LOCUS       NZ_BMVL01000050         1924 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence50, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000050
VERSION     NZ_BMVL01000050.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1924
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS_motif       complement(<1..37)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37665_0008"
                     /evalue="4.00E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="IE257_RS37665"
                     /protein_end="641"
                     /protein_start="628"
                     /score="19.4"
                     /tool="antismash"
                     /translation="YVIYTSGSTGRPK"
     CDS             complement(<1..1922)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (6-29). E-value: 1.8e-12.
                     Score: 38.3. Matches aSDomain:
                     nrpspksdomains_IE257_RS37665_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_Dual (10-301). E-value:
                     3.4e-109. Score: 356.7. Matches aSDomain:
                     nrpspksdomains_IE257_RS37665_Condensation_Dual.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE257_RS37665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_189974625.1"
                     /sec_met_domain="Condensation (E-value: 3.3e-49, bitscore:
                     153.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-39, bitscore:
                     122.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEGGTANVADVYPLAPLQEGLLFHHLLADGGDDAYVLPSVLEFP
                     SREQLDAFTDALQQVIDRHDIFRTSFVWEGLREPVQVVWRKAVLPVVELTLDAQGTDPV
                     AELVAAVGSSLALGRAPLFSFHAAAVPGGERWLGLVRVHHLVQDHTALEVLLGEVGAFL
                     TGRGGDLPAPLPFRTFVAHARSGNDGAAHEQYFAELLGDVTEPTAPYGLVDIHGAGSDV
                     VREVVPFAPASGARLREVARRLGVSTATVLHVAWARALAAVSGRDDVVFGTILFGRMNA
                     GVGADRVPGPLINTLPVRVRTAELGVLGAVTAMRDQLAHLLEHEHAPLAVAQGASGIAG
                     EAPLFTALFNYRHNTGGNAAEETGENPTGAQVSDADGIRLVFSRERTNYPLAVAVDDDG
                     DRGLSLAVDALASIDGLAVGVLVRTAAENLVSALEVALDGGPEVPLSALQVLDEAGLDR
                     VVREWNDTAVEVGSSSLSGLFEAQVGRTPGAVAVVFEGAEVSYAELDARANRLARLLVS
                     RGVGAESVVGVALERGVDMVVALLAVVKAGGAYLPIDPEYPSERVAFMLEDAAPVAVVT
                     SQALAARVLPDSVAASVVVLDDAATVAELAAAEGGALSDAERRGEVLPSHPVYVIYTSG
                     STGRPK"
     source          1..1924
                     /culture_collection="JCM:4726"
                     /db_xref="taxon:1895"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces avidinii"
                     /strain="JCM 4726"
                     /submitter_seqid="sequence50"
                     /type_material="type strain of Streptomyces avidinii"
     gene            complement(<1..>1924)
                     /locus_tag="IE257_RS37665"
     region          1..1924
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1924
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1924
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1922](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1922)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(260..301)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37665_0007"
                     /evalue="2.40E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="IE257_RS37665"
                     /protein_end="554"
                     /protein_start="540"
                     /score="17.5"
                     /tool="antismash"
                     /translation="LAVVKAGGAYLPID"
     CDS_motif       complement(536..574)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37665_0006"
                     /evalue="8.30E-01"
                     /label="C3_dual_135-156"
                     /locus_tag="IE257_RS37665"
                     /protein_end="462"
                     /protein_start="449"
                     /score="3.0"
                     /tool="antismash"
                     /translation="VLDEAGLDRVVRE"
     CDS_motif       complement(902..970)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37665_0005"
                     /evalue="3.00E-07"
                     /label="C67_dual_14fromHMM"
                     /locus_tag="IE257_RS37665"
                     /protein_end="340"
                     /protein_start="317"
                     /score="23.1"
                     /tool="antismash"
                     /translation="LAHLLEHEHAPLAVAQGASGIAG"
     aSDomain        complement(1019..1891)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37665_Condensation_Dual.
