LOCUS       NZ_BMVQ01000008        10399 bp    DNA     linear   CON 11-FEB-2024
DEFINITION  Streptomyces anthocyanicus strain JCM 4739 sequence08, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVQ01000008
VERSION     NZ_BMVQ01000008.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces anthocyanicus
  ORGANISM  Streptomyces anthocyanicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceoruber group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 418380)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:14:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,934
            CDSs (total)                      :: 7,858
            Genes (coding)                    :: 7,681
            CDSs (with protein)               :: 7,681
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 73 of 177
            Pseudo Genes (incomplete)         :: 130 of 177
            Pseudo Genes (internal stop)      :: 16 of 177
            Pseudo Genes (multiple problems)  :: 37 of 177
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:48:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 73880
            Orig. end                         :: 84279
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVQ01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(852..1334)
                     /locus_tag="IE283_RS21705"
                     /old_locus_tag="GCM10010348_43910"
     CDS             complement(852..1334)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028017.1"
                     /locus_tag="IE283_RS21705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_43910"
                     /product="hypothetical protein"
                     /protein_id="WP_189284571.1"
                     /transl_table=11
                     /translation="MFLVLLLIVVVAVVSAVGLTGHGASVLAARRARGTGAGPALRALA
                     AFAGAAAVAMYAWGLLHVTGAVMESDGGAGSAPAPPCRTPGNEERALHVIDQSVSFLPL
                     GFVCETTGGGEYTSDDVPGYVNPATAALALTAVAGAVGAGYASEARARATARGGGG"
     gene            1512..2639
                     /locus_tag="IE283_RS21710"
                     /old_locus_tag="GCM10010348_43920"
     CDS             1512..2639
                     /NRPS_PKS="Domain: Aminotran_5 (51-291). E-value: 1.1e-27.
                     Score: 88.8. Matches aSDomain:
                     nrpspksdomains_IE283_RS21710_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329315.1"
                     /locus_tag="IE283_RS21710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_43920"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_003976957.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.5e-21, bitscore:
                     71.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLSAGRFAAIEDRVARLLGTGQDVVVMQGEALLPLE
                     GAIRAAAGPGTVALNVVTGPYGQTFGNWLRDCGATVHDLAVPFHTAVSAEQVGRALAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKALLHAP
                     AQLEMLALEACVERIEAEGPGTVMARHASAAAATRAGALALGGLEPYVYEAGDAAPVAT
                     TLRTPSDVDASALVARALESDPALPLAAGGGALAGEMIRVNHYGPDATRGAVQGCLAAL
                     GAALVERGVKADPEAARRAAEEAWERATGPGLSEG"
     aSDomain        1665..2384
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE283_RS21710_Aminotran_5.1"
                     /evalue="1.10E-27"
                     /label="IE283_RS21710_Aminotran_5.1"
                     /locus_tag="IE283_RS21710"
                     /protein_end="291"
                     /protein_start="51"
                     /score="88.8"
                     /tool="antismash"
                     /translation="AGPGTVALNVVTGPYGQTFGNWLRDCGATVHDLAVPFHTAVSAEQ
                     VGRALAEHPEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPD
                     AWGVDLCVIGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGG
                     RKALLHAPAQLEMLALEACVERIEAEGPGTVMARHASAAAATRAGALALGGLEPYVYEA
                     GDAAPVATTLRTPSDVDA"
     misc_feature    complement(2674..2687)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.99"
                     /tool="antismash"
     misc_feature    complement(2788..2803)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2798..2813
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2932..2944
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    2958..2970
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            2969..3481
                     /gene="ectA"
                     /locus_tag="IE283_RS21715"
                     /old_locus_tag="GCM10010348_43930"
     CDS             2969..3481
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448158.1"
                     /locus_tag="IE283_RS21715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_43930"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_003976956.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPRVADGAALWRIARDSEVLDLNSSYSYLLWCRD
                     FAATSAVVRDGHGVPVGFITGYVRPDSPDTLLVWQVAVDGTYRGRGLAATLVDGLADRV
                     ARERGITILETTISPDNTASQRLFTSFAERRGARLEREVLFDTAVFPDGPHEPEVLYRI
                     GPLSAGG"
     gene            3611..4882
                     /gene="ectB"
                     /locus_tag="IE283_RS21720"
                     /old_locus_tag="GCM10010348_43940"
     CDS             3611..4882
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 1.5e-72.
