LOCUS       NZ_BMVR01000023        10756 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces flavofungini strain JCM 4753 sequence23, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVR01000023
VERSION     NZ_BMVR01000023.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces flavofungini
  ORGANISM  Streptomyces flavofungini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 96504)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650815.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:46:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,873
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,638
            CDSs (with protein)               :: 7,638
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 43 of 161
            Pseudo Genes (incomplete)         :: 137 of 161
            Pseudo Genes (internal stop)      :: 10 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 67381
            Orig. end                         :: >78137
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVR01000023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..>10756
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..>10756
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..>10756
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:>5756](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..>5756
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(806..824)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.9"
                     /tool="antismash"
     misc_feature    complement(1084..1096)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     gene            1889..2812
                     /locus_tag="IE274_RS37095"
                     /old_locus_tag="GCM10010349_75680"
     CDS             1889..2812
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE274_RS37095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010349_75680"
                     /product="hypothetical protein"
                     /protein_id="WP_190120347.1"
                     /transl_table=11
                     /translation="MPPYPHRSSLLLLPFADDPGRLERAEHRARRQPFRTQIHTDERTV
                     WGWLRWALPSDSRSGEDTCPLLEIGIRPPEPSLRDRCAHRWLTRRPAARVALDRGGTVT
                     LAIALVSLIGLVLALGRDAPGAVAIPLTLLLPLLVDHLPARLDARARRRVRTVTDPASV
                     TYLRPLVAWHAQIDHTARHHPTPEQLDAARLSHRALWDLAGLLSQPTSPPHDHRDLLIR
                     AHLLADLAYQSVEHATAHHQLEDAITCPPKPHGDRQGSGVEGPPAGNEPTAPTAELALG
                     AEALHDAAAAARYATGCLHRLDKSRT"
     gene            2845..3402
                     /locus_tag="IE274_RS37100"
                     /old_locus_tag="GCM10010349_75690"
     CDS             2845..3402
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006136004.1"
                     /locus_tag="IE274_RS37100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_75690"
