LOCUS       NZ_BMVQ01000002        10570 bp    DNA     linear   CON 11-FEB-2024
DEFINITION  Streptomyces anthocyanicus strain JCM 4739 sequence02, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVQ01000002
VERSION     NZ_BMVQ01000002.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces anthocyanicus
  ORGANISM  Streptomyces anthocyanicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces violaceoruber group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 772717)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:14:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,934
            CDSs (total)                      :: 7,858
            Genes (coding)                    :: 7,681
            CDSs (with protein)               :: 7,681
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 73 of 177
            Pseudo Genes (incomplete)         :: 130 of 177
            Pseudo Genes (internal stop)      :: 16 of 177
            Pseudo Genes (multiple problems)  :: 37 of 177
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:48:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 354794
            Orig. end                         :: 365364
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVQ01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10570
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10570
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10570
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5570](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5570
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(969..2279)
                     /locus_tag="IE283_RS07990"
                     /old_locus_tag="GCM10010348_16350"
     CDS             complement(969..2279)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976094.1"
                     /locus_tag="IE283_RS07990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16350"
                     /product="glycosyltransferase"
                     /protein_id="WP_193484963.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.4e-32,
                     bitscore: 108.0, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLPPTGLRPRVLHLTQPVDGGVARVVADLTRAQLAAGLRVAVAC
                     PAGGLADRLRALGADVRHWPSTRSPGPALVEEVRRLVRVVEDVRPDLVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEAVGGAAALLALGWERWAARWTARTVCVSEAERLRGTRAG
                     LRGPWTVIPNGIDLAGPSAGPSADAPGEAPADTSDDAAGVRRLHGVGPHAPLVVCVGRL
                     CRQKGQDVLLSAWESVLARVPGARLVLVGDGPDRARLTARAPASVLFTGTVADAAPWYR
                     AADLVVLPSRWEGMALAPLEAMACGRPVVVTDVDGAREGLPPALVPHCVVPPEDPAALA
                     DAAAALLLDAPLRASLGRRGRAHVRSAHDVRHTAAAVAALYGDLLYGDVLDGLRPPPPA
                     HTGDGDRFGDIGDIGDRPADVRRPAVVPIECRESTYP"
     misc_feature    complement(2256..2258)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2533..2772
                     /gene="chpG"
                     /locus_tag="IE283_RS07995"
                     /old_locus_tag="GCM10010348_16360"
     CDS             2533..2772
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="IE283_RS07995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16360"
                     /product="chaplin ChpG"
