LOCUS       NZ_BMVL01000028         8835 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence28, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000028
VERSION     NZ_BMVL01000028.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 26839)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 18004
            Orig. end                         :: 26839
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..8835
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..8835
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8835
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5963](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5963
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(121..1086)
                     /locus_tag="IE257_RS38785"
                     /old_locus_tag="GCM10010343_75690"
     CDS             complement(121..1086)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568306.1"
                     /locus_tag="IE257_RS38785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_75690"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_229920891.1"
                     /transl_table=11
                     /translation="MDIDVLGALAVRENGISITPTAPKPRQVLALLALHADKVVPVSAL
                     IEELWAGEPPRSARTTLQTYVLQLRALIAGALERGTDTDTGAGADIRTRIGTGTGNIQA
                     PGAAPGAAPGAAPGAAAGAGAVGGPAARTAKDILVTLPGGYLLSSGGGTLDVREFDRLA
                     GTGYRAMDAGDFPGAARLLREALALWSGPAFADVQGGIQLDMETRRLEETRLCALDQRI
                     EADLRLGRHRELLAELTVLVTRYRTHENLHGQFMLALHRSGRRGEALDVYQRLRATLVR
                     ELGLEPSVALRRLQRSILMAGPESPAEAHAEPGGERLVRV"
     misc_feature    complement(880..882)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1142..1156
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.18"
                     /tool="antismash"
     gene            complement(1323..2048)
                     /locus_tag="IE257_RS37060"
                     /old_locus_tag="GCM10010343_75700"
     CDS             complement(1323..2048)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643811.1"
                     /locus_tag="IE257_RS37060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_75700"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_189974388.1"
                     /transl_table=11
                     /translation="MVKQERAARTREALVRAAAEVFAEEGFATASIASISRRARVSAGG
                     LHFHFASKNALARAVEDRAERALRQITAGPPAPPLPPATTADGPDTGPDTGGGVGAGGG
                     SLQVLVDSTHALMALLAHDAVVRAGFALCADPSFGSAVDLRRRWRLWVEAVVAAAAGEG
                     LLAEGVSHKDAAHGVVAATVGLEVLGAYDRDWLAPHTLTRFWTLMLPRLASGAALAVLR
                     PQGSPGCGAAPPARNTESD"
     misc_feature    2149..2163
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            complement(2239..2919)
                     /locus_tag="IE257_RS37065"
                     /old_locus_tag="GCM10010343_75710"
     CDS             complement(2239..2919)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991562.1"
                     /locus_tag="IE257_RS37065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_75710"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_189974390.1"
                     /transl_table=11