                     /product="hypothetical protein"
                     /protein_id="WP_190120348.1"
                     /transl_table=11
                     /translation="MHTYQPPTPTTPRLYLLFVHEPYWPPQGTTEVNATIVAAATLLHP
                     RVLQPDGLRIHDLLSSGQRKRGEIVPMATLTAELGDTGWRNVAAWERVAVDLLTLSRFG
                     DCHALSVALPAVDRALLCNGPDVTINSFDSASGNCEVHGPADRESLLRTLTEWLRQAEA
                     ERPLWPGDTLLPKPHPGTQDEA"
     misc_feature    3292..3294
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3609..4250)
                     /locus_tag="IE274_RS37105"
                     /old_locus_tag="GCM10010349_75700"
     CDS             complement(3609..4250)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037514.5"
                     /locus_tag="IE274_RS37105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_75700"
                     /product="hypothetical protein"
                     /protein_id="WP_229910064.1"
                     /transl_table=11
                     /translation="MDEQEDDLGREHLAFASGMTMEEVVRSTVRASGDAMMAQVRREAM
                     AARLRVRGHGGTITAMDRMWMRQAIDLAGRCPPSDGAFSVGAVLVDASGQKLACGYSRE
                     DDPHVHAEESALGKLNPADARLTGSALYTTLEPCSQRKSRPVTCTEHILRTQISRVIIA
                     WREPSLLVADCVGVETLRADGVIVDELPDWADAAHAVNAHLLGLPQSDKT"
     misc_feature    complement(4323..4337)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 23.07"
                     /tool="antismash"
     gene            4565..4963
                     /locus_tag="IE274_RS37110"
                     /old_locus_tag="GCM10010349_75710"
     CDS             4565..4963
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495434.1"
                     /locus_tag="IE274_RS37110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_75710"
                     /product="DUF5958 family protein"
                     /protein_id="WP_190120349.1"
                     /transl_table=11
                     /translation="MNERDFRLNELVQGLRPMPEGVEWFDGLDSAEQSEVLLFLRHHCV
                     QARAVAEDAEESIRRAGLRPTHTPAVLISQGRIDQQLGKIAGLTPLDERRKAFRLLIAV
                     LTVADKRRRERFCSGGCGHWWHSLSAAG"
     gene            5001..>5756
                     /locus_tag="IE274_RS40500"
                     /pseudo=""
     CDS             5001..>5756
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /locus_tag="IE274_RS40500"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /pseudo=""
                     /sec_met_domain="YcaO (E-value: 1.1e-45, bitscore: 149.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNYGWALDEVRLEWTYGTGLTSGRKVEVPVSVVRRQAQPTLWEP
                     DLFRVTSTGLACGNTRDEARLHAMYEVVERDALFADETVHGTYRTLIDPATVDDLYCLR
                     LLGQLREAGVSVELAVVDNSFALPVCLACLWSPDYPATFAGAGCHLQPCIALARAITEA
                     AQSRLTCIARTRDDLPSHNGLFDTAPPRPDGTSQRPYALWNLWPTRRGLPADLAGQARH
