LOCUS       NZ_BMVL01000004        11338 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence04, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000004
VERSION     NZ_BMVL01000004.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 640938)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 71562
            Orig. end                         :: 82900
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11338
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11338
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11338
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6338](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6338)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(588..602)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     gene            1133..1846
                     /gene="hemQ"
                     /locus_tag="IE257_RS11430"
                     /old_locus_tag="GCM10010343_23550"
     CDS             1133..1846
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962204.1"
                     /locus_tag="IE257_RS11430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23550"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_052876931.1"
                     /transl_table=11
                     /translation="MTAPEKIPNAGKKAKDLNEVIRYTLWSVFKLKDVLPEDRSGYADE
                     VQELFDQLGAKDITVRGTYDVSGLRADADVMIWWHAETADELQTAYNLFRRTRLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADEVARDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWMLAFEADELYRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKSVADLVAGLA"
     gene            complement(1977..3605)
                     /locus_tag="IE257_RS11435"
                     /old_locus_tag="GCM10010343_23560"
     CDS             complement(1977..3605)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-464). E-value:
                     2.7e-18. Score: 58.4. Matches aSDomain:
                     nrpspksdomains_IE257_RS11435_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643866.1"
                     /locus_tag="IE257_RS11435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23560"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_229920280.1"
                     /sec_met_domain="PF00561 (E-value: 2.9e-25, bitscore: 84.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAALSLLLCAPAHRPAHAAPSAPPGPLGGTAAEAAGAALVARRAA
                     ARGSDGLGFGRCPEAEDLPGPIRCATLRVPLDYARPDGPQISLTVSRIAATGARDAVRQ
                     GALVHNPGGPGASGMYFPLVADLPGWERIAAAYDLVGYAPRGVGRSAPLSCQDPATRAA
                     GPTRVPAEPSPAYKRQRLAAARAYARGCARRAGAALPYYTTLNNVRDLDVLRAALGERK
                     LSFTGASYGTYLGAVYATLYPGRVRRMVLDSAVDPDPRRIWYRNNLDQSAGFERRWYDF
                     RAWAARHHDTYGLGATPAAVQASYERVRDAVARKPAGGVVGTGELRAAYLQAAYYDDVW
                     PDRAAALAAHLRGDPAPLTTQAAPDPDPETAAGAENATAVYTAVLCNDASWPADWETWD
                     RDHTELARRAPFETWANAFINLPCASWPVRERQRPVAVGVQPERLPPTLVVAAERDGAT
                     PYPGALELQRRLGAGASLVTEAGAGSHGVVGGRNHCVDRHVERYLLTGATPGWRVTCAP
                     HPEPAPVSLDDRAASARRALLPPVV"
