LOCUS       NZ_BMVE01000036         2954 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces goshikiensis strain JCM 4640 sequence36, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVE01000036
VERSION     NZ_BMVE01000036.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces goshikiensis
  ORGANISM  Streptomyces goshikiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650555.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:30:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,491
            CDSs (total)                      :: 7,410
            Genes (coding)                    :: 7,286
            CDSs (with protein)               :: 7,286
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 44 of 125
            Pseudo Genes (incomplete)         :: 101 of 125
            Pseudo Genes (internal stop)      :: 9 of 125
            Pseudo Genes (multiple problems)  :: 27 of 125
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:38:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2954
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVE01000036.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2954
                     /culture_collection="JCM:4640"
                     /db_xref="taxon:1942"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces goshikiensis"
                     /strain="JCM 4640"
                     /submitter_seqid="sequence36"
                     /type_material="type strain of Streptomyces goshikiensis"
     region          1..2954
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..2954
                     /SMILES="C(C)C(O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2954
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[8:>2954](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      9..>2954
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            9..>2954
                     /locus_tag="IE248_RS37015"
     CDS             9..>2954
                     /NRPS_PKS="Domain: PKS_AT (7-254). E-value: 3.8e-85. Score:
                     277.8. Matches aSDomain:
                     nrpspksdomains_IE248_RS37015_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (545-719). E-value: 5.1e-58.
                     Score: 187.9. Matches aSDomain:
                     nrpspksdomains_IE248_RS37015_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (816-899). E-value: 7.6e-29.
                     Score: 91.9. Matches aSDomain:
                     nrpspksdomains_IE248_RS37015_PKS_PP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012901.5"
                     /locus_tag="IE248_RS37015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_268254124.1"
                     /sec_met_domain="PKS_AT (E-value: 6.4e-87, bitscore: 277.8,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 7.5e-61, bitscore:
                     191.3, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2.7e-09,
                     bitscore: 22.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.9e-15, bitscore:
                     43.5, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 2.6e-23, bitscore: 68.6,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 8.3e-16, bitscore:
                     44.4, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQVLDDEAALERVEVLQPASFAVMVSLAALWRSLGVVPDAVVGH
                     SQGEIAAAVVAGGLSLEDGARVVVLRARLIGRELAGHGGMASVALPVTVVEERLERWAG
                     RLGVAVVNGPSATVVAGDTDAVAEFVTVCEAEGVRARRLPVDYASHSAHVETLAAELEE
                     ILADVRPVSGEIPFYSTVEAAAIDTASLDAGYWFGNLRRRVRFQETVEQLLADGFPVFV
                     EASAHPVLTAAVQETAESVGRQVCAVGSLRRDEGGLRRFLTSVAEAFVQGVDVTWPALF
                     DDTGARTIDLPTYPFQHQRYWLESRPSAAVVPSGVQDGLSYEVTWKSLSLPESVRLDGR
                     WLLVVPEDLDADGTRVAHDVEQALTTHGATVSRLTVDVTATDRTDLSARLTTTAAEEQE
                     PLGGILSLLGWAEGAQVDRPTVPIAIATSLALVQAVGDAGFGVPVWAVTRGAVSVVPGE
                     VPETAGAQLWALGRVAALELPDRWGGLIDLPADTDTRTAALALRALAAGIVAGEDQLAV
                     RPSGAYGRRVVQAGHRKPKGAKPEWRPRGTVLVTGGMGAIGTRVARWLARNGAEHLVLT
                     GRRGAGTPGADELAGELRASGVQVTLAACDVSDRAALAALIDAHPPTAVFHTAGVLNDG
                     TVDTLTPGRLDGVLGPKATAAVHLHELTAHLDLDAFVLFASVTGVWGNGGQAAYAMANA
                     ALDALAEQRRAGGLAATSISWGLWGGGGMAEGNGEVSLNRRGIRALEPATGIEALQRTL
                     DQDTTCRTVVDVDWSEFAPRTAALRRGRLFADLPEARRALGSEGAAQEGAGAAESAATF
                     AERIASLPEPEQRRILVELVRAEAAAVLRHDTTELLAPRRSFKDAGFDSLTALELRNRL
                     STASGVVLPVTVVFDHPNPGALADFLYGEALGLSAQHVAGSDQADTARPAIAPEEPIAI
                     VGMACRYPGEAHSPEELWKLLMDERDVIGPMPTDRGWDVAGLFDPEPGVPGR"
