LOCUS       NZ_BMVL01000045         3226 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence45, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000045
VERSION     NZ_BMVL01000045.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3226
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..3224)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: AMP-binding (10-220). E-value: 1e-50.
                     Score: 164.5. Matches aSDomain:
                     nrpspksdomains_IE257_RS37610_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (332-400). E-value: 3.2e-28. Score:
                     89.7. Matches aSDomain: nrpspksdomains_IE257_RS37610_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (414-716). E-value:
                     2.1e-114. Score: 373.5. Matches aSDomain:
                     nrpspksdomains_IE257_RS37610_Condensation_LCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE257_RS37610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_189974616.1"
                     /sec_met_domain="AMP-binding (E-value: 3.6e-66, bitscore:
                     209.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.8e-18, bitscore:
                     53.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.1e-73, bitscore:
                     233.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYALLQAQATDLGNTVVFASLTTGGELHVLEEGAVTDPAAVSSYL
                     VEHGIDFLKAVPSHLAALASVTGVEGVLPGRSLVLGGEAAAPALVRELVEHGGDGVVFN
                     HYGPTETTIGVATTRLEVSDADAGLVPVGAPVANTRFYVLDASLRPVPVGVVGELYVAG
                     VQLARGYVKRTALTAERFVANPFEPGLRMYRTGDRARWTEDGRVVFQGRADDQVKIRGY
                     RVEPGEVQVAVTAHPLVAQAAVVAREDVPGDTRLVAYAVPMDEDGDLDGLPSAVREFMA
                     RRLPEHMVPAAVVVLDALPLTSNGKLDRKALPAPEYGVTTAGSGRRAANLREELLCLAF
                     ADVLGLENVGVDDDFFELGGHSLLAVRLVSRIRTVLGIETEIRTLFEEPTVAQLAARLA
                     GAESARAPLTRMDRPERLPLSYGQQRLWFINQLEDASASYNIPVVLHLSGEIDREALDA
                     AFRDVIERHAVLRTVFPVVDGEPYQRILGLDELDWEVTTVAVAAADLQDEVEKTAAYPF
                     DLTSEVPIRASLFEAGEGTDPVLAVVLHHIARDGWSTALLTRDFSLAYAARSTGRAPEW
                     TPLPVQYADYAIWQRDLLGDEERPDSLISRQIDYWKHTLSGIPEELDLPFDKNRPATSS
                     QQGHSVQLEVPADVHARLAEVARAEGATMFMVLQSALAVLLNRLGAGTDIPIGTANAGR
                     TDEALDDLVGFFINTLVIRTDLSGDPTFADVLARAREAGLSAFAHQEVPFEKLVEQLAP
                     SRSMARHPLFQVSLTLQNNKEAELDLAGARMTDAPTSTVWAKYDVQVGLGERRDAEGAP
                     AGLHGGIIGSADLFEPASVELFAQRLVRVLAAVAADPRVPLSAVDILDGDERRRVVTEW
                     NATEVDLGSGLVPGLFGARVVGAPDAVAVVADGVEVSFAELDVRANRLARFLVGQGVGA
                     ESVVGVCLPRGVDAVVAILAVWKAGAAFLPIDPGYPVDRISFMLADSGVVLTLTDEEIL
                     GDLPAGRARMVALDSTLMGVQLSMLEDTAPEVVTRSEGLAYVIYTSGSTGRPKGVAVTH
                     GGLANYAQWAARSYGAAEGGAPLHSSL"
     source          1..3226
                     /culture_collection="JCM:4726"
                     /db_xref="taxon:1895"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces avidinii"
                     /strain="JCM 4726"
                     /submitter_seqid="sequence45"
                     /type_material="type strain of Streptomyces avidinii"
     gene            complement(<1..>3226)
                     /locus_tag="IE257_RS37610"
     region          1..3226
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3226
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3226