     aSDomain        complement(2214..3290)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS11435_Abhydrolase_1.1"
                     /evalue="2.70E-18"
                     /label="IE257_RS11435_Abhydrolase_1.1"
                     /locus_tag="IE257_RS11435"
                     /protein_end="464"
                     /protein_start="105"
                     /score="58.4"
                     /tool="antismash"
                     /translation="ALVHNPGGPGASGMYFPLVADLPGWERIAAAYDLVGYAPRGVGRS
                     APLSCQDPATRAAGPTRVPAEPSPAYKRQRLAAARAYARGCARRAGAALPYYTTLNNVR
                     DLDVLRAALGERKLSFTGASYGTYLGAVYATLYPGRVRRMVLDSAVDPDPRRIWYRNNL
                     DQSAGFERRWYDFRAWAARHHDTYGLGATPAAVQASYERVRDAVARKPAGGVVGTGELR
                     AAYLQAAYYDDVWPDRAAALAAHLRGDPAPLTTQAAPDPDPETAAGAENATAVYTAVLC
                     NDASWPADWETWDRDHTELARRAPFETWANAFINLPCASWPVRERQRPVAVGVQPERLP
                     PTLVVAAERDGATPYPGAL"
     misc_feature    complement(3782..3796)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.56"
                     /tool="antismash"
     misc_feature    3869..3884
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 15.87"
                     /tool="antismash"
     gene            complement(3958..5004)
                     /locus_tag="IE257_RS11440"
                     /old_locus_tag="GCM10010343_23570"
     CDS             complement(3958..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072528.1"
                     /locus_tag="IE257_RS11440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23570"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_189967486.1"
                     /transl_table=11
                     /translation="MNLLAVAIWIAVLTSSLLLITGLRRSRSAPAGPAPGLHDLSEAAF
                     VVGGPGTVVDAALVSMLGDGRLVVGGPGIVQVHPGARATDPAERAVLQAHRGAPSGWLY
                     QVRYAAMCDPAVQEIGDALAARGLITPPGSGLRRWRRWGLIQALVCALLFPVSMVLTFV
                     ALAFDSGYAVPFIVKVLFALVGGIVVGLVCAARAKSRVTGAGARALRAVRSAHLNDRTP
                     HVQTALFGLRGLRDPYLRQQLVPAVRGTRLAAAQSHTRSHGTAGSTHWSGAEVLPILWC
                     AGSDGGGGGGSSCGSGSGCGGGSGCGSSGSGCGSSGGGSSCSSSSSSCSSSSSCSSSSG
                     SSCGSSSS"
     gene            complement(5001..6338)
                     /locus_tag="IE257_RS11445"
                     /old_locus_tag="GCM10010343_23580"
     CDS             complement(5001..6338)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888544.1"
                     /locus_tag="IE257_RS11445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23580"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_189967488.1"
                     /sec_met_domain="DUF692 (E-value: 6.8e-91, bitscore: 299.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPMAHLGVGIGWRPEIADAVERLTGLDWVEVVAENVCPGHLPES
                     LQRLRERGTRVVPHGVSLGLGGADRPDPAKLAALGERAVALGAPLVTEHIAFVRTSSPV
                     LEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALFSWPGEELTEGQFLTEL
                     VERTGVRLLIDVANLHTNRVNRGEDPAAVLDAIPLEALAYVHVAGGIERGGVWHDTHAH
                     PVPPVVLDLLAELRSRVDPPGVLLERDDDFPAEAELAGELAAIRAVVTAGPPAPERPPT
                     PERPPTPEQPAVAVAGSRPAPAEPRPVEEAARTGVALGQAALLSALVAGTPVPEGFDRR
