LOCUS       NZ_BMVL01000018         8064 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence18, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000018
VERSION     NZ_BMVL01000018.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 179232)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8064
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..8064
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..8064
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8064
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2092:3064](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2093..3064
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(<1..230)
                     /gene="manB"
                     /locus_tag="IE257_RS32880"
                     /pseudo=""
     CDS             complement(<1..230)
                     /codon_start=1
                     /gene="manB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061191.1"
                     /locus_tag="IE257_RS32880"
                     /note="converts mannose-6-phosphate to mannose-1-phosphate;
                     the resulting product is then converted to GDP-mannose by
                     ManC which is then used in the synthesis of
                     mannose-containing glycoconjugates that are important for
                     mediating entry into host cells; incomplete; too short
                     partial abutting assembly gap; missing C-terminus; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="phosphomannomutase/phosphoglucomutase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPDLSNIVKAYDVRGVVPDEWDESLAELFGAAFVEVTGAGAIVVG
                     HDMRPSSPGLSVAFARGAAARGVDVTLIGLC"
     gene            complement(337..1293)
                     /locus_tag="IE257_RS32885"
                     /old_locus_tag="GCM10010343_67350"
     CDS             complement(337..1293)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="IE257_RS32885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_67350"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_189973497.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.4e-12, bitscore:
                     40.7, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTRHVVVTGATGFVGSAVLRRLAAQDPRAVVRATSRTPPPAAPY
                     GVRWMDADLADEQSLRGVCEGADVLLSLASYIGPDADRCAAVNSAGAAALVAEARRAGV
                     GRIVHLSTCAVYGPGPHRGQDVAELTPAPVSPASAGRLAGEGPVLDAGGLVLRAGLVLG
                     RGDRWAVPALVDAFRRVPARWDGGEGLASFVDVDDLARLIVAFALGDGAAADTTGVLHA
                     HHPEPVRNRDIMDVLAAHRILPALPAEDRPWHRCLDALAATPGWVSERQFTLLARDHWY
                     RADAAWELAGITPGPGPLSRIGPAAGWYRERGGAAPG"
     gene            complement(1290..1991)
                     /locus_tag="IE257_RS32890"
                     /old_locus_tag="GCM10010343_67360"
     CDS             complement(1290..1991)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883955.1"
                     /locus_tag="IE257_RS32890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_67360"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_189973498.1"
                     /transl_table=11