                     Score: 236.4. Matches aSDomain:
                     nrpspksdomains_IE283_RS21720_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="IE283_RS21720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_43940"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_003976955.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-77, bitscore:
                     256.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAVGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNAVLKRALIDYLERDGVTHGLDMSTTAKRRFLETFQNTILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFIWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALADLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGMPMA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTATATLEAYWADGSAMEKQTRKRGEQVEQHM
                     IAITEENLADVKEYRGRGLVWGLEFHDKDRAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGMKTLARAVRETA"
     aSDomain        3698..4708
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE283_RS21720_Aminotran_3.1"
                     /evalue="1.50E-72"
                     /label="IE283_RS21720_Aminotran_3.1"
                     /locus_tag="IE283_RS21720"
                     /protein_end="366"
                     /protein_start="29"
                     /score="236.4"
                     /tool="antismash"
                     /translation="DRAVGSRMYDEDGHEYLDFFAGAGSLNYGHNNAVLKRALIDYLER
                     DGVTHGLDMSTTAKRRFLETFQNTILRPRDLPYKVMFPGPTGTNAVESALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFIWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALADLCERQDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTVSKSISGYGMPMALTLFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATATLEAYWADGSAMEKQTRKRGEQVEQHMIAITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5399
                     /locus_tag="IE283_RS21725"
                     /old_locus_tag="GCM10010348_43950"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="IE283_RS21725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_43950"
                     /product="ectoine synthase"
                     /protein_id="WP_003976954.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.6e-55, bitscore:
                     179.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEFEGTDRHVKSASGTWESTRIVLAKEKVGFSVHETILY
                     AGTETSMWYANHIEAVVCTKGDAELTDRETGKTYHITPGTMYLLDGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            5406..6305
                     /gene="thpD"
                     /locus_tag="IE283_RS21730"
                     /old_locus_tag="GCM10010348_43960"
     CDS             5406..6305
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="IE283_RS21730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_43960"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_003976953.1"
                     /sec_met_domain="PhyH (E-value: 8.9e-43, bitscore: 141.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTTNVTDLYPTRGATEVATPRQDPVVWGSPDAPGPVSAGDLQ
                     ALDRDGFLAIDQLITPDEVGEYQRELERLTTDPAIRADERSIVEPQSKEIRSVFEVHKI
                     SEVFAKLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTISVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEG
                     LTKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFA
                     APIRRPEFIGARDFTPVK"
     gene            complement(6387..7481)
                     /locus_tag="IE283_RS21735"
                     /old_locus_tag="GCM10010348_43970"
     CDS             complement(6387..7481)
                     /NRPS_PKS="Domain: Aminotran_5 (28-305). E-value: 5.1e-23.