                     1"
                     /domain_subtypes="Condensation_Dual"
                     /evalue="3.40E-109"
                     /label="IE257_RS37665_Condensation_Dual.1"
                     /locus_tag="IE257_RS37665"
                     /protein_end="301"
                     /protein_start="10"
                     /score="356.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHLVQDH"
                     /tool="antismash"
                     /translation="DVYPLAPLQEGLLFHHLLADGGDDAYVLPSVLEFPSREQLDAFTD
                     ALQQVIDRHDIFRTSFVWEGLREPVQVVWRKAVLPVVELTLDAQGTDPVAELVAAVGSS
                     LALGRAPLFSFHAAAVPGGERWLGLVRVHHLVQDHTALEVLLGEVGAFLTGRGGDLPAP
                     LPFRTFVAHARSGNDGAAHEQYFAELLGDVTEPTAPYGLVDIHGAGSDVVREVVPFAPA
                     SGARLREVARRLGVSTATVLHVAWARALAAVSGRDDVVFGTILFGRMNAGVGADRVPGP
                     LINTLPVRVR"
     CDS_motif       complement(1025..1120)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37665_0004"
                     /evalue="6.00E-12"
                     /label="C5_dual_260-291"
                     /locus_tag="IE257_RS37665"
                     /protein_end="299"
                     /protein_start="267"
                     /score="38.0"
                     /tool="antismash"
                     /translation="DVVFGTILFGRMNAGVGADRVPGPLINTLPVR"
     CDS_motif       complement(1433..1495)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37665_0003"
                     /evalue="1.30E-06"
                     /label="C3_dual_135-156"
                     /locus_tag="IE257_RS37665"
                     /protein_end="163"
                     /protein_start="142"
                     /score="21.3"
                     /tool="antismash"
                     /translation="HHLVQDHTALEVLLGEVGAFL"
     CDS_motif       complement(1703..1816)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37665_0002"
                     /evalue="9.10E-20"
                     /label="C2_dual_024-063"
                     /locus_tag="IE257_RS37665"
                     /protein_end="73"
                     /protein_start="35"
                     /score="63.3"
                     /tool="antismash"
                     /translation="YVLPSVLEFPSREQLDAFTDALQQVIDRHDIFRTSFVW"
     aSDomain        complement(1835..1903)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37665_NRPS-COM_Nterm.1"
                     /evalue="1.80E-12"
                     /label="IE257_RS37665_NRPS-COM_Nterm.1"
                     /locus_tag="IE257_RS37665"
                     /protein_end="29"
                     /protein_start="6"
                     /score="38.3"
                     /tool="antismash"
                     /translation="ANVADVYPLAPLQEGLLFHHLLA"
     CDS_motif       complement(1844..1885)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37665_0001"
                     /evalue="3.20E-07"
                     /label="C1_dual_004-017"
                     /locus_tag="IE257_RS37665"
                     /protein_end="26"
                     /protein_start="12"
                     /score="22.5"
                     /tool="antismash"
                     /translation="YPLAPLQEGLLFHH"
ORIGIN
        1 cttcggccga cccgtcgaac ccgacgtata gatcacatac accggatgcg acggcagtac
       61 ttcgccccgg cgctccgcgt cggacagcgc accaccctcg gccgcggcca gttccgccac
      121 cgtggcggca tcatcgagca cgaccaccga cgccgcgacc gagtccggaa ggacccgcgc
      181 ggccagggcc tgcgaggtca ccaccgcgac cggcgcggca tcctccaaca tgaacgcgac
      241 ccgctcggac ggatactccg gatcgatcgg cagatacgca ccacccgcct tcaccaccgc
      301 cagcagagcg accaccatgt cgacaccacg ctccaacgcg acaccgacca ccgactccgc
      361 acccacacca cgcgacacca acagacgcgc cagacgattc gcccgggcgt caagctcggc
      421 gtacgaaacc tccgcaccct cgaacaccac cgcaaccgca cccggagtac gccccacctg
      481 cgcctcgaac aaccccgaca gagaagagga accgacctcc accgccgtgt cgttccactc
      541 gcgcacgacc cggtccagcc cagcttcgtc caggacttgc agtgcactca gcggtacctc
      601 gggcccaccg tccagtgcca cctccagcgc cgacaccagg ttctcggcgg ccgtacgcac
      661 cagcacaccg accgcgaggc cgtctatgga agcgagcgcg tccacggcca gcgacagccc
      721 acggtctccg tcgtcgtcga ccgcgaccgc gagcgggtag ttggtccgct cacgcgagaa
      781 cacgaggcgg atgccgtcgg catcgctcac ctgggccccg gtggggttct ctccggtctc
      841 ctccgccgcg ttgccgccgg tgttgtgccg gtagttgaag agggcggtga acagcggcgc
      901 ctcacccgcg atcccgctgg cgccctgcgc cacggcgagc ggcgcgtgct cgtgctccag
      961 caggtgtgcg agctggtcgc gcatcgcggt gaccgcgccc agcacaccga gttcggcggt
     1021 gcgcacgcgt accggaagcg tgttgatcag cggaccgggg acccggtcgg cgccgacccc
     1081 cgcgttcatc cggccgaaca ggatcgtgcc gaagaccacg tcgtcacggc cggagaccgc
     1141 ggccagtgcc cgggcccacg ccacgtgcag aacggtggct gtgctcacgc ccagtcgacg
     1201 tgccacctcg cgcagccgcg ccccgctggc cggagcgaac ggtacgacct cacgcaccac
     1261 gtctgagccg gctccgtgga tgtccaccag gccgtagggg gcagtcggct cggtcacgtc
     1321 gccgagcagc tccgcgaagt actgctcgtg cgccgccccg tcgttgccgc tacgggcgtg
     1381 ggcgacgaag gtccggaagg gcagcggcgc aggcaggtca ccgccccggc cggtgaggaa
     1441 cgcgccgact tcgccgagga gcacctccag cgcggtgtgg tcctgcacca ggtggtgcac
     1501 ccgcacgagg cccagccagc gctcgccacc cggtacggcc gccgcgtgga agctgaacag
     1561 gggcgcccgg ccgagagcca gggaggatcc gaccgctgcg accagctcgg cgaccggatc
     1621 agtgccctgc gcgtcgagcg tcagctcgac gaccggcagg acggccttgc gccagacgac
     1681 ctggaccggt tcgcgcagtc cttcccagac gaacgaggtc cggaaaatgt cgtgccggtc
     1741 gatcacctgc tgcagcgcgt ccgtgaaggc atccagctgc tcgcgcgagg ggaactcgag
     1801 caccgaaggc agcacgtagg cgtcgtcacc gccgtcggcg aggaggtggt ggaagagcag
     1861 gccctcctgg agcggggcca ggggatagac gtccgcgacg ttcgcggtgc ccccctcgac
     1921 ggtg
//
