LOCUS       NZ_BMVL01000051         1909 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence51, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000051
VERSION     NZ_BMVL01000051.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1909
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     cand_cluster    1..1909
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     gene            complement(<1..1882)
                     /locus_tag="IE257_RS37670"
     CDS             complement(<1..1882)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (0-52). E-value: 1.3e-18. Score:
                     58.9. Matches aSDomain: nrpspksdomains_IE257_RS37670_PCP.1"
                     /NRPS_PKS="Domain: Condensation_Dual (101-392). E-value:
                     2.9e-113. Score: 370.0. Matches aSDomain:
                     nrpspksdomains_IE257_RS37670_Condensation_Dual.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE257_RS37670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_229920914.1"
                     /sec_met_domain="PP-binding (E-value: 1.7e-13, bitscore:
                     37.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 7.1e-52, bitscore:
                     162.4, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.6e-14, bitscore:
                     38.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEVGVDDDFFALGGHSLLAVHLVEVLRKHGVSVSVRALFDTPTP
                     VGLAASAGVEAVAVPANLIPADAVEITPELLPLVDLTAEEIERIVATVEGGAANVADIY
                     PLAPLQEGLLFHHLMAEGGEDAYLMPVVLEMDSPERVEEFARAFQLVVDRHDILRTSFV
                     WEGLREPVQVVWRKAVLPVIEVDLDPAAADPAAALQAAVGLSMDPGRAPLVSVHAAALP
                     DGERRLALLRLHHLVQDHTALEVLLHEVQMFLAGRGDELEPSLPFRDFVAQVRGGVDRA
                     EHERYFAELLGDVTEPTAPYGLVDVRGDGTDVERAVVPLAPEVVAQLRSVARRVGTSAA
                     TVLHVAWARVLAAVSGRDDVVFGTVLFGRMNAGAGADRVAGLYMNTLPVRVRTGELGAL
                     EAVTAMRGQLAGLLEHEHASLALAQQASGLAGNTPVFTSLLNYRHNSGRDDAPEGGGAP
                     EGIRVLSSRERTSYPLGVSVDDNGDSMSLAVDAVTPIDAAAVGALLRTAVERLVPLVEQ
                     ALDGGPDTALGSVHVLAAEELRRLLVEWNDTAAEVAPSTLPELFAAQVARTPEATAVVF
                     EGAEHSYAELDARANRLARLLLSRGVTTGSVVGVRLERGVELVVALLAVLK"
     source          1..1909
                     /culture_collection="JCM:4726"
                     /db_xref="taxon:1895"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces avidinii"
                     /strain="JCM 4726"
                     /submitter_seqid="sequence51"
                     /type_material="type strain of Streptomyces avidinii"
     region          1..1909
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     protocluster    1..1909
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1882](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1882)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(593..658)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37670_0006"
                     /evalue="1.10E-06"
                     /label="C67_dual_14fromHMM"
                     /locus_tag="IE257_RS37670"
                     /protein_end="430"
                     /protein_start="408"
                     /score="21.3"
                     /tool="antismash"
                     /translation="LAGLLEHEHASLALAQQASGLA"
     aSDomain        complement(707..1579)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37670_Condensation_Dual.
                     1"
                     /domain_subtypes="Condensation_Dual"
                     /evalue="2.90E-113"
                     /label="IE257_RS37670_Condensation_Dual.1"
                     /locus_tag="IE257_RS37670"
                     /protein_end="392"
                     /protein_start="101"
                     /score="370.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHLVQDH"
                     /tool="antismash"
                     /translation="DIYPLAPLQEGLLFHHLMAEGGEDAYLMPVVLEMDSPERVEEFAR
                     AFQLVVDRHDILRTSFVWEGLREPVQVVWRKAVLPVIEVDLDPAAADPAAALQAAVGLS
                     MDPGRAPLVSVHAAALPDGERRLALLRLHHLVQDHTALEVLLHEVQMFLAGRGDELEPS
                     LPFRDFVAQVRGGVDRAEHERYFAELLGDVTEPTAPYGLVDVRGDGTDVERAVVPLAPE
                     VVAQLRSVARRVGTSAATVLHVAWARVLAAVSGRDDVVFGTVLFGRMNAGAGADRVAGL
                     YMNTLPVRVR"
     CDS_motif       complement(713..808)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37670_0005"
                     /evalue="2.60E-14"
                     /label="C5_dual_260-291"
                     /locus_tag="IE257_RS37670"
                     /protein_end="390"
                     /protein_start="358"
                     /score="45.4"
                     /tool="antismash"
                     /translation="DVVFGTVLFGRMNAGAGADRVAGLYMNTLPVR"
