LOCUS       NZ_BMVL01000003        10405 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces avidinii strain JCM 4726 sequence03, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVL01000003
VERSION     NZ_BMVL01000003.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces avidinii
  ORGANISM  Streptomyces avidinii
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 663611)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650695.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,659
            CDSs (total)                      :: 7,582
            Genes (coding)                    :: 7,337
            CDSs (with protein)               :: 7,337
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 80 of 245
            Pseudo Genes (incomplete)         :: 200 of 245
            Pseudo Genes (internal stop)      :: 10 of 245
            Pseudo Genes (multiple problems)  :: 43 of 245
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:19:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 630149
            Orig. end                         :: 640554
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVL01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(181..195)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            467..2062
                     /locus_tag="IE257_RS10975"
                     /old_locus_tag="GCM10010343_22620"
     CDS             467..2062
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037768.1"
                     /locus_tag="IE257_RS10975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22620"
                     /product="hypothetical protein"
                     /protein_id="WP_189967215.1"
                     /transl_table=11
                     /translation="MPSSRIRRAWLSAAAAAALAFTGLAGPATAKDLPPDEPGSWIQEP
                     RATEGALPAFGTRAAAVPGGAASPGDFTLSPGEMNAETTERVSRLASVLPESGVQDLLA
                     AANRPIEPGCSRDPFAAAPDPAVKYCLKDDDSVSREWVPQGFTGVSDAKANEFWGAAGK
                     IQLFASYDGWDPGRESDPNPAAGDCTAAELEDNDACNQKGVRITFVQSRTNPATGSPEI
                     KYRHVLLGWTYVNSADHVSFDGLHAGEYPIQKGVHAGGIVWYGNYLYVADTRGGLRVFD
                     MRLIMDLDPDNDPSTHDPMGTDSDGVKTTADVEDKTKVGRHDNVWYSFGYRYVMPQVAA
                     WKFKAAQSNPKGSYACVSTGAPKASYISLDRSTVPDRLVMGEYCRPESGYPSTGRIASY
                     PVAALEGRSSDVTAQGWANYLPLANGGAQGAAAYKGTLYVNESKGTDEPGNLWRYQWSD
                     GRLVQNGQAVKTARGAEDLYVERGTGRLWSLSEHRPGSADDCRANPGPGDPAGADFCQR
                     VLYAHDLSRLDSRP"
     gene            complement(2120..2671)
                     /locus_tag="IE257_RS10980"
                     /old_locus_tag="GCM10010343_22630"
     CDS             complement(2120..2671)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382145.1"
                     /locus_tag="IE257_RS10980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22630"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_189967217.1"
                     /transl_table=11
                     /translation="MSEQRRPADPSPAADEVDEVTHAVLAASQLLASIGSRALAAAGYG
                     GRDGHGVTPAQFRMLEVLVAHGHVKLVALAGAMAVNPSTAMRMVDRLIAAGLADRRPNR
                     ENRRETSLTATDSGLRLVAAVTARRRAETAAALARLGPAERAVLVGALGAFTAAGAQPA
                     EGGPHAFPDLLGCAGPYVLG"
     misc_feature    2789..2809
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.54"
                     /tool="antismash"
     misc_feature    2809..2826
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.88"
                     /tool="antismash"
     misc_feature    complement(2823..2836)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.17"
                     /tool="antismash"
     misc_feature    complement(2824..2837)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.82"
                     /tool="antismash"
     misc_feature    2824..2838
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.27"
                     /tool="antismash"
     misc_feature    2825..2839
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.2"
                     /tool="antismash"
     misc_feature    complement(2848..2861)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.71"
                     /tool="antismash"
     gene            2992..3552
                     /gene="ectA"
                     /locus_tag="IE257_RS10985"
                     /old_locus_tag="GCM10010343_22640"
     CDS             2992..3552
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987798.1"
                     /locus_tag="IE257_RS10985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22640"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_189967219.1"
                     /transl_table=11
                     /translation="MTGAHADLATRPRTAAPTTNPPGIRADRPDLCDGPDLWRIARDAK
                     TLDVNSPYSYLLWCRDFAATSAVARDGDGRPVGFVTGYLRPDTPATLLIWQVAVDADAR
                     GQGIAGYLLDHLTARVAAEHVLDALETTISPGNTASERLFASYAERHGAHLAREVLFAE
                     EHFPGGAGHHDAEVLHRIAPLAR"
     gene            3658..4926
                     /gene="ectB"
                     /locus_tag="IE257_RS10990"
                     /old_locus_tag="GCM10010343_22650"
     CDS             3658..4926
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 1.6e-72.