                     RIRVQARALAAKRADVVAKLAPELPSILGGPDPYREAFLSYAKGRPMTAGYRRDALDFA
                     EDLLIRDLPADPAARRRLTAWWRDRAGAKPPRRIVRWARSLAGRAA"
     gene            complement(6508..7260)
                     /locus_tag="IE257_RS11450"
                     /old_locus_tag="GCM10010343_23590"
     CDS             complement(6508..7260)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267202.1"
                     /locus_tag="IE257_RS11450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23590"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_189967490.1"
                     /transl_table=11
                     /translation="MSSQHTNDISDVPAVGEDSPFRQEHVRRDEAPQFVLPLVVRIEKA
                     EPPARTDALETAARAVLVLLTDERAQGEGEWAAAVRDWQDARIRKVVRRARGAEWRKAE
                     TLPGVTVHGAAAQVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPVAAATEPDLPVLWLN
                     PGLDMSAGKTMAQAGHAAQLAWWELTGAERAAWQESGFRLAVRTAPRERWAELSGSGLP
                     VVRDAGFTEIAPGSATVVADHPALRARL"
     gene            7332..8006
                     /locus_tag="IE257_RS11455"
                     /old_locus_tag="GCM10010343_23600"
     CDS             7332..8006
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267203.1"
                     /locus_tag="IE257_RS11455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23600"
                     /product="AIM24 family protein"
                     /protein_id="WP_189967492.1"
                     /transl_table=11
                     /translation="MKSDLFASENLAQAAVAPGMSLQNAKSVKYNVNGEMLARQGSMVA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMSVRGQGEAWFAHRAGNCFVIDFEPGDALT
                     INGRNVLCFDTTLSYEIKMVKGAGMTGGGLFNSLFTGTGKLAVVCDGHPIIIPVTAQAP
                     VYVDTDAVVGWSAQLETGLHRSQSVGSMIRGGSGEAVQLKLSGEGFVIVRPSEATETAA
                     AH"
     gene            8003..8812
                     /locus_tag="IE257_RS11460"
                     /old_locus_tag="GCM10010343_23610"
     CDS             8003..8812
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267204.1"
                     /locus_tag="IE257_RS11460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23610"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_189968283.1"
                     /transl_table=11
                     /translation="MNLTGVGRRYGRRRPWVLRGITLELPRGALVRIEGGNGGGKSTLL
                     RLLAGIDAPTEGRITGRPPRTGYVPERFPAALPLTAASYLTHLGRVHGLPRAEAARRAA
                     DRLERFGAAAYARTPMVELSKGSSQKVAVAQALLAEPGLLVLDEAWTGLDAPARAELDR
                     AVAERTAAGGTVVFVDHDPRRLAGAADAHHRIEGGTLVPGSVTAGPRVRIHAVGPPGGP
                     LPPGPGVAPAPGGGVLLTVDAEHSDALLRELLTARPPWHIRTVEELR"
     gene            8809..9459
                     /locus_tag="IE257_RS11465"
                     /old_locus_tag="GCM10010343_23620"
     CDS             8809..9459
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="IE257_RS11465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23620"
                     /product="ABC transporter"
                     /protein_id="WP_189967493.1"
                     /transl_table=11