                     Score: 73.5. Matches aSDomain:
                     nrpspksdomains_IE283_RS21735_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448151.1"
                     /locus_tag="IE283_RS21735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_43970"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_011028020.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.2e-19, bitscore:
                     63.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGEVTGPHSGAMESFENLVRTEFAPKTTYLNTASTGLLPARTLA
                     AMRDAAASAAEGQPLDMFADVEASRAAYARLVGVPATRVATGASVATFSGLVAASLPPG
                     AEVLTAEADFSSLVNPLYTRGDLKVRSVPLERLAESVGPDTALVAVSSVQSADGRLADL
                     AALRDAARAHGARTLIDASQSNGWLPLAAGDFDFVVAVAYKWVLCPRGVAFLVVPEDLG
                     AAAGLTPLFAGWASGEHPWDSCYGPVEELAHSARRFDESPALFSYTGARHSLALIEELG
                     VDAVHAHDTGLADRFRAGVRSLGHEPVDAPGSAIVSVPGLGHRQGELGGAGVQVSDRAG
                     NLRAAFHAYNTAADVDRLLDVLAG"
     aSDomain        complement(6567..7397)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE283_RS21735_Aminotran_5.1"
                     /evalue="5.10E-23"
                     /label="IE283_RS21735_Aminotran_5.1"
                     /locus_tag="IE283_RS21735"
                     /protein_end="305"
                     /protein_start="28"
                     /score="73.5"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARTLAAMRDAAASAAEGQPLDMFADVEASRAAY
                     ARLVGVPATRVATGASVATFSGLVAASLPPGAEVLTAEADFSSLVNPLYTRGDLKVRSV
                     PLERLAESVGPDTALVAVSSVQSADGRLADLAALRDAARAHGARTLIDASQSNGWLPLA
                     AGDFDFVVAVAYKWVLCPRGVAFLVVPEDLGAAAGLTPLFAGWASGEHPWDSCYGPVEE
                     LAHSARRFDESPALFSYTGARHSLALIEELGVDAVHAHDTGLADRFRAGVRSLGH"
     gene            complement(7541..8254)
                     /locus_tag="IE283_RS21740"
                     /old_locus_tag="GCM10010348_43980"
     CDS             complement(7541..8254)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976951.1"
                     /locus_tag="IE283_RS21740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_43980"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_003976951.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDGVEVVHRSFELDP
                     GRAKDDVQPVLTMLTAKYGMSQEQAQAGEDNLGAQAAAEGLAYRTRDRDHGSTFDLHRL
                     LHLAKERGRHEALLDAFYRGNFADERSVFNDDERLVELAVGAGLDAEEVRAVLADPAAY
                     ADEVRADEREAAQLGATGVPFFVLDRAYGVSGAQPAEVFTQALTQAWGERTPLKLIDDG
                     GAEACGPDGCAVPGH"
     gene            complement(8347..8955)
                     /locus_tag="IE283_RS21745"
                     /old_locus_tag="GCM10010348_43990"
