LOCUS       NZ_BMVR01000006        10402 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces flavofungini strain JCM 4753 sequence06, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVR01000006
VERSION     NZ_BMVR01000006.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces flavofungini
  ORGANISM  Streptomyces flavofungini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 649308)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650815.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:46:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,873
            CDSs (total)                      :: 7,799
            Genes (coding)                    :: 7,638
            CDSs (with protein)               :: 7,638
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 43 of 161
            Pseudo Genes (incomplete)         :: 137 of 161
            Pseudo Genes (internal stop)      :: 10 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 75324
            Orig. end                         :: 85726
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVR01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10402
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10402
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10402
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5402](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5402
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(180..1268)
                     /locus_tag="IE274_RS16815"
                     /old_locus_tag="GCM10010349_34370"
     CDS             complement(180..1268)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989359.1"
                     /locus_tag="IE274_RS16815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34370"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_190116598.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 5.4e-16, bitscore:
                     53.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSERAVLHVKGRVLVGPEDVRDELWVVGGRVTYRRPAAGDVETVS
                     GWALPGLVDAHCHVGLDAHGPVAPDVAEKQARTDRDAGTLLLRDAGSPSDTRWIDDRED
                     LPKIIRAGRHIARTRRYIRNYAHEIEPDDLVAYVAREARRGDGWVKLVGDWIDRDAGDL
                     TACWPRGAVEAAIAEAHRLGARVTAHCFAEDSLPDLVEAGIDCVEHATGLTEDTIPLFA
                     ARGVAIVPTLVNIATFPQLADGGEAKFPRWSAHMRRLHARRYDTVRAAYDAGVPVYAGT
                     DAGGSLAHGLIAGEVAELTKAGIPPVDALSAAVWRARDWLGRPGLVEGAPADLVVYGED
                     PRADMRVLAAPSAVVVNGRVVG"
     gene            1412..2512
                     /locus_tag="IE274_RS16820"
                     /old_locus_tag="GCM10010349_34380"
     CDS             1412..2512
                     /NRPS_PKS="Domain: Aminotran_5 (51-295). E-value: 2e-26.
                     Score: 84.7. Matches aSDomain:
                     nrpspksdomains_IE274_RS16820_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644018.1"
                     /locus_tag="IE274_RS16820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34380"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_190116599.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.7e-21, bitscore:
                     71.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLGAAHFAAIEDRVARLLDTEQDVVIMQGEALLPLE
                     GCIRGAARPGTKALNVITGPYGQTFGNWLRDCGATVVDLAVPFHTAVTAAQVEEAFAAH
                     PDIDFVSLVHAEAATGNTNPVAGIGEVVRAHGALFMLDAVASVAAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWQRMAANPQAPRRSYLSLLDWKERWIDGGRKALLHAP
                     AQLEMLALEACVERIEAEGLTALMGRHATAATATRAGALALGGGLEPYVYEAGDAAPVA
                     TTLRAPRGVDAADLVARALAADPVLPLVAGGGALAKEMIRVNHYGPDATRGVVHASLAA
                     LGAAMGEVGLGVDLEAARRAVTDTWA"
     aSDomain        1565..2296
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS16820_Aminotran_5.1"
                     /evalue="2.00E-26"
                     /label="IE274_RS16820_Aminotran_5.1"
                     /locus_tag="IE274_RS16820"
                     /protein_end="295"
                     /protein_start="51"
                     /score="84.7"
                     /tool="antismash"
                     /translation="ARPGTKALNVITGPYGQTFGNWLRDCGATVVDLAVPFHTAVTAAQ
                     VEEAFAAHPDIDFVSLVHAEAATGNTNPVAGIGEVVRAHGALFMLDAVASVAAEPLLPD
                     AWGVDLCVIGAQKAMGGPAGVSAVSVSERAWQRMAANPQAPRRSYLSLLDWKERWIDGG
                     RKALLHAPAQLEMLALEACVERIEAEGLTALMGRHATAATATRAGALALGGGLEPYVYE
                     AGDAAPVATTLRAPRGVDAADL"
     misc_feature    2795..2810
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2944..2958
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.38"
                     /tool="antismash"
     gene            2967..3524
                     /gene="ectA"
                     /locus_tag="IE274_RS16825"
                     /old_locus_tag="GCM10010349_34390"
     CDS             2967..3524
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004932958.1"
                     /locus_tag="IE274_RS16825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34390"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_190116600.1"
                     /transl_table=11
                     /translation="MTAAQADLPTDLQAKFLEMPEGLRLDTPEVADGAAIWRIARDSKV
                     LDLNSSYSYLLWCRDFAATSVVARDTGGETVGFITGYIRPDKPRTLVVWQVAVDHAQRG
                     RGLAGALLDGLAAKVADRHGFDALETTITPGNTASERLFTSFAERHGARVERTVLFDAA
                     LFPGDDGGHEPEVLYDIAPLFR"
     gene            3633..4904
                     /gene="ectB"
                     /locus_tag="IE274_RS16830"
                     /old_locus_tag="GCM10010349_34400"
     CDS             3633..4904
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 7e-76.