                     /protein_id="WP_007449696.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVAGTAGVAAADSGAQAAAAHSPGVLSGN
                     VVQVPVHIPVNVCGNTIDVIGLLNPAFGNECEND"
     gene            3048..3782
                     /locus_tag="IE283_RS08000"
                     /old_locus_tag="GCM10010348_16370"
     CDS             3048..3782
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976096.1"
                     /locus_tag="IE283_RS08000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16370"
                     /product="hypothetical protein"
                     /protein_id="WP_003976096.1"
                     /transl_table=11
                     /translation="MRLPARRIATSALCAGLLIGISGPAVMAADGDSVRERTHAASRAP
                     LPDAEELQSQVDSLAGLGDVLTPVTDLLGAVLKADNGQLSATDADKLSAAVKDALAKAD
                     AADTDADDAATTPGTTTPAQPPAVTAPEAGTDTPVTLPAPVTAQSDDGTAAASDLTAAA
                     NAALQKQIDALVKATTSGTAEQVTPAVKDLMTGIVNVVAATMVGNELPAADLAGLPGVP
                     TAPEATTPEAATPEAAAPANPS"
     gene            3959..4261
                     /locus_tag="IE283_RS08005"
                     /old_locus_tag="GCM10010348_16380"
     CDS             3959..4261
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445910.1"
                     /locus_tag="IE283_RS08005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16380"
                     /product="hypothetical protein"
                     /protein_id="WP_193484965.1"
                     /transl_table=11
                     /translation="MKSLKAAVVVAGSLVAAGSAVPAFAYDASDAAALTPTSLNGAVNA
                     LTAEPIDVMPIKHQSEARSDALDTENKDSVLHTVKGATTKLNSAKGLLGGLPLGG"
     gene            4366..4881
                     /locus_tag="IE283_RS08010"
                     /old_locus_tag="GCM10010348_16390"
     CDS             4366..4881
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="IE283_RS08010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16390"
                     /product="hypothetical protein"
                     /protein_id="WP_003976098.1"
                     /transl_table=11
                     /translation="MKPTTRRTLAAVVTGVAAAVGVAATPAAAARAVPVPVPLGGAEAA
                     LGMELPEVAGELPVPTAGAPEGPRYVEGRLLPERTLPQLPVRAGLPGVDVRQPLPHVLG
                     DDFDHAGVDAPAAGLRTLAPGLSLDAPLTAPNPEALGLPSPKLPEVGVLAPVVQTVPVA
                     DLGVGPGL"
     gene            5001..5570
                     /locus_tag="IE283_RS08015"
                     /old_locus_tag="GCM10010348_16400"
     CDS             5001..5570
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028525.1"
                     /locus_tag="IE283_RS08015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16400"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_193484967.1"
                     /sec_met_domain="melC (E-value: 9.2e-53, bitscore: 173.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVGNAGATANGAEREYATEDAARGGPASGTRRQVMRGLLAPALAV
                     GLAPLIAASRPSGAAEAATTGPLPAHPGPHAAESAESVDGAAEGVEGAAFDETYRGRRI
                     RGIRSAAGRAVGAGTWQVTVDGRPLHLMRRADGSWLSMVDHYRSYPTPLAAARGAVDEL
                     GPGEHLRDTPSTDHGRHSGGRHGVHA"