                     /translation="MSERKQQRAPQLRAVQTKAAILRAAAEVFDEFGFSGASISKIMKR
                     ADVTQGGMYFHFSSKEELAYAVMVGQGDGLEFPAGEDGLQHLVDITLYLAEQLQHNPVL
                     RAGVRLAVEQGEFGLRDDVAYQAWVLEFRQQLRFARAKGELQPDVDDHELAWVLVSSFT
                     GAQLFSQASTGRADLPQRIASLWRYLLPAVAADAIRGGLRLTLPPAEQKPRNEPKNASG
                     NETKSGSEKRA"
     misc_feature    2005..2018
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.51"
                     /tool="antismash"
     misc_feature    2006..2019
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.43"
                     /tool="antismash"
     misc_feature    complement(2013..2022)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     gene            2093..3064
                     /locus_tag="IE257_RS32895"
                     /old_locus_tag="GCM10010343_67370"
     CDS             2093..3064
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="IE257_RS32895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_67370"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_189973499.1"
                     /sec_met_domain="AfsA (E-value: 1.5e-23, bitscore: 76.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLSTDHSASPIAALSSALPREYVHKSAHSEVLLTGWRTVAPDEY
                     VVSAQWPRTHSFYTPDGGHHDPLLLAESVRQAIPLLSHVAYEVPFGHRQIWDTFSYSTN
                     PEALAVGSTPADIALHIRCSGISRRGRRLAGLTMHVTATRDGEFLGTAEAGFTNQPEAV
                     YQRLRGRNADLDEVAARTIPLPPPLTPRRVGRDRFHDVVLSPTSSTRRAQLRADVNHPI
                     LFDHPVDHAPGMLLLEAVRQAAYRSAFPRRGVLTDMEIRFFRYAELTSACWIETVPTAG
                     EPATPDRHPVRVVASQNGEVVFTATATINATAPAPLRVPATP"
     misc_feature    2147..2149
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3286..3651)
                     /locus_tag="IE257_RS32900"
                     /old_locus_tag="GCM10010343_67380"
     CDS             complement(3286..3651)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241904.1"
                     /locus_tag="IE257_RS32900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_67380"
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="WP_189973500.1"
                     /transl_table=11
                     /translation="MTENPLPPCPECAGAYTYEMGALLVCPECGHEWPHASGGSGESGG
                     AAEERVIKDAVGNVLADGDTVTVVKGLKVKGSPSGIKAGTKVRNIRLVEGVDGHDIDCR
                     IEGFGPMQLKSSVVRKV"
     gene            complement(3644..4348)
                     /locus_tag="IE257_RS32905"
                     /old_locus_tag="GCM10010343_67390"
     CDS             complement(3644..4348)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951418.1"
                     /locus_tag="IE257_RS32905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_67390"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_189973501.1"
                     /transl_table=11
                     /translation="MSSPSTPARGRPARLDRTRTVDTALELLDEVGLDALTMRRLADAM
                     DVQAGALYRYFATKGDLLTAMAERMLADLAATRTDGDWSEQLASLARGMRTALLARRDG
                     ARVYAGTHATGPHTLGFAEAVIGVLRTAGFGAQEAARTLMAVAHLTIGHTLEEQAALQS
                     VGGEGAADLERLRLAADPERYPQLAAALPALTSEDFAAHFEFGLSLLLQGLRALDRSPT
