LOCUS       NZ_BMVQ01000053         2907 bp    DNA     linear   CON 11-FEB-2024
DEFINITION  Streptomyces anthocyanicus strain JCM 4739 sequence53, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVQ01000053
VERSION     NZ_BMVQ01000053.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces anthocyanicus
  ORGANISM  Streptomyces anthocyanicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceoruber group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:14:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,934
            CDSs (total)                      :: 7,858
            Genes (coding)                    :: 7,681
            CDSs (with protein)               :: 7,681
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 73 of 177
            Pseudo Genes (incomplete)         :: 130 of 177
            Pseudo Genes (internal stop)      :: 16 of 177
            Pseudo Genes (multiple problems)  :: 37 of 177
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:48:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2907
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVQ01000053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..2905)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (202-271). E-value: 1.4e-29. Score:
                     94.1. Matches aSDomain: nrpspksdomains_IE283_RS39260_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (284-585). E-value:
                     6.7e-119. Score: 388.3. Matches aSDomain:
                     nrpspksdomains_IE283_RS39260_Condensation_LCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028843.1"
                     /locus_tag="IE283_RS39260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_193487686.1"
                     /sec_met_domain="PP-binding (E-value: 8.3e-20, bitscore:
                     57.4, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.1e-74, bitscore:
                     237.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.7e-47, bitscore:
                     147.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVPIGRPFANTQVYVLDSALRPVAPGTTGELYLAGEQLARGYLG
                     RPALTAERFTANPHSSTPGARMYRTGDLAHWNHDGHLTYDGRADHQIKLRGHRIEPGEI
                     ETTLTAQTGITQATVQLREDQPGDQRLVAYLVVNDSTGYDEKTVRDALTSALPDYMVPS
                     ALVTLDALPLTPNGKLDRTALPAPAYSASTAGRAPRTPREEVLCTLFAEVLGVDLVTID
                     DNFFDLGGHSLLATRLVSRTRTALGVELSIRQLFETPTVAGLAEALDASGTVRTALTAR
                     PRPERIPLSYAQQRLWFLHQLEGPSATYNTVLTLRLGGALDVDALRAAIGDVVARHESL
                     RTVFTEDERGAYQIVLPVEAASTPFTVVDVAEEEIGDQLDEAVGHCFDLAQELPARTSL
                     FRVSEREHVLLLLIHHIASDAWSRAPLAQDLTAAYAARVRSEAPMWAPLTVQYADYALW
                     QQEILGDDTDADSLAGRQLAYWKQQLAGLPEQLDLPTDRPRPAVAGYSGDRVPFTVPTE
                     LHTRLTELARATNTSAFMVIQAAVAVLLTRLGAGEDIPIGTPVAGRTDDAADDLIGLFI
                     NTLVLRTDTSGDPTFRRLLDRVRDTDLAAYAHQDLPFERLVEALNPARTLSHHPLFQVL
                     LTFNNTDHEGALKDISELPGLTVALREVQRTSSKFDLSFGFAESFDTSRRPQGIEAALD
                     FSTELLDRRSAQAIADRFLRVLEAVTTAPDRPIGAVELMDAAERERVLVEWNGAPTELP
                     GTPLHELISEQARLTPDAVAVVCDGTTLTYAELDRRANQLARHLLGEGLGAEDFVAIAL
                     AKSLDAVISMLAVLKTGAAYLPIDPDYPAERITYMLDDAQPALTLTAPIPPASYDSRPT
                     SEITDVERRSPWSARHAAYMIYTSGSTGRPKGVVIEHHALATYLHRARNTYTAMTGVTV
                     LHSPLAFDLTITALWTPLTAGGTVHLTSLEEAEVQPSL"
     source          1..2907
                     /culture_collection="JCM:4739"
                     /db_xref="taxon:68174"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces anthocyanicus"
                     /strain="JCM 4739"
                     /submitter_seqid="sequence53"
                     /type_material="type strain of Streptomyces coelescens"
     gene            complement(<1..>2907)
                     /locus_tag="IE283_RS39260"
     region          1..2907
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2907
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2907
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2905](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2905)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(181..240)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0011"
                     /evalue="6.70E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="IE283_RS39260"
                     /protein_end="908"
                     /protein_start="888"
                     /score="33.9"
                     /tool="antismash"
                     /translation="AYMIYTSGSTGRPKGVVIEH"
     CDS_motif       complement(397..438)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0010"