     gene            5554..6420
                     /locus_tag="IE283_RS08020"
                     /old_locus_tag="GCM10010348_16410"
     CDS             5554..6420
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="IE283_RS08020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16410"
                     /product="tyrosinase family protein"
                     /protein_id="WP_193484968.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRTEKRRFVNALLEIKRRGEYDEFVRTHIEYYVS
                     DGENGLRTAHMAPSFLPWHRRFLLDLEEALRRVDPSVTVPYWDWTKDRSAKSAPWTADL
                     LGGTGRRSDHRVTTGPFAHAGGNWTIKVNVTDTQYLTRDLGRAADPLGLPTKSDLEWAL
                     DDPKYDTSPYDSTVRKGFRNKLEGWGTGRGSVSWRNHNRVHRWVGGAMVGGASVNDPVF
                     WLHHAFIDLQWSRWQARHRGARYLPAEPPGRGSAQRGRIVARHEKLPPWDVTPDELEDV
                     GRIYRYA"
     gene            complement(6512..6784)
                     /gene="chpF"
                     /locus_tag="IE283_RS08025"
                     /old_locus_tag="GCM10010348_16420"
     CDS             complement(6512..6784)
                     /codon_start=1
                     /gene="chpF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028524.1"
                     /locus_tag="IE283_RS08025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16420"
                     /product="chaplin ChpF"
                     /protein_id="WP_011028524.1"
                     /transl_table=11
                     /translation="MSRIAKAAGVALGTGAVVLSGTGMAMADAGAAGAAVGSPGVLSGN
                     VVQVPVHVPVNLCGNTIDVIGLLNPAFGNACENGDDDDKSGGYGG"
     gene            complement(6983..7111)
                     /locus_tag="IE283_RS40195"
                     /old_locus_tag="GCM10010348_16430"
     CDS             complement(6983..7111)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE283_RS40195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010348_16430"
                     /product="hypothetical protein"
                     /protein_id="WP_281401790.1"
                     /transl_table=11
                     /translation="MDDVDDMDDMDEDCEVSEALEALEAPEVSMDESARSFGMPGG"
     gene            7110..7688
                     /locus_tag="IE283_RS08030"
                     /old_locus_tag="GCM10010348_16440"
     CDS             7110..7688
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976102.1"
                     /locus_tag="IE283_RS08030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16440"
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_193485130.1"
                     /transl_table=11
                     /translation="MASALAALPALFAEESRVRAFAAVALGAGDAAQVAGAAGLSAKEA
                     AGALRRLREQGVVTSGDDGGLVVAHGRFRELARVGRTESAAPRDAAAAGDGGPGDMIVR
                     TFVRNGRLVRLPARWTRKKVVLRHIAEQTFAPGVEYPERTVDEKLRAWCEDGEVDHVTL
                     RRHLVDFGHLRRSDGVYARPADSARTGTA"
     gene            complement(7695..8687)
                     /locus_tag="IE283_RS08035"
                     /old_locus_tag="GCM10010348_16450"
     CDS             complement(7695..8687)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028521.1"