                     CGDGPEIYSRGD"
     gene            4413..5960
                     /locus_tag="IE257_RS32910"
                     /old_locus_tag="GCM10010343_67400"
     CDS             4413..5960
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019324529.1"
                     /locus_tag="IE257_RS32910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_67400"
                     /product="monooxygenase"
                     /protein_id="WP_189973502.1"
                     /transl_table=11
                     /translation="MDTDVIVVGSGPTGLLLACELALAGVQTLVVERRTRPQQDSRALT
                     LHPRSLEVMDLRGLAPRFLGLGRILPGWHFAALDTPLDFAALDSRRAHTLFLTQARTEE
                     LLAQRAHELGVEIRRGHTLTGLRQSEDGVEAEVHGPDDGPAGARQILRAAYLVGCDGGR
                     STVREAAGIDFPGTDETLTGVLGDFATITAGPEDLAGARGAGVLAVPLEGGLTRFVYID
                     PERMRTPARVPVTLEEFRGALVRICGSDFGVDRPRWLSRFGNATRLAASYRSGRVLLAG
                     DAAHIHFPAAGQGLNTGLQDAMNLGWKLAAEVKGWAPPGLLDTYHTERHPVGRAVTENT
                     EVQTLLAELPLVDRYRRPAAALRGLFEELLKMPEVNRRFAEQVSALGTAYPATAPGADP
                     LVGRRMPDIALSAAGSGATRAYELLHRGGFVLLRLAGARDPHPHPYEAPGRYPHLSAVT
                     AHAREEHPELDGVREILIRPDGHIAWATRSDDAGERRTGRDAALTAWTSPTGAPCPS"
     gene            complement(5955..6776)
                     /locus_tag="IE257_RS32915"
                     /old_locus_tag="GCM10010343_67410"
     CDS             complement(5955..6776)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049992.1"
                     /locus_tag="IE257_RS32915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_67410"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_189973503.1"
                     /transl_table=11
                     /translation="MTSSDLGDFLRARRAHLRPGDVGIASYGHRRVAGLRREEVAVLAG
                     MSSDYYARLEQGRERGPSSQVLDAISAALRMDEAAREHLFRLAGAAPDGDRPQPREAVG
                     AALRQLLDGYTGTPAFVLNPATDFLAANAMADALFSPFARRDNLARMTFLDPAARRFFV
                     HPDRAAEAVVAGLRHATGLDPRYPRLRALVRELGSVSQDFAALWSAHGVRGKTYETKEL
                     DHPDVGRLSLTFQSFDVRGAPGQQLVVYHAEPDSPSARALTLLGGSTGRPS"
     misc_feature    6808..6822
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(7386..7399)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.75"
                     /tool="antismash"
ORIGIN
        1 gaacacagac cgatcaacgt cacatccaca ccccgcgccg ccgcaccccg cgcgaacgcc
       61 accgacagac ccggcgacga cggacgcatg tcatgcccga ccacgatcgc cccagccccc
      121 gtgacctcga cgaacgccgc accgaacagc tccgccaacg actcgtccca ctcgtccggc
      181 acgacaccac gcacgtcata cgccttgacg atgttcgaaa gatcgggcat ttccactcct
      241 tcatggggtg accggctgtg ctccggacgc tacccgccgc cctgcccgac acggcccgcc
      301 cacgccccat cccgttgagg ctttggcaga tagccgtcac cccggtgccg ccccgcccct
      361 ctcccggtac caccccgccg ccgggccgat ccggctcagc gggccgggcc ccggtgtgat
      421 cccggcgagt tcccaggccg cgtccgcgcg gtaccagtgg tcccgggcca gcagcgtgaa
      481 ctgacgttcg ctcacccagc ccggggtggc ggccagcgcg tccaggcacc tgtgccacgg
      541 ccggtcctcc gcgggcagcg ccggcagtat tcggtgcgcg gcgagcacgt ccatgatgtc
      601 ccggttgcgc accggctccg ggtggtgggc gtgcaggaca cccgtcgtat cggccgcggc