                     /translation="MTALLRYQGALLLRSQRWLAPLITYAAFVAAGIRPGDPALDSLGF
                     AAAGLVPTTAWLVRISVTGEPDAARDCTAAAAGPVRVHAAALLTGLAGALLAGAAAAAV
                     ALLIGDRAGAGAGPAALAGALAVAACALTGAAVGALCSRPLVRRRGYAVLAGTLGSLLA
                     WLLAFSPARGAVSALVTAARSGTAPLPLAPLAGALLLAAAAGAAASLTAARRS"
     gene            complement(9456..10442)
                     /locus_tag="IE257_RS11470"
                     /old_locus_tag="GCM10010343_23630"
     CDS             complement(9456..10442)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983590.1"
                     /locus_tag="IE257_RS11470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23630"
                     /product="hypothetical protein"
                     /protein_id="WP_189967495.1"
                     /transl_table=11
                     /translation="MNSLSRRLRLALTVTAVLLLAGGSLGYVLHARHRERPPGQQADAS
                     FRIEEPGLYFRDTAGGRVARQPGSGLPRVTGGPSCDRFHTAGDHALCLRKLPGVPARTE
                     ALVLDRGLREIRRVTVPGIPNRARVSASGRILSWTTFTTGDSYNTAAFSTRTAILDLRT
                     GYLIKSIEQIPLTLGGARHHDSDVNYWGVTFAADDNRFYATVSTRGATHLVEGDLRAWS
                     AKALRENAECPSLSPDGARIAFKKKVSDDLRAPWRLYVLNLATMREQPLAEPHSVDDQA
                     AWLDDSTLAYALPGQDGRASDIWSVPADGTGPPRLLAPGGSSPASVR"
ORIGIN
        1 cccggcgacc tgggaccgct cgccgcctcc ggggtgctct ccgccgaggg cctggcccgc
       61 atcgaggccg agcggacgct gccgcccacc gagatcggcg aggacgtcgc cgtcggcgag
      121 tacgtcgcgg cccgcctcgg ccgcgaggtc gtcgaccggc tggtggaacc gctgctcggc
      181 ggggtctacg cgggcgacgc ctaccggatc tccatgcggg ccgccgtgcc ccagctcttc
      241 gaggccgccc gcacgcacgc cctcctcggc gacggcgtac gcgaactgca gcgccgggcg
      301 gaggcggcgc cccagcaggc cggccccgtc ttcgccggta tcgagggcgg catcggacgg
      361 ctcccgctcg ccgtggccga ggcctgccgg gcggccgggg cgcggctcgt caccggcgcc
      421 cccgtccgcg aggtcgcccg taccgccgaa ggctggcggg tggtggccga tgccgaggtc
      481 atcgaggccg acgccgtgat cctggccacc ccggccgggg ccgccgcgcg cctgctggac
      541 gggctcgcgc cggccgcggc cgccgagctg cgcggggtcg agtacgcctc gatggccctg
      601 atcacgatgg ccttccgccg ctcggacctg ccggcggccg tctccggcgg cggcgccagc
      661 ggattcctcg tgccgcccgt cgacggccgg accatcaagg cctccacctt ctccagcaac
      721 aagtgggcct gggccggagc ggatccggag ctcttcctgc tgcgcacctc ggtgggccgg
      781 tacgccgacg agagcgacct gctgcgcgag gacggcgagc tcgtcgacgt ctccctcgcc
      841 gacctcggcg cggccgtggg cctggtggcc cggccggtcg cctccaccgt cacccgctgg
      901 gacggcggac tgccccagta cccggtcggc cacctcgacc gcgtcgcccg gatccgcggc
      961 gccgtcgcag ccctgccggg cctcgcggtg tgcggcgcgg tctacgacgg cgtgggcatt
     1021 ccggcctgca tcgcgagcgc cggcagggcc gcggaagcag tgatcgccgc gctgggtgcg
     1081 cgtacggcac ccctggcacc gaccactgat cagcgcacgg gacaataggc acatgactgc
     1141 accagagaag attcccaacg cggggaagaa ggcgaaggac ctcaacgagg tcatccgcta
     1201 caccctgtgg tccgtcttca agctgaagga cgttcttccc gaggaccgga gcggctacgc
     1261 cgacgaggtc caggagctct tcgaccagct gggcgccaag gacatcaccg tccgcggcac
     1321 ctacgacgtc tccggcctgc gcgccgacgc cgacgtcatg atctggtggc acgccgagac
     1381 ggctgacgag ctgcagaccg cctacaacct gttccgccgc accaggctgg gccgcgcgct