                     Score: 236.3. Matches aSDomain:
                     nrpspksdomains_IE257_RS10990_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180700.1"
                     /locus_tag="IE257_RS10990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22650"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_189967223.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-79, bitscore:
                     262.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTAPVPTVFDTVESEVRSYCRAWPVVFDRAEGSRLYDENGRSY
                     LDFFAGAGSLNYGHNNPVLKQALLDYIARDGITHALDMSTTAKRAFLETFHSTVLAPRA
                     LPYKVMFPGPTGTNAVEAALKLARKAKGREAVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHSTPMPFDNYLGGRIPDFLWFERLLEDSGSGLNDPAAVIVETVQGEGGVNVARA
                     EWLRALADLCRRRDMLLIVDDVQMGCGRTGDFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALQTYWRDGVLRERTRVQGNRIERALV
                     DLCADHERDGASYRGRGMVWGIEFTRKERAAEVCHRAFEHGLLVETSGPESEVVKLLPP
                     LTVSADELDEGLDILARSVRETG"
     aSDomain        3742..4752
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS10990_Aminotran_3.1"
                     /evalue="1.60E-72"
                     /label="IE257_RS10990_Aminotran_3.1"
                     /locus_tag="IE257_RS10990"
                     /protein_end="365"
                     /protein_start="28"
                     /score="236.3"
                     /tool="antismash"
                     /translation="FDRAEGSRLYDENGRSYLDFFAGAGSLNYGHNNPVLKQALLDYIA
                     RDGITHALDMSTTAKRAFLETFHSTVLAPRALPYKVMFPGPTGTNAVEAALKLARKAKG
                     REAVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHSTPMPFDNYLGGRIPDFLWFER
                     LLEDSGSGLNDPAAVIVETVQGEGGVNVARAEWLRALADLCRRRDMLLIVDDVQMGCGR
                     TGDFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATAALQTYWRDGVLRERTRVQGNRIERALVDLCADHERDGASYRGRGMVWGIEFT"
     gene            5001..5405
                     /locus_tag="IE257_RS10995"
                     /old_locus_tag="GCM10010343_22660"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="IE257_RS10995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22660"
                     /product="ectoine synthase"
                     /protein_id="WP_189967225.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.9e-53, bitscore:
                     172.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFQDIEGTDRHVRAASGTWESKRVILAKERVGFSLHETVLY
                     AGTETSMWYANHVEAVVCVEGEAELTDEETGERHTITPGTVYLLDGHERHTLRVVREFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPDPV"
     gene            5455..6357
                     /gene="thpD"
                     /locus_tag="IE257_RS11000"
                     /old_locus_tag="GCM10010343_22670"
     CDS             5455..6357
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848585.1"
                     /locus_tag="IE257_RS11000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22670"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_189967227.1"