                     VARRITHITGHEPVALDLSEPADPVAAVKV"
     gene            5800..6636
                     /locus_tag="IE274_RS37120"
                     /old_locus_tag="GCM10010349_75720"
     CDS             5800..6636
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477227.1"
                     /locus_tag="IE274_RS37120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_75720"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_229910065.1"
                     /transl_table=11
                     /translation="MITNQYDDIRHLQDAFDAVHTARAASRLVDGLYAQAMGDAYPVEV
                     APTSSCDWPLLGTLTTNLRLGPGDVLADVGCGTGGVGLWLARALAVRLVGVDISPVAVR
                     LAATRAATFVPQGQAQFHTGTLTATGLSDRHVNAVICVDALSRADDRQGALRELYRILR
                     PGGRAVITRATRHGARGPLTVHEEAAGFAVERVDVRAAEPHMWGRLYGLWLSRENELRR
                     ELGNREAERMLAEARRQLPRLPDRTARVITLQRPADGRPASAGTLPETAVYREESG"
     gene            6681..7639
                     /locus_tag="IE274_RS37125"
                     /old_locus_tag="GCM10010349_75730"
                     /pseudo=""
     CDS             6681..7639
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /GO_function="GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /GO_process="GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675229.1"
                     /locus_tag="IE274_RS37125"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_75730"
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MVLADAGNDVLIHARRKEIADAINTRHENPATICPAPPCYSRYGR
                     QPTRPSRSPRPTSQSCPSSRRHCARTSPRGRRSSAPTPWS"
     gene            complement(7727..10330)
                     /locus_tag="IE274_RS37130"
                     /old_locus_tag="GCM10010349_75740"
     CDS             complement(7727..10330)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019074628.1"
                     /locus_tag="IE274_RS37130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_75740"
                     /product="ATP/GTP-binding protein"
                     /protein_id="WP_190120350.1"
                     /transl_table=11
                     /translation="MTSAVSLLTSQDEQVAGALTGLVKALLWLVTHWWVLALAVCGVWG
                     AGVSVGRRLARRASAERMALELVPSRHFDPSLEEIFRRGVQLARASTSMPWWAPRCTRT
                     VQVRLRSDGSTPLCYRIEGPAGAQRLLSITPFGPNVTVTRARPITDEPRRHTVRAEFIL
                     RGSPVSPLREVPLDPDPLQPLADAVSDLRAELGDLAEVRLDVQRAPKWALRLRRIHLMG
                     AARRSEHRETQRAARLVRKDATRIEDSFSWHLQQLVSGSKTGGGGRRLVMPPVPRRVDR
                     SEALGKLAEDDHLVRVQLLVLCASNVQGRAEARLAQLQAALDVFGGRARWSMLGWRIGP
                     WRLGADHWPSRGHFDRRWELGHCAPPRPNWVRLEEIAGLLKPPTVHCRVPLLGGDLPTF
                     SFGDPDLLLQGIYRGADGRERLVATYAEETLFEVGVGKAGGGKTERALAQAIGWAHAGG
                     GLMFIDPHRDSWPRAMTFLGHDHVMRRLALIDLTTTGSASRLGSWNPLGMHQGQVAHEV
                     VETATDAYASAMGWDNLNAPRAITILTASLAVLVALNRAACRAGRPEDQATLLHVRPLI