     1441 ggagccggtg tggtcgaaca tggccctgca ccgcccggcc gagttcaaca agtcgcacat
     1501 tccggccttc ctggccgacg aggtcgcccg cgactacgtc agcgtgtacc ccttcgtgcg
     1561 cagttacgac tggtacctgc tgcccgacga ggaccgccgc cgtatgctcg ccgaccacgg
     1621 caagatggcg cgtggctacc ccgacgtgcg cgccaacacc gtcgcgtcct tctcgctggg
     1681 cgactacgag tggatgctgg ccttcgaggc ggacgagctg taccgcatcg tcgacctcat
     1741 gcgtcacctg cgggcctccg aggcgcgtat gcacgtccgt gaagaggtgc ccttctacac
     1801 cgggcgccgc aagtccgtcg ccgacctggt cgccgggctc gcctgatacc tcccctgggg
     1861 ggaaggaccg gaggacgacc acaccgtcag gtgagttcct ccggtggatc gggaggctgg
     1921 tccgaaagac ggcccctgcc cccgaaacga cgcagccgga gatcccgccc ggaagttcag
     1981 acgactggtg gcagcagcgc cctgcgggcg ctcgctgccc ggtcgtccag cgacaccggt
     2041 gcgggctccg gatgcggcgc gcacgtgacg cgccaccccg gggtggcgcc cgtcagcaga
     2101 taccgctcca cgtggcggtc cacgcagtga ttgcgcccgc cgaccactcc gtgcgagccg
     2161 gcccccgcct ccgtcaccag tgaagccccg gcgccgagcc gccgctggag ttccagcgcg
     2221 cccgggtacg gggtcgcccc gtcccgctcc gccgcgacca ccagggtcgg cggcagccgc
     2281 tccggctgga ccccgaccgc caccggccgc tgccgctcgc gcaccggcca ggaggcgcac
     2341 ggcaggttga tgaaggcgtt ggcccaggtc tcgaaggggg ccctgcgggc cagttcggtg
     2401 tggtcgcggt cccaggtctc ccagtccgcg ggccacgagg cgtcgttgca cagcaccgcc
     2461 gtgtacaccg ccgtcgcgtt ctcggccccg gccgccgtct ccgggtcggg gtcgggggcg
     2521 gcctgggtgg tgagcggcgc cggatcgccg cgcaggtggg ccgcgagggc cgccgcccgg
     2581 tccggccaca cgtcgtcgta gtacgcggcc tggaggtagg cggcccggag ctcgccggtg
     2641 ccgacgaccc cgcccgccgg tttacgggcc accgcgtccc ggacgcgttc gtagctcgcc
     2701 tgcacggccg cgggggtggc gccgaggccg tacgtgtcgt ggtgccgggc cgcccatgcg
     2761 cggaagtcgt accagcggcg ctcgaagccg gcggactggt ccaggttgtt gcggtaccag
     2821 atgcggcgcg ggtcgggatc gacggccgag tcgaggacca tccggcggac ccggcccggg
     2881 tacagggtcg cgtagacggc cccgaggtag gtgccgtacg aggcacccgt gaagctcagc
     2941 ttccgctcgc cgagcgcggc ccgcaggacg tccagatcgc ggacgttgtt gagcgtcgtg
     3001 tagtacggca gggccgcgcc ggcccgccgc gcgcagccgc gggcgtaggc ccgggcggcc
     3061 gcgaggcgtt gcctcttgta cgccggggag ggctcggccg gtacccgggt ggggcccgcg
     3121 gcccgggtgg cggggtcctg gcaggacagc ggggcggagc ggcccacgcc gcgcggggcg
     3181 tagccgacga ggtcgtaggc ggcggcgatc cgctcccagc ccggcaggtc ggcgacgagc
     3241 gggaagtaca tgccggaggc tcccgggccg ccggggttgt ggaccagcgc gccctggcgg
     3301 acggcgtcgc gggcgccggt ggcggcgatc cggctgacgg tgagggagat ctgcgggccg
     3361 tcggggcggg cgtagtcgag cggtacgcgc agggtggcgc accggatcgg gccggggagg
     3421 tcctcggcct cggggcaccg gccgaagccg aggccgtcac tgccccgggc cgcggcgcgg
     3481 cgggcgacga gggcggcgcc ggcggcctcg gccgccgtcc cgccgagggg gccgggcggg
     3541 gcgctcggtg cggcgtgggc cggccggtgg gccggggcgc agaggaggag ggagagcgcc
     3601 gccacggcgg ctgtgcgccc cgccaggccg tctcttccgg atcctgccgg gcccgcggcg
     3661 ccgggcaccg cacctcgctg cgttgtcggg cccgccccag gacgtccagt acaaggacgc
     3721 ccctccgcct tgcaaggacg accttccgcc ttgcgatgca cggcaccgga cgccgcgggc
     3781 tcggccgaca agatccgaaa gagacggccc agtcccgtcc gcatggcggc cccttcctct
     3841 catccgatga accgatgaga ggcctgggtt ccgcacttgt aaaggatcat cgccgggtgt