                     Score: 247.3. Matches aSDomain:
                     nrpspksdomains_IE274_RS16830_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="IE274_RS16830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34400"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_190116601.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.3e-81, bitscore:
                     267.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMFDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNLILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDHYLDDSMPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRSLADLCRRQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGMPMA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTAAATLETYWADGSAMEKQTQARGEQIEQAL
                     VAIADENGDAIEEYRGRGMVWGIEFRDKDRAGAIAKRAFELGLLIETSGPQSEVVKLLP
                     ALTITADELDEGLRTLARAVRETA"
     aSDomain        3717..4730
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS16830_Aminotran_3.1"
                     /evalue="7.00E-76"
                     /label="IE274_RS16830_Aminotran_3.1"
                     /locus_tag="IE274_RS16830"
                     /protein_end="366"
                     /protein_start="28"
                     /score="247.3"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNLILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDHYLDDSMPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRSLADLCRRQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGMPMALTLFKPELDVWEPGEHNGTFRGNNPAFV
                     TAAATLETYWADGSAMEKQTQARGEQIEQALVAIADENGDAIEEYRGRGMVWGIEFR"
     misc_feature    complement(4954..4969)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
     gene            5001..5402
                     /locus_tag="IE274_RS16835"
                     /old_locus_tag="GCM10010349_34410"
     CDS             5001..5402
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643612.1"
                     /locus_tag="IE274_RS16835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34410"
                     /product="ectoine synthase"
                     /protein_id="WP_190116602.1"
                     /sec_met_domain="ectoine_synt (E-value: 4e-53, bitscore:
                     173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAASGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCTEGVAELTNDETGETFTITPGTMYLLDGHEKHTMRIKEDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPEA"
     gene            5467..6366
                     /gene="thpD"
                     /locus_tag="IE274_RS16840"
                     /old_locus_tag="GCM10010349_34420"
     CDS             5467..6366
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671935.1"
                     /locus_tag="IE274_RS16840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34420"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_190116603.1"
                     /sec_met_domain="PhyH (E-value: 1.1e-39, bitscore: 132.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPGRTADLYPTRGTDEVLIERKDPVVWSAPGTPGPFTGEDLA