                     TDGVFRSSALTALARELDEETLAWWRTTFPTLPSDAFAVVLNGLGRLAMNPVTRAFLGQ
                     PVGRYNIRAAMDHRMIVWVCTSGNGPTDRLLTALLAHDLLRAGRSRVDTPEDQRVAFRA
                     YFDELITLTGSAPETIAAMFEDFRKYRVQVHGMTQLLGRLPAPVRHSLVQNASTLATAA
                     GSKTAIAPVTAEWGDNPSADQVATLDRFEHYASLTVRGRRVGPVPLRGPHLDGVFAAQA
                     RPRQIPDVERAAHATAGALPLDQLTERADAQLERVSAFLAQRVQTNTGTASMRYE"
     misc_feature    complement(10347..10360)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.13"
                     /tool="antismash"
ORIGIN
        1 caccgcgagg cggcccagcc gcacccggcg gtgcatctgg ctgagcgccg caccggcggc
       61 caccggcggc cccgccgggt ccggtgatgc gtgggaagaa ggcatgaggt ggtctcccgg
      121 agagagcgtg gaggctgata ccgagagaca acccagcggc cccgaccggc tgaccgacca
      181 gcgcgccccg accacagcga cgaatcggtc acgaagaagc ggtcacggca gcggtcgcgc
      241 taccaccgcc tcgaccaccg gcgcggcgac gatgaccggt gcgaggccag caccgcgcga
      301 gcaccgaccg ccgcctcgtg accgatggcg cgcggctctc tcctcgctgc gcgggcgggg
      361 ttcgtcaggc agcccacaac tgcgcccgcc tgacgcgggg cggagcgggt acgcgtcgac
      421 ggccccggca ggcgggctcg gcaactcctg ccccagtggc agtgacctgc gtggtcgggg
      481 caccgtcagc cggtggtggt cgggttctac caccacaccg gtccgcgccc gcagcgtgat
      541 cccgaaggtc tcccacgccc tggggaatcg gtgaccggaa cggccacgcg cccacgcggg
      601 aaccgggcgc gggcatcggt caccactcac accccgaggg gatgaccggc aggacacagc
      661 ggtcgtgacc ggtgaccgat actgcggaat cagtcaccgg tcacccaccc gcacggccat
      721 cggtcacggc cacccctcac cccgtgcacg gtgcgccgcg cgtccgggcg acggcagagg
      781 acggcgacca ccgtgcccgc cgcagcttgt gattccttcg taatcctggt cgggggccgg
      841 tggtcgccag ggtggtcggg gccggcgggt tgcctgacat gcatgaacca cgctcctcgt
      901 gccgcggccg ccccgccccg agtccgcgtc gccccgatgc cctgcgcggc acccgcagct
      961 cgcgctgccc gcgcgccgga acccaccggc agcagccgca cgcccgggcg tgctcgggtg
     1021 gcgcggggcg ggacgccacg gtgagcggct acggatcggg gatccgccgc gggcagatgg
     1081 cgggtgacca cttcacccag atcgccaacg ccctcttccg cgaccggcgg atcagcttcc
     1141 gcgccaaggg gatcttcgga ctgatctcca cccaccggga cggctggaag gtcagcgtcg
     1201 ccgagctcgc gcgcgccggt cccgagggcc gcgacgcggt caccagcggc ctcgaggaac
     1261 tggagaagta cggctacctg acccgcggcc gcgaacggcg cgccgacggc acgctgggcg
     1321 ccgccgtcta ctccatcacc gacatgcccg cacacctcta cgacctgctc ggcgaccagg
     1381 cacccacacc cctcaccgag gacgggaaag tccgcaggtc atcaccagag tcggaaaagc
     1441 ctgcgctggc tcaacctgcg caggcccgac gccccacaaa gaacaccaca gtcaagaaga
     1501 ccacccagca gaacaccacc cccgtccatc cctccgcccc cgacgcggcc gtgaacgcgc
     1561 gcgacgaccg cccctccacc gggcccgccc cgcagccgcc gcagcccggc cctcgcatca
     1621 aggctacccc cggcgtacgg ctgctcctgg ccatcggagc cgagcacccc catctcctgc
     1681 tgaccggccc cgccctccag gagcaaggag cggtggccac ccagctcctc accgaaggat
     1741 ggacgcccga actcctgcgc cacgtcatcg ccgaccgccc gctgcccacc ccgctgcgct
