LOCUS       NZ_BMVL01000049         2036 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence49, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000049
VERSION     NZ_BMVL01000049.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2036
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..2036
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1993](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1993)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..1993)
                     /locus_tag="IE257_RS37660"
     CDS             complement(<1..1993)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (145-213). E-value: 3.7e-27. Score:
                     86.3. Matches aSDomain: nrpspksdomains_IE257_RS37660_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (227-528). E-value:
                     4.9e-113. Score: 369.0. Matches aSDomain:
                     nrpspksdomains_IE257_RS37660_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE257_RS37660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_229920913.1"
                     /sec_met_domain="AMP-binding (E-value: 3.3e-24, bitscore:
                     71.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.2e-20, bitscore:
                     59.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.6e-68, bitscore:
                     217.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASPYGTGERLYRTGDIARWTADGQVEYLGRVDEQVKVRGFRIEP
                     GEVQAVVAAHPQVGQAAVVAREDVPGDKRLVAYVVANGEGAELASAIKDFVAGRLPEYM
                     VPSAVVVLDALPLSVNGKLDRKALPAPEYTTGAGRKAGNVQEEILSAAFADVLGLDDVG
                     VDDDFFALGGHSLLAMRLVSRVRSVLGVEMPLRTLFEAPTVARLAARLTGADRARIALT
                     SGVRPERVPLSFAQRRLWFLNQLEGPSATYNVPAVVSLSSRVDRDALGAAFRDVLGRHE
                     VLRTVFPVVDGEPYQRIVGIDDLDWELSVQDMAPADLVPAVERTLEHAFDLSSEIPFRA
                     WLFEASPDERVLVVVMHHVASDGWSKGPLARDLSVAYAARSEGRAPEWDALPVQYADYT
                     LWQREILGDEQDEESVISRQIAYWRDALSGSPEELELPFDRPRPMVASHRGHQVEVEVP
                     AVVHARLVEVARVEGVTPFMVLQASLAVLLSKLGAGSDIPIGSANAGRTDEALDDLIGF
                     FINTLVVRTDLSGDPTFREVLGRVRERTLSALGHQDVPFEKLVEELSPARSMARHPLFQ
                     VVLTKQNTVDVVLDLPGLQAGGPTETGSAQADAVEPEPAVSGAAAKFDLDVLVGEVFDA
                     DGRPAGVRGTVTVSADLFDPVWAGRIASA"
     source          1..2036
                     /culture_collection="JCM:4726"
                     /db_xref="taxon:1895"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces avidinii"
                     /strain="JCM 4726"
                     /submitter_seqid="sequence49"
                     /type_material="type strain of Streptomyces avidinii"
     region          1..2036
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2036
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(257..370)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37660_0007"
                     /evalue="7.50E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE257_RS37660"
                     /protein_end="579"
                     /protein_start="541"
                     /score="63.4"
                     /tool="antismash"
                     /translation="RERTLSALGHQDVPFEKLVEELSPARSMARHPLFQVVL"
     aSDomain        complement(410..1312)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37660_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.90E-113"
                     /label="IE257_RS37660_Condensation_LCL.1"
                     /locus_tag="IE257_RS37660"
                     /protein_end="528"
                     /protein_start="227"
                     /score="369.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHVASDG"
                     /tool="antismash"
                     /translation="ERVPLSFAQRRLWFLNQLEGPSATYNVPAVVSLSSRVDRDALGAA
                     FRDVLGRHEVLRTVFPVVDGEPYQRIVGIDDLDWELSVQDMAPADLVPAVERTLEHAFD
                     LSSEIPFRAWLFEASPDERVLVVVMHHVASDGWSKGPLARDLSVAYAARSEGRAPEWDA
                     LPVQYADYTLWQREILGDEQDEESVISRQIAYWRDALSGSPEELELPFDRPRPMVASHR
                     GHQVEVEVPAVVHARLVEVARVEGVTPFMVLQASLAVLLSKLGAGSDIPIGSANAGRTD
                     EALDDLIGFFINTLVVRTDL"
     CDS_motif       complement(419..508)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37660_0006"
                     /evalue="8.30E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE257_RS37660"
                     /protein_end="525"
                     /protein_start="495"
                     /score="40.7"
                     /tool="antismash"
                     /translation="DIPIGSANAGRTDEALDDLIGFFINTLVVR"
     CDS_motif       complement(776..814)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37660_0005"
                     /evalue="6.70E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE257_RS37660"
                     /protein_end="406"
                     /protein_start="393"
                     /score="18.5"