                     GFERDGFRAIEQLLTPEEVVEYHAELERLVHDPAMRASERSIVEPRSQEIRSIFEVHKI
                     SEIFGRLAADPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLA
                     NMRTVSVSIALTKNHDTNGGLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDEA
                     LTKLAGEYGIRLFTGEAGSATWFDCNAMHGSGDNITPYPRSNVFLVFNSVENAAVEPFA
                     APVRRPEYIGARDFTPVG"
     gene            complement(6466..7536)
                     /locus_tag="IE274_RS16845"
                     /old_locus_tag="GCM10010349_34430"
     CDS             complement(6466..7536)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643609.1"
                     /locus_tag="IE274_RS16845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34430"
                     /product="alkene reductase"
                     /protein_id="WP_190116604.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLHGVSLTNRIALAPMTRSRAGAGGVPTDLVVEYYAQ
                     RASAGLVITEGVQPSAVGQGYPDTPGLHSAEQIAGWRKVTDAVHAAGGLIFAQLMHAGR
                     IGHPSLLPDGLVNVGPSPIAAEGQVYTHEGPKDYITPRELTGDEVRATIGEFVAAARNA
                     IEAGFDGVELHGANGYLIHQFLAPGSNQRTDEWGGSPAKRIRFAVEVARAVAAEIGADR
                     TGIRLSPGNPFNGIHEPDPGEAYLALVAELNALGLAYLHIMEVGEIRELTQALRKEFSG
                     TFILNPATEGPTGHDDLALIEEGTADLVSFGSLFLANPDLPARLKTRGPYNTPDQSTFY
                     GGDAKGYTDYPALRSA"
     gene            7702..8241
                     /locus_tag="IE274_RS16850"
                     /old_locus_tag="GCM10010349_34440"
     CDS             7702..8241
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643608.1"
                     /locus_tag="IE274_RS16850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34440"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_190116605.1"
                     /transl_table=11
                     /translation="MKADTGTAGAAQADPACIERLPLAAQDGPASMVISRVARLHRIAA
                     GKLLKGLGLYPGQEFVMMHLWDTGPVRQSDLIKVVDLDPSTVTKMLQRLEQAGHVRRRP
                     DPDDRRAVLVEATDSSCGLLTEVRRAWGELEDHTLAGLDPDERTELARLLTRVETNLCV
                     ETADCPEEARREALRG"
     gene            complement(8310..9449)
                     /locus_tag="IE274_RS16855"
                     /old_locus_tag="GCM10010349_34450"
     CDS             complement(8310..9449)
                     /NRPS_PKS="Domain: Aminotran_5 (34-372). E-value: 4.2e-20.
                     Score: 63.9. Matches aSDomain:
                     nrpspksdomains_IE274_RS16855_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143405.1"
                     /locus_tag="IE274_RS16855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34450"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_229909601.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.8e-16, bitscore:
                     53.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METSGQGPVQTVPTSASTSAAFEALVRTEFTARTTYLNSASTGLL
                     PGRAVRAMHEATLSVAEGRPADMFADVEAARAAFARLVGVAPSRVAVGASVAVYSGLVA
                     ASLPAGAEVLTAEGDFSSTVNPFHVRGDLKVRTVPLERIAESVRPGTALVAVSAAQSAD