                     /locus_tag="IE283_RS08035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16450"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_193484971.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.3e-52, bitscore:
                     173.1, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDYQGVATEAADWSPVADRVEVVSHGTHFPDEDALAA
                     ALADADIVVTLRERVPFPGSLIARLPRLKLLVASGMRNSVIDYAAADAHGVTVCGTASS
                     STPPVELTWALILGLARGIVQEANGLREGGPWQQTVGADLHGRRLGLLGLGKIGGRVAQ
                     VGLAFGMRVSAWSQNLTRERTDEVGVELASSKEELLRTADFVSVHLALGERTRGLLGPA
                     ELALLKPTAYLVNTSRAAIVDQEALLTALHEGRIAGAGVDVFDTEPLPAGHPMRTAPRL
                     LATPHLGYVSRANYATYYGQAVEAIGAYLAGSPIRRLPVGSPPPAPSRA"
     gene            complement(8744..9514)
                     /locus_tag="IE283_RS08040"
                     /old_locus_tag="GCM10010348_16460"
     CDS             complement(8744..9514)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1226: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028520.1"
                     /locus_tag="IE283_RS08040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16460"
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="WP_011028520.1"
                     /transl_table=11
                     /translation="MRIEAVDWVEIPAGVVRRGTPADDIPLVAERYADTGVPVEWYLKE
                     APSTEIRLPAFRLARTPVTVGQWSVFAAATGRPVPTAPHDHPVTGVSWEAASAYCRWLG
                     ELSGDPGIRLPTEDEWERAARGDDGREFPWGDEYRTGLANLVDLGVGTTTPVGSFPDGA
                     GPFGVLDMAGNVDEWTSTLYAPYPGAPAGVPLVEDWAFDPHITRGGAFRHDRDLARCAR
                     RHSAYEEDLAAIGVGLRLAAPAAGTSSASASASA"
     misc_feature    9607..9620
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            complement(9635..10177)
                     /locus_tag="IE283_RS08045"
                     /old_locus_tag="GCM10010348_16470"
     CDS             complement(9635..10177)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976105.1"
                     /locus_tag="IE283_RS08045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010348_16470"
                     /product="hypothetical protein"
                     /protein_id="WP_003976105.1"
                     /transl_table=11
                     /translation="MSPPLITGLHINAVALGDSCRICAIWRVSRPAGPRPACEPVFALL
                     LPRRGTEQDWAAETALRALGEQRLGDGPVCVDLATTVDGIAVDTLRPGLCEPGLLEASR
                     AALGDGHQEWAELARGDPSAFHAAASDGYVLLRHAAVVTECDPAGPGGRVPVVHAREHR
                     VLRAYGAALLAHAGAGS"
ORIGIN
        1 cagcaccgcc gcgcaggcca gcagcagggg gctcagcagc agcaacagca gccccgacac
       61 cgtcacgtcg aggacccgct tgcccgcgcc gccggggcgc cgggccgccg gttccaggcg
      121 ccggcagggg aacccggcga tccggtcccc ggccgggcgg gccggggcgt cggcgtgttc
      181 gggcgcctcg gcgtccagct cccacaccgt gcaccccgac tcggccagcg tccgcagcag
      241 cgccgcctgc cggggccgtg cggaggggtt caccggggaa ccggcgacca ggacgtcccg
      301 tacgccgttc tgtacgaccg cccgccgcac ctcctcgtcc gtggacagca ccggcaggcc
      361 cgtgccgccg cccgggccgc cggtgacgat gccgaccggg cgcagcccgc agcgcgggtg
      421 gcgcagcagc gccgaggcca cgcgctgcgc cgtcgcggcg ggaccgacga ccagggcggc
      481 gcgggggcgg ttcaggagcg ccgcgcggtg tctgcggtgc accgcgcccc ggccggcgca