                     /translation="MARQERAVRTRRAILEAAAAVFDERGYDAATIADILSRAGVTKGA
                     LYFHFSSKQELAQGVLDEQFAEGGVPHRESKLQELVDVAMVLAYRMKREPMLSAGARLS
                     LGPGMRDIFGGGSVPGWIEFTRTLLVRAKEQGELLPHVDPAETAWILSACWTGAQIYSQ
                     TLSDRSDIEERVAAVYQHICPSIATPAMLARLDIAGDRGERVSAEMRRGPGRGATQEAE
                     PALS"
     misc_feature    complement(3020..3040)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.09"
                     /tool="antismash"
     gene            complement(3040..4512)
                     /locus_tag="IE257_RS37070"
                     /old_locus_tag="GCM10010343_75720"
     CDS             complement(3040..4512)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569592.1"
                     /locus_tag="IE257_RS37070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_75720"
                     /product="cytochrome P450"
                     /protein_id="WP_229920892.1"
                     /sec_met_domain="p450 (E-value: 6e-20, bitscore: 61.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRQCPFAIDAFGADVHAEAALVREQAPATRVLLPGGVSAWAVGR
                     SADIRRLLVDPRVSKDAYRHWPAWISGEVDAEWPLAIWVSVQNMITAYGSEHARLRRPA
                     AAAFTARRAAGMRPRIEEVVGELLEDLAARESGEPVDLRTRFAHELPTRIVFELFGIPE
                     RSWAPLHRIINAFFDTNITTEQARRNAADLDVTMADLVAHRREHPGDDMTSALIAARDT
                     TTGTTTTGTDTTGTGTGTGTGTDTTGTGTGTGTGTGTGTDTTGTGTTTTGTGTTGTGTG
                     TGTGTGVGAGAGAGAVGEGARLSEKELVDSLILLFTAGYETSVNLICTALHELLRNPAQ
                     LELVRRGLASWEDVVEEALRIEPPAFYGLLRFAVEDIDMGEGVVIGRGEPILISFGSAG
                     RDPAVHGADAERFDVLRATRGQHLSFGFGTHHCLGATLARMECAVAVRRFFETFPAAAL
                     AGAGELPRVQSFISNGLRELPVVLGPRAPLAD"
     misc_feature    4474..4488
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     misc_feature    4647..4662
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.25"
                     /tool="antismash"
     misc_feature    4977..4992
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.07"
                     /tool="antismash"
     gene            5001..5963
                     /locus_tag="IE257_RS37075"
                     /old_locus_tag="GCM10010343_75730"
     CDS             5001..5963
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106284.1"
                     /locus_tag="IE257_RS37075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_75730"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_229920893.1"
                     /sec_met_domain="AfsA (E-value: 1.1e-23, bitscore: 73.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMLTIGKNAPVLPLPGVEQPWMRCNDARRLTTTVPREYVHRSSL
                     AEVFLTGCERTGEDTFSLRGQWPRAHTFFTSADGTSHDPVQAGETIRQTGLYLCHAVYG
                     VPLGHNVLLWSLDFTSHPEHMRIGRGPSDLDMNALCTGFTWKGNRFSGFLRVDIHRDGH
                     LAATGTARFSCVAPATYRRLRGDRGLHASTRPLPRRTPVPATTTGRLLPFDVVLAPTDR
                     TDRWQLSPDLDHPILFEHANDHHPGMVLVEAARQAASALHTTTTFTPTGIATEFHHYAE
                     LDTPCWIDATHTTPDHITVTGHQDNRTVFRSTITGTTTA"
     gene            complement(8035..>8835)
                     /locus_tag="IE257_RS37080"
                     /old_locus_tag="GCM10010343_75740"
