LOCUS       NZ_BMVL01000004        12221 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence04, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000004
VERSION     NZ_BMVL01000004.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 640938)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 12221
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12221
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="hydrogen-cyanide"
                     /region_number="1"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12221
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12221
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[4152:7221](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4153..7221
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(63..1469)
                     /locus_tag="IE257_RS11160"
                     /old_locus_tag="GCM10010343_22990"
     CDS             complement(63..1469)
                     /EC_number="3.4.11.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1172: methionine aminopeptidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128213.1"
                     /locus_tag="IE257_RS11160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22990"
                     /product="aminopeptidase P family protein"
                     /protein_id="WP_229920277.1"
                     /transl_table=11
                     /translation="MTDAPADASSDPVPGTPARLNTGSHDRPVSFELSRFMAGNWAASP
                     LPDSARVPGHAVTPARRARLSARFPGERLIVPAGELKVRSNDCDHRFRPHSAYAWLTGL
                     TGEDQAGHVLVLEPSGPHAHEAVLYLRPRSPRADGDGEFYRDRRYGEFWVGRRPDLAEA
                     ERLTGIRCAHLDGLGSPAGRNAATDPELASALSELRLVKDAWEVEQLQLAVDHTTAGFE
                     DVVRALPRALAHPRGERWIEGVFGLRARAEGNGTGYETIAASGAHACTLHWIRNDGRLN
                     GAELLLLDAGVETDTLYTADITRTLPLSGRFSPVQRQVYELVLAAQEAGIAALRPGASF
                     GDFHRAGMRVIAEGLAEWGVLKDAEGDLHRRYTLCSSGHMLGLDVHDCAKARAETYLDG
                     VLEEGQVLTVEPGLYLQPDDETLPVELRGIGVRIEDDLVITSDGARLMSGALPRTVTGI
                     EEWMGHLLEG"
     misc_feature    1516..1532
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.29"
                     /tool="antismash"
     misc_feature    1520..1530
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(1562..1575)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            1645..4035
                     /locus_tag="IE257_RS11165"
                     /old_locus_tag="GCM10010343_23000"
     CDS             1645..4035
                     /EC_number="3.4.24.3"
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743073.1"
                     /locus_tag="IE257_RS11165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23000"
                     /product="collagenase"
                     /protein_id="WP_189967399.1"
                     /transl_table=11
                     /translation="MLKHRAVRSSLLASAVAVTLLATAGQATTQAAESAAPAPFTGAQA
                     APAAPAPAPGPTGNPFDEVDRLANPKDNTPAPAPAPGGQNITGQVPGAATAAAPADDLQ
                     KRGKAPKAAPTAKSVAAGVPCTLDGITGLTPEQFADFLGDQAVLADGCLRGLIWTWDAR
                     LTPVMSDAHVQAVSRRISSLAAAHDGRNSSHLEEMFTYLHAVAYHDYSRTEIDVTDAPT
                     VDAMRRAIADFAGAAHTFDVTPSNARTLREALYAGSAPGLRQHQLGLIKKVLATMDPAH
                     PATHLDAGWGGAALAALSVSYLGVYPGNQDTAFHAAVAADASYRDTFKAFSTYTHLKGT
                     GNAWVVRDALSEYARFGQVEGLRDRVVADLGAMLGPVRAAFGDGSEPWAKIVSWLNVYE
