LOCUS       NZ_BMVL01000033         7621 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence33, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000033
VERSION     NZ_BMVL01000033.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 15455)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7834
            Orig. end                         :: 15455
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..7621
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..7621
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7621
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5375](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5375)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            167..757
                     /locus_tag="IE257_RS37335"
                     /old_locus_tag="GCM10010343_76260"
     CDS             167..757
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105931.1"
                     /locus_tag="IE257_RS37335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_76260"
                     /product="hypothetical protein"
                     /protein_id="WP_189974505.1"
                     /transl_table=11
                     /translation="MSALPLNRLSPSAVTDLSALLATCFQEDALTRWMIPGERRRGELL
                     PGLFRVFVELSAAFDGVLTSPDGDAVLLFLPPGAEPDEKALDAAFADVLGEYADAMRTI
                     AGLQEAAHPHGAPHYYVSFGAVRPGRQQSGLMSGLLGRVVARADAEGVGAYVEASSPGG
                     EAAGRRAGFRLLGADIALPDEGPVLRPMWRDPR"
     gene            754..1389
                     /locus_tag="IE257_RS37340"
                     /old_locus_tag="GCM10010343_76270"
     CDS             754..1389
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105932.1"
                     /locus_tag="IE257_RS37340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_76270"
                     /product="DUF6875 domain-containing protein"
                     /protein_id="WP_189974507.1"
                     /transl_table=11
                     /translation="MRGDDLIQSWTAGEIARGEAPAEHLGPLLQVLMWSREFLVSSHPE
                     LGRSGPVCPYTQPSLRKELFHLAVPAAGCEDLASAVDSLRTRHAVLAQRLAPEDRELLT
                     ILLVLPGFDPTDSVELDELQRKAKDEFVAEGLMIGQFHPVCEEPGLYNEAFRPLRSPVP
                     LLAVRKLLVFDLPFLVGDDLHMDHYLQRFAPALPSRIRDQLVSRVVAA"
     gene            complement(1493..2341)
                     /locus_tag="IE257_RS37345"
                     /old_locus_tag="GCM10010343_76280"
     CDS             complement(1493..2341)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (35-126). E-value:
                     1.7e-08. Score: 26.3. Matches aSDomain:
                     nrpspksdomains_IE257_RS37345_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103054.1"
                     /locus_tag="IE257_RS37345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_76280"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_189974520.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.2e-12, bitscore:
                     38.0, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTQHPHTRPYTLNAGGITLSALLALPQDGTPTRATILAIHGRGM
                     RAAYWNSLVPVATSLGHAVLAVDRPGYGDSADRLPEGQTLAGQAETLHAALKEHAADYD
                     IGAGILLLGHSDGGKVALHTAAADGGGTPLLGVDASGVGYHYNPEALHFPSTLGAGATK
                     LNWGPLNLYPRGTFQASRTLLAATPPLESAETLRWTGQYEALAPHVRVPVRLTFAEHEA
                     WWHLEAPTLSAMTSRLTAAPSVTVDHLAASGHNISLGHTAPAYHLRVLTFLEDCLRAAP
                     H"
     aSDomain        complement(1964..2236)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37345_Abhydrolase_1.1"
                     /evalue="1.70E-08"
                     /label="IE257_RS37345_Abhydrolase_1.1"
                     /locus_tag="IE257_RS37345"
                     /protein_end="126"
                     /protein_start="35"
                     /score="26.3"
                     /tool="antismash"
                     /translation="TILAIHGRGMRAAYWNSLVPVATSLGHAVLAVDRPGYGDSADRLP
                     EGQTLAGQAETLHAALKEHAADYDIGAGILLLGHSDGGKVALHTAA"
     gene            complement(2338..3060)
                     /locus_tag="IE257_RS37350"
                     /old_locus_tag="GCM10010343_76290"
     CDS             complement(2338..3060)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958108.1"
                     /locus_tag="IE257_RS37350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_76290"
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="WP_189974522.1"
                     /transl_table=11
                     /translation="MTKDHRTGFPFLEALQASPTPVVMEVKRRDAHGYDMLGGRTPAAI
                     VGAYESAGAPCVSVVTGRWFGGSPALLRDVAALTALPILQKDFITRKDQIRTARELGAS
                     AVLLTAALLPASSMRTLVTECLLTGLTPFVEITNESELATVHHPEECVIAVNNKDIKTR
                     ERGAGDLGRSLDLLPAVRAAGAACPVSASGIDSPHTAAALLDAGFGGLLVGTSLLRTGS
                     PAAWVDAMEGTRKGLAVR"
     gene            complement(3057..3806)
                     /locus_tag="IE257_RS37355"