     CDS             complement(8035..>8835)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (11-153). E-value:
                     1.8e-07. Score: 22.5. Matches aSDomain:
                     nrpspksdomains_IE257_RS37080_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013530.5"
                     /locus_tag="IE257_RS37080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_75740"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_229920894.1"
                     /transl_table=11
                     /translation="VEEVWGDLAGGSLRGLCEGVGAVLHLASRIVGTSGELRAVNVEGT
                     RALLVEARRAGVGRFVQLGTAAVYGDGVHRGVVEGEVVERPGSVTSGTRLVGERLVLAA
                     GGTVVRPYLVFGRGDRWVVPGLVRLLGRLPLWVEGGRARVSMISVDALAGALVELAVRR
                     EVMGGVLHAGHPEPVSGRELVETVAGGLGLSLPVGEVDLAGAVELLGGSGGIDPVLVRG
                     VRLLAVDHWYDSGRLWAGLAASPGPRFAEAFGACVPWYRETVLG"
     aSDomain        complement(8374..8799)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS37080_NAD_binding_4.1"
                     /evalue="1.80E-07"
                     /label="IE257_RS37080_NAD_binding_4.1"
                     /locus_tag="IE257_RS37080"
                     /protein_end="153"
                     /protein_start="11"
                     /score="22.5"
                     /tool="antismash"
                     /translation="SLRGLCEGVGAVLHLASRIVGTSGELRAVNVEGTRALLVEARRAG
                     VGRFVQLGTAAVYGDGVHRGVVEGEVVERPGSVTSGTRLVGERLVLAAGGTVVRPYLVF
                     GRGDRWVVPGLVRLLGRLPLWVEGGRARVSMISVDALA"
ORIGIN
        1 tcctgcaccg agtcgtctcc tctcgccccg tcagagtcgc gaactcccgg ccgccggcgc
       61 tctgctgtgt ccggcggacg ggccgggccg tgccctttgc cccctggggg cgggtgggcg
      121 tcagacccgt acgagccttt cgccgcccgg ctccgcgtgg gcctcggccg ggctctcggg
      181 cccggccatc aggatcgagc gctgcaggcg gcgcagggcg accgacggtt ccagtccgag
      241 ttcgcgcacg agggtggcgc gcagtcgctg gtagacgtcc agggcctcgc cgcggcggcc
      301 ggagcggtgc agggcgagca tgaactgtcc gtggaggttc tcgtgggtgc ggtagcgggt
      361 gaccaggacg gtgagttcgg cgaggagttc gcggtggcgt cccagtctca ggtcggcctc
      421 gatccgctgg tccagggcgc acagccgggt ctcctccagc cggcgggtct ccatgtcgag
      481 ctggattccg ccctgtacgt cggcgaaggc gggtccggac cacagggcca gggcctcgcg
      541 cagcaggcgg gcggcgccgg ggaagtcgcc ggcgtccatg gcgcggtagc cggtgccggc
      601 gaggcggtcg aactcgcgga cgtcgagggt tccgccgccg ctgctgagga ggtatccgcc
      661 gggcagggtg accaggatgt ccttggcggt ccgtgccgcc ggccctccga ccgcacccgc
      721 acccgcggcc gcgcccgggg ccgcgcccgg ggccgcgccc ggggccgcgc ccggggcctg
      781 gatgttgccc gtaccggtgc cgattctggt tctgatgtcg gcgccggcgc cggtgtcggt
      841 gtcggtgccc cgttcgaggg cgccggcgat gagggcgcgt aactgcagga cgtaggtctg
      901 gagtgtggtg cgggcactgc gcgggggttc acccgcccac agttcctcta tcagtgccga
      961 tacgggcacc accttatcgg catggagggc caggagggcc aggacctgcc ggggcttggg
     1021 ggcggtcggc gtgatggaga tcccgttctc ccgcacggcc aacgcgccca gtacatcgat
     1081 gtccacgccg tactcccctg tccttggatg agtacagcca gtagaaaaca ggaccggcgg
     1141 tttgtcaata tcaaaccggt cgtgctgttt tgatggtcgg tcatgttcga ctgaccgtca
     1201 aaaacctttc ctggcctggg atttcgagtc ccggaaggtg ctgcggaacg acttcaggag
     1261 gcctcgaccg cccctcgacg agggtcacag gagggtggcc tgccccaccg gaaagagacc