     CDS             complement(8347..8955)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="IE283_RS21745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_43990"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_011028021.1"
                     /transl_table=11
                     /translation="MTLDLPELPFPLRPYGPDGRWSYADGVLTGVAGSRQDRFVPPTGE
                     SLESVSDAPRLLGAPDGDFQLIARVSVGFRGAFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDCNSFTVEGDSVWFRVSRTGRAFAFHASLDGERWTFVRLFTLGDEEE
                     TGSALVGFLAQAPTGEGCTVTYDHLEFRPAWPADLRDGS"
     misc_feature    8925..8938
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 17.76"
                     /tool="antismash"
ORIGIN
        1 cgtagcggcg ttcgtggagc cggcgcatgt gggccgacca gcgcgggaac cgggactcgc
       61 cgccgtcggc cagcgcgggg aaggtggcga tgttgaccag ggtggggacg atggcgacgc
      121 cgcgctcggc gaagagcggg atcgtgtcgt cggtcagtcc cgtggcgtgc tccacgcagt
      181 cgatgcctgc ctcgaccagg tcgcgcaggg agtcctcggc gaagcagtgc gcggtgacgc
      241 gggcgcccag ccggtgggcc tcggcgatgg ccgcctccac ggcgccgcgc ggccagcagg
      301 ccgacaggtc gccgaggtcg cggtcgatcc agtcgccgac cagtttcacc cagccgtcgc
      361 cgcgccgggc ctcctgggcg acgtaggcga cgaggtcgtc gggctcgacc tcccaggcgt
      421 agttgcggat gtagcggcgg gtgcgggcga tgtgccggcc ggcccggatg atcttcggga
      481 ggtcctcgcg gtcgtcgatc cagcgggtgt cggagggcga gcctgcgtcc cggacgagga
      541 gggtgcccgc ctcgcggtcg gtcagcgcct gcttctcggc cacgtccgcg gggacggcgc
      601 cgtgctggtc gaggccgacg tggcagtgcg cgtcgaccag gccgggcagg gcccatccct
      661 cgacggtgcg gacgtcggcg gcgcccgtgg ggcggtcgta ggagatccgt ccgtcgatca
      721 cccacacctc gtcccggacc tcctccggtc cgacgagcac ccgacccttc acgtgcagca
      781 ccgcgcgatc gctcatgccc cgcaccctaa acggtgcccg cacacccgta cggggccggt
      841 cacgggacgg gtcacccgcc tccgccgcgc gctgtcgcgc gggcgcgcgc ctcactcgcg
      901 tacccggccc cgacggcgcc cgcgacggcg gtcagggcca gggcggcggt ggcggggttg
      961 acgtagccgg ggacgtcgtc gctggtgtac tcgccgccgc cggtggtctc gcagacgaag
     1021 cccagcggca ggaaggagac cgactggtcg atgacgtgga gggcgcgctc ctcgttcccg
     1081 ggcgtgcggc agggcggcgc cggcgcggag cccgcgccgc cgtcggactc catgacggcg
     1141 ccggtcacgt gcagcagccc ccaggcgtac atcgcgacgg cggcggcacc ggcgaaggcg
     1201 gcgagggcgc gcagagccgg tccggcgccc gttccccggg cgcggcgggc ggcgaggacg
     1261 ctcgcgccgt ggccggtcag gccgacggcg gacaccacgg cgaccacgac gatcagcagg
     1321 agtacgagga acacgggccc agtccatcag gcggtgatct cttcggctgt gacggaagcc
     1381 acgattccgg gcgtgggggt gtcgcaacgg aagcactccg gcgcgagtgc cggcggacgc
     1441 catggttact ctcgactccg tacgccgcgt acccgcgccg cacacccgcc gtcaccgaag
     1501 agagcaccgc cgtgacccat cccttcctgg acctcccccc gctgagcgcc ggacgtttcg
     1561 ccgcgatcga ggaccgggtc gcgcgcctgc tcggcaccgg ccaggacgtg gtggtcatgc
     1621 agggcgaggc gctgctgccg ctggagggcg ccatccgcgc cgccgcgggg cccggcaccg