                     /old_locus_tag="GCM10010343_76300"
     CDS             complement(3057..3806)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958109.1"
                     /locus_tag="IE257_RS37355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_76300"
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="WP_229920905.1"
                     /transl_table=11
                     /translation="MSTTRPLSRTLLKVCGATTPQDIEAAAAAGADCVGLWHGVPGGPS
                     ELDRNGLAALAAAARSTGLLPVLVTFLSDAGELAAIAAHTGVGWVQLHAYQPPALVRAL
                     REALPADVGIVKVLHVQENGGCVERPLIGAYDRAGTDLFLLDTATGDGRIGSTGAALAP
                     DRVTALLPRLTRPFLLAGGLTPANRPAYEDVVHHPGFRGIDVDTAARTTTGGFGVPQIA
                     ALARAWRTGTGTRHAPLPDHPRLELSR"
     gene            complement(3803..4948)
                     /locus_tag="IE257_RS37360"
                     /old_locus_tag="GCM10010343_76310"
     CDS             complement(3803..4948)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105935.1"
                     /locus_tag="IE257_RS37360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_76310"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_189974509.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 3.8e-19, bitscore:
                     59.5, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 4.1e-28, bitscore:
                     87.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.4e-31, bitscore:
                     99.7, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRWNADQADLRDGLERWGDALSADHIELDEQSAFSEDKWKLVQK
                     TGILSLPFDEDHGGLGQSLLTTMYVLEGLGEYNRDAGLSFCITTSICSTGIPLQQFGTT
                     EQKERYLHRICSGEAIGAHAITEAEGGSDAMAMTTRAVRDGDDFVINGSKAFVSNGPIA
                     DVIVVYARTHPDGGPLGTTAFLVDRNTPGLSAGKPIKKMGLRTSPLSELFFDDVRVPKT
                     AVLGRVGGGFLVLDHVMKREILFSFIVNVGEMQHRLERCVEYAKTRKSFGKAIGSYQTI
                     ANKIVDTKIQLETARKWLYDTGEKLEAGENITTDLAISKIVTSESNLATSLTAVQIFGG
                     HGYMAEFGLEQDVRNAVGGTIYSGTNEIQYNRIASMLGLGK"
     gene            complement(5001..5375)
                     /locus_tag="IE257_RS37365"
                     /old_locus_tag="GCM10010343_76320"
     CDS             complement(5001..5375)
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958111.1"
                     /locus_tag="IE257_RS37365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_76320"
                     /product="ectoine synthase"
                     /protein_id="WP_189974511.1"
                     /sec_met_domain="ectoine_synt (E-value: 8e-34, bitscore:
                     106.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRDIETVKTVEWGNGLSRRFLLAEDGLGYSLTDTTVLAGTKSR
                     LEYVNHLEACYCIEGSGEVIELDGTSHEIVPGRMYALDQHDAHFLVASPHEDLRLVCVF
                     SPALRGDEVHSLDAHSSSAY"
     gene            complement(5446..6327)
                     /gene="trpA"
                     /locus_tag="IE257_RS37370"
                     /old_locus_tag="GCM10010343_76330"
     CDS             complement(5446..6327)
                     /EC_number="4.2.1.20"
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840653.1"
                     /locus_tag="IE257_RS37370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_76330"
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="WP_189974513.1"
                     /transl_table=11
                     /translation="MPSPASPARAAGTATAPTATPAPAPFFTRRTPDGPPALALFLNAG
                     DPPLPTLRDLVLMLDEEGVDCLELAVPFPDSVTDGPVIRRSARRALDRGGADLDDVLAF
                     IADVRPHLTHLRIALLADWSHSLKHRDLTTTTREIAAAGADGLLLHALPPRLRATHLES
                     AAAAGLPVVTTCYTKSPPTTQAQAAAQASAYLYLVAHYGRSGTAPATGHAALAQTVTAL
                     RAHTTSPIAVGFGVSTRADVQAVGAAGADAAIIGSAAVAAVERAQEAGTDVTEAFRTFV
                     RSVLPATPHRTA"
     gene            complement(6320..7369)
                     /locus_tag="IE257_RS37375"
                     /old_locus_tag="GCM10010343_76340"
     CDS             complement(6320..7369)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105938.1"
                     /locus_tag="IE257_RS37375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_76340"
                     /product="hypothetical protein"
                     /protein_id="WP_189974514.1"
                     /transl_table=11
                     /translation="MHDALTGLLEQRAPVGRETWRSWWDQLGDGGLDQAQAVALLASLT
                     SRLPDHETLRHLLDSLAERRPATPVTTAWEGTVNVVGTGGGPRTFNISTAAAFVAAAAG
                     VKVVKTGSRAYTSSLGSVDLLERLGIRLTSSRAQTEDTLAAHGIAFAGPFVYPTQLTRL
                     ARTVAPHSMKPFGRFLNALGPFLADLPVTAQVTGVSAAAPLDTLRHLARSTTTRRIWLT
                     SNDTGADELLPFATNTIHGENSLTRIIRPGELTPGDGSFADLRPATDTTAAAAHFLTVL
                     AGDAGRIATRTVCLGAAALIVASGTRRDWLSAVSLAEEAIRSGAAHDLAQRLAAAPAPA
                     PRTLAVTGA"
     CDS             complement(7417..>7619)
                     /NRPS_PKS="Domain: PP-binding (2-44). E-value: 1.3e-07.