                     /sec_met_domain="PhyH (E-value: 1.1e-38, bitscore: 128.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVSERTLADLYPTRGSEEVLIGRKDPVVWSEPGTPGPFWAREL
                     EDFERDGFLTAEQLIGPEEVEEYRAEMDRLVSDPAVRADERAIVEPGSGEVRSVFEVHR
                     ISEVFARLAADPRLADRARQILGSDVYVHQSRINAKPGFGASGFYWHSDFETWHAEDGL
                     PRMRTVSVSIALTPNHTTNGSLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPTPE
                     ALTRFASACGIRQFTGAAGSATWFDCNAMHGSGDNITPYPRSNVFLVFNSVENLPAEPF
                     AAPVRRPSFIASREFTPMR"
     gene            6464..7951
                     /locus_tag="IE257_RS11005"
                     /old_locus_tag="GCM10010343_22680"
     CDS             6464..7951
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309675.1"
                     /locus_tag="IE257_RS11005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22680"
                     /product="MFS transporter"
                     /protein_id="WP_229920256.1"
                     /transl_table=11
                     /translation="MNLGRTKNEPGTPPRQGAALAALAAAQFTVMLATSIVNVALPQIR
                     SGVGLSDGGTTWVVNAYGLAFGALLLAGGRAGDLLGRRRVLIASLAVFAGASLAAGLAG
                     SPGLLIAARAVQGIGAAAIAPAALALAMDLFPPGPGRGRVLGVWGAVSGAGGAGGVLLG
                     GVLTQAWGWPWLFHAVALGTTLVLIAVAALVPRAGTRGSGRFDLLGTATVTLALTCLVW
                     ALTGARAQGWTDAGVLAALLGAAVLLMVFVLVERRRPDALVPARLFTAGRVAAGNLLMA
                     LLGSVWIALFFFLPLYQQQVLGMSPLATGAGQLPLAVANMLGAILAPRIAARIGTTATL
                     TTGLLAEAAGLLWLSRMRADGSYLVDALGPSVLVGLGLSLAFVQLTALAVDGVPRQDTG
                     LAGGLVNTTRQVGGAIGLAALATLAGSVTARAATHQPHLEALTAGYRAAFAVSAAVLAA
                     TALLAVPLTRPLRCSRRVAAPATPSPQDRPAEHASAT"
     gene            7997..8446
                     /locus_tag="IE257_RS11010"
                     /old_locus_tag="GCM10010343_22690"
     CDS             7997..8446
                     /NRPS_PKS="Domain: Polyketide_cyc2 (11-146). E-value:
                     7.3e-23. Score: 73.5. Matches aSDomain:
                     nrpspksdomains_IE257_RS11010_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022072.5"
                     /locus_tag="IE257_RS11010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22690"
                     /product="SRPBCC family protein"
                     /protein_id="WP_189967229.1"
                     /transl_table=11
                     /translation="MHTTIDTTAPVVVRLSTAIDAPPATVWELHTDIDNWAAWNDGVDR
                     ARLDGPLEVGSRFTWLTHGLRITSTLLELVPGQRIVWGGTVDGILGIHVWTFEASGAGA
                     GVTVHTEESWSGDPVDAAVDELTIALRSSLQSWLDSLKARAEQTA"
     aSDomain        8030..8434
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE257_RS11010_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="7.30E-23"
                     /label="IE257_RS11010_Polyketide_cyc2.1"
                     /locus_tag="IE257_RS11010"
                     /protein_end="146"
                     /protein_start="11"
                     /score="73.5"
                     /tool="antismash"