     3901 cggcgccggt ggtcccgttg ccgcgttttc cgcccccggt tgccgtaccc acggcggtca
     3961 ggagctgctg ctgccgcagc tggagccgga cgagctgctg cagctcgacg aactgctgca
     4021 gctggagctg ctgctcgagc agctggaacc gcctccggag gagccgcagc cgcttcccga
     4081 ggagccgcag cccgaaccgc cgccgcagcc cgaaccgctg ccgcagctcg acccgccccc
     4141 gcccccgccg tcgctcccgg cgcaccagag gatcggcagc acctcggccc cggaccagtg
     4201 cgttgagccg gccgtgccgt gggagcgcgt gtgcgactgg gccgcggcca gccgggtacc
     4261 gcgcacggcg ggcaccaact gctggcgcag gtaggggtcc cgcaggccgc gcagcccgaa
     4321 cagcgcggtc tgcacgtgcg gggtccggtc gttcaggtgg gcggagcgga ccgcgcgcag
     4381 ggcccgcgcc ccggccccgg tgacccggct cttggcgcgg gccgcgcaga ccaggcccac
     4441 gacgatgccg cccaccaggg cgaacagcac cttcacgatg aacggcaccg cgtagccgga
     4501 gtcgaaggcg agcgcgacga aggtcagcac catcgacacc gggaacagca gcgcgcacac
     4561 cagggcctgg atcagccccc agcgacgcca gcgccgcagc ccggacccgg gcggggtgat
     4621 cagcccgcgg gcggccagtg cgtccccgat ctcctgcacg gccgggtcgc acatggcggc
     4681 gtagcgcacc tggtagagcc atcccgaggg cgccccccgg tgggcctgga ggacggcccg
     4741 ctcggcgggg tcggtggccc gcgccccggg gtgcacctgg acgatgcccg gaccgccgac
     4801 caccagccgg ccgtccccga gcatcgagac gagcgcggcg tccacgacgg tgccggggcc
     4861 gcccaccacg aaggcggcct cggacaggtc gtgcaggccg ggggcgggtc cggcgggcgc
     4921 ggaccgggac cggcggagcc cggtgatcag caggagggag gacgtgagga cggcgatcca
     4981 gatcgccacg gcgaggaggt tcatgccgct ctccccgcga gcgaccgggc ccagcgcacg
     5041 atccggcggg gcggtttggc cccggcccgg tcccgccacc aggcggtgag cctgcgccgg
     5101 gcggccggat cggccggcag gtcccggatc agcaggtcct cggcgaagtc cagcgcgtcc
     5161 cggcggtacc cggcggtcat cgggcggccc ttggcgtagg agaggaaggc ctcgcggtac
     5221 gggtccgggc cgcccaggat cgagggcagc tcgggagcca gcttcgccac cacgtcggcc
     5281 cgcttggcgg ccagcgcccg ggcctgcacc cggatccgcc ggcggtcgaa gccctcgggc
     5341 acgggggtcc cggccaccag cgcggacagc agcgcggcct gcccgagcgc caccccggtc
     5401 cgggccgcct cctcgaccgg acgcggctcg gcgggcgcag ggcgggaccc ggcgaccgcg
     5461 acggccggtt gctcgggggt gggcggccgc tcgggggtgg gcggccgctc gggggcgggt
     5521 ggtcccgccg tcaccaccgc gcggatcgcg gccagttctc cggcgagctc cgcctccgcg
     5581 gggaagtcgt cgtcccgctc cagcaggacg ccgggcgggt ccacccggga gcgcagctcg
     5641 gccagcaggt cgagcaccac cggcgggacc gggtgcgcgt gggtgtcgtg ccagacgccg
     5701 ccccgttcga tcccgcccgc cacgtgcacg tacgcgagtg cctccagcgg gatcgcatcc
     5761 agcacggccg ccgggtcctc gccccggttg acccgattgg tgtgcaggtt ggccacgtcg
     5821 atgagcagcc ggaccccggt ccgctcgacc agctccgtga ggaactgccc ttcggtcagc
     5881 tcctcgcccg gccaggagaa cagcgcggcg atgttctcca gggccagtgg cacgggcagg
     5941 gcgtcctgcg cgatccgtac gttctcgcac agcacgtcca gcgcgtcgcg ggtgcgcggc
     6001 accggcagca ggtgcccggc ctccagcacc ggcgaggagg tccgtacgaa ggcgatgtgc
     6061 tcggtgacga gcggcgcccc cagcgccact gcccgctcgc cgagtgccgc cagcttcgcg
     6121 ggatcggggc ggtccgcccc gccgaggccg agcgagaccc cgtgcggcac cacgcgggtg
     6181 ccccgctcgc gcagccgctg cagggactcg ggcaggtggc ccgggcatac gttctcggcc
     6241 accacctcga cccagtccag gccggtcagc cgctcgacgg cgtccgcgat ctccggccgc
     6301 cagccgatgc ccacccccag gtgtgccatg ggtttcatgt cccctcgccc cctctcgtcg
     6361 caccgtgtgc cgatgtcatg gccccggcgg agtcccgcca acccgcagga ggggacgttc