     CDS_motif       complement(1046..1087)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37670_0004"
                     /evalue="9.50E-05"
                     /label="C4_dual_169-182"
                     /locus_tag="IE257_RS37670"
                     /protein_end="279"
                     /protein_start="265"
                     /score="15.2"
                     /tool="antismash"
                     /translation="PFRDFVAQVRGGVD"
     CDS_motif       complement(1121..1183)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37670_0003"
                     /evalue="5.80E-07"
                     /label="C3_dual_135-156"
                     /locus_tag="IE257_RS37670"
                     /protein_end="254"
                     /protein_start="233"
                     /score="22.4"
                     /tool="antismash"
                     /translation="HHLVQDHTALEVLLHEVQMFL"
     CDS_motif       complement(1391..1504)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37670_0002"
                     /evalue="2.00E-18"
                     /label="C2_dual_024-063"
                     /locus_tag="IE257_RS37670"
                     /protein_end="164"
                     /protein_start="126"
                     /score="59.0"
                     /tool="antismash"
                     /translation="YLMPVVLEMDSPERVEEFARAFQLVVDRHDILRTSFVW"
     CDS_motif       complement(1532..1573)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37670_0001"
                     /evalue="3.10E-07"
                     /label="C1_dual_004-017"
                     /locus_tag="IE257_RS37670"
                     /protein_end="117"
                     /protein_start="103"
                     /score="22.5"
                     /tool="antismash"
                     /translation="YPLAPLQEGLLFHH"
     aSDomain        complement(1727..1882)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37670_PCP.1"
                     /evalue="1.30E-18"
                     /label="IE257_RS37670_PCP.1"
                     /locus_tag="IE257_RS37670"
                     /protein_end="52"
                     /protein_start="0"
                     /score="58.9"
                     /tool="antismash"
                     /translation="MPEVGVDDDFFALGGHSLLAVHLVEVLRKHGVSVSVRALFDTPTP
                     VGLAASA"
ORIGIN
        1 ccttcagcac ggccagcagt gccacgacca gctcgacacc gcgctccagg cggacgccga
       61 ccacggagcc cgtggtgacg ccccgcgaca gcagcagccg ggccaggcgg ttcgcccgag
      121 cgtccagctc cgcgtacgag tgctccgcgc cctcgaagac gaccgcggtc gcctccgggg
      181 tgcgtgccac ctgtgccgcg aacagctcgg ggagcgtcga cggcgcgacc tcggccgcgg
      241 tgtcgttcca ctccaccagc agtcgccgca gctcctcggc agccaggacg tggacgctgc
      301 cgagcgccgt gtccggtccg ccgtcgagcg cctgctcgac caggggaacg agcctctcga
      361 cggccgtgcg taggagcgcc cccacggccg cggcgtcgat cggggtgacg gcgtccaccg
      421 ccagcgacat cgagtcgccg ttgtcgtcga cggacacgcc gagcgggtag ctcgtacgct
      481 cccgcgagga cagcacccgg atgccctccg gagcccctcc gccctcgggc gcgtcgtccc
      541 tgccgctgtt gtggcggtag ttgagcagtg aggtgaacac gggggtgttg cccgcgaggc
      601 cactggcctg ctgggccagg gccagcgagg cgtgctcgtg ctccagcaac ccggccagct
      661 ggccgcgcat cgcggtcacc gcctcgagcg cgcccagttc acccgtgcgc acccgcaccg
      721 ggagcgtgtt catgtagagc ccggccaccc ggtcggcgcc cgcgcccgcg ttcatccggc
      781 cgaagagcac cgtgccgaag accacgtcgt cgcggccgct gaccgcggcc agcacccgcg
      841 cccacgccac gtgcagcacc gtcgccgcac tggtcccgac ccggcgcgcc acggaccgca
      901 gctgtgcgac gacctccggc gcgagcggca ccacggcacg ctccacatcg gtgccgtcac
      961 cgcgtacgtc gaccagcccg tagggggccg tcggctccgt cacgtcgccg agcagctcgg
     1021 cgaagtaccg ctcgtgctcg gcccggtcca caccgccgcg cacctgcgcg acgaagtcac
     1081 ggaacggcag ggagggctcc agctcgtcac cgcgcccggc caggaacatc tgcacctcgt
     1141 gcaggaggac ctcgagggcc gtgtggtcct ggaccaggtg gtgcaggcgc agcagcgcca
     1201 gccggcgctc cccgtccggc agcgcggccg cgtgcacgct gaccagcggc gcccggcccg
     1261 ggtccatcga caatcccacg gccgcctgca gcgcggcggc ggggtccgca gccgcggggt
     1321 ccaggtccac ctcgatcacg ggcagcacgg ccttgcgcca caccacctgg accggctcgc
     1381 gcagcccctc ccacacgaag gacgtccgca ggatgtcatg ccggtccacg accagctgga
     1441 aagcacgggc gaactcctcc acccgctccg gcgagtccat ctccaggact accggcatca
     1501 ggtaggcgtc ctcgccgccc tccgccatca ggtggtggaa gagcaggccc tcctgcagcg
     1561 gcgccagcgg atagatgtcc gccacgttcg ccgcgccgcc ttcgaccgtg gcgacgatcc
     1621 gctcgatctc ctccgcggtc aggtcgacca ggggcagcag ttcgggggtg atctccaccg
     1681 cgtcggcggg aatcagattc gccggtacgg ctacggcctc cacacccgcc gaggccgcga
     1741 gcccgaccgg ggtcggcgtg tcgaacagcg cgcggaccga gaccgacacc ccgtgcttgc
     1801 gcaggacctc gaccaggtgg accgcaagca gggagtggcc tccgagggcg aagaagtcgt
     1861 cgtcgacccc gacttcgggc acccggagga cttccgcgaa ggcctggca
//