                     /translation="VVRLSTAIDAPPATVWELHTDIDNWAAWNDGVDRARLDGPLEVGS
                     RFTWLTHGLRITSTLLELVPGQRIVWGGTVDGILGIHVWTFEASGAGAGVTVHTEESWS
                     GDPVDAAVDELTIALRSSLQSWLDSLKARAE"
     gene            8494..9099
                     /locus_tag="IE257_RS11015"
                     /old_locus_tag="GCM10010343_22700"
     CDS             8494..9099
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061141.1"
                     /locus_tag="IE257_RS11015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22700"
                     /product="ABC transporter permease"
                     /protein_id="WP_189967231.1"
                     /transl_table=11
                     /translation="MSSHTTLSPPVVQDHPTRRPREVDNRAAYVSFGLAYVLGHGAAAA
                     SHGAAPLLDLPGWLPTALLGLGLVTGIALATAAALRAQHGATAPEVLSGKLLGAAWITA
                     FTALFLAITGLTSVLDHPELQSVLWPAGSGLVVGLLYLAEGAVRRNVLHYSLGVWLAIT
                     STAALALAAPAFYWVLAVGGGGAYVLAAALEHRRLHAL"
     gene            complement(9106..9711)
                     /locus_tag="IE257_RS11020"
                     /old_locus_tag="GCM10010343_22710"
     CDS             complement(9106..9711)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735601.1"
                     /locus_tag="IE257_RS11020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010343_22710"
                     /product="hypothetical protein"
                     /protein_id="WP_189967233.1"
                     /transl_table=11
                     /translation="MRLYAQTPARRNRQVLADLIAVGLMAAAVWFALTVHDAIMMLAEP
                     GRRVESSGDSLASGLSDAGDAASNVPFVGDLLKKPLRSAADASTGIADAGQSMQDAVGQ
                     LATLTALALIVLPVAVVLLLWLPARLRWIRRSITLRRLFDAPGGADLLALRALTGPLGD
                     LAALPTPAGGLADAWRRGDERVIGELSEVALRRAGLRP"
     misc_feature    complement(10042..10056)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.93"
                     /tool="antismash"
     misc_feature    complement(10324..10336)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
ORIGIN
        1 tggcctcgct cccgcggtac gcgggcgtct tcggcctccg ggggatgacg cgggccgggg
       61 aggtccagaa cgccatgcac gaggcccatc cgcggacgcc gcactggtac ctgccctccg
      121 tggggacgga ccgggaactc cagggcatgg gagtcggttc ggcgctgttg cgtcagcaac
      181 tggccgactg cgaccggttc gggcaatccg tctacctgga gtccagcaag gacaccaacg
      241 tccccttcta cgaagggctg ggcttccgcg tcaccggcga gatccggatt cccgggggcg
      301 gaccgaccct ctggcccatg tggcgcgacc cccgccgttg agggttcccg tcaactgtcc
      361 tgctcagcag gtttgttgac gaaccgatcg cccttgcgtt ccgcgcaact gttctggaaa
      421 cctccgcttg actcacctgt cgcacgagca acggagcaac ccccgcatgc cctcaagtcg
      481 aatacgtcgt gcctggctct cggccgccgc cgcggcagcg ctcgcgttca ccggtctcgc
      541 aggaccggcg acggcgaagg acctgccgcc cgatgagccg ggctcctgga tccaggagcc
      601 ccgcgccacc gagggcgccc tcccggcctt cggtacccgc gccgctgccg tgcccggtgg
      661 cgccgcctcg ccgggcgact tcacgctgtc gcccggcgag atgaacgccg agacgaccga
      721 gcgggtctcc cggctggcct cggtcctgcc cgagtcgggc gtgcaggacc tgctggccgc