     1801 cgacggtcgg cgccatcatc gccacggcac cacccggccg caccacccgg cgaccggctg
     1861 agtgtccact caccgaccga gggagaccat gcccccctac ccacaccgca gcagccttct
     1921 cctgctcccc ttcgccgacg acccgggccg cctggagcgc gcggagcacc gcgcccgccg
     1981 ccagcccttc cgtacccaga tccacaccga cgagcgcacc gtctggggat ggctgcggtg
     2041 ggcacttccc agtgactccc gctcgggcga ggacacgtgc ccactgctgg agatcggcat
     2101 caggccgccc gagccctcgc tacgcgaccg gtgcgcacac cggtggctca cacgacgccc
     2161 cgcggcacgc gtcgcactcg accgcggcgg caccgtcacc ctcgccatcg ccctcgtctc
     2221 cctcatcggc ctggtcctcg ccctcggcag ggacgccccc ggcgccgtcg ccatcccgct
     2281 gacgctgctg ctgccgctcc tcgtcgacca cctgcccgca cgcctcgatg cccgtgcccg
     2341 gcgccgcgtg cgcaccgtca cggaccccgc ctccgtcacc tacctgcgac ccctcgtcgc
     2401 ctggcacgca cagatcgacc acacggcccg gcaccacccc acacccgaac agctcgacgc
     2461 cgcccgcctc agccaccgag ccctgtggga cctcgccgga ctgctcagcc agcccaccag
     2521 cccaccccac gatcaccggg acctgctcat ccgcgctcac ctgctcgccg acctcgccta
     2581 ccagtccgtc gaacacgcca ccgcccacca ccagctcgag gacgccatca cctgcccgcc
     2641 caaaccccac ggcgaccgac agggcagcgg cgtcgagggc cctccggccg gaaacgagcc
     2701 gacggcgccc accgccgaac tcgccctcgg cgctgaagcc ctgcacgacg ccgcggccgc
     2761 cgcacgctac gccaccggat gcctgcaccg tctcgacaag tcccgcactt gacccacgaa
     2821 cgcgacgcga aaggaccgac cgccatgcac acctaccagc cgcccacgcc cacaacgccc
     2881 cgcctgtacc tgctcttcgt ccacgagccg tactggccgc cgcagggcac taccgaggtc
     2941 aacgccacca tcgtcgcggc cgccacgctc ctgcaccccc gcgtcctgca gcccgacgga
     3001 ctgcgcatcc acgacctcct ctcgagcgga cagcgcaaac gcggcgagat cgtgccgatg
     3061 gccacgctca ccgccgaact cggcgacacc ggatggagga acgtcgcagc ctgggagcgg
     3121 gtcgccgtag acctgctcac cctgtcgcgc ttcggagact gccacgcgct ctccgtcgcc
     3181 ctgcccgccg tggaccgcgc cctgctgtgc aacggccccg acgtcacgat caacagcttc
     3241 gactcggcct ccggcaactg cgaggttcat ggacccgccg accgtgaatc cttactgcgc
     3301 accctcaccg aatggctgcg ccaggccgag gcggagcggc cgctgtggcc cggcgacacg
     3361 ctcctgccca agcctcaccc cggcacccag gacgaggcat agcctgctga agtatccgac
     3421 cgcatcgacg ggttggcgct tcgagcggct ctttccgaga atcgccgggg cgccccccgc
     3481 aaggggttcg tcgaaagcgc cgtcgggctc tgactgaggc cgcccgtgcg ggaacttgcg
     3541 aaagcttcgg cgccatctca cggcgcgagc ccggccgcgc tacgcggggc gtgtgacgtc
     3601 gcggatcatc acgtcttatc ggactgtggg agtccgagga gatgagcgtt gacggcgtgg
     3661 gcggcgtcgg cccaatccgg gagctcatcg acgatgacgc cgtcggcgcg gagcgtctcc
     3721 acaccgacgc agtcggcgac gagcaggctg ggctcgcgcc aggcgatgat gacgcggctg
     3781 atctgtgtgc gaaggatgtg ctcggtacag gtgaccggtc tcgacttgcg ctgggaacag
     3841 ggttccaacg tggtgtagag ggcggagccg gtgagcctgg cgtcggccgg gttgagcttg
     3901 ccgagggcgc tctcctcggc atggacgtgc gggtcgtctt cccgcgagta gccgcatgcc
     3961 agcttctggc cgctggcgtc cacgagcacg gcgcctacag agaaggcgcc gtcggagggt