                     ACKPYGVCKEDIEKQLFPYTYTYDNGAIKVRTALDRATVDQLYYASKQVKAQYHRVLGT
                     DQPLAGDPNSTLNIVLYASRADYVNYHPILTGYDTNNGGIYIENGATFYTYQRRVPQDS
                     SLTLEELFRHEYTHYLNGRFAVPGFFGEGEWYEGDRTTAMDEGTAEFFDGATRDNGIAV
                     RKSLVKGIISDTAGGGPRMSVEQLLNATYDGDGFRFYSYAGTFFEFLWTEKPALLREMY
                     THLRANDVVAYDAWRHRMGADTYLQRDYDRFLDAQIAKVDQLFVPNTAFTPNDRLRDSA
                     LASVKSSFAAATYNTPDCVENGDPGKRRFTCTGRITANLKNWRSDDQNFKDMSETVDYF
                     ILDRAGAASNNLADMNCSFGPVEIWTNKVAGTSGYSCEGPLRG"
     gene            4153..5328
                     /locus_tag="IE257_RS11170"
                     /old_locus_tag="GCM10010343_23010"
     CDS             4153..5328
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891271.1"
                     /locus_tag="IE257_RS11170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23010"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_189967401.1"
                     /sec_met_domain="DAO (E-value: 3.3e-66, bitscore: 219.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKRHSLDVVIIGAGVVGAACAYYAARAGLSVAVVDRGPVAGGTT
                     GAGEGNLLVSDKEAGPELDLALLSTRLWTELAAVLPREIEYEPKGGLVVAPDETTVKAL
                     RTFAEGQRAAGVDAVEVAADVLHELEPHLAPGMAGGFHYPQDAQVQPAQAAARLLAASA
                     AEVHLGEEVTQILLDRGAVRGVRTPRRELLAPAVVNAAGTWGGHIASLAGVTLPVLPRR
                     GFVLVTEPLPRVVRHKVYAADYIADVASGSAALQSSAVVEGTPSGPVLIGATRERVGFD
                     RTLSTEALRRLASQAAALFPVLADVRVMRTYHGFRPYLPDHLPAIGPDPRRPGLLHACG
                     HEGAGIGLAPATGLLVAAALTGAAPPLPVHPFRPDRFAADPAAADNPEEQH"
     misc_feature    4172..4186
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.01"
                     /tool="antismash"
     gene            5328..5639
                     /locus_tag="IE257_RS11175"
                     /old_locus_tag="GCM10010343_23020"
     CDS             5328..5639
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309276.1"
                     /locus_tag="IE257_RS11175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23020"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_189967403.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.2e-25, bitscore: 84.5,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHSPRSLVGGTPVAAHEITFDGRELPALPGQSIAAVLWGAGILAW
                     RTTREGGAPRGAFCGIGSCFDCLVTVNGRPNQRACLVPARPGDTVTTQEGTGRADLAV"
     gene            5776..7221
                     /locus_tag="IE257_RS11180"
                     /old_locus_tag="GCM10010343_23030"
     CDS             5776..7221
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850112.1"
                     /locus_tag="IE257_RS11180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23030"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_189967405.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.4e-23, bitscore:
                     78.6, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 7.9e-13, bitscore:
                     43.7, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASAHRAAPARADLAIVGAGPAGLAAAVTAAGLGLRVVLLDAGDR
                     PGGQFYRHPAPGLGAARPEALHHGWAEFATREAALRAHESAGRITYLPLHHVWTVVPVG
                     AAGARRGHGAPATEWILHAVAGHAPEERAVAVTARAVLIATGAYERQLPFPGWTLPGVI
                     GAGGAQAMLKSGLVLPGKRVVVAGSGPLLLAVAGSLAAAGATVPAVVEAASYTAYAGRV
                     PALLRNPGKLVEAAGYGGALARHHVRLLTRHAVTEAHGTERVEAVTVARLDRNWRPLPG
