GCF_014650695.1 - 50 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_BMVL01000001.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	NI-siderophore	844,501	874,282	High	desferrioxamin B/desferrioxamine E	other:other

NZ_BMVL01000002.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 2.1	NRPS,NRPS-like	1	38,404	High	antipain	NRPS:Type I
Region 2.2	NAPAA	129,885	163,796	High	ε-Poly-L-lysine	NRPS:Type I
Region 2.3	CDPS	164,051	184,791
Region 2.4	T3PKS	228,688	269,749	High	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone/2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	PKS:Type III
Region 2.5	NI-siderophore,lanthipeptide-class-i	292,283	334,473
Region 2.6	terpene	545,324	573,751	High	isorenieratene	terpene
Region 2.7	melanin	681,030	708,356
Region 2.8	terpene	712,675	733,580
Region 2.9	terpene	793,121	814,494	High	2-methylisoborneol	terpene

NZ_BMVL01000003.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	T1PKS,NRPS	18,932	83,130	Medium	coelichelin	NRPS:Type I
Region 3.2	NRPS-like,NRPS	144,762	214,597	High	JBIR-126	NRPS:Type I
Region 3.3	T2PKS	497,661	570,155	Medium	spore pigment	PKS
Region 3.4	ectoine	630,150	640,554	High	ectoine	other:other

NZ_BMVL01000004.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 4.1	hydrogen-cyanide	1	12,221	Low	aborycin	ribosomal:RiPP
Region 4.2	RiPP-like	71,563	82,900	Low	14-hydroxyisochainin	PKS:Modular type I
Region 4.3	terpene	199,201	221,417	High	geosmin	terpene:Sesquiterpene
Region 4.4	T1PKS	269,503	315,868
Region 4.5	terpene	557,888	584,881	Medium	hopene	terpene

NZ_BMVL01000005.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 5.1	T1PKS,hglE-KS	13,909	65,573
Region 5.2	NRPS	98,041	185,101	High	leupeptin Pr/leupeptin Ac	NRPS:Type I
Region 5.3	terpene	433,327	454,424
Region 5.4	lanthipeptide-class-iii,terpene-precursor	539,615	571,517	High	SapB	ribosomal:RiPP:Lanthipeptide

NZ_BMVL01000006.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	NRPS-like,terpene	3,187	49,760	Low	lipstatin	NRPS:Type I
Region 6.2	NRP-metallophore,NRPS	342,946	409,546	Low	peucechelin	NRPS:Type I

NZ_BMVL01000008.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 8.1	terpene	261,638	282,267	Low	GP6738	NRPS:Type I

NZ_BMVL01000010.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	terpene-precursor	221,041	242,084

NZ_BMVL01000013.1 (Streptomyces avidinii)

Region	Type	From	To
Region 13.1	NRPS	1	24,783
Region 13.2	lanthipeptide-class-ii	45,626	71,870
Region 13.3	NI-siderophore	145,617	178,247

NZ_BMVL01000014.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 14.1	T1PKS	1	24,294	Low	oligomycin	PKS

NZ_BMVL01000018.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 18.1	butyrolactone	1	8,064
Region 18.2	NRPS	41,326	86,062

NZ_BMVL01000021.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 21.1	NRPS	82,340	107,518	Low	enduracidin A/enduracidin B	NRPS:Type I

NZ_BMVL01000022.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 22.1	T1PKS	1	71,477	Medium	oligomycin	PKS

NZ_BMVL01000023.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 23.1	azole-containing-RiPP	39,624	69,385	Medium	lactazole	ribosomal:RiPP:Thiopeptide

NZ_BMVL01000028.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 28.1	butyrolactone	18,005	26,839

NZ_BMVL01000031.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 31.1	NRPS	1	19,625

NZ_BMVL01000032.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 32.1	T1PKS	1	18,572	Low	oligomycin	PKS

NZ_BMVL01000033.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 33.1	ectoine	7,835	15,455

NZ_BMVL01000034.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 34.1	NRPS	1	13,949

NZ_BMVL01000035.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 35.1	NRPS	1	13,167

NZ_BMVL01000037.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 37.1	NRPS	1	12,079

NZ_BMVL01000039.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 39.1	NRPS	1	5,948

NZ_BMVL01000040.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 40.1	T1PKS	1	5,742

NZ_BMVL01000045.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 45.1	NRPS	1	3,226

NZ_BMVL01000049.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 49.1	NRPS	1	2,036

NZ_BMVL01000050.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 50.1	NRPS	1	1,924	High	rhizomide A/rhizomide B/rhizomide C	NRPS:Type I

NZ_BMVL01000051.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 51.1	NRPS	1	1,909	High	rhizomide A/rhizomide B/rhizomide C	NRPS:Type I

NZ_BMVL01000052.1 (Streptomyces avidinii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 52.1	NRPS	1	1,894

No secondary metabolite regions were found in these records:: NZ_BMVL01000007.1 (Streptomyces avidinii); NZ_BMVL01000009.1 (Streptomyces avidinii); NZ_BMVL01000011.1 (Streptomyces avidinii); NZ_BMVL01000012.1 (Streptomyces avidinii); NZ_BMVL01000015.1 (Streptomyces avidinii); NZ_BMVL01000016.1 (Streptomyces avidinii); NZ_BMVL01000017.1 (Streptomyces avidinii); NZ_BMVL01000019.1 (Streptomyces avidinii); NZ_BMVL01000020.1 (Streptomyces avidinii); NZ_BMVL01000024.1 (Streptomyces avidinii); NZ_BMVL01000025.1 (Streptomyces avidinii); NZ_BMVL01000026.1 (Streptomyces avidinii); NZ_BMVL01000027.1 (Streptomyces avidinii); NZ_BMVL01000029.1 (Streptomyces avidinii); NZ_BMVL01000030.1 (Streptomyces avidinii); NZ_BMVL01000036.1 (Streptomyces avidinii); NZ_BMVL01000038.1 (Streptomyces avidinii); NZ_BMVL01000041.1 (Streptomyces avidinii); NZ_BMVL01000042.1 (Streptomyces avidinii); NZ_BMVL01000043.1 (Streptomyces avidinii); NZ_BMVL01000044.1 (Streptomyces avidinii); NZ_BMVL01000046.1 (Streptomyces avidinii); NZ_BMVL01000047.1 (Streptomyces avidinii); NZ_BMVL01000048.1 (Streptomyces avidinii); NZ_BMVL01000053.1 (Streptomyces avidinii); NZ_BMVL01000054.1 (Streptomyces avidinii); NZ_BMVL01000055.1 (Streptomyces avidinii); NZ_BMVL01000056.1 (Streptomyces avidinii); NZ_BMVL01000057.1 (Streptomyces avidinii); NZ_BMVL01000058.1 (Streptomyces avidinii)

Compact view

NZ_BMVL01000001.1 - Region 1 - NI-siderophore

Download region GenBank file

Download region SVG

Location: 844,501 - 874,282 nt. (total: 29,782 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

IE257_RS03895: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

IE257_RS03915: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

Transferases: carboxylase transferase

IE257_RS03880: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

IE257_RS03885: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Lyases: decarboxylase

IE257_RS03910: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: synthase

IE257_RS03890: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000941.3	0.83	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001478.5	0.83	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002305.2	0.82	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000940.5	0.75	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.75	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0001572.3	0.70	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002464.3	0.61	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0002284.3	0.60	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002690.2	0.60	other (other)	FW0622	Verrucosispora sp. FIM060022
BGC0002620.2	0.55	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000941.3	0.83	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001478.5	0.83	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002305.2	0.82	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000940.5	0.75	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.75	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0001572.3	0.70	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002464.3	0.61	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0002284.3	0.60	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002690.2	0.60	other (other)	FW0622	Verrucosispora sp. FIM060022
BGC0002620.2	0.55	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.77 out of 36.53

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 31.36 out of 36.53

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 25.84 out of 30.12

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 22.70 out of 27.04

Confidence: medium

Additional weak hits (5):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.48 out of 27.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.21 out of 29.65

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.98 out of 25.88

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.26 out of 33.14

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
DasR	N-acetylglucosamine dependent repressor
DmdR1	Iron(II)-dependent repressor
OsdR	Development and stress management regulator

NZ_BMVL01000002.1 - Region 1 - NRPS,NRPS-like

Download region GenBank file

Download region SVG

Location: 1 - 38,404 nt. (total: 38,404 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

IE257_RS04135: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acyltransferase

IE257_RS04165: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Ligases: ligase

IE257_RS04170: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS04175: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS04180: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS04185: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS04195: carboxylate-amine ligase

smcogs: SMCOG1165: carboxylate-amine ligase

IE257_RS04210: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS04225: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002051.2	1.07	NRPS	antipain	Streptomyces sp.
BGC0001249.5	0.84	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.75	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000342.4	0.71	NRPS	enniatin	Fusarium equiseti
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001570.5	0.69	NRPS	deimino-antipain	Streptomyces albulus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002051.2	0.80	NRPS	antipain	Streptomyces sp.
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001570.5	0.68	NRPS	deimino-antipain	Streptomyces albulus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 25.16 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 26.03 out of 30.11

Confidence: medium

Additional weak hits (2):

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.85 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 38404:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Arg) + (Phe) + (Val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 33295:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Arg) + (Phe) + (Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS04170: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (24..412): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LAPSFDASTFEGFFVVGGEVNGYGPTETTTFATA
Nearest Stachelhaus code(s):: Val DAFFVGGTFK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS04175: Phe

Search NORINE for peptide: strict or relaxed

AMP-binding (41..446): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AWYIFDPHLLESVAVVGGDHNAYGPAEATVVTTT
Nearest Stachelhaus code(s):: Phe DPLVVGAVVK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS04180: Arg

Search NORINE for peptide: strict or relaxed

AMP-binding (462..852): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HATAFDVALEEPNLVLGGELNVYGVTEAAISSTV
Nearest Stachelhaus code(s):: Arg DVENVGVISK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS04210: X

AMP-binding (42..456): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NFVVFGMVMGNAYGIMAGSSICYGMTETSPVSTQ
Nearest Stachelhaus code(s):: Abu or Ival DMGFIAGVVK (38% 8Å match)
Stachelhaus code match:: 60% (weak)

IE257_RS04225: Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (14..363): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DALH---LVLGGFGVSGSAVERYGMTETLITLSG
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CelR	Cellobiose uptake repressor

NZ_BMVL01000002.1 - Region 2 - NAPAA

Download region GenBank file

Download region SVG

Location: 129,885 - 163,796 nt. (total: 33,912 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS04760: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: hydrolase

IE257_RS04820: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

Ligases: ligase

IE257_RS04770: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0002135.2	0.60	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.59	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.59	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0002135.2	0.60	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.59	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.59	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.19 out of 19.26

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.42 out of 20.47

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 24.03 out of 25.31

Confidence: strong

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

Additional weak hits (14):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.41 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.54 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.98 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.23 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.80 out of 27.78

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.44 out of 36.54

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.27 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.09 out of 29.38

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.33 out of 30.25

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.31 out of 21.55

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.57 out of 32.93

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.25 out of 26.85

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.07 out of 33.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.54 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 129884 - 163796:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS04770: X

AMP-binding (42..419): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAELFDASCEELSTIFGGEWNTYGPTEATVVACA
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (71% 8Å match); Gln or Tyr DAESVGTVVK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000002.1 - Region 3 - CDPS

Download region GenBank file

Download region SVG

Location: 164,051 - 184,791 nt. (total: 20,741 nt) Show pHMM detection rules used

CDPS: CDPS

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

IE257_RS04890: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Hydrolases: hydrolase

IE257_RS04880: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

IE257_RS04895: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001948.3	0.63	other (other)	naseseazine C, C3-aryl pyrroloindolines	Streptomyces sp.
BGC0001986.3	0.59	other (other)	purincyclamide	Streptomyces chrestomyceticus
BGC0000851.5	0.46	other (other)	albonoursin	Streptomyces noursei ATCC 11455
BGC0000902.4	0.46	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000285.5	0.41	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002000.4	0.40	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0002007.2	0.40	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0002582.3	0.39	NRPS	guanipiperazine A, guanipiperazine B	Streptomyces chrestomyceticus
BGC0002514.3	0.38	PKS	julichrome Q6.6	Streptomyces sampsonii
BGC0002482.2	0.37	other (other)	tjipanazole D, tjipanazole I, tjipanazole L, tjipanazole K, tjipanazole M	Symphyonema bifilamentata 97.28

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001948.3	0.63	other (other)	naseseazine C, C3-aryl pyrroloindolines	Streptomyces sp.
BGC0001986.3	0.59	other (other)	purincyclamide	Streptomyces chrestomyceticus
BGC0000851.5	0.46	other (other)	albonoursin	Streptomyces noursei ATCC 11455
BGC0000902.4	0.46	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000285.5	0.41	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002000.4	0.40	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0002007.2	0.40	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0002582.3	0.39	NRPS	guanipiperazine A, guanipiperazine B	Streptomyces chrestomyceticus
BGC0002514.3	0.38	PKS	julichrome Q6.6	Streptomyces sampsonii
BGC0002482.2	0.37	other (other)	tjipanazole D, tjipanazole I, tjipanazole L, tjipanazole K, tjipanazole M	Symphyonema bifilamentata 97.28

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (11):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.60 out of 26.12

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.76 out of 30.25

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.79 out of 20.01

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.33 out of 30.97

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.14 out of 33.14

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.47 out of 27.04

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.50 out of 29.51

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.86 out of 27.55

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.06 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.06 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.38 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description

NZ_BMVL01000002.1 - Region 4 - T3PKS

Download region GenBank file

Download region SVG

Location: 228,688 - 269,749 nt. (total: 41,062 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

IE257_RS05250: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

IE257_RS05285: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

Transferases: acyltransferase methyltransferase

IE257_RS05175: methyltransferase

smcogs: SMCOG1089: methyltransferase

IE257_RS05220: acyltransferase

smcogs: SMCOG1141: acyltransferase

IE257_RS05280: Methylation

MITE: MITE0000155Methylation (identity: 70.70%)
smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

Hydrolases: hydrolase

IE257_RS05185: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase synthase

IE257_RS05180: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS05275: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.74	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001831.3	0.53	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0001962.3	0.48	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0001077.4	0.46	PKS	6-anteisoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-geranyl-2-methoxyhydroquinone, 6-palmityl-4-O-geranyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-palmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone	Actinoplanes missouriensis 431
BGC0002362.3	0.44	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0000343.5	0.43	NRPS	enterobactin	Pseudomonas sp. J465
BGC0002332.2	0.43	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0002347.3	0.42	other (other)	cyanogramide, cyanogramide B, cyanogramide C, cyanogramide D	Actinoalloteichus caeruleus WH1-2216-6
BGC0002474.2	0.42	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0001844.4	0.42	NRPS	holrhizin A, holrhizin B, holrhizin C, holrhizin D	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.74	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001831.3	0.53	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0001962.3	0.48	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0001077.4	0.46	PKS	6-anteisoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-geranyl-2-methoxyhydroquinone, 6-palmityl-4-O-geranyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-palmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone	Actinoplanes missouriensis 431
BGC0002362.3	0.44	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0000343.5	0.43	NRPS	enterobactin	Pseudomonas sp. J465
BGC0002332.2	0.43	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0002347.3	0.42	other (other)	cyanogramide, cyanogramide B, cyanogramide C, cyanogramide D	Actinoalloteichus caeruleus WH1-2216-6
BGC0002474.2	0.42	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0001844.4	0.42	NRPS	holrhizin A, holrhizin B, holrhizin C, holrhizin D	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (9):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.69 out of 29.38

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.53 out of 27.55

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.68 out of 26.85

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.84 out of 28.14

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.08 out of 30.22

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.68 out of 30.97

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.71 out of 29.65

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 20.79 out of 36.53

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.80 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

IE257_RS05180: X

AMP-binding (31..412): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: PWAR-GGRCLERYLSYGDSRQGYATNEAAVICRL
Nearest Stachelhaus code(s):: Ile DALHSGAVIK (29% 8Å match); OH-Orn DGEGSGGVTK (21% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_BMVL01000002.1 - Region 5 - NI-siderophore,lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 292,283 - 334,473 nt. (total: 42,191 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC
lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase

IE257_RS05560: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Transferases: methyltransferase

IE257_RS05555: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: hydrolase

IE257_RS05575: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: cyclase dehydratase

IE257_RS05615: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

IE257_RS05620: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Translocases: transport

IE257_RS05510: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.45	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001289.5	0.33	ribosomal	penisin	Paenibacillus ehimensis
BGC0000541.5	0.30	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000542.3	0.30	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000515.4	0.29	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000560.5	0.28	ribosomal	subtilomycin	Bacillus subtilis
BGC0000544.4	0.28	ribosomal	planosporicin	Planomonospora alba
BGC0001727.3	0.27	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000540.5	0.26	ribosomal	paenibacillin	Paenibacillus polymyxa OSY-DF
BGC0002668.2	0.24	ribosomal	cesin A	Clostridium estertheticum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000528.5	0.54	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.52	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000503.5	0.51	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000515.4	0.51	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000544.4	0.51	ribosomal	planosporicin	Planomonospora alba
BGC0002346.3	0.50	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0001289.5	0.50	ribosomal	penisin	Paenibacillus ehimensis
BGC0000541.5	0.49	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000542.3	0.49	ribosomal	paenicidin B	Paenibacillus terrae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 22.63 out of 27.04

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.76 out of 25.31

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (8):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.99 out of 29.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.37 out of 31.73

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.40 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.59 out of 29.38

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.42 out of 26.12

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.32 out of 30.98

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.13 out of 35.63

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 161 - 252; Score: 40.1

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor
DasR	N-acetylglucosamine dependent repressor

NZ_BMVL01000002.1 - Region 6 - terpene

Download region GenBank file

Download region SVG

Location: 545,324 - 573,751 nt. (total: 28,428 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS06880: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: methyltransferase

IE257_RS06860: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: synthetase

IE257_RS06885: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.82	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.75	terpene	isorenieratene	Streptomyces argillaceus
BGC0000649.3	0.72	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.71	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.70	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000648.3	0.53	terpene	carotenoid	Myxococcus xanthus
BGC0000640.3	0.50	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000639.3	0.49	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000641.3	0.48	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0002309.2	0.47	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.82	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.75	terpene	isorenieratene	Streptomyces argillaceus
BGC0000649.3	0.72	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.71	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.70	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000648.3	0.53	terpene	carotenoid	Myxococcus xanthus
BGC0000640.3	0.50	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000639.3	0.49	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000641.3	0.48	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0002309.2	0.47	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

IE257_RS06855: Lycopene_cycl

Domain 1 (80 - 459): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

IE257_RS06875: PT_phytoene_like

Domain 1 (17 - 307): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → diapophytoene; FPP → squalene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

IE257_RS06885: PT_FPPS_like

Domain 1 (64 - 385): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.50 out of 20.47

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 24.84 out of 29.72

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.79 out of 25.31

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (5):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.31 out of 39.62

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.63 out of 27.78

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.36 out of 35.63

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.94 out of 19.52

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
NtrC	Nitrogen regulatory protein C
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000002.1 - Region 7 - melanin

Download region GenBank file

Download region SVG

Location: 681,030 - 708,356 nt. (total: 27,327 nt) Show pHMM detection rules used

melanin: melC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS07525: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

IE257_RS07530: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

IE257_RS07535: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acetyltransferase methyltransferase

IE257_RS07425: methyltransferase

smcogs: SMCOG1089: methyltransferase

IE257_RS07480: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: esterase hydrolase protease

IE257_RS07460: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

IE257_RS07475: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

IE257_RS07490: putative esterase

smcogs: SMCOG1104: putative esterase

Lyases: decarboxylase

IE257_RS07430: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000910.3	0.81	other (other)	melanin	Streptomyces coelicolor A3(2)
BGC0000911.3	0.80	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000909.3	0.64	other (other)	melanin	Streptomyces avermitilis
BGC0000908.3	0.63	other (other)	melanin	Streptomyces avermitilis
BGC0000912.3	0.61	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002284.3	0.45	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000255.5	0.45	PKS	pederin	Uncultured bacterium
BGC0000885.3	0.44	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000889.5	0.44	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344
BGC0000662.5	0.43	terpene	grixazone A	Streptomyces griseus subsp. griseus NBRC 13350

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000910.3	0.81	other (other)	melanin	Streptomyces coelicolor A3(2)
BGC0000911.3	0.80	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000909.3	0.64	other (other)	melanin	Streptomyces avermitilis
BGC0000908.3	0.63	other (other)	melanin	Streptomyces avermitilis
BGC0000912.3	0.61	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002284.3	0.45	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000255.5	0.45	PKS	pederin	Uncultured bacterium
BGC0000885.3	0.44	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000889.5	0.44	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344
BGC0000662.5	0.43	terpene	grixazone A	Streptomyces griseus subsp. griseus NBRC 13350