     1321 agtcagtctg attcggtgtt ccgggccggg ggtgcggcgc cgcacccggg cgacccctgc
     1381 ggccgcagga cggccagggc ggcgcccgag gcgagccgcg gcagcatcag ggtccagaac
     1441 cgggtgaggg tgtgcggtgc gagccagtcg cggtcgtagg cgcccaggac ctccaggccc
     1501 accgtcgcgg ccaccacccc gtgcgccgcg tccttgtggc tcactccctc ggcgagcagg
     1561 ccctcccccg ccgcggccgc caccaccgcc tcgacccaca gccgccaccg gcggcgcagg
     1621 tccacggcgc tgccgaagga gggatccgcg cacagggcga aaccggcccg caccaccgcg
     1681 tcgtgcgcga gcagggccat cagcgcatgc gtggagtcga ccagcacctg cagggagccc
     1741 ccgcccgcac cgacaccccc accggtgtcc ggaccggtgt ccggaccgtc cgcggtcgtg
     1801 gccggcggca gtggtggggc tggcgggccg gccgtgatct gccgcagtgc gcgctccgcc
     1861 cggtcctcga cggcgcgggc gagcgcgttc ttgctcgcga agtggaagtg cagtcccccg
     1921 gcgctcaccc gggcccggcg gctgatcgag gcgatcgacg cggtggcgaa gccctcctcg
     1981 gcgaacacct ccgcggccgc ccgcaccagc gcctcccgcg tacgggccgc acgttcctgc
     2041 ttgaccatgg agtctccaca aaccgccaac gcggtttgcc caagggggtg taaagatcgt
     2101 tccggtcaca gtcggcgccg gcgccggagc cccgctcgcg ccccggtgga gccgaccggc
     2161 cccacctgcc ccacctgcac cacccgcacc gtcggatccg tcggggcggg tggtgccggg
     2221 cggggtgccg aaacggcctc aggacagcgc cggttcggcc tcctgcgtcg ctccccggcc
     2281 gggcccgcgc cgcatctccg cgctcacccg ctccccccgg tcgccggcga tgtcgagccg
     2341 ggcgagcatc gcgggcgtcg cgatgctcgg gcagatgtgc tggtagacgg cggccacccg
     2401 ttcctcgatg tcactgcggt cgctcagggt ctgcgagtag atctgcgcgc ccgtccagca
     2461 cgcggagagg atccaggccg tctcggcggg atccacgtgc ggcagcaact caccctgctc
     2521 cttcgcccgg accagcaggg tccgggtgaa ctcgatccag cccggcaccg atccgccgcc
     2581 gaagatgtcg cgcatgcccg gccccaggga gagccgggca cccgcgctga gcatgggctc
     2641 gcgcttcatc cggtacgcca gcaccatcgc gacgtccacg agttcctgca gcttgctctc
     2701 ccggtggggc accccgccct cggcgaactg ctcgtccagg acgccctgcg ccagctcctg
     2761 cttggaggag aagtgaaagt acagggcgcc cttggtcacg cccgcccggg agaggatgtc
     2821 cgcgatggtg gccgcgtcgt agccgcgctc gtcgaagacc gccgccgccg cttccaagat
     2881 cgcgcgacgc gtacgtaccg cacgctcctg ccgtgccacc ggtgcctccg ttgttcatgg
     2941 tccaatacgg ccggatccac cgcgcggatc caccgcccca ttaaaaccaa ccagaaggtc
     3001 cgattctttt caagcaggcc aggcgtcacc ccgacgtgat caatcggcca acggcgcccg
     3061 aggccccagc accacgggca actcccgcag cccgttggag atgaacgact ggacccgcgg
     3121 caactccccc gcccccgcga gagccgccgc cgggaacgtc tcgaagaacc gccgcaccgc
     3181 caccgcgcac tccatccggg ccagcgtcgc ccccaggcag tgatgcgtac cgaacccgaa
     3241 cgacagatgc tgcccgcgcg tcgcacgcaa cacgtcgaaa cgctcggcgt ccgcgccgtg
     3301 cacggcagga tcacgcccgg ccgacccgaa cgagatcagg atcggctcgc cccgcccgat
     3361 caccacaccc tcacccatgt cgatgtcctc gaccgcgaaa cgcagcagcc cgtagaaggc
     3421 cggcggctcg atccgcagcg cctcctcgac cacgtcctcc cacgaggcca gacctcgccg
     3481 caccagctcc aactgggccg gattgcgcag cagttcgtgc agggccgtgc agatgaggtt
     3541 gacggaggtc tcgtagccgg ccgtgaagag caggatcagg ctgtcgacga gttccttctc
     3601 actgagccgc gccccctccc ccaccgcccc ggcacccgcc ccggccccga cgccggtgcc
     3661 ggtgccggtg ccggtgccgg tgccggtggt gccggtgccg gtggtggtgg tgccggtgcc
     3721 ggtggtgtcg gtgccggtgc cggtgccggt gccggtgccg gtgccggtgc cggtggtgtc