      541 gcccgtcacc gcgtgcagca ggaaaccggc gcacaggatg cgcgcggaca gcgcgtgcgc
      601 gggggcgtac gccgcgagga gcgccgcgag caccagccac accaccgcga tccggccgca
      661 cagggcgggc aggtcgtcca gcgcgccgga ggtccgcctg gccgggcgcg ggctcagcag
      721 gagcgaggcg gcgaccagca gggcggtgag cagaggttgg tgcggggtgc cggtgaggac
      781 cagcgagccc ggcagggccg ccgtgccgtc cgcgacgagc agcgccggcc ggaagtcccg
      841 ccgaggtgcg ggacgccggt cgggcagccg cgggcccgcg gtgccgtcgc gcggcggcag
      901 caccgagacg ggggaggagc cgaggtcgcg cgaccggccg gcgggggagg ggacggtgct
      961 ttccgcgctc acgggtaggt ggactccctg cactcgatgg gcacgacggc cggccgccgc
     1021 acgtccgcgg gcctgtcgcc gatgtcgccg atgtcgccga agcggtcgcc gtcgccggtg
     1081 tgcgccgggg gtgggggacg taggccgtcc aggacgtcgc cgtacaagag atcgccgtac
     1141 agggccgcca ccgcggcggc cgtgtgccgt acgtcgtgcg cggaccgcac gtgcgcccgc
     1201 ccccggcggc cgagcgacgc ccgcagcggc gcgtcgagca gcagtgcggc ggcggcgtcg
     1261 gccagcgccg ccgggtcctc gggcggcacc acacagtgcg gtacgagagc gggcggcagg
     1321 ccctcgcggg caccgtccac gtcggtcacc accacgggcc gcccgcaggc catcgcctcc
     1381 agcggggcca gtgccatgcc ctcccagcgc gacggcagga cgaccaggtc ggcggcccgg
     1441 taccaagggg ccgcatcggc gacggtcccg gtgaacagca cggacgccgg ggcccgcgcg
     1501 gtcagccggg cgcggtccgg gccgtcgccc acgaggacca gccgggcgcc cggcacccgg
     1561 gcgagcacgg actcccaggc gctcagcagg acgtcctggc ccttctgccg gcacagccgg
     1621 cccacgcaga ccaccagcgg cgcgtggggg ccgacgccgt ggagccgccg cacaccggcc
     1681 gcgtcgtcgg aggtgtcggc gggggcctcg ccgggggcgt cggcggacgg gccggcggac
     1741 gggccggcga ggtcgatgcc gttcgggatc accgtccagg gcccgcgcag cccggcccgg
     1801 gtcccccgca gccgttccgc ctcgctcacg cacaccgtcc gcgccgtcca gcgcgccgcc
     1861 caccgctccc agccgagcgc gaggagcgcg gcggccccgc cgaccgcctc gaacgaccag
     1921 gcgtgcggct ggaacacggt cgggatccgg ccccgcacgg cgagccgtcc ggccagaccg
     1981 gccttggcgc tgtgcgcgtg caccagatcg ggccgtacgt cctcgaccac gcgcacgagc
     2041 cgccgtacct cctccaccag cgccgggccg ggcgagcggg tcgacggcca gtgccgcaca
     2101 tcggcgccca gcgcacgcag ccggtcggcg agacccccgg cggggcaggc gaccgcgacg
     2161 cgcagtccgg cggcgagctg agcccgcgtc aggtccgcca ccacccgggc gaccccgccg
     2221 tccaccggtt gggtgaggtg cagaacccgt ggccgtaagc cggtcggtgg caggtgcatg
     2281 cgcgtttcct cgccatacgg gtgcgtcgaa cgggtgggtc gaacgggtgc gtcgaacggg
     2341 tgcgtcgaat ggtgtgggac tctagctgct atccgtacta atacggcgaa atcgggtaag
     2401 tgtgttggat ttgtgaacgc cggtatttcg ccagatcacc cctttggcgg cacaactcgc
     2461 gcccgtgcca cgcgttgaca ctgcgccggg cagccgcccg gatgtgtgta caaccccaag
     2521 gagcacttct ccatgtcgcg tatcgcgaag ggcctggccc tgacctccgt cgccgccgcc
     2581 gcggtggcgg gcaccgccgg tgtcgccgcc gccgacagcg gcgcgcaggc cgccgccgcc
     2641 cactccccgg gcgtcctgtc cggcaacgtg gtgcaggtcc cggtccacat cccggtcaac
     2701 gtctgtggca acaccatcga cgtcatcggc ctgctgaacc cggcgttcgg caacgagtgc
     2761 gagaacgact gacgtcggct caccacttgc cgcaccggcc gtccgtaccg cggttcctcc
     2821 gcgagcgggc ggccggttcg cgttgccgtc cggacggcgg tgtgctgccc cgaatggggg
     2881 gaccgggccg cgacacggcg cacggacctt gacagggcgt ctcaccaggt aagacgcggc
     2941 ccctgggaag ggcgggcggg cgccccgggc cgccgcctgc gttgcagagc gccgagggcg
     3001 cttccacggt gggcacaaca tccgacgcac caccgaaagg aacccccatg cgtctgcccg