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:3224](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3224)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(80..139)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37610_0009"
                     /evalue="5.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="IE257_RS37610"
                     /protein_end="1048"
                     /protein_start="1028"
                     /score="34.1"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVAVTH"
     CDS_motif       complement(338..379)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37610_0008"
                     /evalue="1.70E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="IE257_RS37610"
                     /protein_end="962"
                     /protein_start="948"
                     /score="18.0"
                     /tool="antismash"
                     /translation="LAVWKAGAAFLPID"
     CDS_motif       complement(917..1024)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37610_0007"
                     /evalue="3.80E-19"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE257_RS37610"
                     /protein_end="769"
                     /protein_start="733"
                     /score="61.1"
                     /tool="antismash"
                     /translation="LSAFAHQEVPFEKLVEQLAPSRSMARHPLFQVSLTL"
     aSDomain        complement(1076..1981)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37610_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.10E-114"
                     /label="IE257_RS37610_Condensation_LCL.1"
                     /locus_tag="IE257_RS37610"
                     /protein_end="716"
                     /protein_start="414"
                     /score="373.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIARDG"
                     /tool="antismash"
                     /translation="ERLPLSYGQQRLWFINQLEDASASYNIPVVLHLSGEIDREALDAA
                     FRDVIERHAVLRTVFPVVDGEPYQRILGLDELDWEVTTVAVAAADLQDEVEKTAAYPFD
                     LTSEVPIRASLFEAGEGTDPVLAVVLHHIARDGWSTALLTRDFSLAYAARSTGRAPEWT
                     PLPVQYADYAIWQRDLLGDEERPDSLISRQIDYWKHTLSGIPEELDLPFDKNRPATSSQ
                     QGHSVQLEVPADVHARLAEVARAEGATMFMVLQSALAVLLNRLGAGTDIPIGTANAGRT
                     DEALDDLVGFFINTLVIRTDL"
     CDS_motif       complement(1085..1174)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37610_0006"
                     /evalue="1.30E-12"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE257_RS37610"
                     /protein_end="713"
                     /protein_start="683"
                     /score="40.1"
                     /tool="antismash"
                     /translation="DIPIGTANAGRTDEALDDLVGFFINTLVIR"
     CDS_motif       complement(1442..1480)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37610_0005"
                     /evalue="4.70E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE257_RS37610"
                     /protein_end="594"
                     /protein_start="581"
                     /score="19.0"
                     /tool="antismash"
                     /translation="QYADYAIWQRDLL"
     CDS_motif       complement(1529..1594)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37610_0004"
                     /evalue="6.20E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE257_RS37610"
                     /protein_end="565"
                     /protein_start="543"
                     /score="25.2"
                     /tool="antismash"
                     /translation="LHHIARDGWSTALLTRDFSLAY"