     aSDomain        30..770
                     /ASF="Methylmalonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS37015_PKS_AT.1"
                     /evalue="3.80E-85"
                     /label="IE248_RS37015_PKS_AT.1"
                     /locus_tag="IE248_RS37015"
                     /protein_end="254"
                     /protein_start="7"
                     /score="277.8"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="EAALERVEVLQPASFAVMVSLAALWRSLGVVPDAVVGHSQGEIAA
                     AVVAGGLSLEDGARVVVLRARLIGRELAGHGGMASVALPVTVVEERLERWAGRLGVAVV
                     NGPSATVVAGDTDAVAEFVTVCEAEGVRARRLPVDYASHSAHVETLAAELEEILADVRP
                     VSGEIPFYSTVEAAAIDTASLDAGYWFGNLRRRVRFQETVEQLLADGFPVFVEASAHPV
                     LTAAVQETAESVGRQVCAVGSLRRD"
     aSModule        30..2705
                     /complete
                     /domains="nrpspksdomains_IE248_RS37015_PKS_AT.1"
                     /domains="nrpspksdomains_IE248_RS37015_PKS_KR.1"
                     /domains="nrpspksdomains_IE248_RS37015_PKS_PP.1"
                     /locus_tags="IE248_RS37015"
                     /monomer_pairings="mmal -> Me-ohmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     CDS_motif       39..110
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS37015_0001"
                     /evalue="7.50E-10"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE248_RS37015"
                     /protein_end="34"
                     /protein_start="10"
                     /score="30.9"
                     /tool="antismash"
                     /translation="LERVEVLQPASFAVMVSLAALWRS"
     CDS_motif       114..224
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS37015_0002"
                     /evalue="2.90E-17"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="IE248_RS37015"
                     /protein_end="72"
                     /protein_start="35"
                     /score="54.5"
                     /tool="antismash"
                     /translation="GVVPDAVVGHSQGEIAAAVVAGGLSLEDGARVVVLRA"
     CDS_motif       333..374
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS37015_0003"
                     /evalue="4.00E-03"
                     /label="PKSI-AT-mM_m5"
                     /locus_tag="IE248_RS37015"
                     /protein_end="122"
                     /protein_start="108"
                     /score="9.7"
                     /tool="antismash"
                     /translation="AVVNGPSATVVAGD"
     CDS_motif       441..473
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS37015_0004"
                     /evalue="3.50E-04"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="IE248_RS37015"
                     /protein_end="155"
                     /protein_start="144"
                     /score="13.3"
                     /tool="antismash"
                     /translation="VDYASHSAHVE"
     CDS_motif       579..626
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS37015_0005"
                     /evalue="6.70E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="IE248_RS37015"
                     /protein_end="206"
                     /protein_start="190"
                     /score="15.1"
                     /tool="antismash"
                     /translation="LDAGYWFGNLRRRVRF"
     aSDomain        1644..2165
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS37015_PKS_KR.1"
                     /evalue="5.10E-58"
                     /label="IE248_RS37015_PKS_KR.1"
                     /locus_tag="IE248_RS37015"
                     /protein_end="719"
                     /protein_start="545"
                     /score="187.9"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="GTVLVTGGMGAIGTRVARWLARNGAEHLVLTGRRGAGTPGADELA
                     GELRASGVQVTLAACDVSDRAALAALIDAHPPTAVFHTAGVLNDGTVDTLTPGRLDGVL
                     GPKATAAVHLHELTAHLDLDAFVLFASVTGVWGNGGQAAYAMANAALDALAEQRRAGGL
                     AATSISWGLWG"
     CDS_motif       1647..1745
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS37015_0006"
                     /evalue="4.80E-14"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE248_RS37015"
                     /protein_end="579"
                     /protein_start="546"
                     /score="44.9"
                     /tool="antismash"
                     /translation="TVLVTGGMGAIGTRVARWLARNGAEHLVLTGRR"
     CDS_motif       2010..2120
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS37015_0007"