      661 gccgtcgccg agggcgaagg cgacgatgag ccgcgccagg tcgtcgacgt cgacgaagga
      721 ggccagcccc tcccctccgt cccaccgcgc cggtacgcgc cggaaggcgt cgacgagggc
      781 cggtacggcc caccggtcgc cccgtccgag caccaggccc gcgcgcagga ccaggccccc
      841 ggcatcgagc acgggcccct cccccgccag ccgtccggcg ctggccggcg agaccggggc
      901 gggtgtcagc tcagccacgt cctgcccccg gtgcggtccc ggtccgtaga cggcgcaggt
      961 cgacaggtgg acgatccgcc ccactccggc ccgtcgggcc tcggccacca gggctgcggc
     1021 accggcggag ttgaccgcgg cgcaccggtc ggcgtccggt ccgatgtagg aggccagcga
     1081 cagcaggacg tccgcgccct cgcacacccc gcgcagggac tgctcgtcgg ccaggtccgc
     1141 gtccatccag cgcacgccgt acggggccgc ggggggcggc gtacgcgatg tcgcgcgcac
     1201 cacggcccgc gggtcctgcg cggccagtcg gcgcagcacg gccgacccca cgaagcccgt
     1261 cgcgccggtg acgacgacat ggcgggtgct caagcccgct tctccgaacc cgactttgtc
     1321 tcgttcccgg atgcgttctt cggctcgttc ctgggcttct gctcggcggg cggcagcgtc
     1381 aggcgcagtc cgccgcggat ggcgtcggcg gccaccgcgg gcagcaggta ccgccacagc
     1441 gaggcgatcc gctgcggcag gtccgcccgt ccggtggacg cctgggagaa gagctgagcc
     1501 ccggtgaacg agctgaccag cacccaggcg agttcgtggt cgtccacgtc cggctggagc
     1561 tcccccttgg cccgggcgaa gcgcagctgc tggcggaact ccaggaccca ggcctggtag
     1621 gcgacgtcgt cgcgcaggcc gaactcgccc tgctccacgg ccagtcgtac gccggcccgg
     1681 agcaccggat tgtgctggag ctgctcggcg aggtagagcg tgatgtccac caggtgctgc
     1741 aggccgtcct cccctgcggg gaactccagg ccgtccccct ggccgaccat gacggcgtag
     1801 gcgagttcct ccttcgagct gaagtggaag tacatgccgc cctgcgtgac gtccgcgcgc
     1861 ttcatgatct tgctgatgct ggccccgctg aaaccgaact cgtcgaagac ctcggccgcg
     1921 gcgcgcagga tggcagcttt ggtctgaact gccctgagtt ggggcgcgcg ttgctgcttc
     1981 cgttctgaca tgagcaccta actaatctcg acaaaaaaaa cgaacatcct ttattttagg
     2041 acctgcgccg aacaagcacc agctcatgtc cgacgtccag gggggctcaa ccatgactct
     2101 gtcaactgat cactccgcct cacccatcgc cgcgctcagc agcgccttac cgcgggagta
     2161 cgtacacaag agcgcacact cggaagtcct actcaccggg tggcgcaccg tcgcgccgga
     2221 cgaatacgtc gtcagcgccc aatggccccg cacccacagc ttctacacgc cggacggcgg
     2281 ccaccacgat cccctgctcc tcgccgagtc ggtccgccag gccatcccgc tcctgagcca
     2341 cgtcgcgtac gaagtcccct tcggtcaccg ccagatctgg gacaccttca gctactcgac
     2401 caaccccgag gctctcgccg tcggttcgac tccggccgac atcgcactcc acatccgctg
     2461 ctccggcatt tcgcggcgcg gccgaagact cgcgggcctc accatgcacg tgaccgccac
     2521 ccgcgacgga gagttcctgg gcaccgccga ggcgggcttc accaatcagc cggaggccgt
     2581 ctaccagcgg ctgcgcggca ggaacgcgga cctcgacgag gtggccgccc gcaccatccc
     2641 gctgccgccg ccgctgacgc cccgccgggt gggccgcgac cgcttccacg acgtggtcct
     2701 ctcccccacc agctccacgc ggcgcgccca gctccgggcc gacgtcaacc acccgatcct
     2761 cttcgaccac cccgtcgacc acgcccccgg catgctgctc ctggaggccg tgcggcaggc
     2821 cgcgtaccgt tccgccttcc cccgacgggg agtcctcacc gatatggaga tccgcttctt
     2881 ccgctacgcg gagctgacct cggcctgctg gatcgagacc gtcccgaccg ccggtgagcc
     2941 ggccacgccg gaccgccatc ccgtacgcgt ggtcgccagc cagaacggcg aggtcgtctt
     3001 caccgccacc gcgacgatca acgccacggc gcccgcaccc ctccgcgtcc ccgcgacgcc
     3061 ctgaccgctc ggtgaggcac tcggctcatg agcgcgccgg ctcatgagcc cccgccgcac