     6421 agaggaacat ttgaggttcc cgccggccga cgcggagccc ggccgtcgga gccgggccgt
     6481 cggagccggg ctgtcggagc ccagctgtca gagccgggcc cgcagcgccg gatggtcggc
     6541 gaccaccgtc gcgctgcccg gcgcgatctc ggtgaacccg gcgtcgcgga ccaccgggag
     6601 cccgctcccg ctcagctccg cccaccgctc ccgcggggcc gtccgcaccg ccagccggaa
     6661 accggactcc tgccaggcgg cccgctccgc ccccgtcagc tcccaccacg ccagctgcgc
     6721 cgcgtggccg gcctgggcca tggtcttgcc ggccgacatg tccaggcccg ggttgagcca
     6781 cagtacgggc aggtccggct ccgtggccgc ggcgaccggt tccgggtcgt ccagatcggt
     6841 gcccgacacc tggagcttgg ccagctcctt gggccagccg tcgaggggca ccggcgggaa
     6901 gacccgtacc tgcgcggcgg ccccgtggac cgtgacgccc ggcagggtct ccgccttgcg
     6961 ccactccgcc ccgcgggccc gccggaccac cttgcggatc cgggcgtcct gccagtcgcg
     7021 gaccgccgcc gcccactcgc cctcgccctg cgcccgctcg tcggtgagca ggaccagcac
     7081 cgcgcgggcc gccgtctcca gggcgtcggt ccgggcgggc ggctcggcct tctcgatccg
     7141 caccaccagc ggcagcacga actgcggggc ctcgtcgcgc cgtacgtgct cctgccggaa
     7201 gggactgtcc tcgccgaccg cggggacgtc ggagatgtcg ttggtgtgct ggctgctcat
     7261 cccgccaagg atgccagcct ccaccttcag gatgacgccc cgggtgggcg gcccgggtga
     7321 ggatcgcagc catgaagagt gaccttttcg catccgagaa cctggcccag gcggccgtcg
     7381 cccccggtat gagcctgcag aacgccaagt cggtgaagta caacgtcaac ggtgaaatgc
     7441 tggcccgcca gggctcgatg gtcgccttcc gcggcaacct ccagttcgag cgcaagggac
     7501 agggcatagg cggcatgctc aagcgcgccg tcacgggcga gggcctcgcg ctcatgtccg
     7561 tacgcggcca gggcgaggcc tggttcgccc accgggccgg caactgcttc gtcatcgact
     7621 tcgagccggg tgacgccctc accatcaacg gccgcaacgt gctctgcttc gacaccaccc
     7681 tgtcctacga gatcaagatg gtgaagggcg ccgggatgac cggaggcggc ctcttcaaca
     7741 gcctcttcac cggcaccggc aagctcgccg tcgtctgcga cggccacccg atcatcatcc
     7801 ccgtcaccgc gcaggcaccg gtctacgtgg acacggacgc ggtggtcggc tggagcgcgc
     7861 agctggagac gggcctgcac cgctcccagt cggtcggctc gatgatccgc ggcggctccg
     7921 gcgaggcggt ccagctgaag ctgagcggcg agggcttcgt catcgtccgc ccgagcgagg
     7981 ccaccgagac ggcggccgcg cactgaacct gaccggtgtg ggccgccgct acggacggcg
     8041 ccggccctgg gtgctgcggg ggatcaccct ggaactcccg cgcggcgccc tcgtacggat
     8101 cgagggcggc aacggcggcg gcaagtccac cctgctgcgg ctcctcgcgg ggatcgacgc
     8161 cccgaccgag gggcgcatca cgggccgccc gccccgcacg ggctacgttc ccgaacgctt
     8221 cccggcggcg ctgccgctca cggccgcctc ctacctgacc cacctggggc gggtgcacgg
     8281 actgccgcgc gccgaggccg cccgccgggc ggccgaccgg ctggagcggt tcggcgccgc
     8341 cgcgtacgcc cgcaccccga tggtcgaact gtccaagggg agcagccaga aggtggccgt
     8401 cgcccaggcc ctgctcgccg aaccggggct gctcgtcctc gacgaggcgt ggaccggcct
     8461 ggacgctccc gcccgggccg aactggaccg cgccgtcgcc gagcgcaccg ccgccggagg
     8521 caccgtcgtg ttcgtcgacc acgacccgcg gcggctcgcg ggcgctgccg acgcccacca
     8581 ccggatcgag ggcgggaccc tcgtccccgg ctccgttacg gccggacccc gcgtacggat
     8641 ccacgcggtc ggtccgcccg gcggcccgct gcccccgggc ccgggcgtcg cgcccgctcc
     8701 cggcggggga gtcctgctca ccgtggacgc cgaacactcc gacgccctcc tgcgcgaact
     8761 gctcaccgcc cggccgccct ggcacatccg caccgtggag gaactccggt gaccgccctg
     8821 ctccgttacc agggcgccct gctcctgcgc tcgcagcgat ggctcgcgcc cctgatcacg