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

Additional weak hits (10):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.24 out of 26.85

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.83 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.82 out of 28.14

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.62 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.57 out of 29.38

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 17.67 out of 22.40

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.29 out of 18.57

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.21 out of 20.01

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.71 out of 24.78

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_BMVL01000002.1 - Region 8 - terpene

Download region GenBank file

Download region SVG

Location: 712,675 - 733,580 nt. (total: 20,906 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS07590: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001810.3	0.41	PKS	trehangelin	Polymorphospora rubra
BGC0000189.4	0.40	PKS	3-(2'-hydroxy-3'-oxo-4'-methylpentyl)-indole, 3-(2'-hydroxy-3'-oxo-4'-methylhexyl)-indole, 3-(2'-acetoxy-3'-oxo-4'-methylpentyl)-indole, 3-(2'-acetoxy-3'-oxo-4'-methylhexyl)-indole	Xenorhabdus bovienii SS-2004
BGC0000843.5	0.39	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064
BGC0001521.3	0.39	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0002584.2	0.37	ribosomal	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0000391.5	0.35	NRPS	methylpendolmycin, pendolmycin	Marinactinospora thermotolerans
BGC0002294.3	0.35	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0002436.2	0.34	other (other)	coformycin	Streptomyces citricolor
BGC0000194.5	0.34	PKS	actinorhodin	Streptomyces coelicolor A3(2)
BGC0000713.5	0.33	saccharide	ribostamycin	Streptomyces ribosidificus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001810.3	0.41	PKS	trehangelin	Polymorphospora rubra
BGC0000189.4	0.40	PKS	3-(2'-hydroxy-3'-oxo-4'-methylpentyl)-indole, 3-(2'-hydroxy-3'-oxo-4'-methylhexyl)-indole, 3-(2'-acetoxy-3'-oxo-4'-methylpentyl)-indole, 3-(2'-acetoxy-3'-oxo-4'-methylhexyl)-indole	Xenorhabdus bovienii SS-2004
BGC0000843.5	0.39	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064
BGC0001521.3	0.39	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0002584.2	0.37	ribosomal	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0000391.5	0.35	NRPS	methylpendolmycin, pendolmycin	Marinactinospora thermotolerans
BGC0002294.3	0.35	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0002436.2	0.34	other (other)	coformycin	Streptomyces citricolor
BGC0000194.5	0.34	PKS	actinorhodin	Streptomyces coelicolor A3(2)
BGC0000713.5	0.33	saccharide	ribostamycin	Streptomyces ribosidificus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none
triterpene		squalene	C30	acyclic	none
triterpene		PSPP	C30	acyclic	PP
tetraterpene	C40 carotenoid	phytoene	C40	acyclic	none
triterpene		hydroxysqualene	C30	acyclic	OH

Genes with terpene domains:

IE257_RS07610: PT_phytoene_like

Domain 1 (60 - 283): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → diapophytoene; FPP → squalene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

Additional weak hits (8):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.41 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.99 out of 27.78

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.87 out of 27.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.57 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.38 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.17 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.63 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.02 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_BMVL01000002.1 - Region 9 - terpene

Download region GenBank file

Download region SVG

Location: 793,121 - 814,494 nt. (total: 21,374 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS08000: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IE257_RS08055: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IE257_RS08060: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: methyltransferase

IE257_RS08020: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase

IE257_RS08025: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000658.3	0.86	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000659.3	0.79	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000657.5	0.64	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000513.3	0.42	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0002496.3	0.41	NRPS	serratiochelin A, serratiochelin C, serratiochelin B	Serratia plymuthica
BGC0000188.5	0.40	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000631.5	0.39	terpene	Botrydial, 8,9-epibotrydial, 1-epibotrydial, Dihydrobotrydial, 10-Oxo-dihydrobotrydial, 10-dehydroxy dihydrobotrydialone, 10-epi-dihydrobotrydial, Botryendial, Botryenalol, Botrydienal, Norbotrydialone acetate, 10-Oxodihydrobotry-1(9),4(5)-diendial, 10-Oxodehydrodihydrobotrydial, Dehydrobotrydienol, 11-Hydroxydehydrobotrydienol, 12-hydroxydehydrobotrydienol, Secobotrytriendiol, Secobotrytriene-10,12,15-triol, Secobotrydiene-3,10,15-triol, Secobotrydiene-3,4,10,15-tetraol, 4β-Acetoxytetrahydrobotryslactone	Botrytis cinerea B05.10
BGC0000234.5	0.38	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000509.4	0.38	ribosomal	epilancin 15x	Staphylococcus epidermidis
BGC0002635.2	0.38	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000658.3	0.86	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000659.3	0.79	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000657.5	0.64	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000513.3	0.42	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0002496.3	0.41	NRPS	serratiochelin A, serratiochelin C, serratiochelin B	Serratia plymuthica
BGC0000188.5	0.40	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000631.5	0.39	terpene	Botrydial, 8,9-epibotrydial, 1-epibotrydial, Dihydrobotrydial, 10-Oxo-dihydrobotrydial, 10-dehydroxy dihydrobotrydialone, 10-epi-dihydrobotrydial, Botryendial, Botryenalol, Botrydienal, Norbotrydialone acetate, 10-Oxodihydrobotry-1(9),4(5)-diendial, 10-Oxodehydrodihydrobotrydial, Dehydrobotrydienol, 11-Hydroxydehydrobotrydienol, 12-hydroxydehydrobotrydienol, Secobotrytriendiol, Secobotrytriene-10,12,15-triol, Secobotrydiene-3,10,15-triol, Secobotrydiene-3,4,10,15-tetraol, 4β-Acetoxytetrahydrobotryslactone	Botrytis cinerea B05.10
BGC0000234.5	0.38	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000509.4	0.38	ribosomal	epilancin 15x	Staphylococcus epidermidis
BGC0002635.2	0.38	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
monoterpene		2-methylisoborneol	C11	C1-C6	OH
monoterpene		2-methylenebornane	C11	C1-C6	none

Genes with terpene domains:

IE257_RS08025: T1TS

Domain 1 (61 - 451): Type 1 terpene synthase

Subtype: 2-Methylisoborneol synthase type

Reactions:: 2-MeGPP → 2-methylisoborneol; 2-MeGPP → 2-methylenebornane

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.42 out of 19.52

Confidence: strong

Additional weak hits (5):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.89 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.36 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.13 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.68 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.29 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
2-MeGPP	2-methylgeranyl diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production

NZ_BMVL01000003.1 - Region 1 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 18,932 - 83,130 nt. (total: 64,199 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS08430: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: acylase

IE257_RS08360: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

IE257_RS08415: enterobactin/ferric enterobactin esterase

smcogs: SMCOG1098: enterobactin/ferric enterobactin esterase

Ligases: ligase

IE257_RS08405: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Translocases: transport

IE257_RS08365: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000357.5	0.69	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0000325.5	0.67	NRPS	coelichelin	Streptomyces coelicolor A3(2)
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001240.4	0.61	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001276.3	0.59	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.58	PKS	asperlactone	Aspergillus ochraceus
BGC0001261.3	0.58	NRPS	AM-toxin	Alternaria alternata
BGC0001244.3	0.58	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.57	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001641.4	0.57	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000325.5	0.70	NRPS	coelichelin	Streptomyces coelicolor A3(2)
BGC0000357.5	0.67	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001240.4	0.64	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001261.3	0.63	NRPS	AM-toxin	Alternaria alternata
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.62	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.75 out of 23.43

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 28.73 out of 36.53

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.71 out of 20.47

Confidence: medium

Additional weak hits (8):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.56 out of 21.55

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.85 out of 36.53

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.88 out of 39.62

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.23 out of 31.73

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 26.30 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.30 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.71 out of 36.53

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.52 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 18931 - 83130:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (D-Fo-OH-Orn - D-Thr - OH-Orn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 18931 - 63662:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (D-Fo-OH-Orn - D-Thr - OH-Orn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 32183 - 83130:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (D-Fo-OH-Orn - D-Thr - OH-Orn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS08360: mal

PKS_AT (580..907): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 83.3
Methylmalonyl-CoA: 48.3
Methoxymalonyl-CoA: 40.7
Propionyl-CoA: 36.1
inactive: 29.3
fatty_acid: 25.4
Ethylmalonyl-CoA: 25.4
2-Methylbutyryl-CoA: 18.9
CHC-CoA: 13.3
trans-1,2-CPDA: 13.2
Isobutyryl-CoA: 10.7
Benzoyl-CoA: 8.5
Acetyl-CoA: 8.0
3-Methylbutyryl-CoA: 5.6

IE257_RS08405: Fo-OH-Orn - Thr - OH-Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (147..545): Fo-OH-Orn

nrpys: Fo-OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: RWQAFDISINELYLWCGGEYHGYGPAETTIGVSH
Nearest Stachelhaus code(s):: Fo-OH-Orn DINYWGGIGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1632..2047): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LNTHFDFAVWEGNQIFGGEVNMYGITETTVHVTH
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3067..3467): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: MNHVFDMSWEEWNVLLGGEYNLYGPTEYTINTLG
Nearest Stachelhaus code(s):: OH-Orn DMENLGLINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
BldD	Development and antibiotic global regulator
DmdR1	Iron(II)-dependent repressor
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000003.1 - Region 2 - NRPS,NRPS-like

Download region GenBank file

Download region SVG

Location: 144,762 - 214,597 nt. (total: 69,836 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

IE257_RS08790: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

IE257_RS08840: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

IE257_RS08855: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

IE257_RS08875: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

IE257_RS08915: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

IE257_RS08920: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

IE257_RS09015: NADH:flavin oxidoreductase/NADH oxidase

smcogs: SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase

Transferases: PPTase halogenase

IE257_RS08845: flavin-dependent trp 6 7 halogenase

halogenases: flavin-dependent trp 6 7 halogenase
MITE: MITE0000130Halogenation (identity: 71.90%)
smcogs: SMCOG1119: halogenase

IE257_RS08885: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: esterase hydrolase

IE257_RS08805: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

IE257_RS08865: thioesterase

smcogs: SMCOG1004: thioesterase

Isomerases: mutase

IE257_RS08820: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase

IE257_RS08860: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS08880: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS08900: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS08905: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS08910: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS08945: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Translocases: permease

IE257_RS08825: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001368.5	1.03	NRPS	JBIR-126	Streptomyces sp. NRRL F-4474
BGC0002075.3	0.88	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.83	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.79	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.78	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.77	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.75	NRPS	AM-toxin	Alternaria alternata
BGC0001825.4	0.74	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000342.4	0.73	NRPS	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001368.5	0.86	NRPS	JBIR-126	Streptomyces sp. NRRL F-4474
BGC0002075.3	0.75	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.00 out of 20.47

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.71 out of 20.47

Confidence: medium

Additional weak hits (14):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.62 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.94 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.26 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.77 out of 26.06

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.36 out of 30.97

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.28 out of 27.04

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 12.94 out of 18.57

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.76 out of 18.57

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.93 out of 31.73

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.62 out of 25.75

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.73 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.98 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.77 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.61 out of 20.01

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 144761 - 214597:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (Lys) + (X) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 144761 - 186666:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (Lys) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 6, location 148963 - 214597:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (Lys) + (X) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS08860: X

AMP-binding (47..433): X

nrpys: meSer

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FALVFDGWLWCLSLVVAGELNVYGPTEVTIAATW
Nearest Stachelhaus code(s):: meSer DGWSVAVIAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS08880: X

AMP-binding (24..426): X

nrpys: 6-clTrp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWTSFAVQIMEAMLVTTAEVMTYGTTETTNVLSC
Nearest Stachelhaus code(s):: 6-clTrp AVMMVTTNVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS08900: Lys

Search NORINE for peptide: strict or relaxed

AMP-binding (506..914): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: NDLSFDMSVYEGNSFLGGDVALGGATEASIHSVE
Nearest Stachelhaus code(s):: Lys DMYNFGLIHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS08905: X

AMP-binding (509..898): X

nrpys: meSer

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FTIAFDVSVFETELSVGGEVNGYGPTEATVAVTL
Nearest Stachelhaus code(s):: meSer DVFESGGVAK (100% 8Å match); Lys DVFESGGVAK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS08945: X

AMP-binding (476..881): X

nrpys: me-clHic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: LDVSFDASIYDGNSVLSGDVGSGGPTETICWSLF
Nearest Stachelhaus code(s):: me-clHic DAYNVSSCWK (100% 8Å match); meSer DAYNVSSCWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000003.1 - Region 3 - T2PKS

Download region GenBank file

Download region SVG

Location: 497,661 - 570,155 nt. (total: 72,495 nt) Show pHMM detection rules used

T2PKS: (t2ks and t2clf)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

IE257_RS10480: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

IE257_RS10555: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

IE257_RS10605: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acetyltransferase

IE257_RS10375: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: protease

IE257_RS10385: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase

IE257_RS10510: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

IE257_RS10515: Polyketide synthesis cyclase

smcogs: SMCOG1225: Polyketide synthesis cyclase

Ligases: synthase

IE257_RS10495: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

IE257_RS10500: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000272.3	0.74	PKS	spore pigment	Streptomyces collinus
BGC0000271.5	0.71	PKS	spore pigment	Streptomyces avermitilis
BGC0000215.5	0.69	PKS	curamycin	Streptomyces cyaneus
BGC0000231.5	0.61	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.57	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001513.3	0.57	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001197.5	0.57	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001273.3	0.56	PKS	asperlactone	Aspergillus ochraceus
BGC0000204.5	0.54	PKS	benastatin A, benastatin B, BE-43767, TAN1532B	Streptomyces sp. A2991200

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000272.3	0.74	PKS	spore pigment	Streptomyces collinus
BGC0000271.5	0.71	PKS	spore pigment	Streptomyces avermitilis
BGC0000215.5	0.69	PKS	curamycin	Streptomyces cyaneus
BGC0000231.5	0.61	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.57	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001513.3	0.57	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001197.5	0.57	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001273.3	0.56	PKS	asperlactone	Aspergillus ochraceus
BGC0000204.5	0.54	PKS	benastatin A, benastatin B, BE-43767, TAN1532B	Streptomyces sp. A2991200

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 23.42 out of 27.04

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 25.16 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 26.03 out of 30.11

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.88 out of 23.79

Confidence: medium

Additional weak hits (18):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.41 out of 25.75

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.24 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.06 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.66 out of 27.78

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.68 out of 27.04

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.02 out of 18.57

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.28 out of 36.53

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.04 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.23 out of 29.38

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.23 out of 39.62

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.45 out of 39.62

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.32 out of 30.98

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.86 out of 23.43

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.31 out of 26.85

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.08 out of 29.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.83 out of 29.65

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.44 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.46 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

Type II PKS

TFBS Finder

Type II PKS gene cluster prediction details

Cluster section:: 497660 to 570155
Starter unit(s):: acetyl-CoA (Score: 0.0; E-value: 0)
Product class(es):: pentangular polyphenol
tetracenomycin

IE257_RS10480: OXY (Score: 280.1; E-value: 8.4e-86)
IE257_RS10490: CYC C5-C18 (Score: 214.5; E-value: 9.2e-67)
IE257_RS10495: KS (Score: 713.8; E-value: 1.4e-217)
IE257_RS10500: CLF (Score: 551.4; E-value: 2.7e-168)
IE257_RS10505: ACP (Score: 55.6; E-value: 9.6e-18)
IE257_RS10510: CYC C9-C14 (Score: 242.6; E-value: 2.9e-75)
IE257_RS10515: CYC C4-C21/C2-C23|C2-C19 (Score: 150.9; E-value: 2.3e-47)

TFBS definitions

Regulator	Description
CelR	Cellobiose uptake repressor
DasR	N-acetylglucosamine dependent repressor
IolR	Regulator of the inositol catabolism

NZ_BMVL01000003.1 - Region 4 - ectoine

Download region GenBank file

Download region SVG

Location: 630,150 - 640,554 nt. (total: 10,405 nt) Show pHMM detection rules used

ectoine: ectoine_synt

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

IE257_RS10990: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000853.3	0.84	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.84	other (ectoine)	ectoine	Streptomyces sp.
BGC0000852.5	0.70	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.67	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.66	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.66	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.66	other (other)	ectoine	Methylomicrobium kenyense
BGC0000857.3	0.63	other (other)	ectoine	Methylophaga alcalica
BGC0000860.3	0.63	other (other)	ectoine	Methylarcula marina
BGC0000854.3	0.62	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000853.3	0.84	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.84	other (ectoine)	ectoine	Streptomyces sp.
BGC0000852.5	0.70	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.67	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.66	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.66	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.66	other (other)	ectoine	Methylomicrobium kenyense
BGC0000857.3	0.63	other (other)	ectoine	Methylophaga alcalica
BGC0000860.3	0.63	other (other)	ectoine	Methylarcula marina
BGC0000854.3	0.62	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

Additional weak hits (9):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.54 out of 39.62

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.88 out of 21.55

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.17 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.27 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.82 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.20 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.71 out of 26.06

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.11 out of 25.75

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.93 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000004.1 - Region 1 - hydrogen-cyanide

Download region GenBank file

Download region SVG

Location: 1 - 12,221 nt. (total: 12,221 nt) Show pHMM detection rules used

Region on contig edge.

hydrogen-cyanide: (Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase reductase

IE257_RS11170: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

IE257_RS11180: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

Hydrolases: deaminase peptidase

IE257_RS11160: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

IE257_RS11200: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002345.2	0.54	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0002436.2	0.37	other (other)	coformycin	Streptomyces citricolor
BGC0002285.2	0.35	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001007.5	0.31	NRPS, PKS	lymphostin, neolymphostinol B, lymphostinol, neolymphostin b	Salinispora arenicola CNS-205
BGC0001006.5	0.30	NRPS, PKS	lymphostin, neolymphostinol B, lymphostinol, neolymphostin B	Salinispora tropica CNB-440
BGC0000595.3	0.29	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000971.5	0.26	NRPS, PKS	cinnabaramide A	Streptomyces cinnabarigriseus
BGC0001041.4	0.25	NRPS, PKS	salinosporamide A	Salinispora tropica CNB-440
BGC0002588.2	0.24	other (other)	enduracididine	uncultured bacterium
BGC0000135.5	0.22	PKS	reveromycin A	Streptomyces sp. SN-593

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002345.2	0.54	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0002436.2	0.37	other (other)	coformycin	Streptomyces citricolor
BGC0002285.2	0.35	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001007.5	0.31	NRPS, PKS	lymphostin, neolymphostinol B, lymphostinol, neolymphostin b	Salinispora arenicola CNS-205
BGC0001006.5	0.30	NRPS, PKS	lymphostin, neolymphostinol B, lymphostinol, neolymphostin B	Salinispora tropica CNB-440
BGC0000595.3	0.29	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000971.5	0.26	NRPS, PKS	cinnabaramide A	Streptomyces cinnabarigriseus
BGC0001041.4	0.25	NRPS, PKS	salinosporamide A	Salinispora tropica CNB-440
BGC0002588.2	0.24	other (other)	enduracididine	uncultured bacterium
BGC0000135.5	0.22	PKS	reveromycin A	Streptomyces sp. SN-593

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (7):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.57 out of 26.33

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.80 out of 31.73

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.29 out of 33.14

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.01 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 16.96 out of 22.40

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 20.42 out of 22.40

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_BMVL01000004.1 - Region 2 - RiPP-like

Download region GenBank file

Download region SVG

Location: 71,563 - 82,900 nt. (total: 11,338 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000541.5	0.33	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000351.5	0.33	NRPS	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0000565.5	0.31	ribosomal	goadsporin	Streptomyces sp. TP-A0584
BGC0001423.4	0.29	NRPS, PKS	myxochromide C	Myxococcus hansupus
BGC0001424.4	0.29	NRPS, PKS	myxochromide C	Myxococcus virescens
BGC0001421.4	0.29	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0001427.4	0.29	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001425.4	0.29	NRPS, PKS	myxochromide A	Myxococcus xanthus DK 1622
BGC0001426.4	0.29	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001428.4	0.29	NRPS, PKS	myxochromide A	Myxococcus xanthus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000541.5	0.33	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000351.5	0.33	NRPS	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0000565.5	0.31	ribosomal	goadsporin	Streptomyces sp. TP-A0584
BGC0001423.4	0.29	NRPS, PKS	myxochromide C	Myxococcus hansupus
BGC0001424.4	0.29	NRPS, PKS	myxochromide C	Myxococcus virescens
BGC0001421.4	0.29	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0001427.4	0.29	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001425.4	0.29	NRPS, PKS	myxochromide A	Myxococcus xanthus DK 1622
BGC0001426.4	0.29	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001428.4	0.29	NRPS, PKS	myxochromide A	Myxococcus xanthus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.56 out of 20.47

