LOCUS       NZ_BMVL01000040         5742 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence40, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000040
VERSION     NZ_BMVL01000040.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5742
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..5738)
                     /NRPS_PKS="Domain: PKS_PP (102-188). E-value: 8e-30. Score:
                     95.0. Matches aSDomain:
                     nrpspksdomains_IE257_RS37515_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (210-635). E-value:
                     5.8e-185. Score: 607.1. Matches aSDomain:
                     nrpspksdomains_IE257_RS37515_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (744-1041). E-value: 4.6e-108.
                     Score: 353.0. Matches aSDomain:
                     nrpspksdomains_IE257_RS37515_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (1108-1270). E-value: 5.3e-58.
                     Score: 188.0. Matches aSDomain:
                     nrpspksdomains_IE257_RS37515_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_ER (1585-1891). E-value: 7.1e-109.
                     Score: 355.6. Matches aSDomain:
                     nrpspksdomains_IE257_RS37515_PKS_ER.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010984324.1"
                     /locus_tag="IE257_RS37515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189974585.1"
                     /sec_met_domain="PP-binding (E-value: 2.6e-14, bitscore:
                     39.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 1.5e-232, bitscore:
                     758.0, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.7e-98, bitscore:
                     315.3, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 7.6e-110, bitscore:
                     353.0, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_N (E-value: 4.9e-09, bitscore: 22.4,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.2e-17, bitscore:
                     49.5, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWGFWAQRSGMTGHLADEEAVMRRMAGAGVLPISEEQGLALFDAA
                     VALDAPVCVPMPLDLAALRTQAGAGTLPPLLRGLVRAPARRAAAAPGTAGSPAFAAELA
                     ALPQAERTRRLLDLVRTHAATALGYPTSEGLDAKRTFRELGFDSLAAIELRNGVGGATG
                     LRLPATLVFDHPTPSAVVDFLLGELTGGVQARPAARPAVSASGADEPIAIVGMACRYPG
                     GVSSPEELWELVLSGRDAIAGMPEDRGWDVERLYDPELTRPGTSYVRQGGFLYDAADFD
                     PEFFGISPREALAMDPQQRLLLETAWEALEHAGIDPHAVRGSRTGVFTGMMYHDYATDR
                     AELPEEAEGFIGTGSAGSVASGRIAFTLGLEGPALTIDTACSSSLVALHLAAQALRSGE
                     CDLALAGGVTVMSTPAVFVELSRQGGLSPDGRCRSFADGADGTGWGEGAGLLLVERLSD
                     ARRNGHPVLAIVRGTAVNQDGASNGLTAPNGPAQQRVIEEALDNAGLGLGDVDVVEAHG
                     TGTTLGDPIEAQALLATYGRDRPAGRPLRLGSVKSNMGHTQAAAGAAGIIKMVMAMRHG
                     VMPKTLHVDSPSSQIDWTSGGVELLTEAQEWPADGAPRRAGVSSFGISGTNAHVIIEQP
                     PAPAPVAAVPAAPEVVPWVLSGHDRAALYAQAEQLVAHLEAHPELSPADVGRTLTGRAA
                     LPHRAVVLGADRDALLSGASGLAGRVGDPDAALPPGVAEGSVLGDDRVVFVFPGQGAQW
                     AGMAAELLDSSPVFRARVQECEEALSPYVDWSLLAVLRQEPGTPPWDRVDVVQPALWAV
                     MVSLAEVWRSYGVEPDAVIGHSQGEIAAACVCGALDLGDGARIVALRAKAIGSALAGRG
                     GMVSVALPSAAARDLIAGWPGTISVASVNGPVSTVVAGEPGALDELLARCAEDGVRARR
                     IPVDYASHTAQVEEIEADLAAELKGIAPRSSSIPFFSTLTGGTLDTAELDAGYWYRNLR
                     HTVEFEGATRAALAQGFTVFAEISPHPVLVPSVQDSIDDAGAAAAAVGSLRREDGGLRR
                     MLTSVAEAGVLGVDIDWTPVFAGAARQVELPTYAFQRRRFWLEGRRGAGDLTATGLESA
                     DHPLLGAAVVMAGGEGVVLTGLLSGRTHPWLLDHAVSGTVLLPGTAFVDLAIRAGDHLG
                     HPHLEELTLQAPLLLGTGPDDDVTVQVRATPDAGADGCTVTVHSRTGDGDWTLHATGTL
                     GQEAPAEPVPDAAWPPAGAEPVALDGVYDELAEGGYHYGPAFRGLEAVWRRDGELFAEI
                     RLPETEREEAARFGVHPALLDSALHAMAVAGDQESGVKLPFAWTGVHLYATGATAARVR
                     LTPAGDQLSLLLTDDTGRPVVAVRSLVTRPAAIGQTSGGALEEALLHLDWAVLPAGAEG
                     DGAVPYTLVGEDPFGLADGAERVLPDLAALAADGPVPDTVVTCLAPAGSGDPVTAAHAA
                     AQETLERVRAWLADERFATSRLVLVTTGAVAAGDAEDVTDLPHATSWGLVTSAQTENPD
                     RFGLVDLDGRPESRDAFPAALEGSEPRIAVRRGTVTAPRLARARSHSALLPPSGGVPWR
                     LESTGSGTIENLALVPCPEVLDPLGPGQVRIAVHAVGMNFRDVVVALGVVTTQKGIGGD
                     VAGVVMETGSGVTNVAVGDRVLGLCSDSFGPVAVCDHRFLTPMPDGWSYEQAATIPITH
                     LTAYYGLVDLAAVQQGESVLVHAAAGGVGIAAVQLARHLGAEVYGTASPGKWQTLRDHG
                     LDAPHIANSRTLEFEQWFMETSAGCGVDVVLDCLAGEFVDAGLRLQPRGGRFLEMGKTD
                     KRDPAQVAEAYPGVAYQAYDLMEAGPDRIQEMLVAVMDLYRAGALVPPPITTYDVRRAR
                     DAMRDLSQAKLVGKAVLTVPQGIDPEGTALITGGTGTLG"
     source          1..5742
                     /culture_collection="JCM:4726"
                     /db_xref="taxon:1895"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces avidinii"
                     /strain="JCM 4726"
                     /submitter_seqid="sequence40"
                     /type_material="type strain of Streptomyces avidinii"
     gene            complement(<1..>5742)
                     /locus_tag="IE257_RS37515"
     region          1..5742
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..5742
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5742
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:5738](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..5738)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        complement(64..981)
                     /ASF="ER domain putatively catalyzing 2R-configuration
                     product formation"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37515_PKS_ER.1"
                     /evalue="7.10E-109"
                     /label="IE257_RS37515_PKS_ER.1"
                     /locus_tag="IE257_RS37515"
                     /protein_end="1891"
                     /protein_start="1585"
                     /score="355.6"
                     /tool="antismash"
                     /translation="GTIENLALVPCPEVLDPLGPGQVRIAVHAVGMNFRDVVVALGVVT
                     TQKGIGGDVAGVVMETGSGVTNVAVGDRVLGLCSDSFGPVAVCDHRFLTPMPDGWSYEQ
                     AATIPITHLTAYYGLVDLAAVQQGESVLVHAAAGGVGIAAVQLARHLGAEVYGTASPGK
                     WQTLRDHGLDAPHIANSRTLEFEQWFMETSAGCGVDVVLDCLAGEFVDAGLRLQPRGGR
                     FLEMGKTDKRDPAQVAEAYPGVAYQAYDLMEAGPDRIQEMLVAVMDLYRAGALVPPPIT
                     TYDVRRARDAMRDLSQAKLVGKAVL"
     aSModule        complement(64..5106)
                     /domains="nrpspksdomains_IE257_RS37515_PKS_KS.1"
                     /domains="nrpspksdomains_IE257_RS37515_PKS_AT.1"
                     /domains="nrpspksdomains_IE257_RS37515_PKS_DH.1"
                     /domains="nrpspksdomains_IE257_RS37515_PKS_ER.1"
                     /incomplete
                     /locus_tags="IE257_RS37515"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(538..600)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0013"
                     /evalue="1.90E-09"
                     /label="PKSI-ER_m5"
                     /locus_tag="IE257_RS37515"
                     /protein_end="1733"
                     /protein_start="1712"
                     /score="29.7"
                     /tool="antismash"
                     /translation="GESVLVHAAAGGVGIAAVQLA"
     CDS_motif       complement(625..669)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0012"
                     /evalue="7.10E-05"
                     /label="PKSI-ER_m4"
                     /locus_tag="IE257_RS37515"
                     /protein_end="1704"
                     /protein_start="1689"
                     /score="16.1"
                     /tool="antismash"
                     /translation="AATIPITHLTAYYGL"
     CDS_motif       complement(856..891)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0011"
                     /evalue="3.20E-03"
                     /label="PKSI-ER_m1"
                     /locus_tag="IE257_RS37515"
                     /protein_end="1627"
                     /protein_start="1615"
                     /score="10.6"
                     /tool="antismash"
                     /translation="GMNFRDVVVALG"
     CDS_motif       complement(1927..1959)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0010"
                     /evalue="6.40E-03"
                     /label="PKSI-DH_m3"
                     /locus_tag="IE257_RS37515"
                     /protein_end="1270"
                     /protein_start="1259"
                     /score="9.5"
                     /tool="antismash"
                     /translation="GYHYGPAFRGL"
     aSDomain        complement(1927..2412)
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37515_PKS_DH.1"
                     /evalue="5.30E-58"
                     /label="IE257_RS37515_PKS_DH.1"
                     /locus_tag="IE257_RS37515"
                     /protein_end="1270"
                     /protein_start="1108"
                     /score="188.0"
                     /tool="antismash"
                     /translation="HPLLGAAVVMAGGEGVVLTGLLSGRTHPWLLDHAVSGTVLLPGTA
                     FVDLAIRAGDHLGHPHLEELTLQAPLLLGTGPDDDVTVQVRATPDAGADGCTVTVHSRT
                     GDGDWTLHATGTLGQEAPAEPVPDAAWPPAGAEPVALDGVYDELAEGGYHYGPAFRGL"
     CDS_motif       complement(2254..2328)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0009"
                     /evalue="8.30E-09"
                     /label="PKSI-DH_m2"
                     /locus_tag="IE257_RS37515"
                     /protein_end="1161"
                     /protein_start="1136"
                     /score="27.9"
                     /tool="antismash"
                     /translation="WLLDHAVSGTVLLPGTAFVDLAIRA"
     aSDomain        complement(2614..3504)
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37515_PKS_AT.1"
                     /evalue="4.60E-108"
                     /label="IE257_RS37515_PKS_AT.1"
                     /locus_tag="IE257_RS37515"
                     /protein_end="1041"
                     /protein_start="744"
                     /score="353.0"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFPGQGAQWAGMAAELLDSSPVFRARVQECEEALSPYVDWSLLAV
                     LRQEPGTPPWDRVDVVQPALWAVMVSLAEVWRSYGVEPDAVIGHSQGEIAAACVCGALD
                     LGDGARIVALRAKAIGSALAGRGGMVSVALPSAAARDLIAGWPGTISVASVNGPVSTVV
                     AGEPGALDELLARCAEDGVRARRIPVDYASHTAQVEEIEADLAAELKGIAPRSSSIPFF
                     STLTGGTLDTAELDAGYWYRNLRHTVEFEGATRAALAQGFTVFAEISPHPVLVPSVQDS
                     IDDAGAAAAAVGSLRR"
     CDS_motif       complement(2755..2802)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0008"
                     /evalue="2.30E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="IE257_RS37515"
                     /protein_end="994"
                     /protein_start="978"
                     /score="16.5"
                     /tool="antismash"
                     /translation="LDAGYWYRNLRHTVEF"
     CDS_motif       complement(2908..2940)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0007"
                     /evalue="5.10E-04"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="IE257_RS37515"
                     /protein_end="943"
                     /protein_start="932"
                     /score="12.8"
                     /tool="antismash"
                     /translation="VDYASHTAQVE"
     CDS_motif       complement(3157..3267)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0006"
                     /evalue="1.40E-17"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="IE257_RS37515"
                     /protein_end="860"
                     /protein_start="823"
                     /score="55.5"
                     /tool="antismash"
                     /translation="GVEPDAVIGHSQGEIAAACVCGALDLGDGARIVALRA"
     CDS_motif       complement(3271..3339)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0005"
                     /evalue="4.30E-09"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE257_RS37515"
                     /protein_end="822"
                     /protein_start="799"
                     /score="28.6"
                     /tool="antismash"
                     /translation="DRVDVVQPALWAVMVSLAEVWRS"
     CDS_motif       complement(3454..3510)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0004"
                     /evalue="1.40E-09"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE257_RS37515"
                     /protein_end="761"
                     /protein_start="742"
                     /score="29.5"
                     /tool="antismash"
                     /translation="VFVFPGQGAQWAGMAAELL"
     misc_feature    complement(3750..3752)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        complement(3832..5106)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37515_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="5.80E-185"
                     /label="IE257_RS37515_PKS_KS.1"
                     /locus_tag="IE257_RS37515"
                     /protein_end="635"
                     /protein_start="210"
                     /score="607.1"
                     /tool="antismash"
                     /translation="IAIVGMACRYPGGVSSPEELWELVLSGRDAIAGMPEDRGWDVERL
                     YDPELTRPGTSYVRQGGFLYDAADFDPEFFGISPREALAMDPQQRLLLETAWEALEHAG
                     IDPHAVRGSRTGVFTGMMYHDYATDRAELPEEAEGFIGTGSAGSVASGRIAFTLGLEGP
                     ALTIDTACSSSLVALHLAAQALRSGECDLALAGGVTVMSTPAVFVELSRQGGLSPDGRC
                     RSFADGADGTGWGEGAGLLLVERLSDARRNGHPVLAIVRGTAVNQDGASNGLTAPNGPA
                     QQRVIEEALDNAGLGLGDVDVVEAHGTGTTLGDPIEAQALLATYGRDRPAGRPLRLGSV
                     KSNMGHTQAAAGAAGIIKMVMAMRHGVMPKTLHVDSPSSQIDWTSGGVELLTEAQEWPA
                     DGAPRRAGVSSFGISGTNAHVIIEQP"
     CDS_motif       complement(4051..4095)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0003"
                     /evalue="1.40E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE257_RS37515"
                     /protein_end="562"
                     /protein_start="547"
                     /score="11.2"
                     /tool="antismash"
                     /translation="GSVKSNMGHTQAAAG"
     CDS_motif       complement(4156..4200)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0002"
                     /evalue="4.20E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE257_RS37515"
                     /protein_end="527"
                     /protein_start="512"
                     /score="12.6"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       complement(4576..4623)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37515_0001"
                     /evalue="1.20E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE257_RS37515"
                     /protein_end="387"
                     /protein_start="371"
                     /score="20.4"
                     /tool="antismash"
                     /translation="GPALTIDTACSSSLVA"
     aSDomain        complement(5173..5430)
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37515_PKS_PP.1"
                     /evalue="8.00E-30"
                     /label="IE257_RS37515_PKS_PP.1"
                     /locus_tag="IE257_RS37515"
                     /protein_end="188"
                     /protein_start="102"
                     /score="95.0"
                     /tool="antismash"
                     /translation="LAALPQAERTRRLLDLVRTHAATALGYPTSEGLDAKRTFRELGFD
                     SLAAIELRNGVGGATGLRLPATLVFDHPTPSAVVDFLLGEL"
ORIGIN
        1 cgccgagggt gccggtgccg ccggtgatca gcgccgtccc ctccgggtcg atcccctgcg
       61 ggacggtcag gacggccttg ccgaccagct tcgcctgact caggtcgcgc atcgcgtcac
      121 gggcacggcg cacgtcgtac gtggtgatgg gcgggggcac cagggcaccg gcccggtaca
      181 ggtccatcac cgcgacgagc atctcctgga tgcggtccgg tcccgcttcc atcaggtcgt
      241 aggcctggta ggcgacgccc gggtaggcct cggcgacctg cgcggggtca cgcttgtcgg
      301 tcttgcccat ttcgaggaag cgtccgccgc gcggctgcag tcgcaggccc gcgtccacga
      361 actcgcccgc caggcagtcc aggaccacgt ccacaccaca gccggcgctg gtctccatga
      421 accactgctc gaactccagg gtccgggagt tggcgatgtg cggtgcgtcc agaccgtgat
      481 cgcgcagggt ctgccacttg ccggggctcg ccgtgccgta cacctccgca ccgaggtgcc
      541 gggccagctg caccgcggcg atgccgacac cgccggccgc cgcgtgcacc aggaccgact
      601 cgccctgctg caccgcggcc aggtccacca gcccgtagta ggcggtgaga tgggtgatcg
      661 ggatcgtggc ggcctgctcg tacgaccatc cgtcgggcat cggggtcagg aaccggtggt
      721 cgcagacggc gaccggcccg aacgagtccg aacacaggcc cagcacccgg tcgccgacgg
      781 cgacgttcgt caccccggag ccggtctcca tcacgacgcc ggcgacatcg ccaccgatgc
      841 ccttctgggt ggtgaccacg ccgagcgcga ccacgacgtc acggaagttc atgccgaccg
      901 cgtgcaccgc gatgcggacc tggccggggc cgagggggtc gaggacctcc gggcagggga
      961 ccagcgccag gttctcgatg gtgccgctgc cggtgctttc caggcgccac ggcacccctc
     1021 cggacggcgg cagcagcgcc gagtgcgagc gggcgcgcgc cagccggggc gcggtgaccg
     1081 tgccgcggcg gacggcgatc cggggctcgg aaccctccag cgcggccggg aacgcgtccc
     1141 gggactccgg ccgaccgtcc aggtcgacga ggccgaagcg gtccgggttc tcggtctgcg
     1201 ccgaggtcac cagaccccag ctcgtcgcgt ggggcagatc ggtgacgtcc tcggcgtcgc
     1261 ccgcggcgac cgcccccgtc gtcaccagca ccaggcggga cgtggcgaag cgctcgtccg
     1321 ccagccaggc acggacccgc tccagggtct cctgcgcggc ggcgtgcgcc gcggtcaccg
     1381 gatcgccgga ccccgccggg gccaggcagg tgaccacggt gtccgggacc gggccgtccg
     1441 cggcgagcgc ggccaggtcc ggcaggacac gttccgcccc gtcggccagc ccgaacgggt
     1501 cctcgccgac cagcgtgtac gggaccgcgc cgtcgccctc cgcgccggcg ggcagcacgg
     1561 cccagtcgag gtggagcagc gcctcctcca gcgcgccgcc cgacgtctgc ccgatggcgg
     1621 ccggccgggt cacgagtgag cgcaccgcga cgacgggcct gcccgtgtcg tcggtgagca
     1681 gcagcgagag ctggtcaccg gccggcgtga gacgcacccg ggccgcagtg gccccggtcg
     1741 cgtacaggtg cacaccggtc caggcgaacg gcagcttcac gccggactcc tggtcaccgg
     1801 cgaccgccat ggcgtgcagc gcggagtcca gcagcgccgg gtggacgccg aaccgggcgg
     1861 cctcctcgcg ttccgtctcg ggcagccgga tctcggcgaa cagctcgccg tcccggcgcc
     1921 acaccgcttc cagtccccgg aacgccgggc cgtagtggta gccgccctcg gcgagttcgt
     1981 cgtagacgcc gtccagcgcg acgggctccg ccccggccgg cggccaggcc gcgtccggta
     2041 cgggctccgc gggcgcctcc tggcccaggg tgccggtcgc gtgcagcgtc cagtccccgt
     2101 cgccggtacg ggagtgcacg gtgaccgtgc agccgtccgc accggcgtcc ggggtggcac
     2161 ggacctgcac cgtgacgtcg tcgtcggggc cggtgcccag caggagcggt gcctgcaggg
     2221 tgagttcctc caggtgcggg tggcccaggt ggtcgcccgc ccggatcgcc agatccacga
     2281 aggccgtgcc gggcagcagg accgtgccgg agacggcgtg gtcgaggagc cacggatggg
     2341 tgcgcccgga cagcaggccg gtgagcacaa cgccctcacc gcccgccatg accacggccg
     2401 cgcccagcaa cggatggtcc gccgactcca ggccggtcgc cgtcaggtct ccggctccgc
     2461 gccgcccctc cagccagaac cgccggcgct ggaaggcgta cgtcggcagc tccacctgcc
     2521 gggcggcgcc ggcgaacacc ggcgtccagt cgatgtcgac gcccagcacc ccggcctcgg
     2581 ccaccgaggt cagcatgcga cgcagaccgc cgtcctcgcg gcgcagcgag cccacggccg
     2641 ccgctgccgc gccggcatcg tcgatgctgt cctggacgga cggcaccagc accggatgcg
     2701 ggctgatctc cgcgaagacg gtgaagccct gcgccagcgc cgcacgggtg gctccctcga
     2761 actccacggt gtggcgcagg ttccggtacc agtacccggc gtccagctcc gcggtgtcca
     2821 gcgtgccccc ggtcagggtg gagaagaacg ggatcgagga cgagcgcggt gcgatgccct
     2881 tcagctccgc ggcgagatcg gcctcgatct cctccacctg agccgtgtgg gaggcgtagt
     2941 ccaccggaat gcggcgggcc cggacaccgt cctcggcgca gcgggcgagc agctcgtcga
     3001 gcgcaccggg ttcaccggcg accaccgtgc tgaccggtcc gttgaccgac gccaccgaaa
     3061 tggttcccgg ccaacccgcg atcaggtccc gcgccgccgc ggagggcagt gccaccgaca
     3121 ccatgccgcc ccgtccggcc agcgcggagc cgatcgcctt ggcccgcagc gccacgatcc
     3181 gggccccgtc tccgaggtcc agtgccccgc agacgcacgc ggcggcgatc tccccctggg
     3241 agtggccgat caccgcgtcg ggctcgaccc cgtaggagcg ccacacctcc gccagcgaga
     3301 ccatcacggc ccacagcgcg ggctgcacca cgtcgacccg gtcccagggc ggggtgcccg
     3361 gctcctgccg cagcacggcg agcagcgacc agtcgacgta cgggctgagc gcctcctcgc
     3421 actcctggac gcgggcccgg aagaccggcg aggagtccag cagctcggcc gccatccccg
     3481 cccactgcgc gccctgtccg gggaagacga acaccacccg gtcgtcgccg agtacggaac
     3541 cctcggccac gcccggcggc agcgccgcgt ccggatcgcc gacccgaccc gccagcccgg
     3601 acgcaccgga cagcagcgcg tcccggtcgg cgcccagcac caccgcacgg tgggggagcg
     3661 ccgcccggcc ggtcagtgtc cggcccacgt ccgccggcga cagctccggg tgcgcctcga
     3721 ggtgcgccac cagctgctcg gcctgcgcgt aaagcgctgc ccggtcgtgc ccggagagca
     3781 cccacggaac cacctcgggg gccgcgggca cagccgccac cggcgcgggg gccggaggct
     3841 gctcgatgat gacgtgcgcg ttggtgccgc tgatcccgaa ggaggacaca ccggcccggc
     3901 gcggagcgcc gtccgccggc cactcctgcg cctcggtcag cagctccacc ccgccggagg
     3961 tccagtcgat ctgcgaactg ggcgagtcca cgtgcagggt cttcggcatg acgccgtgcc
     4021 gcatcgccat gaccatcttg atgatgcccg cggcacccgc cgcggcctgg gtgtggccca
     4081 tgttggactt gaccgatccc agccgcagcg gccgtccggc ggggcggtcc cggccgtagg
     4141 tggccagcag cgcctgcgcc tcgatggggt cgccgagcgt ggtgcccgtg ccgtgcgcct
     4201 ccaccacgtc gacgtcgccc aggccgaggc ccgcgttgtc cagggcctct tcgatcacgc
     4261 gctgctgcgc gggtccgttc ggcgcggtca gaccgttgga cgcaccgtcc tggttgacgg
     4321 cggttccgcg cacgatcgcc agtacgggat ggccgttgcg gcgggcgtcg gagagccgct
     4381 cgaccagcag gagtccggca ccctcgcccc agccggtgcc gtcggcaccg tcggcgaagg
     4441 agcggcaccg gccgtcgggg gacaggccgc cctgccggga cagctccacg aacacggcgg
     4501 gggtcgacat cacggtcacg ccgccggcca gcgccaggtc gcactcaccg ctgcgcagtg
     4561 cctgcgcggc caggtgcagg gccacgagcg aggacgagca cgcggtgtcg atggtcagcg
     4621 cggggccttc cagccccagg gtgaacgcga tccgcccgga ggcgacggag ccggccgaac
     4681 cggttcctat gaacccctcg gcctcctccg gcagttcggc ccggtcggtg gcgtagtcgt
     4741 ggtacatcat gccggtgaac acgccggtcc ggctgccgcg caccgcgtgc gggtcgatgc
     4801 ccgcgtgctc cagcgcctcc caggcggtct ccagcagcag ccgctgctgc gggtccatgg
     4861 ccagggcctc gcgcggcgag atgccgaaga actcggggtc gaagtcggcc gcatcgtaga
     4921 gaaatccgcc ctggcgcacg tacgaggtgc ccggccgggt cagctcgggg tcgtacagcc
     4981 gctccacgtc ccagccgcgg tcctcgggca tgccggcgat cgcgtcccgg ccggagagca
     5041 ccagctccca cagttcctcg ggagacgaca ccccgcccgg gtagcggcag gccatgccca
     5101 cgatcgcgat cggctcgtcc gccccggagg cggacacggc cggccgcgcg gcgggccgcg
     5161 cctgcacccc gcccgtcagc tcgccgagca ggaagtccac cacggcggac ggcgtcgggt
     5221 ggtcgaagac gagggtcgcg ggcagccgca ggccggtcgc gccgcccacg ccgttgcgca
     5281 gctcgatcgc cgccagcgag tcgaagccca gctcacggaa cgtccgcttc gcatccagtc
     5341 cctcggacgt cgggtagccc agcgcggtcg ccgcgtgcgt ccggacgagg tccagcagcc
     5401 tccgcgtccg ctcggcctgc ggcagcgccg ccagctcggc cgcgaaggcc ggactcccgg
     5461 cggttcccgg ggcggccgcc gcccggcggg ccggagcccg caccaggccg cgcagcaacg
     5521 gcggcagcgt gcccgcaccg gcctgggtcc gcagtgccgc gaggtccagg ggcatcggca
     5581 cgcagaccgg cgcgtccagc gcgacggccg cgtcgaacag tgcgaggccc tgctcctcgg
     5641 agatgggcag caccccggcg ccggccatgc ggcgcatcac cgcttcctcg tcggccaggt
     5701 ggccggtcat gccgctgcgt tgggcccaga atccccaggc ga
//