                     GRIVDLPALRAATRGHGARLYLDASQATGWLDLVEHIADVDFLSSVAFKWLTCPRGVAF
                     FVVPDGPEDPGAPAAFGGVPPLFAGWVAGERPWDSCYGPITELARSARRFDESPSLFAY
                     AGARHSLAVIEELGVAAIRAHDLALADRFRAGLAALGHEPVAAPGSPIVSVPGLGFRQA
                     ELSAAGIEVSDRAGQLRAAFHLYNTEADVDRLLEALGES"
     aSDomain        complement(8334..9347)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE274_RS16855_Aminotran_5.1"
                     /evalue="4.20E-20"
                     /label="IE274_RS16855_Aminotran_5.1"
                     /locus_tag="IE274_RS16855"
                     /protein_end="372"
                     /protein_start="34"
                     /score="63.9"
                     /tool="antismash"
                     /translation="TYLNSASTGLLPGRAVRAMHEATLSVAEGRPADMFADVEAARAAF
                     ARLVGVAPSRVAVGASVAVYSGLVAASLPAGAEVLTAEGDFSSTVNPFHVRGDLKVRTV
                     PLERIAESVRPGTALVAVSAAQSADGRIVDLPALRAATRGHGARLYLDASQATGWLDLV
                     EHIADVDFLSSVAFKWLTCPRGVAFFVVPDGPEDPGAPAAFGGVPPLFAGWVAGERPWD
                     SCYGPITELARSARRFDESPSLFAYAGARHSLAVIEELGVAAIRAHDLALADRFRAGLA
                     ALGHEPVAAPGSPIVSVPGLGFRQAELSAAGIEVSDRAGQLRAAFHLYNTEADVDRL"
     gene            complement(9562..10293)
                     /locus_tag="IE274_RS16860"
                     /old_locus_tag="GCM10010349_34460"
     CDS             complement(9562..10293)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545807.1"
                     /locus_tag="IE274_RS16860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010349_34460"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_190116607.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALKAFPHQDAVEVIHRSFELDP
                     NRPKGDTAAVIPMLAQKYGMTEDQAKDAERRLGDNAAAEGLAYRTEGRDHGNTFDMHRL
                     LHFAKEQGGQAAQDRLIGAFYTANFAEERSVFDDDERLIALAADAGLDAAAARGVLADP
                     DAYADAVRADEREAAELGANGVPFFVLDRKFGVSGAQPAEVFTQALEQAWGARSPLTVL
                     AEEGADGDACGPDGCAVPPRD"
ORIGIN
        1 gccccgggcg cgcgccgttg gaggtcctgc cggaggggct agagaatgtg ccggtgagta
       61 tcagcccgtc cggcgcttga ggacgaggcg cgaagcgccg atcagcgggg gtccgggggc
      121 ggcagccccc gggttcggga aggggcggga ttggggagcc cccggcaagg cccccggcct
      181 cagcccacca ccctcccgtt caccaccacc gcggaaggcg ccgccagcac ccgcatatcg
      241 gcccgcggat cctccccgta cacgacgagg tcagccggag ccccctcgac gagcccgggc
      301 cgccccagcc agtcccgcgc acgccacacg gcggcggaaa gcgcgtcgac gggcgggatg
      361 cccgccttgg tgagctcggc gacctccccg gcgatgagcc cgtgggcgag ggacccgccg
      421 gcgtcggtac cggcgtagac ggggacgccg gcgtcatagg cggcgcggac ggtgtcgtag
      481 cgccgggcgt gcagccgccg catatgggcg gaccagcgcg ggaacttggc ttcgcccccg
      541 tcggcgagct gcgggaaggt ggcgatgttg acgagggtgg ggacgatggc gacgccgcgg
      601 gcggcgaaga gggggatggt gtcctcggtg agcccggtgg cgtgctcgac gcagtcgatc
      661 ccggcttcga cgaggtcggg cagggagtcc tcggcgaagc agtgggcggt gacgcgggcg
      721 ccgagccggt gggcttcggc gatggcggcc tcgacggccc cgcggggcca gcaggcggtg