Confidence: medium

Additional weak hits (2):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 15.87 out of 30.97

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000004.1 - Region 3 - terpene

Download region GenBank file

Download region SVG

Location: 199,201 - 221,417 nt. (total: 22,217 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS11980: aldehyde oxidase and xanthine dehydrogenase

smcogs: SMCOG1293: aldehyde oxidase and xanthine dehydrogenase

Transferases: acetyltransferase

IE257_RS12005: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Lyases: cyclase

IE257_RS12015: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001972.4	0.44	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis
BGC0002120.3	0.27	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0000818.5	0.26	NRPS	notoamide A	Aspergillus versicolor
BGC0001060.5	0.22	PKS	dynemicin A	Micromonospora chersina
BGC0000949.5	0.18	NRPS	A-500359 A, A-500359 B	Streptomyces griseus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001972.4	0.44	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis
BGC0002120.3	0.27	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0000818.5	0.26	NRPS	notoamide A	Aspergillus versicolor
BGC0001060.5	0.22	PKS	dynemicin A	Micromonospora chersina
BGC0000949.5	0.18	NRPS	A-500359 A, A-500359 B	Streptomyces griseus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

IE257_RS12015: T1TS + T1TS

Domain 1 (2 - 325): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (326 - 706): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (8):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.67 out of 30.22

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.77 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.61 out of 30.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.11 out of 36.54

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.12 out of 29.65

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.92 out of 27.04

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.20 out of 32.93

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator

NZ_BMVL01000004.1 - Region 4 - T1PKS

Download region GenBank file

Download region SVG

Location: 269,503 - 315,868 nt. (total: 46,366 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

IE257_RS12255: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

IE257_RS12280: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: aminotransferase formyltransferase

IE257_RS12235: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

IE257_RS12245: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

IE257_RS12300: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

Lyases: dehydratase

IE257_RS12250: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

IE257_RS12225: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

IE257_RS12285: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

IE257_RS12290: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001164.3	0.67	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002191.2	0.64	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000357.5	0.63	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0002274.2	0.62	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001164.3	0.67	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002191.2	0.64	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000357.5	0.63	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0002274.2	0.62	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.31 out of 18.46

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.87 out of 23.79

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (6):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.70 out of 26.85

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 18.93 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.07 out of 27.78

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.31 out of 29.51

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.10 out of 28.19

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.44 out of 27.04

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 269502 - 315868:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS12285: mmal

PKS_AT (620..916): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 158.0
Ethylmalonyl-CoA: 148.6
Methoxymalonyl-CoA: 130.4
2-Methylbutyryl-CoA: 105.8
trans-1,2-CPDA: 97.4
Propionyl-CoA: 87.6
Isobutyryl-CoA: 84.5
Malonyl-CoA: 83.8
Benzoyl-CoA: 57.6
CHC-CoA: 55.7
Acetyl-CoA: 48.1
fatty_acid: 46.8
3-Methylbutyryl-CoA: 25.5
inactive: 0.0

IE257_RS12290: NH2

CAL_domain (51..500): NH2

minowa: NH2

Prediction, score:

NH2: 175.1
Acetyl-CoA: 55.7
fatty_acid: 38.5
AHBA: 10.2
shikimic_acid: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence

NZ_BMVL01000004.1 - Region 5 - terpene

Download region GenBank file

Download region SVG

Location: 557,888 - 584,881 nt. (total: 26,994 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS13520: dehydrogenase

smcogs: SMCOG1181: dehydrogenase

IE257_RS13560: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: aminotransferase methyltransferase transferase

IE257_RS13505: methyltransferase

smcogs: SMCOG1089: methyltransferase

IE257_RS13515: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

IE257_RS38185: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Ligases: synthetase

IE257_RS13565: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000663.5	0.67	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000656.5	0.51	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0002162.3	0.49	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0002610.3	0.49	terpene	stellatic acid	Aspergillus stellatus
BGC0000630.3	0.49	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000646.5	0.49	terpene	β-carotein	uncultured bacterium
BGC0000640.3	0.48	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000644.3	0.47	terpene	carotenoid	Dietzia sp. CQ4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000663.5	0.67	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000656.5	0.51	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0002162.3	0.49	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0002610.3	0.49	terpene	stellatic acid	Aspergillus stellatus
BGC0000630.3	0.49	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000646.5	0.49	terpene	β-carotein	uncultured bacterium
BGC0000640.3	0.48	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000644.3	0.47	terpene	carotenoid	Dietzia sp. CQ4

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

IE257_RS13545: PT_phytoene_like

Domain 1 (5 - 273): Prenyltransferase; Phytoene synthase like

Subtype: Hydroxysqualene synthase

Reactions:: PSPP → hydroxysqualene

IE257_RS13550: PT_phytoene_like

Domain 1 (16 - 315): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → diapophytoene; FPP → squalene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

IE257_RS13565: PT_FPPS_like

Domain 1 (94 - 389): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

IE257_RS13570: T2TS

Domain 1 (106 - 735): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.00 out of 20.47

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.64 out of 27.55

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.71 out of 23.79

Confidence: medium

Additional weak hits (6):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.67 out of 26.33

Confidence: weak

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 25.31

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.03 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.35 out of 30.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.73 out of 29.25

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
GnfM	Regulator of nitrogen-fixation genes
IolR	Regulator of the inositol catabolism
MexT	Global virulence regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000005.1 - Region 1 - T1PKS,hglE-KS

Download region GenBank file

Download region SVG

Location: 13,909 - 65,573 nt. (total: 51,665 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
hglE-KS: (hglE or hglD)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

IE257_RS13985: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IE257_RS14045: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: glycosyltransferase

IE257_RS14010: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: hydrolase

IE257_RS13950: isochorismatase

smcogs: SMCOG1027: isochorismatase

IE257_RS14015: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

IE257_RS14100: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: hydratase

IE257_RS13965: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: synthase

IE257_RS14030: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

IE257_RS14075: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

IE257_RS38905: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transporter

IE257_RS14110: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.58	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.57	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.57	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001164.3	0.53	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.53	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002192.3	0.50	PKS	FR901512	fungal sp. No.14919
BGC0001284.4	0.47	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000041.3	0.47	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.46	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.46	PKS	YWA1	Aspergillus oryzae RIB40

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.62	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.62	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001164.3	0.60	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002192.3	0.58	PKS	FR901512	fungal sp. No.14919
BGC0001284.4	0.57	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000041.3	0.57	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.56	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.56	PKS	YWA1	Aspergillus oryzae RIB40

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (13):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.32 out of 26.85

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.23 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.52 out of 36.54

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.41 out of 19.26

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.44 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.34 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.98 out of 29.65

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.04 out of 18.57

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.81 out of 28.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.26 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 20.13 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.06 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

IE257_RS38905: mal

PKS_AT (653..864): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 16.3
inactive: 9.5
Methoxymalonyl-CoA: 9.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

IE257_RS14030: mal

PKS_AT (1214..1502): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 65.9
Methoxymalonyl-CoA: 37.1
Methylmalonyl-CoA: 22.7
Propionyl-CoA: 20.3
CHC-CoA: 8.6
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.7

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_BMVL01000005.1 - Region 2 - NRPS

Download region GenBank file

Download region SVG

Location: 98,041 - 185,101 nt. (total: 87,061 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase electron transfer

IE257_RS14275: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IE257_RS14500: flavodoxin

smcogs: SMCOG1134: flavodoxin

Hydrolases: amidase deacetylase

IE257_RS14325: amidase

smcogs: SMCOG1105: amidase

IE257_RS14490: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

Lyases: lyase

IE257_RS14350: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Ligases: ligase synthase

IE257_RS14345: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS14360: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

IE257_RS14370: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS14375: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS14380: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS14385: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS14425: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS14430: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002095.3	0.84	NRPS	leupeptin Pr, leupeptin Ac	Streptomyces roseus
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.76	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.74	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002095.3	0.84	NRPS	leupeptin Pr, leupeptin Ac	Streptomyces roseus
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.76	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.74	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.36 out of 23.79

Confidence: medium

Additional weak hits (15):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 17.57 out of 25.88

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.41 out of 25.75

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.84 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.18 out of 29.38

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 17.67 out of 22.40

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.17 out of 26.12

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.52 out of 29.25

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 17.76 out of 25.88

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.46 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.77 out of 33.14

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.46 out of 32.93

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.59 out of 24.78

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.49 out of 26.85

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.33 out of 20.01

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 98040 - 185101:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - Ser) + (Leu - X - Gly) + (X) + (X) + (Thr - Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS14345: Thr - Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (508..960): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGNQVFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1648..2070): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAVDVTY
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS14375: X

AMP-binding (471..868): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDRSFDASTYEVFLMTGGETNAYGPTETTTFAAT
Nearest Stachelhaus code(s):: Leu or Ile DALFMGATFK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

IE257_RS14380: X

AMP-binding (490..903): X

nrpys: Dab, Dpr

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HAKAFDISVWQAETIVNGEVNAYGLTECSDDNTH
Nearest Stachelhaus code(s):: Dpr DIWEMTADDK (85% 8Å match); Dab DIWELTADDK (68% 8Å match)
Stachelhaus code match:: 80% (moderate)

IE257_RS14385: Arg

Search NORINE for peptide: strict or relaxed

AMP-binding (14..419): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QTLAFDVCLEEPNLVIGSDVSCYGLTESTITAAS
Nearest Stachelhaus code(s):: Arg DVENVGGITK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)

IE257_RS14425: Leu - X - Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (519..923): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDASFDASTYEVFLWTGGEVNVYGPTEATTFATR
Nearest Stachelhaus code(s):: Leu DAYFWGVTFK (91% 8Å match); Val DAYFWGVTFK (88% 8Å match); Ile or Val DAYFWGVTFK (85% 8Å match); Ile DAYFWGVTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1590..1999): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YALTFDPSVRDASIMTTGEVNQYGPTECTNTTTY
Nearest Stachelhaus code(s):: Glu DPRSLSQMAK (71% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (2671..3092): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FDMTFDIAVLEVQALCGGEWNVYGPTETTVWSTA
Nearest Stachelhaus code(s):: Gly DILQLGVVWK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS14430: X - Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (460..859): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGHAFDVSVLDGDLSTGGEWNAYGPTEAAVSVTM
Nearest Stachelhaus code(s):: Glu DVLKVGAVNK (59% 8Å match); Phe or Trp DVLSIGAVVK (53% 8Å match); Leu DALHSGAVAK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2009..2404): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAVDVTH
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
IolR	Regulator of the inositol catabolism

NZ_BMVL01000005.1 - Region 3 - terpene

Download region GenBank file

Download region SVG

Location: 433,327 - 454,424 nt. (total: 21,098 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

IE257_RS15735: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acetyltransferase methyltransferase

IE257_RS15705: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

IE257_RS15745: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase

IE257_RS15740: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000659.3	0.56	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000948.5	0.45	other (other)	α-galactosylceramide	Bacteroides fragilis NCTC 9343
BGC0001910.4	0.43	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001197.5	0.43	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001911.3	0.39	terpene	raimonol	Streptomyces anulatus
BGC0001483.4	0.39	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0001840.2	0.38	NRPS, PKS	detoxin S1	Streptomyces sp. NRRL S-325
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0002582.3	0.35	NRPS	guanipiperazine A, guanipiperazine B	Streptomyces chrestomyceticus
BGC0002246.3	0.35	terpene	naematolin	Hypholoma sublateritium FD-334 SS-4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000659.3	0.56	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000948.5	0.45	other (other)	α-galactosylceramide	Bacteroides fragilis NCTC 9343
BGC0001910.4	0.43	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001197.5	0.43	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001911.3	0.39	terpene	raimonol	Streptomyces anulatus
BGC0001483.4	0.39	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0001840.2	0.38	NRPS, PKS	detoxin S1	Streptomyces sp. NRRL S-325
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0002582.3	0.35	NRPS	guanipiperazine A, guanipiperazine B	Streptomyces chrestomyceticus
BGC0002246.3	0.35	terpene	naematolin	Hypholoma sublateritium FD-334 SS-4

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

IE257_RS15740: T1TS

Domain 1 (10 - 319): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.30 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.35 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.38 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description

NZ_BMVL01000005.1 - Region 4 - lanthipeptide-class-iii,terpene-precursor

Download region GenBank file

Download region SVG

Location: 539,615 - 571,517 nt. (total: 31,903 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)
terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: reductase

IE257_RS16325: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

Lyases: decarboxylase

IE257_RS16200: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: synthetase

IE257_RS16280: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	0.56	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000496.5	0.36	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000516.4	0.27	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000513.3	0.27	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.27	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.27	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000518.3	0.26	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0001210.5	0.26	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000527.5	0.24	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000553.3	0.24	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	0.82	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000496.5	0.69	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000513.3	0.61	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000501.3	0.60	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000518.3	0.60	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000553.3	0.60	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000546.3	0.54	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0002700.2	0.51	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002698.2	0.51	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for IE257_RS16235

IE257_RS16230

Leader:

MTLLDLQSMDTPKEETTEAAH

Core:

GGGGGSRASLLLCGDSSLSITTCN

Core with dehydrations:

GGGGGDhaRADhaLLLCysGDDhaDhaLDhaIDhbDhbCysN

antiSMASH-DB 4.0 matches for IE257_RS16230

100.0%

NZ_BMVE01000003.1and 16 others:

NZ_BMVL01000005.1 Lanthipeptides: IE257_RS16230_lanthipeptideNZ_CP011664.1 Lanthipeptides: M444_RS30240_lanthipeptideNZ_CP020039.1 Lanthipeptides: B4U39_RS34445_lanthipeptideNZ_CP020555.1 Lanthipeptides: B6R96_RS03515_lanthipeptideNZ_CP029189.1 Lanthipeptides: DEJ51_RS31910_lanthipeptideNZ_CP030286.1 Lanthipeptides: DRB89_RS16930_lanthipeptideNZ_CP033581.1 Lanthipeptides: EES41_RS31595_lanthipeptideNZ_CP034350.1 Lanthipeptides: D1J60_RS32970_lanthipeptideNZ_CP115936.1 Lanthipeptides: PET44_RS28650_lanthipeptideNZ_JAFLQY010000003.1 Lanthipeptides: J0X20_RS29030_lanthipeptideNZ_JAINUL010000001.1 Lanthipeptides: K7B10_RS06210_lanthipeptideNZ_JANAVF010000017.1 Lanthipeptides: NLX94_RS31205_lanthipeptideNZ_LIVP01000027.1 Lanthipeptides: AMK15_RS28680_lanthipeptideNZ_LWLD01000038.1 Lanthipeptides: A6A28_RS28600_lanthipeptideNZ_ML123038.1 Lanthipeptides: EES37_RS30340_lanthipeptideNZ_NNBM01000002.1 Lanthipeptides: CG724_RS07855_lanthipeptide

Lanthipeptides: IE248_RS10435_lanthipeptide

1

GGGGGSRASLLLCGDSSLSITTCN

24

GGGGGSRASLLLCGDSSLSITTCN

1

GGGGGSRASLLLCGDSSLSITTCN

24

91.7%

NZ_JACHJG010000001.1:

Lanthipeptides: FHS38_RS02665_lanthipeptide

1

GGGGGSRASLLLCGDSSLSITTCN

24

GGGGGSRASLLLCGDSSLS+ TCN

1

GGGGGSRASLLLCGDSSLSVVTCN

24

83.3%

NZ_BBOK01000011.1and 28 others:

NZ_BEWD01000001.1 Lanthipeptides: Sru02f_RS01660_lanthipeptideNZ_BMSL01000016.1 Lanthipeptides: IE215_RS23330_lanthipeptideNZ_BMUC01000023.1 Lanthipeptides: IE172_RS32210_lanthipeptideNZ_BMVQ01000005.1 Lanthipeptides: IE283_RS17035_lanthipeptideNZ_BMWI01000025.1 Lanthipeptides: IE253_RS33945_lanthipeptideNZ_CM002280.1 Lanthipeptides: M877_RS44575_lanthipeptideNZ_CP012382.1 Lanthipeptides: SAM23877_RS30085_lanthipeptideNZ_CP012949.1 Lanthipeptides: SAM40697_RS29010_lanthipeptideNZ_CP018047.1 Lanthipeptides: BBN63_RS03065_lanthipeptideNZ_CP042324.1 Lanthipeptides: FQ762_RS34495_lanthipeptideNZ_CP043959.1 Lanthipeptides: F3L20_RS19105_lanthipeptideNZ_CP045547.1 Lanthipeptides: GEV49_RS05730_lanthipeptideNZ_CP048397.1 Lanthipeptides: SSPS47_RS31455_lanthipeptideNZ_CP054939.1 Lanthipeptides: HUT14_RS29815_lanthipeptideNZ_CP094918.1 Lanthipeptides: MTR79_RS16715_lanthipeptideNZ_GG657756.1 Lanthipeptides: SSPG_RS05020_lanthipeptideNZ_GG657758.1 Lanthipeptides: SSRG_RS03855_lanthipeptideNZ_JAAIFS010000001.1 Lanthipeptides: GUR47_RS02255_lanthipeptideNZ_JACHEM010000005.1 Lanthipeptides: HNQ79_RS11445_lanthipeptideNZ_KZ195574.1 Lanthipeptides: B9W62_RS05695_lanthipeptideNZ_LT629768.1 Lanthipeptides: BLU60_RS34110_lanthipeptideNZ_MQUH01000025.1 Lanthipeptides: BSZ07_RS33280_lanthipeptideNZ_RAII01000001.1 Lanthipeptides: EBH12_RS10360_lanthipeptideNZ_RSEG01000006.1 Lanthipeptides: EIA57_RS12710_lanthipeptideNZ_SOAV01000001.1 Lanthipeptides: EV562_RS04520_lanthipeptideNZ_VIUV01000020.1 Lanthipeptides: FB570_RS34060_lanthipeptideNZ_WHZR01000001.1 Lanthipeptides: STPH1_RS04005_lanthipeptideNZ_WXVT01000001.1 Lanthipeptides: STPH2_RS03995_lanthipeptide

Lanthipeptides: APK71_RS25550_lanthipeptide

5

GSRASLLLCGDSSLSITTCN

24

GSRASLLLCGDSSLSITTCN

2

GSRASLLLCGDSSLSITTCN

21

with 100 more sequence matches

Only the first 10 are displayed here.