     1681 tcgcgctgaa cgtcgtcacg ggaccgtacg ggcagacctt cggcaactgg ttgcgggact
     1741 gcggcgcgac cgtgcacgac ctcgccgtgc cgttccacac ggccgtctcc gccgagcagg
     1801 tcggccgggc cctggccgag cacccggaga tcgacttcgt gtccctggtg cacgccgagg
     1861 cggcgaccgg caacaccaac ccggtcgcgg agatcggcga ggtggtccgg gcgcacggcg
     1921 cgctgttcta cctggacgcc gtggcctcgg tgggcgcgga gccggtgctg ccggacgcct
     1981 ggggcgtgga cctgtgcgtc atcggcgcgc agaaggccat gggcggcccg gcgggcgtct
     2041 cggcggtgtc ggtgagcgag cgggcctggg cccgcatggc ggcgaacccg aacgccccgc
     2101 ggcggtcgta cctgtcgctc ctcgactgga aggagcgctg gatcgacggc gggcgcaagg
     2161 cgctgctgca cgcgccggcg cagttggaga tgctcgcgct ggaggcctgc gtcgagcgca
     2221 tcgaggcgga gggccccggc acggtgatgg cgcggcacgc gtccgcggcg gcggccaccc
     2281 gggccggcgc gctcgcgctg ggcggcctgg agccgtacgt gtacgaggcg ggggacgcgg
     2341 cgccggtggc gacgacactg cgcacgccgt cggacgtcga cgcgtccgcg ctggtggccc
     2401 gtgccctgga gtccgacccg gcgctgccgc tggcggcggg cggcggcgcg ctggcgggcg
     2461 agatgatccg ggtgaaccac tacggtccgg acgcgacgcg cggggccgtg cagggctgcc
     2521 tggcggcact gggtgccgcg ctggtcgagc ggggcgtgaa ggccgacccg gaggccgccc
     2581 ggcgggccgc cgaggaggcg tgggagcggg cgaccgggcc cggcctttcg gaaggatgaa
     2641 tcggagggaa ttccggatac tcgcacaagc agaatttaag aattctactc agcgcagctt
     2701 cccgtattct tctgcccgct ttcttcggac ctaaacacaa agatttcacg actaacgatc
     2761 ttcacatttc ggacacgtct cgcacgggtt acgcgattgt gacccgatcc acatgctgcc
     2821 cgatttgcgg cttacgcacc cgtcaaatcg ccccataacg ccgtcgtctc gcgcggaagc
     2881 acgcgcccgc gtgataacac aagcggcact cattcgtaac cccatccggc gatgcaattt
     2941 cgaatttcgc tgggtaaatt caatttgcat gactgccgcg caagcagacc tgcaaatcga
     3001 tcgtccgaga gtggccgacg gcgccgccct gtggcggata gcgagggatt ccgaggtcct
     3061 cgacctgaac tcctcgtaca gctacctgct ctggtgccgg gacttcgccg ccacctcggc
     3121 cgtcgtccgc gacgggcacg gcgtgccggt cggcttcatc accggctacg tacggccgga
     3181 cagtcccgac accctgctcg tgtggcaggt ggcggtggac gggacgtacc gtggtcgtgg
     3241 gctcgcggca acgctggtcg acggactggc ggaccgggtg gcccgcgaac gcgggatcac
     3301 catcctggag acaacgatct ctcccgacaa cacggcgtcc caacgactgt tcacctcgtt
     3361 cgccgagcgc cgcggcgccc ggcttgagcg cgaagtcctg ttcgacacgg cggtgttccc
     3421 cgacgggccc cacgagcccg aggtcctgta ccgcatcggc cccctgtccg ccggcggctg
     3481 acccctcgcc gagccgtcgg ggcacccccg accgtccggc ccccagactt cccggccggg
     3541 accgcgaccg gtcccaccgg acccgtacga ccttcctctc ccaccctcac cccacgagga
     3601 gcgattcgtc gtgaccatca cccagcccga cctcagcgtc ttcgagaccg tcgagtccga
     3661 ggtgcgcagc tactgccgcg gctggcccac cgtcttcgac cgtgcggtcg gcagccgcat
     3721 gtacgacgag gacggccacg agtacctcga cttcttcgcc ggagcgggct cgctcaacta
     3781 cgggcacaac aacgcggtcc tgaaacgcgc gctgatcgac tacctggagc gggacggcgt