                     Score: 23.8. Matches aSDomain:
                     nrpspksdomains_IE257_RS37380_PP-binding.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1147: putative acyl carrier protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064088.1"
                     /locus_tag="IE257_RS37380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_189974516.1"
                     /sec_met_domain="PP-binding (E-value: 1.2e-07, bitscore:
                     22.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding_2 (E-value: 4.3e-05, bitscore:
                     13.8, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TAPAELATDHDLLNDGVIDSLGVLKLIAWVEDRFELAIGDADLDP
                     NNFRSVEAIDTFITESRRTAAA"
     gene            complement(7417..>7621)
                     /locus_tag="IE257_RS37380"
     aSDomain        complement(7486..7611)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37380_PP-binding.1"
                     /evalue="1.30E-07"
                     /label="IE257_RS37380_PP-binding.1"
                     /locus_tag="IE257_RS37380"
                     /protein_end="44"
                     /protein_start="2"
                     /score="23.8"
                     /tool="antismash"
                     /translation="PAELATDHDLLNDGVIDSLGVLKLIAWVEDRFELAIGDADLD"
ORIGIN
        1 gctcgggtga tccgcagtgt cctgatcaaa ccggagtccg agcactacct gccgaccctg
       61 cacgcccgtg tcgcggaact cgcggccgac gcccgggcca cgccctgacc tctccccccc
      121 ttcttgtgtc cttcctccat cccgtcacct gaccggaggc cctgccttgt ctgccctgcc
      181 tttgaaccgg ctgagtccat cggccgtcac cgacctgtcc gccctgctcg ccacctgttt
      241 ccaggaggac gccctgaccc gctggatgat ccccggcgag cggcggcgcg gtgagctgct
      301 gcccgggttg ttccgggtgt tcgtggaact gtccgccgcc ttcgacgggg tgctcaccag
      361 cccggacggc gacgcggtgc tgttgttcct gccgcccggc gccgagccgg acgagaaggc
      421 cctggacgcg gcgttcgccg atgttctggg cgagtacgcg gatgcgatgc gcaccatcgc
      481 gggcctgcag gaggcggccc atccgcacgg cgcgccgcac tactacgtct ccttcggggc
      541 ggtccgaccc ggccggcagc agagcggcct gatgtcgggg ctgctgggcc gggtggtggc
      601 ccgtgcggat gccgagggcg tcggcgccta tgtggaggcc agctccccgg gcggtgaggc
      661 ggccggccgg cgtgccggtt tccggctcct gggcgcggac atcgcgctgc ccgacgaagg
      721 acctgtactg cgacccatgt ggagggaccc ccgatgaggg gagacgatct gatccagtcg
      781 tggaccgccg gtgagatagc ccgcggtgag gctccggcgg agcatctcgg gccgctgctg
      841 caggtgttga tgtggagtag ggagttcctg gtctccagcc atcccgagct ggggcgcagt
      901 ggcccggtgt gcccgtacac gcagccttcg ctgcgcaagg agctgttcca tctggcggtg
      961 ccggcggcgg ggtgtgagga ccttgcctcc gcggtcgact cgctgcgtac gcgtcatgcg
     1021 gtgcttgcgc agcggctggc gccggaggac cgtgagctgc tgacgatcct gctggtgctg
     1081 ccggggttcg atccgacgga ttcggtggag ctggacgagc tgcagcgcaa ggcgaaggac