                     /evalue="3.20E-19"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE248_RS37015"
                     /protein_end="704"
                     /protein_start="667"
                     /score="61.5"
                     /tool="antismash"
                     /translation="LDAFVLFASVTGVWGNGGQAAYAMANAALDALAEQRR"
     aSDomain        2457..2705
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS37015_PKS_PP.1"
                     /evalue="7.60E-29"
                     /label="IE248_RS37015_PKS_PP.1"
                     /locus_tag="IE248_RS37015"
                     /protein_end="899"
                     /protein_start="816"
                     /score="91.9"
                     /tool="antismash"
                     /translation="ASLPEPEQRRILVELVRAEAAAVLRHDTTELLAPRRSFKDAGFDS
                     LTALELRNRLSTASGVVLPVTVVFDHPNPGALADFLYG"
ORIGIN
        1 actggcggtt gtcgcaggtg ctcgatgatg aggctgcgtt ggagcgggtg gaggtgctgc
       61 agccggcctc gtttgcggtg atggtgtcgc ttgccgcgtt gtggcggtcg ttgggtgtgg
      121 tgccggatgc ggtggtgggg cattcgcagg gggagattgc tgccgcggtg gtggcgggtg
      181 gtctgtcgtt ggaggacggg gcccgtgtgg tggtgttgcg tgcacggttg atcgggcgtg
      241 agctggcggg ccatggcggg atggcgtcgg tggcgttgcc ggtgacggtg gtggaggagc
      301 gtctggaacg gtgggcgggg cgtctggggg tggcggtggt caatgggccg tcggccacgg
      361 tggtcgccgg tgacacggac gcggtggcgg agttcgtgac cgtatgtgag gcggagggtg
      421 tgcgggcgcg gcgtctgccg gtggactatg cctcgcactc ggcgcatgtg gagacgctgg
      481 cagccgagct ggaagagatc ctggccgacg tccgtccggt gagcggcgag atcccgttct
      541 actcgacggt ggaggccgcg gcgatcgaca cggcgtcgtt ggatgcgggg tactggttcg
      601 ggaatctgcg tcggcgggtg cggttccagg agacggtcga gcagctgctc gcggatggtt
      661 tcccggtgtt cgtggaggcc agcgcgcacc cggtgctgac agctgcggtg caggagacgg
      721 cggagtccgt gggccggcag gtgtgtgcgg tcggttccct gcgtcgtgac gaggggggtc
      781 tgcggcgttt cctgacctcg gtggcggaag cgttcgtcca gggcgtcgac gtcacctggc
      841 cggccctctt cgacgacacc ggcgcccgca ccatcgacct gcccacctac cccttccagc
      901 accagcgtta ctggcttgag tcgcgtcctt ctgcggcggt tgttccgtcg ggggtgcagg
      961 acggtctgtc gtacgaggtg acgtggaaga gtctgtcgtt gccggagtcg gtgcgtcttg
     1021 acggccggtg gctgctcgtc gtgcccgagg acctggacgc ggacggcaca cgtgtcgccc
     1081 acgacgtcga gcaagccctc accacccacg gcgccacggt ctcccgcctc acggtcgacg
     1141 tgacggcgac ggaccgcacc gacctgtcgg cgcggctcac caccaccgcg gccgaagagc
     1201 aggaacccct tgggggaatc ctgtccctcc tggggtgggc cgagggtgca caggtcgacc
     1261 gcccgaccgt accgattgcc atcgccacct cactggcact ggtgcaggcc gtcggcgacg
     1321 caggattcgg tgttccggtg tgggcggtga cgcggggtgc ggtgtccgtc gtgcctggtg
     1381 aggtgccgga gacggcgggt gcgcagctgt gggcgctcgg ccgcgttgcc gctctcgaac
     1441 tccccgaccg ctggggtggt ctgatcgatc tcccggcgga caccgataca cgtacggcgg
     1501 ctctggcgct gcgggcactg gccgccggga tcgtggccgg tgaggaccag ttggcggtac
     1561 ggccctcggg cgcctacggc cggcgcgtcg ttcaggcagg ccaccggaag cccaagggcg
     1621 cgaagccgga atggcgaccg cgcggcaccg tgctcgtcac cgggggaatg ggcgccatcg
     1681 gaacccgggt ggcccgctgg ctggcccgga acggagccga gcacctggtg ctgaccggcc
     1741 gccgtggtgc cgggacaccc ggcgccgacg agctggccgg agagctgcgg gcgtccggcg
     1801 ttcaggtcac gctcgcggcc tgcgatgtgt ccgatcgtgc cgcgctagcc gcgctgatcg
     1861 acgcgcatcc gccgaccgcc gtcttccaca ccgccggcgt gctgaacgac gggacggtcg
     1921 acacgctcac ccccggacgt ctggacgggg tcctgggccc caaggcgacg gccgccgttc
     1981 acctgcacga gctcaccgca cacctggacc ttgacgcctt cgtcctcttc gcctccgtca
     2041 ccggcgtatg gggtaacggc ggccaggccg cgtacgccat ggccaacgcg gctctggacg
     2101 cgctcgccga gcagcgccgt gccggcggac ttgcggcgac ctccataagc tggggcctct
     2161 ggggcggcgg cggcatggcc gagggcaacg gagaggtgag cctcaaccgc cgtggcatcc
     2221 gcgcgcttga gcccgccacc ggcatcgagg cgctgcaacg gacgctcgac caggacacga
     2281 cctgccgcac cgtcgtcgac gtggactggt ctgagttcgc tccgcgcacg gccgcgctgc
     2341 ggcgcggacg gcttttcgcg gacctgcccg aagcgcgacg tgcgctgggg tcggaagggg
     2401 ccgcgcagga gggtgccggg gccgccgagt cggccgccac atttgccgag cgcattgcgt
     2461 cgctgccgga gcccgaacag cgccggatac tcgtcgagtt ggtccgagcc gaggcggctg
     2521 ccgtcctgcg gcacgacacg acggaactcc tggcgccgcg taggtccttc aaggacgctg
     2581 ggttcgactc gctgaccgcg ctggagctcc gcaaccggct gagtaccgcg agcggtgtcg
     2641 tccttcccgt caccgtcgtc ttcgaccacc cgaaccccgg cgccctggcg gacttcttgt
     2701 acggcgaggc cctgggcctg tccgcgcagc acgtcgccgg gagcgaccag gccgatacgg
     2761 cccggccggc catcgccccc gaagagccga tcgcgatcgt cggtatggcc tgccgttacc
     2821 cgggcgaggc ccactctcct gaggaactgt ggaagttgct catggacgaa cgggacgtca
     2881 tcggccccat gcccactgat cggggttggg atgtggcggg tctgtttgat ccggagcctg
     2941 gtgtgcctgg gcgt
//