     3121 cggcccgtcc gctccggaag aacggcgaag gcgcctgctc gggtccgtac ccgacaggcg
     3181 ccttcgttcc ttcacggccg tcgccgtcac cgtcccggtc gccaccgacg tcgccggtgc
     3241 cggtgccggt gccggtgccg ctgccgctgc cgctgccgct gccggtcaga ccttcctgac
     3301 cacgctggac ttcagctgca tggggccgaa gccctcgatc ctgcagtcga tgtcatggcc
     3361 gtcgacaccc tccaccaggc ggatgttgcg caccttggtg cccgccttga tgccgctcgg
     3421 gctgcccttg accttcaggc ccttgacgac cgtcaccgtg tcgccgtccg ccagcacgtt
     3481 gcccaccgcg tccttgatca cccgctcctc ggcggcgccg cccgactcac cggacccgcc
     3541 ggaggcgtgc ggccactcgt gcccgcactc ggggcagacc aggagcgccc ccatctcgta
     3601 ggtgtacgcg cccgcgcatt cggggcaggg aggcaagggg ttctcagtca ccccgcgagt
     3661 atatttcagg cccgtctccg caggtcggcg agcggtccag ggccctcagg ccctgcagga
     3721 gcagggacag cccgaattcg aagtgcgccg cgaagtcctc actcgtgagc gcgggcagcg
     3781 cggcagcgag ctgcggatac cgctcgggat cggccgccag gcgcaggcgc tcgaggtccg
     3841 ccgccccctc accccccacc gactggagcg cggcctgctc ctccagcgtg tggccgatgg
     3901 tcaggtgggc caccgccatc agcgtgcgcg ccgcctcctg cgcgccgaat ccggccgtgc
     3961 gcagcacgcc gatgaccgcc tcggcgaagc cgagggtgtg gggcccggtc gcatgggtgc
     4021 ccgcgtagac ccgtgcgccg tcccggcggg cgagcagcgc cgtgcgcatg ccccgggcga
     4081 gggaggccag ctgctcgctc cagtcgccgt cggtcctcgt cgccgcgagg tcggccagca
     4141 tgcgttccgc catcgcggtc agcaggtcgc ccttggtggc gaagtaccgg tacagcgcgc
     4201 cggcctggac gtccatcgcg tcggcgaggc gccgcatcgt cagcgcgtcg aggccgactt
     4261 cgtcgaggag ttccagtgcc gtgtcgaccg tccgggtgcg gtcgaggcgg gccgggcggc
     4321 cacgggcggg ggttgacggg gagctcatgg tcctcactat agtgaacaac gttcacgtga
     4381 acgctgttca ctaattatgg gaggcatcac cgatggatac cgacgtgatc gtggtgggtt
     4441 cggggccgac cgggctgctg ctcgcctgcg agctggcact ggccggcgtg cagaccctgg
     4501 tcgtcgagcg gcgcacccgc ccgcagcagg actcccgggc gctgaccctg catccgcgca
     4561 gcctcgaagt gatggatctg cgcggcctcg ccccgcgctt cctcgggctc ggccggatcc
     4621 tgcccggctg gcacttcgcc gccctcgaca cccccctgga cttcgccgcc ctggacagcc
     4681 gccgggcgca caccctgttc ctgacccagg cgcgtaccga ggagctgctg gcgcagcggg
     4741 cgcacgagct gggggtggag atacgccgcg ggcacacgct caccggcctg cggcagagcg
     4801 aggacggggt cgaggcggag gtgcacgggc cggacgacgg acctgccgga gcccggcaga
     4861 tcctccgcgc cgcgtacctg gtggggtgcg acggcgggcg cagcaccgtc cgggaagctg
     4921 ccggaatcga cttccccggc accgacgaga ccctgaccgg ggtgctcggg gacttcgcca
     4981 ccatcaccgc cggccccgag gacctggccg gcgcccgcgg cgcgggcgtg ctcgccgtcc
     5041 cgctggaggg cgggctcacc cgcttcgtgt acatcgaccc ggagcggatg cggacaccgg
     5101 cgcgggtacc ggtgacgctg gaggagttcc gcggcgcgct ggtccggatc tgcggctccg
     5161 acttcggggt cgaccggccc cgatggctct cgcgcttcgg caatgccacc cggctggcgg
     5221 cctcctaccg ctccggccgc gtcctcctgg ccggcgacgc cgcccacatc cacttcccgg
     5281 ccgcgggcca gggcctcaac accgggctcc aggacgccat gaacctcggc tggaaactgg
     5341 ccgcggaggt gaagggctgg gctcctcccg gcctcctcga cacctaccac accgagcgcc
     5401 accccgtcgg ccgggccgtc accgagaaca ccgaggtgca gacgctgctg gcggagctgc
     5461 cgctcgtgga ccgctaccgc cggccggccg ccgccctgcg cggcctcttc gaggaactgc
     5521 tgaagatgcc cgaggtcaac cgccggttcg ccgagcaggt ctccgcgctc ggcaccgcct