     1141 gagttcgtgg cggaggggct gatgatcggt cagttccatc cggtgtgcga ggagccgggc
     1201 ctgtacaacg aggcgttccg gccgctgcgt tctccggtgc cgctgctggc ggttcgcaag
     1261 ctgctggtgt tcgatctgcc gttcctggtg ggtgacgacc tgcacatgga tcactatctg
     1321 cagcgtttcg cgccggctct gccgtcgcgg atccgggacc agttggtgag ccgggtggtg
     1381 gccgcctgac agtggtgcgg gcacggctgt ggccccggtg gcgtctgcca ccggggcctc
     1441 ggtctgtcgg gcggtgcgtg gggcggtgcg ggcggtgcgt ggggcgggtg gctcagtgcg
     1501 gggcggcccg caggcagtcc tccaggaagg tcaggacgcg caggtggtag gcgggggcgg
     1561 tgtggcccag gctgatgttg tggccggagg cggccaggtg gtcgacggtg acggagggtg
     1621 cggccgtcag tcgggaggtc atcgcggaca gggtgggggc ctccaggtgc caccaggcct
     1681 cgtgttcggc gaaggtgagg cggacgggga cgcggacgtg gggggccagt gcctcgtact
     1741 ggccggtcca gcgcagggtt tccgcggact cgagcggtgg tgtcgcggcg agcagggtgc
     1801 ggctggcctg gaaggtgccg cgcgggtaga ggttcagggg gccccagttg agtttggtgg
     1861 cgccggcgcc cagggtggag gggaagtgca gggcctcggg gttgtagtgg tagccgacgc
     1921 cggaggcgtc cacgccgagc aggggggtgc cgccgccgtc ggcggctgcg gtgtggaggg
     1981 cgaccttgcc gccgtcggag tggcccagca ggaggattcc ggcgccgatg tcgtagtcgg
     2041 cggcgtgctc cttgagggcg gcgtggaggg tttcggcctg gccggccagg gtctggccct
     2101 cgggcagccg gtcggcggag tccccgtagc cggggcggtc gacggccagg acggcgtggc
     2161 ccaggctggt ggccacgggg acgagggagt tccagtacgc ggctcgcatg ccgcggccgt
     2221 ggatggccag gatcgtggcg cgggtcgggg tgccgtcctg gggcagggcc aggagtgccg
     2281 agagggtgat gccgcccgcg ttgagggtgt agggccgggt gtgggggtgc tgtgtcgtca
     2341 tcggaccgcc agcccctttc gtgtgccttc catggcgtcc acccaggcgg cgggcgagcc
     2401 ggtgcgcagc agtgaggtgc ccacgaggag gccgccgaag ccggcgtcca ggagtgcggc
     2461 ggcggtgtgc gggctgtcga tgccgctggc gctgaccggg caggcggcgc cggcggcgcg
     2521 gacggcgggc agcaggtcca gggagcggcc gaggtcgccg gcgccgcgtt cgcgggtctt
     2581 gatgtccttg ttgttgacgg cgatgacgca ttcctcgggg tggtggaccg ttgcgagttc
     2641 gctctcgttc gtgatctcga cgaagggggt gaggccggtc agcaggcatt cggtgaccag
     2701 ggtccgcatg ctcgacgccg gcagcagggc cgcggtgagc agcacggcgg aggcgccgag
     2761 ctccctggcg gtgcggatct ggtcctttct ggtgatgaag tccttctgga ggatgggcag
     2821 tgcggtcagt gcggccacgt cgcgcagcag ggccggtgag ccgccgaacc agcggcccgt
     2881 caccaccgag acgcagggcg cgcccgcgct ctcgtaggcg ccgacgatgg cggccggggt
     2941 tctgccgccg agcatgtcgt agccgtgggc gtcgcgtcgt ttcacctcca tcaccacggg
     3001 ggtcggggag gcctggaggg cctcgagaaa agggaagccg gtccggtggt ccttcgtcat
     3061 cgcgacagct ccagtcgggg gtggtcgggg aggggggcgt ggcgggtgcc ggtgccggtg
     3121 cgccaggcgc gggccagggc ggcgatctgc gggacgccga agccgccggt ggtggtgcgg
     3181 gcggcggtgt cgacgtcgat gccgcggaat ccggggtggt ggacgacgtc ctcgtaggcg
     3241 gggcggttgg cgggggtcag gccgccggcg aggaggaagg ggcgggtcag gcggggcagc
