LOCUS       NZ_BMVL01000052         1894 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence52, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000052
VERSION     NZ_BMVL01000052.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1894
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000052.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS_motif       complement(<1..37)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37675_0008"
                     /evalue="4.00E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="IE257_RS37675"
                     /protein_end="631"
                     /protein_start="618"
                     /score="19.5"
                     /tool="antismash"
                     /translation="YVIYTSGSTGRPK"
     CDS             complement(<1..1892)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (6-31). E-value: 8e-13.
                     Score: 39.4. Matches aSDomain:
                     nrpspksdomains_IE257_RS37675_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_Dual (10-301). E-value:
                     1.3e-113. Score: 371.1. Matches aSDomain:
                     nrpspksdomains_IE257_RS37675_Condensation_Dual.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE257_RS37675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_189974629.1"
                     /sec_met_domain="Condensation (E-value: 2.1e-49, bitscore:
                     154.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 7.8e-37, bitscore:
                     112.8, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEGGTANVADVYPLAPLQEGLLFHHLLAHDEDDAYVMPVVMEFS
                     SRERLDAFLDALQKVIDRHDILRTGIVWDGLSQPVQAVWRKAELPVHEVALDPNDSDPV
                     QQLLTGVGLSMNLDRAPLISTHIAPVPEDERWVALVRMHHIVQDHTALEVVLAELDAFL
                     IGRGGDLPDPLPFRDFVAQARSGADTEGQERYFAELLGDVTEGTAPFGALDVRGDGSGV
                     VRAQVPFAPELDARLREVSRRLGASAATVMHVAWARALATMSGREDVVFGTVLFGRMNA
                     GAASERVPGPFMNTLPVRVRTGDMGVLDAVSAVRGQLAELLEHEHAPLALAIQASGMTG
                     NSPLFSSLFNYRHNTEPAAEPVAVTSTEPWDGPVGGIRTLFTRELTNYPLAVSVDDDGD
                     AIGLAVDAVSPIDPEAVGVLVRTAAENLVSALELALDGGPQAPLSTVEVLDDARRRQVL
                     VDWNDTAVEVGSSSLSGLFEAQVGRTPGAVAVVFEGVEVSYAELDGRANRLARLLVSRG
                     VGAESVVGVALERGVDMVVALLAVVKAGGAYLPIDPEYPSERVAFMLEDAAPVVVVTSQ
                     AVAGVVPSGVPVVVVDEHAVVSELAGVADGPLGVRVLPSHPVYVIYTSGSTGRPK"
     source          1..1894
                     /culture_collection="JCM:4726"
                     /db_xref="taxon:1895"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces avidinii"
                     /strain="JCM 4726"
                     /submitter_seqid="sequence52"
                     /type_material="type strain of Streptomyces avidinii"
     gene            complement(<1..>1894)
                     /locus_tag="IE257_RS37675"
     region          1..1894
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1894
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1894
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1892](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1892)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(236..277)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37675_0007"
                     /evalue="2.40E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="IE257_RS37675"
                     /protein_end="552"
                     /protein_start="538"
                     /score="17.5"
                     /tool="antismash"
                     /translation="LAVVKAGGAYLPID"
     CDS_motif       complement(878..940)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37675_0006"
                     /evalue="4.00E-06"
                     /label="C67_dual_14fromHMM"
                     /locus_tag="IE257_RS37675"
                     /protein_end="338"
                     /protein_start="317"
                     /score="19.5"
                     /tool="antismash"
                     /translation="LAELLEHEHAPLALAIQASGM"
     aSDomain        complement(989..1861)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37675_Condensation_Dual.
                     1"
                     /domain_subtypes="Condensation_Dual"
                     /evalue="1.30E-113"
                     /label="IE257_RS37675_Condensation_Dual.1"
                     /locus_tag="IE257_RS37675"
                     /protein_end="301"
                     /protein_start="10"
                     /score="371.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVQDH"
                     /tool="antismash"
                     /translation="DVYPLAPLQEGLLFHHLLAHDEDDAYVMPVVMEFSSRERLDAFLD
                     ALQKVIDRHDILRTGIVWDGLSQPVQAVWRKAELPVHEVALDPNDSDPVQQLLTGVGLS
                     MNLDRAPLISTHIAPVPEDERWVALVRMHHIVQDHTALEVVLAELDAFLIGRGGDLPDP
                     LPFRDFVAQARSGADTEGQERYFAELLGDVTEGTAPFGALDVRGDGSGVVRAQVPFAPE
                     LDARLREVSRRLGASAATVMHVAWARALATMSGREDVVFGTVLFGRMNAGAASERVPGP
                     FMNTLPVRVR"
     CDS_motif       complement(995..1090)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37675_0005"