      781 ggcgaaccgc cccatcgagc cggggtgttc ccgcgacccg ttcgccgccg ccccggaccc
      841 cgccgtcaag tactgcctga aggacgacga ctccgtctcg cgggagtggg tcccgcaggg
      901 cttcaccggc gtctccgacg ccaaggccaa cgagttctgg ggcgcagccg ggaagatcca
      961 gctgttcgcc tcctacgacg gctgggaccc gggccgggag agcgacccga acccggcggc
     1021 cggcgactgc accgcggccg agctggagga caacgacgcc tgcaaccaga agggcgtccg
     1081 gatcaccttc gtgcagagcc ggaccaaccc ggccaccggt agtcccgaga tcaagtaccg
     1141 gcacgtcctg ctgggctgga cctacgtgaa ctccgccgac cacgtctcgt tcgacggcct
     1201 gcacgccggc gagtacccga tccagaaggg cgtccacgcc ggcgggatcg tctggtacgg
     1261 caactacctc tacgtcgccg acacgcgggg cggcctgcgc gtcttcgaca tgcgcctcat
     1321 catggacctg gacccggaca acgaccccag tacgcacgac ccgatgggca ccgactccga
     1381 cggagtgaag accaccgccg acgtcgagga caagacgaag gtcggccggc acgacaacgt
     1441 ctggtacagc ttcggctacc gctacgtcat gccgcaggtg gcggcgtgga agttcaaggc
     1501 ggcgcagagc aaccccaagg gttcctacgc ctgtgtctcc accggcgccc cgaaggcgtc
     1561 ctacatctcc ctggaccgca gtacggtccc ggaccgcctc gtcatgggcg agtactgccg
     1621 tccggagagc ggctacccct ccacgggccg tatcgcctcg tacccggtag cggccctgga
     1681 ggggcgctcc tcggatgtga ccgcccaggg ctgggccaac tacctgcccc tggccaacgg
     1741 cggcgcccag ggcgcggcgg cgtacaaggg aacgctgtac gtgaacgagt ccaagggcac
     1801 ggacgagccg ggcaacctct ggcgctatca atggagtgac ggccggctcg tccagaacgg
     1861 ccaggccgtc aagaccgccc ggggcgccga ggacctgtac gtcgagcgcg gcacgggacg
     1921 cctgtggtcc ctctccgaac accgtccggg ctcggcggac gactgccggg cgaatccggg
     1981 ccccggagat ccggccggcg cggacttctg ccagcgggtg ctctacgccc atgacctgag
     2041 ccggctcgac agccggccgt agccccggtc gcagccgtag gcgcaacgga ggcgggccgc
     2101 ggccgagggg cggcccgcct cagcccagga cgtacggccc cgcgcacccg aggaggtccg
     2161 ggaaggcgtg gggtccgccc tcggcgggct gcgcacccgc ggcggtgaac gcgccgaggg
     2221 cgccgaccag tacggcgcgt tcggcgggcc cgagccgcgc gagggcggcg gccgtctccg
     2281 ccctgcgccg ggccgtcacc gcggccacca gccgcaggcc ggagtccgtg gcggtgagcg
     2341 acgtctcccg ccggttctcc cggttgggcc tgcggtccgc caggcccgcg gcgatcaacc
     2401 ggtcgaccat gcgcatcgcg gtcgacgggt tgaccgccat cgcccccgcg agggcgacca
     2461 gtttcacgtg cccgtgggcc accaggacct cgagcatgcg gaactgcgcg ggcgtgaccc
     2521 cgtggccgtc gcggcccccg tacccggcgg ccgccagggc ccgggacccg atggacgcga
     2581 gcagttggga agcggccagg accgcgtgcg tcacctcgtc gacctcgtcc gcggcgggtg
     2641 acggatccgc gggtcgccgt tgctcgctca tacccctttg taccgtccga ccgtgcggga
     2701 cacccgacgg gccgaaaggg cgactcgcct ttcccgttct tccgatccga aacgggggaa
     2761 caggggaatg tcgtggcttt cacaccgtaa atatggcaca ggacaaagac gttatagttc
     2821 cgttatcttt ccaaaatttg ggatggcttg tcctgataaa tgcaggtttt ctccgtgggg
     2881 gagggtgggc ccgactcgcc tcggcgccgc tgccggataa cacgaacgca cggcaggcgg
     2941 aaccccgttc gcgcatgtct tttcagccga tgatcggtag cttcggcccg catgaccggt
     3001 gctcatgcag acctggccac ccgcccccga accgccgccc ccacgacgaa cccacccgga
     3061 atccgggccg accgccccga cctgtgcgac ggccccgacc tctggcgcat cgcccgcgat
     3121 gcgaagacgc tggacgtcaa ctcgccctac agctacctgc tctggtgccg cgacttcgcc