83.3%

NZ_CP102509.1:

Lanthipeptides: JIW86_RS37035_lanthipeptide

3

GGGSRASLLLCGDSSLSITTCN

24

GGGSRASLLLCGDSSLS+TTC+

2

GGGSRASLLLCGDSSLSLTTCH

23

83.3%

NZ_JAGGOI010000031.1:

Lanthipeptides: J7E87_RS22160_lanthipeptide

4

GGSRASLLLCGDSSLSITTCN

24

GGSRASLLLCGDSSLS+TTCN

1

GGSRASLLLCGDSSLSVTTCN

21

81.5%

NZ_CP023701.1and 2 others:

NZ_CP043638.1 Lanthipeptides: F1D59_RS34930_lanthipeptideNZ_CP116355.1 Lanthipeptides: KPP03845_RS32700_lanthipeptide

Lanthipeptides: CP968_RS29065_lanthipeptide

3

GGGSRASLLLCGDSSLSITTCN

24

GGGSRASLLLCGDSSLSITTCN

6

GGGSRASLLLCGDSSLSITTCN

27

79.2%

NC_018750.1and 77 others:

NC_018750.1 Lanthipeptides: SVEN_RS32110_lanthipeptideNZ_BBNO01000003.1 Lanthipeptides: TU31_RS37940_lanthipeptideNZ_BMSV01000002.1 Lanthipeptides: IE106_RS06150_lanthipeptideNZ_BMTO01000007.1 Lanthipeptides: IE136_RS15615_lanthipeptideNZ_BMTP01000009.1 Lanthipeptides: IE109_RS18470_lanthipeptideNZ_BMTU01000009.1 Lanthipeptides: IE118_RS22890_lanthipeptideNZ_BMUN01000001.1 Lanthipeptides: IE238_RS00740_lanthipeptideNZ_CP007699.2 Lanthipeptides: T261_RS39185_lanthipeptideNZ_CP009754.1 Lanthipeptides: NI25_RS05015_lanthipeptideNZ_CP015866.1 Lanthipeptides: Spa2297_RS27800_lanthipeptideNZ_CP017157.1 Lanthipeptides: SL103_RS15895_lanthipeptideNZ_CP019457.1 Lanthipeptides: BXT88_RS03410_lanthipeptideNZ_CP019724.1 Lanthipeptides: B1H29_RS35355_lanthipeptideNZ_CP019779.1 Lanthipeptides: STRMOE7_RS03775_lanthipeptideNZ_CP023691.1 Lanthipeptides: CP981_RS03050_lanthipeptideNZ_CP023700.1 Lanthipeptides: CP969_RS04700_lanthipeptideNZ_CP027299.1 Lanthipeptides: C1N79_RS34970_lanthipeptideNZ_CP029042.1 Lanthipeptides: DDE74_RS03525_lanthipeptideNZ_CP029195.1 Lanthipeptides: DEJ45_RS04995_lanthipeptideNZ_CP029195.1 Lanthipeptides: DEJ45_RS04995_lanthipeptideNZ_CP029196.1 Lanthipeptides: DEJ44_RS30395_lanthipeptideNZ_CP029196.1 Lanthipeptides: DEJ44_RS30395_lanthipeptideNZ_CP029541.1 Lanthipeptides: DKG71_RS05245_lanthipeptideNZ_CP034279.1 Lanthipeptides: EIZ62_RS28660_lanthipeptideNZ_CP034353.1 Lanthipeptides: D1J63_RS04900_lanthipeptideNZ_CP039123.1 Lanthipeptides: E5N77_RS35250_lanthipeptideNZ_CP043317.1 Lanthipeptides: FYC74_RS09720_lanthipeptideNZ_CP046905.1 Lanthipeptides: GPZ77_RS32930_lanthipeptideNZ_CP047140.1 Lanthipeptides: GLX30_RS11420_lanthipeptideNZ_CP050976.1 Lanthipeptides: HEP86_RS04470_lanthipeptideNZ_CP054920.1 Lanthipeptides: HUT15_RS04615_lanthipeptideNZ_CP060825.1 Lanthipeptides: IAG43_RS32355_lanthipeptideNZ_CP074111.1 Lanthipeptides: KE639_RS31630_lanthipeptideNZ_CP084681.1 Lanthipeptides: KI385_RS00885_lanthipeptideNZ_CP085300.1 Lanthipeptides: LJA35_RS05220_lanthipeptideNZ_CP086217.1 Lanthipeptides: LJ741_RS03435_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS09690_lanthipeptideNZ_CP098437.1 Lanthipeptides: NCG97_RS34740_lanthipeptideNZ_CP099468.1 Lanthipeptides: NFX46_RS16770_lanthipeptideNZ_CP101137.1 Lanthipeptides: M6G08_RS24535_lanthipeptideNZ_CP102332.1 Lanthipeptides: NRO40_RS01140_lanthipeptideNZ_CP102609.1 Lanthipeptides: NUT86_RS03980_lanthipeptideNZ_CP104697.1 Lanthipeptides: N6Q81_RS33560_lanthipeptideNZ_CP114202.1 Lanthipeptides: STRLI_RS35870_lanthipeptideNZ_CP114203.1 Lanthipeptides: STRNI_RS37360_lanthipeptideNZ_CP114205.1 Lanthipeptides: STRTU_RS33380_lanthipeptideNZ_JACHJE010000006.1 Lanthipeptides: FHS32_RS15850_lanthipeptideNZ_JACHJI010000005.1 Lanthipeptides: FHS37_RS15430_lanthipeptideNZ_JACSCH020000032.1 Lanthipeptides: E0F75_RS38565_lanthipeptideNZ_JACSCH020000032.1 Lanthipeptides: E0F75_RS38565_lanthipeptideNZ_JACSCH020000032.1 Lanthipeptides: E0F75_RS38565_lanthipeptideNZ_JACWAA010000001.1 Lanthipeptides: ICA23_RS00800_lanthipeptideNZ_JADMLH010000003.1 Lanthipeptides: IT780_RS09875_lanthipeptideNZ_JAFEJA010000002.1 Lanthipeptides: JE024_RS34830_lanthipeptideNZ_JAGPYC010000033.1 Lanthipeptides: KBZ00_RS31440_lanthipeptideNZ_JAHLXF010000022.1 Lanthipeptides: KQY13_RS30555_lanthipeptideNZ_JAIHBQ010000001.1 Lanthipeptides: K4902_RS04515_lanthipeptideNZ_JAJJMU010000026.1 Lanthipeptides: LOC25_RS31275_lanthipeptideNZ_JAJNOJ010000002.1 Lanthipeptides: MBT42_RS06255_lanthipeptideNZ_JAJNOJ010000002.1 Lanthipeptides: MBT42_RS06255_lanthipeptideNZ_JAMGSL010000004.1 Lanthipeptides: M5362_RS09920_lanthipeptideNZ_JAMGSL010000004.1 Lanthipeptides: M5362_RS09920_lanthipeptideNZ_JAPKNL010000004.1 Lanthipeptides: OSI21_RS07750_lanthipeptideNZ_JH993790.1 Lanthipeptides: STRVIR_RS0131905_lanthipeptideNZ_KN039948.1 Lanthipeptides: FM21_RS29525_lanthipeptideNZ_LIPF01000004.1 Lanthipeptides: AMK11_RS16660_lanthipeptideNZ_LIVV01000002.1 Lanthipeptides: AMK27_RS08700_lanthipeptideNZ_LIVV01000002.1 Lanthipeptides: AMK27_RS08700_lanthipeptideNZ_LIVX01000004.1 Lanthipeptides: AMK29_RS15650_lanthipeptideNZ_LIWB01000010.1 Lanthipeptides: AMK33_RS24360_lanthipeptideNZ_LIYH01000004.1 Lanthipeptides: AMK26_RS17765_lanthipeptideNZ_MPOH02000012.1 Lanthipeptides: BM536_RS17150_lanthipeptideNZ_NBCN01000003.1 Lanthipeptides: BKD26_RS06570_lanthipeptideNZ_NNBP01000011.1 Lanthipeptides: CG747_RS19290_lanthipeptideNZ_NNBP01000011.1 Lanthipeptides: CG747_RS19290_lanthipeptideNZ_RAIF01000032.1 Lanthipeptides: EBH09_RS35480_lanthipeptideNZ_SOPG01000008.1 Lanthipeptides: E0I08_RS17090_lanthipeptide

Lanthipeptides: SVEN_RS32110_lanthipeptide

5

GSRASLLLCGDSSLSITTCN

24

GSRASLLLCGDSSLS+TTCN

2

GSRASLLLCGDSSLSVTTCN

21

79.2%

NZ_CP048835.1:

Lanthipeptides: C8250_RS00390_lanthipeptide

4

GGSRASLLLCGDSSLSITTCN

24

GGSRASLLLCG+SSLS+TTCN

1

GGSRASLLLCGNSSLSVTTCN

21

79.2%

NZ_QGGR01000008.1:

Lanthipeptides: BC793_RS19230_lanthipeptide

4

GGSRASLLLCGDSSLSITTCN

24

GGS+ASLLLCGDSSLS+TTCN

1

GGSQASLLLCGDSSLSVTTCN

21

77.8%

NZ_CP020042.1and 9 others:

NZ_CP023202.1 Lanthipeptides: SXIN_RS23100_lanthipeptideNZ_CP023692.1 Lanthipeptides: CP980_RS04735_lanthipeptideNZ_CP028158.1 Lanthipeptides: C6361_RS04660_lanthipeptideNZ_CP028159.1 Lanthipeptides: C6W10_RS01185_lanthipeptideNZ_FNHI01000003.1 Lanthipeptides: BLT82_RS06465_lanthipeptideNZ_JAGPXX010000007.1 Lanthipeptides: KBZ10_RS10785_lanthipeptideNZ_JAPNLM010000003.1 Lanthipeptides: OTB16_RS07305_lanthipeptideNZ_LIVQ01000005.1 Lanthipeptides: AMK16_RS35205_lanthipeptideNZ_LIVQ01000005.1 Lanthipeptides: AMK16_RS35205_lanthipeptide

Lanthipeptides: B4U46_RS32710_lanthipeptide

3

GGGSRASLLLCGDSSLSITTCN

24

GGGSRASLLLCGDSSLS+TTCN

6

GGGSRASLLLCGDSSLSVTTCN

27

75.0%

NZ_BNCA01000002.1and 1 other:

NZ_CP049782.1 Lanthipeptides: G7Z12_RS34445_lanthipeptide

Lanthipeptides: IE314_RS02760_lanthipeptide

5

GSRASLLLCGDSSLSITTCN

24

GSRASLLLCGDSSLS++TCN

2

GSRASLLLCGDSSLSVSTCN

21

75.0%

NZ_CP007574.1and 6 others:

NZ_CP007574.1 Lanthipeptides: DC74_RS36920_lanthipeptideNZ_CP026094.1 Lanthipeptides: SE95_RS37250_lanthipeptideNZ_CP104098.1 Lanthipeptides: N1H47_RS36625_lanthipeptideNZ_CP104098.1 Lanthipeptides: N1H47_RS36625_lanthipeptideNZ_CP119144.1 Lanthipeptides: P0D74_RS03260_lanthipeptideNZ_CP119144.1 Lanthipeptides: P0D74_RS03260_lanthipeptide

Lanthipeptides: DC74_RS36920_lanthipeptide

5

GSRASLLLCGDSSLSITTCN

24

GSRASLLLCGDSSLS+TTC

5

GSRASLLLCGDSSLSVTTCK

24

75.0%

NZ_CP036455.1:

Lanthipeptides: EKD16_RS17975_lanthipeptide

4

GGSRASLLLCGDSSLSITTCN

24

GS ASLLLCGDSSLS+TTCN

1

AGSEASLLLCGDSSLSLTTCN

21

MIBiG 4.0 matches for IE257_RS16230

45.8%

BGC0000553.3 (SSGG_RS09925):

Lanthipeptide: SRO15-2212

5

GSRASLLLCGDSSLSITTCN

24

GS+ SLL+C SSLS+ C

2

GSQVSLLVCEYSSLSVVLCT

21

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

IE257_RS16280: PT_FPPS_like

Domain 1 (51 - 339): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 20.47

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (9):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.97 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.17 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.45 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.59 out of 28.14

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.37 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.55 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.73 out of 28.19

Confidence: weak

Lanthipeptides

Terpene

TFBS Finder

Core peptide predictions

IE257_RS16230 - Class III

Cleavage pHMM score: 21.10

RODEO score: 0

Monoisotopic mass: 2099.0 Da

Molecular weight: 2100.3 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2118.3 Da N = 1; 2136.3 Da N = 2; 2154.4 Da N = 3; 2172.4 Da N = 4; 2190.4 Da N = 5

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000006.1 - Region 1 - NRPS-like,terpene

Download region GenBank file

Download region SVG

Location: 3,187 - 49,760 nt. (total: 46,574 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

IE257_RS16520: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IE257_RS16530: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: PPTase formyltransferase methyltransferase

IE257_RS16370: methyltransferase

smcogs: SMCOG1248: methyltransferase

IE257_RS16415: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

IE257_RS16450: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

Hydrolases: hydrolase

IE257_RS38310: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: cyclase dehydratase hydratase

IE257_RS16460: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

IE257_RS16515: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

IE257_RS16535: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

IE257_RS16455: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS16465: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS16470: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

IE257_RS16475: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000683.3	1.20	terpene	avermitilol	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001746.3	0.95	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0002631.2	0.42	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0000382.5	0.42	NRPS	lipstatin	Streptomyces toxytricini
BGC0002127.3	0.32	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0000674.5	0.32	terpene	(-)-δ-cadinene	Streptomyces clavuligerus ATCC 27064
BGC0000902.4	0.31	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0001605.3	0.28	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001223.5	0.28	other (other)	hydroxysporine	uncultured bacterium
BGC0002135.2	0.27	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000683.3	0.84	terpene	avermitilol	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001746.3	0.77	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0000382.5	0.64	NRPS	lipstatin	Streptomyces toxytricini
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0000342.4	0.60	NRPS	enniatin	Fusarium equiseti
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001240.4	0.59	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

IE257_RS16515: T1TS

Domain 1 (6 - 314): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.63 out of 18.46

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.79 out of 25.31

Confidence: medium

Additional weak hits (14):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.23 out of 26.85

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.01 out of 29.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.17 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.79 out of 29.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.34 out of 31.73

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.93 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.00 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.80 out of 24.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.52 out of 29.65

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.41 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.56 out of 32.93

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.11 out of 25.88

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.14 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Terpene

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 3186 - 49760:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 3186 - 46441:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS16455: X

AMP-binding (467..887): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FWMSFDISCFPLALLLGGEINAYGPTEATVAVLA
Nearest Stachelhaus code(s):: Leu DIFALGGVAK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

IE257_RS16465: X

AMP-binding (26..482): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IQIL----FYGDFAVSGGAHVTYGATEVLPITSI
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
RpoN	Regulator of nitrogen assimilation and virulence

NZ_BMVL01000006.1 - Region 2 - NRP-metallophore,NRPS

Download region GenBank file

Download region SVG

Location: 342,946 - 409,546 nt. (total: 66,601 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

IE257_RS18135: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

IE257_RS18225: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

IE257_RS18320: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

Transferases: formyltransferase methyltransferase

IE257_RS18140: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

IE257_RS18255: methyltransferase

smcogs: SMCOG1089: methyltransferase

IE257_RS18335: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: ligase

IE257_RS18235: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.72	NRPS	enniatin	Fusarium equiseti
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.04 out of 18.57

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.93 out of 26.33

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.39 out of 36.53

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.57 out of 36.53

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 24.10 out of 29.72

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.42 out of 20.47

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

Additional weak hits (27):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.05 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.88 out of 29.38

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.78 out of 39.62

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.94 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.40 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.67 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 22.12 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.89 out of 27.78

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.95 out of 21.55

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.39 out of 25.88

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.23 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.56 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.91 out of 27.78

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.71 out of 39.62

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.11 out of 29.51

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.24 out of 32.74

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 19.95 out of 30.98

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.89 out of 33.14

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.84 out of 28.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.33 out of 33.14

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.67 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 342945 - 409546:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Tyr - NMe-Phe)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS18235: Tyr - Phe

Search NORINE for peptide: strict or relaxed

AMP-binding (455..854): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDATVSETTLVVAGEINAYGPTEVSVCATM
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1522..1921): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: VLQAFDSAVWEFTLIVAGEINGYGPTEATVSATM
Nearest Stachelhaus code(s):: His DTWTIASVSK (74% 8Å match); Phe DAWTIAGVCK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
DmdR1	Iron(II)-dependent repressor
NtrC	Nitrogen regulatory protein C
ToxT	Regulator of MSHA biosynthesis
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000008.1 - Region 1 - terpene

Download region GenBank file

Download region SVG

Location: 261,638 - 282,267 nt. (total: 20,630 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

IE257_RS22770: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

IE257_RS22785: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IE257_RS22790: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Transferases: acetyltransferase

IE257_RS22845: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000888.5	0.45	other (other)	bacilysin	Bacillus sp. CS93
BGC0000867.3	0.44	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0001928.2	0.43	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000231.5	0.41	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001184.4	0.41	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000234.5	0.41	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001458.4	0.40	other (aminocoumarin)	salinipostin G, Salinipostin G, Salinipostin A, Salinipostin B, Salinipostin C, Salinipostin D, Salinipostin E, Salinipostin F, Salinipostin H, Salinipostin I, Salinipostin J, Salinipostin K	Salinispora tropica CNB-440
BGC0001197.5	0.39	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000188.5	0.39	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001343.3	0.38	PKS	aurachin A, aurachin D	Stigmatella aurantiaca Sg a15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000888.5	0.45	other (other)	bacilysin	Bacillus sp. CS93
BGC0000867.3	0.44	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0001928.2	0.43	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000231.5	0.41	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001184.4	0.41	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000234.5	0.41	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001458.4	0.40	other (aminocoumarin)	salinipostin G, Salinipostin G, Salinipostin A, Salinipostin B, Salinipostin C, Salinipostin D, Salinipostin E, Salinipostin F, Salinipostin H, Salinipostin I, Salinipostin J, Salinipostin K	Salinispora tropica CNB-440
BGC0001197.5	0.39	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000188.5	0.39	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001343.3	0.38	PKS	aurachin A, aurachin D	Stigmatella aurantiaca Sg a15

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

IE257_RS22795: TS_Pyr4

Domain 1 (1 - 161): Pyr4 type terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.45 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description

NZ_BMVL01000010.1 - Region 1 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 221,041 - 242,084 nt. (total: 21,044 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS25815: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Ligases: synthetase

IE257_RS25760: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000657.5	0.44	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000677.5	0.41	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001911.3	0.37	terpene	raimonol	Streptomyces anulatus
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001456.3	0.36	terpene	isorenieratene	Streptomyces argillaceus
BGC0000678.5	0.35	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000664.5	0.35	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000644.3	0.35	terpene	carotenoid	Dietzia sp. CQ4
BGC0000648.3	0.34	terpene	carotenoid	Myxococcus xanthus
BGC0000665.5	0.34	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000657.5	0.44	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000677.5	0.41	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001911.3	0.37	terpene	raimonol	Streptomyces anulatus
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001456.3	0.36	terpene	isorenieratene	Streptomyces argillaceus
BGC0000678.5	0.35	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000664.5	0.35	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000644.3	0.35	terpene	carotenoid	Dietzia sp. CQ4
BGC0000648.3	0.34	terpene	carotenoid	Myxococcus xanthus
BGC0000665.5	0.34	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

IE257_RS25760: PT_FPPS_like

Domain 1 (60 - 343): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.99 out of 20.47

Confidence: medium

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.59 out of 25.75

Confidence: strong

Additional weak hits (2):

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.96 out of 24.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.67 out of 20.01

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
MatP	Cell cycle regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000013.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 24,783 nt. (total: 24,783 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

IE257_RS28470: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

IE257_RS28480: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

IE257_RS28485: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

IE257_RS28495: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

Transferases: PPTase

IE257_RS28505: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

Ligases: ligase

IE257_RS28465: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS28490: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.71	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.68	NRPS	AM-toxin	Alternaria alternata
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0001825.4	0.66	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.71	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.68	NRPS	AM-toxin	Alternaria alternata
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0001825.4	0.66	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.06 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.97 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.98 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 24783:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS28465: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (451..849): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTVTSIKVVVGGEVNEYGPTEAVVGCCV
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS28490: fatty_acid

CAL_domain (39..467): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 39.8
AHBA: 25.7
NH2: 23.9
shikimic_acid: 12.2
Acetyl-CoA: 9.5

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_BMVL01000013.1 - Region 2 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 45,626 - 71,870 nt. (total: 26,245 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

IE257_RS28635: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

IE257_RS28645: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Translocases: permease

IE257_RS28670: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000552.3	0.57	ribosomal	SmbA, SmbB	Streptococcus mutans
BGC0000497.3	0.55	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0002110.3	0.53	ribosomal	birimositide	Streptomyces rimosus subsp. rimosus WC-3908
BGC0001311.5	0.52	ribosomal	flavecins	Ruminococcus flavefaciens FD-1
BGC0000517.4	0.51	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0001229.4	0.51	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0002005.2	0.51	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0001391.3	0.51	ribosomal	thusin	Bacillus thuringiensis serovar rongseni
BGC0000554.4	0.50	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001210.5	0.50	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000552.3	0.57	ribosomal	SmbA, SmbB	Streptococcus mutans
BGC0000497.3	0.55	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0002110.3	0.53	ribosomal	birimositide	Streptomyces rimosus subsp. rimosus WC-3908
BGC0001311.5	0.52	ribosomal	flavecins	Ruminococcus flavefaciens FD-1
BGC0000517.4	0.51	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0001229.4	0.51	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0002005.2	0.51	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0001391.3	0.51	ribosomal	thusin	Bacillus thuringiensis serovar rongseni
BGC0000554.4	0.50	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001210.5	0.50	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for IE257_RS28635

IE257_RS28640

Leader:

MSNIVKDATTGFVSEEELVQLAEEPSELAAGAAASGILCGAGITTAITTFSAAMQGG

Core:

CPTSGCTGRC

Core with dehydrations:

CysPDhbDhaGCysDhbGRCys

antiSMASH-DB 4.0 matches for IE257_RS28640

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: IE257_RS28640_lanthipeptide

Lanthipeptides: IE257_RS28640_lanthipeptide

1

CPTSGCTGRC

10

CPTSGCTGRC

1

CPTSGCTGRC

10

72.7%

NZ_GG657754.1and 1 other:

NZ_GG657754.1 Lanthipeptides: SSOG_RS27835_lanthipeptide

Lanthipeptides: SSOG_RS27835_lanthipeptide

1

CPTSGCTGRC

10

CPTSGCT +C

1

CPTSGCTSKC

10

50.0%

NZ_CP061033.1and 1 other:

NZ_CP061033.1 Lanthipeptides: IAU67_RS09325_lanthipeptide

Lanthipeptides: IAU67_RS09325_lanthipeptide

1

CPTSGCTGRC

10

CPTSGCT C

7

CPTSGCTHSC

16

with 53 more sequence matches

Only the first 10 are displayed here.