                     /evalue="3.20E-13"
                     /label="C5_dual_260-291"
                     /locus_tag="IE257_RS37675"
                     /protein_end="299"
                     /protein_start="267"
                     /score="42.0"
                     /tool="antismash"
                     /translation="DVVFGTVLFGRMNAGAASERVPGPFMNTLPVR"
     CDS_motif       complement(1331..1369)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37675_0004"
                     /evalue="4.10E-05"
                     /label="C4_dual_169-182"
                     /locus_tag="IE257_RS37675"
                     /protein_end="187"
                     /protein_start="174"
                     /score="16.3"
                     /tool="antismash"
                     /translation="PFRDFVAQARSGA"
     CDS_motif       complement(1403..1468)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37675_0003"
                     /evalue="1.00E-07"
                     /label="C3_dual_135-156"
                     /locus_tag="IE257_RS37675"
                     /protein_end="163"
                     /protein_start="141"
                     /score="24.7"
                     /tool="antismash"
                     /translation="MHHIVQDHTALEVVLAELDAFL"
     CDS_motif       complement(1673..1786)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37675_0002"
                     /evalue="2.00E-20"
                     /label="C2_dual_024-063"
                     /locus_tag="IE257_RS37675"
                     /protein_end="73"
                     /protein_start="35"
                     /score="65.4"
                     /tool="antismash"
                     /translation="YVMPVVMEFSSRERLDAFLDALQKVIDRHDILRTGIVW"
     aSDomain        complement(1799..1873)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37675_NRPS-COM_Nterm.1"
                     /evalue="8.00E-13"
                     /label="IE257_RS37675_NRPS-COM_Nterm.1"
                     /locus_tag="IE257_RS37675"
                     /protein_end="31"
                     /protein_start="6"
                     /score="39.4"
                     /tool="antismash"
                     /translation="ANVADVYPLAPLQEGLLFHHLLAHD"
     CDS_motif       complement(1814..1855)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37675_0001"
                     /evalue="3.20E-07"
                     /label="C1_dual_004-017"
                     /locus_tag="IE257_RS37675"
                     /protein_end="26"
                     /protein_start="12"
                     /score="22.5"
                     /tool="antismash"
                     /translation="YPLAPLQEGLLFHH"
ORIGIN
        1 cttcggccga cccgtcgaac ccgacgtata gatcacatac accggatgcg acggcagtac
       61 ccgcacgccc agcggcccat cagcgacacc cgccaactca ctcacgactg cgtgctcgtc
      121 gaccacgacc accggaaccc cggacggcac cacaccggcc acagcctgcg aagtcaccac
      181 cacgaccggc gcagcatcct ccaacatgaa cgcgacccgc tcggacggat actccggatc
      241 gatcggcaga tacgcaccac ccgccttcac caccgccagc agagcgacca ccatgtcgac
      301 accacgctcc aacgcgacac cgaccaccga ctccgcaccc acaccacgcg acaccaacag
      361 acgcgccaga cgattcgccc gcccatccag ctccgcgtac gaaacctcca caccctcgaa
      421 caccaccgca accgcacccg gagtacgccc cacctgcgcc tcgaacaacc ccgacagaga
      481 agaggaaccg acctccaccg ccgtgtcgtt ccagtccacc aggacctgcc gacgccgggc
      541 gtcgtccagt acctcgaccg tgctcagcgg cgcctgcgga ccaccgtcca gtgccagctc
      601 cagcgccgac accaggttct cggcggccgt acgcaccagc acaccgaccg cttccgggtc
      661 gatcggggag accgcgtcga cggccagtcc gatcgcgtca ccgtcgtcgt cgaccgagac
      721 ggcgagcggg tagttggtca gttcccgggt gaacagcgtg cggatgccac ccaccggccc
      781 gtcccagggc tcggtcgacg tcaccgccac gggctcggcc gccggctcgg tgttgtgccg
      841 gtagttgaac agcgaggaga acaaggggga gttcccggtc atgccgctgg cctggatggc
      901 cagggccaac ggggcgtgct cgtgctccag cagttccgcc agctgaccgc ggaccgcgga
      961 caccgcgtcc aggacgccca tgtcgcccgt acgcacgcgc accggcagcg tgttcatgaa
     1021 gggacccggg acccgctccg aggccgcgcc cgcgttcatc cggccgaaca gcaccgttcc
     1081 gaacaccacg tcctcgcgcc cggacatcgt cgcgagggca cgggcccacg cgacgtgcat
     1141 caccgtcgcc gcactcgcgc cgaggcggcg ggatacctcc cgcagccgcg cgtccagttc
     1201 gggcgcgaac ggaacctggg cgcgcaccac gcccgagccg tcacccctga cgtccagcgc
     1261 accgaacggt gcggtccctt cggtgacatc accgagcagc tcggcgaagt agcgttcctg
     1321 gccctcggtg tccgcaccgc tacgggcctg ggcgacgaag tcgcggaacg gcagggggtc
     1381 cggcaggtct ccgccgcggc cgatgaggaa ggcgtccagt tcggcgagga ccacctcgag
     1441 cgcggtgtgg tcctggacga tgtggtgcat ccgcaccagg gccacccatc gctcgtcctc
     1501 ggggacgggc gcgatgtggg tgctgatgag aggtgcccgg tcgaggttca tcgacaggcc
     1561 gactccggtc agcagctgct ggaccggatc gctgtcgttc gggtccaggg ccacctcgtg
     1621 gacgggcagt tcggccttgc gccagacggc ctggacgggc tgggacaggc cgtcccagac
     1681 gatccccgta cgcaggatgt cgtggcggtc gatcaccttc tgcagcgcgt cgaggaacgc
     1741 gtcgaggcgc tcgcgggagc tgaactccat cacgacgggc atcacgtagg cgtcgtcctc
     1801 gtcatgggcg aggaggtggt ggaagagcag gccttcctgg agcggggcca ggggatagac
     1861 gtccgcgacg ttcgcggtgc ccccctcgac ggtg
//