     3181 gcgacctccg ccgtcgcccg cgacggcgac ggtcggcccg tcggcttcgt caccgggtac
     3241 ctgcggcccg acaccccggc caccctgctg atctggcagg tcgccgtcga cgccgacgcc
     3301 cgcgggcagg ggatcgccgg gtacctcctg gaccacctca ccgcacgcgt cgccgccgag
     3361 cacgtgctcg acgcgctgga gaccaccatc agcccgggga acaccgcctc ggagcggctc
     3421 ttcgcgtcct acgccgaacg ccacggcgcc cacctcgccc gggaagtcct cttcgccgag
     3481 gagcacttcc ccggcggcgc cggacaccac gacgccgagg tgctccaccg catcgcgccc
     3541 ctggcccgct gaccaccgcg agctccgccg aacgccgaac gccgaacgcc gaacgccgaa
     3601 cgccgaacac cacgtccacc accgtccgtc tccggacacc gaggagaggc agcccccttg
     3661 accacgacag cacccgtgcc gaccgtcttc gacaccgtcg agtcggaggt gcgcagctac
     3721 tgccgcgcct ggcccgtcgt cttcgaccgc gcggaaggaa gccgcctcta cgacgagaac
     3781 ggccgctcct acctggactt cttcgcaggc gccggctccc tcaactacgg gcacaacaac
     3841 cccgtgctca aacaggcgct gctcgactac atcgcgcgcg acggcatcac gcacgccctg
     3901 gacatgtcga cgaccgccaa gcgggccttc ctggagacct tccactccac cgtcctcgcc
     3961 ccgcgcgcac tgccgtacaa ggtgatgttc ccgggcccca ccggcaccaa cgctgtcgag
     4021 gcggcgctga agctggcgcg caaggccaag ggacgcgagg cggtcgtctc cttcaccaac
     4081 gccttccacg ggatgtccct gggctcgctc gccgtcaccg gcaacgcctt caagcgcgcg
     4141 ggcgccggca taccgctggt ccacagcacg cccatgccct tcgacaacta cctcggtggc
     4201 cggatcccgg acttcctctg gttcgagcgg ctcctggagg acagcgggtc cggcctgaac
     4261 gacccggccg ccgtgatcgt cgagaccgta cagggcgagg gcggggtcaa cgtggcgcgc
     4321 gcggagtggc tgcgtgccct cgccgatctg tgccgccgcc gcgacatgct gctgatcgtc
     4381 gacgacgtcc agatgggctg cggccgcacg ggtgacttct tctccttcga ggaggcgggc
     4441 atcacacccg acatcgtcac cgtctcgaag tccatcagcg gctacggact gcccatgtcg
     4501 ctctgcctgt tcaagccgga gctcgacatc tgggagccgg gcgagcacaa cggcacgttc
     4561 cgaggcaaca accccgcctt cgtcacggcg accgccgcac tccagacgta ctggcgcgac
     4621 ggtgtcctgc gcgaacgcac ccgtgtccag ggcaaccgca tcgagcgggc gctcgtcgac
     4681 ctgtgcgccg accacgagcg ggacggcgcc tcgtaccgcg gccgcggcat ggtgtggggc
     4741 atcgagttca cccgcaagga gcgcgccgcc gaggtgtgcc accgggcctt cgagcacggc
     4801 ctgctcgtcg agacctccgg gcccgagagc gaggtggtca agctgctgcc gccgctcacc
     4861 gtgtcggcgg acgagctcga cgagggcctc gacatcctcg cgcgctccgt ccgcgagacg
     4921 ggctgacccg ccgcccccac cccacccgcc accaccgggc cccgacagga acgccccgca
     4981 ccacgaaagg cagcaccacc gtgatcgtcc gatccttcca ggacatcgag ggcaccgacc
     5041 gccacgtcag ggcggcgtcg ggcacctggg agagcaagcg cgtcatcctg gcgaaggaac
     5101 gcgtcggctt ctccctccac gagaccgtcc tgtacgccgg caccgagacg tccatgtggt
     5161 acgcgaacca cgtcgaggcg gtcgtgtgcg tcgagggcga ggccgaactc accgacgagg
     5221 agaccggcga gaggcacacc atcacgcccg gcaccgtcta cctgctcgac ggccacgagc
     5281 gccacaccct gcgcgtcgtc cgcgagttcc gctgcctgtg cgtcttcaac ccgccggtca
     5341 ccggccgcga ggaccacgac gagaacggcg tgtacccgct gctcaccgaa ccggacccgg
     5401 tctgaccgac tccgcagcaa cccaccgaca ccgagaggag aggcaagcac caccatgacc
     5461 actgtctccg aacgcaccct cgccgacctt taccccaccc ggggctccga agaagtcctc
     5521 atcggccgca aggaccccgt cgtgtggtcc gaacccggca cccccggacc gttctgggcg