                     /tool="antismash"
                     /translation="QYADYTLWQREIL"
     CDS_motif       complement(863..928)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37660_0004"
                     /evalue="1.40E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE257_RS37660"
                     /protein_end="377"
                     /protein_start="355"
                     /score="27.3"
                     /tool="antismash"
                     /translation="MHHVASDGWSKGPLARDLSVAY"
     CDS_motif       complement(1130..1246)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37660_0003"
                     /evalue="3.30E-15"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE257_RS37660"
                     /protein_end="288"
                     /protein_start="249"
                     /score="48.4"
                     /tool="antismash"
                     /translation="ATYNVPAVVSLSSRVDRDALGAAFRDVLGRHEVLRTVFP"
     CDS_motif       complement(1265..1297)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37660_0002"
                     /evalue="2.00E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="IE257_RS37660"
                     /protein_end="243"
                     /protein_start="232"
                     /score="10.7"
                     /tool="antismash"
                     /translation="SFAQRRLWFLN"
     aSDomain        complement(1355..1558)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37660_PCP.1"
                     /evalue="3.70E-27"
                     /label="IE257_RS37660_PCP.1"
                     /locus_tag="IE257_RS37660"
                     /protein_end="213"
                     /protein_start="145"
                     /score="86.3"
                     /tool="antismash"
                     /translation="EEILSAAFADVLGLDDVGVDDDFFALGGHSLLAMRLVSRVRSVLG
                     VEMPLRTLFEAPTVARLAARLTG"
     CDS_motif       complement(1850..1912)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37660_0001"
                     /evalue="1.50E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="IE257_RS37660"
                     /protein_end="48"
                     /protein_start="27"
                     /score="36.2"
                     /tool="antismash"
                     /translation="YLGRVDEQVKVRGFRIEPGEV"
ORIGIN
        1 acgccgacgc gatccgcccg gcccacaccg gatcgaacaa atccgccgac accgtcacgg
       61 ttccccgtac accggcggga cggccgtccg cgtcgaacac ctcgccgacc agtacatcga
      121 ggtcgaactt cgccgcggcg ccggagacgg ccggctcggg ctccaccgcg tcggcctgcg
      181 ccgatccggt ctccgtcggg ccgccggcct gcagaccggg caggtcgagc acgacgtcca
      241 cggtgttctg cttggtgagg acgacctgga agagggggtg tcgggccatc gagcgggccg
      301 gtgacagttc ctccaccagc ttctcgaacg gcacgtcctg gtgtcccagg gccgacagcg
      361 tccgctcccg gacccggccc agcacctcac ggaacgtcgg atcacccgac agatccgtcc
      421 gcaccaccag cgtgttgatg aagaacccga tcaggtcgtc cagcgcctcg tccgtccgcc
      481 cggcgttcgc cgaaccgatc ggaatgtccg accccgcccc cagcttggag agcagcaccg
      541 ccagcgacgc ctgcagcacc atgaacgggg tcacaccctc gacccgcgcc acctccacca
      601 accgcgcatg cacgaccgcc ggaacctcga cctccacctg gtgtccgcgg tgcgaggcga
      661 ccatcggacg cggccgatcg aacggcagct ccagctcctc cggcgaaccg gacaacgcgt
      721 cacgccagta ggcgatctga cgcgagatca cactctcctc gtcctgctcg tcgccgagga
      781 tctcacgctg ccagagggtg tagtcggcgt actggaccgg cagcgcgtcc cactcgggcg
      841 cccggccctc gctccgcgcc gcgtaggcga cggacaggtc ccgcgccagc gggcccttcg
      901 accagccgtc actggctacg tggtgcatca ccacgacgag cacccgctca tccgggctcg
      961 cctcgaacag ccaggcgcgg aaggggattt ccgatgacag gtcgaaggcg tgctccaggg
     1021 tgcgctcgac ggccggcacg aggtcggccg gggccatgtc ctggacggac agctcccagt
     1081 ccaggtcgtc gatgccgacg atccgctggt acggctcgcc gtcgacgacg gggaacacgg
     1141 tgcggagcac ctcgtgccgg ccgagcacgt cacggaacgc agcgccgagg gcgtcgcggt
     1201 ccactcggct cgacagcgac accactgccg gcacgttgta ggtcgcgctg gggccttcga
     1261 gctggttgag gaaccacagg cggcgctgcg cgaacgacag cggcacccgc tcggggcgga
     1321 cacccgaggt cagggcgatc cgggcccggt ccgcacccgt cagccgggcc gccaggcgcg
     1381 ccaccgtcgg ggcctcgaac agcgtccgca gcggcatctc gacgcccagg acggaccgca
     1441 cccggctcac caggcgcatc gccaggagcg agtggccgcc cagcgcgaag aagtcgtcgt
     1501 ccacgccgac gtcgtccaga ccgagtacgt cggcgaacgc ggcgctcagg atctcctcct
     1561 ggacgttccc ggccttccgg ccggcgcccg tcgtgtattc gggcgcgggc agggccttgc
     1621 ggtccagctt cccgttcacc gacagcggca gcgcgtccag aacgacgacc gccgacggaa
     1681 ccatgtactc gggaagacga cccgcgacga agtccttgat cgcagaggcg agttcggcgc
     1741 cttcgccgtt ggcgacgacg taggcgacca gacgcttgtc acccggcaca tcctcacggg
     1801 cgaccacagc cgcctgcccg acctgcggat gggccgcgac cacggcctgg acctcacccg
     1861 gctcgatccg gaatccacgg accttgacct gctcgtccac gcggcccagg tactccacct
     1921 gaccatcggc cgtccaccgc gcgatgtcgc ccgtacggta aagacgctca ccggtgccgt
     1981 acggagaagc cacgaaacgc tccgccgaca gacccggacg acccagatac ccacga
//