                     /evalue="2.40E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="IE283_RS39260"
                     /protein_end="836"
                     /protein_start="822"
                     /score="17.5"
                     /tool="antismash"
                     /translation="LAVLKTGAAYLPID"
     CDS_motif       complement(991..1110)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0009"
                     /evalue="2.40E-24"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE283_RS39260"
                     /protein_end="638"
                     /protein_start="598"
                     /score="77.9"
                     /tool="antismash"
                     /translation="RDTDLAAYAHQDLPFERLVEALNPARTLSHHPLFQVLLTF"
     aSDomain        complement(1150..2052)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE283_RS39260_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="6.70E-119"
                     /label="IE283_RS39260_Condensation_LCL.1"
                     /locus_tag="IE283_RS39260"
                     /protein_end="585"
                     /protein_start="284"
                     /score="388.3"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIASDA"
                     /tool="antismash"
                     /translation="ERIPLSYAQQRLWFLHQLEGPSATYNTVLTLRLGGALDVDALRAA
                     IGDVVARHESLRTVFTEDERGAYQIVLPVEAASTPFTVVDVAEEEIGDQLDEAVGHCFD
                     LAQELPARTSLFRVSEREHVLLLLIHHIASDAWSRAPLAQDLTAAYAARVRSEAPMWAP
                     LTVQYADYALWQQEILGDDTDADSLAGRQLAYWKQQLAGLPEQLDLPTDRPRPAVAGYS
                     GDRVPFTVPTELHTRLTELARATNTSAFMVIQAAVAVLLTRLGAGEDIPIGTPVAGRTD
                     DAADDLIGLFINTLVLRTDT"
     CDS_motif       complement(1159..1248)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0008"
                     /evalue="1.40E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE283_RS39260"
                     /protein_end="582"
                     /protein_start="552"
                     /score="46.4"
                     /tool="antismash"
                     /translation="DIPIGTPVAGRTDDAADDLIGLFINTLVLR"
     CDS_motif       complement(1516..1554)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0007"
                     /evalue="1.20E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE283_RS39260"
                     /protein_end="463"
                     /protein_start="450"
                     /score="17.8"
                     /tool="antismash"
                     /translation="QYADYALWQQEIL"
     CDS_motif       complement(1603..1668)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0006"
                     /evalue="5.70E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE283_RS39260"
                     /protein_end="434"
                     /protein_start="412"
                     /score="28.5"
                     /tool="antismash"
                     /translation="IHHIASDAWSRAPLAQDLTAAY"
     CDS_motif       complement(1873..1986)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0005"
                     /evalue="3.50E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE283_RS39260"
                     /protein_end="344"
                     /protein_start="306"
                     /score="54.8"
                     /tool="antismash"
                     /translation="ATYNTVLTLRLGGALDVDALRAAIGDVVARHESLRTVF"
     CDS_motif       complement(2005..2037)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0004"
                     /evalue="1.40E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="IE283_RS39260"
                     /protein_end="300"
                     /protein_start="289"
                     /score="11.1"
                     /tool="antismash"
                     /translation="SYAQQRLWFLH"
     aSDomain        complement(2092..2298)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE283_RS39260_PCP.1"
                     /evalue="1.40E-29"
                     /label="IE283_RS39260_PCP.1"
                     /locus_tag="IE283_RS39260"
                     /protein_end="271"
                     /protein_start="202"
                     /score="94.1"
                     /tool="antismash"
                     /translation="EEVLCTLFAEVLGVDLVTIDDNFFDLGGHSLLATRLVSRTRTALG
                     VELSIRQLFETPTVAGLAEALDAS"
     CDS_motif       complement(2260..2292)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0003"
                     /evalue="5.80E-03"
                     /label="Cy4"
                     /locus_tag="IE283_RS39260"
                     /protein_end="215"
                     /protein_start="204"
                     /score="9.8"