     5581 cgcgagctcg aggacttcga acgcgacggt ttcctcaccg ccgagcagct gatcggcccg
     5641 gaggaggtcg aggagtaccg ggccgagatg gaccggctgg tgagcgaccc cgccgtccgg
     5701 gccgacgaac gggccatcgt ggaaccagga tcgggcgagg tccgctcggt cttcgaggtc
     5761 caccgcatca gcgaggtctt cgcacggctc gccgccgacc cgcggctcgc cgaccgggcc
     5821 cggcagatcc tcggttccga cgtgtacgtc caccagtccc gcatcaacgc caagcccggc
     5881 ttcggcgcga gcggcttcta ctggcactcc gacttcgaga cctggcacgc cgaggacggc
     5941 ctgccccgca tgaggaccgt ctcggtgtcc atcgccctca cgccgaacca cacgaccaac
     6001 ggcagcctga tgatcatgcc gggttcgcac cggacgttcc tcggttgtgc gggcgcgacg
     6061 ccgaaggaca actacaagaa gtcgctgcag atgcaggacg ccggaacgcc cacccccgag
     6121 gccctgaccc ggttcgcctc cgcctgcggc atccggcagt tcaccggagc cgccggatcg
     6181 gccacctggt tcgactgcaa cgcgatgcac ggttccgggg acaacatcac gccctacccc
     6241 cgcagcaacg tcttcctggt gttcaacagc gtggagaacc tgcccgcgga gcccttcgcg
     6301 gccccggtcc ggcgcccgtc cttcatcgcc tcgcgggagt tcaccccgat gcgctgacgg
     6361 acgcgcgcgg catggccgag tggacagggg cggacacgac tggacagtga ccgcccctgt
     6421 cgggcatgga atgtggacgc cgtccgaccc gaggctgtgc gccatgaacc tcggacgcac
     6481 gaagaacgaa cccggaaccc caccccgcca gggagccgcg ctcgcggcgc tggccgcagc
     6541 gcagttcacc gtcatgctcg ccacctcgat cgtgaacgtg gccctgccgc agatccgttc
     6601 aggggtgggc ctgtcggacg gcggcaccac ctgggtggtc aacgcctacg gcctggcgtt
     6661 cggggcgctg ctcctggccg gcggccgggc aggcgatctg ctcggccgcc gccgtgtgct
     6721 catcgccagc ctcgcggtgt tcgcgggagc ctcgctggcg gccggcctgg ccggctcgcc
     6781 gggcctgctg atcgccgccc gggccgtgca gggcatcggc gccgccgcca tcgcacccgc
     6841 cgcactcgcc ctggcgatgg acctgttccc gcccggcccc ggacggggcc gggttctcgg
     6901 ggtgtggggc gccgtctccg gcgcgggcgg agcggggggc gtcctgctgg gcggggtact
     6961 cacccaggca tggggctggc cctggctctt ccacgccgtc gcgctcggta cgacgctggt
     7021 cctcatcgcc gtggcggcgc tcgtgccccg ggccggcacc cgcgggtccg ggcggttcga
     7081 cctgctgggt accgcgaccg tcaccctcgc cctgacctgc ctggtgtggg cactgaccgg
     7141 cgcccgcgcc cagggctgga ccgacgccgg ggtgctggcc gccctcctgg gcgccgccgt
     7201 gctgctcatg gtcttcgtcc tcgtcgaacg ccgccgaccg gacgccctgg tcccggccag
     7261 gctgttcacg gccggccgcg tcgccgcggg caatctgctg atggcgctgc tggggtccgt
     7321 atggatcgcg ctgttcttct tcctcccgct ctaccagcaa caggtcctcg gcatgagccc
     7381 gctggccacc ggcgccggcc aactcccgct ggccgtggcc aacatgctcg gcgcgatcct
     7441 cgcgccccga atcgccgccc gcatcggcac cacggcgacc ctgaccacgg gcctgctcgc
     7501 cgaggccgcc gggctgctgt ggctgtcacg gatgagagcg gacgggagct atctcgtcga
     7561 cgccctcggc ccgagcgtcc tggtcggcct gggcctgagc ctcgccttcg tccagctcac
     7621 cgcgctcgcg gtggacggcg tcccgcggca ggacacgggc ctggctggcg gactggtgaa
     7681 caccacccgc caggtcgggg gcgcgatcgg tctcgcagcc ctcgccaccc tggccggctc
     7741 cgtcaccgcc cgcgccgcca cgcaccagcc ccacctggaa gccctcaccg cgggctaccg
     7801 ggccgccttc gcggtctccg ccgcggtcct ggccgccacc gcactcctcg cggtgccgct
     7861 cacccgcccg ctgcgctgct cccgccgcgt cgccgcgcct gccaccccct ccccacagga
     7921 ccggccggcc gaacacgcct ccgccacctg atcccgtacc cgatccgaca acccgacgac