     4021 gggcagcggc ctgcgaggtc gatggcctgc cgcatccaca tgcggtccat ggcagtgata
     4081 gtgccgccgt gtccccgcac gcgcagccgg gcggccatcg cctcccggcg cacctgggcc
     4141 atcatggcat cgccgctggc tctgacggtg gatcggacca cctcctccat cgtcatgccc
     4201 gacgcgaagg cgaggtgctc cctgcccagg tcgtcttcct gctcatccat cgtgcccctc
     4261 ccacggcgcc aggtgcccca gggcgcacgc tacggggccc accccgtccg tggcagcccg
     4321 gagtgaaagc gctcccgacc acagggcgac agctacctca aggccagagg ttcccgcaca
     4381 ggcccgcctg actggagata gcttccagtg ccgtgccgat gcctctcgca atgagttggt
     4441 gttcgagcag ctgcgcaagg cagagtggcg tggtcagtga gctgtgggta gaagccctga
     4501 actgggtgga tgtgaatccg ctccagcacc acggccgagc gccgcctgat ctcacggggc
     4561 agtcatgaac gaacgcgact tccgactcaa tgaactcgtt cagggcctgc gcccgatgcc
     4621 cgagggggtc gaatggttcg acggcctcga ctcggcagaa cagtccgagg tcctgctgtt
     4681 cctgcgccat cattgcgtcc aggcgcgcgc tgtcgccgag gatgccgagg agagtatccg
     4741 ccgtgccggg ctgcgcccaa cgcacacgcc ggcagttctg atctcacagg gccggatcga
     4801 ccagcagctg ggaaagatcg ctggtctcac acctctcgac gagcgccgga aggcgttcag
     4861 gctactgatc gcggtgctca cggtcgccga caaacggcgc cgcgagcgct tctgttccgg
     4921 tggctgcggt cactggtggc acagcctgtc cgccgctggt taggaccgtc gactgcctcg
     4981 ccgcggcgct gccgttgaag gtgtccaact acgggtgggc cctcgatgag gtccgcctgg
     5041 agtggacgta cgggaccggg ctgaccagcg ggcgcaaggt cgaggtgccg gtcagcgtcg
     5101 tgcgccgcca ggcacagccc accttgtggg agccggacct gttccgggtg accagcaccg
     5161 gcctggcctg tggcaacacc cgcgacgagg cacgtctgca cgccatgtac gaagtcgtcg
     5221 aacgagacgc cttgttcgcc gatgagaccg tgcacggcac ctaccgcacg ctcatcgatc
     5281 cggccacggt cgacgacctg tactgcctgc gcctgctcgg ccagctgcgg gaggcgggtg
     5341 tgagcgtcga gctcgcggtc gtcgacaact ccttcgccct gccggtctgt ctcgcctgcc
     5401 tgtggtcgcc cgactatccc gccacgttcg ccggagccgg ctgccacctg cagccgtgca
     5461 tcgcgcttgc acgcgcgatc accgaggcag cgcagtcccg cctgacctgc atcgcccgga
     5521 cccgcgacga cctgccctcc cacaacggcc tcttcgacac cgccccgccc cgcccggacg
     5581 gcacatcgca gagaccgtac gccctgtgga acctgtggcc cacccgccgg ggccttcccg
     5641 cagacctcgc gggccaggcc aggcacgtcg cccgccgcat cacccacatc accgggcacg
     5701 aaccagtggc cctcgacctc tccgaacccg ctgacccggt cgcggctgtc aaggtcatct
     5761 gcccgggacg cgctcacgac gacgccgggc ggtactccgg tgatcaccaa ccagtacgac
     5821 gacatccgtc acctgcagga cgctttcgac gccgtgcaca ccgcacgggc cgcctcccga
     5881 ctcgtcgacg gcctctacgc ccaggcgatg ggcgacgcct atccggtcga ggtcgcgccc
     5941 accagctcct gcgactggcc cctgctcggc accctcacca ccaacctccg gctcggcccg
     6001 ggcgacgtac tcgccgacgt cggctgcggc accggtggtg tcggactctg gctcgcgcga
     6061 gccctggccg ttcgcctggt cggcgtcgac atctcaccgg tcgccgtccg cctcgctgcc
     6121 acgcgggcag ccaccttcgt cccacaaggc caagcccagt tccatacggg cacactcacc
     6181 gcgacaggcc tgtccgaccg gcacgtcaac gccgtcatct gcgtcgacgc gctatcccgc
     6241 gccgacgacc gccaaggcgc cctgcgtgag ctgtaccgca tcctgcgccc cggcggtcgc