                     /tool="antismash"
                     /translation="VLCTLFAEVLG"
     CDS_motif       complement(2590..2655)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0002"
                     /evalue="3.60E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="IE283_RS39260"
                     /protein_end="105"
                     /protein_start="83"
                     /score="35.1"
                     /tool="antismash"
                     /translation="YDGRADHQIKLRGHRIEPGEIE"
     CDS_motif       complement(2743..2829)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE283_RS39260_0001"
                     /evalue="8.20E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="IE283_RS39260"
                     /protein_end="54"
                     /protein_start="25"
                     /score="50.1"
                     /tool="antismash"
                     /translation="PGTTGELYLAGEQLARGYLGRPALTAERF"
ORIGIN
        1 atcaggctgg gctggacctc agcctcctcc aggctggtca ggtggaccgt gccgccggcg
       61 gtgagaggcg tccacagggc ggtgatggtg aggtcgaagg ccaggggtga gtgcaggacg
      121 gtcacgccgg tcatggcggt gtaggtgttg cgggcgcggt ggaggtaggt ggccagggcg
      181 tggtgctcga tgacgacgcc cttggggcgg ccggtcgagc cggaggtgta gatcatgtac
      241 gcggcatggc gtgccgacca ggggctacgg cgctccacat cggtgatctc gctggtgggc
      301 cggctgtcgt acgaagcggg cggtatgggc gcggtgaggg tcagggccgg ctgggcgtcg
      361 tcgagcatgt aggtgatgcg ttcggcgggg tagtccgggt cgatgggcag gtaggccgcg
      421 ccggtcttga gcacggccag catgctgatc accgcgtcca gcgacttggc cagggcgatc
      481 gcgacgaagt cctccgcacc gaggccttct cccagcaggt gccgggcgag ctggttggcg
      541 cgccggtcca gctcggcgta ggtcagggtg gtgccgtcgc acaccaccgc cacggcgtcc
      601 ggcgtcaacc gggcctgctc gctgatcagt tcgtgcagcg gtgtcccggg aagctcggtc
      661 ggggccccgt tccactccac caggacccgc tcccgctcgg ccgcgtccat cagctcgacg
      721 gcgccgatcg gccggtccgg ggctgtggtg acggcttcca gcacccgcag gaaccggtcc
      781 gcgatcgcct gggcggaccg gcggtcgagg agttccgtac tgaagtccag cgccgcttcg
      841 atgccttgtg gccgacgaga cgtgtcgaag gactccgcga acccgaagga gaggtcgaac
      901 ttcgacgagg tccgctggac ctcccgaagt gcgacggtga gccccggaag ctcggagatg
      961 tccttcagcg cgccttcgtg gtccgtgttg ttgaacgtca gcaggacctg gaagagcggg
     1021 tgatgggaca gcgtgcgtgc cgggttcagc gcctcgacca gccgctcgaa ggggaggtcc
     1081 tggtgcgcgt aggcggccag gtcggtgtcc cgcacgcggt cgaggagccg gcggaaggtc
     1141 gggtcaccgg acgtgtcggt gcggaggacc agcgtgttga tgaagagccc gatcaggtcg
     1201 tcggcggcgt cgtccgtgcg tccggcgacg ggggtgccga tcggaatgtc ctcaccggcg
     1261 cccagacggg tgagcaggac ggcgaccgca gcctggatca ccatgaaggc actggtgttg
     1321 gtggcgcgcg ccagctccgt cagccgcgtg tgcagctccg tcggcacggt gaacggaaca
     1381 cggtcccccg agtaccccgc gaccgccggt cggggccggt cggtggggag gtcgagctgt
     1441 tcgggcaggc cggcgagctg ctgtttccag taggcgagct ggcggcccgc gaggctgtcg
     1501 gcatcggtgt cgtcgccgag gatctcctgc tgccagagcg cgtagtcggc gtactggacg
     1561 gtgagcgggg cccacatggg cgcctcgctg cggacgcggg ccgcgtaggc ggcggtgagg
     1621 tcctgggcca gtggggcgcg ggaccaggcg tcactggcga tgtggtggat gaggagcagc
     1681 agtacgtgct cccgctccga cacccggaac agcgaggtgc gcgcgggaag ttcctgggcg
     1741 aggtcgaagc agtggccgac ggcttcgtcg agctggtcgc cgatctcctc ctcggccacg
     1801 tcgacgacgg tgaagggcgt cgaggccgcc tccacgggca gcacgatctg gtacgcgcct
     1861 cgctcgtcct cggtgaagac cgtccgcagg ctctcgtgcc gggcgacgac gtcaccgatc
     1921 gccgcgcgca gggcgtccac gtcgagcgcg ccgccgagcc gcagcgtgag cacggtgttg
     1981 taggtcgccg atggtccttc gagctggtgg aggaaccaga ggcgttgctg ggcgtaggag
     2041 agcgggatgc gctcggggcg cggcctcgcg gtgagcgcgg tgcggacggt gcccgacgcg
     2101 tcgagggcct cggcgagacc cgccacggtc ggcgtctcga agagctgccg gatggacagt
     2161 tcgacgccga gtgcggtgcg ggtgcgggag acgaggcggg tggcgaggag ggagtgtccg
     2221 ccgaggtcga agaagttgtc gtcgatggtg acgaggtcga cgccgaggac ttcggcgaag
     2281 agtgtgcaga ggacttcttc gcggggggtg cgaggtgctc gtccggcggt ggatgccgag
     2341 taggcggggg cggggagggc ggtgcggtcg agtttgccgt tgggggtgag ggggagggcg
     2401 tcgagggtga cgagtgcgct ggggaccatg tagtcgggca gtgcgctcgt gagtgcgtcg
     2461 cgcacggttt tctcgtcgta tccggtggag tcgttgacga cgaggtaggc gacgaggcgc
     2521 tggtcgcccg gctggtcctc acggagttgg acggtggctt gggtgatgcc ggtctgtgcg
     2581 gtgagggtgg tttcgatttc gccgggttcg atgcggtggc cgcggagttt gatctggtgg
     2641 tcggcgcgtc cgtcgtaggt gaggtggccg tcgtggttcc agtgggcgag gtcgccggtg
     2701 cggtacatgc gggcgccggg ggtgctgctg tgggggttgg cggtgaagcg ttcggcggtg
     2761 agggcggggc ggccgaggta gccgcgggcg agttgttctc cggcgaggta cagctctccg
     2821 gtggtgccgg gggcgacggg ccgcagggcc gagtcgagga cgtagacctg ggtgttggcg
     2881 aagggccgtc cgatggggac gggaccg
//