                     TGRRIPCDALAVGHGLVPQLELATGLGCAIRRSPDGTVALELDAEQRTSVPGIWSAGET
                     GGIGGAQLAMIEGELAAHSVAGALPAARLTRSRARLRAFADAMAAAHRPGEGWTGWLRD
                     DAVVCRCEEVPAGRIREAADDLGARDARTVKLLTRAGMGWCQGRMCGPAVAALAGEEGA
                     PDRRPLSCPVPLRHLAELPPAGH"
     misc_feature    complement(7257..7273)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
     misc_feature    7263..7273
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            7297..8211
                     /locus_tag="IE257_RS11185"
                     /old_locus_tag="GCM10010343_23040"
     CDS             7297..8211
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267151.1"
                     /locus_tag="IE257_RS11185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23040"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_189967407.1"
                     /transl_table=11
                     /translation="MTHAHTPAPRTRPWHGIMVATALPLREDLTVDHDAYAEHVAWLIA
                     NGCDGVVPNGSLGEYQTLTDEERARVVRTAVEAAGDGARVMPGVAAYGSAEARRWADQA
                     AEAGAGSVLLLPPNAFRADEDAVRAHYAEVARAGLPVVAYNNPIDTKVDLTPALLARLH
                     ADGSIVAVKEFSGDVRRAYEIAELAPGLDLLIGADDVLLELALAGAVGWIAGYPNALPN
                     SCATLYRAAAAGDLDTALPLYKSLHSLLRWDSKTEFVQAIKLSMDLAGRPGGATRPPRF
                     PLTGETEAAVRAATEKALAEGLN"
     gene            8230..9234
                     /locus_tag="IE257_RS11190"
                     /old_locus_tag="GCM10010343_23050"
     CDS             8230..9234
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="IE257_RS11190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23050"
                     /product="proline racemase family protein"
                     /protein_id="WP_189967409.1"
                     /transl_table=11
                     /translation="MRTRHVYHAVDSHTEGMPTRVITGGVGVVPGATMAEKRLHFIEHM
                     DHVRTLLMYEPRGHSAMSGAILQPPTRPDADYGVLYIEVSGLLPMCGHGTIGVATVLVE
                     TGMVPVVEPVTTVRLDTPAGLVSVDVRVEDGAATAVTLTNVPSFGVGLDLKTEVPGFGA
                     ITYDLAYGGNFYAFVELDALGLPFDRARGDDLIAAGLAVMDAINAGPDRPVHPENPSIA
                     GVKHVYLAAPGSDAHRSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGLLELGADFVNE
                     SFIGTEFTGRLVGETTVGGLPAVVPTVTGRAWITGTAQYFLDPTDPFPGGFLL"
     gene            9231..10250
                     /locus_tag="IE257_RS11195"
                     /old_locus_tag="GCM10010343_23060"
     CDS             9231..10250
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965664.1"
                     /locus_tag="IE257_RS11195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23060"
                     /product="proline racemase family protein"
                     /protein_id="WP_229920278.1"
                     /transl_table=11
                     /translation="MITVRTVDYHTAGEPFRIVDCAPSGMPPVPGDTVAERCATVIGPG
                     GSGTAPRRGALDDVRRLLVQEPRGHAGMYGGFVVPADDDGAHFGVLFWHKDGYSTACGH
                     GAMALGVWAVDTGRVAAPDDGDAPVRIDVPSGRVTATVHRGAGRTTGVTFRNVPALVGA
                     RKVPVATSFGLAEVDIAHAGACYAAVRARDLGLDVSRAALPALVRAGQEIRAALATHPA
                     TWHPGGPLLSGVYGVILHEELPDTPFGPHQRNVTVFADGQIDRSPCGSGTSARLALLAE
                     DGRLGPGEDLLHESVTGTVFTGRMLEGGVTEVTGTAHRTGEHAFVVDPHDALGTGFLL"
     gene            10280..11188
                     /locus_tag="IE257_RS11200"
                     /old_locus_tag="GCM10010343_23070"
     CDS             10280..11188