     3301 agggcggtga cgcggtcggg ggcgagggcg gcgccggtgc tgccgatgcg gccgtcgccg
     3361 gtggcggtgt cgaggaggaa gaggtcggtg ccggcccggt cgtaggctcc gatcaggggg
     3421 cgttccacgc agccgccgtt ctcctggacg tggagcacct tgacgatgcc gacgtccgcg
     3481 gggagtgcct cgcgcagggc ccgtacgagg gcggggggtt ggtaggcgtg gagttggacc
     3541 cagccgacgc cggtgtgcgc cgcgatcgcg gcgagttcac cggcgtcgga caggaacgtc
     3601 accaggaccg gcaggagtcc ggtggagcgg gccgccgccg cgagggcggc gagcccgttc
     3661 cggtccagtt cgctggggcc gccgggtacg ccgtgccaga ggccgacgca gtccgccccg
     3721 gcggccgccg cggcctcgat gtcctgcggg gtggtggccc cgcagacctt gagcagggtc
     3781 cgggacaggg ggcgggtggt gctcacttgc ccagtcccag catcgaggcg atgcggttgt
     3841 actggatttc gttggtgccg gagtagatcg tgccgcccac ggcgttgcgc acgtcctgct
     3901 ccaggccgaa ctcggccatg tagccgtggc cgccgaagat ctgtacggcg gtgaggctgg
     3961 tggcgaggtt ggactcgctg gtgacgattt tggagatggc gaggtcggtg gtgatgttct
     4021 cgccggcctc gagtttttcg cccgtgtcgt agagccactt gcgggcggtc tccagctgga
     4081 tcttcgtgtc gacgatcttg ttggcgatcg tctggtacga accgatcgcc tttccgaacg
     4141 acttacgcgt cttcgcgtac tcgacacacc gttccagccg gtgctgcatc tcccccacgt
     4201 tgacgatgaa ggagaagagg atctcccgtt tcatcacgtg gtccaggacc aggaagccgc
     4261 ctccgacgcg gccgaggacc gcggttttcg gtacccgtac gtcgtcgaag aacagttcgc
     4321 tgagcggtga ggtgcgcagg cccatcttct tgatgggttt gccggcgctc aggcccgggg
     4381 tgttgcggtc gaccaggaag gcggtggtcc cgagcgggcc gccgtccggg tgggtgcggg
     4441 cgtagaccac gatcacgtcg gcgatggggc cgttgctgac gaaggccttg ctgccgttga
     4501 tgacgaagtc gtcgccgtcg cggacggccc tggtggtcat ggccatggcg tcggagccgc
     4561 cctcggcctc ggtgatggcg tgggcgccga ttgcctcgcc cgagcagatc cggtgcaggt
     4621 agcgctcttt ttgttcggtg gtgccgaact gctggagggg gatgccggtg gagcagatgg
     4681 aggtggtgat gcagaagctg aggcccgcgt cgcggttgta ctcgccgagg ccctccagta
     4741 cgtacatggt ggtcaggagt gactgtccga ggcctccgtg gtcctcgtcg aagggcagtg
     4801 agaggatgcc ggtcttctgg acgagcttcc acttgtcctc cgagaaggcg gactgctcgt
     4861 cgagttcgat gtggtctgcg ctgagtgcgt ctccccaccg ctccaggccg tcgcgcagat
     4921 ctgcctggtc ggcgttccaa cggatcacga gggacccctt tgattcggtg atggtgagga
     4981 tgggtggtgc gtcgggtgcc tcagtaggcg gaggagctgt gggcgtcgag gctgtggacc
     5041 tcgtcgccgc gcagggcggg cgagaagacg cagaccaggc gcaggtcctc gtgggggctg
     5101 gcgacgagga agtgggcgtc gtgctggtcg agtgcgtaca tgcggcccgg gacgatctcg
     5161 tgggaggtgc cgtcgagctc gatgacctca ccggagccct cgatgcagta gcaggcctcg
     5221 aggtggttga cgtactccag gcgggacttg gtgcccgcga ggacggtggt gtcggtgagc
     5281 gagtagccca ggccgtcctc ggcgaggagg aagcggcggc tgaggccgtt gccccactcg
     5341 acggtcttca cggtctcgat gtcgcgaacg atcatggcag tctcctggtg tgagggtggt
     5401 gcacgggatg gttcgggtga ggggtggagc tcgctttccc gccggtcagg cggttcggtg