     3781 ggtgccggtg ccggtgccgg tgccggtgcc ggtggtgtcg gtgccggtgg tggtggtgcc
     3841 ggtggtggtg tcgcgggcgg cgatgagtgc gctggtcatg tcgtcgccgg ggtgctcgcg
     3901 gcggtgggcg accaggtcgg ccatggtgac gtcgaggtcg gccgcgttgc gccgggcctg
     3961 ttcggtggtg atgttggtgt cgaagaacgc gttgatgatc cggtgcagcg gggcccagga
     4021 gcgctccggg atcccgaaca gctcgaagac gatccgggtc ggcagctcgt gcgcgaaccg
     4081 cgtgcgcagg tccaccggtt caccgctctc ccgcgcggcc aggtcctcga ggagctcgcc
     4141 gacgacctcc tcgatcctgg ggcgcatgcc ggccgcccgg cgggcggtga aggcggcggc
     4201 ggccggtctg cgcagccggg cgtgctcact gccgtaggcg gtgatcatgt tctgcaccga
     4261 gacccagatc gccagcggcc actcggcgtc gacctccccg ctgatccagg ccggccagtg
     4321 acggtaggcg tccttggaga cccgcgggtc caccaggagc ctgcgtatgt cggcggagcg
     4381 gcccacggcc caggcactca ccccgcccgg cagcaggacc cttgtcgcgg gcgcctgttc
     4441 acgcacgagc gcggcctcgg cgtgcacgtc cgcaccaaag gcgtcgatag caaaggggca
     4501 ctgacggttc atgaagaaac tctccagatg gacggcggca gatcggcgac agggtgcagc
     4561 ggcgtgtcca caccccgtat tccaaaaccg cccatccggt ttctgattca ccggcggcgg
     4621 ccccgagtct ggcacgaccc gccccgcatg ccaatggttt tcgggacccg gcccgccgcc
     4681 cgccgcccgc ccgagtcccc ccggacggcg cccggttgaa cggacggcac ccgcaccctg
     4741 tccgaccggc gcatatgacc ggaaggacgg ttcccacttg gggcggtgac gtgcgcgtcc
     4801 cgcagggccg cgccctgtcg gatttccgct ggtccaggtc gtgggagccg tcccgggggg
     4861 cctcccgccg gcccgcccgg ccgacacggg ggccgcatcc tgcccggctg agctccttgc
     4921 aaacaaaacc ggagagtacg tatcttgcgt ctcgcgagcg caacccgtcc caaccgtcga
     4981 acggctctgc gggagaagtc atgaccatgc tgacgatcgg aaagaacgcg cccgtcctgc
     5041 ccctgccggg ggtggaacag ccctggatgc gctgcaacga cgcccgccgg ctgaccacca
     5101 ccgtgcccag ggaatacgtc caccgcagct cactggccga ggtcttcctc accggctgcg
     5161 aacgcaccgg cgaggacacc ttctccctgc gcggacagtg gccccgcgcc cacaccttct
     5221 tcaccagcgc cgacggcacc tcccacgacc cggtccaggc cggcgagacg atccgccaga
     5281 ccggcctcta cctctgccac gccgtctacg gggtccccct cggccacaac gtcctgctgt
     5341 ggagcctgga cttcacctcc caccccgaac acatgcggat cggccgcggc cccagcgacc
     5401 tcgacatgaa cgccctctgc accggcttca cctggaaagg caaccgcttc tccggcttcc
     5461 tgcgcgtgga catccaccgc gacggccacc tcgcagccac cggcaccgcc cgcttcagct
     5521 gcgtcgcccc cgccacctac cgccgactgc gcggcgaccg aggcctccac gcctccaccc
     5581 ggccactgcc ccgccgcaca cccgtccccg ccaccaccac gggccgcctg ctccccttcg
     5641 acgtggtcct cgcccccacc gaccgcaccg accgctggca actcagcccc gacctcgacc
     5701 accccatcct cttcgaacac gccaacgacc accaccccgg catggtcctc gtcgaagccg
     5761 cccgccaggc cgccagcgca ctccacacca ccaccacctt cacccccacc ggcatcgcaa
     5821 ccgaattcca ccactacgcc gaactcgaca ccccctgctg gatcgacgcc acccacacca
     5881 cccccgacca catcaccgtc accggccacc aggacaaccg caccgtcttc cgctccacca
     5941 tcaccggcac caccaccgcc tgaaacccca caccgaacca caccccacaa cccacccccc
     6001 ggccccggac ccgaacaccg gacccgacgc ccgacccgac gcccgaccgg acacccgacc
     6061 ggacacccgg acggacaccg gacacggacg cccgaacgga caactcccct cccccagccg
     6121 ctgttcacgc ccgaccacag cccgacaaca caggcccggc caccgcaccc ggacaccccc
     6181 gcccaggcat cgcaacggac acccggtacg cgaccggaac ccggtacgga ccgatcgacc
     6241 gaaaccccgt acggaaccgg ggccgggcgc ggagccaggg ccgggtacgg gccggtcggc