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267154.1"
                     /locus_tag="IE257_RS11200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23070"
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="WP_189968278.1"
                     /transl_table=11
                     /translation="MAALLTPAAAADAVASALLGGLDPEVCPPRSAIGVPGGGELLLMP
                     AASGAYAGVKIAGVAPGNPARGLPRITGSYLLLDGPTLRPLALLDGAALTTLRTPAVSA
                     LALRYLAPAGRPLRLVLFGSGPQAYAHLEAVLAVRELAEVVVVARHPGRAEGLAAYART
                     LGPAARTGTPKEVADADLVICCTTAREPLFDGRLVGPGATVVAVGSHEPTARETDTALV
                     RRAAVYVESRAAALREAGDLLVPEAEGAIGPGHITGTLADLVGGRMPAGGPLSCPQLFK
                     SVGMAWEDLAVAVALVEAAGV"
     misc_feature    complement(11199..11209)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            11260..11973
                     /locus_tag="IE257_RS11205"
                     /old_locus_tag="GCM10010343_23080"
     CDS             11260..11973
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938955.1"
                     /locus_tag="IE257_RS11205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_23080"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_030386117.1"
                     /transl_table=11
                     /translation="MGDLKQHSLIKAQERLRDQVGHALRAALIAGELRPGSVYSAPGLA
                     AELGVSATPVREAMLDLAREGLVEPVRNKGFRITEVSERDLDQFTELRTMIEVPTIGRI
                     TKIATAEQLEALRPIAEEIVSSAREHNLIGYLEADRRFHLSLLALAGNDRLVETVGDLR
                     KRSRLYGLTGLDEAGKLVSSAEEHIELLDLMIGGDAEAAQECMLRHLGHVRSLWAQGRD
                     EPVGRVPGGLGSGLQ"
ORIGIN
        1 cacaccgcac tcgacggcca gccaccgatc agccgtgtca acaacgctgc aggtcaatac
       61 acctagccct ccagcaggtg ccccatccac tcctcgatgc cggtgaccgt gcgcggcagc
      121 gcgcccgaca tcaggcgcgc accgtccgag gtgatgacca ggtcgtcctc gatgcgcacg
      181 ccgatgcccc gcagttccac cgggagcgtc tcgtcgtcgg gctggaggta caggcccggt
      241 tccaccgtca ggacctggcc ctcctccagg acaccgtcca ggtaggtctc cgcccgcgcc
      301 ttcgcacagt cgtgcacgtc cagccccagc atgtggccgc tgctgcacag cgtgtaccgc
      361 cggtgcaggt cgccctccgc gtccttcagg acgccccact cggccaggcc ctccgcgatc
      421 acccgcatgc cggcccggtg gaagtccccg aagctcgccc ccgggcggag cgccgcgatg
      481 cccgcctcct gggcggccag gaccagctcg tacacctggc gctggacggg ggagaagcgg
      541 cccgacagcg gcagggtgcg ggtgatgtcc gccgtgtaga gggtgtccgt ctccacgccc
      601 gcgtccagga gtagcagttc ggccccgttc agccggccgt cgttgcggat ccagtgcagg
      661 gtgcaggcgt gggcgccgga cgcggcgatc gtctcgtacc cggtgccgtt gccctcggcc
      721 cgggcgcgca ggccgaagac cccctcgatc caccgctccc cccgcggatg cgccagcgcg
      781 cgcggcaggg cccgtacgac gtcctcgaag cccgccgtcg tgtggtcgac ggccagctgg
      841 agctgctcga cctcccacgc gtccttcacc aggcgcagct ccgagagcgc ggaggcgagt
      901 tcggggtcgg tggccgcgtt gcggccggcc ggtgagccga gtccgtccag gtgggcgcag
      961 cggatgccgg tgaggcgttc ggcctccgcc aggtccggcc ggcggcccac ccagaactcg
     1021 ccgtaccgcc ggtcccggta gaactccccg tccccgtccg cccgcggcga gcgcggccgc
     1081 aggtacagca cggcctcgtg ggcgtgcggg cccgagggct ccaggacgag gacgtggccc
     1141 gcctggtcct cgccggtgag gccggtcagc caggcgtacg cgctgtgcgg gcggaaccgg
     1201 tggtcgcagt cgttcgagcg gaccttcagc tcgccggcgg ggacgatcag ccgctcgccc
     1261 gggaagcggg ccgagagccg ggcccggcgg gccggggtga cggcgtggcc ggggacgcgg
     1321 gcggaatcgg gcagcgggct cgcagcccag ttacccgcca tgaaacggga caactcgaag
     1381 gatacagggc ggtcgtggct gcccgtgttg agccgggcgg gggtgccggg aacggggtcg