     7981 cgacaaggac acatccatgc acaccaccat cgacaccacg gcccccgtcg tcgtccgcct
     8041 ctcgacggcg atcgacgccc ctcccgccac cgtctgggag ctgcacaccg acatcgacaa
     8101 ctgggcggcc tggaacgacg gcgtcgaccg ggcccggctg gacggcccgc tggaggtcgg
     8161 cagccgcttc acctggctca cccacggact gagaatcacc tccacgctcc tcgaactggt
     8221 ccccggacag cggatcgtct ggggcgggac ggtcgacggc atcctcggca tccacgtgtg
     8281 gaccttcgaa gcgtccggcg ccggtgcagg tgtcaccgtc cacaccgagg agtcgtggag
     8341 cggagacccc gtcgacgccg cggtcgacga actcaccatc gccctgcgga gctccctgca
     8401 gagctggctc gacagcctca aggcacgcgc cgaacagacc gcctgaccgt cagccccgcc
     8461 cccaccacgc cgcccccgat ggaggtttcc cccgtgtcca gccacaccac cctctcgccg
     8521 cccgtcgtcc aggaccaccc cacccgccgc ccgcgcgagg tcgacaaccg cgcggcctac
     8581 gtcagcttcg ggctcgccta cgtactcggc cacggcgcgg ccgcggcctc ccacggcgcc
     8641 gcacccctgc tcgacctgcc cggctggctg ccgacggccc tcctcggcct cgggctcgtg
     8701 accggcatcg ccctggccac cgccgccgcc ctgcgggccc agcacggggc caccgcaccg
     8761 gaggtcctct ccggcaagct gctgggtgcc gcctggatca ccgccttcac cgcgctgttc
     8821 ctcgccatca ccggcctgac ctccgtgctc gaccatccgg aactccagtc cgtcctgtgg
     8881 cccgccggct ccggactcgt cgtcggcctg ctctacctcg cggaaggcgc cgtacgccgc
     8941 aacgtgctgc actacagcct cggcgtctgg ctcgccatca cctccaccgc ggccctcgcg
     9001 ctcgccgccc ccgcgttcta ctgggtcctc gccgtcggcg gtggcggcgc ctacgtcctc
     9061 gccgcggccc tcgaacaccg ccgcctccac gccctctgac ggccgtcagg gccgcaggcc
     9121 ggcccgccgg agggcgacct cggacagctc gccgatgacg cgctcgtccc cgcgccgcca
     9181 ggcgtcggcg agaccgcccg caggggtggg gagcgcggcg aggtcgccca gcggtccggt
     9241 cagggcacgc agggcgagca ggtcggcgcc gcccggcgcg tcgaacaggc gccgcagggt
     9301 gatgctgcgc cggatccagc gcaggcgcgc ggggagccac agcagcagga cgacggccac
     9361 cgggaggacg atcagggcga gcgcggtcag cgtggccagc tggccgacgg catcctgcat
     9421 cgactgtccg gcatcggcga tgccggtact ggcgtcggcc gccgaccgga gcggcttctt
     9481 caggaggtcg ccgacgaacg gcacgttcga ggcggcgtcg ccggcgtcgg agagtccgga
     9541 ggcgaggctg tcgcccgagc tctcgaccct ccgccccggc tcggccagca tcatgatcgc
     9601 gtcgtggacg gtcagggcga accacaccgc ggcggccatg aggcccacgg cgatcaggtc
     9661 cgcgagtacc tggcggttcc gacgtgcggg ggtctgtgcg tagaggcgca tcggcgggct
     9721 cccgttcgag tcaggacgtc gaggaggtac gggcccctcg ggcgggcccg gccggtcggc
     9781 agaccgtacg ccgggtctct acccttccac cggcccctgt ggacgaagcg cgcactgccg
     9841 gaaccgggcg ggcgggccgg ccgacggccc gcccgcccgc ccgtcgggcc cggcaagatc
     9901 cggaagagac ggcctaggcg aggtcgaacc ggtcgaggtt catcaccttg tcccaggcgg
     9961 agacgaagtc acggacgaac ttctctcccg cgtccttcga cgcgtagacc tcggagagcg
    10021 cccggagctg ggcgtgcgaa ccgaagacga ggtccaccgc ggtcgccgtc cacctgacct
    10081 cgcccgtggc gcggtcccgg ccctcgaaca cgttctccgc cgactcggac gccttccact
    10141 gtgtacccat gtcgagcagg ttgacgaaga agtcgttcgt caacgactcc gggcggtccg
    10201 tgaacacgcc gtgcggggac tgccggaagc cggtgttcat ggcccgcatg ccgccgacga
    10261 gcaccgtcat ctcgggggcg gtcaggttca gcatgccggc gcggtccagc agaagcgttt
    10321 ccggtgacag cttctcgccc gcccgcaggt agttccggaa cccgtccgcc tcgggttcga
    10381 ggacggcgaa ggagtccacg tcggt
//