     3841 cacgcacggg ctcgacatgt cgacgacggc caagcgccgc ttcctggaga ccttccagaa
     3901 caccatcctg cggccccgcg acctgcccta caaggtcatg ttcccgggcc cgacgggcac
     3961 caacgccgtc gagtccgcgc tcaagctggc gcgcaaggtg aaggggcgcg agtcgatcgt
     4021 gtccttcacc aacgccttcc acggcatgtc gctgggctcc ctcgcggtga ccggcaacgc
     4081 cttcaagcgg gccggcgccg gcatcccgct ggtgcacggc acgcccatgc cgttcgacaa
     4141 ctacttcgac ggcaccgtcg aggacttcat ctggttcgag cgcctgctcg aggaccaggg
     4201 ctccggcctg aacaagccgg ccgccgtgat cgtcgagacc gtgcagggcg agggcggcat
     4261 caacgtcgcc cgcgccgagt ggctgcgcgc cctggccgac ctgtgcgagc gccaggacat
     4321 gctgctcatc gtcgacgaca tccagatggg ctgcggccgt accggcgcct tcttctcctt
     4381 cgaggaggcg ggcatcacgc cggacatcgt caccgtctcc aagtccatca gcggctacgg
     4441 catgccgatg gcgctgaccc tgttcaagcc ggagctggac gtctgggagc cgggcgagca
     4501 caacggcacc ttccgcggca acaacccggc cttcgtcacg gcgaccgcca ccctggaggc
     4561 gtactgggcc gacgggtcgg cgatggagaa gcagacccgc aagcgcggcg agcaggtcga
     4621 gcagcacatg atcgccatca ccgaggagaa cctcgccgac gtcaaggagt accgcggccg
     4681 cggcctggtc tggggcctgg agttccacga caaggaccgc gcgggccggg tcgccaagcg
     4741 cgccttcgag ctggggctgc tcatcgagac ctccggcccc gagagcgagg tcgtcaagct
     4801 gctgccggcg ctcaccatca cgcccgacga gctggacgag ggcatgaaga ccctggccag
     4861 ggccgtgcgc gagaccgcgt gacggtctga cggcccggcc gtcgggcacc accaccacga
     4921 actcccggcg tcttcggtcc gcgcggccga ggacgccggg gccccttgca cacccttcac
     4981 ctaggaggca tcgcaaaacc gtgatcgtcc gttcgttcaa ggagttcgaa ggcaccgacc
     5041 ggcacgtgaa gagcgcgtcc ggcacctggg aaagcacccg gatcgtcctc gccaaggaga
     5101 aggtcggctt ctccgtgcac gagacgatcc tgtacgcggg tacggagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtcgtctgca ccaagggcga cgccgagttg accgaccgcg
     5221 agacggggaa gacgtaccac atcacgccgg gcacgatgta tctcctcgac ggccacgagc
     5281 ggcacacgct caaggtcaag gaggacttcc actgcatctg tgtcttcaac ccgcccgtca
     5341 ccggacggga ggatcacgac gagaacggcg tctacccgct gctcaccgag gaggtgtgag
     5401 tcaccgtgac caccacgacc acgaacgtca ctgatctcta tcccacccgc ggcgccaccg
     5461 aagtggccac ccctcggcag gacccggtcg tctggggctc cccggacgcg cccggccccg
     5521 tctctgcggg cgacctccag gcgctggacc gcgacggctt cctcgccatc gaccagctga
     5581 tcaccccgga cgaggtcggc gagtaccagc gtgagctgga gcgcctcacc accgacccgg
     5641 ccatccgcgc ggacgagcgc tcgatcgtcg agccgcagtc caaggagatc cggtcggtct
     5701 tcgaggtgca caagatcagt gaggtcttcg ccaagctggt gcgcgacgag cgcgtggtcg
     5761 ggcgggcgcg gcagatcctc ggctcggacg tgtacgtcca ccagtcgcgg atcaacgtca
     5821 agccgggctt cggggccagc ggtttctact ggcactcgga cttcgagacc tggcacgccg
     5881 aggacggcct gcccaacatg cgcacgatct cggtctcgat cgcgctcacc gagaactacg
     5941 acaccaacgg cggtctgatg atcatgccgg ggtcgcacaa gacgttcctc gggtgcgcgg
     6001 gggccacgcc gaaggacaac tacaagaagt ccctgcagat gcaggacgcg gggacgccgt