     1441 gaggaggcgt cggcgggggc gtcggtcacg aggactccct caagaagatg cgaactggtg
     1501 ggcgcacagg tcttgtcagt gcaatttcac attactatgt tacagctcac accgcggcac
     1561 gttaatcccc gtcaaacgcc gtgtgacgca gggcagttca cgcaccaccc cccacctcac
     1621 ctctctccac atcaggagtc ctcggtgctc aagcacagag ctgtgcgctc ttcccttctt
     1681 gcctccgccg ttgccgtgac cctcctcgcc accgcggggc aggccaccac gcaggccgcc
     1741 gaatccgcgg ccccggcccc cttcaccggc gcacaggccg ccccggccgc ccccgccccc
     1801 gcccccggcc cgacgggaaa ccccttcgac gaggtcgacc gcctggccaa cccgaaggac
     1861 aacaccccgg cgcccgcccc ggccccgggc gggcagaaca tcaccgggca ggtccccggc
     1921 gcggcgacgg ccgccgcccc cgccgacgac ctgcagaagc gcggcaaggc gcccaaggcc
     1981 gcccccaccg cgaagagcgt cgccgcgggc gtcccctgca ccctcgacgg gatcaccggc
     2041 ctcaccccgg agcagttcgc cgacttcctc ggagaccagg ccgtcctcgc cgacggctgt
     2101 ctgcgcgggc tgatctggac ctgggacgcc cgcctgaccc cggtcatgtc ggacgcgcac
     2161 gtccaggccg tctcccgccg gatatccagc ctggccgccg cccacgacgg ccgtaactcc
     2221 tcgcacctgg aggagatgtt cacgtacctg cacgcggtcg cctaccacga ctactcgcgc
     2281 accgagatcg acgtcaccga cgcccccacc gtcgacgcca tgcgccgggc catcgccgac
     2341 ttcgcgggcg ccgcgcacac cttcgacgtg acgccgagca acgcccgcac cctgcgcgag
     2401 gccctctacg ccggcagcgc gcccggcctg cgccagcacc agctgggcct catcaagaag
     2461 gtcctggcca ccatggaccc ggcccacccg gccacccacc tcgacgccgg ctggggcggc
     2521 gccgcgctgg ccgccctgtc cgtctcctac ctgggtgtct acccgggcaa ccaggacacc
     2581 gccttccacg ccgccgtggc cgccgacgcc tcctaccgcg acaccttcaa ggccttctcc
     2641 acctacacgc acctcaaggg caccggcaac gcctgggtgg tgcgcgacgc gctgagcgag
     2701 tacgcccgct tcggccaggt cgagggcctg cgcgaccggg tcgtcgccga cctcggcgcg
     2761 atgctcgggc ccgtccgcgc cgccttcggc gacggcagcg agccgtgggc caagatcgtc
     2821 tcctggctca acgtctacga ggcctgcaag ccgtacgggg tgtgcaagga ggacatcgag
     2881 aagcagctgt tcccctacac ctacacctac gacaacggcg ccatcaaggt ccgcaccgcc
     2941 ctggaccgcg ccacggtcga ccagctgtac tacgcgagca agcaggtgaa ggcccagtac
     3001 caccgcgtgc tcggcaccga ccagccgctc gcgggcgacc ccaactccac gctgaacatc
     3061 gtgctgtacg cctcccgcgc cgactacgtc aactaccacc cgatcctgac cggctacgac
     3121 accaacaacg gcggcatcta catcgagaac ggcgccacct tctacaccta ccagcgccgc
     3181 gtcccgcagg actcctccct caccctcgag gagctcttcc gccacgagta cacgcactac
     3241 ctcaacggcc gcttcgcggt ccccggcttc ttcggcgagg gcgaatggta cgagggcgac
     3301 cggacgaccg ccatggacga gggcacggcc gagttcttcg acggcgccac ccgcgacaac
     3361 ggcatcgccg tccgcaagtc cctggtcaag ggcatcatca gcgacacggc cggcggcggc
     3421 ccgcgcatga gcgtcgagca gctgctgaac gccacctacg acggcgacgg cttccgcttc
     3481 tacagctacg ccggcacctt cttcgagttc ctgtggaccg agaagcccgc gctgctgcgc
     3541 gagatgtaca cgcacctgcg ggccaacgac gtggtggcct acgacgcctg gcgccaccgg
     3601 atgggcgccg acacctacct ccagcgcgac tacgaccgct tcctggacgc gcagatcgcc
     3661 aaggtcgacc agctcttcgt gccgaacacc gccttcaccc cgaacgaccg gctgcgcgac
     3721 tcggcgctcg cctcggtgaa gtcctccttc gcggcggcca cctacaacac cccggactgc
     3781 gtggagaacg gtgaccccgg caagcgccgc ttcacctgca ccggccggat caccgcgaac
     3841 ctgaagaact ggcggagcga cgaccagaac ttcaaggaca tgtcggagac ggtcgactac
     3901 ttcatcctcg accgggcggg tgcggcctcc aacaacctgg ccgacatgaa ctgctccttc
     3961 ggcccggtgg agatctggac gaacaaggtg gcgggcacct ccggttacag ctgtgagggt
     4021 ccgctccgcg gctgaccggc accggtccgc cgccgacccc ggcccggcgc cggaccggtc
     4081 ccgcatccga ggggggtgcg ggaccggtcc gggcgtctgg gcaggcccta gagaatgtga