     6301 ccgagccggg cgcggagccg gggccgggta cgggccggtc ggcccgagcc gggtacggag
     6361 ccggggccgg gtacaggccg gttgcccgga gccgggtacg gagccggggc cgggtacagg
     6421 ccggttgccc ggagccgcgt acggagccgg ggccgggtac aggccggttg cccggagccg
     6481 cgtacgggcc ggttagccgg ggccggatgc ggagccgggg ccgggtacgg gccggttggc
     6541 cggaggccgc gtacggagcc gggcgcggag ccggggccgg gccggttggc cggagccgcg
     6601 tacggagccc gggtacgggt acggaccggt cggccggagc cgggtacagg ccggtcggcc
     6661 ggggccgcgt gcggcggccc gggtacgggc cggttggccg gagccgcgta cggcggcccg
     6721 ggtaccggcc ggttggccgg ggccgcgtgc ggcggcccgg gtacgggccg gttggccgga
     6781 gccgcgtacg gcggcccggg taccggccgg ttggccggag ccgcgtacgg cggcccgggt
     6841 accggccggt tggccggagc cgcgtacggc ggcccgggta ccggccggtt ggccggagcc
     6901 gcgtacggag ccggggccgg gtacgggccg gtcggccgga gccgggtaca ggccgattgg
     6961 ccggggccgc gtgcgggcag ggtgcgggac cgggccggac ggctctcctc ccgtcaccga
     7021 tgtccacacc agagcccgga cccacagcaa aaggggcggg caccgaacag ccaagcaccc
     7081 cgctgctcgg gccgcgcgag caccggagcc cggggcaggc cgagcgtcga agccgggcgc
     7141 cccgggcgcg acccggggcg cgagccagga gtcggccgga acccgacccg ggcgcgaggc
     7201 ctggaacgac ccggaggcag gccagacccg ggcgggagcc gggcgtcgac caggcgcggg
     7261 ccagacccgg gccagggagt cgcccggaag ccgacccggg cgcgaggcct ggaacgaccc
     7321 ggaggcaggc cagacccggg cgggagccgg gcgtcgacca ggcgcgggcc agaccccggc
     7381 cggggagtcg acccgggcag gggcccgaca actcctgcgg tagcaggcac tgttcgcgcc
     7441 ggagcccggc cgggcaccgg gagacggtca ctgaacagga aagtacccgg ccccggcacg
     7501 gcagggcagg gcaggccgat gtggacccgg gcgggagcgg ggagtcgacc aggcgcgggc
     7561 cagaccccgg ccgcggagtc gaacgggcgc gggccggacc cggaccgggg agtcgcccgg
     7621 aagccgaccc gggcgcgagg cctggaacga cccggaggcg ggcccgaccc gggcgggagc
     7681 cgggagtcga ccaggcgcgg gccagacccc ggccggggag tcgacccggg caggggcccg
     7741 acaactcctg cggtagcagg cactgttcgc gccgctgccc ggccggcacc gggagacggt
     7801 cactgaacag gaaagtaccc ggccccggcc cggcagggcc gggcagggcc gggcagggcc
     7861 gacgtggacc cgggcgtccc gcccgggggc cggggccgac aacccacccg gaaacagccg
     7921 atgttcaccc gtgacaggac cgcggcccgg caccggagcg ccgccaccga accgcagaca
     7981 cacccgcccg ggcaaacaca cccgacagca ccccccctcc ccgccggccg gccctcaccc
     8041 caaaaccgtc tcccggtacc acggcacgca cgcaccgaac gcctcggcga accgcggccc
     8101 cggcgaagcc gccaaccccg cccacaaccg cccactgtcg taccagtgat ccaccgccaa
     8161 cagcctcacc ccccgcacca acaccggatc gatccccccc gaaccaccca acaactccac
     8221 cgcccccgcc agatccacct cccccaccgg caacgacaac cccaaccccc cggccaccgt
     8281 ctccaccaac tcccgccccg acaccggctc cggatgcccc gcatgcaaca ccccacccat
     8341 cacctccctg cgcaccgcca actccaccaa cgcccccgcc aacgcatcca ccgaaatcat
     8401 cgaaacccgc gcccgcccac cctccaccca caacggcaac cgacccagca accgcaccaa
     8461 ccccggcacc acccaacgat cacccctccc gaacaccaga tacggccgca ccaccgtccc
     8521 ccccgccgcc aacaccaaac gctcccccac caaacgcgtc ccactcgtca ccgaccccgg
     8581 ccgctccacc acctccccct ccaccacacc ccgatgcacc ccatccccat acaccgccgc
     8641 cgtacccaac tgcacaaacc gccccacccc cgcccgccgc gcctccacca acaacgcccg
     8701 cgtcccctcc acattcaccg cccgcaactc ccccgacgtc cccacgatcc gcgacgccag
     8761 atgcaacacc gcccccaccc cctcacacaa accccgcaac gaaccccccg ccagatcccc
     8821 ccacacctcc tccac
//