     3061 cacggcgaat cgccacctcc gctctctgcg ccggcctgct gatcggtatc tccggtccgg
     3121 cggtgatggc ggccgacggc gactcggtcc gggaacgcac ccacgcggcg tcccgcgcac
     3181 ccctccccga cgccgaggaa ctgcagagcc aggtcgacag cctggccggt ctgggcgacg
     3241 tgctcacgcc cgtcaccgac ctgctcggcg ccgtcctcaa ggccgacaac ggccagctct
     3301 ccgccacgga cgccgacaag ctctccgccg ccgtcaagga cgccctcgcc aaggcggacg
     3361 cggcggacac ggacgccgac gacgcggcga ccacccccgg cacgaccacc ccggcccagc
     3421 cgccggccgt gaccgcgccg gaggccggga ccgacacccc ggtcacgctc cccgcgcccg
     3481 tcacggcgca gagcgacgac ggcacggcgg cggcgtccga cctgaccgcg gccgcgaacg
     3541 ccgcgctgca gaagcagatc gacgccctgg tcaaggccac cacctccggt accgccgagc
     3601 aggtgacccc ggccgtcaag gacctgatga ccggcatcgt caacgtcgtc gccgccacca
     3661 tggtcggcaa cgagctgccc gccgcggacc tggccggcct gcccggcgta ccgaccgcgc
     3721 cggaggccac gacgccggag gcggccactc cggaggccgc cgcaccggcg aacccctcct
     3781 gacgctccca cggcgttccg ggccgtcgcg cctccccctt tcggaggtgc ggcggccctt
     3841 tccgtgcccg gagatctcat atgtcttccg gacggcggag tttccgggcc cggcgcaggt
     3901 cgttagggag ggcgtcagca cacctccggt gacacgggtc gccgaagaaa ggaacaccat
     3961 gaagtccctc aaggccgccg tcgtcgtcgc cggttccctg gtcgccgccg gctccgccgt
     4021 gcccgcgttc gcctacgacg cctccgacgc cgccgcgctc acgcccacca gcctcaacgg
     4081 cgccgtgaac gcgctgaccg cggagcccat cgacgtcatg ccgatcaagc accagtcgga
     4141 agcgcggtcg gacgcgctgg acaccgagaa caaggactcc gtgctgcaca ccgtgaaggg
     4201 cgccaccacg aagctgaact ccgcgaaggg cctgctgggc ggccttccgc tgggcggctg
     4261 aaccgccccg ctcccgctcc ccgtcctgct tccgccttcc ggagccgtac ctcccgcctt
     4321 cccgaagccg aaccccccgc cttctcgaaa gggaccgtgc ctgtcatgaa gccgaccacc
     4381 agacgaaccc tggccgccgt ggtcaccggt gtggccgccg ccgtcggcgt agccgcgacc
     4441 cccgccgccg cggcccgcgc ggttcccgtg ccggtgccgc tgggcggcgc ggaggcggcc
     4501 ctcggcatgg agctgcccga ggtggccggc gagctgcccg tgccgacggc gggcgcgccg
     4561 gagggcccgc ggtacgtcga gggccggctg ctgccggagc ggacgctgcc gcaactgccg
     4621 gtgcgcgccg ggctgcccgg cgtcgacgtg cgccagccgc tgccgcacgt cctcggcgac
     4681 gacttcgacc acgccggtgt cgacgccccc gccgccgggc tgcgcaccct ggcgccggga
     4741 ctgtccctgg acgccccgct caccgcgccg aaccccgagg ccctcggcct gccgtcgccc
     4801 aagcttcccg aggtcggcgt gctcgccccg gtggtccaga cggttccggt ggccgacctg
     4861 ggcgtcggac cgggcctgta accctccggc gacctcgctc cccggcgcgg tcgtgctccg
     4921 agtggccctg cgggcggtga ccgcggggcc cgccgttcgg ttagcggtgc ggaccaaccg
     4981 aaccggaaga ggagtgagcc atggtcggca acgcgggcgc gacggcgaac ggcgccgagc
     5041 gggagtacgc gacggaggac gcggcgagag gcgggccggc gtccggcacc cgacggcagg
     5101 tgatgcgggg gctgctcgcc ccggccctcg ccgtcggcct ggcgcccctg atcgccgcct
     5161 cgcgaccctc cggggccgcg gaggccgcca ccaccggacc gctgcccgcc catcccgggc
     5221 cgcacgccgc cgagtccgcc gagtccgttg acggggcagc cgagggggtc gagggcgccg
     5281 ccttcgacga gacctaccgc ggccggcgca tccggggcat ccgctcggcc gcggggcggg
     5341 cggtgggcgc cggtacctgg caggtcaccg tggacggccg gccgctgcac ctgatgcgcc
     5401 gggcggacgg cagctggctg agcatggtcg accactaccg gtcgtacccc accccgctcg
     5461 ccgcggcccg cggcgccgtc gacgaactcg gccccggcga gcacctgcgc gacacgccgt
     5521 ccacggacca cggccggcac tccggggggc ggcatggcgt acacgcgtaa ggacgtcagc