     CDS_motif       complement(1799..1915)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37610_0003"
                     /evalue="1.10E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE257_RS37610"
                     /protein_end="475"
                     /protein_start="436"
                     /score="56.4"
                     /tool="antismash"
                     /translation="ASYNIPVVLHLSGEIDREALDAAFRDVIERHAVLRTVFP"
     aSDomain        complement(2024..2227)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37610_PCP.1"
                     /evalue="3.20E-28"
                     /label="IE257_RS37610_PCP.1"
                     /locus_tag="IE257_RS37610"
                     /protein_end="400"
                     /protein_start="332"
                     /score="89.7"
                     /tool="antismash"
                     /translation="EELLCLAFADVLGLENVGVDDDFFELGGHSLLAVRLVSRIRTVLG
                     IETEIRTLFEEPTVAQLAARLAG"
     aSModule        complement(2024..3193)
                     /complete
                     /domains="nrpspksdomains_IE257_RS37610_AMP-binding.1"
                     /domains="nrpspksdomains_IE257_RS37610_PCP.1"
                     /locus_tags="IE257_RS37610"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2537..2599)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37610_0002"
                     /evalue="2.30E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="IE257_RS37610"
                     /protein_end="229"
                     /protein_start="208"
                     /score="35.7"
                     /tool="antismash"
                     /translation="FQGRADDQVKIRGYRVEPGEV"
     aSDomain        complement(2564..3193)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37610_AMP-binding.1"
                     /evalue="1.00E-50"
                     /label="IE257_RS37610_AMP-binding.1"
                     /locus_tag="IE257_RS37610"
                     /protein_end="220"
                     /protein_start="10"
                     /score="164.5"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="DLGNTVVFASLTTGGELHVLEEGAVTDPAAVSSYLVEHGIDFLKA
                     VPSHLAALASVTGVEGVLPGRSLVLGGEAAAPALVRELVEHGGDGVVFNHYGPTETTIG
                     VATTRLEVSDADAGLVPVGAPVANTRFYVLDASLRPVPVGVVGELYVAGVQLARGYVKR
                     TALTAERFVANPFEPGLRMYRTGDRARWTEDGRVVFQGRADDQVKIR"
     CDS_motif       complement(2681..2770)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37610_0001"
                     /evalue="5.50E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="IE257_RS37610"
                     /protein_end="181"
                     /protein_start="151"
                     /score="50.6"
                     /tool="antismash"
                     /translation="PVGVVGELYVAGVQLARGYVKRTALTAERF"
     misc_feature    complement(3120..3122)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 cagcgacgaa tgcagcggag caccaccctc ggcggcgccg tatgaacgag cagcccactg
       61 ggcgtagttg gcgagcccgc cgtgcgtgac ggccacgccc ttgggacgac ccgtggagcc
      121 cgacgtatag atcacatacg ccagaccctc ggaacgggtc accacctcgg gagcggtgtc
      181 ctcaagcatc gacaactgga cgcccatcag cgtcgagtcc agcgcaacca tccgcgcacg
      241 acccgccgga agatcaccca ggatctcctc atccgtcagc gtcaacacca caccactgtc
      301 cgcgagcatg aacgagatcc gatccaccgg ataaccagga tcgatcggca ggaacgcagc
      361 ccccgccttc cacaccgcaa gaatcgccac cacggcatcc acaccacgcg gcaggcacac
      421 acccaccacc gactccgcac ccacaccctg acccaccagg aaccgcgcca gccgattcgc
      481 ccgcacatcc aactccgcga acgacacctc aacaccatcg gcaaccacag ccaccgcatc
      541 cggagccccc accacccgcg ccccgaacaa ccccggcacc aaacccgaac cgaggtctac
      601 ttcagtggcg ttccactccg tgacgacccg acgacgctcg tccccatcga ggatgtccac
      661 cgcgctcagc ggcacgcgcg ggtcagcagc caccgcggcc aggacgcgca ccaggcgctg
      721 ggcgaagagc tcgaccgacg ccggctcgaa gaggtccgcg gagccgatga tcccgccgtg
      781 cagaccggcc ggagcgccct cggcatcgcg gcgctcgccg aggccgacct ggacgtcgta
      841 cttggcccac acggtcgacg tcggtgcatc agtcatacgg gcacccgcga gatccagttc
      901 cgcctccttg ttgttctgca gcgtcagcga cacctggaac aaggggtggc gagccatgga
      961 gcggctcggt gcgagctgtt ccacgagctt ctcgaagggg acttcctggt gggcgaaggc
     1021 ggacagtccc gcctcacggg cccgtgccag gacgtcggcg aaggtcgggt caccggacag
     1081 gtccgtccga atgacgaggg tgttgatgaa gaagccgacc aggtcgtcga gcgcctcgtc
     1141 cgtacgtccc gcgttggcgg tgccgatcgg gatgtcggtg cccgcaccca ggcggttcag
     1201 caggacggcc agcgccgact gcaggaccat gaacatggtc gcgccttcgg cgcgcgccac
     1261 ctcggcgagc cgggcgtgca cgtccgcggg aacctccagc tggacgctgt ggccctgctg
     1321 ggaggaggtg gccgggcggt tcttgtcgaa cgggaggtcc agttcctcgg ggatgccgga
     1381 cagcgtgtgc ttccagtagt cgatctgacg cgagatgagg ctgtcgggcc gctcctcgtc
     1441 gccgagcagg tcgcgctgcc atatggcgta gtccgcgtac tgcaccggca gcggcgtcca
     1501 ttccggtgcc cggcccgtgg agcgcgccgc gtaggcgagc gagaagtcgc gcgtcagcag
     1561 cgccgtcgac cagccgtccc tggcgatgtg gtgcaggacc acggccagta cgggatcggt
     1621 gccctcgcct gcttcgaaca gcgaggcgcg gatcggcacc tcggaggtga ggtcgaaggg
     1681 gtatgcggct gtcttctcca cctcgtcctg caggtccgcc gcggccacgg cgacggtcgt
     1741 gacctcccag tccagctcgt cgagaccgag gatccgctgg tagggctcgc cgtcgacgac
     1801 ggggaagacg gtgcggagca ccgcgtgccg ttcgatcacg tcgcggaagg ccgcgtccag
     1861 cgcctcgcgg tcgatctccc cggacaggtg gagcaccacc gggatgttgt acgacgcgct
     1921 cgcgtcctcc agctggttga tgaaccagag gcgctgctga ccgtacgaga gcgggaggcg
     1981 ctcggggcgg tccatccgcg tgagcggagc acgggcgctc tcggcccctg ccagtcgggc
     2041 ggcgagctgg gcgacggtcg gctcttcgaa gagcgtgcgg atctcggtct cgatgccgag
     2101 gacggtgcgg atccggctga cgagccggac cgcgagcagg gagtgtccgc cgagttcgaa
     2161 gaagtcgtcg tccacgccga cgttttcgag cccgaggaca tcggcgaagg cgaggcagag
     2221 cagctcctcc cgcaggttcg ccgcccggcg gccgctgccg gccgtcgtca cgccgtactc
     2281 aggtgcgggc agggccttgc ggtcgagctt cccgttggac gtcagcggca gcgcgtccag
     2341 gacgaccacg gccgcgggca ccatgtgctc gggcaaccgc cgggccatga actcccttac
     2401 cgcggacgga agtccgtcga ggtcgccgtc ctcgtccatc ggcaccgcat aggccaccag
     2461 gcgcgtgtcg cccggtacgt cctcgcgggc caccaccgct gcctgcgcga cgagcgggtg
     2521 cgcggtcacg gcgacctgga cctcacccgg ctcgacgcgg tagccgcgga tcttgacctg
     2581 gtcgtcggcg cggccctgga agaccactcg gccgtcctcg gtccagcggg cccggtcacc
     2641 cgtccgatac atccgcaggc cgggctcgaa cgggttcgcg acgaaccgct cggccgtcag
     2701 cgcggtccgc ttgacataac cccgcgccag ctgaacaccc gccacataca actcacccac
     2761 aacaccgaca ggaaccggcc gcagcgaagc atccaacacg tagaaccggg tgttcgccac
     2821 cggagcaccc accggcacca gcccggcatc cgcgtcggag acctccagtc gggtcgtcgc
     2881 cacaccgatc gtggtctcgg tcggcccgta gtggttgaac accaccccgt caccgccgtg
     2941 ctcgaccagc tcccgcacca acgccggagc agccgcctca ccacccagaa ccagcgaacg
     3001 ccccggaagg acaccctcca cgccggtgac cgaagccagc gccgccaggt gcgagggaac
     3061 cgccttcagg aaatcgatcc cgtgctcgac cagatacgag gacaccgccg ccggatcggt
     3121 aaccgcaccc tcctccagga cgtgcaactc accacccgtg gtcaggctcg cgaacaccac
     3181 cgtgttcccc aggtccgtcg cctgcgcctg aagcagcgca taccgg
//