     4141 catattactg ccgtgctcaa gagacactcc ctggacgtcg tgatcatcgg cgccggcgtc
     4201 gtcggggcgg cctgcgcgta ctacgcggcc cgcgccggac tctccgtggc cgtggtcgac
     4261 cggggccccg tcgcgggcgg caccaccggc gccggcgaag gcaacctgct cgtctccgac
     4321 aaggaggccg gccccgaact cgacctggca ctgctgtcca cccgcctgtg gaccgaactc
     4381 gccgcggtcc tcccgcggga gatcgagtac gagcccaagg gcgggctcgt cgtggccccc
     4441 gacgagacca ccgtcaaggc cctgcggacc ttcgccgagg gccagcgcgc ggccggggtc
     4501 gacgcggtcg aggtcgcggc ggacgtcctg cacgagctgg agccgcacct ggcaccgggc
     4561 atggcgggcg ggttccacta cccgcaggac gcccaggtcc agcccgcgca ggcggcggcc
     4621 cggctgctgg ccgcgtcggc cgccgaggtg cacctcggcg aggaggtgac gcagatcctg
     4681 ctggaccggg gcgccgtacg cggggtgcgc acgccccgcc gtgaactcct ggccccggcc
     4741 gtggtgaacg cggcgggcac ctggggcggg cacatcgcct cgctggccgg ggtcaccctg
     4801 cccgtcctcc cgcgccgcgg cttcgtcctg gtgaccgaac ccctgccgag ggtggtccgg
     4861 cacaaggtct acgccgccga ctacatcgcg gacgtcgcca gcggctccgc cgccctgcag
     4921 tcctcggcgg tcgtcgaggg caccccgtcg ggcccggtcc tgatcggcgc caccagggag
     4981 cgcgtcggct tcgaccgcac cctctccacg gaggccctgc gccggctggc ctcccaggcg
     5041 gcggcactct tccccgtcct ggccgacgtc cgggtcatgc gcacctacca cggtttccgc
     5101 ccctacctgc ccgaccacct cccggcgatc ggccccgacc cgcggcgccc cgggctgctg
     5161 cacgcctgcg gccacgaggg cgccggcatc ggactggccc ccgccaccgg gctgctggtc
     5221 gccgccgctc tgacgggggc cgcaccaccg ctccccgtgc acccgttccg cccggaccgc
     5281 ttcgcggcgg acccggccgc cgccgacaac cccgaggagc agcactgatg cacagccccc
     5341 gctcgctggt cggcggtacg ccggtggccg cgcacgagat caccttcgac gggcgggaac
     5401 tgcccgccct gcccggccag agcatcgccg ccgtgctctg gggcgcgggc atcctcgcct
     5461 ggcgcaccac ccgcgagggc ggagccccgc gcggggcgtt ctgcgggatc ggcagctgct
     5521 tcgactgcct ggtcaccgtc aacggccgcc cgaaccagcg ggcctgcctg gtccccgccc
     5581 gaccggggga caccgtcacc acccaggagg gaaccggccg tgccgacctc gccgtctgaa
     5641 cccgccggtc gcgcgggccg cgcggatccc gcgggcccgt cgggtcccgc caccgcgccg
     5701 acacctgcgc ccaccgccgc gcccgctgcc gtgccccggc ctgccgccca tcccgggccc
     5761 gctgccgcgc ccgtcgtggc gtccgctcac cgggccgcgc cggcccgtgc cgatctcgcc
     5821 atcgtcggag ccggcccggc cggactggcc gccgccgtca ccgccgccgg gctcgggctg
     5881 cgggtcgtcc tcctcgacgc gggcgaccgc ccgggcgggc agttctaccg ccaccccgcc
     5941 ccgggcctgg gcgcggcccg ccccgaggcg ctccaccacg gctgggccga gttcgcgacc
     6001 cgcgaagccg ccctgcgcgc ccacgagtcg gccggccgga tcacgtacct cccgctccac
     6061 cacgtctgga cggtcgtccc ggtcggggcg gccggggcac gccgcgggca cggcgcgccc
     6121 gccaccgagt ggatcctgca cgccgtcgcc ggtcacgccc ccgaggagcg ggccgtggcc
     6181 gtgacggccc gggcggtgct catcgccacc ggcgcctacg agcggcaact gcccttcccc
     6241 ggctggaccc tgcccggggt gatcggcgcc ggcggggcgc aggccatgct caagagcggg
     6301 ctggtgctgc cgggcaagcg ggtggtcgtc gccgggagcg ggccgctgct gctcgccgtg
     6361 gccggctcgc tggccgccgc cggagccacc gtgcccgccg tggtggaggc cgcctcctac
     6421 accgcgtacg cgggccgggt ccccgccctg ctgcgcaacc ccggcaagct cgtcgaggcc
     6481 gccggctacg gcggggcgct ggcccgccac cacgtccggc tcctcacccg gcacgccgtc
     6541 accgaggcgc acggcaccga acgggtcgag gccgtcaccg tggcccggct cgaccggaac
     6601 tggcgtccgc tgccgggcac cggccgccgg atcccctgcg acgccctggc cgtcggccac
     6661 gggctggtgc cccagctgga actcgccacc ggcctcggct gcgccatccg gcgaagcccc
     6721 gacgggaccg tcgccctgga actcgacgcc gagcagcgga cctccgtccc cgggatctgg
     6781 tcggcgggtg agaccggtgg tatcggggga gcccaactgg ccatgatcga gggcgaactg