      781 aggtccccgg cgtcgcggtc gatccagtcg ccgacgagct tgacccagcc gtcgccgcgc
      841 cgcgcctcgc gggcgacgta ggcgacgagg tcgtcgggct cgatctcgtg ggcgtagttg
      901 cggatgtagc ggcgggtacg ggcgatgtgc cggccggcgc ggatgatttt gggcaggtcc
      961 tcgcggtcgt cgatccagcg ggtgtcggag ggcgaacccg cgtcgcgcag gaggagggtg
     1021 cccgcgtcgc ggtcggtgcg ggcctgcttc tcggcgacgt ccggcgcgac gggcccgtgg
     1081 gcgtcgaggc cgacgtggca gtgggcgtcg acgaggccgg gcagcgccca gccgctgacg
     1141 gtctccacgt ccccggcggc gggccgccgg taggtcaccc gccccccgac cacccacagc
     1201 tcgtcgcgga cgtcctcggg ccctacgagc acccgcccct tcacgtgcag caccgcgcgt
     1261 tcgctcatgg ccgccagctt agacagcagc gtgggaggcg acagggctga ctcgggcggt
     1321 gggtacgctc gacggcgagg ggcgcgggcc cgggcccgcg gccgcctcac cgaccggccg
     1381 tagagacccc gttaccgaag cgagcagcag cgtgactcac cccttccttg acctggcgcc
     1441 gctgggcgcc gcgcacttcg ccgccatcga ggaccgggtg gcccggctcc tggacaccga
     1501 gcaggacgtc gtgatcatgc agggcgaggc gctgctgccc ctggagggct gtatccgggg
     1561 cgccgccagg cccggcacga aggccctgaa cgtgatcacc ggcccgtacg ggcagacgtt
     1621 cgggaactgg ctgcgggact gcggcgcgac ggtcgtggac ctggcggtgc cgttccacac
     1681 ggcggtgacg gccgcgcagg tcgaggaggc gttcgccgcg cacccggaca tcgacttcgt
     1741 gtccctggtg cacgcggagg cggcgaccgg caacaccaac cccgtggcgg ggatcggcga
     1801 ggtcgtgcgg gcgcacggcg cgctgttcat gctggacgcg gtggcgtcgg tggccgccga
     1861 gccgctgctg ccggacgcgt ggggcgtgga cctgtgcgtg atcggcgcgc agaaggcgat
     1921 gggcggtccg gcgggggttt cggcggtgtc ggtgagcgag cgcgcctggc agcgcatggc
     1981 cgcgaacccg caggcgccgc gccgctcgta cctctccctc ctggactgga aggagcgctg
     2041 gatcgacggc ggccgcaagg cgctcctgca cgccccggcg cagttggaga tgctggccct
     2101 ggaggcgtgt gtggagcgca tcgaggcgga gggcctgacc gccctgatgg ggcggcacgc
     2161 gacggccgcg acggcgacgc gcgcgggcgc cctggcgctc ggcgggggtc tggagccgta
     2221 tgtgtacgag gccggggacg ccgcgccggt cgcgacgacg ctgcgggccc cgcggggcgt
     2281 ggacgccgcc gacctggtcg cgcgggcgct ggccgccgat ccggtgcttc cgctggtcgc
     2341 gggcggtggc gcgctggcca aggagatgat ccgcgtcaac cactacggtc cggacgcgac
     2401 gcgcggcgtc gtgcacgcgt cactggcggc gctgggtgcg gcgatgggcg aggtcgggct
     2461 cggcgtggac ctggaggccg cccgccgcgc cgtcaccgac acctgggcgt aggcctggtt
     2521 gagcgccgat cgcgctccgc gctcgtcctc aagcgccgga cgggctcttc gagcccgccg
     2581 caccatgaac gggacagccc gtccggcgtc tgccgaaatc atgattcacg cactgcgata
     2641 aagcgctcca gggcaatttc cccgtgcgct ttcatgaatt ctttatgagt gcagcttccc
     2701 gtattcttct gcccgctttc cgaggcccta aacacaaagg tttcgccagc accaaccacc
     2761 gtaattcggg cacgtctcat cgagattaca cggctgtgac gtactccaca gcaagcccgt
     2821 tttgtcggaa gtaaatccgt cgataccgtc acaaaacccc agcgattcat gcgggcgccc
     2881 gcgcctgcgt gataacacac gagagcgccg cgcgtaaccc cttcccccga tgcaatccca
     2941 gaaattgctc ggtaaattca atccgcatga ccgctgcaca agccgatcta ccgaccgacc
     3001 tgcaagcgaa attccttgaa atgcccgagg gcctgcggct cgacaccccg gaggtcgccg
     3061 acggcgccgc catctggcgg atcgcccgcg actcgaaggt cctcgacctg aactcctcgt
     3121 acagctacct gctgtggtgt cgcgacttcg cggcgacgtc cgtcgtggcc agggacaccg