     5581 acccggccac cgcccccggc gccgatccgc tggtcggccg caggatgccc gacatcgccc
     5641 tgagcgcggc cggttcgggc gcgacacggg cgtacgagct cctgcaccgg ggcggcttcg
     5701 tcctgctccg cctcgccggt gcgcgggacc cgcacccgca cccgtacgag gcccccggcc
     5761 ggtacccgca cctcagcgcc gtcaccgccc acgcacgcga ggagcacccg gagctcgacg
     5821 gggtccgcga gatcctgatc cgccccgacg ggcacatcgc ctgggccacc cggagcgacg
     5881 acgccggtga acgccgtacc ggccgcgacg cggcgctcac cgcatggacg tcgccgaccg
     5941 gggcaccctg cccgtcatga cgggcggccg gtggaaccgc cgaggagggt gagggcgcgg
     6001 gcactcgggc tgtccggttc ggcgtggtag accaccagct gctggcccgg cgccccgcgc
     6061 acgtcgaagg actggaaggt gagcgagagc cggccgacgt cggggtggtc gagttccttg
     6121 gtctcgtagg tcttgccccg gacgccgtgt gcggaccaca gcgcggcgaa gtcctggctg
     6181 accgaaccga gttcgcggac cagggcgcgc agccgcgggt agcgcgggtc gaggccggtc
     6241 gcgtggcgca gcccggccac caccgcctcg gccgcgcggt ccgggtggac gaagaaccgg
     6301 cgggccgcgg ggtcgaggaa ggtcatccgg gcgaggttgt cccgccgcgc gaacggggag
     6361 aagagcgcgt cggccatcgc gttggccgcc aggaagtcgg tggcggggtt gaggacgaac
     6421 gccggggtgc ccgtgtaccc gtccagcagt tgcctcaggg cggccccgac ggcctcccgc
     6481 ggctgcggcc ggtcaccgtc gggcgcggca cccgccagcc tgaacaggtg ttcccgcgcg
     6541 gcctcgtcca tgcgcagggc ggcgctgatc gcgtcgagga cctgcgagga gggaccgcgc
     6601 tcgcgcccct gctccagccg ggcgtagtag tcgctgctca ttccggccag aacggcgacc
     6661 tcctcgcggc gcagccccgc cacgcggcgg tgtccgtagg aggcgattcc cacgtccccg
     6721 ggccgcaggt gagcgcgccg ggcacgcagg aagtctccga gatcgctgct ggtcacacct
     6781 tcagattaga aggcggctcc gcgtggcgga actgccgttc ctcattggcg cgttcccgct
     6841 cgttccggca cccctccgac ctgatgatct tgcgtaggct cgggttcgac cgacccgacc
     6901 tgcccggccg acctgcccgg ccgacccgac ctgcccggcc gatcggcacc ggccgatcga
     6961 gacccacgga ccggattccg ccccccaccg gaggagcatc gtgccccagc ccatgaaccg
     7021 acgactcgcc ctcggcgcag ccttgttggc ggtgatggcc gccgtggctc cggcggccac
     7081 cgcccatgcc gccccgacca cccccgacct cgacgcgctg acccaggcgc tgcggaacac
     7141 gaccgctgcc ggagcgccgg gcgccatggc ccgtttcagc ggccccgacg gggtcagcgt
     7201 caagacggtc ggcgtgcgcg acaagatcac ggggtcggcg atggacgccc gggcccgctt
     7261 ccggatcggg agcgtcacca agacgttcac ctccgtcgtc ctgctgcagc tggtggacga
     7321 gggcaaggtc gatccggaca cctccgtcaa ccggtacctc cccgggctgc tccccgacga
     7381 ccggatcacg gtgcgtcacc tgctgagcca ccgcagcggg ctgtcggact acaccaatgc
     7441 catgttcgag cacaccgtgc cgggcttcga ggccgtacgc aaccgggtgt tcggctacca
     7501 ggagctggtg aacctctcgc tcgccgaacc gcggacgaac gagcccggcg ccgcgtacgc
     7561 gtactcgaac accaacttcg tcgtcctcgg catgctcatc gagaaggcca ccggcaacgc
     7621 cgtggccaag gagtacgagc ggcgcatctt caagccgctg aaactgcgca acacctccta
     7681 tgtgcacccc gggacacaga tcaggggtcc gcacgcgcac ggctacctcc accccgacga
     7741 ggcgggcgcc ccgctggtcg actccaccga gcagacggtg tcgtgggccc agtccgcggg
     7801 ggcggtgatc tccgatccgg ccgacctgaa caccttcacg tccgcgctgc tcggcggccg
     7861 gctgctgccc gcgcgcctgc tggacgccat gctcaccgtg accccgaccg acaccacgaa
     7921 cacccggttc tacgggctcg gcctgcgccg ctacgacctg tcgtgcggca cttcggtcta
     7981 cgggcacacg ggcaccgtgc agggcttcta cacctacgcc ttctccaccc gcgacgggcg
     8041 ccgcagtctc gcggcgatgg cgaa
//