     6841 gccgcgcact ccgtcgcggg cgcgctgccc gcggcccggc tcacccgcag ccgcgcgcgg
     6901 ctgcgcgcct tcgccgacgc gatggccgcc gcgcaccgcc ccggtgaggg ctggaccggc
     6961 tggctccggg acgacgcggt cgtctgccgc tgcgaggagg tgccggccgg gcggatccgg
     7021 gaagccgcag acgacctcgg cgcgcgggac gcccgtaccg tcaaactcct cacccgggcc
     7081 ggcatgggct ggtgccaggg ccggatgtgc ggtcccgcgg tcgcggccct ggccggggag
     7141 gaaggcgcgc ccgaccggcg tccgctgtcc tgcccggtgc cgctgcgcca tctcgccgaa
     7201 cttccgcccg ccgggcactg atcggggtgc gcgggccctt gtggccgcgc cgcacccact
     7261 agtaaaatgt cacacaccac tctagggagc tctcacatga cccacgcgca cacccccgcg
     7321 ccccgcaccc gtccctggca cggaatcatg gtcgccaccg cgctcccgct gcgcgaggac
     7381 ctcaccgtcg accacgacgc gtacgccgaa cacgtggcct ggctgatcgc caacggctgc
     7441 gacggcgtgg ttcccaacgg ttccctcggc gagtaccaga ccctcaccga cgaggagcgg
     7501 gcgcgcgtcg tgcgtaccgc cgtcgaggcg gcgggcgacg gcgcccgcgt catgcccggc
     7561 gtcgccgcct acggcagcgc cgaggcccga cgctgggccg accaggccgc cgaggcgggc
     7621 gccggctccg tcctcctgct gccccccaac gccttccggg ccgacgagga cgccgtacgc
     7681 gcccactacg ccgaggtcgc ccgcgccggg ctgcccgtcg tcgcgtacaa caaccccatc
     7741 gacaccaagg tggacctgac cccggccctg ctcgcccggt tgcacgccga cggttccatc
     7801 gtcgcggtca aggagttcag cggtgacgtg cgccgcgcgt acgagatcgc cgagctggcc
     7861 cccggcctcg acctgctcat cggcgccgac gacgtcctcc tcgaactggc cctcgcgggc
     7921 gccgtcgggt ggatcgccgg ctaccccaac gcgctcccga actcctgcgc caccctctac
     7981 cgtgccgccg ccgccgggga cctcgacacc gccctgccgc tctacaagtc cctgcactcc
     8041 ctgctgcgct gggactccaa gaccgagttc gtccaggcca tcaagctctc catggacctg
     8101 gccgggcgcc cgggcggcgc cacccgcccg ccccgcttcc cgctcacggg cgagaccgag
     8161 gccgccgtcc gcgccgccac cgagaaggcc ctcgccgagg gcctcaacta gtcaccgggg
     8221 gaaacaggaa tgcgtacgcg ccacgtctac cacgcggtgg actcgcacac cgagggcatg
     8281 ccgacccgcg tcatcaccgg gggagtcggg gtggtccccg gcgccaccat ggccgagaag
     8341 cggctccact tcatcgagca catggaccac gtccggacgc tgctcatgta cgagccgcgc
     8401 gggcactccg cgatgagcgg cgcgatcctc cagcccccga cccgcccgga cgccgactac
     8461 ggcgtcctct acatcgaggt gtcgggcctg ctccccatgt gcgggcacgg caccatcggg
     8521 gtcgccaccg tcctcgtcga gaccggcatg gtgccggtcg tcgagccggt caccaccgtc
     8581 cggctcgaca ccccggccgg gctggtgagc gtcgacgtcc gggtcgagga cggtgcggcc
     8641 accgccgtca ccctcaccaa cgtcccctcc ttcggcgtcg gcctcgacct caagaccgag
     8701 gtccccggct tcggcgcgat cacctacgac ctggcctacg gcggcaactt ctacgccttc
     8761 gtcgaactcg acgccctggg cctgcccttc gaccgagccc ggggggacga cctgatcgcc
     8821 gccgggctcg cggtgatgga cgcgatcaac gccggcccgg accggcccgt ccaccccgag
     8881 aacccctcca tcgcaggcgt caagcacgtc tacctcgccg cccccggctc cgacgcccac
     8941 cgctcccggc acgccatggc catccacccc ggctggttcg accgctcgcc ctgcggcacg
     9001 gggaccagcg cgcggatggc ccagctgcac gcccgcggcc tgctggagct cggcgcggac
     9061 ttcgtcaacg agtccttcat cggcaccgag ttcaccggcc ggctggtcgg ggagaccacc
     9121 gtcggcggcc tcccggccgt cgtccccacg gtcaccggac gggcctggat caccggcact
     9181 gcccagtact tcctcgaccc gaccgacccc ttccccggag ggttcctgct gtgatcaccg
     9241 tccgcacggt ggactaccac accgccggcg agcccttccg gatcgtcgac tgcgccccga
     9301 gcggcatgcc gccggtcccc ggggacaccg tcgccgagcg ctgcgccacc gtcatcggcc
     9361 cgggaggctc cggcaccgcg ccgcgccggg gcgcactgga cgacgtacgg cgcctgctgg
     9421 tgcaggaacc gcgcggacac gccgggatgt acggggggtt cgtggtcccg gccgacgacg
     9481 acggggccca cttcggcgtg ctgttctggc acaaggacgg ctactccacg gcctgcggcc