     6061 ccgacgaggg cctgacgaag atggcctcgg agtacggcat caagctgttc accggcaagg
     6121 ccggttcggc gacctggttc gactgcaact gcatgcacgg gtcgggcgac aacatcacgc
     6181 cgttcccgcg cagcaacgtg ttcatcgtgt tcaacagcgt ggagaacacg gcggtcgagc
     6241 cgttcgcggc tcccatccgg cggccggagt tcatcggggc gcgggacttc acgccggtga
     6301 agtgactcgc gtctgagact cgggcagggg ccttctagtg tgggacggga ggcccctgcc
     6361 cgatgtttct tcccggcccg gccgggtcag cccgccagca cgtcgagcag ccggtccacg
     6421 tcggccgcgg tgttgtacgc gtggaaggcg gcccgcagat tgcccgcccg gtccgagacc
     6481 tggacgccgg ccccgcccag ctcgccctgc cggtgtccga gtcccggcac ggacacgatc
     6541 gcggagcccg gcgcgtccac cggctcgtgg cccagcgagc gcaccccggc gcggaaccgg
     6601 tcggcgagcc cggtgtcgtg ggcgtgcacc gcgtcgacgc ccagttcctc gatcagggcc
     6661 agggagtgcc gggcgccggt gtaggagaac agggcggggc tctcgtcgaa ccgccgggcg
     6721 gagtgcgcga gttcctccac ggggccgtag cagctgtccc aggggtgctc accggacgcc
     6781 cacccggcga acagcggtgt cagcccggcc gccgccccca ggtcctccgg tacgacgagg
     6841 aaggcgacgc cgcgcggaca caggacccac ttgtaggcga cggcgaccac gaagtcgaag
     6901 tcgccggcgg ccagcggcag ccacccgttc gactgcgagg cgtcgatcag cgtccgcgcc
     6961 ccgtgcgccc gggcggcgtc ccgcagcgcg gcgaggtcgg cgagccgccc gtcggcggac
     7021 tgcacggaac tgaccgcgac gagcgcggtg tcaggaccca ccgactcggc cagccgttcc
     7081 agcggcacac tgcgcacctt caggtcgccg cgcgtgtaca gcgggttcac cagggagctg
     7141 aagtcggcct ccgcggtgag gacttcggcg cccgggggca gcgacgcggc gacgagcccg
     7201 ctgaacgtgg cgacggacgc gccggtcgcg acccgggtgg cgggcactcc cacgaggcgg
     7261 gcgtacgcgg cccgggacgc ctcgacgtcg gcgaacatgt cgagcggctg cccctccgcg
     7321 gcggaggccg ccgcgtcgcg catggccgcg agggtccggg cggggagcag gccggtgctg
     7381 gcggtgttca gatacgtggt cttcggggcg aactcggtgc ggacgaggtt ctcgaagctc
     7441 tccatggcac cactgtgcgg ccccgtgacc tccccgtcca ttgcgtattt ctacgcgata
     7501 cccccaaggg acacttatga gtccgtcccg acctgccgat tcagtggccg gggaccgcgc
     7561 acccgtccgg gccgcaggcc tcggcgccgc cgtcgtcgat cagcttcagc ggcgtgcgct
     7621 cgccccacgc ctgggtcagt gcctgggtga agacctcggc cggctgggcg cccgagactc
     7681 cgtaggcccg gtcgagcacg aagaacggca ccccggtggc cccgagctgg gcggcctcgc
     7741 gctcgtcggc gcgcacctcg tcggcgtagg cggcggggtc ggcgagcacc gcgcggacct
     7801 cctcggcgtc cagcccggcg ccgacggcca gctccacgag ccgctcgtcg tcgttgaaca
     7861 cggagcgctc gtcggcgaag ttgccccggt agaaggcgtc gaggagcgcc tcgtgccgtc
     7921 cccgctcctt ggcgaggtgc agcaggcggt gcaggtcgaa ggtgctgccg tggtcgcggt
     7981 cccgggtgcg gtaggccagg ccctcggcgg cggcctgcgc gccgaggttg tcctcaccgg
     8041 cctgcgcctg ctcctggctc atgccgtact tggcggtgag catcgtcagc accggctgca
     8101 cgtcgtcctt ggcccgtccg gggtccagtt cgaacgaacg gtggaccacc tcgacgccgt
     8161 cacggtgcgg gaaggccgcg agcgccttct cgaagcgggc cttgcccacg tagcaccagg