     3181 gcggcgagac cgtcggcttc atcaccggct acatccgccc cgacaagccg cgcaccctgg
     3241 tggtctggca ggtggccgtc gaccacgcgc agcgtggccg gggcctcgcc ggtgcgctgc
     3301 tcgacggcct ggccgcgaag gtcgcggacc ggcacgggtt cgacgccctg gagacgacca
     3361 tcacgccggg caacaccgca tccgagcgcc tgttcacctc cttcgcggag cggcacgggg
     3421 cgcgcgtcga gcggaccgtg ctgttcgacg cggccctgtt ccccggcgac gacggcgggc
     3481 acgagcccga ggtcctgtac gacatcgcgc cactcttccg ctgaccggcc ggcagccgcg
     3541 cccgtgggcc tgacgcaccg tcacatgccc ctggcgccgt cccagtccac tcagaacccc
     3601 gcacgaccaa tccatctccc aggagatccg ctgtgaccat cacccagccc gacctgagcg
     3661 tcttcgagac cctggagtcg gaggtgcgca gctactgccg cggttggccc accgtcttcg
     3721 accgcgcgca gggcagccgc atgttcgacg aggacggcca tgagtacctc gacttcttcg
     3781 ccggggccgg ctcgctcaac tacggccaca acaacccggt cctcaaacga gccctcatcg
     3841 actacctgga gcgcgacggc gtcacgcacg gcctcgacat gtcgacgacg gccaagcgcg
     3901 cgttcctgga gtcgttccag aacctgatcc tgcgcccgcg cgacctgccg tacaaggtca
     3961 tgttcccggg cccgacgggc accaacgcgg tggagtccgc gctgaagctg gcccgcaagg
     4021 tcaagggccg cgagtcgatc gtgtccttca ccaacgcctt ccacggcatg tcgctcggct
     4081 cgctcgcggt gaccggcaac gccttcaagc gggcgggcgc gggcatcccg ctggtgcacg
     4141 gcacgccgat gcccttcgac cactacctcg acgactcgat gccggacttc ctgtggttcg
     4201 agcggctcct ggaggaccag ggctcgggcc tgaacaagcc cgccgcggtg atcgtggaga
     4261 ccgtgcaggg cgagggcggc atcaacgtcg cgcgcgccga gtggctgcgc tcgctcgccg
     4321 acctgtgccg ccgccaggac atgctcctga tcgtcgacga catccagatg ggctgcggcc
     4381 gcaccggcgc gttcttctcc ttcgaggagg cgggcatcgt gccggacatc gtcacggtgt
     4441 ccaagtccat cagcggctac ggcatgccga tggcgctcac gctcttcaag ccggagctcg
     4501 acgtgtggga gccgggcgag cacaacggca ccttccgcgg caacaacccg gcgttcgtga
     4561 cggccgccgc caccctggag acctactggg ccgacggctc ggcgatggag aagcagaccc
     4621 aggcccgcgg cgagcagatc gagcaggcgc tggtcgccat cgccgacgag aacggcgacg
     4681 ccatcgagga gtaccgcggc cgcggcatgg tgtggggcat cgagttccgt gacaaggacc
     4741 gcgcgggcgc catcgccaag cgcgccttcg aactcggcct gctcatcgag acgtccggcc
     4801 cgcagagcga ggtcgtcaag ctgctcccgg ccctgaccat cacggccgac gagctggacg
     4861 agggcctgcg cacgctcgcc cgcgcggtcc gcgagaccgc ttgagcggac gcccgcgccg
     4921 cgcccccggc cgcgtaccca cgcgtaccgc acccgtgaca gcacgcacag aaacacgcac
     4981 agaagaggaa ctgactgacc gtgatcgtcc gctcgttcaa ggacattgaa ggcaccgacc
     5041 ggcacgtcaa ggccgcgtcg ggcacctggg agagcaagcg catcgtgctc gccaaggagc
     5101 gcgtcggctt ctcgttgcac gagaccgtgc tctacgccgg tacggagacg agcatgtggt
     5161 acgccaacca catcgaggcc gtcgtctgca ccgagggcgt ggccgagctg accaacgacg
     5221 agaccggcga gaccttcacc atcacgccgg gcacgatgta cctgctcgac gggcacgaga
     5281 agcacacgat gcgcatcaag gaggacttcc gctgcctgtg cgtgttcaat ccgccggtga
     5341 cgggccgcga ggaccacgac gagaacggcg tgtacccgct gctcaccgag ccggaggcgt
     5401 gatccgccgc tgacggaaga gacatcccgg acagggccgt acgtgagagg agaggcaggc
     5461 aacaccatga ccaccgcacc cggacgaacc gccgacctgt acccgacccg aggcaccgac
     5521 gaggtcctca tcgagcgcaa ggaccccgtg gtgtggtccg cgccgggtac gccggggccc