     6301 gccgtcatca cccgcgccac acgccacggc gcccgcggtc cgctcaccgt ccacgaggag
     6361 gctgcggggt tcgccgtcga acgcgtggac gtacgcgcgg ccgagccgca catgtggggc
     6421 cgcctgtacg ggctgtggct ctcccgcgag aacgagctac ggcgcgaact cggcaaccgg
     6481 gaagccgagc gcatgctcgc cgaggcacgc cgccaactgc cccgcctccc cgaccgcacc
     6541 gcccgcgtga tcaccctgca acgaccagca gacggccgac ccgcgtcggc cggtaccctc
     6601 cccgaaaccg ctgtgtacag ggaggaatca gggtgaggac tgcagtcctg tcggccggat
     6661 catggggcac cacctacgcc atggtgctcg cggacgccgg gaacgacgtc ctcatccacg
     6721 cccgccggaa ggagatcgcg gacgcgatca acacccgcca cgagaacccc gcgactatct
     6781 gcccggcacc accctgctac tcacggtacg ggcgacaacc gacccggccg tcgcgctcgc
     6841 cgaggccgac atcgcagtcc tgtccatcct cgcgcagaca ctgcgcgcga acctcgccgc
     6901 gtgggcgccg ttcatcggcg ccgacaccgt ggtcgtgagt ctcatgaagg gcatcgagac
     6961 cgacaccggg ctgcgcatga gcgaggtcgt cgcacaggtc gccgatctac ccacggaccg
     7021 cgtcgcggtg ctctccgggc ccaacctggc ccgcgagatc gccgcccgcc agcccgcggc
     7081 ttcggtcatc gcctgcgtgg acgagaaggt cgccgagcga ctccagaagg cctccccgac
     7141 ctcgtacttc cggccctaca ccaaccctga cgtcgtgggc tgcgagctcg gcggcgcggt
     7201 gaaaaacgtc atcgcactcg ctgtcggact ttccagcggg ctgggactgg gcgacaacgc
     7261 caccgccctc ctcatgaccc gaggactggc ggagaccgca cgcctgggac aggcgctcgg
     7321 tgcagacccg atcaccgtct ccggcctggc cggcatcggc gacctggtcg ctacctgctc
     7381 ttccccgctc tcccgcaacc gtaccttcgg cgaacacctg ggcaagggca tgaccgtccg
     7441 tgaggcagcc atcgccacgt cacagactgc cgagggtgtg aagtcagccg cagcccttct
     7501 cgcggtgacc cgccggcacg gcgtggaaat gccgatcact gagatcgtgg tcgatgtcat
     7561 cgacggccgg ttgaccgcgc gtgaggcagc cgatgtgctg atggcccgca cccccaaggg
     7621 cgagcgatac ggaaactgat caccgattga gcctgtcggg ttgtcgacct cagccttctt
     7681 cgacctggcg tagccgtgta cggatagcgc gtccgcgcac cgggcttcat tcgtatctca
     7741 tcgacgcggt cccggtgttg gtctggacgc gctgggcgag gaaggcgctg acgcgttcga
     7801 gctgggcgtc cgcccgttcg gtgagctggt caaggggcag cgctccggcc gtggcgtggg
     7861 cggcccgctc gacgtcgggg atctgacgag ggcgggcctg ggcggcgaag acgccatcga
     7921 ggtgagggcc gcgcaggggc accggtccga cacggcgccc acggacggtc agtgaggcgt
     7981 agtgctcgaa cctgtcgagg gtggcgacct ggtccgcgct ggggttgtcc ccccactccg
     8041 cggtaacggg ggctatcgcc gtcttcgagc ctgcggcggt ggccagtgtg gaggcgttct
     8101 ggaccaggga atgccgaacg ggtgcgggca ggcgtcccag gagctgggtc atgccgtgga
     8161 cctggacccg gtacttgcgg aagtcctcga acatggcggc gatcgtctcg ggggccgagc
     8221 cggtcagcgt gatcagctcg tcgaagtagg cacggaaggc aacccgctgg tcctcggggg
     8281 tgtcgacccg ggaacgccct gcccgcaaca ggtcgtgggc gagaagggcg gtgagcagac
     8341 ggtcggtggg cccgttgccg gacgtgcaca cccacacgat catccggtgg tccatcgccg
     8401 cgcggatgtt gtagcggccg accggctggc cgaggaacgc tctggtcacc gggttcatgg
     8461 ccaggcggcc gagcccgttg agaacgacgg cgaaggcgtc ggagggcagc gtcgggaacg