50.0%

NZ_NTSF01000026.1and 1 other:

NZ_NTSF01000026.1 Lanthipeptides: CN325_RS31015_lanthipeptide

Lanthipeptides: CN325_RS31015_lanthipeptide

1

CPTSGCTGRC

10

CPTS CT RC

3

CPTSKCTTRC

12

46.2%

NZ_BJTV01000004.1and 5 others:

NZ_BJTV01000004.1 Lanthipeptides: TNCT1_RS35800_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptide

Lanthipeptides: TNCT1_RS35800_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT C

4

CPTTKCTSAC

13

35.3%

NZ_QKTG01000020.1and 1 other:

NZ_QKTG01000020.1 Lanthipeptides: DEI99_RS08060_lanthipeptide

Lanthipeptides: DEI99_RS08060_lanthipeptide

1

CPTSGCTGRC

10

CPT CT +C

8

CPTGACTSKC

17

33.3%

NZ_CP115450.1and 1 other:

NZ_CP115450.1 Lanthipeptides: O1G21_RS35405_lanthipeptide

Lanthipeptides: O1G21_RS35405_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT RC

12

CPTTACTSRC

21

26.1%

NZ_CP050134.2and 3 others:

NZ_EQ973330.1 Lanthipeptides: CORMATOL_RS14310_lanthipeptideNZ_LR134504.1 Lanthipeptides: EL293_RS13085_lanthipeptideNZ_LR134504.1 Lanthipeptides: EL293_RS13085_lanthipeptide

Lanthipeptides: HBA49_RS00775_lanthipeptide

1

CPTSGCTGRC

10

CPT+ C+ RC

14

CPTTRCSRRC

23

25.0%

NZ_FQZK01000006.1and 2 others:

NZ_FQZK01000006.1 Lanthipeptides: BUE45_RS13105_lanthipeptideNZ_FQZK01000006.1 Lanthipeptides: BUE45_RS13105_lanthipeptide

Lanthipeptides: BUE45_RS13105_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT RC

19

CPTTVCTSRC

28

25.0%

NZ_LR134483.1and 1 other:

NZ_LR134483.1 Lanthipeptides: EL241_RS13590_lanthipeptide

Lanthipeptides: EL241_RS13590_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT +C

14

CPTAKCTSKC

23

24.2%

NZ_NTSF01000026.1and 1 other:

NZ_NTSF01000026.1 Lanthipeptides: CN325_RS21840_lanthipeptide

Lanthipeptides: CN325_RS21840_lanthipeptide

1

CPTSGCTGRC

10

CPTS CT RC

20

CPTSKCTERC

29

22.6%

NZ_AP026714.1and 21 others:

NZ_AP026714.1 Lanthipeptides: PO853_RS06520_lanthipeptideNZ_CP028724.1 Lanthipeptides: C7I31_RS11270_lanthipeptideNZ_CP028724.1 Lanthipeptides: C7I31_RS11270_lanthipeptideNZ_CP092907.1 Lanthipeptides: MK744_RS03990_lanthipeptideNZ_CP092907.1 Lanthipeptides: MK744_RS03990_lanthipeptideNZ_KB944759.1 Lanthipeptides: WOA_RS09815_lanthipeptideNZ_KB944759.1 Lanthipeptides: WOA_RS09815_lanthipeptideNZ_KZ845892.1 Lanthipeptides: EB42_RS00090_lanthipeptideNZ_KZ845892.1 Lanthipeptides: EB42_RS00090_lanthipeptideNZ_KZ845958.1 Lanthipeptides: EB53_RS07695_lanthipeptideNZ_KZ845958.1 Lanthipeptides: EB53_RS07695_lanthipeptideNZ_LR607361.1 Lanthipeptides: FN738_RS03740_lanthipeptideNZ_LR607361.1 Lanthipeptides: FN738_RS03740_lanthipeptideNZ_LR607378.1 Lanthipeptides: FN751_RS00125_lanthipeptideNZ_LR607378.1 Lanthipeptides: FN751_RS00125_lanthipeptideNZ_LSFS01000007.1 Lanthipeptides: AW733_RS07745_lanthipeptideNZ_LSFS01000007.1 Lanthipeptides: AW733_RS07745_lanthipeptideNZ_NGMP01000002.1 Lanthipeptides: A5830_RS04680_lanthipeptideNZ_NGMP01000002.1 Lanthipeptides: A5830_RS04680_lanthipeptideNZ_NGMV01000001.1 Lanthipeptides: A5824_RS07985_lanthipeptideNZ_NGMV01000001.1 Lanthipeptides: A5824_RS07985_lanthipeptide

Lanthipeptides: PO853_RS06520_lanthipeptide

1

CPTSGCTGRC

10

CPTS CT C

22

CPTSVCTRAC

31

22.2%

NZ_KE384533.1and 2 others:

NZ_KE384533.1 Lanthipeptides: K329_RS31315_lanthipeptideNZ_KE384533.1 Lanthipeptides: K329_RS31315_lanthipeptide

Lanthipeptides: K329_RS31315_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT C

18

CPTTSCTDEC

27

MIBiG 4.0 matches for IE257_RS28640

23.1%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

1

CPTSGCTGRC

10

CPT+ CT +C

17

CPTTKCTSKC

26

18.9%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

1

CPTSGCTGRC

10

CPT+ CT RC

28

CPTTKCTSRC

37

IE257_RS28650

Leader:

MAAGGKRPRESKKRSSLMHNRNVDILEEINEQNLDGLSAG

Core:

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQR

Core with dehydrations:

DhbIFEDhbVDhbYDhbVADhaWALGNNGHLCysDhbVDhbVECysQKNCysQR

antiSMASH-DB 4.0 matches for IE257_RS28650

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: IE257_RS28650_lanthipeptide

Lanthipeptides: IE257_RS28650_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQR

34

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQR

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQR

34

64.9%

NZ_CP115450.1and 1 other:

NZ_CP115450.1 Lanthipeptides: O1G21_RS35415_lanthipeptide

Lanthipeptides: O1G21_RS35415_lanthipeptide

3

FETVTYTVASWALGNNGHLCTVTVECQKNCQR

34

E +TYT ASWA+GNNG+LCTVT ECQKNCQ

6

IEVLTYTAASWAMGNNGYLCTVTAECQKNCQH

37

52.9%

NZ_CP043405.1and 1 other:

NZ_CP043405.1 Lanthipeptides: FY406_RS04355_lanthipeptide

Lanthipeptides: FY406_RS04355_lanthipeptide

2

IFETVTYTVASWALGNNGHLCTVTVECQKNCQ

33

I TV + S LGN G++CTVTVEC +NCQ

1

IGTTVVNSTFSIVLGNKGYICTVTVECMRNCQ

32

with 98 more sequence matches

Only the first 10 are displayed here.

50.0%

NC_018089.1and 11 others:

NC_018089.1 Lanthipeptides: SMUGS5_RS08725_lanthipeptideNZ_CP044493.1 Lanthipeptides: FSA40_RS09065_lanthipeptideNZ_CP044493.1 Lanthipeptides: FSA40_RS09065_lanthipeptideNZ_CP050270.1 Lanthipeptides: HB753_RS00325_lanthipeptideNZ_CP050270.1 Lanthipeptides: HB753_RS00325_lanthipeptideNZ_JADPAN010000004.1 Lanthipeptides: I4U28_RS03985_lanthipeptideNZ_JADPAN010000004.1 Lanthipeptides: I4U28_RS03985_lanthipeptideNZ_JALDUN010000005.1 Lanthipeptides: M1F82_RS08120_lanthipeptideNZ_JALDUN010000005.1 Lanthipeptides: M1F82_RS08120_lanthipeptideNZ_JAQCYD010000003.1 Lanthipeptides: PGX70_RS05515_lanthipeptideNZ_JAQCYD010000003.1 Lanthipeptides: PGX70_RS05515_lanthipeptide

Lanthipeptides: SMUGS5_RS08725_lanthipeptide

2

IFETVTYTVASWALGNNGHLCTVTVECQKNCQR

34

I TV + S LGN G++CTVTVEC +NC +

1

IGTTVVNSTFSIVLGNKGYICTVTVECMRNCSK

33

47.4%

NZ_CP013862.1and 1 other:

NZ_CP013862.1 Lanthipeptides: AOX59_RS09360_lanthipeptide

Lanthipeptides: AOX59_RS09360_lanthipeptide

6

VTYTVASW---ALGNNGHLCTVTVECQKNC

32

+TY+ W A+GN+GHLCT TVECQ NC

8

LTYSTCHWISPAMGNDGHLCTATVECQPNC

37

47.2%

NZ_CP086141.1and 1 other:

NZ_CP086141.1 Lanthipeptides: LL065_RS24800_lanthipeptide

Lanthipeptides: LL065_RS24800_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNC

32

TI + +AS+ALGN G +CT +VECQ NC

5

TILQGTVGCLASYALGNQGKICTWSVECQNNC

36

47.1%

NZ_CP047419.1and 3 others:

NZ_CP047419.1 Lanthipeptides: GTU71_RS12805_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptide

Lanthipeptides: GTU71_RS12805_lanthipeptide

5

TVTYTVASWALGNNGHLCTVTVECQKNCQ

33

T T+T+++ LGN G CT+TVECQ NC+

3

TNTFTLSN-VLGNQGGWCTLTVECQPNCK

30

47.1%

NZ_CP061033.1and 1 other:

NZ_CP061033.1 Lanthipeptides: IAU67_RS09320_lanthipeptide

Lanthipeptides: IAU67_RS09320_lanthipeptide

12

SWALGNNGHLCTVTVECQKNCQ

33

SWALGN G+ CT T+ECQK CQ

1

SWALGNEGNFCTGTIECQKTCQ

22

47.1%

NZ_WITJ01000001.1and 1 other:

NZ_WITJ01000001.1 Lanthipeptides: GHI93_RS00065_lanthipeptide

Lanthipeptides: GHI93_RS00065_lanthipeptide

3

FETVTYTVASWALGNNGHLCTVTVECQKNCQ

33

+ V +V + ALGNNG++CT T ECQ NC+

4

WNLVFSSVVTLALGNNGYVCTWTHECQANCK

34

45.0%

NZ_JALIRS010000003.1and 3 others:

NZ_JALIRS010000003.1 Lanthipeptides: MOK21_RS07255_lanthipeptideNZ_JALNLQ010000002.1 Lanthipeptides: MWJ95_RS06810_lanthipeptideNZ_JALNLQ010000002.1 Lanthipeptides: MWJ95_RS06810_lanthipeptide

Lanthipeptides: MOK21_RS07255_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNC

32

TI ++ AS+ LGN GH+CT+TVECQK C

8

TILTSLACAAASYILGNPGHVCTLTVECQKKC

39

44.7%

NZ_BMRY01000003.1and 24 others:

NZ_BMRY01000003.1 Lanthipeptides: IE140_RS12370_lanthipeptideNZ_BMSZ01000006.1 Lanthipeptides: IE135_RS12780_lanthipeptideNZ_BMSZ01000006.1 Lanthipeptides: IE135_RS12780_lanthipeptideNZ_BNBJ01000003.1 Lanthipeptides: IE304_RS05285_lanthipeptideNZ_BNBJ01000003.1 Lanthipeptides: IE304_RS05285_lanthipeptideNZ_CP021707.1 Lanthipeptides: CCZ36_RS18205_lanthipeptideNZ_CP079114.1 Lanthipeptides: KV381_RS19425_lanthipeptideNZ_CP079114.1 Lanthipeptides: KV381_RS19425_lanthipeptideNZ_CP084357.1 Lanthipeptides: LEL86_RS34965_lanthipeptideNZ_CP084357.1 Lanthipeptides: LEL86_RS34965_lanthipeptideNZ_JALDPQ010000054.1 Lanthipeptides: MQC87_RS11020_lanthipeptideNZ_JALDPQ010000054.1 Lanthipeptides: MQC87_RS11020_lanthipeptideNZ_JAMFLF010000021.1 Lanthipeptides: M1212_RS32880_lanthipeptideNZ_JAMFLF010000021.1 Lanthipeptides: M1212_RS32880_lanthipeptideNZ_JNLT01000014.1 Lanthipeptides: CD02_RS38630_lanthipeptideNZ_JNLT01000014.1 Lanthipeptides: CD02_RS38630_lanthipeptideNZ_JNZV01000231.1 Lanthipeptides: BG84_RS0100145_lanthipeptideNZ_JNZV01000231.1 Lanthipeptides: BG84_RS0100145_lanthipeptideNZ_KZ819178.1 Lanthipeptides: DBP20_RS18455_lanthipeptideNZ_KZ819178.1 Lanthipeptides: DBP20_RS18455_lanthipeptideNZ_LZRD01000017.1 Lanthipeptides: A3Q37_RS19720_lanthipeptideNZ_LZRD01000017.1 Lanthipeptides: A3Q37_RS19720_lanthipeptideNZ_NNBN01000001.1 Lanthipeptides: CG717_RS03735_lanthipeptideNZ_NNBN01000001.1 Lanthipeptides: CG717_RS03735_lanthipeptide

Lanthipeptides: IE140_RS12370_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQ

33

TI +++ AS+ LGNNG+ CT T ECQ NC+

6

TIVSSLSCYGASYVLGNNGNFCTATAECQNNCK

38

44.7%

NZ_WHOM01000078.1and 1 other:

NZ_WHOM01000078.1 Lanthipeptides: GCN81_RS29300_lanthipeptide

Lanthipeptides: GCN81_RS29300_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQ

33

T +++ AS+ LGNNG+ CT T ECQ NCQ

6

TTLSSLSCYGASYVLGNNGNFCTATAECQSNCQ

38

44.1%

NC_006322.1and 7 others:

NC_006322.1 Lanthipeptides: BLI_RS20295_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07805_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07805_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08835_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08835_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11865_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11865_lanthipeptide

Lanthipeptides: BLI_RS20295_lanthipeptide

7

TYTVASWALGNNGHLCTVTVECQKNCQ

33

T + S LGNNG+LCTVT EC +C

6

TCAILSKPLGNNGYLCTVTKECMPSCN

32

MIBiG 4.0 matches for IE257_RS28650

47.1%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

5

TVTYTVASWALGNNGHLCTVTVECQKNCQ

33

T T+++++ ALGNNG CT+T ECQ NC

3

TNTFSLSN-ALGNNGGWCTLTKECQPNCN

30

32.4%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

15

LGNNGHLCTVTVECQKNCQ

33

LGN G CT+TVEC +C

10

LGNKGAYCTLTVECMPSCN

28

17.9%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

22

CTVTVECQKNC

32

CT++ ECQ NC

28

CTLSKECQPNC

38

allorf_062610_062828

Leader:

MHRTWPVCTVVDGPLDQRVRSFCGVDHKGGLIAAALLFG

Core:

SVTLLADHQLATLGEIHVFHARRAQHRCSGGID

Core with dehydrations:

DhaVDhbLLADHQLADhbLGEIHVFHARRAQHRCysDhaGGID

antiSMASH-DB 4.0 matches for allorf_062610_062828

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: allorf_062610_062828_lanthipeptide

Lanthipeptides: allorf_062610_062828_lanthipeptide

1

SVTLLADHQLATLGEIHVFHARRAQHRCSGGID

33

SVTLLADHQLATLGEIHVFHARRAQHRCSGGID

1

SVTLLADHQLATLGEIHVFHARRAQHRCSGGID

33

24.2%

NZ_CP023699.1and 1 other:

NZ_CP023699.1 Lanthipeptides: CP970_RS41395_lanthipeptide

Lanthipeptides: CP970_RS41395_lanthipeptide

7

DHQLATLGEIHVFHARRAQHRCSG

30

D QL T+G + A +C+G

3

DSQLLTVGIVGTTQGCTAATKCTG

26

21.2%

NZ_CP022685.1and 1 other:

NZ_CP022685.1 Lanthipeptides: KY5_RS01355_lanthipeptide

Lanthipeptides: KY5_RS01355_lanthipeptide

7

DHQLATLGEIHVFHARRAQHRCSG

30

D Q+ T+G + A +C+G

3

DSQILTVGIVGTTQGCTAATKCTG

26

with 2 more sequence matches

21.2%

NZ_CP106798.1:

Lanthipeptides: allorf_8192812_8192934_lanthipeptide

20

HARRAQHRCSGGI

32

A R HRC G I

4

QAPRGTHRCPGMI

16

15.2%

NZ_FUXI01000002.1:

Lanthipeptides: B5D30_RS01240_lanthipeptide

6

ADHQLATLGEIHVFHARRAQHRC

28

D T+G + F + +++C

1

GDVNPQTVGSLWEFLTSKGEYKC

23

allorf_062892_063122

Leader:

MLSASFWLKNSLRPVRSSSRGSGTQGACRQTGEPQPAGCANAVTGA

Core:

GTARGNAGCTTVGASAGPIRSIRGSDTSRK

Core with dehydrations:

GDhbARGNAGCysDhbDhbVGADhaAGPIRDhaIRGDhaDDhbDhaRK

antiSMASH-DB 4.0 matches for allorf_062892_063122

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: allorf_062892_063122_lanthipeptide

Lanthipeptides: allorf_062892_063122_lanthipeptide

1

GTARGNAGCTTVGASAGPIRSIRGSDTSRK

30

GTARGNAGCTTVGASAGPIRSIRGSDTSRK

1

GTARGNAGCTTVGASAGPIRSIRGSDTSRK

30

29.4%

NZ_CP036170.1and 1 other:

NZ_JAQDJC010000039.1 Sactipeptides: allorf_5599_5736_sactipeptide

Sactipeptides: allorf_1765734_1765871_sactipeptide

2

TARGNAGCTTVGASAGPIRSIRGSD

26

T G AGCT ASA I + ++

7

TCVGCAGCTACWASATAISHVSAAN

31

26.7%

NZ_CP049945.1:

Lanthipeptides: allorf_6834749_6834883_lanthipeptide

5

GNAGCTTVGASAGPIRSIRGSDTSR

29

G +GC+ + P+R G SR

3

GRSGCSPLPPRFAPLRHRPGRQGSR

27

with 2 more sequence matches

23.3%

NZ_MQUS01000030.1:

Lanthipeptides: allorf_005405_005560_lanthipeptide

6

NAGCTTVGASAGPIR

20

N GCTT ++ P R

7

NCGCTTAPSTRWPQR

21

11.9%

NZ_CP041244.1:

Lanthipeptides: allorf_9037809_9038009_lanthipeptide

14

ASAGPIRSIRGSDTSR

29

A+AGP+R R ++ +R

44

ANAGPLRPFRAAEPAR

59

allorf_063400_063462

Leader:

MPGRSGCAGS

Core:

GPPRCASRGS

Core with dehydrations:

GPPRCysADhaRGDha

antiSMASH-DB 4.0 matches for allorf_063400_063462

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: allorf_063400_063462_lanthipeptide

Lanthipeptides: allorf_063400_063462_lanthipeptide

1

GPPRCASRGS

10

GPPRCASRGS

1

GPPRCASRGS

10

19.4%

NZ_BMUQ01000012.1:

Lanthipeptides: allorf_024174_024338_lanthipeptide

3

PRCASRG

9

PRC SRG

11

PRCTSRG

17

12.8%

NZ_AP026074.1:

Lanthipeptides: allorf_0737990_0738172_lanthipeptide

2

PPRCASRGS

10

PPRC + GS

25

PPRCTAVGS

33

with 1 more sequence matches

9.2%

NZ_CP012109.1and 1 other:

NZ_CP012109.1 Lanthipeptides: allorf_3526024_3526248_lanthipeptide

Lanthipeptides: allorf_3526024_3526248_lanthipeptide

1

GPPRCAS

7

GPPRC S

4

GPPRCVS

10

allorf_063434_063604

Leader:

MRARCSSDSWLMWAVGLHGS

Core:

GPRSSQRFLRIIFARSSCVVNREGGPGHARQERLCR

Core with dehydrations:

GPRDhaDhaQRFLRIIFARDhaDhaCysVVNREGGPGHARQERLCysR

antiSMASH-DB 4.0 matches for allorf_063434_063604

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: allorf_063434_063604_lanthipeptide

Lanthipeptides: allorf_063434_063604_lanthipeptide

1

GPRSSQRFLRIIFARSSCVVNREGGPGHARQERLCR

36

GPRSSQRFLRIIFARSSCVVNREGGPGHARQERLCR

1

GPRSSQRFLRIIFARSSCVVNREGGPGHARQERLCR

36

19.4%

NZ_CP101655.1and 1 other:

NZ_JAHHZH010000008.1 Lanthipeptides: allorf_106724_106882_lanthipeptide

Lanthipeptides: allorf_2707159_2707317_lanthipeptide

12

IFARSSCVVNREGGPGHARQERLC

35

++AR RE H R ++ C

1

VYARVFLEYKRETQNWHDRSDQWC

24

16.7%

NZ_FOGT01000008.1:

Lassopeptides: BMW59_RS21390_lassopeptide

24

GGPGHARQERLC

35

GGPG+A + C

1

GGPGNAIDDSYC

12

with 2 more sequence matches

16.7%

NZ_KN196470.1:

Lanthipeptides: allorf_0807110_0807271_lanthipeptide

25

GPGHARQERLC

35

G GHA Q C

3

GAGHASQASAC

13

12.7%

NZ_JANVAG010000003.1:

Lanthipeptides: allorf_428281_428505_lanthipeptide

1

GPRSSQRFLRIIFARSSCVVNREG

24

GPR ++ L + A C+++ G

23

GPRVAEAELELTSAEGPCILHAGG

46

MIBiG 4.0 matches for allorf_063434_063604

11.1%

BGC0000581.6 (AAD28494.1):

Lasso peptide: microcin J25

24

GGPGHARQ

31

GG GH +

1

GGAGHVPE

8

Lanthipeptide(s) for IE257_RS28645

IE257_RS28640

Leader:

MSNIVKDATTGFVSEEELVQLAEEPSELAAGAAASGILCGAGITTAITTFSAAMQGG

Core:

CPTSGCTGRC

Core with dehydrations:

CysPDhbDhaGCysDhbGRCys

antiSMASH-DB 4.0 matches for IE257_RS28640

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: IE257_RS28640_lanthipeptide

Lanthipeptides: IE257_RS28640_lanthipeptide

1

CPTSGCTGRC

10

CPTSGCTGRC

1

CPTSGCTGRC

10

72.7%

NZ_GG657754.1and 1 other:

NZ_GG657754.1 Lanthipeptides: SSOG_RS27835_lanthipeptide

Lanthipeptides: SSOG_RS27835_lanthipeptide

1

CPTSGCTGRC

10

CPTSGCT +C

1

CPTSGCTSKC

10

50.0%

NZ_CP061033.1and 1 other:

NZ_CP061033.1 Lanthipeptides: IAU67_RS09325_lanthipeptide

Lanthipeptides: IAU67_RS09325_lanthipeptide

1

CPTSGCTGRC

10

CPTSGCT C

7

CPTSGCTHSC

16

with 53 more sequence matches

Only the first 10 are displayed here.