     5461 cggtgttgcg ggcaggacgg agcggacgaa ggtgcggaag gcttcggtca cgtcggtgcc
     5521 ggcttcctgg gcccgttcga cggcggcgac cgcggcggag ccgatgatcg cggcgtcggc
     5581 gccggcggcg ccgacggcct ggacgtccgc tcgtgtgctg accccgaagc cgacggcgat
     5641 cggggacgtc gtgtgcgccc ggagtgcggt gaccgtctgt gccagcgcgg cgtggccggt
     5701 ggcgggtgcg gttccgctgc gtccgtagtg ggcgacgagg tagaggtatg cggaggcctg
     5761 tgcggcggcc tgggcctggg tggtgggggg gcttttcgtg tagcaggtgg tgacgacggg
     5821 caggcctgct gctgcggccg attccaggtg ggtggcgcgc agccgtgggg gcagggcgtg
     5881 gaggaggagt ccgtccgcgc cggcggcggc gatctcgcgg gtggtggtgg tcaggtcccg
     5941 gtgtttgagg gagtggctcc agtcggcgag gagggcgatc cgcaggtggg tgaggtgcgg
     6001 ccgtacgtcg gcgatgaagg cgagtacgtc gtccaggtcg gcgccgccgc ggtccagtgc
     6061 ccggcgggcc gagcggcgga tgacggggcc gtcggtgacc gagtcgggga aggggacggc
     6121 cagttcgagg cagtccacgc cttcttcgtc gagcatgagg accaggtcgc gcagggtggg
     6181 caggggcggg tcgccggcgt tgaggaagag ggcgagtgcg gggggtccgt ccggggtgcg
     6241 gcgggtgaag aagggggcgg gggccggtgt cgcggtcggg gcggtggccg tccccgccgc
     6301 ccgggccggg cttgcggggc taggcaccgg tcaccgccag ggtgcggggg gcgggggccg
     6361 gggcggccgc gaggcgctgt gcgaggtcgt gggcggctcc ggagcggatg gcttcctcgg
     6421 cgagggagac cgccgagagc cagtcgcgtc gggtgccgga ggcgacgatc agggcggccg
     6481 cgccgaggca gacggtgcgg gtggcgatcc ggccggcgtc gccggccagg acggtcagga
     6541 agtgcgcggc ggccgcggtg gtgtcggtgg ccggccgcag gtcggcgaag gagccgtcgc
     6601 ccggggtgag ttcgccgggg cggatgatcc gggtcaggga gttctccccg tggatggtgt
     6661 tggtcgcgaa gggcagcagt tcgtccgcgc cggtgtcgtt ggaggtgagc cagatccggc
     6721 gggtggtggt ggatcgggcg agatggcgca gggtgtcgag gggggcggcc gccgagaccc
     6781 cggtgacctg ggcggtcacc ggcaggtcgg cgaggaaggg gcccagtgcg ttcaggaacc
     6841 ggccgaaggg tttcatgctg tggggggcca cggtccgtgc cagccgggtc agttgggtcg
     6901 ggtagacgaa gggtccggcg aaggcgattc cgtgggcggc gagggtgtcc tcggtctggg
     6961 cgcgggagga ggtgagacgg attccgagcc gctccagcag gtcgacggag ccgagtgagc
     7021 tggtgtaggc gcgggagccg gttttgacga ccttgacgcc ggctgcggcg gcgacgaacg
     7081 ccgcagcggt ggagatgttg aaggtccggg gtccgccgcc ggtgccgacg acgttcacgg
     7141 tgccttccca ggcggtggtc accggcgtcg ccggccggcg ctcggcgagc gagtccagca
     7201 ggtggcgcag ggtctcgtgg tcggggagcc ggctggtcag ggaggccagc agggcgacgg
     7261 cctgggcctg gtccaggccg ccgtcgccga gctggtccca ccaggaccgc caggtctcgc
     7321 ggccgaccgg ggctcgctgt tccagcaggc cggtgagtgc gtcgtgcacg gtcggtctcc
     7381 gtctcgtctg cgtcggggtg gtggtggcgg ggccgctcag gcggccgcgg tgcggcggga
     7441 ttcggtgatg aaggtgtcga tcgcctcgac gctgcggaag ttgttggggt ccaggtcggc
     7501 gtcgccgatg gcgagctcga agcggtcctc gacccaggcg atgagcttga ggacgcccag
     7561 gctgtcgatg accccgtcgt tgagcaggtc gtggtcggtg gcgagttcgg cgggggcggt
     7621 g
//