     8521 tggtgcgcca ccacgcgagc gtctcctcat cgagctcacg agccagggcc gtgagcgccg
     8581 agctgcgaaa gacaccgtcg gtgatgagtg gcctgacgtg gaggagcgtg gcctggtcct
     8641 cgggccgccc ggcccgacag gccgcccggt tcagggccac cagcaccgcc agtgaggcgg
     8701 tcaggatggt gatcgcgcga ggggcgttca ggttgtccca gcccatcgcg gaggcgtagg
     8761 cgtcggtggc cgtctccacg acctcgtgag cgacctgacc ctggtgcatg cccagcggat
     8821 tccacgaccc cagccgggac gccgaacctg tggtggtgag atcaatcagc gcgagccgcc
     8881 gcatcacgtg gtcgtggccg aggaaggtca tggcgcgcgg ccaggagtcg cggtgcgggt
     8941 cgatgaacat caacccgccg cccgcgtgcg cccagccgat cgcctgggcg agcgcccgct
     9001 ccgtcttgcc gccgccggcc ttgcccacac cgacctcgaa cagggtctcc tcggcgtagg
     9061 tggccaccag gcgctcgcgg ccgtcagcac cccggtagat cccctggagc agcaagtccg
     9121 ggtcgccgaa ggagaaggtc ggcaggtcac cgccgagcag cggcacacgg cagtgcacgg
     9181 tcggaggctt gagcaatccg gcaatttcct ccaggcgcac ccagttcggc cgcggaggag
     9241 cgcagtggcc caactcccat ctgcggtcga aatggccgcg gctgggccag tgatcggctc
     9301 ccagccgcca cggaccgatc cgccacccga gcatcgacca ccgtgcgcgg ccgccgaaca
     9361 cgtccagcgc cgcctgtagc tgagccagcc gcgcctcggc gcgcccctgg acgttcgaag
     9421 cacacagcac cagcagctgc acccgcacca ggtggtcgtc ctcggcgagc ttgcccaacg
     9481 cctccgaccg gtccaccctg cgcggcaccg gcggcatgac cagccgccgc ccgccacccc
     9541 ccgtcttgct gccgctgacc aactgctgca gatgccagga gaaggagtcc tcgatccggg
     9601 tggcgtcctt tcggaccagc cgggccgcgc gctgtgtctc ccggtgctcc gagcgccgtg
     9661 cggctcccat caggtggata cggcggagcc gcagcgccca cttgggcgcc cgctgaacgt
     9721 cgagacggac ctcggcgagg tcgccgagct cggcacgcag gtcagacacc gcgtcggcca
     9781 agggctgcag cggatcaggg tccaagggaa cctcccgcag cggcgatacc gggctgcccc
     9841 gcaggatgaa ctccgctctg accgtgtgcc tgcgcggctc gtccgtgatc ggccgcgccc
     9901 gggtgaccgt tacgttcggc ccgaagggcg tgatggacag cagccgctgt gccccggcgg
     9961 gcccttcgat ccggtagcac agcggggtgg agccatccga gcgcaggcgc acctgcacgg
    10021 tcctcgtaca gcgcggagcc caccagggca tgctcgtcga ggcgcgcgcg agctgaacac
    10081 cgcggcggaa gatctcctcc aggctcggat cgaagtgccg ggagggcacg agctcgagcg
    10141 ccatccgctc agccgaagcc cgccgcgcga gccgacggcc caccgacaca ccagcccccc
    10201 agaccccgca gacggccagg gcgagcaccc accagtgcgt gactagccac aacagggcct
    10261 tgaccagccc ggtcagggcg ccggccacct gctcgtcctg gctggtcagg agcgacaccg
    10321 cggaggtcaa gccagcaccc cgatcgtgaa cggatgttcc ggacgctggg gcgtcaccag
    10381 gcctctgcgc agaggccgca caagcgcggt cacaggacgg tccacggccg gggttcgccc
    10441 ccggtcaaag ggacccacac ggccagattc ccgccgttgt gctcgatctc ctccaacacc
    10501 gcagccccga gcgggacgct gcgtgcgagg gccgcgacga gggggtgctg agccaccatg
    10561 gcctgaagat cgctgatccg gttcgccagt ctggtcggag aggcagctgt gagcacgaac
    10621 agcacccgag ggaagaccgg ataccagcgc agccatgcag ggcccatgga ggaagtgcgt
    10681 cgacgcccgc ccggcggagc ctcgtacagg tacagccggg cgtactcgat cagcttcacg
    10741 gccagccgct cgctgg
//