50.0%

NZ_NTSF01000026.1and 1 other:

NZ_NTSF01000026.1 Lanthipeptides: CN325_RS31015_lanthipeptide

Lanthipeptides: CN325_RS31015_lanthipeptide

1

CPTSGCTGRC

10

CPTS CT RC

3

CPTSKCTTRC

12

46.2%

NZ_BJTV01000004.1and 5 others:

NZ_BJTV01000004.1 Lanthipeptides: TNCT1_RS35800_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptide

Lanthipeptides: TNCT1_RS35800_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT C

4

CPTTKCTSAC

13

35.3%

NZ_QKTG01000020.1and 1 other:

NZ_QKTG01000020.1 Lanthipeptides: DEI99_RS08060_lanthipeptide

Lanthipeptides: DEI99_RS08060_lanthipeptide

1

CPTSGCTGRC

10

CPT CT +C

8

CPTGACTSKC

17

33.3%

NZ_CP115450.1and 1 other:

NZ_CP115450.1 Lanthipeptides: O1G21_RS35405_lanthipeptide

Lanthipeptides: O1G21_RS35405_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT RC

12

CPTTACTSRC

21

26.1%

NZ_CP050134.2and 3 others:

NZ_EQ973330.1 Lanthipeptides: CORMATOL_RS14310_lanthipeptideNZ_LR134504.1 Lanthipeptides: EL293_RS13085_lanthipeptideNZ_LR134504.1 Lanthipeptides: EL293_RS13085_lanthipeptide

Lanthipeptides: HBA49_RS00775_lanthipeptide

1

CPTSGCTGRC

10

CPT+ C+ RC

14

CPTTRCSRRC

23

25.0%

NZ_FQZK01000006.1and 2 others:

NZ_FQZK01000006.1 Lanthipeptides: BUE45_RS13105_lanthipeptideNZ_FQZK01000006.1 Lanthipeptides: BUE45_RS13105_lanthipeptide

Lanthipeptides: BUE45_RS13105_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT RC

19

CPTTVCTSRC

28

25.0%

NZ_LR134483.1and 1 other:

NZ_LR134483.1 Lanthipeptides: EL241_RS13590_lanthipeptide

Lanthipeptides: EL241_RS13590_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT +C

14

CPTAKCTSKC

23

24.2%

NZ_NTSF01000026.1and 1 other:

NZ_NTSF01000026.1 Lanthipeptides: CN325_RS21840_lanthipeptide

Lanthipeptides: CN325_RS21840_lanthipeptide

1

CPTSGCTGRC

10

CPTS CT RC

20

CPTSKCTERC

29

22.6%

NZ_AP026714.1and 21 others:

NZ_AP026714.1 Lanthipeptides: PO853_RS06520_lanthipeptideNZ_CP028724.1 Lanthipeptides: C7I31_RS11270_lanthipeptideNZ_CP028724.1 Lanthipeptides: C7I31_RS11270_lanthipeptideNZ_CP092907.1 Lanthipeptides: MK744_RS03990_lanthipeptideNZ_CP092907.1 Lanthipeptides: MK744_RS03990_lanthipeptideNZ_KB944759.1 Lanthipeptides: WOA_RS09815_lanthipeptideNZ_KB944759.1 Lanthipeptides: WOA_RS09815_lanthipeptideNZ_KZ845892.1 Lanthipeptides: EB42_RS00090_lanthipeptideNZ_KZ845892.1 Lanthipeptides: EB42_RS00090_lanthipeptideNZ_KZ845958.1 Lanthipeptides: EB53_RS07695_lanthipeptideNZ_KZ845958.1 Lanthipeptides: EB53_RS07695_lanthipeptideNZ_LR607361.1 Lanthipeptides: FN738_RS03740_lanthipeptideNZ_LR607361.1 Lanthipeptides: FN738_RS03740_lanthipeptideNZ_LR607378.1 Lanthipeptides: FN751_RS00125_lanthipeptideNZ_LR607378.1 Lanthipeptides: FN751_RS00125_lanthipeptideNZ_LSFS01000007.1 Lanthipeptides: AW733_RS07745_lanthipeptideNZ_LSFS01000007.1 Lanthipeptides: AW733_RS07745_lanthipeptideNZ_NGMP01000002.1 Lanthipeptides: A5830_RS04680_lanthipeptideNZ_NGMP01000002.1 Lanthipeptides: A5830_RS04680_lanthipeptideNZ_NGMV01000001.1 Lanthipeptides: A5824_RS07985_lanthipeptideNZ_NGMV01000001.1 Lanthipeptides: A5824_RS07985_lanthipeptide

Lanthipeptides: PO853_RS06520_lanthipeptide

1

CPTSGCTGRC

10

CPTS CT C

22

CPTSVCTRAC

31

22.2%

NZ_KE384533.1and 2 others:

NZ_KE384533.1 Lanthipeptides: K329_RS31315_lanthipeptideNZ_KE384533.1 Lanthipeptides: K329_RS31315_lanthipeptide

Lanthipeptides: K329_RS31315_lanthipeptide

1

CPTSGCTGRC

10

CPT+ CT C

18

CPTTSCTDEC

27

MIBiG 4.0 matches for IE257_RS28640

23.1%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

1

CPTSGCTGRC

10

CPT+ CT +C

17

CPTTKCTSKC

26

18.9%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

1

CPTSGCTGRC

10

CPT+ CT RC

28

CPTTKCTSRC

37

IE257_RS28650

Leader:

MAAGGKRPRESKKRSSLMHNRNVDILEEINEQNLDGLSAG

Core:

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQR

Core with dehydrations:

DhbIFEDhbVDhbYDhbVADhaWALGNNGHLCysDhbVDhbVECysQKNCysQR

antiSMASH-DB 4.0 matches for IE257_RS28650

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: IE257_RS28650_lanthipeptide

Lanthipeptides: IE257_RS28650_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQR

34

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQR

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQR

34

64.9%

NZ_CP115450.1and 1 other:

NZ_CP115450.1 Lanthipeptides: O1G21_RS35415_lanthipeptide

Lanthipeptides: O1G21_RS35415_lanthipeptide

3

FETVTYTVASWALGNNGHLCTVTVECQKNCQR

34

E +TYT ASWA+GNNG+LCTVT ECQKNCQ

6

IEVLTYTAASWAMGNNGYLCTVTAECQKNCQH

37

52.9%

NZ_CP043405.1and 1 other:

NZ_CP043405.1 Lanthipeptides: FY406_RS04355_lanthipeptide

Lanthipeptides: FY406_RS04355_lanthipeptide

2

IFETVTYTVASWALGNNGHLCTVTVECQKNCQ

33

I TV + S LGN G++CTVTVEC +NCQ

1

IGTTVVNSTFSIVLGNKGYICTVTVECMRNCQ

32

with 98 more sequence matches

Only the first 10 are displayed here.

50.0%

NC_018089.1and 11 others:

NC_018089.1 Lanthipeptides: SMUGS5_RS08725_lanthipeptideNZ_CP044493.1 Lanthipeptides: FSA40_RS09065_lanthipeptideNZ_CP044493.1 Lanthipeptides: FSA40_RS09065_lanthipeptideNZ_CP050270.1 Lanthipeptides: HB753_RS00325_lanthipeptideNZ_CP050270.1 Lanthipeptides: HB753_RS00325_lanthipeptideNZ_JADPAN010000004.1 Lanthipeptides: I4U28_RS03985_lanthipeptideNZ_JADPAN010000004.1 Lanthipeptides: I4U28_RS03985_lanthipeptideNZ_JALDUN010000005.1 Lanthipeptides: M1F82_RS08120_lanthipeptideNZ_JALDUN010000005.1 Lanthipeptides: M1F82_RS08120_lanthipeptideNZ_JAQCYD010000003.1 Lanthipeptides: PGX70_RS05515_lanthipeptideNZ_JAQCYD010000003.1 Lanthipeptides: PGX70_RS05515_lanthipeptide

Lanthipeptides: SMUGS5_RS08725_lanthipeptide

2

IFETVTYTVASWALGNNGHLCTVTVECQKNCQR

34

I TV + S LGN G++CTVTVEC +NC +

1

IGTTVVNSTFSIVLGNKGYICTVTVECMRNCSK

33

47.4%

NZ_CP013862.1and 1 other:

NZ_CP013862.1 Lanthipeptides: AOX59_RS09360_lanthipeptide

Lanthipeptides: AOX59_RS09360_lanthipeptide

6

VTYTVASW---ALGNNGHLCTVTVECQKNC

32

+TY+ W A+GN+GHLCT TVECQ NC

8

LTYSTCHWISPAMGNDGHLCTATVECQPNC

37

47.2%

NZ_CP086141.1and 1 other:

NZ_CP086141.1 Lanthipeptides: LL065_RS24800_lanthipeptide

Lanthipeptides: LL065_RS24800_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNC

32

TI + +AS+ALGN G +CT +VECQ NC

5

TILQGTVGCLASYALGNQGKICTWSVECQNNC

36

47.1%

NZ_CP047419.1and 3 others:

NZ_CP047419.1 Lanthipeptides: GTU71_RS12805_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptide

Lanthipeptides: GTU71_RS12805_lanthipeptide

5

TVTYTVASWALGNNGHLCTVTVECQKNCQ

33

T T+T+++ LGN G CT+TVECQ NC+

3

TNTFTLSN-VLGNQGGWCTLTVECQPNCK

30

47.1%

NZ_CP061033.1and 1 other:

NZ_CP061033.1 Lanthipeptides: IAU67_RS09320_lanthipeptide

Lanthipeptides: IAU67_RS09320_lanthipeptide

12

SWALGNNGHLCTVTVECQKNCQ

33

SWALGN G+ CT T+ECQK CQ

1

SWALGNEGNFCTGTIECQKTCQ

22

47.1%

NZ_WITJ01000001.1and 1 other:

NZ_WITJ01000001.1 Lanthipeptides: GHI93_RS00065_lanthipeptide

Lanthipeptides: GHI93_RS00065_lanthipeptide

3

FETVTYTVASWALGNNGHLCTVTVECQKNCQ

33

+ V +V + ALGNNG++CT T ECQ NC+

4

WNLVFSSVVTLALGNNGYVCTWTHECQANCK

34

45.0%

NZ_JALIRS010000003.1and 3 others:

NZ_JALIRS010000003.1 Lanthipeptides: MOK21_RS07255_lanthipeptideNZ_JALNLQ010000002.1 Lanthipeptides: MWJ95_RS06810_lanthipeptideNZ_JALNLQ010000002.1 Lanthipeptides: MWJ95_RS06810_lanthipeptide

Lanthipeptides: MOK21_RS07255_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNC

32

TI ++ AS+ LGN GH+CT+TVECQK C

8

TILTSLACAAASYILGNPGHVCTLTVECQKKC

39

44.7%

NZ_BMRY01000003.1and 24 others:

NZ_BMRY01000003.1 Lanthipeptides: IE140_RS12370_lanthipeptideNZ_BMSZ01000006.1 Lanthipeptides: IE135_RS12780_lanthipeptideNZ_BMSZ01000006.1 Lanthipeptides: IE135_RS12780_lanthipeptideNZ_BNBJ01000003.1 Lanthipeptides: IE304_RS05285_lanthipeptideNZ_BNBJ01000003.1 Lanthipeptides: IE304_RS05285_lanthipeptideNZ_CP021707.1 Lanthipeptides: CCZ36_RS18205_lanthipeptideNZ_CP079114.1 Lanthipeptides: KV381_RS19425_lanthipeptideNZ_CP079114.1 Lanthipeptides: KV381_RS19425_lanthipeptideNZ_CP084357.1 Lanthipeptides: LEL86_RS34965_lanthipeptideNZ_CP084357.1 Lanthipeptides: LEL86_RS34965_lanthipeptideNZ_JALDPQ010000054.1 Lanthipeptides: MQC87_RS11020_lanthipeptideNZ_JALDPQ010000054.1 Lanthipeptides: MQC87_RS11020_lanthipeptideNZ_JAMFLF010000021.1 Lanthipeptides: M1212_RS32880_lanthipeptideNZ_JAMFLF010000021.1 Lanthipeptides: M1212_RS32880_lanthipeptideNZ_JNLT01000014.1 Lanthipeptides: CD02_RS38630_lanthipeptideNZ_JNLT01000014.1 Lanthipeptides: CD02_RS38630_lanthipeptideNZ_JNZV01000231.1 Lanthipeptides: BG84_RS0100145_lanthipeptideNZ_JNZV01000231.1 Lanthipeptides: BG84_RS0100145_lanthipeptideNZ_KZ819178.1 Lanthipeptides: DBP20_RS18455_lanthipeptideNZ_KZ819178.1 Lanthipeptides: DBP20_RS18455_lanthipeptideNZ_LZRD01000017.1 Lanthipeptides: A3Q37_RS19720_lanthipeptideNZ_LZRD01000017.1 Lanthipeptides: A3Q37_RS19720_lanthipeptideNZ_NNBN01000001.1 Lanthipeptides: CG717_RS03735_lanthipeptideNZ_NNBN01000001.1 Lanthipeptides: CG717_RS03735_lanthipeptide

Lanthipeptides: IE140_RS12370_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQ

33

TI +++ AS+ LGNNG+ CT T ECQ NC+

6

TIVSSLSCYGASYVLGNNGNFCTATAECQNNCK

38

44.7%

NZ_WHOM01000078.1and 1 other:

NZ_WHOM01000078.1 Lanthipeptides: GCN81_RS29300_lanthipeptide

Lanthipeptides: GCN81_RS29300_lanthipeptide

1

TIFETVTYTVASWALGNNGHLCTVTVECQKNCQ

33

T +++ AS+ LGNNG+ CT T ECQ NCQ

6

TTLSSLSCYGASYVLGNNGNFCTATAECQSNCQ

38

44.1%

NC_006322.1and 7 others:

NC_006322.1 Lanthipeptides: BLI_RS20295_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07805_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07805_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08835_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08835_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11865_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11865_lanthipeptide

Lanthipeptides: BLI_RS20295_lanthipeptide

7

TYTVASWALGNNGHLCTVTVECQKNCQ

33

T + S LGNNG+LCTVT EC +C

6

TCAILSKPLGNNGYLCTVTKECMPSCN

32

MIBiG 4.0 matches for IE257_RS28650

47.1%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

5

TVTYTVASWALGNNGHLCTVTVECQKNCQ

33

T T+++++ ALGNNG CT+T ECQ NC

3

TNTFSLSN-ALGNNGGWCTLTKECQPNCN

30

32.4%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

15

LGNNGHLCTVTVECQKNCQ

33

LGN G CT+TVEC +C

10

LGNKGAYCTLTVECMPSCN

28

17.9%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

22

CTVTVECQKNC

32

CT++ ECQ NC

28

CTLSKECQPNC

38

allorf_062610_062828

Leader:

MHRTWPVCTVVDGPLDQRVRSFCGVDHKGGLIAAALLFG

Core:

SVTLLADHQLATLGEIHVFHARRAQHRCSGGID

Core with dehydrations:

DhaVDhbLLADHQLADhbLGEIHVFHARRAQHRCysDhaGGID

antiSMASH-DB 4.0 matches for allorf_062610_062828

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: allorf_062610_062828_lanthipeptide

Lanthipeptides: allorf_062610_062828_lanthipeptide

1

SVTLLADHQLATLGEIHVFHARRAQHRCSGGID

33

SVTLLADHQLATLGEIHVFHARRAQHRCSGGID

1

SVTLLADHQLATLGEIHVFHARRAQHRCSGGID

33

24.2%

NZ_CP023699.1and 1 other:

NZ_CP023699.1 Lanthipeptides: CP970_RS41395_lanthipeptide

Lanthipeptides: CP970_RS41395_lanthipeptide

7

DHQLATLGEIHVFHARRAQHRCSG

30

D QL T+G + A +C+G

3

DSQLLTVGIVGTTQGCTAATKCTG

26

21.2%

NZ_CP022685.1and 1 other:

NZ_CP022685.1 Lanthipeptides: KY5_RS01355_lanthipeptide

Lanthipeptides: KY5_RS01355_lanthipeptide

7

DHQLATLGEIHVFHARRAQHRCSG

30

D Q+ T+G + A +C+G

3

DSQILTVGIVGTTQGCTAATKCTG

26

with 2 more sequence matches

21.2%

NZ_CP106798.1:

Lanthipeptides: allorf_8192812_8192934_lanthipeptide

20

HARRAQHRCSGGI

32

A R HRC G I

4

QAPRGTHRCPGMI

16

15.2%

NZ_FUXI01000002.1:

Lanthipeptides: B5D30_RS01240_lanthipeptide

6

ADHQLATLGEIHVFHARRAQHRC

28

D T+G + F + +++C

1

GDVNPQTVGSLWEFLTSKGEYKC

23

allorf_062892_063122

Leader:

MLSASFWLKNSLRPVRSSSRGSGTQGACRQTGEPQPAGCANAVTGA

Core:

GTARGNAGCTTVGASAGPIRSIRGSDTSRK

Core with dehydrations:

GDhbARGNAGCysDhbDhbVGADhaAGPIRDhaIRGDhaDDhbDhaRK

antiSMASH-DB 4.0 matches for allorf_062892_063122

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: allorf_062892_063122_lanthipeptide

Lanthipeptides: allorf_062892_063122_lanthipeptide

1

GTARGNAGCTTVGASAGPIRSIRGSDTSRK

30

GTARGNAGCTTVGASAGPIRSIRGSDTSRK

1

GTARGNAGCTTVGASAGPIRSIRGSDTSRK

30

29.4%

NZ_CP036170.1and 1 other:

NZ_JAQDJC010000039.1 Sactipeptides: allorf_5599_5736_sactipeptide

Sactipeptides: allorf_1765734_1765871_sactipeptide

2

TARGNAGCTTVGASAGPIRSIRGSD

26

T G AGCT ASA I + ++

7

TCVGCAGCTACWASATAISHVSAAN

31

26.7%

NZ_CP049945.1:

Lanthipeptides: allorf_6834749_6834883_lanthipeptide

5

GNAGCTTVGASAGPIRSIRGSDTSR

29

G +GC+ + P+R G SR

3

GRSGCSPLPPRFAPLRHRPGRQGSR

27

with 2 more sequence matches

23.3%

NZ_MQUS01000030.1:

Lanthipeptides: allorf_005405_005560_lanthipeptide

6

NAGCTTVGASAGPIR

20

N GCTT ++ P R

7

NCGCTTAPSTRWPQR

21

11.9%

NZ_CP041244.1:

Lanthipeptides: allorf_9037809_9038009_lanthipeptide

14

ASAGPIRSIRGSDTSR

29

A+AGP+R R ++ +R

44

ANAGPLRPFRAAEPAR

59

allorf_063400_063462

Leader:

MPGRSGCAGS

Core:

GPPRCASRGS

Core with dehydrations:

GPPRCysADhaRGDha

antiSMASH-DB 4.0 matches for allorf_063400_063462

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: allorf_063400_063462_lanthipeptide

Lanthipeptides: allorf_063400_063462_lanthipeptide

1

GPPRCASRGS

10

GPPRCASRGS

1

GPPRCASRGS

10

19.4%

NZ_BMUQ01000012.1:

Lanthipeptides: allorf_024174_024338_lanthipeptide

3

PRCASRG

9

PRC SRG

11

PRCTSRG

17

12.8%

NZ_AP026074.1:

Lanthipeptides: allorf_0737990_0738172_lanthipeptide

2

PPRCASRGS

10

PPRC + GS

25

PPRCTAVGS

33

with 1 more sequence matches

9.2%

NZ_CP012109.1and 1 other:

NZ_CP012109.1 Lanthipeptides: allorf_3526024_3526248_lanthipeptide

Lanthipeptides: allorf_3526024_3526248_lanthipeptide

1

GPPRCAS

7

GPPRC S

4

GPPRCVS

10

allorf_063434_063604

Leader:

MRARCSSDSWLMWAVGLHGS

Core:

GPRSSQRFLRIIFARSSCVVNREGGPGHARQERLCR

Core with dehydrations:

GPRDhaDhaQRFLRIIFARDhaDhaCysVVNREGGPGHARQERLCysR

antiSMASH-DB 4.0 matches for allorf_063434_063604

100.0%

NZ_BMVL01000013.1and 1 other:

NZ_BMVL01000013.1 Lanthipeptides: allorf_063434_063604_lanthipeptide

Lanthipeptides: allorf_063434_063604_lanthipeptide

1

GPRSSQRFLRIIFARSSCVVNREGGPGHARQERLCR

36

GPRSSQRFLRIIFARSSCVVNREGGPGHARQERLCR

1

GPRSSQRFLRIIFARSSCVVNREGGPGHARQERLCR

36

19.4%

NZ_CP101655.1and 1 other:

NZ_JAHHZH010000008.1 Lanthipeptides: allorf_106724_106882_lanthipeptide

Lanthipeptides: allorf_2707159_2707317_lanthipeptide

12

IFARSSCVVNREGGPGHARQERLC

35

++AR RE H R ++ C

1

VYARVFLEYKRETQNWHDRSDQWC

24

16.7%

NZ_FOGT01000008.1:

Lassopeptides: BMW59_RS21390_lassopeptide

24

GGPGHARQERLC

35

GGPG+A + C

1

GGPGNAIDDSYC

12

with 2 more sequence matches

16.7%

NZ_KN196470.1:

Lanthipeptides: allorf_0807110_0807271_lanthipeptide

25

GPGHARQERLC

35

G GHA Q C

3

GAGHASQASAC

13

12.7%

NZ_JANVAG010000003.1:

Lanthipeptides: allorf_428281_428505_lanthipeptide

1

GPRSSQRFLRIIFARSSCVVNREG

24

GPR ++ L + A C+++ G

23

GPRVAEAELELTSAEGPCILHAGG

46

MIBiG 4.0 matches for allorf_063434_063604

11.1%

BGC0000581.6 (AAD28494.1):

Lasso peptide: microcin J25

24

GGPGHARQ

31

GG GH +

1

GGAGHVPE

8

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

Additional weak hits (4):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.87 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.27 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.84 out of 26.06

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

IE257_RS28640 - Class II

Cleavage pHMM score: -2.50

RODEO score: 0

Monoisotopic mass: 929.4 Da

Molecular weight: 930.1 Da

Number of bridges: 3

IE257_RS28650 - Class II

Cleavage pHMM score: -4.90

RODEO score: 0

Monoisotopic mass: 3659.8 Da

Molecular weight: 3662.1 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3680.1 Da N = 1; 3698.1 Da N = 2; 3716.2 Da N = 3; 3734.2 Da N = 4

allorf_062610_062828 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 3535.9 Da

Molecular weight: 3537.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3556.0 Da N = 1; 3574.0 Da N = 2; 3592.0 Da N = 3

allorf_062892_063122 - Class II

Cleavage pHMM score: -4.60

RODEO score: 0

Monoisotopic mass: 2759.5 Da

Molecular weight: 2761.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2779.0 Da N = 1; 2797.0 Da N = 2; 2815.1 Da N = 3; 2833.1 Da N = 4; 2851.1 Da N = 5; 2869.1 Da N = 6; 2887.1 Da N = 7

allorf_063400_063462 - Class II

Cleavage pHMM score: -6.20

RODEO score: 0

Monoisotopic mass: 950.5 Da

Molecular weight: 951.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 969.1 Da N = 1

allorf_063434_063604 - Class II

Cleavage pHMM score: -6.60

RODEO score: 0

Monoisotopic mass: 4023.1 Da

Molecular weight: 4025.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 4043.6 Da N = 1; 4061.6 Da N = 2

IE257_RS28640 - Class II

Cleavage pHMM score: -2.50

RODEO score: 0

Monoisotopic mass: 929.4 Da

Molecular weight: 930.1 Da

Number of bridges: 3

IE257_RS28650 - Class II

Cleavage pHMM score: -4.90

RODEO score: 0

Monoisotopic mass: 3659.8 Da

Molecular weight: 3662.1 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3680.1 Da N = 1; 3698.1 Da N = 2; 3716.2 Da N = 3; 3734.2 Da N = 4

allorf_062610_062828 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 3535.9 Da

Molecular weight: 3537.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3556.0 Da N = 1; 3574.0 Da N = 2; 3592.0 Da N = 3

allorf_062892_063122 - Class II

Cleavage pHMM score: -4.60

RODEO score: 0

Monoisotopic mass: 2759.5 Da

Molecular weight: 2761.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2779.0 Da N = 1; 2797.0 Da N = 2; 2815.1 Da N = 3; 2833.1 Da N = 4; 2851.1 Da N = 5; 2869.1 Da N = 6; 2887.1 Da N = 7

allorf_063400_063462 - Class II

Cleavage pHMM score: -6.20

RODEO score: 0

Monoisotopic mass: 950.5 Da

Molecular weight: 951.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 969.1 Da N = 1

allorf_063434_063604 - Class II

Cleavage pHMM score: -6.60

RODEO score: 0

Monoisotopic mass: 4023.1 Da

Molecular weight: 4025.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 4043.6 Da N = 1; 4061.6 Da N = 2

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000013.1 - Region 3 - NI-siderophore

Download region GenBank file

Download region SVG

Location: 145,617 - 178,247 nt. (total: 32,631 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

IE257_RS29015: Antibiotic biosynthesis monooxygenase

smcogs: SMCOG1258: Antibiotic biosynthesis monooxygenase

Transferases: aminotransferase

IE257_RS29065: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Isomerases: mutase

IE257_RS29010: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.55	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0000942.4	0.54	other (other)	petrobactin	Bacillus anthracis str. Ames
BGC0001713.5	0.52	other (other)	ochrobactin	Ochrobactrum anthropi
BGC0002683.2	0.51	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0000295.5	0.51	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0002633.2	0.51	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0002470.2	0.50	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0000939.5	0.50	other (other)	aerobactin	Grimontia hollisae
BGC0001572.3	0.50	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0000852.5	0.49	other (other)	ectoine	Sporosarcina pasteurii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.55	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0000942.4	0.54	other (other)	petrobactin	Bacillus anthracis str. Ames
BGC0001713.5	0.52	other (other)	ochrobactin	Ochrobactrum anthropi
BGC0002683.2	0.51	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0000295.5	0.51	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0002633.2	0.51	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0002470.2	0.50	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0000939.5	0.50	other (other)	aerobactin	Grimontia hollisae
BGC0001572.3	0.50	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0000852.5	0.49	other (other)	ectoine	Sporosarcina pasteurii

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.35 out of 20.47

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.54 out of 18.46

Confidence: medium

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 24.62 out of 27.18

Confidence: strong

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.93 out of 27.18

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.93 out of 24.78

Confidence: medium

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 21.68 out of 23.43

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

Additional weak hits (11):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 25.31

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.31 out of 29.38

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.56 out of 24.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.59 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.92 out of 26.06

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.09 out of 29.65

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.77 out of 25.88

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.95 out of 23.43

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.11 out of 23.43

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.13 out of 35.63

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
BldD	Development and antibiotic global regulator
CRP	Cyclic AMP receptor protein
LexA variant 2	Repressor of DNA damage response
NrdR	Represses ribonucleotide reductase encoding genes
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000014.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 24,294 nt. (total: 24,294 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS29350: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

IE257_RS29430: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

Ligases: synthase

IE257_RS29340: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.57	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001273.3	0.57	PKS	asperlactone	Aspergillus ochraceus
BGC0001164.3	0.57	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.56	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002171.2	0.47	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.47	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0000013.3	0.43	PKS	alternariol	Aspergillus nidulans FGSC A4
BGC0000528.5	0.43	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001400.3	0.42	PKS	citreoviridin	Aspergillus terreus NIH2624

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.57	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001273.3	0.57	PKS	asperlactone	Aspergillus ochraceus
BGC0001164.3	0.57	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.56	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002171.2	0.47	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.47	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0000013.3	0.43	PKS	alternariol	Aspergillus nidulans FGSC A4
BGC0000528.5	0.43	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001400.3	0.42	PKS	citreoviridin	Aspergillus terreus NIH2624

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.88 out of 20.47

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.60 out of 19.26

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 22.41 out of 27.04

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.86 out of 27.04

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.68 out of 19.52

Confidence: medium

Additional weak hits (5):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.26 out of 36.54

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.03 out of 25.88

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.09 out of 23.43

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.95 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.16 out of 20.97

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

IE257_RS29340: mal

PKS_AT (577..878): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.5
inactive: 88.1
Methoxymalonyl-CoA: 52.5
Methylmalonyl-CoA: 34.9
Propionyl-CoA: 23.1
Acetyl-CoA: 19.8
Isobutyryl-CoA: 19.1
Benzoyl-CoA: 7.1
3-Methylbutyryl-CoA: 3.7
2-Methylbutyryl-CoA: 3.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Ethylmalonyl-CoA: -2.2

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
DasR	N-acetylglucosamine dependent repressor
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000018.1 - Region 1 - butyrolactone

Download region GenBank file

Download region SVG

Location: 1 - 8,064 nt. (total: 8,064 nt) Show pHMM detection rules used

Region on contig edge.

butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

IE257_RS32910: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Lyases: dehydratase

IE257_RS32885: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000848.5	0.77	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000850.5	0.62	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0000849.4	0.58	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0002578.2	0.40	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0001364.3	0.36	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0002535.2	0.35	NRPS	γ-poly-L-2,4-diaminobutyric acid	Streptomyces celluloflavus
BGC0001223.5	0.32	other (other)	hydroxysporine	uncultured bacterium
BGC0000253.4	0.32	PKS	oviedomycin	Streptomyces antibioticus
BGC0000665.5	0.31	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000813.3	0.31	other (other)	K-252a	Nonomuraea longicatena

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000848.5	0.77	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000850.5	0.62	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0000849.4	0.58	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0002578.2	0.40	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0001364.3	0.36	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0002535.2	0.35	NRPS	γ-poly-L-2,4-diaminobutyric acid	Streptomyces celluloflavus
BGC0001223.5	0.32	other (other)	hydroxysporine	uncultured bacterium
BGC0000253.4	0.32	PKS	oviedomycin	Streptomyces antibioticus
BGC0000665.5	0.31	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000813.3	0.31	other (other)	K-252a	Nonomuraea longicatena

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.94 out of 23.79

Confidence: medium

Additional weak hits (4):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.51 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.43 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.55 out of 18.57

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.75 out of 23.43

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_BMVL01000018.1 - Region 2 - NRPS

Download region GenBank file

Download region SVG

Location: 41,326 - 86,062 nt. (total: 44,737 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

IE257_RS33125: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

IE257_RS33140: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IE257_RS33195: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: PPTase acyltransferase methyltransferase

IE257_RS33080: methyltransferase

smcogs: SMCOG1248: methyltransferase

IE257_RS33120: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

IE257_RS33235: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: deformylase

IE257_RS33205: peptide deformylase

smcogs: SMCOG1275: peptide deformylase

Isomerases: isomerase

IE257_RS33050: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

IE257_RS33075: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

IE257_RS33130: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS33135: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001166.5	0.66	NRPS	HC-toxin	Alternaria jesenskae
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001251.4	0.66	NRPS	epichloenin A	Epichloe festucae
BGC0001250.4	0.66	NRPS	epichloenin A	Epichloe festucae
BGC0000357.5	0.65	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001166.5	0.66	NRPS	HC-toxin	Alternaria jesenskae
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001251.4	0.66	NRPS	epichloenin A	Epichloe festucae
BGC0001250.4	0.66	NRPS	epichloenin A	Epichloe festucae
BGC0000357.5	0.65	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.13 out of 23.79

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.44 out of 26.85

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.43 out of 23.79

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.93 out of 30.25

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.72 out of 27.04

Confidence: medium

Additional weak hits (5):

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.05 out of 19.26

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.86 out of 21.55

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.71 out of 20.01

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.90 out of 24.78

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.32 out of 26.85

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 41325 - 86062:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS33130: X

AMP-binding (28..462): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NVCLHDMGLISSVAGDGAETPMYGLAEATLAVAM
Nearest Stachelhaus code(s):: Pca DMGVLGCLMK (62% 8Å match); Trp DMIICGCAAK (38% 8Å match); niCpa DPIVVGGVAK (29% 8Å match)
Stachelhaus code match:: 60% (weak)

IE257_RS33135: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (553..963): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDISFDLSVYDGNTLLSGDISLGGATEAAIWSIW
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ColR	Phenol-responsive regulator
DasR	N-acetylglucosamine dependent repressor
IolR	Regulator of the inositol catabolism
RutR	Regulator of the pyrimidine and purine metabolism

NZ_BMVL01000021.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 82,340 - 107,518 nt. (total: 25,179 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

IE257_RS39565: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: deaminase hydrolase

IE257_RS35480: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

IE257_RS35500: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

Ligases: ligase synthase

IE257_RS35490: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IE257_RS35495: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

IE257_RS35530: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.67	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.67	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 20.58 out of 23.05

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (10):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 21.91 out of 31.73

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.73 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.50 out of 27.78

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 20.94 out of 33.14

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.71 out of 36.53

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.94 out of 33.73

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.78 out of 32.74

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.44 out of 29.38

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 82339 - 107518:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS35490: X

AMP-binding (51..456): X

nrpys: Dpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HAKAFDISIWQCELLATGEVNAYGPTECSDDVTH
Nearest Stachelhaus code(s):: Dpr DIWELTADDK (94% 8Å match); Dab DIWELTADDK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS35530: X - Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (7..217): X

nrpys: Asp, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ---A--------KAVLGGEFNHYGPTETTIGVAT
Nearest Stachelhaus code(s):: Asp DLTKVGHIGK (65% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (894..1290): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: HAATFDIAALELQALVGGEVNLYGPTEATVWATS
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
VqsM	Regulator of quorum-sensing signalling systems

NZ_BMVL01000022.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 71,477 nt. (total: 71,477 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

IE257_RS35555: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

IE257_RS35560: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: PPTase

IE257_RS35585: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

Hydrolases: esterase

IE257_RS35575: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

IE257_RS35540: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

IE257_RS35545: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

IE257_RS39050: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0000067.5	0.64	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0000067.5	0.64	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.06 out of 28.19

Confidence: medium

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 22.11 out of 27.18

Confidence: medium

Additional weak hits (5):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.79 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.91 out of 31.73

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.54 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.28 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 71477:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ohmal - Me-ohmal - redmal) + (ohmal - Me-ohmal - ccmal) + (pk - ccmal) + (pk)
Non-colinear ordering:: IE257_RS39050 + IE257_RS35545 + IE257_RS35540 + IE257_RS35535

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS35535: pk - pk

PKS_AT (559..856): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 214.5
Methylmalonyl-CoA: 208.1
Methoxymalonyl-CoA: 173.5
2-Methylbutyryl-CoA: 139.5
trans-1,2-CPDA: 122.1
Propionyl-CoA: 112.8
Malonyl-CoA: 104.2
CHC-CoA: 95.8
Isobutyryl-CoA: 94.4
Benzoyl-CoA: 72.3
Acetyl-CoA: 53.5
fatty_acid: 47.3
inactive: 44.8
3-Methylbutyryl-CoA: 42.0

PKS_AT (2077..2291): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Methoxymalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 79.2%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 154.2
Methylmalonyl-CoA: 153.8
Methoxymalonyl-CoA: 139.8
2-Methylbutyryl-CoA: 92.8
trans-1,2-CPDA: 88.1
Propionyl-CoA: 79.6
Malonyl-CoA: 77.9
CHC-CoA: 68.8
Isobutyryl-CoA: 55.8
Benzoyl-CoA: 54.0
fatty_acid: 36.6
Acetyl-CoA: 29.2
inactive: 27.4
3-Methylbutyryl-CoA: 20.7

IE257_RS35540: pk - mal

PKS_AT (569..867): pk

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 79.2%; Methylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.3
Ethylmalonyl-CoA: 168.5
Methoxymalonyl-CoA: 146.7
2-Methylbutyryl-CoA: 121.6
trans-1,2-CPDA: 98.1
Propionyl-CoA: 92.6
Isobutyryl-CoA: 90.7
Malonyl-CoA: 78.7
CHC-CoA: 77.0
fatty_acid: 49.5
Benzoyl-CoA: 47.4
3-Methylbutyryl-CoA: 41.2
Acetyl-CoA: 40.3
inactive: 27.4

PKS_AT (2644..2943): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 195.6
inactive: 103.0
Methylmalonyl-CoA: 53.0
Methoxymalonyl-CoA: 49.7
Isobutyryl-CoA: 27.8
Propionyl-CoA: 26.9
Benzoyl-CoA: 26.5
Ethylmalonyl-CoA: 23.8
Acetyl-CoA: 20.9
CHC-CoA: 16.7
2-Methylbutyryl-CoA: 10.9
trans-1,2-CPDA: 9.7
fatty_acid: 6.8
3-Methylbutyryl-CoA: 0.0

IE257_RS35545: mal - mmal - mal

PKS_AT (561..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.6
inactive: 60.9
Methoxymalonyl-CoA: 59.9
Methylmalonyl-CoA: 44.1
Propionyl-CoA: 34.3
Ethylmalonyl-CoA: 26.7
Benzoyl-CoA: 25.8
Isobutyryl-CoA: 22.8
Acetyl-CoA: 15.3
CHC-CoA: 15.2
2-Methylbutyryl-CoA: 14.8
trans-1,2-CPDA: 9.2
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1996..2290): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.5
Ethylmalonyl-CoA: 203.8
Methoxymalonyl-CoA: 172.4
2-Methylbutyryl-CoA: 146.3
trans-1,2-CPDA: 135.4
Propionyl-CoA: 117.1
CHC-CoA: 95.1
Malonyl-CoA: 87.5
Isobutyryl-CoA: 80.7
Benzoyl-CoA: 68.6
Acetyl-CoA: 55.3
3-Methylbutyryl-CoA: 36.3
fatty_acid: 32.7
inactive: 23.9

PKS_AT (3522..3815): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.1
inactive: 106.2
Methoxymalonyl-CoA: 67.4
Methylmalonyl-CoA: 60.0
Propionyl-CoA: 44.4
2-Methylbutyryl-CoA: 32.8
Isobutyryl-CoA: 31.3
Acetyl-CoA: 20.8
Ethylmalonyl-CoA: 19.7
CHC-CoA: 15.8
Benzoyl-CoA: 13.9
fatty_acid: 13.5
trans-1,2-CPDA: 9.0
3-Methylbutyryl-CoA: 0.0

