgcc
     6901 atgagcgtcg aactaacggc cgcacaatgc gatctgaagt actggctcgc ctccgttccc
     6961 cacggcacgc tccagggtca cgtgatgggg catgcagacc acgtgcagcc gctagcgatg
     7021 atgcgcacac cgggtccgct caacaccgcg ctcaaagagg agctggccta ccggtctctc
     7081 gccgaagaga aagctacccg cgtcatcggc cacctcgtgg cactcgcccc cgacctggcg
     7141 tgcctggagt tctacgccac ccagctcctg gacgaggcac ggcatgcgat ggtgttccgc
     7201 agccatctgc tggacctggg cgtcagcgag gaaaggctgc atcaggagat ccaggaactg
     7261 gcgaagcagg acagcgacgc aatcctggat cccctggagg ccttcggact gcgcatcctg
     7321 cgcgacgaag gggacttcat cggcggtgtg ctggccctca ccgtgctggt tgaaggcgtg
     7381 ctggcccccg cagcagagct cagcgaacaa aagtggcgca tcttcgaccc agccgccgcg
     7441 gacatcgagc gaggagccgg catcgacgag atccgccacc tcaccgtggg cagctccatc
     7501 atccgtgagc acctcctggc ccacccccag gaaaagaagc gcctcctgcg actcatgcac
     7561 gagggtttcg ccctgtggga ctcgctgccg gttgatgacc aactgctcag acgtgaaaac
     7621 ctcttccagc aaggcatcga cgaacacagc catctcctcg gtgactacca ggtatggccc
     7681 ggccgccgac tggccgacac ctcggcacga gaacgcatca ctacaggaca gcagtgggca
     7741 cagcgcatgc aaagcgtccg gctggcgttc gcgggcctcg agggcacccc atgaacattc
     7801 ccgagatggc cgagccgccc gcacacagcc accgcccctc ccccacacag ccagcgaaca
     7861 tgaaggacat cacgcagctg gttaaagcgg cggggctcct ggaaccacag ccgctctact
     7921 acacgctcaa gatcttcacg aatctgggga tgctggcagc ggcctggacg gccttcgccc
     7981 tcctcggcga cagctggtgg cagctcgcca ccgccgcctg cctggccttc tgcttcgggc
     8041 agaccagcgt gatcgggcac gacgcggggc accgccagat cggccgtacc cgcaaggtgg
     8101 caaatctcgt cggctacctc cacgccaatg tactgacagg aacaaacttt cactggtgga
     8161 tcgggcacca cacgaagcac cacaacttcc ccaaccacct ctcgctcgac cccgacatcc
     8221 agcgccgcca ggtcatcttc gacccggcac accacgccag gaagaagggc accgtcaaac
     8281 gcttcatcat ccgccaccag gggtggatgt tctacgtgct catcccactc gaagggctac
     8341 gcctgaacct ggcaggctac atagccgcct accgatacgc cgcaatgagg aaagccacca
     8401 ccgacctgat cctgtgcacc acacatctcg tcgcctacct gaccctgctg ttcagcgcac
     8461 tcccactcgg tctggccgtt tccttcctgg ccgtgcacca gggcctcttc ggcgtgtacg
     8521 tgggcctgct gttcgcaccg aaccacaaag ggatgccgct ccgcgacggg cagcaggaag
     8581 aacaggactg gctgccgcgc caggtcctca gctcccgcaa cctcgccccc aaccggctca
     8641 cggacttctt ctatgccggg ctgaactacc agatcgagca ccatctgttc cccacgatgc
     8701 cccgcaggag cctgcacaaa gctcaacccc tcgtgaaacg gtacgtcctt acccacggtc
     8761 tgccttacac ggagacctcg ccgctgcgct cctaccgcga catcacaagg ttcctcacca
     8821 tggtcagcca ccaggtacca gaacgcgcgg attccctcac gaaggctcgt ggaggcgctc
     8881 aatgaggcaa gcagcagtcc tgagtgggct gggcagttgg ctgccgcccc aggtcgtcac
     8941 caacgaccgt ctcgcccgcc aactggacac ctcggatgag tggatacgcc gccgaaccgg
     9001 aatcggacag cgccacatcg ccgggcccgg catcgccacg tcagacctgg ccatcgaggc
     9061 cggccgacgg gcgctgaagt ccgcagcgac cgaccgggcg gactgcgtca tcgtggccac
     9121 cacgacaccg gatcacccct gcccggccac cgctcctatg gttgccgcga aactggggct
     9181 gggaaccgtg cccgccttcg acgtggcagc ggtgtgcgcg ggcttcctgt atgcgggggc
     9241 cgtcgctgag ggcctcatcg ccgcgggaac ggtgcgcacc gcgctcgtca ttggggcgga
     9301 cacgttctcc agcattctcg accctgaaga ccgcacgacg cgttgcgtct tcggtgacgg
     9361 agccggtgct gcgtacctgc gcgccggctc gccggaggaa cccggtgccc tcggcccgtt
     9421 cacgctgggg agcgacggaa caggcgctga catgatcatg gtgggcggag ggggttcacg
     9481 ccagccgctc cgggcagaga acatcgccag gtccgacccc tacttccgta tgcagggcaa
     9541 agccgtcttc cgccacgctg tcgagcgcat gagcacagcc atctcaaccg ccatcgaggg
     9601 cgccaactgg actcttgacg acgtcgaggt attcgtcact caccaagcca acttgcgcat
     9661 catgcaagcg gtagccagcc acctcggcgt ccccccagaa cgcttcccgg tccacctgga
     9721 ccgcgtgggc aacacagccg ccgcgtccgt gcctctgacc ctggtcgacg ccgcctcagc
     9781 cggcaccctg cgagcgggcc agcgcacggt cttagaggtc ctaacagaag tggttgatgg
     9841 tgtgactgtt ggcttgtccg ctcgttgg
//