     8221 ggcaggcgat gtcgctccag atctcgacgc gcatgtctcg gctcttctcc aggtcgtacg
     8281 gcggcggagg ccctctccgc cccgtacgtg aacgttcaag cagccgttct cattcccgcc
     8341 ccgcgctcag ctgccgtccc gcagatccgc gggccaggcc ggccggaact ccaggtggtc
     8401 gtaggtcacg gtgcagccct cccccgtcgg cgcctgcgcc aggaagccga ccagggcgga
     8461 gccggtctcc tcctcgtcgc ccagtgtgaa gagccgcacg aaggtccagc gctccccgtc
     8521 gagggaggcg tggaaggcga aggcccgtcc ggtccggctg acccggaacc agacggagtc
     8581 gccctccacg gtgaaggagt tgcagtcgtc ggagtgcccc cgggtgacca ccgtgcagac
     8641 ggtgggcacg tccggggagt actccagaca cagcttggcc caggcccgct cccccacgtg
     8701 cacgtagagc accccggcgt cgaaggcccc gcggaacccg acggacacac gggcgatcag
     8761 ctggaagtcc ccgtcgggcg cccccagcaa ccgcggcgcg tcggacacgg actccagcga
     8821 ctccccggtg ggcggcacga accggtcctg ccgggacccg gcgaccccgg tgagcacacc
     8881 gtcggcgtag gaccagcggc cgtcgggccc gtagggacgg aggggaaacg gcagttcggg
     8941 aaggtcaaga gtcatgcact gatcatcgca ggcggaggga agtcccctga ggggcgccgg
     9001 gctgtgtcga tctgcggctc cgccgcgcgg gcgcgacaag ccacgacggc gcgtgagccg
     9061 ccgaacaacc cgcgccccta cggcgcaacc gcggagcgct accgctcgag cactccgttg
     9121 aaccgccgcg gcagcccctg cggattgtcg tcccggagct ccaccggcag cagcgcctcc
     9181 ggcaccccct gatacgccac cggccggagc caccgctcga tcgccgtacc ccccaccgac
     9241 gtggacgtcg aagtggtcgc cgggtaaggc cccccatgct gctgagccgc cgccacggca
     9301 acccccgtgg gccagccgtt gaccagcacc cgccccgcca acggcgtcac ctccgcgagg
     9361 agagccgccc cggcaccctc tcccgccact tcctcggcag agagctgcac cgtcgccgtg
     9421 aggttccccg gcagtcgcga cagcaccccc cggacctccc cctcgtcctg atagcgggcc
     9481 acgacggtga ccggcccgaa gcactcctcc aggagcaggt cgtgctcgcc ctccgccgcc
     9541 agccgcgccg ccggcaccgt gaggaacccg gcgctgacgg tgtgctcgcc gcccgagccc
     9601 ggcgtcaccg gggagtccac gtcgggcagc gcggcgcgct cggcgacgcc tgcgacgaag
     9661 ttgtcccgca tgcggtggtc gaggagcaca ccggcgtcgg tggcgctgac cgcgtcggtg
     9721 agcgacttga ccaggccgtc gccggcgtcg cccgccggca cgagcaccag gccgggcttg
     9781 acgcagaact gcccgacgcc cagcgtcatg gagcccgcca ggcccgcgcc gatcgcctcc
     9841 gcccgctcca cggccgccgc ctccgtgacg acgaccgggt tcagcgagcc cagctcgccg
     9901 tggaacggga tcggcaccgg acgcgccgcc gccgcgtcga agagcgcgcg gccaccgcgg
     9961 acggagcccg tgaagcccgc cgcggcgacc agcgggtgct tgatcagctc gacgcccgcc
    10021 tcgaagccgt gcaccaggcc gaggaccccg tccgggatgc cgtgccgggc cgcggcccgg
    10081 cgcagcacct tggcgaccag ctcggacagg gccgggtggt cggggtgcgc cttgacgacg
    10141 accgggcagc cggccgcgag cgcgctcgcg gtgtcgccgc cggggacgga gaaggcgaag
    10201 gggaagttgg aggccgagta gacggcgacg acgccgagcg gcaccttgta gcggcgcagg
    10261 tcggggatgg gcggggtggc cgtgtcgtcg gggtggttga cgaccacgcc gaggtactcg
    10321 ccctcgtcga cgatgtcggc gaaggcccgc agctggtagc aggtgcgggc gagttcgccg
    10381 gtgagccgga ccgggccga
//