IE257_RS39050: mal - mal - mmal - mal

PKS_AT (512..808): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.9
inactive: 112.0
Methoxymalonyl-CoA: 60.7
Methylmalonyl-CoA: 47.8
Propionyl-CoA: 32.0
2-Methylbutyryl-CoA: 22.5
Ethylmalonyl-CoA: 20.0
Isobutyryl-CoA: 15.2
trans-1,2-CPDA: 15.1
CHC-CoA: 14.9
Benzoyl-CoA: 14.9
Acetyl-CoA: 14.6
fatty_acid: 11.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (1508..1799): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.7
inactive: 100.1
Methylmalonyl-CoA: 63.1
Methoxymalonyl-CoA: 57.9
Propionyl-CoA: 43.1
Isobutyryl-CoA: 27.2
fatty_acid: 23.7
Ethylmalonyl-CoA: 19.9
Acetyl-CoA: 19.0
Benzoyl-CoA: 17.1
CHC-CoA: 14.9
trans-1,2-CPDA: 14.6
2-Methylbutyryl-CoA: 12.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (2982..3280): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.2
Ethylmalonyl-CoA: 178.6
Methoxymalonyl-CoA: 156.3
2-Methylbutyryl-CoA: 115.9
CHC-CoA: 105.2
Propionyl-CoA: 100.2
trans-1,2-CPDA: 96.6
Malonyl-CoA: 86.4
Isobutyryl-CoA: 76.3
Benzoyl-CoA: 57.7
Acetyl-CoA: 51.6
3-Methylbutyryl-CoA: 28.2
fatty_acid: 26.9
inactive: 17.1

PKS_AT (4497..4787): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.3
inactive: 99.6
Methoxymalonyl-CoA: 61.2
Methylmalonyl-CoA: 49.1
Propionyl-CoA: 37.3
fatty_acid: 29.0
Isobutyryl-CoA: 23.9
Acetyl-CoA: 20.8
Ethylmalonyl-CoA: 19.9
Benzoyl-CoA: 19.0
2-Methylbutyryl-CoA: 16.4
CHC-CoA: 12.6
trans-1,2-CPDA: 9.4
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
NrdR	Represses ribonucleotide reductase encoding genes
ZuR variant 1	Zinc-responsive repressor

NZ_BMVL01000023.1 - Region 1 - azole-containing-RiPP

Download region GenBank file

Download region SVG

Location: 39,624 - 69,385 nt. (total: 29,762 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

IE257_RS35985: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: dehydratase

IE257_RS35960: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000606.5	0.65	ribosomal	lactazole	Streptomyces lactacystinaeus
BGC0001640.3	0.50	ribosomal	kocurin	Kocuria rosea
BGC0001174.4	0.47	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0001146.5	0.46	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0001145.5	0.45	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000605.5	0.45	ribosomal	GE37468	Streptomyces sp. ATCC 55365
BGC0001173.5	0.43	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000569.5	0.43	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0000613.5	0.43	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001860.4	0.43	ribosomal	thiomuracin	Thermobispora bispora DSM 43833

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000606.5	0.65	ribosomal	lactazole	Streptomyces lactacystinaeus
BGC0001640.3	0.50	ribosomal	kocurin	Kocuria rosea
BGC0001174.4	0.47	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0001146.5	0.46	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0001145.5	0.45	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000605.5	0.45	ribosomal	GE37468	Streptomyces sp. ATCC 55365
BGC0001173.5	0.43	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000569.5	0.43	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0000613.5	0.43	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001860.4	0.43	ribosomal	thiomuracin	Thermobispora bispora DSM 43833

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.38 out of 29.72

Confidence: medium

Additional weak hits (10):

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.75 out of 23.43

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.82 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.61 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.04 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.88 out of 28.14

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.14 out of 36.54

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.41 out of 20.97

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.48 out of 29.65

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.99 out of 26.06

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.67 out of 26.85

Confidence: weak

(Thi)azol(in)e containing peptide predictions

(Thi)azol(in)e containing peptide(s)

IE257_RS35965

Leader:

MNDGTPSSGFDLDDLDLGELTVTSMRDTVALPEGGA

Core:

SNGGSSCSCGSSSCAHPQLPELPV

Core with dehydrations:

DhaNGGDhaDhaCysDhaCysGDhaDhaDhaCysAHPQLPELPV

antiSMASH-DB 4.0 matches for IE257_RS35965

100.0%

NZ_BMVL01000023.1:

Thiopeptides: IE257_RS35965_thiopeptide

1

SNGGSSCSCGSSSCAHPQLPELPV

24

SNGGSSCSCGSSSCAHPQLPELPV

1

SNGGSSCSCGSSSCAHPQLPELPV

24

95.8%

NZ_CP020039.1and 1 other:

NZ_CP020555.1 Thiopeptides: B6R96_RS00365_thiopeptide

Thiopeptides: B4U39_RS00310_thiopeptide

1

SNGGSSCSCGSSSCAHPQLPELPV

24

SNGGSSCSCGSSSCAHPQLP+LPV

1

SNGGSSCSCGSSSCAHPQLPDLPV

24

95.8%

NZ_CP071139.1:

Thiopeptides: JYK04_RS00935_thiopeptide

1

SNGGSSCSCGSSSCAHPQLPELPV

24

SNGGSSCSCGSSSCAHPQLPELP+

1

SNGGSSCSCGSSSCAHPQLPELPL

24

with 10 more sequence matches

88.0%

NZ_LIVQ01000005.1:

Thiopeptides: AMK16_RS20635_thiopeptide

1

SNGGSSCSCGSSSCAHPQLPELP

23

SNGGSSCSCGSSSCAHPQLP+LP

1

SNGGSSCSCGSSSCAHPQLPDLP

23

70.8%

NZ_CP009438.1and 1 other:

NZ_LIVZ01000003.1 Thiopeptides: AMK31_RS07340_thiopeptide

Thiopeptides: SGLAU_RS00695_thiopeptide

1

SNGGSSCSCGSSSCAHPQLP

20

SNG SSCSC SSSCA PQLP

1

SNGASSCSCSSSSCAQPQLP

20

50.0%

NZ_CP023701.1:

Thiopeptides: CP968_RS31560_thiopeptide

6

SCSC-GSSSCAHPQLPELPV

24

+CSC GSSSCA P +PV

4

TCSCQGSSSCAQPDQTGVPV

23

48.0%

NZ_CP023701.1:

Thiopeptides: CP968_RS31565_thiopeptide

6

SCSC-GSSSCAHPQ

18

+CSC GSSSCAHPQ

4

TCSCQGSSSCAHPQ

17

41.7%

NZ_CP064192.1:

Thiopeptides: IOD16_RS19015_thiopeptide

8

SCGSSSCAHPQLPE

21

SCGSS C PQLP+

1

SCGSSCCCQPQLPQ

14

28.1%

NC_021177.1and 1 other:

NZ_BMUG01000001.1 Lanthipeptides: allorf_0374269_0374400_lanthipeptide

Lanthipeptides: allorf_2593479_2593610_lanthipeptide

5

SSCSCGSSSCAHPQLPEL

22

+S SC S HP+LPE+

6

TSLSCPSRHPHHPRLPEI

23

25.0%

NZ_LIPD01000006.1:

Thiopeptides: AMK13_RS18030_thiopeptide

15

AHPQLPELP

23

A PQLP++P

14

AQPQLPDVP

22

22.2%

NZ_KB235904.1:

Lanthipeptides: OSCIL6407_RS0121590_lanthipeptide

7

CSCGSSSCAHP

17

C+CGS+ C+ P

1

CTCGSTQCSMP

11

21.1%

NZ_SNZH01000011.1:

Lanthipeptides: DFR29_RS18040_lanthipeptide

3

GGSSCSCGSSSCA

15

GG++ SC SSCA

24

GGTTASCRPSSCA

36

13.5%

NZ_CP059572.1:

Lanthipeptides: AGRA3207_RS24970_lanthipeptide

5

SSCSCGSSSCAH

16

+SC C SS C+H

30

NSCQCASSGCSH

41

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Types:

Type I: Featuring genes encoding indolic acid side ring (series e)
Type II: Featuring the gene coding for quinaldic acid moiety formation (series a, b, and c)
Type III: No genes for synthesizing L-Trp derivatives (series d)

RREFinder

TFBS Finder

Azole RiPPs

RRE predictions

: RRE type: Thiopeptide_F_RRE; Location: 28 - 107; Score: 52.4

TFBS definitions

Regulator	Description
NtrC	Nitrogen regulatory protein C
VqsM	Regulator of quorum-sensing signalling systems

Core peptide predictions

IE257_RS35965

Putative Type III

Cleavage pHMM score: 9.30

RODEO score: 23

Monoisotopic mass: 2303.0 Da

Molecular weight: 2304.5 Da

Predicted peptide features:
Central ring: pyridine trisubstituted

Alternative weights: (assuming N unmodified Ser/Thr residues); 2322.5 Da N = 1; 2340.5 Da N = 2; 2358.6 Da N = 3; 2376.6 Da N = 4; 2394.6 Da N = 5; 2412.6 Da N = 6; 2430.6 Da N = 7

NZ_BMVL01000028.1 - Region 1 - butyrolactone

Download region GenBank file

Download region SVG

Location: 18,005 - 26,839 nt. (total: 8,835 nt) Show pHMM detection rules used

Region on contig edge.

butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

IE257_RS37070: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Lyases: dehydratase

IE257_RS37080: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000848.5	0.77	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000849.4	0.71	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000850.5	0.65	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0002578.2	0.46	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0001224.5	0.42	other (other)	reductasporine	uncultured bacterium
BGC0000885.3	0.36	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0001513.3	0.35	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0002007.2	0.35	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0001374.5	0.35	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001458.4	0.35	other (aminocoumarin)	salinipostin G, Salinipostin G, Salinipostin A, Salinipostin B, Salinipostin C, Salinipostin D, Salinipostin E, Salinipostin F, Salinipostin H, Salinipostin I, Salinipostin J, Salinipostin K	Salinispora tropica CNB-440

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000848.5	0.77	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000849.4	0.71	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000850.5	0.65	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0002578.2	0.46	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0001224.5	0.42	other (other)	reductasporine	uncultured bacterium
BGC0000885.3	0.36	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0001513.3	0.35	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0002007.2	0.35	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0001374.5	0.35	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001458.4	0.35	other (aminocoumarin)	salinipostin G, Salinipostin G, Salinipostin A, Salinipostin B, Salinipostin C, Salinipostin D, Salinipostin E, Salinipostin F, Salinipostin H, Salinipostin I, Salinipostin J, Salinipostin K	Salinispora tropica CNB-440

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.85 out of 30.12

Confidence: medium

Additional weak hits (5):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.18 out of 29.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.25 out of 29.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.09 out of 35.63

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.07 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
OsdR	Development and stress management regulator

NZ_BMVL01000031.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 19,625 nt. (total: 19,625 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37260: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS37285: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.74	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.74	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 19625:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Asn) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS37260: X

AMP-binding (483..878): X

nrpys: Hpg, Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LVFAFDASVLDVSLVVGSEVHAYGPTEAAVITAV
Nearest Stachelhaus code(s):: Hpg DALSIGAVIK (71% 8Å match); Dhpg DALSVGTVIK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)

IE257_RS37285: X - X - Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (803..1194): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TALAFDNFCLELTLEVGGEFNTYGPTETTVDALS
Nearest Stachelhaus code(s):: Lys DNESGGTVAK (56% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1853..2243): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LGHAFDTFGWEMTLVTAGEFNSYGPTETTVDATL
Nearest Stachelhaus code(s):: His DTWTIASVDK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (2905..3305): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTVTSVKVVVGGEVNEYGPTETVVGCAV
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

NZ_BMVL01000032.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 18,572 nt. (total: 18,572 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

IE257_RS37290: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IE257_RS37295: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

IE257_RS37305: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Ligases: synthase

IE257_RS37300: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001164.3	0.60	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001268.4	0.58	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000041.3	0.57	PKS	oronofacic acid	Pseudomonas syringae
BGC0002631.2	0.54	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0000143.5	0.53	PKS	salinomycin	Streptomyces albus
BGC0000117.5	0.49	PKS	oligomycin	Streptomyces avermitilis
BGC0001544.3	0.49	NRPS, PKS	chlorflavonin	Aspergillus campestris IBT 28561

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001164.3	0.60	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001268.4	0.58	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000041.3	0.57	PKS	oronofacic acid	Pseudomonas syringae
BGC0002631.2	0.54	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0000143.5	0.53	PKS	salinomycin	Streptomyces albus
BGC0000117.5	0.49	PKS	oligomycin	Streptomyces avermitilis
BGC0001544.3	0.49	NRPS, PKS	chlorflavonin	Aspergillus campestris IBT 28561

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.67 out of 30.25

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 18572:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccemal) + (pk - ccmal)
Non-colinear ordering:: IE257_RS37290 + IE257_RS37300

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS37290: emal

PKS_AT (0..205): emal

ATSignature: (unknown)

No matches above 50%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 112.3
Methylmalonyl-CoA: 110.6
Methoxymalonyl-CoA: 91.1
trans-1,2-CPDA: 63.2
Propionyl-CoA: 59.3
2-Methylbutyryl-CoA: 58.4
Malonyl-CoA: 47.3
Isobutyryl-CoA: 41.9
Benzoyl-CoA: 32.9
3-Methylbutyryl-CoA: 28.4
fatty_acid: 27.9
CHC-CoA: 25.5
Acetyl-CoA: 19.7
inactive: 9.1

IE257_RS37300: pk - mal

PKS_AT (566..863): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 212.1
Methylmalonyl-CoA: 209.4
Methoxymalonyl-CoA: 178.5
2-Methylbutyryl-CoA: 142.1
trans-1,2-CPDA: 114.3
Propionyl-CoA: 112.1
Malonyl-CoA: 107.5
CHC-CoA: 98.1
Isobutyryl-CoA: 96.1
Benzoyl-CoA: 73.1
3-Methylbutyryl-CoA: 54.1
Acetyl-CoA: 52.7
fatty_acid: 43.9
inactive: 38.3

PKS_AT (2093..2386): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.5
inactive: 101.4
Methoxymalonyl-CoA: 59.0
Methylmalonyl-CoA: 51.5
Propionyl-CoA: 39.9
Acetyl-CoA: 18.1
fatty_acid: 16.9
Benzoyl-CoA: 13.6
Isobutyryl-CoA: 12.6
CHC-CoA: 11.8
2-Methylbutyryl-CoA: 10.9
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.8

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_BMVL01000033.1 - Region 1 - ectoine

Download region GenBank file

Download region SVG

Location: 7,835 - 15,455 nt. (total: 7,621 nt) Show pHMM detection rules used

Region on contig edge.

ectoine: ectoine_synt

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

IE257_RS37360: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000858.3	0.49	other (other)	ectoine	Methylobacter marinus
BGC0001778.5	0.43	other (other)	showdomycin	Streptomyces showdoensis
BGC0002097.3	0.41	NRPS, PKS, saccharide	kosinostatin	Micromonospora sp. 28ISP2-46
BGC0000706.3	0.41	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0001073.5	0.40	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0000003.3	0.40	PKS	AF-toxin	Alternaria alternata
BGC0001464.3	0.39	other (other)	tetrabromopyrrole	Pseudoalteromonas sp. PS5
BGC0001463.4	0.38	other (other)	2,3,4,5-tetrabromopyrrole	Pseudoalteromonas piscicida
BGC0002675.2	0.36	PKS	prodigiosin	Pseudoalteromonas rubra
BGC0000258.3	0.36	PKS	prodigiosin	Serratia sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000858.3	0.49	other (other)	ectoine	Methylobacter marinus
BGC0001778.5	0.43	other (other)	showdomycin	Streptomyces showdoensis
BGC0002097.3	0.41	NRPS, PKS, saccharide	kosinostatin	Micromonospora sp. 28ISP2-46
BGC0000706.3	0.41	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0001073.5	0.40	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0000003.3	0.40	PKS	AF-toxin	Alternaria alternata
BGC0001464.3	0.39	other (other)	tetrabromopyrrole	Pseudoalteromonas sp. PS5
BGC0001463.4	0.38	other (other)	2,3,4,5-tetrabromopyrrole	Pseudoalteromonas piscicida
BGC0002675.2	0.36	PKS	prodigiosin	Pseudoalteromonas rubra
BGC0000258.3	0.36	PKS	prodigiosin	Serratia sp.

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

NZ_BMVL01000034.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 13,949 nt. (total: 13,949 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37385: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS37390: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS37395: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001133.4	0.68	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001133.4	0.68	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 13949:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (Gly - Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS37390: Gly - Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (1128..1524): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: HAATFDIAALELQALVGGEVNLYGPTEATVWATS
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2214..2615): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEASVDVTA
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS37395: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (600..1003): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: MQTHFDFSVWEGSQVFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWSVGMVHK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

NZ_BMVL01000035.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 13,167 nt. (total: 13,167 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37400: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS37405: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000307.4	0.69	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001240.4	0.68	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000307.4	0.69	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001240.4	0.68	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 13167:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn - X - Phe - Gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS37405: Asn - X - Phe - Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (25..288): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTVTSVKAVVGGEVNEYGPTETVVGCAV
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (971..1355): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGQGFDAFAWEMALVTGGEFNSFGPAETTVDATL
Nearest Stachelhaus code(s):: Trp DAWSVGSVCK (59% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2038..2428): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: MGHAFDAFAWEMTLVTAGEFNAYGPAETTVDATC
Nearest Stachelhaus code(s):: Phe DAWTVAAVCK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (3111..3508): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: HGVTFDIAALELQGLVGGEVNLYGPTEVTVWATS
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

NZ_BMVL01000037.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 12,079 nt. (total: 12,079 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37465: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

IE257_RS37470: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000396.5	0.70	NRPS	nodularin	Nostoc sp. 73.1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000396.5	0.70	NRPS	nodularin	Nostoc sp. 73.1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 12079:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asp - Val) + (Gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS37465: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (470..858): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: HGVTFDIAALELQALVGGEVNLYGPTEATVWATS
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

IE257_RS37470: Asp - Val

Search NORINE for peptide: strict or relaxed

AMP-binding (808..1211): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YTVQTDLGNTVAKVVLGGEHNHYGPTETTIGVAT
Nearest Stachelhaus code(s):: Asp DLTKVGHIGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1895..2297): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LRNAFDAATFEGWLLAGGDFNGYGPTENTTFTAV
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

NZ_BMVL01000039.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 5,948 nt. (total: 5,948 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37510: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.72	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0002164.2	0.66	NRPS	peramine	Epichloe festucae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.72	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0002164.2	0.66	NRPS	peramine	Epichloe festucae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 5948:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn - Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS37510: Asn - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (25..287): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTVTSVKAVVGGEVNEYGPTETVVGCAV
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (951..1341): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LSGAFDAAVWEGLLLSGGEVNAYGPTEASVCVAT
Nearest Stachelhaus code(s):: Leu DAWLLGAVCK (65% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

NZ_BMVL01000040.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 5,742 nt. (total: 5,742 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001273.3	0.61	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.60	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.60	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.60	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.60	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001160.5	0.59	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001254.3	0.58	PKS	ACT-Toxin II	Alternaria alternata
BGC0002171.2	0.58	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.58	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001273.3	0.61	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.60	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.60	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.60	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.60	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001160.5	0.59	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001254.3	0.58	PKS	ACT-Toxin II	Alternaria alternata
BGC0002171.2	0.58	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.58	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

NRPS/PKS substrate predictions

IE257_RS37515: mmal

PKS_AT (744..1041): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 228.7
Ethylmalonyl-CoA: 226.1
Methoxymalonyl-CoA: 181.7
2-Methylbutyryl-CoA: 158.0
trans-1,2-CPDA: 142.0
Propionyl-CoA: 121.7
Malonyl-CoA: 109.2
CHC-CoA: 100.9
Isobutyryl-CoA: 95.4
Benzoyl-CoA: 81.6
Acetyl-CoA: 62.0
inactive: 36.8
3-Methylbutyryl-CoA: 35.1
fatty_acid: 31.8

Link to PARAS substrate predictor

NZ_BMVL01000045.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 3,226 nt. (total: 3,226 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37610: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 3226:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

IE257_RS37610: X

AMP-binding (10..220): X

nrpys: Asp, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ---A--------KAVLGGEFNHYGPTETTIGVAT
Nearest Stachelhaus code(s):: Asp DLTKVGHIGK (65% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

NZ_BMVL01000049.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 2,036 nt. (total: 2,036 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37660: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001250.4	0.63	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.63	NRPS	epichloenin A	Epichloe festucae
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001250.4	0.63	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.63	NRPS	epichloenin A	Epichloe festucae
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NZ_BMVL01000050.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 1,924 nt. (total: 1,924 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37665: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.61	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.61	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.61	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.61	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.61	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.61	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001249.5	0.59	NRPS	dimethylcoprogen	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.61	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.61	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.61	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.61	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.61	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.61	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001249.5	0.59	NRPS	dimethylcoprogen	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NZ_BMVL01000051.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 1,909 nt. (total: 1,909 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37670: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.60	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002518.3	0.60	NRPS	syringafactin A, syringafactin C	Pseudomonas sp. SZ57

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.60	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002518.3	0.60	NRPS	syringafactin A, syringafactin C	Pseudomonas sp. SZ57

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NZ_BMVL01000052.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 1,894 nt. (total: 1,894 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

IE257_RS37675: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.61	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.61	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.61	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.61	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.61	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001240.4	0.59	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.61	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.61	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.61	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.61	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.61	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001240.4	0.59	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains