LOCUS       NZ_BMVL01000039         5948 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence39, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000039
VERSION     NZ_BMVL01000039.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5948
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..5905)
                     /locus_tag="IE257_RS37510"
     CDS             complement(<1..5905)
                     /NRPS_PKS="Domain: AMP-binding (25-287). E-value: 3.2e-66.
                     Score: 215.5. Matches aSDomain:
                     nrpspksdomains_IE257_RS37510_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (398-465). E-value: 8.1e-27. Score:
                     85.2. Matches aSDomain: nrpspksdomains_IE257_RS37510_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (481-781). E-value:
                     2.4e-115. Score: 376.6. Matches aSDomain:
                     nrpspksdomains_IE257_RS37510_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (951-1341). E-value:
                     7.4e-104. Score: 339.5. Matches aSDomain:
                     nrpspksdomains_IE257_RS37510_AMP-binding.2"
                     /NRPS_PKS="Domain: PCP (1448-1515). E-value: 1.3e-27.
                     Score: 87.7. Matches aSDomain:
                     nrpspksdomains_IE257_RS37510_PCP.2"
                     /NRPS_PKS="Domain: Condensation_LCL (1530-1831). E-value:
                     6.5e-110. Score: 358.8. Matches aSDomain:
                     nrpspksdomains_IE257_RS37510_Condensation_LCL.2"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982969.1"
                     /locus_tag="IE257_RS37510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_189974582.1"
                     /sec_met_domain="Condensation (E-value: 5.4e-71, bitscore:
                     225.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.4e-110, bitscore:
                     355.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 9.3e-20, bitscore:
                     57.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVALDSTLMGVQLSMLEDTAPEVSVSADGLAYVIYTSGSTGRPKG
                     VAVTHGGLANYAQWAAGSYGAGGGAPLHSSLAFDLTVTSVVVPLISGAPVVVSREGGAE
                     GLAELLRDGGGFGLTKAVPAHLPLLSEMLPDGEISGAASTWVVGGEALSGSVVQAWLER
                     APGSVVVNEYGPTETVVGCAVFEVRAGDEIGDVVPIGRPVANTQLYVLDGFLRPVAPGV
                     AGELYIAGAQLARGYVRRPELTAERFVASPFERGGRMYRSGDLARWRVDGQLEFLGRAD
                     EQVKVRGFRIEPGEVEGAIASHPQVGQVAVVAREDVPGDVRLVAYVVADDPDEDTDDLS
                     HSVAEAVARRLPEYMVPSAVVVLEALPLTGNGKLDRRALPAPEVAGGAGSGRAPANERE
                     RVLCQAFAEVLRVPEVGVDDDFFLLGGHSLLAVRLVSRIRVALDVDVEIRAVFEAPTPA
                     ALAALLPDAEGARTALTAMERPELVPLSFAQRRLWFINQLEGPSASYNIPVALRLTGDV
                     DRGALNAALRDVLGRHEVLRTVFATVDGEPYQRIISLDELDWELEVVEVAAAALQDAVS
                     AAFGHAFDLAEQVPFRSWLFRGGEGDHVLVVVMHHIAGDGWSREPLARDLSVAYAARRD
                     GRAPEWEPLPVQYADYSLWQRELLGDGDEPDSLMAAQIAFWRDALGDIPEELELPVDRT
                     RPPTASYNGLGVPLEIPADVHARLAEMARAEGVTMFMVLQSALAVLLNRLGAGTDIPIG
                     TANAGRSDEALDDLVGFFINTLVLRTDLSGDPTFADVLARVRETGLSAFAHQDVPFEKL
                     VEELAPARSMARHPLFQVSLSLENTREAVLDLSGLGAGELSAGETVSRFDLEVSVREGF
                     DASGAPAGLYGAVVAAADLFELESVERIGARLGRVLTAVAADRQLPLSALDVLDGEERH
                     RLLSEWNDTAAEVEPSTLPALFAAQAVRTPSAPALVADGVSLSYAELDARANRLARLLA
                     GRGVGPESVVAVLLDRGVDLVVTLLAVVKAGGAYLPIDPAAPAERIALMLGDAGVSLVV
                     SSSAVAGAVSAGLPVIVSDAPSVAEELAGLAEEPFGAEVLPSHAAYVIFTSGSTGRPKG
                     VLVPHEGVVNLLASGGWGAGAGSRVLQFASVGFDAAVWEVWGALSSGGCLVVAPADELL
                     PGAGLAGVVERFGVTDVLLPPSVLGVLEDGTLGSVRTLLSGGEALGAELVDRWGAGRRL
                     VNAYGPTEASVCVATAGPLSAGDEVSVGRPNVNTRVFVLDGHLMPVPVGVTGELYVAGA
                     QLARGYAGRAAMTAERFVADPFAADGTRMYRTGDRVRWTADGRLVFVGRVDEQVKIRGF
                     RIEPGEVQTVVAAHPRVAQAVVVVREDAPGDKRLVAYVSATAEYSAELPEEIRTYAAGL
                     LPEYMVPAAVVVLDALPLTTSGKLDRKALPAPAYTAGAGGAPASPQEELLCSAFAEVLG
                     LATVGVDDDFFALGGHSLLAVRLVSRVRSVLGVEVPLRTLFEAPTVARLAARLTGADRA
                     RVALRAAVRPERVPLSFAQRRLWFLNQLEGPGATYNVPVTLRLPGVVDRDVLGAAFRDV
                     LGRHEVLRTVFPVVDGEPYQRIVGIDDLDWELSVSEVASADLADAIAAASEYAFDLSAE
                     PPVKAWLFEAGPDERALVVVMHHVASDGWSEGPLARDLSVAYAARSEGRAPEWDALPVQ
                     YADYALWQHELLGDEQDPDSVIFRQIAYWRDALSGSPEELELPFDRPRPAVGSHRGHRV
                     SLEVPAVVHARLVEVARVEGVTPFMVLQASLALLLSKLGAGVDIPIGSANAGRTDEALD
                     DLIGFFINTLVVRTDLSGDPTFREVLGRVRERTLSALAHQDVPFEKLVEELSPARSMAR
                     HPLFQVVLTTQNTIEAVLDLPGLRADSPADGSENGETAEATDATEVYESAAKFDLDVIV
                     AEEYDAAGRPAGVSGSVTVSADLFDPVWAGRIASA"
     source          1..5948
                     /culture_collection="JCM:4726"
                     /db_xref="taxon:1895"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces avidinii"
                     /strain="JCM 4726"
                     /submitter_seqid="sequence39"
                     /type_material="type strain of Streptomyces avidinii"
     region          1..5948
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..5948
                     /SMILES="NC(CC(=O)N)C(=O)NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5948
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:5905](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..5905)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(260..373)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0025"
                     /evalue="2.50E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1882"
                     /protein_start="1844"
                     /score="64.9"
                     /tool="antismash"
                     /translation="RERTLSALAHQDVPFEKLVEELSPARSMARHPLFQVVL"
     aSDomain        complement(413..1315)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37510_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="6.50E-110"
                     /label="IE257_RS37510_Condensation_LCL.2"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1831"
                     /protein_start="1530"
                     /score="358.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHVASDG"
                     /tool="antismash"
                     /translation="ERVPLSFAQRRLWFLNQLEGPGATYNVPVTLRLPGVVDRDVLGAA
                     FRDVLGRHEVLRTVFPVVDGEPYQRIVGIDDLDWELSVSEVASADLADAIAAASEYAFD
                     LSAEPPVKAWLFEAGPDERALVVVMHHVASDGWSEGPLARDLSVAYAARSEGRAPEWDA
                     LPVQYADYALWQHELLGDEQDPDSVIFRQIAYWRDALSGSPEELELPFDRPRPAVGSHR
                     GHRVSLEVPAVVHARLVEVARVEGVTPFMVLQASLALLLSKLGAGVDIPIGSANAGRTD
                     EALDDLIGFFINTLVVRTDL"
     CDS_motif       complement(422..511)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0024"
                     /evalue="2.80E-12"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1828"
                     /protein_start="1798"
                     /score="39.0"
                     /tool="antismash"
                     /translation="DIPIGSANAGRTDEALDDLIGFFINTLVVR"
     CDS_motif       complement(779..817)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0023"
                     /evalue="1.20E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1709"
                     /protein_start="1696"
                     /score="17.7"
                     /tool="antismash"
                     /translation="QYADYALWQHELL"
     CDS_motif       complement(866..931)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0022"
                     /evalue="8.40E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1680"
                     /protein_start="1658"
                     /score="24.8"
                     /tool="antismash"
                     /translation="MHHVASDGWSEGPLARDLSVAY"
     CDS_motif       complement(1133..1249)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0021"
                     /evalue="2.90E-15"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1591"
                     /protein_start="1552"
                     /score="48.6"
                     /tool="antismash"
                     /translation="ATYNVPVTLRLPGVVDRDVLGAAFRDVLGRHEVLRTVFP"
     CDS_motif       complement(1268..1300)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0020"
                     /evalue="7.50E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1546"
                     /protein_start="1535"
                     /score="9.1"
                     /tool="antismash"
                     /translation="SFAQRRLWFLN"
     aSDomain        complement(1361..1561)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37510_PCP.2"
                     /evalue="1.30E-27"
                     /label="IE257_RS37510_PCP.2"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1515"
                     /protein_start="1448"
                     /score="87.7"
                     /tool="antismash"
                     /translation="EELLCSAFAEVLGLATVGVDDDFFALGGHSLLAVRLVSRVRSVLG
                     VEVPLRTLFEAPTVARLAARLT"
     aSModule        complement(1361..4462)
                     /complete
                     /domains="nrpspksdomains_IE257_RS37510_Condensation_LCL.1"
                     /domains="nrpspksdomains_IE257_RS37510_AMP-binding.2"
                     /domains="nrpspksdomains_IE257_RS37510_PCP.2"
                     /locus_tags="IE257_RS37510"
                     /monomer_pairings="Leu -> Leu"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1523..1555)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0019"
                     /evalue="1.80E-02"
                     /label="Cy4"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1461"
                     /protein_start="1450"
                     /score="8.3"
                     /tool="antismash"
                     /translation="LLCSAFAEVLG"
     CDS_motif       complement(1610..1636)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0018"
                     /evalue="1.50E-01"
                     /label="NRPS-A_a10"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1432"
                     /protein_start="1423"
                     /score="5.2"
                     /tool="antismash"
                     /translation="GKLDRKALP"
     CDS_motif       complement(1856..1918)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0017"
                     /evalue="5.20E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1350"
                     /protein_start="1329"
                     /score="34.5"
                     /tool="antismash"
                     /translation="FVGRVDEQVKIRGFRIEPGEV"
     aSDomain        complement(1883..3052)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37510_AMP-binding.2"
                     /evalue="7.40E-104"
                     /label="IE257_RS37510_AMP-binding.2"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1341"
                     /protein_start="951"
                     /score="339.5"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="FAAQAVRTPSAPALVADGVSLSYAELDARANRLARLLAGRGVGPE
                     SVVAVLLDRGVDLVVTLLAVVKAGGAYLPIDPAAPAERIALMLGDAGVSLVVSSSAVAG
                     AVSAGLPVIVSDAPSVAEELAGLAEEPFGAEVLPSHAAYVIFTSGSTGRPKGVLVPHEG
                     VVNLLASGGWGAGAGSRVLQFASVGFDAAVWEVWGALSSGGCLVVAPADELLPGAGLAG
                     VVERFGVTDVLLPPSVLGVLEDGTLGSVRTLLSGGEALGAELVDRWGAGRRLVNAYGPT
                     EASVCVATAGPLSAGDEVSVGRPNVNTRVFVLDGHLMPVPVGVTGELYVAGAQLARGYA
                     GRAAMTAERFVADPFAADGTRMYRTGDRVRWTADGRLVFVGRVDEQVKIR"
     CDS_motif       complement(2003..2092)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0016"
                     /evalue="9.30E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1301"
                     /protein_start="1271"
                     /score="46.7"
                     /tool="antismash"
                     /translation="PVGVTGELYVAGAQLARGYAGRAAMTAERF"
     CDS_motif       complement(2195..2221)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0015"
                     /evalue="5.90E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1237"
                     /protein_start="1228"
                     /score="6.6"
                     /tool="antismash"
                     /translation="YGPTEASVC"
     CDS_motif       complement(2570..2629)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0014"
                     /evalue="3.20E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1112"
                     /protein_start="1092"
                     /score="31.9"
                     /tool="antismash"
                     /translation="AYVIFTSGSTGRPKGVLVPH"
     CDS_motif       complement(2825..2866)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0013"
                     /evalue="8.80E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="IE257_RS37510"
                     /protein_end="1027"
                     /protein_start="1013"
                     /score="15.8"
                     /tool="antismash"
                     /translation="LAVVKAGGAYLPID"
     CDS_motif       complement(3404..3523)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0012"
                     /evalue="3.40E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE257_RS37510"
                     /protein_end="834"
                     /protein_start="794"
                     /score="64.4"
                     /tool="antismash"
                     /translation="RETGLSAFAHQDVPFEKLVEELAPARSMARHPLFQVSLSL"
     aSDomain        complement(3563..4462)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37510_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.40E-115"
                     /label="IE257_RS37510_Condensation_LCL.1"
                     /locus_tag="IE257_RS37510"
                     /protein_end="781"
                     /protein_start="481"
                     /score="376.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAGDG"
                     /tool="antismash"
                     /translation="LVPLSFAQRRLWFINQLEGPSASYNIPVALRLTGDVDRGALNAAL
                     RDVLGRHEVLRTVFATVDGEPYQRIISLDELDWELEVVEVAAAALQDAVSAAFGHAFDL
                     AEQVPFRSWLFRGGEGDHVLVVVMHHIAGDGWSREPLARDLSVAYAARRDGRAPEWEPL
                     PVQYADYSLWQRELLGDGDEPDSLMAAQIAFWRDALGDIPEELELPVDRTRPPTASYNG
                     LGVPLEIPADVHARLAEMARAEGVTMFMVLQSALAVLLNRLGAGTDIPIGTANAGRSDE
                     ALDDLVGFFINTLVLRTDL"
     CDS_motif       complement(3572..3661)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0011"
                     /evalue="3.00E-12"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE257_RS37510"
                     /protein_end="778"
                     /protein_start="748"
                     /score="38.9"
                     /tool="antismash"
                     /translation="DIPIGTANAGRSDEALDDLVGFFINTLVLR"
     CDS_motif       complement(3929..3967)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0010"
                     /evalue="1.20E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE257_RS37510"
                     /protein_end="659"
                     /protein_start="646"
                     /score="17.8"
                     /tool="antismash"
                     /translation="QYADYSLWQRELL"
     CDS_motif       complement(4016..4081)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0009"
                     /evalue="1.10E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE257_RS37510"
                     /protein_end="630"
                     /protein_start="608"
                     /score="27.5"
                     /tool="antismash"
                     /translation="MHHIAGDGWSREPLARDLSVAY"
     CDS_motif       complement(4283..4399)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0008"
                     /evalue="5.70E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE257_RS37510"
                     /protein_end="541"
                     /protein_start="502"
                     /score="57.3"
                     /tool="antismash"
                     /translation="ASYNIPVALRLTGDVDRGALNAALRDVLGRHEVLRTVFA"
     CDS_motif       complement(4421..4450)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0007"
                     /evalue="1.20E-02"
                     /label="C1_LCL_004-017"
                     /locus_tag="IE257_RS37510"
                     /protein_end="495"
                     /protein_start="485"
                     /score="8.5"
                     /tool="antismash"
                     /translation="SFAQRRLWFI"
     aSDomain        complement(4511..4711)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37510_PCP.1"
                     /evalue="8.10E-27"
                     /label="IE257_RS37510_PCP.1"
                     /locus_tag="IE257_RS37510"
                     /protein_end="465"
                     /protein_start="398"
                     /score="85.2"
                     /tool="antismash"
                     /translation="ERVLCQAFAEVLRVPEVGVDDDFFLLGGHSLLAVRLVSRIRVALD
                     VDVEIRAVFEAPTPAALAALLP"
     aSModule        complement(4511..5830)
                     /complete
                     /domains="nrpspksdomains_IE257_RS37510_AMP-binding.1"
                     /domains="nrpspksdomains_IE257_RS37510_PCP.1"
                     /locus_tags="IE257_RS37510"
                     /monomer_pairings="Asn -> Asn"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(4673..4705)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0006"
                     /evalue="4.70E-01"
                     /label="Cy4"
                     /locus_tag="IE257_RS37510"
                     /protein_end="411"
                     /protein_start="400"
                     /score="3.8"
                     /tool="antismash"
                     /translation="VLCQAFAEVLR"
     CDS_motif       complement(4766..4792)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0005"
                     /evalue="1.50E-01"
                     /label="NRPS-A_a10"
                     /locus_tag="IE257_RS37510"
                     /protein_end="380"
                     /protein_start="371"
                     /score="5.2"
                     /tool="antismash"
                     /translation="GKLDRRALP"
     CDS_motif       complement(5015..5080)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0004"
                     /evalue="7.90E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="IE257_RS37510"
                     /protein_end="297"
                     /protein_start="275"
                     /score="37.1"
                     /tool="antismash"
                     /translation="FLGRADEQVKVRGFRIEPGEVE"
     aSDomain        complement(5045..5830)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37510_AMP-binding.1"
                     /evalue="3.20E-66"
                     /label="IE257_RS37510_AMP-binding.1"
                     /locus_tag="IE257_RS37510"
                     /protein_end="287"
                     /protein_start="25"
                     /score="215.5"
                     /specificity="substrate consensus: Asn"
                     /tool="antismash"
                     /translation="SADGLAYVIYTSGSTGRPKGVAVTHGGLANYAQWAAGSYGAGGGA
                     PLHSSLAFDLTVTSVVVPLISGAPVVVSREGGAEGLAELLRDGGGFGLTKAVPAHLPLL
                     SEMLPDGEISGAASTWVVGGEALSGSVVQAWLERAPGSVVVNEYGPTETVVGCAVFEVR
                     AGDEIGDVVPIGRPVANTQLYVLDGFLRPVAPGVAGELYIAGAQLARGYVRRPELTAER
                     FVASPFERGGRMYRSGDLARWRVDGQLEFLGRADEQVKVR"
     CDS_motif       complement(5162..5248)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0003"
                     /evalue="1.30E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="IE257_RS37510"
                     /protein_end="248"
                     /protein_start="219"
                     /score="52.6"
                     /tool="antismash"
                     /translation="PGVAGELYIAGAQLARGYVRRPELTAERF"
     CDS_motif       complement(5366..5392)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0002"
                     /evalue="3.40E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="IE257_RS37510"
                     /protein_end="180"
                     /protein_start="171"
                     /score="4.4"
                     /tool="antismash"
                     /translation="EYGPTETVV"
     CDS_motif       complement(5756..5815)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE257_RS37510_0001"
                     /evalue="1.20E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="IE257_RS37510"
                     /protein_end="50"
                     /protein_start="30"
                     /score="33.2"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVAVTH"
ORIGIN
        1 acgccgacgc gatccgcccg gcccacaccg gatcgaacaa atccgccgac accgtcacgg
       61 aaccggatac gcccgccggc cggccggccg cgtcgtactc ctcggcgacg atgacgtcca
      121 ggtcgaactt cgcggccgac tcgtacacct ccgtggcgtc ggtcgcctcc gcggtctcgc
      181 cgttctccga gccgtcggcc gggctgtcgg cccgcaggcc ggggaggtcg agcaccgcct
      241 cgatggtgtt ctgggtcgtc aggacgacct ggaagagggg gtgtcgggcc atcgagcggg
      301 ccggtgacag ttcctccacc agcttctcga acggcacgtc ctggtgcgcc agcgccgaca
      361 gcgtccgctc ccggacccgg cccagcacct cacggaacgt cggatcaccc gacagatccg
      421 tccgcaccac cagcgtgttg atgaagaacc cgatcaggtc gtccagcgcc tcgtccgtcc
      481 gcccggcgtt cgccgaaccg atcggaatgt ccacccccgc ccccagcttg gagagcagca
      541 gcgccagcga cgcctgcagc accatgaacg gggtcacacc ctcgacccgc gccacctcca
      601 ccaaccgcgc atgcacgacc gccggaacct ccagggagac ccggtgtccg cggtgcgagc
      661 cgacggccgg acgcggccgg tcgaacggca gctccagctc ctccggcgaa ccggacaatg
      721 catcgcgcca gtaggcgatc tgacggaaaa tcacactgtc ggggtcctgc tcgtcaccca
      781 gcagctcgtg ctgccagagg gcgtagtcgg cgtactggac cggcagcgcg tcccactcgg
      841 gcgcccggcc ctcgctccgc gccgcgtagg cgacggacag gtcccgcgcc agcgggcctt
      901 ccgaccagcc gtcactggcg acgtggtgca tcaccacgac cagggcccgc tcatccggac
      961 ccgcctcgaa cagccatgcc ttgacggggg gttcggcgga gaggtcgaag gcgtactccg
     1021 acgccgcggc gatcgcgtcc gcgaggtcgg ccgaggcgac ctcggagacc gacagctccc
     1081 agtccaggtc gtcgatgccg acgatccgct ggtacggctc gccgtcgacg acggggaaca
     1141 cggtgcggag cacctcgtgc cgcccgagca cgtcacggaa cgcagcgccc aggacgtcgc
     1201 ggtccaccac gcccggaagg cggagcgtca ccggcacgtt gtaggtcgcc ccgggccctt
     1261 cgagctggtt gaggaaccac aggcggcgct gcgcgaacga caacggcacc cgctccgggc
     1321 gcaccgcggc cctcagcgcg acccgcgccc ggtccgcacc cgtcagccgg gccgccaggc
     1381 gcgccaccgt cggggcctcg aacagcgtcc gcagcggtac ctcgacgccc aggacggacc
     1441 gcacccggct caccaggcgc accgccagga gcgagtggcc ccccagcgcg aagaagtcgt
     1501 cgtccacgcc gaccgtcgcc agccccagga cctcggcgaa tgccgagcac agcagttcct
     1561 cctggggaga cgccggtgcg ccgccggcgc cggcggtgta cgcgggggcg ggcagggcct
     1621 tgcggtccag cttcccgctc gtcgtcagcg gcagcgcgtc caggacgacg accgcggccg
     1681 ggaccatgta ttccggcagc aggccggccg cgtatgtccg gatctcctcc gggagttccg
     1741 cggagtactc agcggtcgcg gagacgtagg cgacgagccg cttgtcaccc ggcgcgtcct
     1801 cccggaccac cactacggcc tgcgcgacgc ggggatgggc ggcgacgacg gtctggacct
     1861 cgcccggttc gatccggaag ccacggatct tcacctgctc gtccacgcgg cccacgaaca
     1921 ccagccggcc gtcggccgtc caccgcaccc ggtcacccgt gcggtacatc cgcgtgccgt
     1981 ccgccgcgaa ggggtccgcg acgaaccgct cggcggtcat cgccgcacgg ccggcgtagc
     2041 cccgggccaa ctgcgcaccg gccacgtaca gctcgcccgt gacacccacg ggcaccggca
     2101 tgaggtggcc gtcgagcacg aacacccgcg tgttcacgtt cggacgaccc accgacacct
     2161 cgtcacccgc ggacagcgga ccggccgtcg ccacgcacac cgacgcttcc gtcggcccgt
     2221 acgcgttcac caggcggcgt cccgcgcccc agcggtcgac cagctcggca cccagcgcct
     2281 caccacccga aagcagcgtg cgtacggagc cgagggtgcc gtcctccagc acgcccagca
     2341 ccgacggggg cagcaggacg tccgtcacgc cgaaccgttc caccaccccg gccaggcccg
     2401 cacccggcag cagttcgtcg gccggtgcca ccacgaggca cccgcccgag gacagcgcgc
     2461 cccacacttc ccagaccgcc gcgtcgaagc ccaccgacgc gaactgcagc acgcgcgaac
     2521 ccgcacccgc accccagcca ccggacgcca gcaggttcac cacgccctcg tgcggcacca
     2581 gcacgccctt ggggcgtccc gtcgagcccg aggtgaagat cacgtacgcc gcgtgcgagg
     2641 gcagcacctc ggcaccgaac ggctcctccg cgaggcccgc cagctcctcg gccacggacg
     2701 gcgcgtccga gacgatcacg ggcagaccgg cggacaccgc acccgcgacg gccgacgacg
     2761 acaccacgag ggagacaccc gcgtcaccca gcatcaaggc gatccgctcc gccggggcgg
     2821 cgggatcgat cggcaggtag gccccgccgg ccttcacgac cgcaaggagc gtcaccacca
     2881 ggtcgacccc gcggtccagc agcaccgcga cgaccgactc cggaccgact ccgcgcccgg
     2941 ccagcaggcg cgccagcctg ttcgcccgcg cgtccagctc ggcgtacgac aggctcacgc
     3001 cgtcggcgac cagcgcgggt gcgctcggcg tccgtaccgc ctgcgccgcg aacagcgcgg
     3061 gcagcgtcga cggctcgacc tcggctgccg tgtcgttcca ctcgctcagc agacggtgac
     3121 gctcctcccc gtccagcacg tccagcgcac tcagcggcag ctgccggtcg gcggccaccg
     3181 cggtcagcac ccggccgagc cgcgccccga tccgctccac cgactcgagc tcgaacaggt
     3241 ccgccgccgc gacgaccgcg ccgtacagac ccgcaggtgc gccggaggcg tcgaagccct
     3301 cccggacgct gacttccagg tcgaaccggg acacggtctc cccggccgac agctcaccgg
     3361 cgcccagccc ggacaggtcg agcacggcct ccctggtgtt ctccagcgac agcgacacct
     3421 ggaacagcgg gtggcgggcc atcgagcggg ccggggccag ctcctcgacc agcttctcga
     3481 acggcacgtc ctggtgggcg aaggccgaca ggccggtctc gcgcacccgg gccaggacgt
     3541 cggcgaacgt ggggtcgccg gacaggtccg tccgcagtac gagtgtgttg atgaagaagc
     3601 cgaccaggtc gtcgagcgcc tcgtccgaac ggccggcgtt cgccgtgccg atcggaatgt
     3661 ccgtaccggc gccgagccgg ttcaacagga cggccagggc gctctgcagc accatgaaca
     3721 tggtcacgcc ctcggcacgc gccatctcgg cgaggcgggc gtgcacgtcc gccgggatct
     3781 ccagcgggac gccgagcccg ttgtaggaag cggtcggggg ccgcgtacgg tcgaccggca
     3841 gctccagctc ttcggggatg tcgccgagag cgtcacgcca gaaggcgatc tgggccgcca
     3901 tcagactgtc cggctcgtct ccgtcgccca ggagctcacg ctgccacagg gagtagtcgg
     3961 cgtactgcac cggcagcggc tcccactccg gagcccggcc gtcgcggcgt gcggcgtacg
     4021 cgaccgacag gtcccgtgcc agcggctcgc gcgaccagcc gtcgcccgcg atgtggtgca
     4081 tcaccacgac gagcacgtgg tcgccctcgc cgccgcggaa cagccagctg cggaagggaa
     4141 cctgctccgc caggtcgaag gcgtggccga acgcggcgga caccgcgtcc tgcagtgcgg
     4201 cggccgcgac ctccacgacc tccagctccc agtccagttc gtcgagactg atgatccgct
     4261 ggtacggctc gccgtccacc gtcgcgaaga ccgtccgcag cacctcgtgc cgccccagga
     4321 cgtcgcgcag cgcggcgttc agggccccgc ggtcgacgtc gccggtcagc cgcagcgcca
     4381 ccgggatgtt gtaggacgca ctcgggcctt ccagctggtt gatgaaccag aggcgccgct
     4441 gcgcgaagga gagcggtacc agctcggggc gctccatggc cgtgagtgcc gtacgggccc
     4501 cctcggcgtc cgggagcagg gcggcgaggg ccgccggtgt gggggcctcg aacacggcgc
     4561 ggatctccac gtcgacgtcg agcgcgaccc ggatccggct caccagtcgg acggcgagca
     4621 gggagtgacc gccgagcagg aagaagtcgt cgtcgacccc gacttcgggc acccggagga
     4681 cttccgcgaa ggcctggcac agcacccgct cgcgctcgtt cgccggggcc cggcccgacc
     4741 ccgcgccacc cgccacttcg ggggcgggca gagccctgcg gtccagcttc ccgttcccgg
     4801 tcaacggcag cgcctccagc accaccacag ccgagggaac catgtactcc ggcagccgcc
     4861 gggccacggc ctcggcgacg gagtgcgaga ggtcgtccgt gtcctcgtca gggtcgtcgg
     4921 caacgacgta ggcgaccaga cggacatcgc ccggcacgtc ctcacgcgcc accaccgcga
     4981 cctgacccac ctgcggatgc gacgcgatgg caccctcgac ctcgcccggc tcgatccgga
     5041 aaccacgcac cttgacctgc tcgtcagcac gccccaggaa ctccagctga ccatccacac
     5101 gccaacgagc caggtcaccc gagcggtaca tccggccacc acgctcgaac gggctcgcca
     5161 cgaaccgctc cgccgtcagc tccggacgcc ggacataacc acgggccagc tgagcacccg
     5221 cgatatacaa ctcacccgcc acacccggag caaccggccg cagaaaacca tccaggacat
     5281 aaagctgcgt gttcgcgacc ggacgaccga tcgggaccac gtcaccgatc tcatcaccgg
     5341 cacggacctc gaacaccgca cacccgacga ccgtctccgt cggaccgtac tcattgacga
     5401 ccacagatcc cggagcacgc tccagccacg cctgcaccac cgaacccgac agcgcctcac
     5461 caccgaccac ccacgtcgac gcagcaccac tgatctcgcc gtccggaagc atctcggaca
     5521 acagcggcag gtgcgccggc acggccttcg tcagaccgaa accaccacca tcgcgcaaca
     5581 gctcggccag gccctcggca ccgccctcac gactcaccac caccggcgca cccgaaatca
     5641 gcggcacgac cacactcgtc accgtcaggt cgaacgccag cgacgaatgc agcggagcac
     5701 caccacccgc gccataggag ccagccgccc actgggcgta gttggcaagg ccgccgtgcg
     5761 tgacggccac gcccttggga cgacccgtcg agccggacgt gtagatcaca tacgccagac
     5821 cgtccgccga cacggacacc tcgggagcgg tgtcctcaag catcgacaac tggacaccca
     5881 tcagcgtcga gtccagcgcg accatccgcg cacgacccgc cggaagatca cccaggatct
     5941 cctcgtcc
//