     5581 acgctgaccc gcaccgagaa gcggcggttc gtcaacgcgc tgctggagat caaacgacgc
     5641 ggggagtacg acgagttcgt gcgcacccac atcgagtact acgtctccga cggcgagaac
     5701 ggcctgcgta cggcgcacat ggcgccgtcc ttcctgccct ggcaccgccg gttcctgctg
     5761 gacctggagg aggcgctgcg ccgggtcgac ccgtcggtca cggtgccgta ctgggactgg
     5821 acgaaggacc gcagcgccaa gtcggcgccc tggaccgcag acctcctcgg cggcaccggg
     5881 cggcggtccg accaccgggt gaccaccggc cccttcgccc acgccggggg gaactggacc
     5941 atcaaggtga acgtcaccga cacccagtac ctcacccggg acctcggacg tgccgccgac
     6001 cccctcggcc tgcccaccaa gagcgacctg gagtgggcgc tggacgaccc gaagtacgac
     6061 acctcgccct acgactccac ggtccgcaag gggttccgca acaagctgga ggggtggggg
     6121 acgggccgcg gcagcgtgtc ctggcgcaac cacaaccggg tccaccgctg ggtcggcggg
     6181 gccatggtcg gcggcgcctc cgtcaacgac ccggtgttct ggctgcacca cgccttcatc
     6241 gacctccagt ggtcccgctg gcaggcccgc caccgcggcg cccgctacct gcccgccgag
     6301 ccgcccggcc ggggcagcgc acagcgcgga cggatcgtcg cacggcacga gaagctgccg
     6361 ccgtgggacg tgacaccgga cgaactggag gacgtcggcc ggatctaccg ctacgcctga
     6421 gcgcgtccgt cccgggccgc gagcacatcg gtcccgggta cgggaggagc cccggccggg
     6481 aaggccgggg ctcctcggac gtcctgcgga ctcagccgcc gtagccgccg ctcttgtcgt
     6541 cgtcgtcgcc gttttcgcag gcgttgccga aggccgggtt cagcaggccg atcacgtcga
     6601 tggtgttgcc gcagaggttg accggcacgt gcaccgggac ctggacgacg ttgccggaca
     6661 ggacaccggg cgagccgacg gccgcgcccg ccgcgcccgc gtccgccatg gccatgccgg
     6721 tgccgctgag caccacggct ccggtgccca gggcgacacc cgcggccttc gcgatgcgag
     6781 acatcacgtt ctccttctga ctcggggagc gcggcggcag gacgcgccgc cgcactgccg
     6841 gttcaacgca cccggtcgca gcgggtcacg gggggaatgc gtggatcacc cgttttgaac
     6901 gcgaggtccg cgcgaggtcc gcgcgtggac cggaacggcg gcgtccggcg gaaaaccgtt
     6961 cccgtgcgca ccggtgtgtc cactagcctc ccggcatgcc gaacgaccga gccgactcgt
     7021 ccatcgatac ctccggggcc tccagggcct ccagggcctc cgaaacctcg cagtcttcgt
     7081 ccatgtcgtc catgtcgtcc acgtcgtcca tggcgtccgc gctggcggca ctgcccgctc
     7141 tgttcgccga ggagagccgg gtgcgggcct tcgcggccgt ggcgctgggc gcgggcgacg
     7201 ccgcgcaggt ggccggggcg gccggcctct ccgcgaagga ggcggccggc gcgctgcgcc
     7261 gactgcggga gcagggcgtg gtgacgtccg gggacgacgg cggactcgtc gtcgcccacg
     7321 gccgtttccg ggagctggcc cgcgtcggcc gtacggagtc ggcggcgccc cgggacgccg
     7381 ccgcggcggg cgacggcgga ccgggcgaca tgatcgtgcg gaccttcgtc aggaacggcc
     7441 ggctggtgcg tctccccgca cgctggacgc gcaagaaggt ggtgctgcgg cacatcgccg
     7501 agcagacctt cgcacccggc gtggagtacc cggagcgcac cgtcgacgag aagctgcgcg
     7561 cgtggtgcga ggacggcgag gtcgaccatg tgacgctccg gcgccacctg gtggacttcg
     7621 gtcacctgcg tcggagcgac ggcgtctacg cgcgcccggc ggactccgcc cggacgggca
     7681 cggcctgagt ccgcctacgc ccggctcggt gccggcggcg gggacccgac gggcagccgc
     7741 cgtatggggg agcccgcgag gtaggccccg atcgcctcga cggcctggcc gtagtacgtg
     7801 gcgtagttgg cgcgcgagac atagcccagg tggggtgtcg cgagcagccg cggggccgtg
     7861 cgcatcgggt ggccggcggg cagcggctcg gtgtcgaaga cgtccacgcc cgcacccgcg
     7921 atccggccct cgtgcagtgc ggtgagcagc gcctcctggt cgacgatcgc cgcgcgcgag
     7981 gtgttgacca ggtaggcggt cggcttgagc agggccagtt cggcggggcc gagcaggccg