     5581 ttcaccggcg aggacctggc ggggttcgaa cgcgacggct tccgggccat cgagcaactg
     5641 ctcacgcccg aggaagtcgt ggagtaccac gccgagttgg agcggctggt ccacgacccg
     5701 gcgatgcgcg cgagcgagcg ctccatcgtc gagccgcgct cccaggagat ccggtcgatc
     5761 ttcgaggtgc acaagatcag tgagatcttc ggccgcctcg ccgccgaccc gcgggtggtg
     5821 ggccgcgccc gccagatcct cggctccgac gtgtacgtcc accagtcccg gatcaacgtc
     5881 aagccgggct tcggggcgtc gggcttctac tggcactcgg acttcgagac ctggcacgcc
     5941 gaggacggcc tcgcgaacat gcggaccgtg tcggtgtcga tcgcgctcac gaagaaccac
     6001 gacaccaacg gcgggctcat gatcatgccc ggctcgcacc ggacgttcct cggctgcgcg
     6061 ggcgcgacgc cgaaggacaa ctacaagaag tcgctgcaga tgcaggacgc gggcacgccg
     6121 tccgacgagg cgctgaccaa gctcgccggg gagtacggca tccgcctgtt caccggcgag
     6181 gcgggctcgg cgacctggtt cgactgcaac gccatgcacg gctccggcga caacatcacg
     6241 ccctacccgc gctcgaacgt cttcctcgtc ttcaacagcg tggagaacgc ggcggtcgag
     6301 ccgttcgcgg cgccggtgcg gcggcccgag tacatcgggg ccagggactt cacaccggtg
     6361 gggtgacgtc ctgacgcgct gagccgcacg gccgacggcg gtggcccggc accttctccg
     6421 gtgccgggcc accgccgtcg ccgtttccgc tcgcgggata ccgggtcacg cagaccgcag
     6481 cgccgggtag tccgtgtagc ccttggcgtc gccgccgtag aacgtggact ggtccggcgt
     6541 gttgtacgga ccgcgggtct tcagacgggc cgggaggtcc gggttggcga ggaacaggga
     6601 gccgaaggag accaggtcgg cggtgccttc ctcgatcagg gccaggtcgt cgtgaccggt
     6661 gggaccctcg gtcgcggggt tgaggatgaa cgtgccgctg aactccttgc gcagcgcctg
     6721 cgtcagctcg cggatctcac cgacctccat gatgtgcaga taggcgaggc cgagggcgtt
     6781 cagctccgcc accagggcga ggtaggcctc ccccgggtcg ggctcgtgga tgccgttgaa
     6841 ggggttgccg ggcgagaggc ggatacccgt gcggtcggcg ccgatctcgg cggcgacggc
     6901 cctggcgacc tcgacggcga agcggatgcg cttcgccggg gagccgcccc actcgtcggt
     6961 gcgctggttg gagcccggcg cgaggaactg gtggatcaga tagccgttgg cgccgtgcag
     7021 ctcgacgccg tcgaagcccg cctcgatggc gttgcgggcc gccgccacga actcgccgat
     7081 ggtggcgcgc acctcgtcgc cggtcagctc gcggggcgtg atgtagtcct tcggtccctc
     7141 gtgggtgtag acctgcccct cggcggcgat cggggacggg ccgacgttca ccagtccatc
     7201 gggcagcagc gaggggtggc cgatgcggcc cgcgtgcatg agctgggcga agatcaggcc
     7261 gcccgcggcg tgcacggcgt cggtgacctt gcgccagccc gcgatctgct cggcgctgtg
     7321 caggcccggg gtgtccgggt agccctggcc cacggcggag ggctgcacgc cctccgtgat
     7381 gaccaggccg gcgctggcgc gctgggcgta gtactcgacg acgaggtcgg tggggacgcc
     7441 gcccgcgccc gcccggctgc gggtcatcgg cgccagggcg atgcggttgg tgagggacac
     7501 gccgtgcagg tcgatggggt cgaatgcggt ggtcatcaca gctccagggc gtacgaaggt
     7561 tggtcggcca agtaattcaa ccgcgagcca ctgtagcgca ttccttggcc gaccaagtta
     7621 atctctggag ggaaccagga cgacactggt ggaggatggc gacggcggac agcgccggac
     7681 cggcgaaggg caggagctgc gatgaaggcc gacacaggga cggcgggggc cgcgcaggcc
     7741 gaccccgcgt gtatcgagcg gctgccgctg gccgcccagg acgggccggc cagcatggtg
     7801 atcagccggg tggcccggct gcaccggatc gcggcgggca agctcctgaa ggggctcggc
     7861 ctctaccccg gccaggaatt cgtgatgatg cacctgtggg acacgggccc ggtgcgccag
     7921 tccgacctga tcaaggtcgt cgacctcgac ccgtcgacgg tcaccaagat gctgcagcgc