     9541 acggggccat ggccctcggg gtctgggccg tggacaccgg ccgggtcgcc gccccggacg
     9601 acggggacgc ccccgtgcgc atcgacgtac cgtcggggcg ggtcaccgcc accgtccacc
     9661 gcggcgcggg ccgcaccacc ggggtcacct tccgcaacgt cccggccctg gtgggtgccc
     9721 gcaaggtgcc cgtcgccacc tcgttcgggc tcgccgaggt ggacatcgcg cacgccgggg
     9781 cctgttacgc cgccgtccgc gcgcgggacc tgggcctcga cgtctcccgg gcggcgctgc
     9841 ccgccctggt gcgggccggg caggagatcc gggcggccct ggccacccac ccggccacct
     9901 ggcacccggg cggtccgctg ctctccgggg tctacggggt gatcctccac gaggagctgc
     9961 ccgacacccc cttcgggccg caccagcgca acgtcaccgt cttcgccgac gggcagatag
    10021 accgctcgcc ctgcggttcg ggcacctcgg cgcggctcgc gctgctcgcc gaggacgggc
    10081 ggctcggccc gggcgaggac ctgctccacg aatcggtcac cggcaccgtg ttcaccggcc
    10141 ggatgctgga gggcggggtc accgaggtca ccggcaccgc ccaccgcacc ggcgaacacg
    10201 ccttcgtggt ggacccgcac gacgcgctcg gtaccgggtt cctgctgtga cgggcatccc
    10261 tcagctcgac gccgggcgga tggccgcgct gctcacccct gccgcggccg ccgatgcagt
    10321 ggcctccgcc ctgctcggcg gcctcgaccc cgaggtctgc ccgccgcgct ccgcgatcgg
    10381 ggtgcccggc gggggtgaac tgctgctgat gccggccgcg tccggcgcgt acgccggggt
    10441 gaagatcgcc ggggtcgcgc ccgggaaccc ggcccgcggg ctgccccgga tcaccggctc
    10501 ctacctgctg ctggacgggc ccaccctgcg cccgctggcc ctgctcgacg gcgcggcgct
    10561 caccaccctg cgcaccccgg cggtctccgc cctcgcgctg cggtacctgg ccccggccgg
    10621 gcgcccgttg cggctggtgc tcttcggctc cgggccgcag gcgtacgcgc atctggaagc
    10681 ggtactcgcc gtacgggagt tggccgaggt cgtggtggtg gcccggcacc ccgggcgggc
    10741 ggaggggctc gccgcgtacg cgcgtaccct gggcccggcc gcccggaccg gcactccgaa
    10801 ggaagtggcc gatgccgatc tggtgatctg ctgcaccacg gcccgggaac ccctcttcga
    10861 cgggcgcctc gtcgggccgg gcgccaccgt cgtcgccgtc ggctcgcacg agccgacggc
    10921 ccgtgaaacg gacaccgccc ttgtgcgacg ggcggcggtg tacgtggagt cccgggcggc
    10981 ggccctgcgc gaggccgggg acctcctggt cccggaggcc gagggggcca tcgggcccgg
    11041 tcatatcacc ggcaccctcg ccgacctggt gggcggccgg atgccggcgg gcggaccgct
    11101 gagttgtcca cagctcttca agagtgtggg catggcctgg gaagatctcg ctgtggcggt
    11161 cgcgctggtc gaggccgccg gagtgtgagc agcgcaacgt gacattgtac gctgttcctc
    11221 tcctcgtcgg tggtgtacgg gggtctcgga ggaaatgcaa tgggtgacct gaagcagcac
    11281 agtctcatca aggcccagga acgcctccgc gaccaggtcg gccacgcact ccgagccgcg
    11341 ctgatagcgg gtgagctgcg ccccgggagc gtctactccg cacccggcct cgcggccgaa
    11401 ctcggcgtct cggccacacc ggtgcgcgag gccatgctcg acctggcccg ggaggggctc
    11461 gtcgagcccg tccgcaacaa gggcttccgg atcaccgagg tcagtgagcg cgacctggac
    11521 cagttcaccg aactgcgcac gatgatcgag gtgccgacca tcggccggat cacgaagatc
    11581 gccacggccg agcagctgga ggcgctgcgc cccatcgccg aggagatcgt cagcagcgcg
    11641 cgcgagcaca acctcatcgg ctacctggag gccgaccgcc gcttccacct gtccctgctg
    11701 gccctcgcgg gcaacgaccg gctggtggag acggtcggcg acctgcgcaa gcggtccagg
    11761 ctgtacggcc tgaccggcct ggacgaggcc ggcaagctgg tctcctcggc cgaggagcac
    11821 atcgaactgc tcgacctgat gatcggcggc gacgccgaag cggcgcagga gtgcatgctc
    11881 cgccacctcg gccacgtccg ctccctgtgg gcccagggcc gcgacgaacc ggtcggccgc
    11941 gtccccggtg gcctcggctc ggggctgcag tagcagcccg cctcaacacg gtgaacggcc
    12001 gccgccggac tgccgcggcc ggtccgccga gatgatcgcc gcctccgggc gcggtacggg
    12061 ggtgccgtcg ggcaggagca ggaccggcgg gtccacgacg gcgcccacgt cggccgccgt
    12121 gctgctcgcg ttcaccgccg tgacccgccc cgtgggctgc ggggtcagca ccgacgtgtc
    12181 ccgcacgtac gccacgaaac tctcgaagtc gcgctggtgc c
//