     8041 cgggtgcgct cgcccagcgc caggtgcacg gagacgaagt cggcggtgcg caggagctct
     8101 tccttggagg aggccagttc cacaccgacc tcgtcggtgc gttcccgggt caggttctgg
     8161 ctccaggcgc tcacccgcat gccgaaggcc aggccgacct gggccacccg cccgccgatc
     8221 ttgcccaggc ccagcagtcc gagccggcgg ccgtgcaggt cggcgcccac cgtctgctgc
     8281 caggggccgc cctcgcgcag cccgttggcc tcctggacga tgccgcgggc gaggccgagg
     8341 atcagggccc aggtcagttc gacgggcggg gtcgaggagg aggccgtacc gcagacggtg
     8401 acgccgtgcg cgtcggcggc ggcgtagtcg atcacgctgt tgcgcatgcc ggaggcgacc
     8461 agcagcttca gccgcggcag ccgggcgatc aacgagccgg ggaacggcac gcgttcgcgc
     8521 agcgtgacca cgatgtcggc gtccgcgagc gccgcggcga gggcgtcctc gtccgggaag
     8581 tgggtgccgt gggacacgac ctccacccgg tcggcgaccg gcgaccagtc ggccgcctcg
     8641 gtcgccaccc cctgatagtc gtcgagtacg gcgcagcgca gtcgcacggt gggtggtccc
     8701 tctcgccgaa ggggcagtgg cggccgccga gtctaccgag gcctcacgcg gaggcggagg
     8761 cggaggccga ggaggtcccg gcggccggcg ccgccagccg cagcccgacg ccgatcgccg
     8821 ccagatcctc ctcgtacgcg ctgtggcggc gggcgcacct ggccaggtcg cggtcgtgcc
     8881 ggaaggcgcc accgcgggtg atgtgggggt cgaaggccca gtcctcgacc aggggtacgc
     8941 cggcgggggc gccggggtac ggggcgtaca gcgtggaggt ccactcgtcc acgttgcccg
     9001 ccatgtccag caccccgaac gggccggcgc cgtccgggaa cgagccgacc ggggtggtcg
     9061 tgccgacacc gaggtccacc aggttggcca ggccggtgcg gtactcgtcg ccccacggga
     9121 actcccggcc gtcgtcgccg cgggccgcgc gctcccattc gtcctccgtg gggagcctga
     9181 tgcccggatc gccgctcagc tcgccgagcc accggcagta ggccgacgcc gcctcccagc
     9241 tcacgccggt gaccggatgg tcgtgcggag ccgttggcac cggccggccg gtggccgcgg
     9301 cgaagacgga ccactggccg accgtgaccg gggtacgggc gagccggaag gccggaaggc
     9361 ggatctccgt gctcggcgcc tccttgaggt accactcgac cggcaccccg gtgtcggcgt
     9421 aacgctcggc caccagcgga atgtcgtccg ccggggtgcc ccggcgcacc acccccgccg
     9481 ggatctccac ccagtccacc gcttcgatcc gcatcgctcc tcctcggccc gtccaaccgt
     9541 ccaaccgtcc caccgtccag tgcgaccacc atgatcgcgc ctgtccgccg ggggcgtacc
     9601 cggccgtgga cggagtccga ccgggactta ggcactagga ccccgccccc gcgtgggcga
     9661 ggagcgccgc cccgtaggcc cgcaggaccc ggtgctcccg cgcgtggacg acggggacgc
     9721 ggccgcccgg acccgccggg tcgcattcgg tcaccaccgc ggcatggcgc agcagcacat
     9781 agccgtcgga cgccgcggcg tggaaggccg acggatctcc gcgcgccaac tcggcccact
     9841 cctggtgccc gtcgcccagc gccgcgcggg acgcctccag caggcccggc tcgcacagac
     9901 ccggccgcag ggtgtccacg gcgatgccgt ccaccgtggt ggcgaggtcg acgcagaccg
     9961 ggccgtcgcc caaccgctgt tccccgagcg cgcgcagcgc ggtctcggcg gcccagtcct
    10021 gctccgtgcc acgacggggg agcaggagcg cgaagacggg ctcgcaggcc ggccgtgggc
    10081 cggccggcct gctcactcgc cagatcgcgc agatccggca ggagtccccg agagctacgg
    10141 cgttgatgtg cagccccgtg atcaagggtg gtgacatgcc ctcggctccc ggtcggcggc
    10201 ggtgctcagt gactcagcac gtgaccgtcg ccggggcgat ggtcacgcga gcgtcgcagg
    10261 tcggccaccg cgaaggtgcg cagcagccag cggtcgcccc cgtcgtagcg tggccggaag
    10321 gccgtcctgc cgtgcgtggt cacccggttg tccacgatgg cgaggtctcc cggcgtcaga
    10381 cgcaccgtgt gggacgcggc gtcgacggcc gcctccaact cgaccagcgc ccggcgcgcc
    10441 tcgggcgtca cggccgtggt ggcggagaag tccacccgca ggtccgggtc ctccgggtcg
    10501 ccggtcagca ccgggcgtgg cggcgtggtc ccggcggccg tcccgaagga gggcggcggc
    10561 tgggtgctga
//