     7981 ctggaacagg ccgggcacgt acggcggcgg cccgacccgg acgaccgccg ggccgtgctc
     8041 gtcgaggcga ccgattcgag ctgcgggctg ctcaccgaag tgcggcgcgc ctggggcgag
     8101 ttggaggacc acaccctcgc cggtctggac ccggacgagc ggacggagct ggcccggctg
     8161 ctcacccggg tcgagaccaa cctctgcgtg gagacggcgg actgcccgga ggaggcgcgg
     8221 cgggaggcgc tgcgcgggta gggcagcggg tccggggcag ggcggcctca gcccgccccg
     8281 gtcccggccc tccggcccgc cccggctcct cagctctcgc cgagagcctc caggagccgg
     8341 tccacgtcgg cctccgtgtt gtacaggtgg aaggccgccc gcagttggcc cgcgcggtcc
     8401 gacacctcga tgcccgccgc gctcaactcc gcctggcgga aacccagtcc gggcaccgac
     8461 acgatgggcg acccgggcgc ggccaccggc tcgtggccga gcgccgcgag accggcgcgg
     8521 aagcggtccg cgagcgccag gtcatgggcg cggatcgcgg ccacccccag ctcctcgatc
     8581 acggccaggg agtgccgggc gcccgcgtac gcgaacaggc tcggactctc gtcgaaccgc
     8641 cgtgcggagc gggctagttc ggtgatcggg ccgtagcagc tgtcccaggg acgctcgccc
     8701 gcgacccagc ccgcgaagag cgggggcaca ccgccgaagg ccgcgggcgc gccggggtcc
     8761 tccgggccgt cggggaccac gaagaacgcg acgccccgcg gacaggtgag ccacttgaag
     8821 gccaccgacg acaggaagtc cacgtcggcg atgtgctcga cgaggtccag ccagcccgtc
     8881 gcctgcgagg cgtccaggta gaggcgggcg ccgtgcccgc gggtcgcggc gcgcagcgcg
     8941 ggcaggtcga cgatccggcc gtccgccgac tgggccgcgg acaccgcgac cagggcggtg
     9001 cccgggcgca cggactccgc gatccgctcc agcggcacgg tacggacctt caggtcgccg
     9061 cgtacgtgga aggggttcac cgtcgagctg aagtcgccct cggcggtgag gacttcggcg
     9121 cccgccggca gcgaggcggc gaccagaccg ctgtacacgg cgacggacgc gcccaccgcg
     9181 acccggctcg gcgccactcc gaccaggcgg gcgaaggcgg cgcgggccgc ctccacgtcc
     9241 gcgaacatgt cggcggggcg gccctccgcc accgacaggg tcgcctcgtg catggcgcgg
     9301 acggcgcggc ccggcagcag gccggtgctc gcgctgttca ggtaggtggt cctggcggtg
     9361 aactcggtac gtacgagcgc ttcgaacgcg gcggaggtcg aggcggaggt cgggacggtc
     9421 tggacggggc cctggccgga agtctccatg aggtcactgt gccgcctcgg ccgggcccgg
     9481 tccattgcgg atgcctgacc cgtaccgctt agcgatgctt acccgtgggc cctgaccagg
     9541 gcttccgccg tccggcgcag atcagtcgcg gggcgggacc gcgcagccgt cggggccgca
     9601 ggcgtcgccg tcggcgcctt cctccgcgag caccgtcagc ggtgagcgcg cgccccacgc
     9661 ctgctccagg gcctgcgtga agacctcggc gggctgggcg ccggagacgc cgaacttccg
     9721 gtcgagcacg aagaacggca cgccgttcgc gcccagctcg gcggcctcgc gctcgtccgc
     9781 gcggaccgcg tcggcgtacg cgtcggggtc ggcgaggacc ccgcgggccg ccgccgcgtc
     9841 gaggcccgcg tccgccgcga gggcgatcag gcgctcgtcg tcgtcgaaga ccgagcgctc
     9901 ctcggcgaag ttggcggtgt agaacgcgcc gatgaggcgg tcctgggcgg cctggccacc
     9961 ctgctccttg gcgaagtgca gcaggcggtg catgtcgaag gtgttgccgt ggtcgcggcc
    10021 ctcggtgcgg tacgcgagac cctcggccgc cgcgttgtcg ccgaggcggc gctcggcgtc
    10081 cttcgcctgg tcctccgtca tgccgtactt ctgcgccagc atcgggatca cggccgccgt
    10141 gtcgcccttc gggcggttcg ggtccagctc gaaggagcgg tggatcacct ctacggcgtc
    10201 ctggtgcggg aaggccttca gggccttctc gaagcgggcc ttgcccacgt agcaccaggg
    10261 gcaggcgatg tcgctccaga tctcgacgcg catgtggttc ttcctcgggt cgtacgaaac
    10321 ggagacgtcc tccgcgtctg tgcaacccgg ggccggagtg ttccattccc acccggcggc
    10381 ggtcaccccg ggcgggggcc ga
//