     8881 tacgccgcct tcgtggccgc cgggatccgc ccgggcgacc cggcgctgga ctccctcggg
     8941 ttcgcggccg ccggcctggt cccgaccacc gcctggctgg tccggatctc cgtgaccggc
     9001 gaacccgatg cggcccgcga ctgcacggcc gcagcggccg ggcccgtacg ggtgcacgcg
     9061 gcggccctgc tcaccggcct ggccggggcg ctgctcgccg gagccgcggc tgctgccgtg
     9121 gccctgctga tcggtgaccg ggccggagcc ggcgccggcc cggccgccct cgccggagcc
     9181 ctcgcggtgg cggcctgcgc cctgaccggc gcggccgtgg gcgccctgtg cagccggccg
     9241 ctggtccggc gtcgcggcta cgccgtcctg gccgggaccc tcggttccct gctggcctgg
     9301 ctgctcgcct tctccccggc ccgcggcgcg gtgtccgcgc tggtcaccgc ggcccgctcg
     9361 ggaacggcgc cgctgccgct cgcacccctg gcgggtgcgc tgctgctggc cgccgcggca
     9421 ggcgcggcgg ccagcctgac cgccgcgcgc aggagttagc gcacgctcgc gggggaagag
     9481 ccgcccgggg cgaggagacg gggtgggccg gtgccgtccg ccgggaccga ccagatgtcg
     9541 ctcgcgcggc cgtcctgtcc gggcagggcg taggcgaggg tcgagtcgtc gagccaggcg
     9601 gcctggtcgt cgacgctgtg cggttcggcc agtggctgct cgcgcatcgt cgccaggttc
     9661 aggacgtaca gccgccaggg tgcccggagg tcgtcggaga ccttcttctt gaaggcgatg
     9721 cgggcgccgt ccggggacag cgagggacac tcggcgttct cccgcagcgc cttcgccgac
     9781 caggcgcgca ggtcgccctc gaccaggtgg gtggcgcccc gggtcgagac ggtggcgtag
     9841 aagcggttgt cgtcggcggc gaaggtcacg ccccagtagt tgacgtcgga gtcgtggtga
     9901 cgggccccgc cgagggtcag cgggatctgc tcgatcgact tgatcaggta gccggtgcgc
     9961 aggtccagga tcgccgtccg ggtggagaac gcggcggtgt tgtaggagtc cccggtcgtg
    10021 aaggtggtcc aggacaggat ccggcccgat gccgagacgc gggcccggtt cgggatgccg
    10081 ggaacggtca ctctgcggat ctcccgcagc ccccggtcga gtacgagcgc ctcggtacgg
    10141 gccgggaccc cgggcagctt gcgcaggcac agggcgtggt ccccggcggt gtggaagcgg
    10201 tcgcaggacg ggccgccggt cacgcggggc agacccgagc cgggttggcg cgcgacccgc
    10261 ccgccggccg tgtcccggaa gtagaggccc ggttcctcga tccggaagga ggcgtcggcc
    10321 tgctgcccgg gcggccgttc gcggtgccgc gcgtggagta cgtaccccag cgagccgccc
    10381 gccagcagca ggaccgcggt gacggtgagg gcgaggcgga gccgacggga gagggagttc
    10441 acggggcctc cggtgcggtc ggggaagtgg cgggcaggtc ggcgggcatg atgcgcgagg
    10501 cggccgcgag cgccccggcc agcgcgaggg ccgcggtcca gagggcgggc ccggaccccc
    10561 agagggtcca ggccgccccg aatccggcgg cgcccagcag ccgggccagc gcctggccgg
    10621 tctgcagcac ggcgagcccg cccgcccggc cggccacggg cagcgcgggc gcggcgagcg
    10681 ccatcaggac cccgtcggtg gtggcgtaga agacgcccag cagggcgagc acccccacga
    10741 cggtcggcgg ggagacgggg gcgagcagca ggacgtacgc gccgagcagg gcggcgtgcc
    10801 cgatcaggaa ggccgtccgg cggccggtgc ggtcggcgag gcggcccgcg gggacggcga
    10861 gcagcaggta gacggcggcg gccccgagcg gaagcagtgt gaacagcgcg ggcggcaggt
    10921 cgagtccgcg caggagcagc aggtagagga aggcatcgcc gatggtggtg gcgccgagta
    10981 cagccgaggc gtacagcacg cggcggaagg ccggatcgcg caggaccgag ggacgcggtg
    11041 gggcggcccg gggcgcgggg ggcgccgcgg cggccggacc gggcgcggcc gggacgtgca
    11101 ccacgagcag gagcacgccg agcaggccga tgcagaaact gacggcgaac accgcgtcat
    11161 aggcgtcggc ggtggcccac agcacggcga acgcggcgag cggccccagc agggcacccg
    11221 tggtgtccat ggcgcggtgc acgccgaagg accggccgag ggtctccggg ggcccgctga
    11281 gcgagatcag ggcgtcgcgc ggtgcggtac ggatgccctt gccgagccgg tcggcggc
//
